Title:
MONOOXYGENASE MUTANT AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/044987
Kind Code:
A1
Abstract:
The present invention provides a monooxygenase mutant and use thereof. The monooxygenase mutant comprises (a) a protein having an amino acid sequence set forth in SEQ ID NO: 1; or (b) a protein that undergoes an amino acid mutation at at least one site among W60, Y65, D71, Y77, D163, V166, S178, T179, T199, G200, S201, and R222 of the amino acid sequence from (a) and has monooxygenase functions; and (c) a protein having 80% or more homology with the amino acid sequence defined in any one of (a) and (b) and having monooxygenase functions. The present invention can address the problem of low monooxygenase activity in the prior art and applies to the field of enzyme catalysis.
Inventors:
HONG HAO (US)
JAMES GAGE (US)
ZHANG NA (CN)
JIAO XUECHENG (CN)
MA YULEI (CN)
GENG YUHAN (CN)
MU HUIYAN (CN)
JAMES GAGE (US)
ZHANG NA (CN)
JIAO XUECHENG (CN)
MA YULEI (CN)
GENG YUHAN (CN)
MU HUIYAN (CN)
Application Number:
PCT/CN2022/115947
Publication Date:
March 07, 2024
Filing Date:
August 30, 2022
Export Citation:
Assignee:
ASYMCHEM LAB TIANJIN CO LTD (CN)
International Classes:
C12N9/02; C12N1/21; C12N15/53; C12N15/70; C12P11/00; C12P17/00; C12P17/12
Foreign References:
| CN108300707A | 2018-07-20 |
Other References:
DATABASE Protein 31 January 2014 (2014-01-31), ANONYMOUS : "monooxygenase [Rhodococcus jostii RHA1] ", XP093143526, retrieved from NCBI Database accession no. ABG97104.1
DATABASE Nucleotide 31 January 2014 (2014-01-31), ANONYMOUS P: "Rhodococcus jostii RHA1, complete genome", XP093143532, retrieved from NCBI Database accession no. CP000431.1
T. GUL, M. KRZEK, H. P. PERMENTIER, M. W. FRAAIJE, R. BISCHOFF: "Microbial Flavoprotein Monooxygenases as Mimics of Mammalian Flavin-Containing Monooxygenases for the Enantioselective Preparation of Drug Metabolites", DRUG METABOLISM AND DISPOSITION, vol. 44, no. 8, 8 July 2016 (2016-07-08), pages 1270 - 1276, XP055753255, DOI: 10.1124/dmd.115.069104
XIU-QING YANG, XU YANG: "Cytochrome P450 Monooxygenases and Interaction of CYP125A18 with Azole Drugs in Rhodococcus sp.R04", ZHONGGUO SHENGWU HUAXUE YU FENZI SHENGWU XUEBAO - CHINESE JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, BEIJING DAXUE YIXUEBU, CN, vol. 32, no. 3, 20 March 2016 (2016-03-20), CN , pages 295 - 304, XP093143539, ISSN: 1007-7626, DOI: ：10.13865/j．cnki．cjbmb．2016.03.09
DATABASE Nucleotide 31 January 2014 (2014-01-31), ANONYMOUS P: "Rhodococcus jostii RHA1, complete genome", XP093143532, retrieved from NCBI Database accession no. CP000431.1
T. GUL, M. KRZEK, H. P. PERMENTIER, M. W. FRAAIJE, R. BISCHOFF: "Microbial Flavoprotein Monooxygenases as Mimics of Mammalian Flavin-Containing Monooxygenases for the Enantioselective Preparation of Drug Metabolites", DRUG METABOLISM AND DISPOSITION, vol. 44, no. 8, 8 July 2016 (2016-07-08), pages 1270 - 1276, XP055753255, DOI: 10.1124/dmd.115.069104
XIU-QING YANG, XU YANG: "Cytochrome P450 Monooxygenases and Interaction of CYP125A18 with Azole Drugs in Rhodococcus sp.R04", ZHONGGUO SHENGWU HUAXUE YU FENZI SHENGWU XUEBAO - CHINESE JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, BEIJING DAXUE YIXUEBU, CN, vol. 32, no. 3, 20 March 2016 (2016-03-20), CN , pages 295 - 304, XP093143539, ISSN: 1007-7626, DOI: ：10.13865/j．cnki．cjbmb．2016.03.09
Attorney, Agent or Firm:
KANGXIN PARTNERS, P.C. (CN)
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