Title:
MOUSE IN WHICH SITE-SPECIFIC MODIFICATION OF TARGET PROTEIN IS TEMPOROSPATIALLY CONTROLLED, METHOD FOR PRODUCING MOUSE, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2018/044143
Kind Code:
A1
Abstract:
The present invention relates to a mouse (Mus musclus) in which a site-specific modification of a target protein is temporospatially controlled, a method for producing the mouse and use thereof, more specifically to a transgenic mouse in which the expression is controlled of a target protein to which a modification of a particular site is attached in accordance with temporospatial inflow of unnatural amino acid. The mouse capable of temporospatially controlling site-specific modification of a target protein according to the present invention can control the expression of a target protein, to which is attached a site-specific modification, in accordance with the time and/or site at which unnatural amino acid is introduced, and thus the present invention can be useful in the research of target protein function in the body, new drug development, and the like.
Inventors:
PARK HEE-SUNG (KR)
YANG AERIN (KR)
YANG AERIN (KR)
Application Number:
PCT/KR2017/009706
Publication Date:
March 08, 2018
Filing Date:
September 05, 2017
Export Citation:
Assignee:
KOREA ADVANCED INST SCI & TECH (KR)
International Classes:
C12N15/85; A01K67/027; C12N9/00
Domestic Patent References:
| WO2016066995A1 | 2016-05-06 |
Foreign References:
| KR20150070186A | 2015-06-24 | |||
| US20100267087A1 | 2010-10-21 |
Other References:
LEE, SOON JANG: "Expanding the Genetic Code of a mouse'', Ajou University, Molecular Medicine Department of Physiology", MASTERS THESIS, 2015, pages 1 - 22
XIA, XU GANG ET AL.: "An Enhanced U6 Promoter for Synthesis of Short Hairpin RNA", NUCLEIC ACIDS RESEARCH, vol. 31, no. 17, 2003, pages 1 - 5, XP002291074
UMEHARA, TAKUYA ET AL.: "N-Acetyl lysyl-tRNA Synthetases Evolved by a CcdB-based Selection Possess N-acetyl lysine Specificity in Vitro and in Vivo", FEBS LETTERS, vol. 586, no. 6, 28 January 2012 (2012-01-28), pages 729 - 733, XP028472065
DATABASE NCBI 22 May 2015 (2015-05-22), Database accession no. WP _ 01103391.1
HAN, SONG MI ET AL.: "Expanding the Genetic Code of Mus museums", NATURE COMMUNICATIONS, vol. 8, no. 14568, 21 February 2017 (2017-02-21), pages 1 - 7, XP055472293
XIA, XU GANG ET AL.: "An Enhanced U6 Promoter for Synthesis of Short Hairpin RNA", NUCLEIC ACIDS RESEARCH, vol. 31, no. 17, 2003, pages 1 - 5, XP002291074
UMEHARA, TAKUYA ET AL.: "N-Acetyl lysyl-tRNA Synthetases Evolved by a CcdB-based Selection Possess N-acetyl lysine Specificity in Vitro and in Vivo", FEBS LETTERS, vol. 586, no. 6, 28 January 2012 (2012-01-28), pages 729 - 733, XP028472065
DATABASE NCBI 22 May 2015 (2015-05-22), Database accession no. WP _ 01103391.1
HAN, SONG MI ET AL.: "Expanding the Genetic Code of Mus museums", NATURE COMMUNICATIONS, vol. 8, no. 14568, 21 February 2017 (2017-02-21), pages 1 - 7, XP055472293
Attorney, Agent or Firm:
LEE, Cheo Young et al. (KR)
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