Title:
MRNA VECTOR SYSTEM CAPABLE OF EFFICIENTLY EXPRESSING TARGET GENE AND CONSTRUCTION AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/055272
Kind Code:
A1
Abstract:
Provided is an mRNA expression vector capable of efficiently expressing a target protein, the mRNA expression vector, sequentially from the 5' end to the 3' end, comprising: (a) a 5'-UTR element; (b) an open reading frame element encoding the target protein; (c) a 3'-UTR element; and (d) a polyadenylate tail element having a total length of 120 nt or more. Also provided is a construction of the mRNA expression vector, a related product, and use thereof, for example, as an mRNA vaccine.
Inventors:
XU JIANQING (CN)
ZHANG XIAOYAN (CN)
BAI SHIMENG (CN)
ZHANG XIAOYAN (CN)
BAI SHIMENG (CN)
Application Number:
PCT/CN2022/119229
Publication Date:
March 21, 2024
Filing Date:
September 16, 2022
Export Citation:
Assignee:
ZHONG SHAN HOSPITAL FUDAN UNIV (CN)
International Classes:
C12N15/11; A61K31/7105; A61P31/12; A61P35/00; C12N15/67
Domestic Patent References:
| WO2021102107A1 | 2021-05-27 |
Foreign References:
| CN113215178A | 2021-08-06 | |||
| CN114729373A | 2022-07-08 | |||
| CN114507675A | 2022-05-17 | |||
| CN114181962A | 2022-03-15 |
Other References:
TREPOTEC ZELJKA; GEIGER JOHANNES; PLANK CHRISTIAN; ANEJA MANISH K; RUDOLPH CARSTEN: "Segmented poly(A) tails significantly reduce recombination of plasmid DNA without affecting mRNA translation efficiency or half-life", RNA, COLD SPRING HARBOR LABORATORY PRESS, US, vol. 25, no. 4, 31 March 2019 (2019-03-31), US , pages 507 - 518, XP009517039, ISSN: 1355-8382, DOI: 10.1261/rna.069286.118
JUN-HONG YUAN, YANG ZHAO-QING; MA SHAO-HUI: "Progress in research on mRNA vaccines", CHINESE JOURNAL OF BIOLOGICALS, vol. 35, no. 6, 20 June 2022 (2022-06-20), pages 734 - 739, XP093146027, DOI: 10.13200/j.cnki.cjb.003630
JUN-HONG YUAN, YANG ZHAO-QING; MA SHAO-HUI: "Progress in research on mRNA vaccines", CHINESE JOURNAL OF BIOLOGICALS, vol. 35, no. 6, 20 June 2022 (2022-06-20), pages 734 - 739, XP093146027, DOI: 10.13200/j.cnki.cjb.003630
Attorney, Agent or Firm:
SHANGHAI PATENT & TRADEMARK LAW OFFICE, LLC (CN)
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