Title:
MULTI-SIRNA COMPOSITIONS FOR REDUCING GENE EXPRESSION
Document Type and Number:
WIPO Patent Application WO/2011/139843
Kind Code:
A3
Abstract:
This disclosure provides formulations and compounds for use in treating disease by gene silencing and, more specifically, to formulations and compositions having multiple double- stranded nucleic acid complexes, each double- stranded nucleic acid complex comprising an antisense strand and a continuous passenger strand or a discontinuous passenger strand, where the composition decreases expression of one or more mRNA, and to uses of such formulations and compositions to treat or prevent diseases or conditions associated with inappropriate gene expression.
Inventors:
SETH SHAGUNA (US)
FOSNAUGH KATHY L (US)
LIU YAN (US)
TEMPLIN MICHAEL V (US)
FOSNAUGH KATHY L (US)
LIU YAN (US)
TEMPLIN MICHAEL V (US)
Application Number:
PCT/US2011/034354
Publication Date:
January 26, 2012
Filing Date:
April 28, 2011
Export Citation:
Assignee:
MARINA BIOTECH INC (US)
SETH SHAGUNA (US)
FOSNAUGH KATHY L (US)
LIU YAN (US)
TEMPLIN MICHAEL V (US)
SETH SHAGUNA (US)
FOSNAUGH KATHY L (US)
LIU YAN (US)
TEMPLIN MICHAEL V (US)
International Classes:
C12N15/113; A61P35/00
Domestic Patent References:
| WO2010017311A2 | 2010-02-11 | |||
| WO2008147824A2 | 2008-12-04 |
Foreign References:
| US20040029275A1 | 2004-02-12 |
Other References:
YANG DEYONG ET AL: "Therapeutic potential of siRNA-mediated combined knockdown of the IAP genes (Livin, XIAP, and Survivin) on human bladder cancer T24 cells", ACTA BIOCHIMICA ET BIOPHYSICA SINICA, vol. 42, no. 2, February 2010 (2010-02-01), pages 137 - 144, XP002650488
K. TIEMANN ET AL: "Dual-targeting siRNAs", RNA, vol. 16, no. 6, 21 April 2010 (2010-04-21), pages 1275 - 1284, XP055002804, ISSN: 1355-8382, DOI: 10.1261/rna.2005710
GILL CATHERINE ET AL: "Effects of cIAP-1, cIAP-2 and XIAP triple knockdown on prostate cancer cell susceptibility to apoptosis, cell survival and proliferation", MOLECULAR CANCER, BIOMED CENTRAL, LONDON, GB, vol. 8, no. 1, 23 June 2009 (2009-06-23), pages 39, XP021058727, ISSN: 1476-4598, DOI: DOI:10.1186/1476-4598-8-39
JI J ET AL: "Enhanced gene silencing by the application of multiple specific small interfering RNAs", FEBS LETTERS, ELSEVIER, AMSTERDAM, NL, vol. 552, no. 2-3, 25 September 2003 (2003-09-25), pages 247 - 252, XP004460905, ISSN: 0014-5793, DOI: DOI:10.1016/S0014-5793(03)00893-7
T L CHENG ET AL: "Multitarget therapy of malignant cancers by the head-to-tail tandem array multiple shRNAs expression system", CANCER GENE THERAPY, vol. 16, no. 6, 1 June 2009 (2009-06-01), pages 516 - 531, XP055002851, ISSN: 0929-1903, DOI: 10.1038/cgt.2008.102
SOMMER ET AL: "Oncogenic c-H-ras deregulates survivin expression: An improvement for survival", FEBS LETTERS, ELSEVIER, AMSTERDAM, NL, vol. 581, no. 25, 8 October 2007 (2007-10-08), pages 4921 - 4926, XP022289815, ISSN: 0014-5793, DOI: DOI:10.1016/J.FEBSLET.2007.09.023
L. ZHU: "Fibroblast growth factor receptor 3 inhibition by short hairpin RNAs leads to apoptosis in multiple myeloma", MOLECULAR CANCER THERAPEUTICS, vol. 4, no. 5, 1 May 2005 (2005-05-01), pages 787 - 798, XP055001610, ISSN: 1535-7163, DOI: 10.1158/1535-7163.MCT-04-0330
YAN-BIN FENG ET AL: "Overexpression of PLK1 is associated with poor survival by inhibiting apoptosis via enhancement of survivin level in esophageal squamous cell carcinoma", INTERNATIONAL JOURNAL OF CANCER, vol. 124, no. 3, 1 February 2009 (2009-02-01), pages 578 - 588, XP055002858, ISSN: 0020-7136, DOI: 10.1002/ijc.23990
MARIA LAURSEN: "Utilization of unlocked nucleic acid (UNA) to enhance siRNA performance in vitro and in vivo", MOLECULAR BIOSYSTEMS, 9 February 2010 (2010-02-09), pages 862 - 870, XP055000712
K. TIEMANN ET AL: "Dual-targeting siRNAs", RNA, vol. 16, no. 6, 21 April 2010 (2010-04-21), pages 1275 - 1284, XP055002804, ISSN: 1355-8382, DOI: 10.1261/rna.2005710
GILL CATHERINE ET AL: "Effects of cIAP-1, cIAP-2 and XIAP triple knockdown on prostate cancer cell susceptibility to apoptosis, cell survival and proliferation", MOLECULAR CANCER, BIOMED CENTRAL, LONDON, GB, vol. 8, no. 1, 23 June 2009 (2009-06-23), pages 39, XP021058727, ISSN: 1476-4598, DOI: DOI:10.1186/1476-4598-8-39
JI J ET AL: "Enhanced gene silencing by the application of multiple specific small interfering RNAs", FEBS LETTERS, ELSEVIER, AMSTERDAM, NL, vol. 552, no. 2-3, 25 September 2003 (2003-09-25), pages 247 - 252, XP004460905, ISSN: 0014-5793, DOI: DOI:10.1016/S0014-5793(03)00893-7
T L CHENG ET AL: "Multitarget therapy of malignant cancers by the head-to-tail tandem array multiple shRNAs expression system", CANCER GENE THERAPY, vol. 16, no. 6, 1 June 2009 (2009-06-01), pages 516 - 531, XP055002851, ISSN: 0929-1903, DOI: 10.1038/cgt.2008.102
SOMMER ET AL: "Oncogenic c-H-ras deregulates survivin expression: An improvement for survival", FEBS LETTERS, ELSEVIER, AMSTERDAM, NL, vol. 581, no. 25, 8 October 2007 (2007-10-08), pages 4921 - 4926, XP022289815, ISSN: 0014-5793, DOI: DOI:10.1016/J.FEBSLET.2007.09.023
L. ZHU: "Fibroblast growth factor receptor 3 inhibition by short hairpin RNAs leads to apoptosis in multiple myeloma", MOLECULAR CANCER THERAPEUTICS, vol. 4, no. 5, 1 May 2005 (2005-05-01), pages 787 - 798, XP055001610, ISSN: 1535-7163, DOI: 10.1158/1535-7163.MCT-04-0330
YAN-BIN FENG ET AL: "Overexpression of PLK1 is associated with poor survival by inhibiting apoptosis via enhancement of survivin level in esophageal squamous cell carcinoma", INTERNATIONAL JOURNAL OF CANCER, vol. 124, no. 3, 1 February 2009 (2009-02-01), pages 578 - 588, XP055002858, ISSN: 0020-7136, DOI: 10.1002/ijc.23990
MARIA LAURSEN: "Utilization of unlocked nucleic acid (UNA) to enhance siRNA performance in vitro and in vivo", MOLECULAR BIOSYSTEMS, 9 February 2010 (2010-02-09), pages 862 - 870, XP055000712
Attorney, Agent or Firm:
MYLES, Gary, M. et al. (Suite 3400Seattle, WA, US)
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