Title:
MULTI-TARGET DOUBLE-DYE ISOTHERMAL AMPLIFICATION RAPID DETECTION METHOD AND KIT
Document Type and Number:
WIPO Patent Application WO/2021/164050
Kind Code:
A1
Abstract:
A detection method for rapidly detecting a target gene by using multi-target double-dye isothermal amplification technology, a kit and a primer group for use. When the detection method for rapidly detecting a target gene by using multi-target double-dye isothermal amplification technology and a primer mixture of the kit are used, a positive control and a negative control are subjected to an isothermal amplification reaction in a PCR amplification instrument and a color change in a reaction tube is observed with the naked eye at room temperature combined with fluorescence scan reading so as to determine whether a detection object contains target nucleic acid. The instrument for detection has the advantages of having simple requirements, is simple to operate, can adapt to high-concentration and low-concentration virus sample scenarios respectively, has high sensitivity, has strong specificity, results can be determined by means of the naked eye, and has low costs, can adapt to detection for low-concentration and high-concentration samples, and enables basic level and on-site detection to be possible.
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Inventors:
XU DI (CN)
Application Number:
PCT/CN2020/077400
Publication Date:
August 26, 2021
Filing Date:
March 02, 2020
Export Citation:
Assignee:
TIANDZ INC (CN)
International Classes:
C12Q1/70; C12Q1/6844
Domestic Patent References:
| WO2009098789A1 | 2009-08-13 |
Foreign References:
| CN101368204A | 2009-02-18 | |||
| CN110564822A | 2019-12-13 | |||
| CN102344974A | 2012-02-08 | |||
| CN110760569A | 2020-02-07 | |||
| CN101671745A | 2010-03-17 |
Attorney, Agent or Firm:
BS IP LAW OFFICES (CN)
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