Title:
MULTIPLEX PCR SYSTEM
Document Type and Number:
WIPO Patent Application WO/2023/125582
Kind Code:
A1
Abstract:
Provided in the present invention is a method for detecting and differentiating multiple different targets in a single-tube PCR reaction using single-tube ultra-multiplex PCR. The method can avoid false positive signals generated by means of a primer dimer when multiple specific primers exist. Further provided in the present invention are a primer and probe for the single-tube super-multiplex PCR, a reaction system, and a multiple-target detection kit containing the primer, the probe and the reaction system.
Inventors:
ZHAO YUHANG (CN)
HU WAN’ER (CN)
ZHAN HAOMIAO (CN)
HU WAN’ER (CN)
ZHAN HAOMIAO (CN)
Application Number:
PCT/CN2022/142537
Publication Date:
July 06, 2023
Filing Date:
December 27, 2022
Export Citation:
Assignee:
MACCURA BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12N15/11; C12Q1/686
Foreign References:
| US20210381067A1 | 2021-12-09 | |||
| US20180363040A1 | 2018-12-20 | |||
| CN111733293A | 2020-10-02 | |||
| CN109112201A | 2019-01-01 | |||
| CN113528709A | 2021-10-22 | |||
| US20070178476A1 | 2007-08-02 |
Other References:
LIUSHIQI BORJIGIN ET AL.: "A novel real time PCR assay for bovine leukemia virus detection using mixed probes and degenerate primers targeting novel BLV strains", JOURNAL OF VIROLOGICAL METHODS, vol. 297, 16 August 2021 (2021-08-16), XP086820403, DOI: 10.1016/j.jviromet.2021.114264
Attorney, Agent or Firm:
IPSUNNY (CN)
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