Title:
MUTANT ACE2 PROTEIN
Document Type and Number:
WIPO Patent Application WO/2022/045251
Kind Code:
A1
Abstract:
To provide a mutant ACE2 protein having higher bindability to an S protein of a coronavirus. A mutant ACE2 protein including an amino acid sequence A represented by SEQ ID NO: 1 or an amino acid sequence C obtained by mutating an amino acid sequence B having 70% or greater identity with the amino acid sequence A, the amino acid sequence C including a substitution ax of at least two amino acids selected from the group consisting of T20, A25, K26, K31, H34, E35, Q60, N64, S70, T78, L79, N90, T92, and Q101 in the amino acid sequence A or a substitution ay corresponding to the substitution ax in the amino acid sequence B.
Inventors:
HOSHINO ATSUSHI (JP)
MATOBA SATOAKI (JP)
TAKAGI JUNICHI (JP)
OKAMOTO TORU (JP)
MATOBA SATOAKI (JP)
TAKAGI JUNICHI (JP)
OKAMOTO TORU (JP)
Application Number:
PCT/JP2021/031372
Publication Date:
March 03, 2022
Filing Date:
August 26, 2021
Export Citation:
Assignee:
KYOTO PREFECTURAL PUBLIC UNIV CORP (JP)
UNIV OSAKA (JP)
UNIV OSAKA (JP)
International Classes:
C12N15/57; A61K31/7088; A61K35/12; A61K38/48; A61K39/395; A61P31/14; C07K16/00; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/48; C12N15/12; C12N15/13; C12N15/62; C12N15/10
Other References:
CHAN KUI K., DOROSKY DANIELLE, SHARMA PREETI, ABBASI SHAWN A., DYE JOHN M., KRANZ DAVID M., HERBERT ANDREW S., PROCKO ERIK: "Engineering human ACE2 to optimize binding to the spike protein of SARS coronavirus 2", SCIENCE, vol. 369, no. 6508, 4 September 2020 (2020-09-04), US , pages 1261 - 1265, XP055892404, ISSN: 0036-8075, DOI: 10.1126/science.abc0870
GLASGOW ANUM, GLASGOW JEFF, LIMONTA DANIEL, SOLOMON PAIGE, LUI IRENE, ZHANG YANG, NIX MATTHEW A., RETTKO NICHOLAS J., LIM SHION A.: "Engineered ACE2 receptor traps potently neutralize SARS-CoV-2", 4 August 2020 (2020-08-04), pages 1 - 25, XP055903224, Retrieved from the Internet [retrieved on 20220321], DOI: 10.1101/2020.07.31.231746
HIGUCHI YUSUKE; SUZUKI TATSUYA; ARIMORI TAKAO; IKEMURA NARIKO; MIHARA EMIKO; KIRITA YUHEI; OHGITANI ERIKO; MAZDA OSAM; MOTOOKA DAI: "High affinity modified ACE2 receptors prevent SARS-CoV-2 infection", 14 December 2020 (2020-12-14), pages 1 - 23, XP055822015, Retrieved from the Internet DOI: 10.1101/2020.09.16.299891
HIGUCHI YUSUKE, SUZUKI TATSUYA, ARIMORI TAKAO, IKEMURA NARIKO, MIHARA EMIKO, KIRITA YUHEI, OHGITANI ERIKO, MAZDA OSAM, MOTOOKA DAI: "Engineered ACE2 receptor therapy overcomes mutational escape of SARS-CoV-2", NATURE COMMUNICATIONS, vol. 12, no. 1, 21 June 2021 (2021-06-21), pages 1 - 13, XP055868244, DOI: 10.1038/s41467-021-24013-y
MONTEIL VANESSA, KWON HYESOO, PRADO PATRICIA, HAGELKRUYS ASTRID, WIMMER REINER A., STAHL MARTIN, LEOPOLDI ALEXANDRA, GARRETA ELENA: "Inhibition of SARS-CoV-2 Infections in Engineered Human Tissues Using Clinical-Grade Soluble Human ACE2", CELL, vol. 181, no. 4, 14 May 2020 (2020-05-14), Amsterdam NL , pages 905 - 913.e7, XP055790793, ISSN: 0092-8674, DOI: 10.1016/j.cell.2020.04.004
CHANGHAI LEI, KEWEN QIAN, TIAN LI, SHENG ZHANG, WENYAN FU, MIN DING, SHI HU: "Neutralization of SARS-CoV-2 spike pseudotyped virus by recombinant ACE2-Ig", NATURE COMMUNICATIONS, vol. 11, 2070, 24 April 2020 (2020-04-24), England , pages 1 - 5, XP055745525, DOI: 10.1038/s41467-020-16048-4
GLASGOW ANUM, GLASGOW JEFF, LIMONTA DANIEL, SOLOMON PAIGE, LUI IRENE, ZHANG YANG, NIX MATTHEW A., RETTKO NICHOLAS J., LIM SHION A.: "Engineered ACE2 receptor traps potently neutralize SARS-CoV-2", 4 August 2020 (2020-08-04), pages 1 - 25, XP055903224, Retrieved from the Internet
HIGUCHI YUSUKE; SUZUKI TATSUYA; ARIMORI TAKAO; IKEMURA NARIKO; MIHARA EMIKO; KIRITA YUHEI; OHGITANI ERIKO; MAZDA OSAM; MOTOOKA DAI: "High affinity modified ACE2 receptors prevent SARS-CoV-2 infection", 14 December 2020 (2020-12-14), pages 1 - 23, XP055822015, Retrieved from the Internet
HIGUCHI YUSUKE, SUZUKI TATSUYA, ARIMORI TAKAO, IKEMURA NARIKO, MIHARA EMIKO, KIRITA YUHEI, OHGITANI ERIKO, MAZDA OSAM, MOTOOKA DAI: "Engineered ACE2 receptor therapy overcomes mutational escape of SARS-CoV-2", NATURE COMMUNICATIONS, vol. 12, no. 1, 21 June 2021 (2021-06-21), pages 1 - 13, XP055868244, DOI: 10.1038/s41467-021-24013-y
MONTEIL VANESSA, KWON HYESOO, PRADO PATRICIA, HAGELKRUYS ASTRID, WIMMER REINER A., STAHL MARTIN, LEOPOLDI ALEXANDRA, GARRETA ELENA: "Inhibition of SARS-CoV-2 Infections in Engineered Human Tissues Using Clinical-Grade Soluble Human ACE2", CELL, vol. 181, no. 4, 14 May 2020 (2020-05-14), Amsterdam NL , pages 905 - 913.e7, XP055790793, ISSN: 0092-8674, DOI: 10.1016/j.cell.2020.04.004
CHANGHAI LEI, KEWEN QIAN, TIAN LI, SHENG ZHANG, WENYAN FU, MIN DING, SHI HU: "Neutralization of SARS-CoV-2 spike pseudotyped virus by recombinant ACE2-Ig", NATURE COMMUNICATIONS, vol. 11, 2070, 24 April 2020 (2020-04-24), England , pages 1 - 5, XP055745525, DOI: 10.1038/s41467-020-16048-4
Attorney, Agent or Firm:
SAEGUSA & PARTNERS (JP)
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