Title:
MUTANT GLYCINE OXIDASE DERIVED FROM THERMOPHILIC BACTERIUM BELONGING TO FAMILY BACILLUS, AND METHOD FOR PRODUCING SAME
Document Type and Number:
WIPO Patent Application WO/2019/050033
Kind Code:
A1
Abstract:
A mutant glycine oxidase has an amino acid sequence produced by substituting at least one amino acid residue in the wild-type amino acid sequence derived from a thermophilic bacterium belonging to the family Bacillus by another amino acid residue, and has the following enzymatic properties: molecular weight: 40,000 ± 2,000 daltons as measured by SDS-PAGE; optimum temperature: 45°C as measured in the presence of a pyrophosphoric acid salt under the condition of pH 8.5; optimum pH: pH 8.0 as measured in the presence of a pyrophosphoric acid salt under the condition of 37°C; thermal stability: stable at a temperature up to 70°C as measured in the presence of a pyrophosphoric acid salt under the condition of pH 8.5 while retaining for 1 hour; pH stability: stable at a pH value falling within he range from 5.5 to 10.0 as measured in the presence of a pyrophosphoric acid salt under the condition of 4°C while retaining for 24 hours; specific activity: 1.2 unit/mg or more; and a kinetic constant Km: 0.2 mM or less.
Inventors:
KIMURA TAKASHI
TANI YASUSHI
TANI YASUSHI
Application Number:
PCT/JP2018/033455
Publication Date:
March 14, 2019
Filing Date:
September 10, 2018
Export Citation:
Assignee:
NIPRO CORP (JP)
International Classes:
C12N15/31; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/06; C12N15/53; C12N15/63
Domestic Patent References:
| WO2014157705A1 | 2014-10-02 | |||
| WO2014157705A1 | 2014-10-02 |
Other References:
MARTINEZ-MARTINEZ, IRENE ET AL.: "Characterization and structural modeling of a novel thermostable glycine oxidase from Geobacillus kaustophilus HTA426", PROTEINS, vol. 70, no. 4, 2008, pages 1429 - 1441, XP055580926
ROSINI, ELENA ET AL.: "Novel biosensors based on optimized glycine oxidase", THE FEBS JOURNAL, vol. 281, no. 15, 2014, pages 3460 - 3472, XP055580928
YOSHIAKI NISHIYATADAYUKI IMANAKA: "Purification and characterization of a novel glycine oxidase from Bacillus subtilis", FEBS (FEDERATION OF EUROPEAN BIOCHEMICAL SOCIETIES) LETTER, vol. 438, 1998, pages 263 - 266, XP004258603, DOI: 10.1016/S0014-5793(98)01313-1
ELENA ROSINILUCIANO PIUBELLIGIANLUCA MOLLALUCA FRATTINIMATTIA VALENTINOANTONIO VARRIALESABATO D'AURIALOREDANO POLLEGIONI: "Novel biosensors based on oprimized glycine oxidase", FEBS (FEDERATION OF EUROPEAN BIOCHEMICAL SOCIETIES) JOURNAL, vol. 281, 2014, pages 3460 - 3472, XP055580928, DOI: 10.1111/febs.12873
YOSHIAKI NISHIYARYOICHI ARAI: "Crystal structure of glycine oxidase from Geobacillus kaustophilus", PHOTON FACTORY ACTIVITY REPORT 2014 # 32, PART B, USERS' REPORT (BIOLOGICAL SCIENCE, NO.204, 2015
MESSELE YOHANNES EQUARYASUSHI TANIHISAAKI MIHARA: "Purification and Properties of Glycine Oxidase from Pseudomonas putida KT2440", JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY, vol. 61, 2015, pages 506 - 510
See also references of EP 3683313A4
ROSINI, ELENA ET AL.: "Novel biosensors based on optimized glycine oxidase", THE FEBS JOURNAL, vol. 281, no. 15, 2014, pages 3460 - 3472, XP055580928
YOSHIAKI NISHIYATADAYUKI IMANAKA: "Purification and characterization of a novel glycine oxidase from Bacillus subtilis", FEBS (FEDERATION OF EUROPEAN BIOCHEMICAL SOCIETIES) LETTER, vol. 438, 1998, pages 263 - 266, XP004258603, DOI: 10.1016/S0014-5793(98)01313-1
ELENA ROSINILUCIANO PIUBELLIGIANLUCA MOLLALUCA FRATTINIMATTIA VALENTINOANTONIO VARRIALESABATO D'AURIALOREDANO POLLEGIONI: "Novel biosensors based on oprimized glycine oxidase", FEBS (FEDERATION OF EUROPEAN BIOCHEMICAL SOCIETIES) JOURNAL, vol. 281, 2014, pages 3460 - 3472, XP055580928, DOI: 10.1111/febs.12873
YOSHIAKI NISHIYARYOICHI ARAI: "Crystal structure of glycine oxidase from Geobacillus kaustophilus", PHOTON FACTORY ACTIVITY REPORT 2014 # 32, PART B, USERS' REPORT (BIOLOGICAL SCIENCE, NO.204, 2015
MESSELE YOHANNES EQUARYASUSHI TANIHISAAKI MIHARA: "Purification and Properties of Glycine Oxidase from Pseudomonas putida KT2440", JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY, vol. 61, 2015, pages 506 - 510
See also references of EP 3683313A4
Attorney, Agent or Firm:
PATENT CORPORATE BODY ARCO PATENT OFFICE (JP)
Download PDF:
Previous Patent: THERMOPLASTIC ELASTOMER COMPOSITION, FOAM PARTICLE, AND FOAM MOLDED BODY
Next Patent: AUTONOMOUSLY ACTING ROBOT STARING AT PARTNER
Next Patent: AUTONOMOUSLY ACTING ROBOT STARING AT PARTNER