Title:
MUTANT LACTIC BACTERIA WITH A CAPACITY FOR OVEREXPRESSING AT LEAST ONE PEPTIDASE
Document Type and Number:
WIPO Patent Application WO2001029183
Kind Code:
A3
Abstract:
The invention relates to mutants of lactic bacteria such as L. lactis or S. thermophilus which can overexpress one or more peptidases, characterised in that at least one of the negative regulation factors of at least one of the peptidase genes of said bacteria is inactivated, said negative regulation factor being selected from a group comprising the gene codY, the genes of the operon lev, and a gene coding for a protein that is homologous with a beta -glucosidase.
Inventors:
GUEDON ERIC (FR)
ANBA-MONDOLONI JAMILA (FR)
DELORME CHRISTINE (FR)
RENAULT PIERRE (FR)
ANBA-MONDOLONI JAMILA (FR)
DELORME CHRISTINE (FR)
RENAULT PIERRE (FR)
Application Number:
PCT/FR2000/002869
Publication Date:
November 22, 2001
Filing Date:
October 13, 2000
Export Citation:
Assignee:
AGRONOMIQUE INST NAT RECH (FR)
GUEDON ERIC (FR)
ANBA MONDOLONI JAMILA (FR)
DELORME CHRISTINE (FR)
RENAULT PIERRE (FR)
GUEDON ERIC (FR)
ANBA MONDOLONI JAMILA (FR)
DELORME CHRISTINE (FR)
RENAULT PIERRE (FR)
International Classes:
C07K14/315; C12N1/21; C12N9/52; (IPC1-7): C12N1/20; C12Q1/68; A23C19/032
Other References:
KUNJI EDMUND R S ET AL: "Transport of beta-Casein-derived Peptides by the Oligopeptide Transport System Is a Crucial Step in the Proteolytic Pathway of Lactococcus lactis.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 270, no. 4, 1995, pages 1569 - 1574, XP002141676, ISSN: 0021-9258
MARUGG JOEY D ET AL: "Medium-dependent regulation of proteinase gene expression in Lactococcus lactis: Control of transcription initiation by specific dipeptides.", JOURNAL OF BACTERIOLOGY, vol. 177, no. 11, 1995, pages 2982 - 2989, XP002141677, ISSN: 0021-9193
FISHER SUSAN H ET AL: "Role of CodY in regulation of the Bacillus subtilis hut operon.", JOURNAL OF BACTERIOLOGY, vol. 178, no. 13, 1996, pages 3779 - 3784, XP002160377, ISSN: 0021-9193
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; April 1999 (1999-04-01), FISHER SUSAN H: "Regulation of nitrogen metabolism in Bacillus subtilis: Vive la difference!", XP002160389, Database accession no. PREV199900269050
KUNJI E ET AL: "The proteolytic systems of lactic acid bacteria", ANTONIE VAN LEEUWENHOEK, vol. 70, 1996, pages 187 - 221, XP000914826
MARUGG JOEY D ET AL: "Medium-dependent regulation of proteinase gene expression in Lactococcus lactis: Control of transcription initiation by specific dipeptides.", JOURNAL OF BACTERIOLOGY, vol. 177, no. 11, 1995, pages 2982 - 2989, XP002141677, ISSN: 0021-9193
FISHER SUSAN H ET AL: "Role of CodY in regulation of the Bacillus subtilis hut operon.", JOURNAL OF BACTERIOLOGY, vol. 178, no. 13, 1996, pages 3779 - 3784, XP002160377, ISSN: 0021-9193
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; April 1999 (1999-04-01), FISHER SUSAN H: "Regulation of nitrogen metabolism in Bacillus subtilis: Vive la difference!", XP002160389, Database accession no. PREV199900269050
KUNJI E ET AL: "The proteolytic systems of lactic acid bacteria", ANTONIE VAN LEEUWENHOEK, vol. 70, 1996, pages 187 - 221, XP000914826
Download PDF: