Title:
MUTANT POLYHYDROXYALKANOIC ACID SYNTHASE, GENE AND TRANSFORMANT THEREOF, AND METHOD FOR PRODUCING POLYHYDROXYALKANOIC ACID
Document Type and Number:
WIPO Patent Application WO/2019/142717
Kind Code:
A1
Abstract:
Provided is a mutant PHA synthase that achieves production of a PHA copolymer having a high or low 3HH ratio while maintaining PHA productivity. Mutant polyhydroxyalkanoic acid synthase having an amino acid sequence that exhibits 85% or more sequence identity with an amino acid sequence shown by SEQ ID NO: 1 and that includes one or more mutations from among (a)-(c) below. Mutation (a): a mutation in which serine 389 from the N-terminal of the amino acid sequence shown by SEQ ID NO: 1 is substituted by an amino acid other than serine; mutation (b): a mutation in which leucine 436 from the N-terminal of the amino acid sequence shown by SEQ ID NO: 1 is substituted by an amino acid other than leucine; mutation (c): a mutation in which 11-19 amino acid residues are deleted from the C-terminal of the amino acid sequence shown by SEQ ID NO: 1.
Inventors:
KOBAYASHI SHINGO
YOSHIDA SHINICHI
SATO SHUNSUKE
TAOKA NAOAKI
YOSHIDA SHINICHI
SATO SHUNSUKE
TAOKA NAOAKI
Application Number:
PCT/JP2019/000453
Publication Date:
July 25, 2019
Filing Date:
January 10, 2019
Export Citation:
Assignee:
KANEKA CORP (JP)
International Classes:
C12N15/60; C12N1/21; C12N9/88; C12P7/62
Domestic Patent References:
| WO2003050277A1 | 2003-06-19 | |||
| WO2017104722A1 | 2017-06-22 | |||
| WO2017056442A1 | 2017-04-06 |
Foreign References:
| JP2015077103A | 2015-04-23 | |||
| JP2007259708A | 2007-10-11 |
Other References:
KICHISE, T. ET AL.: "Enhanced accumulation and changed monomer composition in polyhydroxyalkanoate (PHA) copolyester by in vitro evolution of Aeromonas caviae PHA synthase", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 68, no. 5, 2002, pages 2411 - 2419, XP003000947, ISSN: 0099-2240, doi:10.1128/AEM.68.5.2411-2419.2002
TSUGE, T. ET AL.: "Combination of N149S and D171G mutations in Aeromonas caviae polyhydroxyalkanoate synthase and impact on polyhydroxyalkanoate biosynthesis", FEMS MICROBIOL. LETT., vol. 277, 2007, pages 217 - 222, XP002702996, ISSN: 0378-1097, doi:10.1111/J.1574-6968.2007.00958.X
T. KICHISES. TAGUCHIY DOI, APP. ENVIRON. MICROBIOL., vol. 68, 2002, pages 2411 - 2419
T. TSUGES. WATANABED. SHIMADAH. ABEY DOIS. TAGUCHI, FEMS MICROBIOL. LETT., vol. 277, 2007, pages 217 - 222
OHMAN ET AL., J. BACTERIOL., vol. 162, 1985, pages 1068
NOTI ET AL., METHODS ENZYMOL., vol. 154, 1987, pages 197
MOL. MICROBIOL., vol. 6, 1992, pages 1195
LENZ ET AL., J. BACTERIOL., vol. 176, 1994, pages 4385
SAMBROOK, J. ET AL.: "Molecular Cloning, A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
TSUGE, T. ET AL.: "Combination of N149S and D171G mutations in Aeromonas caviae polyhydroxyalkanoate synthase and impact on polyhydroxyalkanoate biosynthesis", FEMS MICROBIOL. LETT., vol. 277, 2007, pages 217 - 222, XP002702996, ISSN: 0378-1097, doi:10.1111/J.1574-6968.2007.00958.X
T. KICHISES. TAGUCHIY DOI, APP. ENVIRON. MICROBIOL., vol. 68, 2002, pages 2411 - 2419
T. TSUGES. WATANABED. SHIMADAH. ABEY DOIS. TAGUCHI, FEMS MICROBIOL. LETT., vol. 277, 2007, pages 217 - 222
OHMAN ET AL., J. BACTERIOL., vol. 162, 1985, pages 1068
NOTI ET AL., METHODS ENZYMOL., vol. 154, 1987, pages 197
MOL. MICROBIOL., vol. 6, 1992, pages 1195
LENZ ET AL., J. BACTERIOL., vol. 176, 1994, pages 4385
SAMBROOK, J. ET AL.: "Molecular Cloning, A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
Attorney, Agent or Firm:
PATENT CORPORATE BODY ARCO PATENT OFFICE (JP)
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