Title:
MUTANT STRAIN HAVING ENHANCED L-GLUTAMIC ACID PRODUCING ABILITY, AND L-GLUTAMIC ACID PREPARATION METHOD USING SAME
Document Type and Number:
WIPO Patent Application WO/2020/122505
Kind Code:
A1
Abstract:
The present invention relates to a mutant strain having enhanced L-glutamic acid producing ability, and an L-glutamic acid preparation method using same. A mutant strain according to one specific example of the present invention has reduced or inactivated citramalate synthase activity and thus exhibits a reduced yield of citramalate which is a byproduct, and exhibits excellent L-glutamic acid producing ability. A strain having the YggB protein additionally mutated increases the discharge of glutamic acid and thus allows an enhanced product yield of the L-glutamic acid. Therefore, L-glutamic acid can be more effectively prepared by means of the mutant strain.
Inventors:
KIM HYUN YOUNG (KR)
LEE SUN HEE (KR)
KIM HYUN HO (KR)
JO YOUNG IL (KR)
YOUN HYUNG SEOP (KR)
KIM SUOK SU (KR)
LEE SUN HEE (KR)
KIM HYUN HO (KR)
JO YOUNG IL (KR)
YOUN HYUNG SEOP (KR)
KIM SUOK SU (KR)
Application Number:
PCT/KR2019/017124
Publication Date:
June 18, 2020
Filing Date:
December 05, 2019
Export Citation:
Assignee:
DAESANG CORP (KR)
International Classes:
C12N15/77; C12N9/10; C12P13/00
Domestic Patent References:
| WO2006070944A2 | 2006-07-06 |
Foreign References:
| KR20150003752A | 2015-01-09 | |||
| EP1491625B1 | 2010-07-21 | |||
| JP2018057376A | 2018-04-12 | |||
| US6852516B2 | 2005-02-08 | |||
| US6962805B2 | 2005-11-08 | |||
| KR101138289B1 | 2012-04-24 | |||
| KR20110034718A | 2011-04-06 |
Other References:
VOGT, M. ET AL.: "Pushing product formation to its limit: metabolic engineering of Corynebacterium glutamicum for L-leucine overproduction", METABOLIC ENGINEERING, vol. 22, 11 December 2013 (2013-12-11), pages 40 - 52, XP055111480, DOI: 10.1016/j.ymben.2013.12.001
NAKAMURA, J. ET AL.: "Mutations of the Corynebacterium glutamicum NCgl 1221 gene , encoding a mechanosensitive channel homolog, induce L-glutamic acid production", APPLIED ENVIRONMENTAL MICROBIOLOGY, vol. 73, no. 14, July 2007 (2007-07-01), pages 4491 - 4498, XP002691368, DOI: 10.1128/AEM.02446-06
FEMS MICROBIOL LETT., vol. 218, no. 2, 2003, pages 305 - 309
MOL MICROBIOL., vol. 54, no. 2, 2004, pages 420 - 438
EMBO J., vol. 18, no. 7, 1999, pages 1730 - 7
"Genbank", Database accession no. NP_600492
"Manual of Methods for General Bacteriology", 1981, AMERICAN SOCIETY FOR BACTERIOLOGY
GENE, vol. 145, 1994, pages 69 - 73
APPL. MICROBIOL. BIOTECHNOL., vol. 52, 1999, pages 541 - 545
See also references of EP 3896165A4
NAKAMURA, J. ET AL.: "Mutations of the Corynebacterium glutamicum NCgl 1221 gene , encoding a mechanosensitive channel homolog, induce L-glutamic acid production", APPLIED ENVIRONMENTAL MICROBIOLOGY, vol. 73, no. 14, July 2007 (2007-07-01), pages 4491 - 4498, XP002691368, DOI: 10.1128/AEM.02446-06
FEMS MICROBIOL LETT., vol. 218, no. 2, 2003, pages 305 - 309
MOL MICROBIOL., vol. 54, no. 2, 2004, pages 420 - 438
EMBO J., vol. 18, no. 7, 1999, pages 1730 - 7
"Genbank", Database accession no. NP_600492
"Manual of Methods for General Bacteriology", 1981, AMERICAN SOCIETY FOR BACTERIOLOGY
GENE, vol. 145, 1994, pages 69 - 73
APPL. MICROBIOL. BIOTECHNOL., vol. 52, 1999, pages 541 - 545
See also references of EP 3896165A4
Attorney, Agent or Firm:
PCR INTELLECTUAL PROPERTY LAW FIRM (KR)
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