Title:
MUTANT TRANSPOSON VECTOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2007/004642
Kind Code:
A1
Abstract:
Disclosed are a mutant transposon vector and a method for introducing a foreign gene into a cell. A mutant transposon vector having a nucleic acid fragment inserted therein, the nucleic acid fragment having the properties (i) and (ii) or the properties (i) to (iii) below; a foreign gene-expressing cell which is prepared by introducing the mutant transposon vector into the chromosomal DNA of an established cell or a cell capable of being individuated and then additionally introducing a foreign gene-expressing cassette into a recombinant system (e.g., Cre-Lox system); a recombinant animal created by using the cell (in the case where the cell capable of being individuated is used); and a method for producing a foreign protein from the cell or the recombinant animal: (i) comprising a 5'-TIR sequence and a 3'-TIR sequence of transposon gene; (ii) having a sequence at which a recombination reaction occurs inserted between at least one set of DR regions of two sets of DR regions present in a 5'-TIR sequence or a 3'-TIR sequence of transposon gene; and (iii) having a restriction recognition site or a foreign gene-expressing cassette inserted between a 5'-TIR sequence and a 3'-TIR sequence of transposon gene.
Inventors:
KAMINAKA KAZUYOSHI (JP)
MAEDA HIROAKI (JP)
HIRASHIMA MASAKI (JP)
KAWAMURA RYOICHI (JP)
MATSUDA JUNICHI (JP)
KUWANA TAKASHI (JP)
MAEDA HIROAKI (JP)
HIRASHIMA MASAKI (JP)
KAWAMURA RYOICHI (JP)
MATSUDA JUNICHI (JP)
KUWANA TAKASHI (JP)
Application Number:
PCT/JP2006/313302
Publication Date:
January 11, 2007
Filing Date:
July 04, 2006
Export Citation:
Assignee:
CHEMO SERO THERAPEUT RES INST (JP)
KAMINAKA KAZUYOSHI (JP)
MAEDA HIROAKI (JP)
HIRASHIMA MASAKI (JP)
KAWAMURA RYOICHI (JP)
MATSUDA JUNICHI (JP)
KUWANA TAKASHI (JP)
KAMINAKA KAZUYOSHI (JP)
MAEDA HIROAKI (JP)
HIRASHIMA MASAKI (JP)
KAWAMURA RYOICHI (JP)
MATSUDA JUNICHI (JP)
KUWANA TAKASHI (JP)
International Classes:
C12N15/09; A01K67/027; C12N5/10
Domestic Patent References:
| WO2003031629A1 | 2003-04-17 |
Other References:
IVICS Z. ET AL.: "Identification of functional domains and evaluation of Tcl-like transposable elements", PROC. NATL. ACAD. SCI. USA, vol. 93, no. 10, 14 May 1996 (1996-05-14), pages 5008 - 5013, XP002072767
CANESTRO C. ET AL.: "Isolation and characterization of the first non-autonomous transposable element in amphioxus, ATE-1", GENE, vol. 318, 2003, pages 69 - 73, XP004470271
ARAKI K. ET AL.: "Exchangeable gene trap using the Cre/mutated lox system", CELL MOL. BIOL., vol. 45, no. 5, July 1999 (1999-07-01), pages 737 - 750, XP000877476
SHIMIZU T. ET AL.: "Identification of cis-regulatory sequences in the human angiotensinogen gene by transgene coplacement and site-specific recombination", MOL. CELL BIOL., vol. 25, no. 8, April 2005 (2005-04-01), pages 2938 - 2945, XP003002683
CHATTERJEE P.K. ET AL.: "Selecting transpositions using phase P1 headful packaging: new markerless transposons for functionally mapping long-range regulatory sequences in bacterial artificial chromosomes and P1-derived artificial chromosomes", ANAL. BIOCHEM., vol. 335, no. 2, 2004, pages 305 - 315, XP004642089
CANESTRO C. ET AL.: "Isolation and characterization of the first non-autonomous transposable element in amphioxus, ATE-1", GENE, vol. 318, 2003, pages 69 - 73, XP004470271
ARAKI K. ET AL.: "Exchangeable gene trap using the Cre/mutated lox system", CELL MOL. BIOL., vol. 45, no. 5, July 1999 (1999-07-01), pages 737 - 750, XP000877476
SHIMIZU T. ET AL.: "Identification of cis-regulatory sequences in the human angiotensinogen gene by transgene coplacement and site-specific recombination", MOL. CELL BIOL., vol. 25, no. 8, April 2005 (2005-04-01), pages 2938 - 2945, XP003002683
CHATTERJEE P.K. ET AL.: "Selecting transpositions using phase P1 headful packaging: new markerless transposons for functionally mapping long-range regulatory sequences in bacterial artificial chromosomes and P1-derived artificial chromosomes", ANAL. BIOCHEM., vol. 335, no. 2, 2004, pages 305 - 315, XP004642089
Attorney, Agent or Firm:
KAWAMIYA, Osamu et al. (IMP Building 3-7, Shiromi 1-chome, Chuo-k, Osaka-shi Osaka 01, JP)
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