Title:
NANOPROBE FOR MEASURING INTRACELLULAR PH, AND METHOD AND APPARATUS FOR MEASURING PH IN SINGLE CELL USING SAME
Document Type and Number:
WIPO Patent Application WO/2022/164262
Kind Code:
A1
Abstract:
The present invention relates to a method and apparatus for measuring pH in a single cell, and a method for manufacturing a nanoprobe for same. The method for measuring pH in a single cell comprises (a) inserting, into the single cell, a nanoprobe formed by coupling a pH-responsive fluorescent material capable of responding to pH to a nanowire grown on a tapered tip of an optical fiber, (b) shining light on the nanoprobe through the optical fiber, (c) exciting the pH-responsive fluorescent substance by means of the light to generate fluorescence, (d) acquiring, through the optical fiber, a fluorescence signal generated from the fluorescent material according to the pH of the cell, and (e) obtaining the pH value in the cell by analyzing the fluorescence signal. The method for manufacturing a nanoprobe comprises (a) filling a nanopipette with a nanowire material solution and lowering the nanopipette to bring the nanowire material solution into contact with an end of an optical fiber, (b) raising the nanopipette to grow a nanowire on the end of the optical fiber, (c) filling a micropipette with an aqueous solution containing a pH-responsive fluorescent substance and lowering the micropipette so that a portion of the nanowire is immersed in the aqueous solution, and (d) raising the micropipette to form a nanoprobe labeled with the pH-responsive fluorescent substance.
Inventors:
JE JUNG HO (KR)
OH SEUNG SOO (KR)
YONG MOON JUNG (KR)
YANG UN (KR)
KANG BYUNG HWA (KR)
OH SEUNG SOO (KR)
YONG MOON JUNG (KR)
YANG UN (KR)
KANG BYUNG HWA (KR)
Application Number:
PCT/KR2022/001600
Publication Date:
August 04, 2022
Filing Date:
January 28, 2022
Export Citation:
Assignee:
POSTECH RES & BUSINESS DEV FOUND (KR)
International Classes:
G01N21/64; G01N21/80; G01N33/483; G01Q60/38
Foreign References:
| KR101749071B1 | 2017-06-21 | |||
| JP2009161427A | 2009-07-23 | |||
| JP2010203875A | 2010-09-16 | |||
| US20180045675A1 | 2018-02-15 |
Other References:
YANG QINGBO, XIAOBEI ZHANG,YANG SONG,KE LI,HONGLAN SHI,HAI XIAO,YINFA MA: "Label‐free in situ pH monitoring in a single living cell using an optical nanoprobe", MEDICAL DEVICES & SENSORS, vol. 3, no. 3, 12 March 2020 (2020-03-12), pages e10079, XP055954334, DOI: 10.1002/mds3.10079
Attorney, Agent or Firm:
SESHIN PATENT & LAW FIRM (KR)
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