Title:
NEW ANTIFUNGAL PREPARATIONS, PROCESS FOR MAKING SUCH PREPARATIONS, PROCESS FOR OBTAINING PLANTS WITH DECREASED SUSCEPTIBILITY TO FUNGI
Document Type and Number:
WIPO Patent Application WO1991018984
Kind Code:
A3
Abstract:
New methods are provided for the identification and purification of proteins from plants with an inhibitory effect on pathogens and/or pests, such as insects, nematodes, fungi, and bacteria, using a combination of non-denaturing protein fractionation techniques on leaf-extracts of plants which were treated to develop induced resistance and an antipathogenic assay. Also provided are antifungal proteins obtained by the said method, recombinant nucleotide sequences encoding the said proteins, as well as plants with reduced susceptibility to fungi due to production and desired targetting of the said proteins in the plant.
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Inventors:
WOLOSHUK CHARLES PETER (NL)
MELCHERS LEO SJOERD (NL)
CORNELISSEN BERNARDUS JOHANNES (NL)
MEULENHOFF ELISABETH JOSINE SO (NL)
SELA-BUURLAGE MARIANNE BEATRIX (NL)
VAN DEN ELZEN PETRUS JOSEPHUS (NL)
MELCHERS LEO SJOERD (NL)
CORNELISSEN BERNARDUS JOHANNES (NL)
MEULENHOFF ELISABETH JOSINE SO (NL)
SELA-BUURLAGE MARIANNE BEATRIX (NL)
VAN DEN ELZEN PETRUS JOSEPHUS (NL)
Application Number:
PCT/NL1991/000089
Publication Date:
May 14, 1992
Filing Date:
May 31, 1991
Export Citation:
Assignee:
MOGEN INT (NL)
International Classes:
A01N63/00; A01N63/02; A01N65/00; A01N65/08; A01N65/10; A01N65/20; A01H5/00; A01N65/38; A61K38/00; A61P31/04; C07K1/14; C07K1/20; C07K14/415; C12N5/10; C12N15/09; C12N15/29; C12N15/82; C12P21/00; C12R1/91; (IPC1-7): C12N15/29; A61K37/02; A01H5/00; A01N65/00; C12N15/82; C12N5/10
Domestic Patent References:
| WO1989002744A1 | 1989-04-06 | |||
| WO1990003725A1 | 1990-04-19 | |||
| WO1990007001A1 | 1990-06-28 |
Foreign References:
| EP0351924A2 | 1990-01-24 | |||
| EP0309862A1 | 1989-04-05 | |||
| EP0292435A1 | 1988-11-23 | |||
| EP0270248A2 | 1988-06-08 | |||
| DD295639A5 | 1991-11-07 | |||
| EP0392225A2 | 1990-10-17 | |||
| EP0375091A1 | 1990-06-27 | |||
| EP0332104A2 | 1989-09-13 | |||
| EP0440304A1 | 1991-08-07 |
Other References:
Plant Physiol., vol. 90, July 1989, N.K. Singh et al.: "Molecular cloning of osmotin and regulation of its expression by ABA and adaption to low water potential", pages 1096-1101, see the whole article
Biochimica Biophysica Acta, vol. 880, 1986, Elsevier Science Publishers B.V., W.K. Roberts et al.: "Isolation and partial characterization of two antifungal proteins from barley", pages 161-170, see the whole article
Molecular Plant-Microbe Interactions, vol. 3, no. 4, 1990, The American Phytopathological Society, H.J.M. Linthorst et al.: "Analysis of acidic and basic chitinases from tobacco and petunia and their constitutive expression in transgenic tobacco", pages 252-258, see page 257, left-hand column, last paragraph
Journal of Cellular Biochemistry, supplement 14E, Symposium 16-22 April 1990, R. BROGLIE et al.: "Chitinase expression in transgenic tobacco plants: increased protection against a soil-borne fungal pathogen", page 276, see abstract R 108
Proc. Natl. Acad. Sci. USA, vol. 86, December 1989, R. Johnson et al.: "Expression of proteinase inhibitors I and II in transgenic tobacco plants: Effects on natural defense against Manduca sexta larvae", pages 9871-9875, see the whole article
The EMBO Journal, vol. 7, no. 6, June 1988, IRL Press Ltd, (Oxford, GB), H. BOHLMANN et al.: "Leaf-specific thionins of barley - a novel class of cell wall proteins toxic to plant-pathogenic fungi and possibly involved in the defence mechanism of plants", pages 1559-1565, see page 1562 - page 1563, left-hand column
Plant Molecular Biology, vol. 9, 1987, Martinus Nijhoff Publishers, (Dordrecht, NL), D.B. Collinge et al.: "Plant gene expression in response to pathogens", pages 389-410, see page 392, right-hand column, lines 12-18
The Plant Cell, vol. 2, no. 11, December 1990, American Society of Plant Physiologists, S.Y. BEDNAREK et al.: "A carboxyl-terminal propeptide is necessary for proper sorting of barley lectin to vacuoles of tobacco", pages 1145-1155, see the whole document
Journal of Cellular Biochemistry, Keystone Symposia on Molecular & Cellular Biology, supplement 15A, 10th -24th January 1991, abstract no. A 015, J.F. Bol et al.: "Gene control by insects, pathogens, and environment: Expression and targeting of tobacco proteins by virus infection", page 9, see abstract A 015
Plant Cell Physiol., vol. 31, no. 2, 1990, S. Takeda et al.: "Characterization of polypeptides that accumulate in cultured Nicotiana tabacum cells", pages 215-221, see page 219, right-hand column - page 220, left-hand column
Physiological Plant Pathology, vol. 15, no. 3, November 1979, Academic Press, J.L. McIntyre et al.: "Induction of localized and systemic protection against Phytophthora parasitica var. nicotianae by tobacco mosaic virus infection of tobacco hypersensitive to the virus", pages 321-330, see the whole article
Physiological and Molecular Plant Pathology, vol. 35, 1989, Academic Press Ltd, U. Christ et al.: "Pathogenesis-related proteins of tomato: I. Induction by Phytophthora infestans and other biotic and abiotic inducers and correlations with resistance", pages 53-65, see the whole article
Nature, vol. 324, no. 6095, 27th November - 3rd December 1986, (Neptune, NJ, US), A. Schlumbaum et al.: "Plant chitinases are potent inhibitors of fungal growth", pages 365-367, see page 366, right-hand column
Chemical Abstracts, vol. 110, 1989, (Columbus, Ohio, US), F. Mauch et al.: "Antifungal hydrolases in pea tissue. II. Inhibition of fungal growth by combinations of chitinase and beta-1,3-glucanase", see page 451, abstract 4848v, & Plant. Physiol. 1988, 88(3), 936-42
Biochimica Biophysica Acta, vol. 880, 1986, Elsevier Science Publishers B.V., W.K. Roberts et al.: "Isolation and partial characterization of two antifungal proteins from barley", pages 161-170, see the whole article
Molecular Plant-Microbe Interactions, vol. 3, no. 4, 1990, The American Phytopathological Society, H.J.M. Linthorst et al.: "Analysis of acidic and basic chitinases from tobacco and petunia and their constitutive expression in transgenic tobacco", pages 252-258, see page 257, left-hand column, last paragraph
Journal of Cellular Biochemistry, supplement 14E, Symposium 16-22 April 1990, R. BROGLIE et al.: "Chitinase expression in transgenic tobacco plants: increased protection against a soil-borne fungal pathogen", page 276, see abstract R 108
Proc. Natl. Acad. Sci. USA, vol. 86, December 1989, R. Johnson et al.: "Expression of proteinase inhibitors I and II in transgenic tobacco plants: Effects on natural defense against Manduca sexta larvae", pages 9871-9875, see the whole article
The EMBO Journal, vol. 7, no. 6, June 1988, IRL Press Ltd, (Oxford, GB), H. BOHLMANN et al.: "Leaf-specific thionins of barley - a novel class of cell wall proteins toxic to plant-pathogenic fungi and possibly involved in the defence mechanism of plants", pages 1559-1565, see page 1562 - page 1563, left-hand column
Plant Molecular Biology, vol. 9, 1987, Martinus Nijhoff Publishers, (Dordrecht, NL), D.B. Collinge et al.: "Plant gene expression in response to pathogens", pages 389-410, see page 392, right-hand column, lines 12-18
The Plant Cell, vol. 2, no. 11, December 1990, American Society of Plant Physiologists, S.Y. BEDNAREK et al.: "A carboxyl-terminal propeptide is necessary for proper sorting of barley lectin to vacuoles of tobacco", pages 1145-1155, see the whole document
Journal of Cellular Biochemistry, Keystone Symposia on Molecular & Cellular Biology, supplement 15A, 10th -24th January 1991, abstract no. A 015, J.F. Bol et al.: "Gene control by insects, pathogens, and environment: Expression and targeting of tobacco proteins by virus infection", page 9, see abstract A 015
Plant Cell Physiol., vol. 31, no. 2, 1990, S. Takeda et al.: "Characterization of polypeptides that accumulate in cultured Nicotiana tabacum cells", pages 215-221, see page 219, right-hand column - page 220, left-hand column
Physiological Plant Pathology, vol. 15, no. 3, November 1979, Academic Press, J.L. McIntyre et al.: "Induction of localized and systemic protection against Phytophthora parasitica var. nicotianae by tobacco mosaic virus infection of tobacco hypersensitive to the virus", pages 321-330, see the whole article
Physiological and Molecular Plant Pathology, vol. 35, 1989, Academic Press Ltd, U. Christ et al.: "Pathogenesis-related proteins of tomato: I. Induction by Phytophthora infestans and other biotic and abiotic inducers and correlations with resistance", pages 53-65, see the whole article
Nature, vol. 324, no. 6095, 27th November - 3rd December 1986, (Neptune, NJ, US), A. Schlumbaum et al.: "Plant chitinases are potent inhibitors of fungal growth", pages 365-367, see page 366, right-hand column
Chemical Abstracts, vol. 110, 1989, (Columbus, Ohio, US), F. Mauch et al.: "Antifungal hydrolases in pea tissue. II. Inhibition of fungal growth by combinations of chitinase and beta-1,3-glucanase", see page 451, abstract 4848v, & Plant. Physiol. 1988, 88(3), 936-42
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