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Title:
A NEW KANAMYCIN COMPOUND, KANAMYCIN-PRODUCING STREPTOMYCES SPECIES BACTERIUM, AND METHOD OF PRODUCING KANAMYCIN
Document Type and Number:
WIPO Patent Application WO/2011/132830
Kind Code:
A1
Abstract:
Vectors expressing kanA - kanB - kanK and other kanamycin production-related genes, Streptomyces species recombinant bacteria transformed with the vectors, a method of producing kanamycin antibiotics by the bacteria, and a new kanamycin compound produced by the bacterium are provided. With the use of the recombinant bacteria of the present invention, the direct fermentative biosynthesis of amikacin and tobramycin as semi-synthetic kanamycins is possible, and the yield of kanamycin B as a precursor of the semi-synthetic kanamycin is improved.

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Inventors:
YOON YEO JOON (KR)
PARK SUNG RYEOL (KR)
PARK JE WON (KR)
SOHNG JAE KYUNG (KR)
Application Number:
PCT/KR2010/007041
Publication Date:
October 27, 2011
Filing Date:
October 14, 2010
Export Citation:
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Assignee:
UNIV EWHA IND COLLABORATION (KR)
YOON YEO JOON (KR)
PARK SUNG RYEOL (KR)
PARK JE WON (KR)
SOHNG JAE KYUNG (KR)
International Classes:
C12N15/76; C12N1/21; C12N15/31; C12P1/06; C12R1/465
Foreign References:
KR910003096A1991-02-26
KR20030013450A2003-02-14
KR20100007041A2010-01-22
Other References:
PARK: "Characterization of the biosynthetic route to 4,6-disubstituted amino glycosides using heterologous expression system", PH.D. DISSERTATION, 28 February 2010 (2010-02-28), XP008164240
NEPAL ET AL.: "Heterologous production of parmamine in streptomyces lividans TK24 using kanamycin biosynthetic genes from streptomyces kanamyceticus ATC C12853", MOLECULES AND CELL, vol. 27, 31 May 2009 (2009-05-31), pages 601 - 608, XP008164168
See also references of EP 2561080A4
NEPAL ET AL., MOBCEHS, vol. 29, no. 5, 2009, pages 601 - 8
Attorney, Agent or Firm:
KIM, Soon Woong (188-5 Guro-dong Guro-gu, Seoul 152-050, KR)
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