Title:
NON-HUMAN TRANSGENIC MAMMALIAN ANIMAL EXPRESSING HUMAN EGFR SPECIFICALLY IN LUNGS
Document Type and Number:
WIPO Patent Application WO/2017/026383
Kind Code:
A1
Abstract:
[Problem] To provide transgenic mice in which h-EGFR is expressed specifically in the lungs, and which spontaneously develop lung cancer. [Solution] A non-human transgenic mammalian animal (specifically, a transgenic mouse) in which a human [L858R] epidermal growth factor receptor ([L858R]-h-EGFR) gene, which includes an entire genetic region of 28 exons and 27 introns, is inserted downstream of the expression promoter region thereof. When doing so, it is preferable that the expression promoter region includes a tetracycline response element (TRE), and that the non-human transgenic mammal has a reverse tetracycline-controlled transactivator (rtTA) gene inserted downstream of the expression promoter of the lung-specific expression gene in the mammal.
Inventors:
GABAZZA ESTEBAN CESAR (JP)
GABAZZA CORINA (JP)
GABAZZA CORINA (JP)
Application Number:
PCT/JP2016/073053
Publication Date:
February 16, 2017
Filing Date:
August 05, 2016
Export Citation:
Assignee:
UNIV MIE (JP)
International Classes:
C12N15/09; A01K67/027
Foreign References:
| JP2002508960A | 2002-03-26 | |||
| JP2008212081A | 2008-09-18 |
Other References:
REGALES, LUCIA ET AL.: "Development of New Mouse Lung Tumor Models Expressing EGFR T790M Mutants Associated with Clinical Resistance to Kinase Inhibitors", PLOS ONE, vol. 2, no. Issue 8, 2007, pages e810, XP055366256
POLITI, KATERINA ET AL.: "Lung adenocarcinomas induced in mice by mutant EGF receptors found in human lung cancers respond to a tyrosine kinase inhibitor or to down-regulation of the receptors", GENES & DEVELOPMENT, vol. 20, 2006, pages 1496 - 1510, XP055366258
EICK, DIRK ET AL.: "The RNA Polymerase II Carboxy-Terminal Domain (CTD) Code, Chemical Reviews , 2013", GENE EXPRESSION, 2013, pages 8456 - 8490, XP055080576
ICHIRO MIYOSHI: "Dai 60 Kai Nagoya City University Igaku Sokai Tokubetsu Koen II, ''Development of animal models and their applications", NAGOYA MED. J., vol. 51, 2010, pages 55 - 61
DATABASE nucleotide [O] 11 April 2004 (2004-04-11), LIVINGSTON, R. J. ET AL.: "Homo sapiens epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) (EGFR) gene , complete cds", XP055366268, retrieved from ncbi Database accession no. AY588246.1
KWON, MIN-CHUL ET AL.: "Mouse models for lung cancer", MOLECULAR ONCOLOGY, vol. 7, 2013, pages 165 - 177
WALRATH, JESSICA C. ET AL.: "Genetically Engineered Mouse Models in Cancer Research", ADV CANCER RES., vol. 106, 2012, pages 113 - 164
POLITI, KATERINA ET AL.: "Lung adenocarcinomas induced in mice by mutant EGF receptors found in human lung cancers respond to a tyrosine kinase inhibitor or to down-regulation of the receptors", GENES & DEVELOPMENT, vol. 20, 2006, pages 1496 - 1510, XP055366258
EICK, DIRK ET AL.: "The RNA Polymerase II Carboxy-Terminal Domain (CTD) Code, Chemical Reviews , 2013", GENE EXPRESSION, 2013, pages 8456 - 8490, XP055080576
ICHIRO MIYOSHI: "Dai 60 Kai Nagoya City University Igaku Sokai Tokubetsu Koen II, ''Development of animal models and their applications", NAGOYA MED. J., vol. 51, 2010, pages 55 - 61
DATABASE nucleotide [O] 11 April 2004 (2004-04-11), LIVINGSTON, R. J. ET AL.: "Homo sapiens epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) (EGFR) gene , complete cds", XP055366268, retrieved from ncbi Database accession no. AY588246.1
KWON, MIN-CHUL ET AL.: "Mouse models for lung cancer", MOLECULAR ONCOLOGY, vol. 7, 2013, pages 165 - 177
WALRATH, JESSICA C. ET AL.: "Genetically Engineered Mouse Models in Cancer Research", ADV CANCER RES., vol. 106, 2012, pages 113 - 164
Attorney, Agent or Firm:
KOBAYASHI, Youhei (JP)
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