Title:
NOVEL 12 ALPHA-HYDROXYSTEROID DEHYDROGENASES, PRODUCTION AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2009/118176
Kind Code:
A8
Abstract:
The invention relates to 12 α-hydroxysteroid dehydrogenases, nucleic acid sequences coding for the same, expression cassettes and vectors, recombinant microorganisms containing the corresponding coding nucleic acid sequences, a method for producing said 12 α-hydroxysteroid dehydrogenases, a method for enzymatic oxidation of 12 α-hydroxysteroids using said enzyme, a method for enzymatic reduction of 12-ketosteroids using said enzyme, a method for qualitative or quantitative determination of 12-ketosteroids and/or12α-hydroxysteroids using said 12α-hydroxysteroid dehydrogenases and a method for production of ursodesoxycholic acid, comprising the enzyme-catalysed cholic acid oxidation using said 12 α-hydroxysteroid dehydrogenases.
Inventors:
AIGNER ARNO (DE)
GROSS RALF (DE)
SCHMID ROLF (DE)
BRAUN MICHAEL (DE)
MAUER STEFFEN (DE)
GROSS RALF (DE)
SCHMID ROLF (DE)
BRAUN MICHAEL (DE)
MAUER STEFFEN (DE)
Application Number:
PCT/EP2009/002190
Publication Date:
October 28, 2010
Filing Date:
March 25, 2009
Export Citation:
Assignee:
PHARMAZELL GMBH (DE)
AIGNER ARNO (DE)
GROSS RALF (DE)
SCHMID ROLF (DE)
BRAUN MICHAEL (DE)
MAUER STEFFEN (DE)
AIGNER ARNO (DE)
GROSS RALF (DE)
SCHMID ROLF (DE)
BRAUN MICHAEL (DE)
MAUER STEFFEN (DE)
International Classes:
C12N9/04
Attorney, Agent or Firm:
SCHWEIGER, Georg (Kinzebach & Partner Sternwartstrasse 4, GbR München, DE)
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