Title:
NOVEL CORONAVIRUS RECOMBINANT PROTEIN SUBUNIT VACCINE
Document Type and Number:
WIPO Patent Application WO/2022/134487
Kind Code:
A1
Abstract:
A fusion protein of a polypeptide and a Helicobacter pylori ferritin, and a subunit vaccine for preventing novel coronavirus (SARS-CoV-2) infection prepared by using the fusion protein. The polypeptide comprises one or more S1 proteins or fragments thereof (SS0, SS1, SS2, or SS3 protein, or a mutant fragment SS1t of the SS1 protein), and the amino acid sequences thereof are derived from the sequences of the novel coronavirus S protein and are optimized. The polypeptide or fragment can be fused with the codon-optimized Helicobacter pylori ferritin by means of a linker to form a fusion protein to be expressed. The fusion protein has the advantage of being easy to purify while having a high expression level in CHO cells, and can produce a high-titer neutralizing antibody against novel coronavirus (SARS-CoV-2) after immunizing an animal, and can cover various virus strains of novel coronavirus comprising mutant strains.
Inventors:
SONG CHUNYU (CN)
XU WEIWEI (CN)
DONG RUOBEI (CN)
LAN QING (CN)
CHEN XIAOJUAN (CN)
SI HUANHUAN (CN)
XU WEIWEI (CN)
DONG RUOBEI (CN)
LAN QING (CN)
CHEN XIAOJUAN (CN)
SI HUANHUAN (CN)
Application Number:
PCT/CN2021/098951
Publication Date:
June 30, 2022
Filing Date:
June 08, 2021
Export Citation:
Assignee:
ZHEJIANG VBIOSCI INC (CN)
BEIJING VBIOSCI INC (CN)
BEIJING VBIOSCI INC (CN)
International Classes:
C07K19/00; C12N15/62
Foreign References:
| CN112552413A | 2021-03-26 | |||
| CN111560074A | 2020-08-21 | |||
| CN112076315A | 2020-12-15 |
Other References:
DATABASE PROTEIN 12 March 2021 (2021-03-12), ANONYMOUS : "Chain E, Spike protein S1", XP055945802, retrieved from NCBI Database accession no. 7BWJ_E
MA XIANCAI; ZOU FAN; YU FEI; LI RONG; YUAN YAOCHANG; ZHANG YIWEN; ZHANG XIANTAO; DENG JIEYI; CHEN TAO; SONG ZHENG; QIAO YIDAN; ZHA: "Nanoparticle Vaccines Based on the Receptor Binding Domain (RBD) and Heptad Repeat (HR) of SARS-CoV-2 Elicit Robust Protective Immune Responses", IMMUNITY, CELL PRESS, AMSTERDAM, NL, vol. 53, no. 6, 25 November 2020 (2020-11-25), AMSTERDAM, NL , pages 1315, XP086410216, ISSN: 1074-7613, DOI: 10.1016/j.immuni.2020.11.015
LI TINGTING, ET AL.: "Recent Progress and Trends in Vaccine Immunogen Research　", BULLETIN OF NATIONAL NATURAL SCIENCE FOUNDATION OF CHINA, vol. 34, no. 5, 25 October 2020 (2020-10-25), pages 554 - 564, XP055945796, ISSN: 1000-8217, DOI: 10.16262/j.cnki.1000-8217.2020.05.005
MA XIANCAI; ZOU FAN; YU FEI; LI RONG; YUAN YAOCHANG; ZHANG YIWEN; ZHANG XIANTAO; DENG JIEYI; CHEN TAO; SONG ZHENG; QIAO YIDAN; ZHA: "Nanoparticle Vaccines Based on the Receptor Binding Domain (RBD) and Heptad Repeat (HR) of SARS-CoV-2 Elicit Robust Protective Immune Responses", IMMUNITY, CELL PRESS, AMSTERDAM, NL, vol. 53, no. 6, 25 November 2020 (2020-11-25), AMSTERDAM, NL , pages 1315, XP086410216, ISSN: 1074-7613, DOI: 10.1016/j.immuni.2020.11.015
LI TINGTING, ET AL.: "Recent Progress and Trends in Vaccine Immunogen Research　", BULLETIN OF NATIONAL NATURAL SCIENCE FOUNDATION OF CHINA, vol. 34, no. 5, 25 October 2020 (2020-10-25), pages 554 - 564, XP055945796, ISSN: 1000-8217, DOI: 10.16262/j.cnki.1000-8217.2020.05.005
Attorney, Agent or Firm:
CHINA SCIENCE PATENT & TRADEMARK AGENT LTD. (CN)
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