Title:
A NOVEL EMBRYO SAC SPECIFIC BIDIRECTIONAL PROMOTER FROM ARABIDOPSIS
Document Type and Number:
WIPO Patent Application WO/2016/092570
Kind Code:
A3
Abstract:
The Arabidopsis thaliana promoter trap line GUS-650 showing uidA reporter gene expression specifically in mature embryo sacs was found to carry T-DNA insertion in the 5' UTR of At3g17150. In the GUS-650 line, uidA reporter gene was inserted in head-to-head orientation to the At3g17140 gene. Presence of At3g17140 transcripts in the GUS-650 line indicated that T-DNA insertion did not affect this gene and the 668 bp intergenic region functions as a bi-directional promoter. The 672 bp fragment separating the uidA and At3g17140 genes was cloned and placed in forward or reverse orientation next to the uidA gene. Transgenic Arabidopsis plants carrying either of these constructs showed GUS activity in the ovules confirming that the 672 bp sequence is a bi-directional promoter specific to ovules. Being specific to ovules and developing seeds, this promoter could be employed in improving nutritional quality of seeds through engineering of metabolic pathways. Further, ability of this promoter to drive gene expression in the embryo sac during early stages of differentiation could be deployed to manipulate reproductive pathways in plants.
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Inventors:
SREENIVASULU YELAM (IN)
SHARMA ISHA (IN)
SRINIVASAN RAMAMURTHY (IN)
RAMACHANDRA BHAT SHRIPAD (IN)
SINGH AHUJA PARAMVIR (IN)
SHARMA ISHA (IN)
SRINIVASAN RAMAMURTHY (IN)
RAMACHANDRA BHAT SHRIPAD (IN)
SINGH AHUJA PARAMVIR (IN)
Application Number:
PCT/IN2015/050197
Publication Date:
September 01, 2016
Filing Date:
December 10, 2015
Export Citation:
Assignee:
COUNCIL SCIENT IND RES (IN)
International Classes:
A01H5/00; C12N15/82
Domestic Patent References:
| WO2012007919A2 | 2012-01-19 |
Foreign References:
| US20110191912A1 | 2011-08-04 | |||
| US6388170B1 | 2002-05-14 |
Other References:
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SHARMA ISHA ET AL: "Identification and Characterization of a T-DNA Promoter Trap Line ofArabidopsis thalianaUncovers an Embryo Sac-Specific Bi-directional Promoter", PLANT MOLECULAR BIOLOGY REPORTER, SPRINGER SCIENCE+BUSINESS MEDIA B.V, NL, vol. 33, no. 5, 17 December 2014 (2014-12-17), pages 1404 - 1412, XP035531502, ISSN: 0735-9640, [retrieved on 20141217], DOI: 10.1007/S11105-014-0829-7
H.-J. YU: "Analysis of the Female Gametophyte Transcriptome of Arabidopsis by Comparative Expression Profiling", PLANT PHYSIOLOGY., vol. 139, no. 4, 18 November 2005 (2005-11-18), US, pages 1853 - 1869, XP055286686, ISSN: 0032-0889, DOI: 10.1104/pp.105.067314
SOFIA KOURMPETLI ET AL: "Bidirectional promoters in seed development and related hormone/stress responses", BMC PLANT BIOLOGY, BIOMED CENTRAL, LONDON, GB, vol. 13, no. 1, 22 November 2013 (2013-11-22), pages 187, XP021169273, ISSN: 1471-2229, DOI: 10.1186/1471-2229-13-187
KEDDIE J S ET AL: "A SEED-SPECIFIC BRASSICA NAPUS OLEOSIN PROMOTER INTERACTS WITH A G-BOX-SPECIFIC PROTEIN AND MAY BE BI-DIRECTIONAL", PLANT MOLECULAR BIOLOGY, SPRINGER, DORDRECHT, NL, vol. 24, no. 2, 1994, pages 327 - 340, XP001007601, ISSN: 0167-4412, DOI: 10.1007/BF00020171
RAJESH MEHROTRA ET AL: "Designer promoter: an artwork of cis engineering", PLANT MOLECULAR BIOLOGY, vol. 75, no. 6, 14 February 2011 (2011-02-14), NL, pages 527 - 536, XP055234157, ISSN: 0167-4412, DOI: 10.1007/s11103-011-9755-3
LUO M ET AL: "EXPRESSION AND PARENT-OF-ORIGIN EFFECTS OF FIS2, MEA, AND FIE IN THE ENDOSPERM AND EMBRYO OF DEVELOPING ARABIDOPSIS SEEDS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, US, vol. 97, no. 19, 29 August 2000 (2000-08-29), pages 10637 - 10642, XP001153678, ISSN: 0027-8424, DOI: 10.1073/PNAS.170292997
WANG QUAN ET AL: "Searching for bidirectional promoters in Arabidopsis thaliana", BMC BIOINFORMATICS, BIOMED CENTRAL, LONDON, GB, vol. 10, no. Suppl 1, 30 January 2009 (2009-01-30), pages S29, XP021047388, ISSN: 1471-2105, DOI: 10.1186/1471-2105-10-S1-S29
SHARMA ISHA ET AL: "Identification and Characterization of a T-DNA Promoter Trap Line ofArabidopsis thalianaUncovers an Embryo Sac-Specific Bi-directional Promoter", PLANT MOLECULAR BIOLOGY REPORTER, SPRINGER SCIENCE+BUSINESS MEDIA B.V, NL, vol. 33, no. 5, 17 December 2014 (2014-12-17), pages 1404 - 1412, XP035531502, ISSN: 0735-9640, [retrieved on 20141217], DOI: 10.1007/S11105-014-0829-7
Attorney, Agent or Firm:
PHILLIPS, Prashant et al. (B6/10 Safdarjung Enclave, New Delhi 9, IN)
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