Title:
NOVEL ENZYME PROTEIN, PROCESS FOR PRODUCTION OF THE ENZYME PROTEIN, AND GENE ENCODING THE ENZYME PROTEIN
Document Type and Number:
WIPO Patent Application WO/2012/014980
Kind Code:
A1
Abstract:
The present invention relates to: a protein which does not have a neuraminidase activity substantially and has a β-galactoside-α2,3-sialyltransferase activity; a nucleic acid which encodes the enzyme protein; and a process for producing the enzyme using a microorganism transformed with a gene encoding the protein. This protein is a modified enzyme protein produced by mutating a specific amino acid residue in a β-galactoside-α2,3-sialyltransferase protein derived from a microorganism belonging to the family Vibrio, the genus Photobacterium to substantially inactive the neuraminidase activity of the protein.
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Inventors:
MINE TOSHIKI (JP)
YAMAMOTO TAKESHI (JP)
KATAYAMA SAKURAKO (JP)
YAMAMOTO TAKESHI (JP)
KATAYAMA SAKURAKO (JP)
Application Number:
PCT/JP2011/067265
Publication Date:
February 02, 2012
Filing Date:
July 28, 2011
Export Citation:
Assignee:
JAPAN TOBACCO INC (JP)
MINE TOSHIKI (JP)
YAMAMOTO TAKESHI (JP)
KATAYAMA SAKURAKO (JP)
MINE TOSHIKI (JP)
YAMAMOTO TAKESHI (JP)
KATAYAMA SAKURAKO (JP)
International Classes:
C12N15/09; C12N9/10
Domestic Patent References:
| WO2006043406A1 | 2006-04-27 | |||
| WO2006112040A1 | 2006-10-26 | |||
| WO1998038315A1 | 1998-09-03 | |||
| WO2006112253A1 | 2006-10-26 |
Foreign References:
| US6255094B1 | 2001-07-03 |
Other References:
TSUKAMOTO H ET AL.: "Purification, cloning, and expression of an alpha/beta-galactoside alpha- 2,3-sialyltransferase from a luminous marine bacterium, Photobacterium phosphoreum", J. BIOL. CHEM., vol. 282, no. 41, 2007, pages 29794 - 29802, XP002540659
IWATANI T ET AL.: "Crystal structure of alpha/ beta-galactoside alpha2,3-sialyltransferase from a luminous marine bacterium, Photobacterium phosphoreum", FEBS LETT., vol. 583, no. 12, 2009, pages 2083 - 2087, XP026185383
MINE T ET AL.: "An alpha2,6-sialyltransferase cloned from Photobacterium leiognathi strain JT-SHIZ-119 shows both sialyltransferase and neuraminidase activity", GLYCOBIOLOGY, vol. 20, no. 2, February 2010 (2010-02-01), pages 158 - 165, XP008148076
KAJIWARA H ET AL.: "Sialyltransferases obtained from marine bacteria", J. APPL. GLYCOSCI., vol. 56, no. 2, 2009, pages 77 - 82, XP022356996
YAMAMOTO T ET AL.: "Conserved amino acid sequences in the bacterial sialyltransferases belonging to Glycosyltransferase family 80", BIOCHEM. BIOPHYS. RES. COMMUN., vol. 365, no. 2, 2008, pages 340 - 343, XP022356996, DOI: doi:10.1016/j.bbrc.2007.10.201
YAMAMOTO T ET AL.: "Bacterial sialyltransferases", TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY, vol. 18, no. 102, 2006, pages 253 - 265
TSUKAMOTO H ET AL.: "Photobacterium sp. JT-ISH- 224 produces two sialyltransferases, alpha-/ beta-galactoside alpha2,3-sialyltransferase and beta-galactoside alpha2,6-sialyltransferase", J. BIOCHEM., vol. 143, no. 2, 2008, pages 187 - 197, XP002540660
HAMAMOTO, T. ET AL., BIOORG. MED. CHEM., vol. 1, 1993, pages 141 - 145
WEINSTEIN, J. ET AL., J. BIOL. CHEM., vol. 262, 1987, pages 17735 - 17743
YAMAMOTO, T. ET AL., J. BIOCHEM., vol. 120, 1996, pages 104 - 110
TSUKAMOTO, H. ET AL., J. BIOL. CHEM., vol. 282, 2007, pages 29794 - 29802
TAKAKURA, Y. ET AL., J. BIOCHEM., (TOKYO, vol. 142, 2007, pages 403 - 412
TAKAKURA, Y. ET AL., J. BIOCHEM., vol. 142, 2007, pages 403 - 412
NUCLEIC ACID RESEARCH, vol. 10, no. 20, 1982, pages 6487 - 6500
NEEDLEMAN, S. B.; WUNSCH, C. D., J. MOL. BIOL., vol. 48, 1970, pages 443 - 453
HENIKOFF, S.; HENIKOFF, J. G., PROC. NATL. ACAD. SCI. USA, vol. 89, 1992, pages 10915 - 10919
ALTSCHUL ET AL., NUCL. ACIDS. RES., vol. 25, 1997, pages 3389 - 3402
DEVEREUX ET AL., NUCL. ACIDS RES., vol. 12, 1984, pages 387
GRIBSKOV; BURGESS, NUCL. ACIDS RES., vol. 14, 1986, pages 6745
"Atlas of Polypeptide Sequence and Structure", 1979, NATIONAL BIOMEDICAL RESEARCH FOUNDATION, pages: 353 - 358
WOOTTON; FEDERHEN, COMPUTERS AND CHEMISTRY, 1993
WOOTTON; FEDERHEN: "Analysis of compositionally biased regions in sequence databases", METHODS ENZYMOL., vol. 266, 1996, pages 544 - 71
CLAVERIE; STATES, COMPUTERS AND CHEMISTRY, 1993
SAMBROOK ET AL.: "Molecular Cloning: A Laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY PRESS
J. BIOCHEM., vol. 120, 1996, pages 104 - 110
HIGUCHI ET AL., NUCLEIC ACID RES., vol. 16, 1988, pages 7351 - 7367
HO ET AL., GENE, vol. 77, 1989, pages 51 - 59
YAMAMOTO ET AL., BBRC, vol. 365, 2008, pages 340 - 343
POUWELS ET AL.: "Cloning Vectors: A Laboratory Manual", 1985, ELSEVIER
CHANG ET AL., NATURE, vol. 275, 1978, pages 615
GOEDDEL ET AL., NATURE, vol. 281, 1979, pages 544
HINNEN ET AL., PROC. NATL. ACAD. SCI. USA, vol. 75, 1978, pages 1929 - 1933
OKAYAMA; BERG, MOL. CELL. BIOL., vol. 3, 1983, pages 280
SAMBROOK ET AL.: "Molecular Cloning, A laboratory manual", 1989
IWATANI T ET AL.: "Crystal structure of alpha/ beta-galactoside alpha2,3-sialyltransferase from a luminous marine bacterium, Photobacterium phosphoreum", FEBS LETT., vol. 583, no. 12, 2009, pages 2083 - 2087, XP026185383
MINE T ET AL.: "An alpha2,6-sialyltransferase cloned from Photobacterium leiognathi strain JT-SHIZ-119 shows both sialyltransferase and neuraminidase activity", GLYCOBIOLOGY, vol. 20, no. 2, February 2010 (2010-02-01), pages 158 - 165, XP008148076
KAJIWARA H ET AL.: "Sialyltransferases obtained from marine bacteria", J. APPL. GLYCOSCI., vol. 56, no. 2, 2009, pages 77 - 82, XP022356996
YAMAMOTO T ET AL.: "Conserved amino acid sequences in the bacterial sialyltransferases belonging to Glycosyltransferase family 80", BIOCHEM. BIOPHYS. RES. COMMUN., vol. 365, no. 2, 2008, pages 340 - 343, XP022356996, DOI: doi:10.1016/j.bbrc.2007.10.201
YAMAMOTO T ET AL.: "Bacterial sialyltransferases", TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY, vol. 18, no. 102, 2006, pages 253 - 265
TSUKAMOTO H ET AL.: "Photobacterium sp. JT-ISH- 224 produces two sialyltransferases, alpha-/ beta-galactoside alpha2,3-sialyltransferase and beta-galactoside alpha2,6-sialyltransferase", J. BIOCHEM., vol. 143, no. 2, 2008, pages 187 - 197, XP002540660
HAMAMOTO, T. ET AL., BIOORG. MED. CHEM., vol. 1, 1993, pages 141 - 145
WEINSTEIN, J. ET AL., J. BIOL. CHEM., vol. 262, 1987, pages 17735 - 17743
YAMAMOTO, T. ET AL., J. BIOCHEM., vol. 120, 1996, pages 104 - 110
TSUKAMOTO, H. ET AL., J. BIOL. CHEM., vol. 282, 2007, pages 29794 - 29802
TAKAKURA, Y. ET AL., J. BIOCHEM., (TOKYO, vol. 142, 2007, pages 403 - 412
TAKAKURA, Y. ET AL., J. BIOCHEM., vol. 142, 2007, pages 403 - 412
NUCLEIC ACID RESEARCH, vol. 10, no. 20, 1982, pages 6487 - 6500
NEEDLEMAN, S. B.; WUNSCH, C. D., J. MOL. BIOL., vol. 48, 1970, pages 443 - 453
HENIKOFF, S.; HENIKOFF, J. G., PROC. NATL. ACAD. SCI. USA, vol. 89, 1992, pages 10915 - 10919
ALTSCHUL ET AL., NUCL. ACIDS. RES., vol. 25, 1997, pages 3389 - 3402
DEVEREUX ET AL., NUCL. ACIDS RES., vol. 12, 1984, pages 387
GRIBSKOV; BURGESS, NUCL. ACIDS RES., vol. 14, 1986, pages 6745
"Atlas of Polypeptide Sequence and Structure", 1979, NATIONAL BIOMEDICAL RESEARCH FOUNDATION, pages: 353 - 358
WOOTTON; FEDERHEN, COMPUTERS AND CHEMISTRY, 1993
WOOTTON; FEDERHEN: "Analysis of compositionally biased regions in sequence databases", METHODS ENZYMOL., vol. 266, 1996, pages 544 - 71
CLAVERIE; STATES, COMPUTERS AND CHEMISTRY, 1993
SAMBROOK ET AL.: "Molecular Cloning: A Laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY PRESS
J. BIOCHEM., vol. 120, 1996, pages 104 - 110
HIGUCHI ET AL., NUCLEIC ACID RES., vol. 16, 1988, pages 7351 - 7367
HO ET AL., GENE, vol. 77, 1989, pages 51 - 59
YAMAMOTO ET AL., BBRC, vol. 365, 2008, pages 340 - 343
POUWELS ET AL.: "Cloning Vectors: A Laboratory Manual", 1985, ELSEVIER
CHANG ET AL., NATURE, vol. 275, 1978, pages 615
GOEDDEL ET AL., NATURE, vol. 281, 1979, pages 544
HINNEN ET AL., PROC. NATL. ACAD. SCI. USA, vol. 75, 1978, pages 1929 - 1933
OKAYAMA; BERG, MOL. CELL. BIOL., vol. 3, 1983, pages 280
SAMBROOK ET AL.: "Molecular Cloning, A laboratory manual", 1989
Attorney, Agent or Firm:
ONO, Shinjiro et al. (JP)
Ono Shinjiro (JP)
Ono Shinjiro (JP)
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