Title:
NOVEL FRACTIONATION METHOD FOR NUCLEAR SOLUBLE PROTEINS
Document Type and Number:
WIPO Patent Application WO/2022/118894
Kind Code:
A1
Abstract:
The present invention provides a fractionation method for nuclear soluble proteins, said method achieving a high yield and being capable of preventing intranuclear soluble proteins from leakage outside nuclei due to diffusion, and a kit for performing this fractionation method for nuclear soluble proteins. The fractionation method for nuclear soluble proteins according to the present invention involves a step for exposing cells to digitonin under a temperature condition of 10-37°C. The temperature condition may be 25-37°C. The fractionation method for nuclear soluble proteins according to the present invention can involve a step for exposing the cells to WGA. The present invention provides a kit for performing the fractionation method for nuclear soluble proteins according to the present invention, said kit containing digitonin. The kit according to the present invention can contain WGA.
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Inventors:
OGAWA YUTAKA (JP)
Application Number:
PCT/JP2021/044140
Publication Date:
June 09, 2022
Filing Date:
December 01, 2021
Export Citation:
Assignee:
RIKEN (JP)
International Classes:
C07K1/14; C12M1/26; C12N5/07; G01N33/50; G01N33/68
Foreign References:
| JP2019510224A | 2019-04-11 | |||
| JP2008511289A | 2008-04-17 | |||
| JP2020506671A | 2020-03-05 |
Other References:
MAKOTO KIMURA, KETAN THAKAR, SAMIR KARACA, NAOKO IMAMOTO, RALPH H KEHLENBACH: "Novel Approaches for the Identification of Nuclear Transport Receptor Substrates", NUCLEAR PORE COMPLEXES AND NUCLEOCYTOPLASMIC TRANSPORT - METHODS, 1 January 2014 (2014-01-01), NL, pages 353 - 378, XP009537234, ISBN: 978-0-12-417160-2, DOI: 10.1016/B978-0-12-417160-2.00016-3
MOHR DAGMAR, FREY STEFFEN, FISCHER TORSTEN, GÜ TTLER THOMAS, GÖ RLICH DIRK: "Characterisation of the passive permeability barrier of nuclear pore complexes", THE EMBO JOURNAL, vol. 28, 1 January 2009 (2009-01-01), pages 2541 - 2553, XP055937153
OGAWA Y., IMAMOTO, N.: "Methods to separate nuclear soluble fractions reflecting localizations in living cells", ISCIENCE, vol. 24, no. 12, pages 1 - 19, XP055937158
MOHR DAGMAR, FREY STEFFEN, FISCHER TORSTEN, GÜ TTLER THOMAS, GÖ RLICH DIRK: "Characterisation of the passive permeability barrier of nuclear pore complexes", THE EMBO JOURNAL, vol. 28, 1 January 2009 (2009-01-01), pages 2541 - 2553, XP055937153
OGAWA Y., IMAMOTO, N.: "Methods to separate nuclear soluble fractions reflecting localizations in living cells", ISCIENCE, vol. 24, no. 12, pages 1 - 19, XP055937158
Attorney, Agent or Firm:
TAKASHIMA, Hajime (JP)
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