Title:
NOVEL GENE CLUSTER OF PEDERIN BIOSYNTHESIS GENES
Document Type and Number:
WIPO Patent Application WO2003044186
Kind Code:
A3
Abstract:
The present invention relates to the cloning, sequencing and analysing of a gene cluster encoding a modular polyketide synthase enzyme involved in the biosynthesis of the antitumor compound pederin. This novel cluster represents the first example of genes from an unculturable symbiont encoding the biosynthesis of a drug candidate.
Inventors:
PIEL JOERN (DE)
Application Number:
PCT/EP2002/013085
Publication Date:
January 29, 2004
Filing Date:
November 21, 2002
Export Citation:
Assignee:
MAX PLANCK GESELLSCHAFT (DE)
PIEL JOERN (DE)
PIEL JOERN (DE)
International Classes:
C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/09; C12N9/00; C12N9/02; C12N9/10; C12N15/52; C12P17/06; C12P17/16; C12P19/00; (IPC1-7): C12N9/10; C12N9/02; C12N15/52; C12P19/00
Other References:
KELLNER RUPERT L L: "Suppression of pederin biosynthesis through antibiotic elimination of endosymbionts in Paederus sabaeus.", JOURNAL OF INSECT PHYSIOLOGY, vol. 47, no. 4-5, April 2001 (2001-04-01), pages 475 - 483, XP002235145, ISSN: 0022-1910
CARDANI C ET AL: "THE BIOSYNTHESIS OF PEDERIN", TETRAHEDRON LETTERS, vol. 30., 1973, pages 2815 - 2818, XP001147015, ISSN: 0040-4039
BEYER S ET AL: "Metabolic diversity in myxobacteria: identification of the myxalamid and the stigmatellin biosynthetic gene cluster of Stigmatella aurantiaca Sg a15 and a combined polyketide-(poly)peptide gene cluster from the epothilone producing strain Sorangium cellulosum So ce90", BIOCHIMICA ET BIOPHYSICA ACTA. GENE STRUCTURE AND EXPRESSION, ELSEVIER, AMSTERDAM, NL, vol. 1445, no. 2, 14 May 1999 (1999-05-14), pages 185 - 195, XP004275376, ISSN: 0167-4781
DATABASE EMBL [online] 2 April 2001 (2001-04-02), "Pseudomonas fluorescens NCIMB 10586 mupirocin biosynthetic gene cluster", XP002235150, Database accession no. AF318063
NARQUIZIAN R ET AL: "The pederin family of antitumor agents: structures, synthesis and biological activity.", ERNST SCHERING RESEARCH FOUNDATION WORKSHOP. GERMANY 2000, no. 32, 2000, pages 25 - 56, XP001145840, ISSN: 0947-6075
RODRIGUEZ EDUARDO ET AL: "Combinatorial biosynthesis of antimicrobials and other natural products.", CURRENT OPINION IN MICROBIOLOGY, vol. 4, no. 5, October 2001 (2001-10-01), pages 526 - 534, XP002235147, ISSN: 1369-5274
CANE DAVID E ET AL: "The parallel and convergent universes of polyketide synthases and nonribosomal peptide synthetases.", CHEMISTRY & BIOLOGY (LONDON), vol. 6, no. 12, December 1999 (1999-12-01), pages R319 - R325, XP002235148, ISSN: 1074-5521
PIEL JOERN: "A polyketide synthase-peptide synthetase gene cluster from an uncultured bacterial symbiont of Paederus beetles.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 99, no. 22, 29 October 2002 (2002-10-29), October 29 2002 2002, pages 14002 - 14007, XP002235149, ISSN: 0027-8424
CARDANI C ET AL: "THE BIOSYNTHESIS OF PEDERIN", TETRAHEDRON LETTERS, vol. 30., 1973, pages 2815 - 2818, XP001147015, ISSN: 0040-4039
BEYER S ET AL: "Metabolic diversity in myxobacteria: identification of the myxalamid and the stigmatellin biosynthetic gene cluster of Stigmatella aurantiaca Sg a15 and a combined polyketide-(poly)peptide gene cluster from the epothilone producing strain Sorangium cellulosum So ce90", BIOCHIMICA ET BIOPHYSICA ACTA. GENE STRUCTURE AND EXPRESSION, ELSEVIER, AMSTERDAM, NL, vol. 1445, no. 2, 14 May 1999 (1999-05-14), pages 185 - 195, XP004275376, ISSN: 0167-4781
DATABASE EMBL [online] 2 April 2001 (2001-04-02), "Pseudomonas fluorescens NCIMB 10586 mupirocin biosynthetic gene cluster", XP002235150, Database accession no. AF318063
NARQUIZIAN R ET AL: "The pederin family of antitumor agents: structures, synthesis and biological activity.", ERNST SCHERING RESEARCH FOUNDATION WORKSHOP. GERMANY 2000, no. 32, 2000, pages 25 - 56, XP001145840, ISSN: 0947-6075
RODRIGUEZ EDUARDO ET AL: "Combinatorial biosynthesis of antimicrobials and other natural products.", CURRENT OPINION IN MICROBIOLOGY, vol. 4, no. 5, October 2001 (2001-10-01), pages 526 - 534, XP002235147, ISSN: 1369-5274
CANE DAVID E ET AL: "The parallel and convergent universes of polyketide synthases and nonribosomal peptide synthetases.", CHEMISTRY & BIOLOGY (LONDON), vol. 6, no. 12, December 1999 (1999-12-01), pages R319 - R325, XP002235148, ISSN: 1074-5521
PIEL JOERN: "A polyketide synthase-peptide synthetase gene cluster from an uncultured bacterial symbiont of Paederus beetles.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 99, no. 22, 29 October 2002 (2002-10-29), October 29 2002 2002, pages 14002 - 14007, XP002235149, ISSN: 0027-8424
Download PDF:
Previous Patent: PURIFIED POLYPEPTIDES INVOLVED IN GENERAL METABOLISM
Next Patent: EMULSION COMPOSITIONS
Next Patent: EMULSION COMPOSITIONS