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Title:
NOVEL L-RHAMNOSE ISOMERASE
Document Type and Number:
WIPO Patent Application WO/2022/114207
Kind Code:
A1
Abstract:
[Problem] To provide a L-rhamnose isomerase that is highly safe, can be used in the food industry, and has high thermostability, and to provide a microorganism for producing the same and a manufacturing method for an aldose or a ketose. [Solution] A L-rhamnose isomerase derived from a microorganism belonging to the genus Enterobacter, said L-rhamnose isomerase having a subunit molecular mass of 47 kDa that is measured using SDS-PAGE, and having substrate specificities (A) and (B).　(A) Having an isomerase activity that recognizes a CHO group at C1 and an OH group at C2 in an aldose, reacts, and converts the CHO group at C1 into an OH group and the OH group at C2 into a CO group, or that recognizes an OH group at C1 and a CO group at C2 of a ketose, reacts, and converts the OH group at C1 into a CHO group and the CO group at C2 into an OH group.　(B) Having reactivity with L-rhamnose, L-lyxose, L-mannose, D-allose, D-ribose, and L-talose.

Inventors:
AKIMITSU KAZUYA (JP)
IZUMORI KEN (JP)
YOSHIHARA AKIHIDE (JP)
KATO SHIRO (JP)
MOCHIZUKI SUSUMU (JP)
YOSHIDA HIROMI (JP)
KAMITORI SHIGEHIRO (JP)
Application Number:
PCT/JP2021/043758
Publication Date:
June 02, 2022
Filing Date:
November 30, 2021
Export Citation:
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Assignee:
UNIV KAGAWA NAT UNIV CORP (JP)
International Classes:
C12N15/61; C12N1/15; C12N1/19; C12N1/20; C12N1/21; C12N5/10; C12N9/90; C12N11/00; C12N11/08; C12N15/31; C12N15/63; C12P19/02
Domestic Patent References:
WO2021193949A12021-09-30
WO2006022239A12006-03-02
WO2020189000A12020-09-24
WO2020195037A12020-10-01
Foreign References:
JP2004298105A2004-10-28
JP5330976B22013-10-30
JP5317055B22013-10-16
JP5158779B22013-03-06
JP4943839B22012-05-30
JP4724824B22011-07-13
JP2009269887A2009-11-19
Other References:
DATABASE Uniprot Uniprot; . . : "Full=L-rhamnose isomerase {ECO:0000313|EMBL:MXG74132.1}; DE EC=5.3.1.14 {ECO:0000313|EMBL:MXG74132.1}", XP055933635
DATABASE Uniprot Uniprot; . . : "Full=L-rhamnose isomerase {ECO:0000313|EMBL:TCB89324.1}; DE EC=5.3.1.14 {ECO:0000313|EMBL:TCB89324.1}", XP055933639, Database accession no. A0A4R0FYS9_9ENTR
DATABASE Uniprot Uniprot; . . : ": Full=L-rhamnose isomerase {ECO:0000256|HAMAP-Rule:MF_00541}; DE EC=5.3.1.14 {ECO:0000256|HAMAP-Rule:MF_00541}", XP055933797, Database accession no. A0A431SFU6_9ENTR
DATABASE Uniprot Uniprot; . . : " Full=L-rhamnose isomerase {ECO:0000256|HAMAP-Rule:MF_00541}; DE EC=5.3.1.14 {ECO:0000256|HAMAP-Rule:MF_00541}", XP055933802, Database accession no. A0A263VPB6_ENTAS
DATABASE UniProt UniProt; . . : "Full=L-rhamnose isomerase {ECO:0000256|HAMAP-Rule:MF_00541}; DE EC=5.3.1.14 {ECO:0000256|HAMAP-Rule:MF_00541}", XP055933805, Database accession no. A0A495LJ50_ENTAS
DATABASE UniProt UniProt; . . : "Full=L-rhamnose isomerase {ECO:0000256|HAMAP-Rule:MF_00541}; DE EC=5.3.1.14 {ECO:0000256|HAMAP-Rule:MF_00541}", XP055933808, Database accession no. A0A428LFP8_9ENTR
J. FERMENT. BIOENG., vol. 84, 1997, pages 319
KARLINALTSCHUL, PROC. NATL. ACAD. SCI. USA, vol. 87, 1990, pages 2264 - 68
KARLINALTSCHUL, PROC. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 5873 - 7
ALTSCHUL ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 10
YODOSHA ET AL.: "PCR method or annealing method", article "New Handbook of Genetic Engineering", pages: 82 - 88
Attorney, Agent or Firm:
SUDO, Akinobu et al. (JP)
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