Title:
NOVEL LIPASE CAPABLE OF DECOMPOSING OIL OR FAT CONTAINING TRANS-FATTY ACID
Document Type and Number:
WIPO Patent Application WO/2020/009231
Kind Code:
A1
Abstract:
The present disclosure provides a technique for decomposing an oil or fat containing a trans-fatty acid. A novel lipase according to the present disclosure has an activity to decompose an oil or fat containing a trans-fatty acid. The lipase according to the present disclosure comprises a polypeptide comprising an amino acid sequence represented by SEQ ID NO: 4, 11, 16 or 18, or a polypeptide encoded by a nucleotide sequence represented by SEQ ID NO: 1, 7, 15 or 17, or a polypeptide having at least 70% sequence homology with the above-mentioned polypeptides. The lipase has an ability to decompose an oil or fat even at a high temperature, and is found to have excellent thermal stability. The lipase according to the present disclosure is useful for an oil treatment, such as the treatment of wastewater, a detergent, and a technique for modifying a fat or for producing an oil or fat.
Inventors:
HORI KATSUTOSHI (JP)
Application Number:
PCT/JP2019/026848
Publication Date:
January 09, 2020
Filing Date:
July 05, 2019
Export Citation:
Assignee:
UNIV NAGOYA NAT UNIV CORP (JP)
International Classes:
C12N15/55; C02F1/00; C02F3/34; C11D3/386; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/20
Foreign References:
| JP2008086277A | 2008-04-17 | |||
| JP2010227858A | 2010-10-14 | |||
| JP2018129504A | 2018-08-16 |
Other References:
TAKEDA, Y. ET AL.: "Purification, characterization, and molecular cloning of organic- solvent-tolerant cholesterol esterase from cyclohexane-tolerant Burkholderia cepacia strain ST-200", EXTREMOPHILES, vol. 10, no. 4, 7 February 2006 (2006-02-07), pages 269 - 277, XP019431284, DOI: 10.1007/s00792-005-0494-8
LU , Y. ET AL.: "Identification of bacteria producing a thermophilic lipase with positional non-specificity and characterization of the lipase", ANNALS OF MICROBIOLOGY, vol. 59, no. 3, 2009, pages 565 - 571, XP055673920, DOI: 10.1007/BF03175147
DATABASE Protein [online] 8 January 2016 (2016-01-08), "triacylglycerol lipase [Burkholderia cepacia JBK9", XP055673925, retrieved from NCBI Database accession no. ALX14739
DATABASE Protein [online] 29 October 2016 (2016-10-29), "triacylglycerol lipase [Burkholderia sp. NFACC38- 1]", XP055673929, retrieved from NCBI Database accession no. SET69416
DATABASE Protein [online] 22 January 2016 (2016-01-22), "triacylglycerol lipase [Burkholderia cepacia]", XP055673932, retrieved from NCBI Database accession no. KVK76798.1
MATSUOKA, H. ET AL.: "Symbiotic effects of a lipase-secreting bacterium, Burkholderia arboris SL1B1, and a glycerol-assimilating yeast, Candida cylindracea SL1B2, on triacylglycerol degradation", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 107, no. 4, April 2009 (2009-04-01), pages 401 - 408, XP026077569, DOI: 10.1016/j.jbiosc.2008.12.001
MARYAM TAKENORI : "Quantification of trans fatty acid", FOOD INDUSTRY FOR TOMORROW, vol. 425, 2012, pages 11 - 16, XP009525458, ISSN: 0385-5864
BORGDORF, R.; MUKHERJEE, K.D.; WARWEL, S.: "Anwendung lipasekatalysierter Reaktionen bei der Herstellung spezieller cis- und trans-Octadecensauren", BIOKONVERSION NACHWACHSENDER ROHSTOFFE : WORKSHOP AM 30.06. UND 01.07.1997 IN DETMOLD, vol. 10, 1998, pages 197 - 202, XP009525709, ISBN: 3-7843-2926-8
BATZER ET AL., NUCLEIC ACID RES., vol. 19, 1991, pages 5081
OHTSUKA ET AL., J. BIOL. CHEM., vol. 260, 1985, pages 2605 - 2608
ROSSOLINI ET AL., MOL. CELL. PROBES, vol. 8, 1994, pages 91 - 98
MARK ET AL., PROC NATL ACAD SCI USA., vol. 81, no. 18, September 1984 (1984-09-01), pages 5662 - 5666
ZOLLER ET AL., NUCLEIC ACIDS RES, vol. 10, no. 20, 25 October 1982 (1982-10-25), pages 6487 - 6500
WANG ET AL., SCIENCE, vol. 224, no. 4656, 29 June 1984 (1984-06-29), pages 1431 - 1433
AUSUBEL ET AL.: "DNA Cloning 1: Core Techniques, A Practical Approach", 1995, WILEY INTERSCIENCE PUBLISHERS
SAMBROOK ET AL.: "Molecular Cloning: A Laboratory Manual", vol. 1, 2001, COLD SPRING HARBOR LABORATORY PRESS, pages: 42 - 45
ALTSCHUL ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 410
PEARSONLIPMAN, PROC. NATL. ACAD. SCI., USA, vol. 85, 1988, pages 2444 - 2448
SMITHWATERMAN, J. MOL. BIOL., vol. 147, 1981, pages 195 - 197
NEEDLEMANWUNSCH, J. MOL. BIOL., vol. 48, 1970, pages 443 - 453
SAVLI, H.KARADENIZLI, A.KOLAYLI, F.GUNDES, S.OZBEK, U.VAHABOGLU, H.: "Expression stability of six housekeeping genes: A proposal for resistance gene quantification studies of Pseudomonas aeruginosa by real-time quantitative RT-PCR", J. MED. MICROBIOL., vol. 52, 2003, pages 403 - 408
MARIE-ANGE TESTEMANON DUQUENNEJEAN M FRANCOISJEAN-LUC PARROU: "Validation of reference genes for quantitative expression analysis by real-time RT-PCR in Saccharomyces cerevisiae", BMC MOLECULAR BIOLOGY, vol. 10, 2009, pages 99, XP021062364, DOI: 10.1186/1471-2199-10-99
SEIJ I ISHIIHIROSHI OKUMURACHIYO MATSUBARAFUMI NINOMIYAHIROSHI YOSHIOKA: "Netsukannousei Polima wo Mochiita Suichuyubun no Kani Sokutei Hoho [Simple method of measuring oil-in-water content using heat sensitive polymer", JOURNAL OF WATER AND WASTE, vol. 46, no. 12, 2004
See also references of EP 3819379A4
LU , Y. ET AL.: "Identification of bacteria producing a thermophilic lipase with positional non-specificity and characterization of the lipase", ANNALS OF MICROBIOLOGY, vol. 59, no. 3, 2009, pages 565 - 571, XP055673920, DOI: 10.1007/BF03175147
DATABASE Protein [online] 8 January 2016 (2016-01-08), "triacylglycerol lipase [Burkholderia cepacia JBK9", XP055673925, retrieved from NCBI Database accession no. ALX14739
DATABASE Protein [online] 29 October 2016 (2016-10-29), "triacylglycerol lipase [Burkholderia sp. NFACC38- 1]", XP055673929, retrieved from NCBI Database accession no. SET69416
DATABASE Protein [online] 22 January 2016 (2016-01-22), "triacylglycerol lipase [Burkholderia cepacia]", XP055673932, retrieved from NCBI Database accession no. KVK76798.1
MATSUOKA, H. ET AL.: "Symbiotic effects of a lipase-secreting bacterium, Burkholderia arboris SL1B1, and a glycerol-assimilating yeast, Candida cylindracea SL1B2, on triacylglycerol degradation", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 107, no. 4, April 2009 (2009-04-01), pages 401 - 408, XP026077569, DOI: 10.1016/j.jbiosc.2008.12.001
MARYAM TAKENORI : "Quantification of trans fatty acid", FOOD INDUSTRY FOR TOMORROW, vol. 425, 2012, pages 11 - 16, XP009525458, ISSN: 0385-5864
BORGDORF, R.; MUKHERJEE, K.D.; WARWEL, S.: "Anwendung lipasekatalysierter Reaktionen bei der Herstellung spezieller cis- und trans-Octadecensauren", BIOKONVERSION NACHWACHSENDER ROHSTOFFE : WORKSHOP AM 30.06. UND 01.07.1997 IN DETMOLD, vol. 10, 1998, pages 197 - 202, XP009525709, ISBN: 3-7843-2926-8
BATZER ET AL., NUCLEIC ACID RES., vol. 19, 1991, pages 5081
OHTSUKA ET AL., J. BIOL. CHEM., vol. 260, 1985, pages 2605 - 2608
ROSSOLINI ET AL., MOL. CELL. PROBES, vol. 8, 1994, pages 91 - 98
MARK ET AL., PROC NATL ACAD SCI USA., vol. 81, no. 18, September 1984 (1984-09-01), pages 5662 - 5666
ZOLLER ET AL., NUCLEIC ACIDS RES, vol. 10, no. 20, 25 October 1982 (1982-10-25), pages 6487 - 6500
WANG ET AL., SCIENCE, vol. 224, no. 4656, 29 June 1984 (1984-06-29), pages 1431 - 1433
AUSUBEL ET AL.: "DNA Cloning 1: Core Techniques, A Practical Approach", 1995, WILEY INTERSCIENCE PUBLISHERS
SAMBROOK ET AL.: "Molecular Cloning: A Laboratory Manual", vol. 1, 2001, COLD SPRING HARBOR LABORATORY PRESS, pages: 42 - 45
ALTSCHUL ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 410
PEARSONLIPMAN, PROC. NATL. ACAD. SCI., USA, vol. 85, 1988, pages 2444 - 2448
SMITHWATERMAN, J. MOL. BIOL., vol. 147, 1981, pages 195 - 197
NEEDLEMANWUNSCH, J. MOL. BIOL., vol. 48, 1970, pages 443 - 453
SAVLI, H.KARADENIZLI, A.KOLAYLI, F.GUNDES, S.OZBEK, U.VAHABOGLU, H.: "Expression stability of six housekeeping genes: A proposal for resistance gene quantification studies of Pseudomonas aeruginosa by real-time quantitative RT-PCR", J. MED. MICROBIOL., vol. 52, 2003, pages 403 - 408
MARIE-ANGE TESTEMANON DUQUENNEJEAN M FRANCOISJEAN-LUC PARROU: "Validation of reference genes for quantitative expression analysis by real-time RT-PCR in Saccharomyces cerevisiae", BMC MOLECULAR BIOLOGY, vol. 10, 2009, pages 99, XP021062364, DOI: 10.1186/1471-2199-10-99
SEIJ I ISHIIHIROSHI OKUMURACHIYO MATSUBARAFUMI NINOMIYAHIROSHI YOSHIOKA: "Netsukannousei Polima wo Mochiita Suichuyubun no Kani Sokutei Hoho [Simple method of measuring oil-in-water content using heat sensitive polymer", JOURNAL OF WATER AND WASTE, vol. 46, no. 12, 2004
See also references of EP 3819379A4
Attorney, Agent or Firm:
YAMAMOTO Shusaku et al. (JP)
Download PDF:
Previous Patent: AFFIXATION FILM FOR PRINTED WIRING BOARD
Next Patent: NOVEL MICROORGANISMS FOR DECOMPOSING OIL AND FAT CONTAINING FATTY ACID
Next Patent: NOVEL MICROORGANISMS FOR DECOMPOSING OIL AND FAT CONTAINING FATTY ACID