Title:
NOVEL METHOD FOR MEASURING CHOLESTEROL EFFLUX CAPACITY
Document Type and Number:
WIPO Patent Application WO/2022/255351
Kind Code:
A1
Abstract:
The present invention provides a novel method for measuring cholesterol efflux capacity. The present invention provides a method for measuring the cholesterol efflux capacity of HDL, the method including: a step for preparing magnetic beads obtained by converting cholesterol to a solid phase; a step for bringing the magnetic beads obtained by converting cholesterol to a solid phase into contact with an HDL-containing sample to be measured in a solution; and a step for separating the magnetic beads from the solution and measuring the amount of cholesterol in the solution after separating the magnetic beads.
Inventors:
OHKAWA RYUNOSUKE (JP)
MUTSUDA YUME (JP)
HORIUCHI YUNA (JP)
KAMEDA TAKAHIRO (JP)
TOZUKA MINORU (JP)
MUTSUDA YUME (JP)
HORIUCHI YUNA (JP)
KAMEDA TAKAHIRO (JP)
TOZUKA MINORU (JP)
Application Number:
PCT/JP2022/022081
Publication Date:
December 08, 2022
Filing Date:
May 31, 2022
Export Citation:
Assignee:
UNIV NAT CORP TOKYO MEDICAL & DENTAL (JP)
International Classes:
G01N33/68; G01N33/92
Domestic Patent References:
| WO2020185598A1 | 2020-09-17 |
Foreign References:
| JP2020519856A | 2020-07-02 | |||
| JP2007127426A | 2007-05-24 | |||
| JP2019536003A | 2019-12-12 | |||
| JP2020126052A | 2020-08-20 | |||
| US20160274134A1 | 2016-09-22 |
Other References:
HORIUCHI YUNA, LAI SHAO-JUI, YAMAZAKI AZUSA, NAKAMURA AYAKA, OHKAWA RYUNOSUKE, YANO KOUJI, KAMEDA TAKAHIRO, OKUBO SHIGEO, SHIMANO : "Validation and application of a novel cholesterol efflux assay using immobilized liposomes as a substitute for cultured cells", CELL DEATH AND DISEASE, vol. 38, no. 2, 27 April 2018 (2018-04-27), pages 20180144, XP055973160, ISSN: 0144-8463, DOI: 10.1042/BSR20180144
HORIUCHI YUNA, OHKAWA RYUNOSUKE, LAI SHAO-JUI, SHIMANO SHITSUKO, HAGIHARA MICHIO, TOHDA SHUJI, KAMEDA TAKAHIRO, TOZUKA MINORU: "Usefulness of apolipoprotein B-depleted serum in cholesterol efflux capacity assays using immobilized liposome-bound gel beads", CELL DEATH AND DISEASE, vol. 39, no. 4, 30 April 2019 (2019-04-30), pages 20190213, XP093009443, ISSN: 0144-8463, DOI: 10.1042/BSR20190213
堀内優奈 ほか, 固相化リポソーム結合ゲルビーズを用いたコレステロール引き抜き能測定法の従来法との比較, 臨床病理, October 2019, vol. 67 supplement, p. 218
ROHATGI A ET AL.: "HDL cholesterol efflux capacity and incident cardiovascular events", N ENGL J MED., vol. 371, no. 25, 2014, pages 2383 - 93, XP055488506, DOI: 10.1056/NEJMoa1409065
SANKARANARAYANAN ET AL., J LIPID RES, vol. 52, 2011, pages 2332 - 40
A. HARADA ET AL.: "Cholesterol uptake capacity: A new measure of hdl functionality for coronary risk assessment", J. APPL. LAB. MED., vol. 2, no. 2, 2017, pages 186 - 200, XP055665095, DOI: 10.1373/jalm.2016.022913
HORIUCHI Y ET AL.: "Validation and application of a novel cholesterol efflux assay using immobilized liposomes as a substitute for cultures cells", BIOSCI REP., vol. 38, no. 2, 2018, pages 20180144, XP055973160, DOI: 10.1042/BSR20180144
HORIUCHI Y ET AL.: "Usefulness of apolipoprotein B-depleted serum in cholesterol efflux capacity assay using immobilized liposome-bound gel beads", BIOSCI REP., vol. 39, no. 4, 2019, pages 20190213, XP093009443, DOI: 10.1042/BSR20190213
YOSHIMOTO ET AL., BIOTECHNOL. PROG., vol. 22, 2006, pages 459 - 464
HORIUCHI YUNA, OHKAWA RYUNOSUKE, LAI SHAO-JUI, SHIMANO SHITSUKO, HAGIHARA MICHIO, TOHDA SHUJI, KAMEDA TAKAHIRO, TOZUKA MINORU: "Usefulness of apolipoprotein B-depleted serum in cholesterol efflux capacity assays using immobilized liposome-bound gel beads", CELL DEATH AND DISEASE, vol. 39, no. 4, 30 April 2019 (2019-04-30), pages 20190213, XP093009443, ISSN: 0144-8463, DOI: 10.1042/BSR20190213
堀内優奈 ほか, 固相化リポソーム結合ゲルビーズを用いたコレステロール引き抜き能測定法の従来法との比較, 臨床病理, October 2019, vol. 67 supplement, p. 218
ROHATGI A ET AL.: "HDL cholesterol efflux capacity and incident cardiovascular events", N ENGL J MED., vol. 371, no. 25, 2014, pages 2383 - 93, XP055488506, DOI: 10.1056/NEJMoa1409065
SANKARANARAYANAN ET AL., J LIPID RES, vol. 52, 2011, pages 2332 - 40
A. HARADA ET AL.: "Cholesterol uptake capacity: A new measure of hdl functionality for coronary risk assessment", J. APPL. LAB. MED., vol. 2, no. 2, 2017, pages 186 - 200, XP055665095, DOI: 10.1373/jalm.2016.022913
HORIUCHI Y ET AL.: "Validation and application of a novel cholesterol efflux assay using immobilized liposomes as a substitute for cultures cells", BIOSCI REP., vol. 38, no. 2, 2018, pages 20180144, XP055973160, DOI: 10.1042/BSR20180144
HORIUCHI Y ET AL.: "Usefulness of apolipoprotein B-depleted serum in cholesterol efflux capacity assay using immobilized liposome-bound gel beads", BIOSCI REP., vol. 39, no. 4, 2019, pages 20190213, XP093009443, DOI: 10.1042/BSR20190213
YOSHIMOTO ET AL., BIOTECHNOL. PROG., vol. 22, 2006, pages 459 - 464
Attorney, Agent or Firm:
ITO Katsuhiro et al. (JP)
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