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Title:
NOVEL MICROORGANISM, MALTOSE PHOSPHORYLASE, TREHALOSE PHOSPHORYLASE, AND PROCESSES FOR PRODUCING THESE
Document Type and Number:
WIPO Patent Application WO/2005/003343
Kind Code:
A1
Abstract:
A novel microorganism belonging to the genus Paenibacillus which has the ability to produce a maltose phosphorylase and a trehalose phosphorylase; a novel maltose phosphorylase which acts only on maltose to cause the &agr -1,4-glucopyranoside bond in the maltose to reversively undergo phosphorolysis; a novel trehalose phosphorylase which acts only on trehalose to cause the &agr -1,4-glucopyranoside bond in the trehalose to reversively undergo phosphorolysis; and processes for producing these enzymes. The enzymes are more easily obtained than in conventional processes. A considerable reduction in incubation time can also be attained. The enzymes have high stability to temperatures and have almost the same optimum-pH range.

Inventors:
HIDAKA YUKO (JP)
HATADA YUJI (JP)
ITO SUSUMU (JP)
HORIKOSHI KOKI (JP)
YOSHIDA MASAHIRO (JP)
NAKAMURA NOBUYUKI (JP)
TAKADA MASAYASU (JP)
NAKAKUKI TERUO (JP)
Application Number:
PCT/JP2004/009606
Publication Date:
January 13, 2005
Filing Date:
June 30, 2004
Export Citation:
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Assignee:
INDEPENDENT ADMINISTRATIVE INSTITUTION JAPAN AGENCY FOR MARINE EARTH SCI & TECH (JP)
JAPAN MAIZE PROD (JP)
HIDAKA YUKO (JP)
HATADA YUJI (JP)
ITO SUSUMU (JP)
HORIKOSHI KOKI (JP)
YOSHIDA MASAHIRO (JP)
NAKAMURA NOBUYUKI (JP)
TAKADA MASAYASU (JP)
NAKAKUKI TERUO (JP)
International Classes:
C12N1/15; C12N1/19; C12N1/21; C12N9/10; C12N15/31; (IPC1-7): C12N15/09; C12N1/15; C12N1/19; C12N1/20; C12N1/21; C12N9/10; C12N15/31; C12P19/14
Foreign References:
JPH11103885A1999-04-20
JPH10262683A1998-10-06
JPH10327887A1998-12-15
JPH10304881A1998-11-17
JPH0937780A1997-02-10
Other References:
NOLLING, J. ET AL.: "Genome sequence and comparative analysis of the solvent -producing bacterium Clostridium acetobutylicum", J. BACTERIOL, vol. 183, no. 16, 2001, pages 4823 - 4838, XP002984946
HIDAKA, Y. ET AL.: "Shinkai kara Bunri shita Paenibacillus sp.SH-55 Kabu Yurai Trehalose Phosphorylase no Idenshi no Cloning to Kumikae Koso no Shoseishitsu", JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY TAIKAI KOEN YOSHISHU, vol. 2004, 2004, pages 257, XP002984947
Attorney, Agent or Firm:
Mochizuki, Shiro (Kandaizumi-cho Chiyoda-k, Tokyo 24, JP)
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