Title:
NOVEL MODIFIED NUCLEIC ACID SEQUENCES AND METHODS FOR INCREASING mRNA LEVELS AND PROTEIN EXPRESSION IN CELL SYSTEMS
Document Type and Number:
WIPO Patent Application WO1999020766
Kind Code:
A3
Abstract:
The invention provides modified recombinant nucleic acid sequences (preferably DNA) and methods for increasing the mRNA levels and protein expression of proteins which are known to be, or are likely to be, difficult to express in cell culture systems, mammalian cell culture systems, or in transgenic animals. The preferred "difficult" protein candidates for expression using the recombinant techniques of the invention are those proteins derived from heterologous cells preferably those of lower organisms such as parasites, bacteria, and virus, having DNA coding sequences comprising high overall AT content or AT rich regions and/or mRNA instability motifs and/or rare codons relative to the recombinant expression system to be used.
Inventors:
CHEN LI HOW
MEADE HARRY
MEADE HARRY
Application Number:
PCT/US1998/022225
Publication Date:
September 02, 1999
Filing Date:
October 20, 1998
Export Citation:
Assignee:
GENZYME TRANSGENICS CORP (US)
International Classes:
A01K67/027; A61K35/76; A61K39/012; A61K39/12; A61K48/00; C07H21/04; C07K14/445; C07K14/47; C12N1/21; C12N5/10; C12N15/09; C12N15/30; C12N15/67; C12N15/74; C12N15/85; C12P19/34; A61K39/00; (IPC1-7): C12N15/30; C12N15/67; C12N5/10; C12N1/21; C07K14/445; A01K67/027; A61K48/00
Domestic Patent References:
| WO1998014583A2 | 1998-04-09 | |||
| WO1997031115A2 | 1997-08-28 | |||
| WO1991008216A1 | 1991-06-13 |
Foreign References:
| EP0682115A1 | 1995-11-15 |
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