Title:
NOVEL MODIFIED PROTEIN COMPRISING TANDEM-TYPE MULTIMER OF MUTANT EXTRACELLULAR DOMAIN OF PROTEIN G
Document Type and Number:
WIPO Patent Application WO/2013/018880
Kind Code:
A1
Abstract:
The purpose of the present invention is: to provide an excellent protein which is further reduced in the binding property to an Fc region of an immunoglobulin and/or the binding property to an Fab region of the immunoglobulin in a weakly acidic region compared with that of a protein containing an extracellular domain of wild-type protein G, and which still keeps a high antibody-binding activity in a neutral region; and to capture and collect an antibody readily using the protein without denaturating the antibody.
The present invention relates to: a protein that is reduced in the binding property to an Fc region of an immunoglobulin and/or the binding property to an Fab region of the immunoglobulin in a weakly acidic region compared with that of a multimer comprising an extracellular domain of wild type one, which is a domain having a binding activity to a protein comprising an Fc region of immunoglobulin G, while keeping a high antibody-binding activity in a neutral region, and also has a binding activity to a protein comprising the Fc region of immunoglobulin G, wherein the protein comprises a tandem-type multimer of a mutant of the extracellular domain; and others.
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Inventors:
HONDA SHINYA (JP)
MATSUMARU HIROYUKI (JP)
WATANABE HIDEKI (JP)
YOSHIDA CHUYA (JP)
MATSUMARU HIROYUKI (JP)
WATANABE HIDEKI (JP)
YOSHIDA CHUYA (JP)
Application Number:
PCT/JP2012/069794
Publication Date:
February 07, 2013
Filing Date:
August 03, 2012
Export Citation:
Assignee:
NAT INST OF ADVANCED IND SCIEN (JP)
DAICEL CORP (JP)
HONDA SHINYA (JP)
MATSUMARU HIROYUKI (JP)
WATANABE HIDEKI (JP)
YOSHIDA CHUYA (JP)
DAICEL CORP (JP)
HONDA SHINYA (JP)
MATSUMARU HIROYUKI (JP)
WATANABE HIDEKI (JP)
YOSHIDA CHUYA (JP)
International Classes:
C12N15/09; C07K14/315; C07K17/00; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10
Foreign References:
| JP2009297018A | 2009-12-24 | |||
| JPH03501801A | 1991-04-25 | |||
| JP2764021B2 | 1998-06-11 | |||
| JPH03128400A | 1991-05-31 | |||
| JP2003088381A | 2003-03-25 | |||
| JP2009095322A | 2009-05-07 | |||
| JP2009118749A | 2009-06-04 | |||
| JP2009297018A | 2009-12-24 |
Other References:
See also references of EP 2740792A4
BJORCK L; KRONVALL G.: "Purification and some properties of streptococcal protein G, a novel IgG-binding reagent", J IMMUNOL., vol. 133, 1984, pages 969 - 974
BOYLE M. D.P.,: "Bacterial Immunoglobulin Binding Proteins", 1990, ACADEMIC PRESS, INC.
GALLAGHER T; ALEXANDER P; BRYAN P; GILLILAND GL.: "Two crystal structures of the B 1 immunoglobulin-binding domain of streptococcal protein G and comparison with NMR", BIOCHEMISTRY, vol. 19, 1994, pages 4721 - 4729
GAGNON P.: "urification Tools for Monoclonal Antibodies", 1996, VALIDATED BIOSYSTEMS INC.
BJORCK L; KRONVALL G.: "Purification and some properties of streptococcal protein G, a novel IgG-binding reagent", J IMMUNOL., vol. 133, 1984, pages 69 - 74
GALLAGHER T; ALEXANDER P; BRYAN P; GILLILAND GL.: "Two crystal structures of the BI immunoglobulin-binding domain of streptococcal protein G and comparison with NMR", BIOCHEMISTRY, vol. 19, 1994, pages 4721 - 4729
SAUER-ERIKSSON AE; KLEYWEGT GJ; UHLEN M; JONES TA.: "Crystal structure of the C2 fragment of streptococcal protein G in complex with the Fc domain of human IgG", STRUCTURE, vol. 3, 1995, pages 265 - 278, XP004587842, DOI: doi:10.1016/S0969-2126(01)00157-5
DERRICK JP; WIGLEY DB.: "The third IgG-binding domain from streptococcal protein G. An analysis by X-ray crystallography of the structure alone and in a complex with Fab", J MOL BIOL., vol. 243, 1994, pages 906 - 918, XP024008128, DOI: doi:10.1006/jmbi.1994.1691
ALEXANDER P; FAHNESTOCK S; LEE T; ORBAN J; BRYAN P.: "Thermodynamic analysis of the folding of the streptococcal protein G IgG-binding domains BI and B2: why small proteins tend to have high denaturation temperatures", BIOCHEMISTRY, vol. 14, 1992, pages 3597 - 3603
D'SOUZA VM; HOLZ RC.: "The methionyl aminopeptidase from Escherichia coli can function as an iron (II) enzyme", BIOCHEMISTRY, vol. 38, 1999, pages 11079 - 11085
HORTON R. M.; HUNT H. D.; HO S. N.; PULLEN J. M.; PEASE L. R.: "Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension", GENE, vol. 77, 1989, pages 61 - 68, XP023544946, DOI: doi:10.1016/0378-1119(89)90359-4
MASATO OKADA; KAORU MIYAZAKI: "Notes for Protein Experiment", 2004, YODOSHA CO., LTD
SHIGEO OHNO, YOSHIFUMI NISHIMURA: "Protocol for Protein Experiment 1 - Functional Analysis Part", 1997, SHUJUNSHA CO., LTD
SHIGEO OHNO, YOSHIFUMI NISHIMURA: "Protocol for Protein Experiment 2 - Structural Analysis Part", 1997, SHUNJUSHA CO., LTD
VAGIN, A.; TEPLYAKOV, A., JOURNAL OF APPLIED CRYSTALLOGRAPHY, vol. 30, 1997, pages 1022 - 1025
BRUNGER, A. ET AL., ACTA. CRYSTALLOGR. D BIOL. CRYSTALLOGR., vol. 54, 1998, pages 905 - 921
MURSHUDOV, G. N. ET AL., ACTA. CRYSTALLOGR. D BIOL. CRYSTALLOGR., vol. 53, 1997, pages 240 - 255
EMSLEY, P.; COWTAN, K., ACTA. CRYSTALLOGR. D BIOL. CRYSTALLOGR., vol. 60, 2004, pages 2126 - 2132
BJORCK L; KRONVALL G.: "Purification and some properties of streptococcal protein G, a novel IgG-binding reagent", J IMMUNOL., vol. 133, 1984, pages 969 - 974
BOYLE M. D.P.,: "Bacterial Immunoglobulin Binding Proteins", 1990, ACADEMIC PRESS, INC.
GALLAGHER T; ALEXANDER P; BRYAN P; GILLILAND GL.: "Two crystal structures of the B 1 immunoglobulin-binding domain of streptococcal protein G and comparison with NMR", BIOCHEMISTRY, vol. 19, 1994, pages 4721 - 4729
GAGNON P.: "urification Tools for Monoclonal Antibodies", 1996, VALIDATED BIOSYSTEMS INC.
BJORCK L; KRONVALL G.: "Purification and some properties of streptococcal protein G, a novel IgG-binding reagent", J IMMUNOL., vol. 133, 1984, pages 69 - 74
GALLAGHER T; ALEXANDER P; BRYAN P; GILLILAND GL.: "Two crystal structures of the BI immunoglobulin-binding domain of streptococcal protein G and comparison with NMR", BIOCHEMISTRY, vol. 19, 1994, pages 4721 - 4729
SAUER-ERIKSSON AE; KLEYWEGT GJ; UHLEN M; JONES TA.: "Crystal structure of the C2 fragment of streptococcal protein G in complex with the Fc domain of human IgG", STRUCTURE, vol. 3, 1995, pages 265 - 278, XP004587842, DOI: doi:10.1016/S0969-2126(01)00157-5
DERRICK JP; WIGLEY DB.: "The third IgG-binding domain from streptococcal protein G. An analysis by X-ray crystallography of the structure alone and in a complex with Fab", J MOL BIOL., vol. 243, 1994, pages 906 - 918, XP024008128, DOI: doi:10.1006/jmbi.1994.1691
ALEXANDER P; FAHNESTOCK S; LEE T; ORBAN J; BRYAN P.: "Thermodynamic analysis of the folding of the streptococcal protein G IgG-binding domains BI and B2: why small proteins tend to have high denaturation temperatures", BIOCHEMISTRY, vol. 14, 1992, pages 3597 - 3603
D'SOUZA VM; HOLZ RC.: "The methionyl aminopeptidase from Escherichia coli can function as an iron (II) enzyme", BIOCHEMISTRY, vol. 38, 1999, pages 11079 - 11085
HORTON R. M.; HUNT H. D.; HO S. N.; PULLEN J. M.; PEASE L. R.: "Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension", GENE, vol. 77, 1989, pages 61 - 68, XP023544946, DOI: doi:10.1016/0378-1119(89)90359-4
MASATO OKADA; KAORU MIYAZAKI: "Notes for Protein Experiment", 2004, YODOSHA CO., LTD
SHIGEO OHNO, YOSHIFUMI NISHIMURA: "Protocol for Protein Experiment 1 - Functional Analysis Part", 1997, SHUJUNSHA CO., LTD
SHIGEO OHNO, YOSHIFUMI NISHIMURA: "Protocol for Protein Experiment 2 - Structural Analysis Part", 1997, SHUNJUSHA CO., LTD
VAGIN, A.; TEPLYAKOV, A., JOURNAL OF APPLIED CRYSTALLOGRAPHY, vol. 30, 1997, pages 1022 - 1025
BRUNGER, A. ET AL., ACTA. CRYSTALLOGR. D BIOL. CRYSTALLOGR., vol. 54, 1998, pages 905 - 921
MURSHUDOV, G. N. ET AL., ACTA. CRYSTALLOGR. D BIOL. CRYSTALLOGR., vol. 53, 1997, pages 240 - 255
EMSLEY, P.; COWTAN, K., ACTA. CRYSTALLOGR. D BIOL. CRYSTALLOGR., vol. 60, 2004, pages 2126 - 2132
Attorney, Agent or Firm:
ABE MASAHIRO (JP)
Masahiro Abe (JP)
Masahiro Abe (JP)
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