Title:
NOVEL NUCLEIC ACID DERIVATIVE, AND METHOD FOR PREPARING NUCLEASE-RESISTANT NUCLEIC ACID BY USING SAME
Document Type and Number:
WIPO Patent Application WO/2010/021344
Kind Code:
A1
Abstract:
Disclosed is a method for preparing a nuclease-resistant nucleic acid, which comprises the steps of mixing a nucleic acid composed of at least three nucleotides, a terminal deoxynucleotide transferase and a 2’,4’-crosslinked nucleoside triphosphate with one another and incubating the resulting mixture. Examples of the 2’,4’-crosslinked nucleoside triphosphate to be used in the method include 5-methyl-2’-O,4’-C-(methyleneoxy- methylene)uridine-5’-triphosphate, 5-methyl-2’-O,4’-C- (aminomethylene)uridine-5’-triphosphate and 5-methyl-2’-O,4’-C-(benzyloxymethylene)uridine-5’- triphosphate. According to the method, it becomes possible to impart excellent nuclease resistance to an existing functional nucleic acid afterward in a simple manner.
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Inventors:
KUWAHARA MASAYASU (JP)
OBIKA SATOSHI (JP)
OBIKA SATOSHI (JP)
Application Number:
PCT/JP2009/064522
Publication Date:
February 25, 2010
Filing Date:
August 19, 2009
Export Citation:
Assignee:
JAPAN SCIENCE & TECH AGENCY (JP)
KUWAHARA MASAYASU (JP)
OBIKA SATOSHI (JP)
KUWAHARA MASAYASU (JP)
OBIKA SATOSHI (JP)
International Classes:
C12P19/34; C07H19/10; C07H19/20; C12N15/09
Foreign References:
| JP2002521310A | 2002-07-16 |
Other References:
KUWAHARA,M. ET AL.: "Systematic analysis of enzymatic DNA polymerization using oligo-DNA templates and triphosphate analogs involving 2',4'-bridged nucleosides", NUCLEIC ACIDS RES., vol. 36, no. 13, 26 June 2008 (2008-06-26), pages 4257 - 4265
KUWAHARA,M. ET AL.: "Smart conferring of nuclease resistance to DNA by 3'-end protection using 2',4'-bridged nucleoside-5'- triphosphates", BIOORG.MED.CHEM.LETT., vol. 19, no. 11, 1 June 2009 (2009-06-01), pages 2941 - 2943
YOSHIHIKO OGIHARA ET AL.: "2',4'-Kakyogata Nucleoside no Kosoteki DNA Mattan Hyoshiki to Nuclease Taisei no Hyoka", CSJ: THE CHEMICAL SOCIETY OF JAPAN KOEN YOKOSHU, vol. 89TH, no. 2, 13 March 2009 (2009-03-13), pages 1385
KUWAHARA,M. ET AL.: "Smart conferring of nuclease resistance to DNA by 3'-end protection using 2',4'-bridged nucleoside-5'- triphosphates", BIOORG.MED.CHEM.LETT., vol. 19, no. 11, 1 June 2009 (2009-06-01), pages 2941 - 2943
YOSHIHIKO OGIHARA ET AL.: "2',4'-Kakyogata Nucleoside no Kosoteki DNA Mattan Hyoshiki to Nuclease Taisei no Hyoka", CSJ: THE CHEMICAL SOCIETY OF JAPAN KOEN YOKOSHU, vol. 89TH, no. 2, 13 March 2009 (2009-03-13), pages 1385
Attorney, Agent or Firm:
NANJO, Hiromichi (JP)
Hiromichi Nanjo (JP)
Hiromichi Nanjo (JP)
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