Title:
NOVEL NUCLEIC ACID ISOTHERMAL AMPLIFICATION METHOD USING EXTENSION-MEDIATED SELF-FOLDING
Document Type and Number:
WIPO Patent Application WO/2024/101758
Kind Code:
A1
Abstract:
The present invention relates to a novel nucleic acid isothermal amplification method using extension-mediated self-folding. Specifically, the present invention relates to a primer set for extension-mediated self-folding isothermal amplification, a kit for extension-mediated self-folding isothermal amplification comprising the primer set, a target nucleic acid isothermal amplification method using the primer set for extension-mediated self-folding isothermal amplification, and a target nucleic acid detection method using the primer set for extension-mediated self-folding isothermal amplification, the primer set for extension-mediated self-folding isothermal amplification consisting of a pair of primers, which are a forward primer and a reverse primer, wherein the 3' region of each primer comprises an annealing site consisting of 15 to 25 base sequences complementary to a target sequence to be detected, the 5' region of each primer comprises a self-folding site consisting of 10 to 25 base sequences complementary to the nucleic acid sequence of an amplification reaction product so as to form a hairpin structure, and an unnatural base, isodG, is inserted between the annealing site and the self-folding site.
Inventors:
JUNG CHEUL HEE (KR)
SAGONG HA RIM (KR)
SAGONG HA RIM (KR)
Application Number:
PCT/KR2023/017187
Publication Date:
May 16, 2024
Filing Date:
November 01, 2023
Export Citation:
Assignee:
UNIV KOREA RES & BUS FOUND (KR)
International Classes:
C12Q1/6853
Foreign References:
| US20120142059A1 | 2012-06-07 | |||
| KR20220018266A | 2022-02-15 | |||
| KR20170041897A | 2017-04-17 | |||
| KR20210118585A | 2021-10-01 |
Other References:
ÖZAY BURCU, MCCALLA STEPHANIE E: "A review of reaction enhancement strategies for isothermal nucleic acid amplification reactions", SENSORS AND ACTUATORS REPORTS, vol. 3, 1 November 2021 (2021-11-01), pages 100033, XP093070439, ISSN: 2666-0539, DOI: 10.1016/j.snr.2021.100033
SAGONG HARIM, JUNG CHEULHEE: "Development of extension-mediated self-folding isothermal amplification technology for SARS-CoV-2 diagnosis", BIOSENSORS AND BIOELECTRONICS, vol. 237, 1 October 2023 (2023-10-01), pages 115516, XP093170416, DOI: 10.1016/j.bios.2023.115516
SAGONG HARIM, JUNG CHEULHEE: "Development of extension-mediated self-folding isothermal amplification technology for SARS-CoV-2 diagnosis", BIOSENSORS AND BIOELECTRONICS, vol. 237, 1 October 2023 (2023-10-01), pages 115516, XP093170416, DOI: 10.1016/j.bios.2023.115516
Attorney, Agent or Firm:
NAM IP GROUP (KR)
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