NOVEL OPTICAL ARCHITECTURES FOR MICROVOLUME LASER-SCANNING CYTOMETERS

Title:

NOVEL OPTICAL ARCHITECTURES FOR MICROVOLUME LASER-SCANNING CYTOMETERS

Document Type and Number:

WIPO Patent Application WO2000011024

Kind Code:

A3

Abstract:

Instrumentation and methods for performing charged coupled device (CCD) based confocal spectroscopy with a laser spot array are provided. A sample is scanned by a plurality of excitation laser scanning spots. The scanning spots are generated, for example, by converting a laser excitation beam into an array of beams using a multiple beam generating optic, such as a Damann grating. Each scanning spot scans and excites a separate region of the sample. Fluorescent light emitted by the sample is collected and imaged onto the surface of a CCD detector that has been electronically divided into bins of pixels, with each bin corresponding to one of the scanning spots. Each bin functions as a confocal aperture in which the confocal depth of focus can be controlled by changing the dimensions of the bin. Plural CCD detectors can be used for multichannel imaging of individual wavelengths. The instrumentation and methods are useful in any spectroscopic application including microscopy and microvolume laser scanning cytometry (MLSC).

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Inventors:

DIETZ LOUIS J
WALTON IAN
NORTON SCOTT

Application Number:

PCT/US1999/019374

Publication Date:

June 29, 2000

Filing Date:

August 20, 1999

Export Citation:

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Assignee:

SURROMED (US)

International Classes:

G01N21/64; C12M1/34; C12Q1/06; G01N15/14; G01N33/483; (IPC1-7): G01N21/64

Foreign References:

US5726751A	1998-03-10
US5446532A	1995-08-29

Other References: