BACKGROUND OF THE INVENTION Field of Invention [0001] The invention telates to new 2,3-benzodiazepine derivatives substituted by heterocycles, the acid addition salts thereof, as well as the pharmaceutical compositions containing them. The invention also relates to the use of said compounds as AMPA receptor antagonists.

Summary of Related Art [0002] Over-activation of glutamate receptors has been associated with several acute and chronic diseases of the central nervous system ("CNS"). Various glutamate receptor antagonists have been investigated as therapeutic modalities (see for example Parsons et al., Dniu Neus Perspect. 11: 523 (1998) and Brauner-Osbome et al., J. Med. Chem. 43: 2609 (2000)).

[0003] AMPA (2-amino-3- (3-hydroxy-5-methyl-4-isoxazolyl)-propionic acid) type glutamate receptors play a major role in a variety of central nervous system disorders. Inhibition of the activation of AMPA type receptors has been shown to have neuroprotective, antiepileptic and muscle-relaxant effects (see e.g., Cerebrovasc. Brain Metab. Rev. 6 : 225 (1994); Neaarology 44 Suppl. 8, S14 (1994); J. Phamiacoz E, % t. Ther. 260: 742 (1992)).

[0004] Glutamate receptors have been found not only in the CNS but also in peripheral tissues indicating therapeutic potential opportunities beyond the CNS (see e., Skery et al., Trends in Phora. SGi., 92 : 74 (2001). Respiratory tract inflarnmation has been postulated to be beneficially influenced by NMDA-type glutamate antagonists (Said, Trends in Pharm. Sci. 20: 132 (1999); and Said et al., Trends in Pharm. Sci., 22: 344 (2001)).

[0005] AMPA type receptors can be inhibited by various competitive and non- competitive antagonists. The therapeutic potential of non-competitive antagonists may be superior to that of competitive ones insofar as their activity is not dependent on high concentrations of endogenous glutamate (see eg, Vizi et aL, CNS DrugRev, 2 : 91 (1996)). One of the most prominent non-competitive AMPA receptor antagonists is 5- (4-aminophenyl)-8- methyl-9H-1, 3-dioxolo [4, 5-h] [2,3] benzodiazepine (also designated as GYKI 52466) possessing remarkable antiepileptic, muscle relaxant and neuroprotective activities. (Tarnawa eal,. Eur. J.

Phamzl., 167: 193 (1989) ;. Smith, et al., Eur. J. Pharmacol., 187: 131 (1990); Quardouz etat, Nemså Ldtt., 125: 5 (1991); Donevan et al., I. Nez, 10 : 51 (1993)).

[0006] Several non-competitive AMPA antagonists have been described in the literature including 3, 4-dihydro-5H-or 4, 5-dihydro-3H-2,3-benzodiazepines, containing an acyl group at position 3 of the ring (see eg., Hungarian Patent Nos. 206,719 B and 219,777 B, U. S. Patent No.

5,536, 832, European Patent Publication No. 0699 677 Al, and British Patent No. 2 311 779, as well as WO 96/04 283, WO 97/28 135, WO 99/07 707, WO 99/07 708 and WO 01/04 122).

WO 96/06 606 (corresponding to U. S. Patent No. 5,795, 886) describes several 2, 3- benzodiazepine derivatives having aryl and heteroaryl substituents (e.g., pyridyl, thienyl, furyl, phenyl, imidazolyl benzimidazolyl, et.) at C3.

[0007] The compounds listed above have been found to be particularly useful in diseases, in which the over-function of the glutamate system can be detected. Such acute disorders of the CNS include for example stroke, brain ischemia, brain and spinal cord injuries, perinatal hypoxia, hypoglycemic nervous damage, etc. Additional chronic illnesses in which selected AMPA antagonists can be applied include eg., Alzheimer's disease, Huntington's disease, amyotrophic lateral sclerosis, AIDS-induced dementia, glaucoma, diabetic retinopathy as well as Parkinson's disease. Furthermore, enhanced activity of the glutamate system has also been shown in conditions associated with neural damage (eg, epilepsy, migraine, urinary bladder incontinence, psychosis -- anxiety, schizophrenia etc., drug-abuse, pathological pain, brain edema and tardive dyskinesia) implying an impressive therapeutic potential for AMPA antagonists.

[0008] Recently, experimental data suggested that selected AMPA antagonists have beneficial effect on the autoimmune encephalomyelitis elicited in rats, which is the accepted model of multiple sclerosis (Smith et Nature Medicine 6: 62 (2000) ). In addition, AMPA and NMDA receptors in the spinal cord have been implicated in the contraction of the bladder and the urethra, suggesting that AMPA antagonists maybe useful in the treatment of urinary incontinence (Nishizawa et al., A du in Exp. Med. & Biol. 4: 275 (1999)).

[0009] Two 2,3-benzodiazepine derivatives GYKI 52466 (supra), and (R)-7-acetyl-5- (4- aminophenyl)-8, 9-dihydro-8-methyl-7H-1, 3-dioxolo [4, 5-h] [2, 3] benzodiazepine (GYKI 53773, also known as Talampanel) were beneficial. The latter has proved to be active in clinical trials on epilepsy patients (Bialer et al., Epilepsy Res. 43: 11 (2001)).

[0010] In addition, GYKI 52466 has been reported to inhibit growth of selected tumor cell types (colon adenocarcinoma, astrocytoma, breast carcinoma, lung carcinoma and neuroblastoma) (Rzeski et al., Proc. Nat. Acad. Sci. 98: 6372 (2001)).

SUMMARY OF THE INVENTION [0011] The invention relates to new 2,3-benzodiazepine derivatives of formula (I) below, isomers and acid addition salts thereof and to pharmaceutical compositions containing the same, 0 wherein the substituent meanings are as follows : R3 represents a substituted or unsubstituted 5-or 6-membered, aromatic, saturated or partially saturated heterocyclic ring containing at least 2 heteroatoms, in which the heteroatom can be oxygen-, sulfur-or nitrogen atom and in the case when the heterocyclic ring contains 2 heteroatoms one of them is different from nitrogen; R4, R5, R6 and R7 independentlyfrom each other represent hydrogen atom, halogen atom, C1-C3 alkyl group, nitro group or amino group, wherein the amino group can be substituted independently from each other with one or two C1-C3 alkyl group, C2-Cs acyl group, or C2-Cs alkoxycarbonyl group, or aminocarbonyl group, or Cz-C5 allylaminocarboni group; and R9 represents C1-C3 alkoxy group or halogen atom R10 represents hydrogen or halogen atom or R9 and R10 together can be Cl-C3 alkylendioxy group.

[0012] Representative compounds include, without limitation, (R)-5- (4-aminophenyl)-8- methyl-7-(5-methyl-1, 3, 4-thiadiazol-2-yl)-8, 9-dihydro-7H-1, 3-dioxolo [4,5-h] [2,3] benzodiazepine; (R) -5- (4-aminophenyl)-8-methyl-7- (1, 3, 4-thiadiazol-2-yl)-8, 9-dihydro-7H-1, 3-dioxolo [4,5- h] [2,3] benzodiazepine ; (R)-5- (4-aminophenyl)-8-methyl-7- (2-thiazolyl)-8, 9-dihydro-7H-1, 3- dioxolo [4,5-h] [2,3] benzodiazepine ; (R)-5- (4-aminophenyl)-7- (4, 5-dihydro-thiazol-2-yl)-8-methyl- 8, 9-dihydro-7H-1, 3-dioxolo [4,5-h] [2,3] benzodiazepine ; (R)-5-(4-aminophenyl)-7-(5-ethyl-1, 3,4- thiadiazol-2-yl)-8-methyl-8, 9-dihydro-7H-1, 3-dioxolo [4,5-h] [2,3] benzodiazepine ; (R) -5- (4- aminophenyl)-8-methyl-7- (5-methyl-1, 3, 4-oxadiazol-2-yl)-8, 9-dihydro-7H-1, 3-dioxolo [4,5- <BR> <BR> <BR> h] [2,3] benzodiazepine; (R) -5- (4-amino-3-methylphenyl)-8-methyl-7- (5-methyl-1, 3, 4-thiadiazol-2- yl)-8, 9-dihydro-7H-1, 3-dioxolo- [4, 5-h] [2,3] benzodiazepine ; (R)-5- (4-amino-3-methylphenyl)-7- (5- ethyl-1, 3, 4-thiadiazol-2-yl)-8-methyl-8, 9-dihydro-7H-1, 3-dioxolo- [4, 5-h] [2,3] benzodiazepine ; (R)- 5- (4-amino-3-methylphenyl)-8-methyl-7-(5-propyl-1, 3, 4-thiadiazol-2-yl)-8,9-dihydro-7H-1, 3- dioxolo [4,5-h] [2,3] benzo-diazepine; (R)-5- (4-amino-3-methylphenyl)-8-methyl-7- (1, 3,4- thiadiazol-2-yl)-8, 9-dihydro-7H-1, 3-dioxolo- [4, 5-h] [2,3] benzodiazepine ; (R)-5- (4-amino-3- methylphenyi)-8-methyl-7- (5-methoxymethyl-1, 3, 4-thiadiazol-2-yl)-8,9-dihydro-7H-1, 3-dioxolo- [4,5-h] [2,3] benzodiazepine ; (R)-5- (4-amino-3-methylphenyl)-8-methyl-7- {5- [1- (lE)-propen-1-yl]- 1, 3, 4-thiadiazol-2-yl}-8, 9-dihydro-7H-1, 3-dioxolo [4,5-h] [2,3] benzodiazepine ; (R)-5- (4-amino-3- chlorophenyl)-8-methyl-7- (5-methyl-1, 3, 4-thiadiazol-2-yl)-8, 9-dihydro-7H-1, 3-dioxolo- [4, 5- h] [2,3] benzodiazepine ; and (R)-5- (4-amino-3-chlorophenyl)-8-methyl-7- (5-methoxymethyl-1, 3,4- thiadiazol-2-yl)-8, 9-diliydro-7H-1, 3-dioxolo [4,5-h] [2,3] benzodiazepine and the acid addition salts thereof.

[0013] The invention also discloses pharmaceutical compositions comprising a compound of formula (I) as the active ingredient, wherein the meaning of R3-R7, RI and Rí° is as defined herein, or a steroisomer or a pharmaceutically acceptable salt thereof together with pharmaceutically acceptable solvents, diluents, carriers and filling materials.

[0014] The compounds are suitable for treating conditions associated with muscle spasms, epilepsy, acute and chronic forms of neurodegenerative diseases as well as preventing, treating or alleviating the symptoms of acute and chronic inflammatory disorders.

[0015] One of skill will appreciate, in light of the many publications relating to the expanding therapeutic values of AMPA type receptor antagonists, that the compounds of the invention are useful in a very large number of unrelated conditions.

[0016] Hence, methods for treating glutamate dysfunction associated with an acute or chronic neurodegenerative disease or in acute or chronic disease of the eyes associated with glutamate dysfunction are provided. Representative neurodegenerative disorders include, for example, cerebral ischemia (stroke), brain and spinal cord trauma, Alzheimer's disease, Huntington's disease, amyotrophic lateral sclerosis, AIDS-induced dementia, essential tremor, Parkinson's disease, multiple sclerosis and urinary incontinence. Acute or chronic disorders of the eyes associated-with glutamate dysfunction include glaucoma or diabetic retinopathy.

Disclosed also are methods for treating epilepsy, reducing muscle spasms, reducing pain, or inflammatory disorders which comprise administering to the subject in need of such treatment a therapeutically effective amount of the compounds of the invention. Included among the inflammatory disorders are allergic inflammatory disorders of the airways which can encompass allergic rhinitis, intrinsic or extrinsic asthma bronchiale, acute or chronic bronchitis, chronic obstructive pulmonary disease and pulmonary fibrosis.

DETAILED DESCRIPTION OF THE INVENTION [0017] The patents, published applications, and scientific literature referred to herein establish the knowledge of those skilled in the art and are hereby incorporated by reference in their entirety to the same extent as if each was specifically and individually indicated to be incorporated by reference. Any conflict between any reference cited herein and the specific teachings of this specifications shall be resolved in favor of the latter. Likewise, any conflict between an art-understood definition of a word or phrase and a definition of the word or phrase as specifically taught in this specification shall be resolved in favor of the latter.

[0018] The invention discloses novel substituted 2,3-benzodiazepine derivative compounds and methods of making the same. Pharmaceutical compositions employing the novel substituted 2, 3-benzodiazepine derivative compounds and their use for the treatment for a number of disease conditions are also disclosed.

[0019] Technical and scientific terms used herein have the meaning commonly understood by one of skill in the art to which the present invention pertains, unless otherwise defined. Reference is made herein to various methodologies and materials known to those of skill in the art. Standard reference works setting forth the general principles of pharmacology include Goodman and Gilman's The Pharmacological Basis of Therapeutics, 10th Ed. , McGraw Hill Companies Inc., New York (2001). Any suitable materials and/or methods known to those of skill can be utilized in carrying out the present invention. However, preferred materials and methods are described. Materials, reagents and the like to which reference are made in the following description and examples are obtainable from commercial sources, unless otherwise noted.

[0020] As used in this specification, the singular forms"a","an"and"the"specifically also encompass the plural forms of the terms to which they refer, unless the content clearly dictates otherwise. For example, reference to"an antagonist"includes mixtures of antagonists.

[0021] As used in this specification, whether in a transitional phrase or in the body of the claim, the terms"comprise (s)" and"comprising"are to be interpreted as having an open- ended meaning. That is, the terms are to be interpreted synonymously with the phrases"having at least"or"including at least". When used in the context of a process, the term"comprising" means that the process includes at least the recited steps, but may include additional steps.

When used in the context of a compound or composition, the term"comprising"means that the compound or composition includes at least the recited features or components, but may also include additional features or components.

[0022] The term"about"is used herein to mean approximately, in the region of, roughly, or around. When the term"about"is used in conjunction with a numerical range, it modifies that range by extending the boundaries above and below the numerical values set forth.

In general, the term"about"is used herein to modify a numerical value above and below the stated value by a variance of 20%.

[0023] As used herein, unless specifically indicated otherwise, the word"or"is used in the"inclusive"sense of"and/or"and not the"exclusive"sense of"either/or".

[0024] As used herein, the recitation of a numerical range for a variable is intended to convey that the invention may be practiced with the variable equal to any of the values within that range. Thus, for a variable which is inherently discrete, the variable can be equal to any integer value of the numerical range, including the end-points of the range. Similarly, for a variable which is inherently continuous, the variable can be equal to any real value of the numerical range, including the end-points of the range. As an example, a variable which is described as having values between 0 and 2, can be 0,1 or 2 for variables which are inherently discrete, and can be 0.0, 0.1, 0.01, 0.001, or any other real value for variables which are inherently continuous.

[0025] The methods of the present invention are intended for use with any mammal that may experience the benefits of the methods of the invention. Foremost among such mammals are humans, although the invention is not intended to be so limited, and is applicable to veterinary uses. Thus, in accordance with the invention,"mammals"or"mammal in need" include humans as well as non-human mammals, particularly domesticated animals including, without limitation, cats, dogs, and horses.

[0026] It will be understood that the subject to which a compound of the invention is administered need not suffer from a specific traumatic state. Indeed, the compounds of the invention may be administered prophylactically, prior to any development of symptoms. The term"therapeutic","therapeutically", and permutations of these terms are used to encompass therapeutic, palliative as well as prophylactic uses. Hence, as used herein, by"treating or alleviating the symptoms"is meant reducing, preventing, and/or reversing the symptoms of the individual to which a compound of the invention has been administered, as compared to the symptoms of an individual receiving no such administration.

[0027] Benzodiazepines of formula (II) below, and the isomers as well as the acid addition salts thereof, are the subject of Patent Application No. 10/358,053 : all wherein RI and R2 independentlyof each other represent hydrogen atom or Cl-C3 alkyl group, R3 represents substituted or unsubstituted 5-or 6-membered, aromatic, saturated or partially saturated heterocyclic ring containing at least 2 hetero atoms, in which the hetero atom can be oxygen-, sulfur-or nitrogen atom and in the case when R3 is a 5-membered ring one of the heteroatoms is different from nitrogen; R4, R5, R6, R7 and R8 independently from each other represent hydrogen atom, halogen atom, Cl-C3 alkyl group, nitro group or amino group, wherein the amino group can be substituted independently from each other with one or two Ci-C3 alkyl group, C2-Cs acyl group, or C2-Cs alkoxycarbonyl group, or aminocarbonyl group, or C2-C5 alkylaminocarbonyl group, R9 represents Ci-C3 alkoxy group or halogen atom, RIO represents hydrogen or halogen atom or R9 and R10 together can be Ci-C3 alkylendioxy group.

[0028] The present invention is directed to 2,3-benzodiazepine derivatives of formula (H) as shown below in formula (I) : (l) wherein Rl and R8 are hydrogen, R is CH3, the meaning of R4, R5, R6, R7, R9 and RIO is as defined above, R3 is a moiety selected from the group consisting of substituted or unsubstituted isoxazole, isothiazole, thiazole, thiazoline, 4-thiazolinone, oxazole, oxazoline, 1, 2, 3-thiadiazole, 1,3, 4-thiadiazole, 1, 3, 4-thiadiazolin-2-one, 1, 2, 4-thi, diazolin-3-one, 1, 4, 2-oxathiazoline, 1,3, 4- oxadiazole, 1,2, 3-triazole, 1,3,4-triazole, 1, 2,3, 4-thiatriazole,. tetrazole, 1, 3-thiazin-4-one and 1, 3, 4-thiadiazin-4-one ring.

[0029] The heterocyclic substituent of the benzodiazepine ring as R3 can be further substituted-among others-with one or more Cl-C6 alkyl group, C2-C3 allcenyl, a C3-C7 cycloalkyl, a trifluoromethyl, a C1-C3 alkoxy or a phenyl group, an oxo, a formyl, a carboxyl or a C2-C4 alkoxyzarbonyl group, a Cl-C3 alkoxymethyl group, a hydroxymethyl group, whrerein the hydroxy group can be alkylated or acylate, a Cl-C3 alkylthiomethyl group, a cyanomethyl group or an aminomehtyl group, wherein the amino group can be alkylated or acylated.

[0030] The meaning of alkyl group encompasses both straight and branched chain allyl groups. The meaning of alkenyl group can be vinyl, 1-propenyl or 2-propenyl group. The meaning of halogen atom can be fluorine, chlorine, bromine, or iodine atom. The amino group can be unsubstituted or substituted with one or two alkyl groups, as well as acylated with aliphatic or aromatic carboxylic acid or any kind of carbonic acid esters.

[0031] In the case of compounds of formula (I), the term"isomers"means both enantiomers, as well as the E and Z isomers if applicable, furthermore, isomers shall include diastereomers, tautomers and mixture of them, for example racemic mixture.

[0032] Salts of the compounds of formula (I) relate to physiologicallyacceptable salts formed with inorganic or organic acids. Suitable inorganic acids can be, for example, hydrochloric acid, hydrobromic acid, phosphoric acid or sulfuric acid. Suitable organic acids can be, for example, formic acid, acetic acid, maleic and fumaric acid, succinic acid, lactic acid, tartaric acid, citric acid or methanesulfonic acid.

[0033] Representative compounds of formula (I) include, without limitation, (R)-5- (4- aminophenyl)-8-methyl-7- (5-methyl-1, 3, 4-thiadiazol-2-yl)-8, 9-dihydro-7H-1, 3-dioxolo [4,5- h] [2,3] benzodiazepine ; (R)-5- (4-aminophenyl)-8-methyl-7- (1, 3, 4-thiadiazol-2-yl)-8, 9-dihydro-7H- 1, 3-dioxolo [4, 5-h] [2, 3] benzodiazepine ; (R)-5- (4-aminophenyl)-8-methyl-7- (2-thiazolyl)-8, 9- dihydro-7H-1, 3-dioxolo [4, 5-h] [2, 3] benzodiazepine ; (R)-5- (4-aminophenyl)-7- (4,5-dihydro- thiazol-2-yl)-8-methyl-8, 9-dihydro-7H-1, 3-dioxolo [4,5-h] [2, 3] benzodiazepine ; (R)-5- (4- aminophenyl)-7- (5-ethyl-1, 3, 4-thiadiazol-2-yl)-8-methyl-8,9-dihydro-7H-1,3-dioxolo[4,5- h] [2, 3] benzodiazepine ; (R)-5- (4-aminophenyl)-8-methyl-7- (5-methyl-173, 4-oxadiazol-2-yi)-8, 9- <BR> <BR> <BR> dihydro-7H-ßdioxolo [495-h] [2 3] benzodiazepineS (R) -5- (4-amino-3-methylphenyl)-8-methyl-7- (5-methyl-1, 3, 4-thiadiazol-2-yl)-8, 9-dihydro-7H-l, 3-dioxolo- [4, 5-h] [2, 3] benzo-diazepine; (R) -5- (4-amino-3-methylphenyl)-7- (5-ethyl-1,3,4-thiadiazol-2-yl)-8-methyl-8,9-dihydro-7H-1,3- dioxolo- [4,5-h] [2,3] benzodiazepine; (R) -5- (4-amino-3-methylphenyl)-8-methyl-7- (5-propyl-1, 3,4- thiadiazol-2-yl)-8, 9-dihydro-7H-1, 3-dioxolo [4,5-h] [2, 3]benzo-diazepine ; (R)-5- (4-amino-3- methylphenyl)-8-methyl-7- (1, 3, 4-thiadiazol-2-yl)-8, 9-dihydro-7H-1, 3-dioxolo- [4, 5- h] [2,3] benzodiazepine ; (R)-5- (4-amino-3-methylphenyl)-8-methyl-7- (5-methoxymethyl-1, 3,4- thiadiazol-2-yl)-8,9-dihydro-7H-1,3-dioxolo-[4,5-h][2,3]benz odiazepine; (R)-5-(4-amino-3- methylphenyl)-8-methyl-7- {5- [l- (lE)-propen-1-yl]-1, 3, 4-thiadiazol-2-yl}-8, 9-dihydro-7H-1, 3- dioxolo [4, 5-h] [2,3] benzodiazepine ; (R)-5- (4-amino-3-chlorophenyl)-8-methyl-7- (5-methyl-1, 3,4- thiadiazol-2-yl)-8, 9-dihydro-7H-1, 3-dioxolo- [4, 5-h] [2,3] benzodiazepine ; and (R)-5- (4-amino-3- chlorophenyl)-8-methyl-7- (5-methoxymethyl-1,3,4-thiadiazol-2-yl)-8,9-dihydro-7H-1,3- dioxolo [4, 5-h] [2,3] benzodiazepine and the acid addition salts thereof.

[0034] The compounds of formulas (I) and (II) can be prepared in the following way.

The heterocycle corresponding to R3 is built up starting from a compound of formula (III) below : (111) wherein Rl-R10 are defined for formulas (I) and (II) above, by known methods or a compound having the following formula (IV) or the following isochromeniliurn salt having formula (¢Va) which is formed from the compound of formula (IV), wherein the meaning of R1, R2, R4, R5, R6, R7, R8, R9 and R10 is as defined above: (fiv) (IVa) A compound of formulas (IV) or (IVa) is reacted with a compound having formula (V) or (VI) : wherein the meaning of R3 is as defined above and the meaning of Rll is C2-Ce alkoxycarbonyi or aryl alkoxycarbonyl group to obtain the compounds of formulas (VII) or (VIII).

(Vil) (VIII) [0035] The hydroxyl group of the compounds of formulas (VII) or (VIII) is transformed into a sulfonate ester, and the latter intermediate is submitted to ring-closure resulting in compounds of formulas (I), (II) or (IX) : (IX) by applying a strong base. Alternatively, the compounds of formulas (VII) or (VIII) are transformed into compounds of formulas (I), (II) or (IX) according to Mitsunobu (Syrhis, I : 1 (1988) ). In the compound of formula (fizz the Rll group is cleaved to give the compound of formula (III), which is converted into the compound of formulas (I) or (II) according to the method described in process as described above. Then, if desired, in a compound of formula or (II) obtained according to any of the above processes, the nitro group is reduced or the amino group is acylated, alkylated, or after diazotation, is exchanged by a halogen atom or hydrogen atom, or a halogen atom is exchanged by an amino group and in this'way it is transformed into another compound of formula (I) or (II) and/or the isomers are separated and, if desired, salts are formed.

[0036] The compounds of formulas (III) and (IX) are chiral compounds, and therefore formulas (III) and (IX) refers to either of the individual enantiomers or mixtures thereof. The hemiketal type compounds of formula (IV) as well as the hydrazone derivatives of formulas (VII) and (VIII) represent different stereoisomers and they refer to all of the individual stereoisomers and mixtures thereof. The Rll group can be a C2-C8 alkoxycarbonyl group, such as a tert-butoxycarbonyl or a benzyloxycarbonyl group.

[0037] The starting materials of formula (III) are known in the literature (U. S. Patent No. 5,536, 832 and British Patent No. 2,311, 779, as well as WO 97/28 135 and WO 01/04 122).

Hungarian Patent No. 219,777 and British Patent No. 2,311, 779 describe the synthesis of optically active compounds of formula (III) as well.

[0038] The optically active compounds of formula (III) can be synthesized by reacting a hemiketal of formula (IV) - prepared for example from an optically active substituted phenyl- isopropanolaccordingtoAndersonetat (T. Am (} em Soc. 117: 12358 (1995))-with an alkoxycarbonyl-hydrazide containing an easily removable alkoxycarbonyl group, such as a tert- butoxy-carbamate in the presence of catalytic amount of an acid. The hydrazone of formula (VIII) obtained after isolation then is transformed into a mesyl ester eg, with methanesulfonyl chloride in the presence of triethylamine, and the latter is treated with base, for example sodium hydroxide, in alcoholic solution to yield the benzodiazepine derivative of formula (IX) in a ring closure reaction. Then the substituent of the N3 atom (numbering according to the benzodiazepine ring) is cleaved, eg, by hydrolysis or another method, for example hydrogenolysis, to yield the desired compound of formula (III). The cleavage of the tert- butoxycarbonyl group may be carried out with trifluoroacetic acid, hydrogen bromide or zinc bromide in dichloromethane.

[0039] The heterocyclic moiety-corresponding to the R3 substituent-of the compound of formula (I) or (II) is synthesized starting from the compounds of formula (III) according to methods known in the art relating to heterocyclic chemistry.

[0040] Some of the compounds of formula (I) or (II) can be synthesized, for example, from the 4, 5-dihydro-2, 3-benzodiazepine derivatives substituted with thiocarbamoyl group at position 3 of the benzodiazepine ring. Latter compounds can be obtained from 4, 5-dihydro-3H- 2,3-benzodiazepine derivatives of formula (III), for example with potassium thiocyanate in acetic acid medium. The thus-obtained 4, 5-dihydro-3-thiocarbamoyl-3H-2, 3-benzodiazepines are reacted with a-halo ketones or oc-halo aldehyde acetals to yield 2,3-benzodiazepine derivatives having a substituted or unsubstituted 2-thiazolyl group. In an analogous reaction, if 2-halo carboxylic acid esters are used instead of the a-halo oxo-compound, the appropriate compounds containing a 3-thiazolinone ring are formed.

[0041] When the above-mentioned 4,5-dihydro-2, 3-benzodiazepines containing thiocarbamoyl group in position 3 are reacted with D-halo carboxylic acid esters, for example ethyl 3-bromopropionate, then new 2, 3-benzodiazepine derivatives substituted with 5,6-dihydro- [1, 3] thiazin-4-one ring are obtained.

[0042] The compounds of formula (I) or (II) containing 1,3, 4-thiadiazole group as R3 substituent can be synthesized for example by the following way. First, a trimethylsilyl derivative is prepared from a 4, 5-dihydro-3H- [2, 3] benzodiazepine of formula (III), which is then reacted with thiophosgene to give thiocarboxylic acid chloride. Finally, the latter is treated with hydrazine to yield the thiocarboxytic acid hydrazide derivatives. The 2,3-benzodiazepine derivatives substituted with carbothiohydrazide group are reacted with an acid anhydride or chloride and the thus-obtained partially occurring ring closure of the carbotlio-N-acylhydrazides is promoted byfurther acid treatment to yield [1, 3, 4] thiadiazolyl-2, 3-benzodiazepines. lnother procedure for the synthesis of the latter compounds is to react the above-mentioned intermediate thiocarboxylic acid chloride with an acid hydrazide, and then the resulting carbothiohydrazide derivative containing an acyl group on the terminal N-atom is treated with acid to give the cyclic product.

[0043] In an analogous reaction benzodiazepines of formula (I) or (II) containing a [1, 3,4] oxadiazole ring can be obtained, for example, if the above mentioned N-acyl- thiocarboxylic acid hydrazide derivative is treated with a sulfur binding reagent, for example mercury (II) acetate.

[0044] The 4,5-dihydro-2, 3-benzodiazepin-3-carbothiohydrazides can serve as starting materials for further new compounds of formula (I) or (II) substituted with a hetero-ring. For example, if the N-methyl-carbamoyl-carbothiohydrazide obtained with methyl isocyanate is heated with concentrated acid, for example hydrochloric acid, then new compounds of formula (I) or (II) substituted with (5-oxo-4, 5-dihydro- [1, 3,4] thiadiazol-2-yl) group can be obtained. If the carbothiohydrazide derivative is reacted with bromoacetic acid ester, (5-oxo-5, 6-dihydro-4H- [1, 3,4] thiadiazin-2-yl)-[2, 3] benzodiazepine derivatives having a 6-membered ring as the R3 substituent are obtained. If the carbothiohydrazide derivatives are reacted with a a-halo- ketones, for example chloroacetone, then eg, (5-methyl-6H-[1, 3,4] thiadiazin-2-yl)- [2,3] benzodiazepines are formed.

[0045] The appropriate thiohydroxamic acids can be obtained from [2,3] benzodiazepin- 3-thiocarboxylic acid chlorides with hydroxylamine, which can be transformed into heterocyclic compounds by reacting with bifunctional allylating agents. Among others, [1, 4,2] oxathiazol-3- yi-2, 3-benzodiazepines can be synthesized for example from thiohydroxamic acid derivatives with methylene iodide.

[0046] The compounds of formula (I) or (II) containing 3-oxo-2,3-dihydro- [1, 2,4] thiadiazol-5-yl group as R3 substituent can be prepared, for example, by reacting the unsubstituted compounds of formula (III) with phenoxycarbonyl isothiocyanate, then the resulting phenoxycarbonyl-thiocarbamoyl-benzodiazepine transformed into N-allyl-carbamoyl- thiocarbamoyl-benzodiazepine with primary amines and the latter is reacted ag, with bromine to accomplish the ring closure between the sulfur and the nitrogen atoms.

[0047] The compounds of formula (I) or (II) containing 4, 5-dihydro-oxazol-2-yi group as an R3 substituent can be synthesized by reacting the compounds of formula (III) with chloroethyl isocyanate to give an urea derivative, which is heated in the presence of sodium iodide and potassium carbonate in dimethylformamide to accomplish the ring closure.

[0048] The compounds of formula (X) or (II) containing 2-alliyl-thiazol-4-yl group as R3 substituent can be synthesized by reacting 3-bromo-acetyl- [2, 3] benzodiazepines with the appropriate carboxylic acid thioamide.

[0049] From 3-cyano-2, 3-benzodiazepines-obtained from 2, 3-benzodiazepines of formula (III) with cyanogen bromide-2, 3-benzodiazepines containing among others (1H- tetrazol-5-yl) as well as (5-alkyl- [l, 2,4] oxadiazol-3-yl) groups as an R3 substituent can be synthesized. The tetrazolyl compounds can be synthesized by reacting the nitrile derivative-with sodium azide in dimethylformamide in the presence of ammonium chloride, while if the nitrile compound is first treated with hydroxylamine and the thus-obtained amidoxime is reacted with a carboxylic acid anhydride or chloride, then the appropriate 1, 2, 4-oxadiazolyl compounds can be obtained.

[0050] The compounds of formula (I) or (II) containing 1,2, 4-triazolyl group as R3 substituent can be synthesized from a 3-thiocarbamoyl- [2, 3] benzodiazepine derivative by reacting first with methyl iodide, then the obtained S-methyl compound is condensed with hydrazine and the so formed intermediate is treated with a carboxylic acid anhydride or chloride.

[0051] Other illustrative processes for the synthesis of compounds of formula (I) or (II) are those, where a hemiketal of formula (IV) is reacted with a heterocyclic reagent substituted with a hydrazine group in the presence of an acid as catalyst. The condensation reaction can be carried out in the presence of hydrochloric acid as catalyst by heating eg., in isopropanol or toluene and possibly with a Dean-Stark apparatus. It can be advantageous in some instances to first transform the hemiketal into an isochromenilium salt of formula (IVa) with a mineral acid ag, perchloric acid and the latter is reacted with a hydrazine reagent, for example in isopropanol The thus-obtained hydrazones of formula (VII) are generally formed as a mixture of stereoisomers. They can be further reacted eg, with methanesulfonyl chloride in dichloromethane in the presence of triethylamine, and the mesylate obtained after isolation is treated with a concentrated solution of a base in an alcohol or a mixture of alcohol- dichloromethane. The ring closure reaction can be achieved for example, by the Mtsunobu reaction (Mitsunobu S+sis 1 : 1 (1981)) as well.

[0052] If desired, the compounds of formula (I) or (II) obtained by different methods can be transformed into other compounds of formula (I) or (II) with further reactions. For example, a reactive halogen atom in the side chain of the heterocycle-the R3 substituent--can be exchanged for an amino group, for example by heating with an excess of a proper amine, or the NH group of a N-containing heterocyclic compound can be alkylated by known methods.

The latter transformation for example in the case of a triazolyl compound, can be carried out with methyl iodide in the presence of potassium tert-butoxide.

[0053] The reduction of the nitro group in the compounds of formula (I) or (II) is generally carried out in polar solvents at room temperature or at elevated temperature in the presence of catalysts such as Raney-nickel, platinum or palladium. Besides gaseous hydrogen, other hydrogen sources ag, hydrazine hydrate, ammonium formate, potassium formate or cyclohexene can also be applied. The nitro group can be reduced, for example, with tin in the presence of an acid, or with tin (II) chloride by heating in an alcohol as well. The amino group can be further derivatised by known methods, for example alkylation, acylation, or Sandmeyer reaction.

[0054.] The ANTA antagonistic activity of the compounds of formula (I) or (II) of the present invention is exemplified bythe following experiments. Reference to compounds by number refers to compounds described in the numbered examples below.

Inhibition of the AMPA receptors [0055] Two experimental models were used for the demonstration of the inhibition of the AMPA receptor activation of the compounds of formula (I) or (II). In the first model the spreading depression caused by glutamate agonists (ie, AMPA or kainate) was studied, while in the second one the transmembrane ion-current induced bythe activation of the AMPA/kainate receptors was measured directly.

Inhibition of AMPA induced"spreading depression"in isolated chicken retina- [0056] The AMPA antagonistic effect of the compounds of formula (I) or (II) was studied in the mo"spreading depression"model (Sheardown Brain Res. 607:189 (1993)). The AMPA antagonists prolong the latency of the development of the"spreading depression"caused by AMPA (5 I1M).

Table 1 Inhibition of the"spreading depression"in chicken retina Compound (Number of example) / IC50 µM GYKI 52466 GYKI 53773 61 69 86 84 (reference) (reference) 9. 5 1.2 1-5 0. 9 0. 42 0.85 [00571 The data of Table 1 indicate that the compounds of the present invention inhibit the AMPA-induced"spreading depression"with an ICso value of 0.4-5 p. M.

Inhibition of AMPA induced transmembrane currents [0058] The activity of the compounds of the present invention was studied on acutely isolated cerebella Purk1nje cells by measuring the whole-cell current induced by 5 p. M AMPA according for example to the method described byBleakman et al, (Neuropharmacology 12 : 1689 (1996) ). According to the IQo values obtained, the compounds of the present invention inhibit the AMPA-induced ion-current by one to two magnitudes greater than the internationally accepted reference compound GYKI 52466 (5- (4-aminophenyl)-9H-1, 3-dioxolo [4,5-h] [2,3]- benzodiazepine, Hungarian patent No. 191 698), or GYKI 53773 ((R)-7-acetyl-5-(4- aminophenyl)-8, 9-dihydro-8-methyl-7H-1,3-dioxolo [4,5-h] [2,3] benzodiazepine, U. S. Patent No.

5, 536, 832), the IQo values of which are 8.8 µM, and 1.57 µM, respectively. (See Table 2).

Table 2.

Inhibition of the ion-currents caused by 5 RM ÅMPA detetmined bv the whole cell patch clamp method Compound (Number of example) / IC50 µM GYKI 52466 GYKI 53773. 61 69 86 84 (reference) (reference) 8. 8 1.57 0.49 0.42 0.06 0.09 A nticonulsant activity [0059] Although various drugs with different spectra of activity are used in the therapy of epilepsy, they show severe side effects. Furthermore, about 30% of epilepsy patients are refractoryto these drugs. Consequently, there is a need for such new antiepileptic drugs, which act via a mechanism different from those in current use. There are great expectations towards those compounds that displaytheir activity by diminishing the glutamate-induced over-activation of the central nervous system (TIPS, 15: 456 (1994)).

[0060] The anti-seizure activity of some of the compounds of the present invention was measured using the electroshock test (J. Pharmacol. Exp. Ther. 106: 319 (1952)) and the results are shown in Table 3. The spasmolytic activity of the compounds of the present invention was investigated byusing eg, pentetrazole (z. PhanmcoL Exp. Ther. 108: 168 (1953)), strychnine Pharmacol. Exp. Ther. 129: 75 (1960)), bemegrid, nicotine, bicuculline, 4-aminopyridine and mercapto-propionic acid for inducing the clonic-tonic seizures and lethality. The investigated compounds were administered orally in three doses using 10 male CD1 mice/dose, usually60 min before the induction of seizures. Non-limiting, illustrative results are summarized in Table 3.

Table 3 Investigation of the anticonvulsive activity in mice Compound (Number of example)/ED50 mg/kg po. Method GYKI GYKI 61 69 86 84 89 102 52466 53773 MES 37. 4 8. 6 13. 1 14. 7 6.1 12.5 10. 5 13.9 MES 30'21.9 4.9 11.5 8.7 4.3 10-15 Pentetrazol 119. 8 16.8 32.5 46.9 10.0 17.1 11. 5 35. 7 Strychnine 86. 7 17.4 35.4 27.7 10.6 18.2 15.7 26.7 Bemegride 71.9 23.9 34.4 33.3 11.2 16.7 11.2 27.9 Bicuculline 35.0 14.6 31.0 18.1 4.6 17.0 17. 1 25. 8 Nicotine 71.8 22. 7 59.3 16.8 16.5 77. 2 45. 9 31. 7 4-AP 43.0 8.4 17.6 16.6 10.1 16.6 14.3 20.4 3-MPA 47. 0 17.1 11. 0 342 4. 0 6. 8>50>50 Abbreviations : MES = maximal electroshock seizure ; 4-AP = 4-aminopyridine ; 3-MPA = 3-mercapto-propionic acid [0061] The data provided above indicate that the compounds of formula (I) or (II) of the present invention showed significant anticonvulsive activity all of the eight tests studied.

They reveal both a broader spectrum and more significant anticonvulsive efficacy compared to GYKI 52466 and GYKI 53773, both used as reference compounds in the literature. The protective effect displayed against the different convulsion inducing agents predicts favorably for their potential use in the treatment of the different kinds of epilepsy.

Muscle relaxant activity [0062] Central muscle relaxants are used in such clinical situations when the resting tone of the skeletal muscles is increased as a consequence of a cerebral trauma or due to a chronic neurodegenerative illness, resulting in muscle rigidity or tremor. The muscle spasm is often painful and hinders normal motion.

[0063] The muscle relaxant activity of the compounds of formula (I) or (II) of the present invention was determined in the inclined screen test described byRandall (T. Pha Exp. T'her. 129 : 163 (1960)) as well as in the rotarod test (Dunham et aL, J. A m Phatm A ssoc.

46: 208 (1957)). The compounds were administered in three doses intraperitoneallyusing 10 1-mice/dose. The muscle relaxant activity of the compounds of the present invention was compared to that of the reference compounds GYKI 52466 and GYKI 53773. Representative, non-limiting results are summarized in Table 4. From these data, it is evident, that the muscle relaxant activity of the compounds of the present invention significantly exceeds that of GYKI 53773, which is now in clinical phase-II studies.

Table 4 Muscle relaxant activity in mice Compound Inclined screen Rotarod (Number of example) EDso ip. (mg/lig) EDso ip. (mg/kg) GYKI 52466 (reference) 47.1 25.1 EI 53773 (reference) 13.4 2.3 61 10.7 5. 4 69 12.2 1.2 86 3.9 0.8 84 12.8 1.4 89 4.3 1.7 102 14.8 2.9 [0064] The muscle relaxant activity of the compounds of formula (I) or (II) determined in the above tests indicates potential therapeutic use in the treatment of such illnesses in which the increased muscle tone causes problems. Considering their skeletal muscle relaxant and anti- tremor activity (discussed below), the compounds may be useful in the treatment of essential tremor, multiple sclerosis (spasms + tremor) and Parkinson's disease (rigidity + tremor).

The inhibition of focal ischemia [0065] The focal anti-ischemic activity of the compounds of formula (I) or (II) of the present invention was measured by the"middle cerebral artery occlusion" (MCAO) test (Battus Starke 11: 2265 (1994) and Sydserff et al., Brit. J. Pharmacol. 114:1631 (1995)). The blood supply of the left middle cerebral artery of anaesthetized rats was temporarily blocked (60 min) by an embolus introduced intra-arterially following Halothane anesthesia, without craniotomy, thereafter the perfusion was reestablislied by removing the embolus and thus a human"stroke- like"status was triggered in an experimental animal model. After a histological process (ITC staining) 24h later, the infarcted area was determined by a computer assisted scanner program and was compared to the results obtained in a control group treated with the vehicle. Non- limiting, representative results are summarized in Table 5.

Table 5.

Inhibition of focal ischemia in rats Compound Dose Decrease of the infracted area in % compared to (Number of mg/kg iv. that of the control example) (6x in every 30 min 120 min 180 min 30 mis Time of first treatment after occlusion GYKI 52466 HC1239 (reference) 5 34* 47** GYKI 53773 2 47-49-26 (reference) 61 1 63** 16 2 46" 69 2 28 86 1 35* # p <0. 05 ; 88 p <0.01 ; calculated with Dunnett test. following ANOVA (Dunnett J. Amer. Statist. Ass. 50: 1096 (1955)) [0066] The investigated compounds possess a strong neuroprotective activity this experimental model, which is considered the model of the human stroke. Some of the compounds, ag, those described in Example 61 and 86, show significant activity even when administered 3 h after the occlusion predicting a potential useful clinical application.

Inhibition of autoimmune inflammation [0067] Multiple sclerosis is a chronic autoimmune inflammation of the central nervous system in which the axonal myelin coat, assuring the safe impulse conduction, is damaged. The oligodendrocytes forming the myelin coat express mainlyAMPA/kainate receptors. Thus, the neurodegenerative process is further enhanced by glutamate, the excitatory neurotransmitter, which is released bythe activated immune cells in large quantities which expresses its activity through AMPA/kainate receptors thereby damaging myelin oligodendrocytes and axons of neurons (Steiman Nature Medicine 6.15 (2000) and Werner et al., J. Neurol. Transmiss. Suppl., 60 : 375 (2000) ). As a consequence of these processes, at first mild neurological symptoms, such as visual, sensory, balance, motion and urogenital problems develop which become increasingly serious. The therapy of multiple sclerosis is still an unsolved problem despite the intense research being pursued in this field (Bjartmar etat, gs qFTo*38 : 17 (2002)).

[0068] Muscle spasticity and intention tremor belong to the most severe neurological symptoms of multiple sclerosis (Baker et aL, Nature 404: 84 (2000) ). Moderation or cure of these symptoms by a proper therapy would be very important., [0069] The activity of the 2,3-benzodiazepine derivatives possessing AMPA antagonistic activity was further investigated in an autoimmune encephalomyelitis model (Smith et aL, Nature Medicine, 6 : 62 (2000) ) in rats, using immunization with guinea pig myelin basic protein (MBP) and complete Freund adjuvant. The compounds were administered intraperitoneallytwice a day for 8 days, starting on day 10 after immunization and with an observation period until symptoms were present. 5-15 animals were used in each group. Their weights were 160-180 g (Lewis rats, female) and 180-220 g (Lewis rats, male). The activity of the compounds was determined according to the symptom score values, and compared to those of the control group (see Table 6). Histopathological investigations were carried out on the brain stem, the spinal cord, and the sciatic nerve (Gijbels etal, J. Clin I ? mt. 94: 2177 (1994) ) using 5-10 animals/group. Non- limiting, representative results are presented in Table 7.

Table 6.

Effect of 2, 3-benzodiazepines possessing AMPA antagonist activity on the clinical symptoms of autoimmune encephalomyelitis in Lewis rats Neurological symptoms Compound Dose (change compared to controls, %) (Number of (mg/kg ip. ) Female rats Male rats example) 0-8 day 0-14 day 0-8 day 0-14 day GYKI 53773 30-38"-27-43"-29 (reference) 15 -60* -63** -8 +7 GYKI 52466 30-45-4-1-1 (reference) 86 15 -97** -85** -93* -67 7. 5 -62** -66** -65** -70** 3. 75 -3 -18 -70** -77** 1. 875-40""'-39'-'+5-8 61 7. 5-56"-53"-60"-63"" 3. 75 -44 -48 -44* -46* 1.875-18-7 +13 +5 69 7. 5 -29 -24 -51* -50* 3.75 +43 +58"+35-40" # p <0.05; ** p <0.01 (Mann-Whitney test) Table 7.

Effect of 2 3-benzodiazepine derivatives possessing AMPA antagonistic character on the histological and clinical symptoms of autoimmune encephalomyelitis in Lewis rats on day 24 after immunization. Histological symptoms Neurological symptoms Compound Dose (change, %) (change, %) (Number of (mg/kg ip.) rats rats example) Male female male female GYKI 53773 30 +34-16-26-41 (reference) 86 15-66-53-67-85 7.5 +1-22-66-62 3. 75 +4-20-72-21 1. 875-25-15 +54-42 61 7.5-20-5-54-53 [0070] According to our histopathological and pharmacological investigations the compounds described in, for example, Example 86 and 61 proved to be more active than the reference compound GYKI 53773.

[0071] The anti-tremor effect of the 2, 3-benzodiazepine derivatives of the present invention, possessing AMPA antagonistic character in mouse models was studied using three tremoligen agents of different mechanism of action, such as oxotremorine (Rathbun et al., Psychopharmacology, 4: 114 (1963)), GYKI 20039 (3- (2, 6-dichlorophenyi)-2-imino-thiazolidine ; (Andrási et al., Acta Physiol, Acad. Sci. Hung. 37 : 183 (1970)) and harmaline. Number of animals: 5/group. Weight of animals: 20-25 g (GDI mice, male). The activity of the investigated compounds was determined bytheir score values compared to those of the control group. The ED50 values were calculated according to the Litchfield-Wjlcoxon method and are listed in Table 8.

Table 8.

Effect of 2, 3-benzodiazepine derivatives possessing AMPA antagonistic character on the tremor of CD1 mice induced by different chemical agents. Compound Dose range EDso (mg/kg po.) (Number of (mg/kg p. o.) Oxotremorin GYKI 20039 Harmaline example) 1 mg/kg ip. 10 mg/kg ip. 40 mg/kg ip. GYKI 52466 6.25-75. 0 20.5 (14.9-28. 3) 37.1 (25.2-54. 7) 38.5 (25.7-57. 9) (reference) GYKI 53773 3.125-20. 0 5.6 (3. 6-8. 5) 10.6 (7.2-15. 5) 9.0 (-7. 4-10. 9) (reference) 86 3.125-9. 0 4.3 (3.5-5. 4) 6.8 (5.5-8. 5) 6.0 (4. 9-7. 4) [0073]'According to our investigations, the compound described in Example 86 was more active than the reference compounds GYKI 53773 and GYKI 52466, respectively.

[0074] The 2,3-benzodiazepine derivatives with AMPA antagonistic character, compensating for the harmful effect of glutamate by blocking the corresponding receptors, are therapeutically important. Their combined neuroprotective, muscle relaxant, tremor inhibiting etc. properties beneficially influence the progression of the pathological neurological disorders and diminish the pathological neurological symptoms, respectively. <BR> <BR> <BR> <P>IDe @ect of the compounds of the present invention on the acute and chronic inflammatory disorders of the air@@@ Bronchial hyperresponsiveness (BHR) and airway eosinophilia (AEP) are characteristic features of bronchial asthma. BHR is typified by an exaggerated response to a wide variety of stimuli that can induce an increased resistance to airflow in the airways. AEP is a result of prolonged eosinophil infiltration, mast cell, and T cell activation in the airways. In actively (ego ovalbumin) immunized rats (eg, Brown Norway [BN] strain), repeated sensitization followed by antigenic challenge results in lung eosinophilia and bronchial hyperresponsiveness to different spasmogens (eg, acetylcholine). This is the most frequently employed model for studying potential anti- asthmatic effects of new chemical entities.

[0075] BN rats were activelyimmunized with allergen (ovalbumin). On day one, rats were sensitized with the subcutaneous administration of ovalbumin suspended in Al (OH) 3 (2.5 llg ovalbumin + 20 mg A1 (OH) 3 in 0.5 rnl saline). Booster injections (same dose and same route) were given at day 14 and 21. Simultaneously at each occasion 0.25 ml of Bordatella pertussis vaccine was injected intraperitoneally. On day28, animals were challenged byinhalation of the antigen (vaporized 1% OVA solution for 1 hour). Test compounds were administered orally2 hours pre-challenge.

[0076] 48 hours following challenge, theywere sacrificed by an overdose of urethane (4- 5 ml of 15% urethane given i. p. ), bronchoalveolar lavage fluid (BALF) was obtained, and tracheae dissected from the animals. Eosinophil cell count (cells/ml BALF) was determined manually using a selective stain for eosinophils and counting the cells in a Buerker chamber.

BHR was determined using tracheal rings suspended in an organ bath. After an equilibration period of 30 minutes, cumulative concentration response curves to acetylcholine were determined. Maximal response of control (unchallenged, non-treated) tracheal rings is obtained at 10-3 M acetylcholine. The height of this response is defined as 100%. All other contractions are expressed as a percentage and related to the control response.

Results Table 9. <BR> <BR> <BR> <BR> <BR> <P> Effect of GYKI 52466 (reference), GYKI 53773 (reference) and the compound described<BR> in Example 86 on the bronchial hypersensitivity and the eosinophilia of the airways on BN-rats sensitized with ovalbumin and antigen challenged by inhalation (meanSE, p determined by Student's t-test). Compound (Number of example) Experiment Parameter Control Challenge GYKI 52466 (reference) 3.0 mg/kg po ED50* 5.63~0.46 6.74~1. 45 5. 601. 53 1 p 0. 002 0. 028 MAX** 100~0 276~217 135~195 p 0. 001 0.037 Eosinophil*** 0.17~0. 01 1. 240. 23 0. 910. 13 p 0. 010 NS# Experiment Parameter Control Challenge GYKI 53773 (reference) 3. 0 mg/kg po ED50* 5.22~0. 59 5. 890. 66 4. 640. 91 p 0. 003 0. 001 1000 16365 8543 p <0. OO1 0.007 Eosinophil*** 0. 380. 11 1. 240. 13 1. 29~0.11 p 0. 004 NS# Experiment Parameter Control Challenge 86 3.0 mg/kg po 3 ED50* 5. 780. 17 6. 990. 32 4. 950. 59 p 0. 001 0. 008 MAX 1000 25550 8114 p 0. 001 0.003 Eosinophil*** 0. 230. 08 1. 430. 27 1. 320. 32 p 0.005 NS# acetylcholine (Ach) concentration (-log M) which causes a 50% contraction compare to the control relative contraction compared to the control at a maximal Ach concentration BALF eosmophil number (xlOVml) not significant (p 0. 05) [0077] The representative results presented in Table 9 show that representative compounds according to the present invention diminished the bronchial hyperresponsiveness caused bythe allergen. The eosinophilia was not significantlyinfluenced bythe applied doses.

[0078] The results of the different pharmacological investigations mentioned above show that the compounds of formula (I) or (II) of this invention are able to beneficially influence various diseases and disorders in which glutamate (AMPA/kainate) receptors have been Implicated. Consequently the compounds according to the invention are suitable for treating neurological and psychiatric disorders, triggered by the extremely enhanced activity of the AMPA receptor. Therefore, they have therapeutic utilityas anticonvulsants, muscle relaxants, as well as neuroprotective agents. They also display therapeutic value for the treatment of epilepsy, as well as different illnesses in which the spasm of skeletal-muscles is involved, and in the treatment of neurodegenerative disorders such as ag, cerebral ischemia (stroke).

[0079] Exemplary neurological illnesses which can be beneficially influenced or prevented include Parkinson's disease, Alzheimer's disease, Huntington choreag amyotrophic lateral sclerosis, olivopontocerebellaric atrophy, AIDS dementia, senile dementia. A similar beneficial effect can be achieved in the treatment of neurodegenerative processes caused by cerebrovascular catastrophe (stroke, brain, and spinal injuries) or hypoxia, anoxia or hypoglycemia. The compounds of the invention can be advantageously used for the treatment of different psychiatric diseases such as anxiety, schizophrenia, sleep disorders, as well as alleviating the withdrawal syndrome of alcohol and drug abuse. Furthermore they may inhibit tolerance development in the case of sedatives or analgesics.

[0080] It can be expected that they can be advantageously used in epileptic disease entities, in the cure or palliation of muscle spasms of central origin and in the relief of pathologic pain as well as in the treatment of urinary incontinence.

[0081] In one aspect of the invention, a method of blocking the activation of one or more excitatory amino acid receptors in mammals is provided. This method includes administering to a mammal in need of such treatment a pharmaceuticallyeffective amount of a compound of the formula (I) or (II).

[0082] In another aspect of the invention, a method of treating epilepsy In mammals is provided. This method includes administering to a mammal in need of such treatment an antiepileptic amount of a compound of the formula (I) or (II).

[0083] In another aspect of the invention, a method of treating spasms of the skeletal musculature in mammals is provided. This method includes administering to a mammal in need of such treatment a muscle-relaxing amount of a compound of the formula (I) or (II).

[0084] In still another aspect of the invention, a method of treating acute and chronic neurodegenerative disorders in mammals is provided. This method includes administering to a mammal in need of such treatment a pharmaceutically effective amount of a compound of the formula (I) or (II).

[0085] In yet another aspect of the invention, a method for treating inflammatory disorders in mammals is provided. This method includes administering to a mammal in need of such treatment a pharmaceutically effective amount of a compound of the formula (I) or (II).

[0086] In other aspects of the invention, the compounds of formula (I) or (II) can be advantageously used in the treatment of multiple sclerosis. A further therapeutic field, in which the compounds of formula (I) or (II) can be used, are illnesses that are caused by the over- function of the periferic glutamate receptors. Such illnesses include the acute and chronic inflammatory disorders of the airways particularly allergic inflammations such as asthma-related pathologies. This latter potential therapeutic use is supported by the results obtained in ovalbumin sensitized rats.

[0087] In one aspect of the invention, a pharmaceutical composition is provided including a compound of formula (I) or (II), or a stereoisomer, or a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable carrier, excipient or diluent.

[0088] The compounds of formula (I) or (II) are formulated in a pharmaceutically acceptable vehicle with any of the well-known pharmaceutically acceptable carriers, including diluents and excipients (see Remington's Pharmaceutical Sciences, 18th Ed. , Gennaro, Mack Publishing Co. , Easton, PA 1990 and Remington : The Science and Practice of Pharmacy.

Lippincott, Williams &Wlkins, 1995). While the type of pharmaceutically acceptable carrier/vehicle employed in generating the compositions of the invention will vary depending upon the mode of administration of the composition to a mammal, generallypharmaceutically acceptable carriers are physiologicallyinert and non-toxic. Formulations of pharmaceutical compositions may contain more than one type of compound of formula (I) or (II), as well as any other pharmacologically active ingredient useful for the treatment of the particular conditions, disease, or symptom being treated.

[0089] The compositions of the invention can be administered by standard routes (ag, oral, inhalation, rectal, nasal, topical, including buccal and sublingual, or parenteral, including subcutaneous, intramuscular, intravenous, intradermal, transdermal, and intratracheal). In addition, polymers may be added according to standard methodologies in the art for sustained release of a given compound.

[0090] For oral administration, the compositions of the invention maybe presented as discrete units such as capsules, caplets, gelcaps, cachets, pills, or tablets each containing a predetermined amount of the active ingredient as a powder or granules; as a solution or a suspension in an aqueous liquid or a non-aqueous liquid; or as an oil-in-water liquid emulsion or a water-in-oil emulsion and as a bolus, etc Alternately, administration of a composition including the compound of formula (I) or (II) maybe effected by liquid solutions, suspensions or elixirs, powders, lozenges, micronized particles and osmotic delivery systems.

[0091] Formulations suitable for administration by inhalation include formulations that can be dispensed byinhalation devices known to those in the art. Such formulations may include carriers such as powder and aerosols. Liquid and powdered compositions suitable for nebulization and intrabronchial use, or aerosol compositions administered via an aerosol unit dispensing tnetered doses ("MDI") are contemplated.

[0092] The active ingredient may be formulated in an aqueous pharmaceutically acceptable inhalant vehicle, such as, for example, isotonic saline or bacterostatic water and other types of vehicles that are well known in the art. The solutions are administered by means of a pump or squeeze-actuated nebulized spray dispenser, or by any other conventional means for causing or enabling the requisite dosage amount of the liquid composition to be inhaled into the patient's lungs.

[0093] Powder compositions include, byway of illustration, pharmaceutically acceptable powdered preparations of the active ingredient thoroughly intermixed with lactose or other inert powders acceptable for intrabronchial administration. The powder compositions can be administered via a dispenser, including, but not limited to, an aerosol dispenser or encased in a breakable capsule, which maybe inserted by the patient into a device that punctures the capsule and blows the powder out in a steady stream.

[0094] Aerosol formulations for use in the subject method typically include propellants, surfactants, and co-solvents and maybe filled into conventional aerosol containers that are closed by a suitable metering valve.

[0095] Formulations suitable for nasal administration, wherein the carrier is a solid, include a coarse powder having a particle size, for example, in the range of 20 to 500 microns which is administered in the manner in which snuff is administered, ive by rapid inhalation through the nasal passage from a container of the powder held close up to the nose. Suitable formulations, wherein the carrier is a liquid, for administration, for example via a nasal spray, aerosol, or as nasal drops, include aqueous or oily solutions of the compound of formula (I) or (II).

[0096] Formulations suitable for parenteral administration include aqueous and non- aqueous sterile injection solutions which may contain antioxidants, stabilizers, buffers, bacteriostats and solutes which render the formulation isotonic with the blood of the intended recipient; and aqueous and non-aqueous sterile suspensions which may include suspending agents and thickening agents.

[0097] The dosage of the active ingredient depends on the route of administration, the type and severity of the disease as well as the weight and age of the patient. The daily dose for adult patients can be 0.1-500 mg, preferably 1-100 mg, in a single dose or divided in several doses.

[0098] In another aspect of the present invention, a method is provided for treating (a) an acute or chronic neurodegenerative disease associated with glutamate dysfunction; (b) a method for treating epilepsy ; (c) a method for reducing muscle spasm in mammals; (d) a method for preventing, treating or alleviating the symptoms of acute or chronic inflammatory disorders of the airways ; (e) a method for relief of pathological pain in mammals. These methods include administering to a mammal in need of such treatment a therapeutically effective amount of a compound of formula (I) or (II).

[0099] The term"therapeutically effective amount is used to denote treatments at dosages effective to achieve the therapeutic result sought. Furthermore, one of skill will appreciate that the therapeutically effective amount of the compound of the invention may be lowered or increased by fine-tuning and/or by administering more than one compound of the invention, or by administering a compound of the invention with another pharmacologically active compound. The invention therefore provides a method to tailor the administration/treatment to the particular exigencies specific to a given mammal. As illustrated in the following examples, therapeutically effective amounts may be easily determined for example empirically by starting at relatively low amounts and by step-wise increments with concurrent evaluation of beneficial effect.

[00100] It will be appreciated by those of skill in the art that the number of administrations of the compounds according to the invention will vary from patient to patient based on the particular medical status of that patient at any given time.

[00101] The compounds according to the invention and the process for their preparation are illustrated in detail by the following Examples.

[00102] The following examples are intended to further illustrate certain preferred embodiments of the invention and are not limiting in nature. Those skilled in the art will recognize, or be able to ascertain, using no more than routine experimentation, numerous equivalents to the specific substances and procedures described herein.

EXAMPLES [00103] The starting materials of the examples were synthesized as follows: (~)-8-Methyl-5-(4-nitrophenyl)-7-thiocarbamoyl-8,9-dihydro-7 H-1,3-dioxolo[4 5- h][2,3]benzodiazepine (I) [00104] A mixture of 0.90 g (9.26 mmol) of potassium thiocyanate, 2.00 g (6.15 mmol) of (~)-8-methyl-5-(4-nitrophenyl)-8,9-dihydro-7H-1, 3-dioxolo [4,5-h] [2,3] benzodiazepine and 40 ml of acetic acid was stirred at 100-110°C for 6 h. After cooling, the precipitated crystals were filtered off, washed with water and dried to yield 1.80 g (76%) of the title compound. Mp.: 242- 243°C.

[00105] The thiocarbamoyl compounds II-X were synthesized from the corresponding dihydro-[2, 3] benzodiazepine according to the above procedure.

(R)-8-Methyl-5-(4-nitrophenyl)-7-thiocarbamoyl-8,9-dihydr o-7H-1,3-dioxolo[4, 5- h][2,3]benzodiazepine (II) [00106] Mp.: 213-215 °C. Yield: 73 %, [α] D :-251° (c=0. 5; CHCl3).

(S)-8-Methyl-5-(4-nitrophenyl)-7-thiocarbamoyl-8,9-dihydr o-7H-1,3-dioxolo[4,5- h] [2, 3] benzodiazepine (III) [00107] Mp.: 213-214 °C. Yield: 76 %, [a] : +252° (c=1 ; CHCl3).

(~)-8-Methyl-5-(3-methyl-4-nitrophenyl)-7-thiocarbaomyl-8 ,9-dihydro-7H-1,3-dioxolo[4,5- h] [2 ! 3] benzodiazepine (IV) [00108] Mp.: 230-236 °C. Yield: 86 %.

(~)-8-Chloro-4-methyl-(4-nitrophenyl)-3-thiocarbamoyl-4,5 -dihydro-3H-[2,3]benzodiazepine (V) [00109] Mp.: 261-265°C. Yield: 72%.

(-)-7, 8-Dichloro-4-methyl-1-(4-nitrophenyl)-3-thiocarbamoyl-4,5-di hydro-3H- [2, 3] benzodiazepine, 1 [00110] Mp. : amorphous. Yield: 59%. <BR> <BR> <BR> <BR> <BR> <P>(#)-8-Methyl-5-phenyl-7-thiocarbonyl-8,9-dihydro-7H -1,3-dioxolo[4,5-h][2,3]benzodiazepine (VII) [00111] Mp.: 225-235°C. Yield: 86%.

5-(4-Nitrophenyl)-7-thiocarbamoyl-8,9-dihydro-7H-dioxolo[ 4,5-h][2,3]benzodiazepine (VIII) [00112] Mp.: 235-238°C. Yield: 62%.

(~)-8-Methyl-5-(4-methyl-3-nitrophenyl)-7-thiocarbamoyl-8 ,9-dihydro-7H-1,3-dioxolo[4, 5- h] 6233benzodiazepine (IX) [00113] Mp.: 201-202°C. Yield: 84%.

(~)-7-Bromo-4-methyl-8-methoxy-1-(4-nitrophenyl)-3-thioca rbamoyl-3,4-dihydro-3H- [2. 3] benzodiazepine (X) [00114] Mp.: 250-253°C. Yield: 94%.

(~)-8-Methyl-5-(4-nitrophenyl)-8,9-dihydro-7H-1,3-dioxolo [4,5-h][2,3]benzodiazepine-7- carbothioyl chloride [00115] 3.25 g (10.0 mmol) of ()-8-methyl-8, 9-dihydro-7H-1,3-dioxolo [4,5- h] [2,3] benzodiazepine was dissolved in 90 ml of drytoluene bywanning and after adding 2.17 ml (15.5 mmol) of triethylamine, was reacted with 1.90 rnl (15.0 mmol) of trimethylsilyl chloride at about 28-30°C. After stirring at room temperature for 16 h this reaction mixture was added dropwise over a period of about 2 h to the solution of 1.38 g (12.0 mmol) of thiophosgene in 30 ml of dry toluene. This mixture was stirred at room temperature for 5 h, and then diluted with 30 ml of toluene. It was then decomposed by addition of 30 ml of water. After separation, the toluene phase was washed twice with 30 ml of water, followed by a 10% aqueous sodium chloride solution. After drying, the solvent was evaporated and the residue was treated with diisopropyl ether to yield 3.27 g (81%) of the crude product.

[00116] The crude product was recrystallized from chloroform, petroleum ether.

[00117] Yield: 3.05 g. Mp.: about 185°C it recrystallizes, then it melts at 210°C.

[00118] The carbothioyl chloride type compounds XII-XVII were synthesized by analogous methods from racemic or optically active dihydro- [2, 3] benzodiazepine derivatives: (R)-8-Methyl-5-94-nitrophenyl)-8,9-dihydro-7H-1,3-dioxolo[4, 5-h][2,3]benzodiazepine-7- carbothioyl chloride (XII) [00119] Mp.: 187-188°C. Yield: 80%, [α] D :-610° (c=0.5 ; Chai3).

(~)-8-Methyl-5-(3-nitrophenyl)-8,9-dihydro-7H-1,3-dioxolo [4,5-h][2,3]benzodiazepine-70 carbothioyl chloride (XIII) [00120] Mp.: 198-199°C. Yield: 79%.

(~)-8-Methyl-5-(3-methyl-4-nitrophenyl)-8,9-dihydro-7H-1, 3-dioxolo[4,5-h][2,3]benzodiawzepine- 7-carbothioyl chloride [00121] Mp.: 210-215 °C. Yield: 79 %.

(~)-8-Methyl-5-(4-methyl-3-nitrophenyl)-8,9-dihydro-7H-1, 3-dioxolo[4,5-h] [2,3]benzodiazepine- 7-carbothio@ chloride [00122] Mp.: 201-202 OC Yield: 84 %.

(~)-8-Chloro-4-methyl-1-(4-nitrophenyl)-4,5-dihydro-3H-[2 ,3]benzodiazepine-3-carbothioyl chloride [00123] Mp.: 210-214°C (DMF). Yield: 70%.

(~)-7-Bromo-4-methyl-8-methoxy-1-(4-nitrophenyl)-4,5-dihy dro-3H-[2,3]benzodiawzepine-3- carbothioyl chloride (DII ? [00124] Mp.: 199-204°C. Yield: 82%.

(~)-8-Methyl-5-(4-nitrophenyl)-8,9-dihydro-7H-1,3-dioxolo [4,5-h][2,3]benzodiawzepine-7- carbothiohydrazide (XVIII) [00125] l. Og (2.47 mmol) of carbothioyl chloride XI was added to a stirred solution of 0.37 g (7. 42 mmol) of hydrazine hydrate in 15 ml of tetrahydrofuran at 5-10 °C over a period of about 0.5 h, then after 1 h stirring, the mixture was poured into water and the precipitated product was filtered off to yield 0.89 g (90 %) of the crude product. After drying, it was used in the further reaction steps. The melting point of the product after recrystallization from ethanol was 196 °C.

[00126] The carbothiohydrazide derivatives X-XEI were synthesized by analogous methods: (R)-8-Methyl-5-(4-nitrophenyl)-8,9-dihydro-7H-1,3-dioxolo[4, 5-h][2,3]benzodiazepine-7- carbothiohydrazide (XIX) [00127] Mp.: 140-142 °C. Yield: 99 %, [cclD :-201° (c=0.5 ; CHC13).

(~)-8-Chloro-4-methyl-1-(4-nitrophenyl)-4,5-dihydro-3H-[2 ,3]benzodiazepine-3- carbothiohydrazide (XX) [00128] Mp.: 210-211 °C. Yield: 61 %.

(~)-7-Bromo-4-methyl-8-methoxy-1-(4-nitrophenyl)-4,5-dihy dro-3H-[2,3]benzodiazepine-3- carbothiohydrazide XXI) [00129] Mp.: 196-201°C. Yield: 98%.

(~)-8-Methyl-5-(3-methyl-4-nitrophenyl)-8,9-dihydro-7H-1, 3-dioxolo[4,5-h][2, 3] benzodiazepine- 7-carbothiohidrazide (XXII) [00130] Mp.: 188-190°C Yield: 98%.

(~)-8-Methyl-5-(4-nitrophenyl)-8,9-dihydro-7H-1,3-dioxolo [4,5-h][2,3]benzodiazepine-7- carbonitrile (XXIII) [00131] A mixture of 3.25 g (10 mmol) of ()-8-methyl-5- (4-nitrophenyl)-8, 9-dihydro- 7H-1, 3-dioxolo [4,5-h] [2,3] benzodiazepine, 20 ml of dimethylformamide, 2.76 g (20 mmol) of potassium chloride and 1.80 g (17 mmol) of cyanogen bromide was stirred at room temperature for 20 h. After pouring into water, the precipitated crystals were filtered off, and washed with water to yield 3.34 g (95%) of the title compound, Mp.: 172-176 °C.

(~)-8-Methyl-5-(4-nitrophenyl)-8,9-dihydro-7H-1,3-dioxolo [4,5-h][2,3]benzodiazepine-7- carbamidoxime (XXIV) [00132] A mixture of 2.80 g (8.0 mmol) of compound XXIII, 30 ml of 2-methoxyethanol, 0.84 g (10 mmol) of sodium acetate and 0.60 g (8.8 mmol) of hydroxylamine hydrochloride was stirred for 0.5 h, then concentrated in vacuum. The residue was treated with water, the precipitated crystals were filtered off and washed with water to yield 3.05 g (100%) of the title compound, Mp.: 138-145°C.

(~)-8-Methyl-5-(4-nitrophenyl)-8,9-dihydro-7H-1,3-dioxolo [4,5-h][2,3]benzodiazepine-7- carboxylic acid (2-chloroethyl)-amide (XXV) [00133] A mixture of 1.0 g (3.07 mmol) of ()-8-methyl-5- (4-nitrophenyl)-8, 9-dihydro- 7H-1, 3-dioxolo [4,5-h] [2,3] benzodiazepine, 25 rnl of drydichloromethane and 0.62 g (5. 88 mmol) of 2-chloroethyl isocyanate was stirred at room temperature for 24 h, then concentrated. The residue was purified by refluxing in ethanol to yield 1.25 g (94%) of the title compound, Mp.: 222-223°C.

(~)-Phenyl (8-methyl-5-(4-nitrophenyl)-8,9-dihydro-7H-1,3-dioxolo [4,5-h][2,3]benzodiazepine- 3-carbothioyl)-carbamate (XXVI) [00134] 0.37 g (3.80 mmol) of potassium thiocyanate was dissolved in 8 rnl of acetone, then 0.48 ml (3.80 mmol) of phenyl chloroformate was added dropwise to the mixture at room temperature. The reaction mixture was stirred at room temperature for 0.5 h, then at 40°C for 0.25 h. Then the mixture was cooled with ice-water and a solution of 1.04 g (3.20 mmol) of ()- 8-methyl-5-(4-nitrophenyl)-8,9-dihydro-7H-1,3-dioxolo[4,5-h] [2, 3] benzodiazepine in 15 rnl of acetone was added dropwise over a period of 0.5 h. After stirring for 0.5 h the bulk of the solvent was evaporated and the residue was treated with water, the crystals were filtered and washed with water to yield 1.73 g, (90%) of the title compound. Mp. : 160°C.

(~)-1-Methyl-3-{8-methyl-5-(nitrophenyl)-8,9-dihydro-7H-1 ,3-dioxolo[4 5- h][2,3]benzodiazepine-7-carbothioyl}-urea (XXVII) [00135] 1.57 g (3.11 mmol) of compound XXVI was dissolved in 8 ml of dimethylformamide and 0.35 ml (4.04 mmol) of 40% aqueous methylamine solution was added dropwise to the ice cooled stirred solution. After stirring for 2 h the mixture was poured into water, the precipitated crystals were filtered off and washed with water to yield 1.56 g of the crude product, which was recrystallized from ethanol. Yield: 1.01 g (73%). Mp.: 192-193°C [00136] The compounds XXVIII and XXIX were synthesized analogously.

(~)-1-Cyclopropyl-3-{8-methyl-5-(4-nitrophenyl)-8,9-dihyd ro-7H-1,3-dioxolo[4,5- h][2,3]benzodiazepine-7-carbothioyl}-urea (XXVIII) [00137] Mp.: 281-283°C (ethyl acetate). Yield: 80% (~)-1-Ethyl-3-{8-methyl-5-(4-nitrophenyl)-8,9-dihydro-7H-1,3 -dioxolo[4, 5- hie2, 3] benzodiazepine-7-carbothioyl}-urea (XXX) [00138] Mp.: 176-177°C (methanol). Yield: 73%.

(+)-i-{8-Methyl-5-(4-nitrophenyl !-8 9-dihydro-7H-1. 3-dioxoloU4. 5-h] [2. 3] benzodiazepine-7- carbothioyl}-4-methyl-semicarbazide (XXX) [00139] To a stirred solution of 0.40 g (1.0 mmol) of compound XVIII in 15 ml of chloroform 0.07 ml (1.2 mmol) of methyl isocyanate was added. After 1 h the reaction mixture was washed with sodium hydrogen carbonate solution and water and after concentration the obtained solid material was purified by refluxing in ethanol. The desired product was 0.36 g, yield: 88%. Mp.: 200°C.

(R)-8-Methyl-5-(3-methyl-4-nitrophenyl)-8,9-dihydro-7H-1, 3-dioxolo[4,5-h] E2 3] benzodiazepine (XXXI) [00140] The title compound was prepared based on the procedures described in the literature (Ling et al., J. Chem Soc Perkin Trans. 1: 1423 (1995) ) and the British patent specification No. 2,311, 779.

[00141] Mp.: 159-160°C (ethanol). [a] D: +172° (c=1 ; CHC13).

(R)-7-(tert-Butoxycarbonyl)-8-methyl-5-(4-nitrophenyl)-8, 9-dihydro-7H-1,3-dioxolo[4 5- h][2,3]benzodiazepine (XXXII) [00142] The compound was prepared according to a synthesis described in literature (Anderson et al., J. Am Chem Soc 117: 12358 (1995)) with the exception that tert-butyl carbazate was used instead of acetic hydrazide.

[00143] Mp.: 168-169°C (isopropanol). [a] D :-444° (c=0. 6 ; CHCl3).

Example 1 (~)-8-Methyl-5-(4-nitrophenyl)-7-(2-thiazolyl)-8,9-dihydro-7 H-1,3-dioxolo[4, 5- h] [2, 3] benzodiazepitie [00144] A mixture of 1.00 g (2.60 mmol) of the starting material I, 2.54 g (12.89 mmol) of bromoacetaldehyde diethyl acetal and 10 rnl of dimethylformamide was stirred at 80°C for 40 min. Then the reaction mixture was diluted with water and the crude product obtained was recrystallized from ethanol to yield 0.85 g (80%) of the title compound. Mp.: 145-150°C.

Example 2 <BR> <BR> <BR> <BR> (R)-8-Methyl-5-(4-nitrophenyl)-7-(2-thiazolyl)-8,9-dihydro-7 H-1,3-dioxolo[4, 5-<BR> <BR> <BR> h] [2. 3] benzodiazepine [00145] The title compound was obtained from the starting material II according to the method described in Example 1. Mp.: 108-110°C, yield: 89%, [a] D : +514° (c=0.5 ; CHCl3) Example 3 (S)-8-Methyl-5-(4-nitrophenyl)-7-(2-thiazolyl)-8,9-dihydro-7 H-1,3-dioxolo[4, 5- h] [2 3] benzodiazepine [00146] The title compound was obtained from the starting material III according to the method described in Example 1. Mp.: 114-116°C, yield: 83%, [a] :-522° (c=0.6 ; CHC13) Example 4 (~)-8-Methyl-7-(4-methyl-thiazol-2-yl)-5-(4-nitrophenyl)-8,9 -dihydro-7H-1,3-dioxolo[4, 5- h] [2, 3] benzodiazepine [00147] A mixture of 0.76 g (1.98 mmol) of the starting material I, 1.10 g (11.88 mmol) of chloroacetone and 15 ml of dimethylformamide was stirred at 80-90°C for 40 min. Then the reaction mixture was diluted-with water, the precipitated crystals were filtered off, dried and purified byrefluxing in ethanol to yield 0.69 g (82%) of the title compound; Mp.: 188-189°C.

Example 5 <BR> <BR> <BR> <BR> <BR> (~)-8-Methyl-7-(5-methyl-thiazol-2-yl)-5-(4-nitrophenyl)-8,9 -dihydro-7H-1,3-dioxolo[4, 5-<BR> <BR> <BR> h] [2, 3] benzodiazepine [00149] A mixture of 1.50 g (3.90 mmol) of starting material I, 3.57 g (19.50 mmol) of 2- bromopropionaldehyde dimethyl acetal and 15 ml of dimethylformamide was stirred at 90°C for 1.5 h. Then the reaction mixture was diluted with water and the crude product obtained was purified by column chromatography using silica gel (MN Kieselgel 60; Macherey-Nagel, Diiten, Germany) as adsorbent and a mixture of toluene-ethyl acetate (16: 1) as eluent to yield 1.08 g (66%) of the title compound; Mp.: 193-195°C.

Example 6 (~)-7-(4,5-Dimethyl-thiazol-2-yl)-8-methyl-5-(4-nitrophenyl) -8,9-dihydro-7H-1,3- dioxolo [4, 5-h] [2, 3] benzodiazepine [00150] A mixture of 0.60 g (1.56 mmol) of the starting material 1, 1.02 g (9.57 mmol) of 3-chloro-2-butanone and 8 ml of dimethylformamide was stirred at 90°C for 3 h. After cooling the precipitated crystals were filtered off, dried and purified by recrystallization from dimethylformamide and water to yield 0.49 g (76%) of the title compound; Mp.: >260 °C (dec. ).

Example 7 ()-8-Methyl-5- (4-nitrophenyl)-7- (4-phenyl-thiazol-2-yl)-8, 9-dihydro-7H-1, 3-dioxolo [4, 5- h] [23] benzodiazepine [00151] A mixture of 0. 45 g (1.17 mmol) of the starting material I, 0. 35 g (1.76 mmol) of phenacyi bromide and 7 ml of dimethylformamide was stirred at 80°C for 30 min. After cooling the precipitated crystals were filtered off, washed with ethanol and dried to yield 0.50 g (88%) of the title compound; Mp.: >260 °C (dec. ).

Example 8 <BR> <BR> <BR> <BR> (+)-7-4-Ethoxycarbonyl-thiazol-2-yl-8-methyl-5- (4-nitrophenyl-8, 9-dihydro-7H-1, 3-<BR> <BR> <BR> dioxolo 4, 5-h] [2, 3] benzodiazepine [00152] A mixture of 0.45 g (1.17 mmol) of the starting material I, 0.46 g (2.36 mmol) of ethyl bromopyruvate and 7 ml of dimethylformamide was stirred at 80°C for 30 min. After cooling the precipitated crystals were filtered off, washed with ethanol and dried to yield 0.41 g (85%) of the title compound; Mp.: 242-243°C Example 9 <BR> <BR> <BR> <BR> ()-7- (4, 5-Dihydro-thiazol-2-yl)-8-methyl-5-(4-nitrophenyl)-8,9-dihyd ro-7H-1,3-<BR> )-8,9-dih dioxolo [4, 5-h] [2, 3] benzodiazepine [00153] A mixture of 1.00 g (2.6 mmol) of the starting material I, 2.13 g (10.40 mmol) of 2-bromoethylamine hydrobromide and 10 ml of dimethylformamide was stirred at 90-100°C for 4 h. After diluting with water the precipitated crystals were filtered off, dissolved in dichloromethane and washed several times with 10% sodium hydrogen carbonate solution.

After drying the product was purified by column chromatography using silica gel (MN Kieselgel 60) as adsorbent and a mixture of hexane-ethyl acetate (1: 1) as eluent to yield 0.80 g (75%) of the title compound; Mp.: 185-187°C.

Example 10 <BR> <BR> <BR> <BR> (R)-7-(4,5-Dihydro-thiazol-2-yl)-8-methyl-5-(4-nitrophenyl)- 8,9-dihydro-7H-1, 3-<BR> )-8,9-dih<BR> dioxolo [4, 5-hl [2, 3] benzodiazepine [00154] The title compound was obtained from the starting material II according to the method described in Example 9.

[00155] Mp.: 118-124°C. Yield: 73%, [a] D : +575° (c=0.4 ; CHCl3).

Example 11 (S)-7-(4,5-Dihydro-thiazol-2-yl)-8-methyl-5-(4-nitrophenyl)- 8,9-dihydro-7H-1,3- dioxolo[4,5-h][2,3]benzodiazepine [00156] The title compound was obtained from the starting material III according to the method described in Example 9.

[00157] Mp.: 120-125°C. Yield: 71%. [a] D :-594° (c=0. 4; CHCl).

Example 12 (~)-7-(4,5-Dihydro-4-oxo-thiazol-2-yl)-8-methyl-5-(4-nitroph enyl)-8,9-dihydro-7H-1,3- dioxolo 4, 5-h] [2, 3 benzodiazepine [00158] A mixture of 1.00 g (2.6 mmol) of the starting material I, 1.19 g (7. 78 mmol) of methyl bromoacetate and 10 ml of dimethylformamide was stirred at 80-90°C for 1 h. After diluting with water the obtained crude product was purified by refluxing in methanol to yield 1.00 g (91%) of the title compound; Mp.: 218-220°C.

Example 13 <BR> <BR> <BR> <BR> <BR> ()-7- (4, 5-Dihydro-5-methyl-4-oxo-thiazol-2-yl)-8-methyl-5- (4-nitrophenyl)-8, 9-dihydro-<BR> <BR> <BR> <BR> 7H-1, 3-dioxolo [4, 5-h] [2. 3Jbenzodiazepine [00159] A mixture of 1.00 g (2.60 mmol) of the starting material I, 0.94 g (5.19 mmol) of ethyl 2-bromopropionate and 10 ml of dimethylformamide was stirred at 80-90°C for 2 h. After diluting with water the obtained crude product was purified by refluxing in 15 ml of ethanol to yield 1.08 g (95%) of the title compound; Mp.: 213-214°C.

Example 14 <BR> <BR> <BR> <BR> <BR> ()-7- (5, 6-Dihydro-4-oxo-4H-1, 3-thiazin-2-yl-8-methyl-5- (4-nitrophenyl)-8, 9-dihydro-<BR> <BR> <BR> 7H-1. 3-dioxolo [4, 5-h] [2, 31benzodiazepine [00160] A mixture of 2.00 g (5.20 mmol) of the starting material 1, 1. 89 g (10.44 mmol) of ethyl 3-bromopropionate and 20 ml of dimethylformamide was stirred at 80-90°C for 3 h. The reaction mixture was diluted with 25% sodium chloride solution and extracted with dichloromethane. After drying and concentration the crude product was purified by column chromatography using silica gel (MN Kieselgel 60) as adsorbent and a mixture of ethyl acetate- methanol (2: 1) as eluent to yield 1. 34 g (59%) of the title compound; Mp.: 220-221°C Example 15 5-(4-Nitrophenyl)-7-(2-thiazolyl)-8,9-dihydro-7H-1,3-dioxolo [4,5-h][2,3]benzodiazepine [00161] The title compound was obtained from the starting material VIII and bromoacetaldehyde diethyl acetal according to the method described in Example 1. Mp.: 203- 215°C Yield: 77%.

Example 16 <BR> <BR> <BR> <BR> (~)-8-Methyl-5-(3-methyl-4-nitrophenyl)-7-(2-thiazolyl)-8,9- dihydro-7H-1,3-dioxolo[4, 5-<BR> <BR> h] 3] benzodiazepine [00162] The title compound was obtained from the starting material IV according to the method described in Example 1. Mp.: 171-175°C. Yield: 46%.

Example 17 (~)-8-Methyl-5-phenyl-7-(2-thiazolyl)-8,9-dihydro-7H-1,3-dio xolo[4, 5- h] [2, 3] benzodiazepine [00163] The title compound was obtained from the starting material VII according to the method described in Example 1. Mp.: 180-184°C. Yield: 51%.

Example 18 <BR> <BR> <BR> <BR> (~)-7-Bromo-4-methyl-8-methoxy-1-(4-nitrophenyl)-3-(2-thiazo lyl)-4,5-dihydro-3H-<BR> <BR> [2, benzodiazepine [00164] The title compound was obtained from the starting material X according to the method described in Example 1. Mp.: 184-190°C Yield: 54%.

Example 19 <BR> <BR> <BR> <BR> ()-8-Chloro-4-methyl-1- (4-nitrophenyl)-3- (2-thiazolyl-4, 5-dihydro-3H-<BR> <BR> [2, 3 benzodiazepine [00165] The title compound was obtained from the starting material V according to the method described in Example 1. Mp.: 213-216°C. Yield: 67%.

Example 20 <BR> <BR> <BR> <BR> (~)-8-Chloro-4-methyl-3-(4-methyl-thiazol-2-yl)-1-(4-nitroph enyl)-4,5-dihydro-3H-<BR> <BR> [2, 31benzodiazepine [00166] The title compound was obtained from the starting material V according to the method described in Example 4. Mp.: 209-216°C. Yield: 94%.

Example 21 (~)-3-(4,5-Dihydro-thiazol-2-yl)-8-chloro-4-methyl-1-(4-nitr ophenyl)-4,5-dihydro-3H- [2, 3] benzodiazepine [00167] The title compound was obtained from the starting material V according to the method described in Example 9. Mp.: 225-227°C. Yield: 69%.

Example 22 (~)-3-(4,5-Dihydro-3-oxo-thiazol-2-yl)-8-chloro-4-methyl-1-( 4-nitrophenyl)-4,5-dihydro- 3H- [2, 3] benzodiazepine [00168] The title compound was obtained from the starting material V according to the method described in Example 12. Mp.: 226-228°C. Yield: 96%.

Example 23 <BR> <BR> <BR> <BR> ()-7, 8-Dichloro-4-methyl-3- (4-methyl-thiazol-2-yl)-1- (4-nitrophenyl)-4, 5-dihydro-3H- [2, 3] benzodiazepine [00169] The title compound was obtained from the starting material VI according to the method described in Example 4. Mp.: 240-242°C. Yield: 77%.

Example 24 (~)-7-(4,5-Dihydro-oxazol-2-yl)-8-methyl-5-(4-nitrophenyl)-8 ,9-dihydro-7H-1,3- dioxolo [4, 5-hl [2 3] benzodiazepine [00170] A mixture of 1.43 g (3.32 mmol) of the starting material XXV, 1.38 g (9.98 mmol) of anhydrous potassium carbonate, 0.24 g (1.60 mmol) of sodium iodide and 24 ml of dimethylfoimamide was stirred at 100-110°C for 4 h. Then the mixture was diluted with water and the precipitated crude product was recrystallized from ethanol to yield 1.00 g (76%) of the title compound; Mp.: 194-196°C Example 25 (~)-8-Methyl-5-(4-nitrophenyl)-7-(1,3,4-thiadiazol-2-yl)-8,9 -dihydro-7H-1,3-dioxolo[4, 5- h] [2, 3] benzodiazepine [00171] A mixture of 0.57 g (1.43 mmol) of the starting material XVIII, 6 rnl of triethyl orthoformate and a catalytic amount of hydrochloric acid was stirred at 80°C for 1 h. After cooling the precipitated crystals were filtered off, washed with ethanol and dried to yield 0.45 g (77%) of the title compound, Mp.: 212-213°G Example 26 (R)-8-Methy 1-5-(4-nitrophenyl)-7-(1,3,4-thiadiazol-2-yl)-8,9-dihydro-7H -1,3-dioxolo[4, 5- h] [2, 3] benzodiazepine [00172] The title compound was obtained from the starting material EX according to the method described in Example 25. Mp.: 144-147°C (ethanol-water). Yield: 88%, [a] D : +428° (c=0.2 ; Choc13) Example 27 <BR> <BR> <BR> <BR> <BR> <BR> (~)-8-Methyl-7-(5-methyl-1,3,4-thiadiazol-2-yl)-5-(4-nitroph enyl)-8,9-dihydro-7H-1, 3-<BR> )-8,9-dih<BR> <BR> <BR> dioxolo [4, 5-h] [2, 3] benzodiazepme [00173] To an ice cooled stirred mixture of 1.0 g (2.50 mmol) of the starting material XVIII, 35 ml of dichloromethane, 0.40 ml (2.75 mmol) of triethylamine and 0.22 ml (2.80 mmol) of acetyl chloride was added. The so obtained solution was left at room temperature for 16 h, then 0.6 g of p-toluenesulfonic acid was added and the mixture was stirred at 40°C for 2 h.

Then the reaction mixture was washed with sodium hydrogen carbonate solution and water until neutrality, dried and concentrated. The crude product was treated with methanol, then recrystallized from ethanol to yield 0.99 g (91%) of the title compound. Mp.: 213-215°C.

Example 28 <BR> <BR> <BR> <BR> <BR> (R)-8-Methyl-7- (5-niethyl-lJ. 4-thmdiazol-2-yl)-5- (4-nitrophenyl)-8, 9-dmydro-7H-1. 3-<BR> )-8,9-dih<BR> <BR> dioxolo[4,5-h][2, 3] benzodiazepine Method A.

[00174] The title compound was obtained from the starting material XIX by carrying out the acylation with acetic anhydride according to the method described In Example 27. The obtained crude product was purified by column chromatography using silica gel (MN Kieselgel 60) as adsorbent and a mixture of n-hexane-ethyl acetate (1: 1) as eluent. After concentration of the fractions containing the title compound, the residue was treated with isopropyl ether to yield 0.95 g of a solid foam (polymorph). Yield: 89%.

Method B.

[00175] To a solution of 4.04 g (10.0 mmol) of the starting material XII, 3 ml of dimethylformamide, 1.40 ml (10.0 mmol) of triethylamine and 0.06 g (0.5 mmol) of 4- dimethylaminopyridine 1.48 g (20.0 mmol) of acetic hydrazide was added. The reaction mixture was stirred at 50°C for 5 h, then diluted with water, the precipitated crystals were filtered off and washed with water. The so obtained 4.5 g of (R)-N'-{8-methyl-5-(4-nitrophenyl)-8, 9-dihydro- 7H-1,3-dioxolo [4,5-h] [2,3] benzodiazepine-7-carbothioyl}-acetic hydrazide according to its 1H- NMR spectrum was a mixture of rotation isomers. (The analyzed sample was purified by column chtomatography using a mixture of n-hexane-ethyl acetate (1: 1) as eluent and it was crystallized with 0.5 mol of ethyl acetate, Mp.: 118°C).

[00176] To a suspension of the above intermediate in 50 ml of ethanol 0.75 ml of concentrated hydrochloric acid was added, and the so obtained solution was refluxed for 2 h.

After concentration and treatment with water 4. 2 g of a crude product was obtained.

Purification by column chromatography using silica gel Kieselgel 60) as adsorbent and a mixture of n-hexane-ethyl acetate as eluent and drying at 60°C in vacuum yielded the title compound with a melting point of 101-102°C. [α]D : +453° (c=0.5 ; CHCl3).

[00177] The compounds of Examples 29-34 were obtained according to the method described in Example 27 using the appropriate acid chlorides.

Example 29 <BR> <BR> <BR> <BR> <BR> <BR> ()-7- yclopropyl-1,3,4-thiadiazol-2-yl)-8-methyl-5-(4-nitrophenyl) -8, 9-dihydro-7H- 1, 3-dioxolo[4,5-h][2, 3] benzodiazepine [00178] Mp.: 142-145'q-yield : 49%.

Example 30 (~)-7-(5-Ethyl-1,3,4-thiadiazol-2-yl)-8-methyl-5-(4-nitrophe nyl)-8,9-dihydro-7H-1,3- dioxolo[4, 5-h] [2, 3] benzodiazepine [00179] Mp.: 163-164°C ; yield: 84%.

Example 31 (R)-7-(5-Ethyl-1,3,4-thiadiazol-2-yl)-8-methyl-5-(4-nitrophe nyl)-8,9-dihydro-7H-1, 3- dioxolo [4, 5-h] [2, 3]benzodiazepine [00180] Mp.: 105°C; yield : 63%. [a] D : +418° (c= 0.5 ; CHCl3).

Example 32 (~)-8-Methyl-5-(4-nitrophenyl)-7-(5-trifluoromethyl-1,3,4-th iadiazol-2-yl)-8,9-dihydro-7H- 1, 3-dioxolo [4, 5-h] [2. 3] benzodiazepine [00181] Mp.: 184-185°C; yield: 67%.

Example 33 (~)-8-Methyl-5-(4-nitrophenyl)-7-(5-phenyl-1,3,4-thiadiazol- 2-yl)-8,9-dihydro-7H-1,3- dioxolo[4,5-h][2, 3] benzodiazepine [00182] Mp.: 210-212'C-, yield : 56%.

Example 34 ()-7- (5-Chloromethyl-1,3,4-thiadiazol-2-yl)-8-methyl-5-(4-nitroph enyl)-8,9-dihydro-7H- 1, 3-dioxolo[4,5-h][2,3]benzodiazepine [00183] Mp.: 210-211°C ; yield : 64%.

Example 35 (~)-7-(5-Cyclopropylaminomethyl-1,3,4-thiadiazol-2-yl)-8-met hyl-5-(4-nitrophenyl)-8,9- dihydro-7H-1, 3-dioxolo [4, 5-h] [2, 3] benzodiazepine [00184] A mixture of 5 rnl of dimethylformaxnide, 0.44 g (0.96 mmol) of ()-7- (5- chloromethyl-1, 3, 4-thiadiazol-2-yl)-8-methyl-5- (4-nitrophenyl)-8, 9-dihydro-7H-1, 3-dioxolo [00185] [4,5-h] [2, 3] benzodiazepine (Example 34) and 0.37 ml (5.31 mmol) of cyclopropylamine was stirred at 70-80°C for 1 h. Then the reaction mixture was poured into 20% sodium chloride solution and, the precipitated crude product was extracted into ethyl acetate. The solution was washed with water, dried and after evaporation yielded 0.39 g (85%) of the title compound, as solid foam.

Example 36 ()-8-Chloro-4-methyl-l- (4-nitrophenyl)-31, 3, 4-thiadiazol-2-yl)-4, 5-dihydro-3H- [2, 3]benzodiazepine The title compound was obtained from the starting material XX according to the method described in Example 25. Mp.: 188°C; yield : 86% Example 37 (~)-8-Chloro-4-methyl-3-(5-methyl-1,3,4-thiadiazol-2-yl)-1-( 4-nitrophenyl)-4,5-dihydro- 3H- [2, 3] benzodiazepine The title compound was obtained from the starting material XX according to the method described in Example 27. Mp.: 162-164°C; yield : 52%.

Example 38 <BR> <BR> <BR> <BR> <BR> ()-8-Meth,yl-5- 3-methyl-4-nitrophenyl)-7- (5-methyl-1, 3, 4-thiadiazol-2-yl)-8, 9-dihydro-<BR> <BR> <BR> 7H-1, 3-dioxolo [4, 5-h] [2, 3] benzodiazepine [00188] The title compound was obtained from the starting material XNI according to the process described in method A of Example 28.

[00189] Mp.: 228-240'C, yield : 74%.

Example 39 (~)-8-Methyl-5-(4-methyl-3-nitrophenyl)-7-(5-methyl-1,3,4-th iadiazol-5-yl)-8,9-dihydro- 7H-1, 3-dioxolo [4, 5-h] [2, 3] benzodiazepine [00190] The title compound was obtained from the starting material XV according to the process described in method B of Example 28.

[00191] Mp.: 220°C (ethanol) ; yield : 57%.

Example 40 (~)-8-Methyl-7-(5-methyl-1,3,4-thiadiazol-2-yl)-5-(3-nitroph enyl)-8,9-dihydro-7H-1, 3- dioxolo 4, 5-h] [2, 3] benzodiazepine [00192] The title compound was obtained from the starting material XIII according to the process described in method B of Example 28.

[00193] Mp. : 118-119°C ; yield: 67%.

Example 41 (~)-7-Bromo-4-methyl-3-(5-methyl-1,3,4-thiadiazol-2-yl)-8-me thoxy-1-(4-nitrophenyl)4, 5- dihydro-3H-[2, 3] benzodiazepine [00194] The title compound was obtained from the starting material according to the process described in method A of Example 28.

[00195] Mp.: 229-233'q-yield : 76%.

Example 42 (~)-8-Methyl-7-(5-methyl-6H-1,3,4-thiadiazin-2-yl)-5-(4-nitr ophenyl)-8,9-dihydro-7H-1,3- dioxolo[4,5-h][2,3]benzodiazepine [00196] A mixture of 1.00 g (2.50 mmol) of the starting material XVIII, 20 ml of dimethylformamide and 0.57 g (6.16 mmol) of chloroacetone was stirred at room temperature for 2 h. After dilution with water the precipitated crystals were filtered off and purified by refluxing in ethyl acetate to yield 0.73 g (67%) of the title compound ; Mp.: 203-204°C.

Example 43 <BR> <BR> <BR> <BR> <BR> (~)-7-(5,6-Dihydro-5-oxo-4H-1,3,4-thiadiazin-2-yl)-8-methyl- 5-(4-nitrophenyl)-8, 9-<BR> <BR> <BR> dihydro-7H-1, 3-dioxolo [4. 5-h] [2. 3] benzodiazepine [00197] A mixture of 1.00 g (2.50 mmol) of the starting material XVIII, 20 ml of dimethylformamide and 0.94 g (6.14 mmol) of methyl bromoacetate was stirred at 70°C for 1.5 h. After dilution with water the precipitated crystals were filtered off and purified by refluxing in ethyl acetate to yield 0.41 g (37%) of the title compound; Mp.: 294-295°C (dec.).

Example 44 (~)-8-Methyl-5-(4-nitrophenyl)-7-(5-oxo-4,5-dihydro-1,3,4-th iadiazol-2-yl)-8,9-dihydro- 7H-1,3-dioxolo[4,5-h][2,3]benzoidazepine [00198] A mixture of 2.14 g (4.69 mmol) of the starting material : and 122 ml of concentrated hydrochloric acid was stirred at 80°C. A solid material precipitated from the starting solution. The reaction mixture was concentrated to about half of its volume, diluted with 40 rnl of water and made alkaline with sodium hydrogen carbonate solution. The precipitated product was filtered off and washed with water to yield 1.40 g (70%) of the title compound. Mp. : 288°C.

Example 45 <BR> <BR> <BR> <BR> (R)-8-Methyl-5-(4-nitrophenyl)-7-(5-methyl-1, 3, 4-oxadiazol-2-yl)-8,9-dihydro-7H-1, 3-<BR> <BR> <BR> dioxolo[4,5-h][2,3]benzodiazepine [00199] A stiiied mixture of 2.2 g (5.15 mmol) of (R)-N'-(8-methyl-5-(4-nitrophenyl)-8, 9- dihydro-7H-1, 3-dioxolo [4,5-h] [2,3] benzodiazepin-7-carbothioyi)-acetic hydrazide (an intermediate of method B of Example 28), 44 rnl of ethanol and 1.72 g (5.39 mmol) of mercury (II) acetate was refluxed for 2 h. The residue obtained on concentration was dissolved in dichloromethane and filtered through a neutral aluminum oxide column. After washing the column the filtrate was concentrated and the residue was purified by column chromatography using silica gel (MN Kieselgel 60) as adsorbent and a mixture of n-hexane-ethyl acetate (1: 2.5) as eluent to yield 1.07 g (51%) of the title compound. Mp.: 202-204°C after recrystallization from ethanol. [a] :-249° (c=0. 22; CHCl3).

Example 46 <BR> <BR> <BR> <BR> ()-8-Methyl-7- (2-methyl-3-oxo-2, 3-dihydro-1, 2. 4-thiadiazol-5-yl)-5- (4-mtrophenyl)-8, 9- dihydro-7H-1, 3-dioxolo [4 5-h] [2, 3] benzodiazepine [00200] To an ice cooled stirred solution of 0.44 g (1. 0 mmol) of the starting material XXVII in 8 ml of chloroform a solution of 0.19 g (1.2 mmol) of bromine in 3 rnl of chloroform was added. After 0.5 h the reaction mixture was diluted with 15 ml of chloroform and washed with sodium hydrogen carbonate solution and water. The residue obtained on concentration was stirred with methanol and filtered to yield 0.36 g (82%) of the title compound. Mp.: 296°C after recrystallization from ethyl acetate.

[00201] The compounds of Example 47 and 48 were obtained analogously from the starting materials XXVIII and XXIX, respectively.

Example 47 (~)-7-(2-Cyclopropyl-3-oxo-2,3-dihydro-1,2,4-thiadiazol-5-yl )-8-methyl-5-(4-nitrophenyl)- 8, 9-dihydro-7H-1, 3-dioxolo [4, 5-h] [2 3] benzodiazepine [00202] Mp.: 246-247°C (ethyl acetate), yield : 64%.

Example 48 (~)-7-(2-Ethyl-3-oxo-2,3-dihydro-1,2,4-thiadiazol-5-yl)-8-me thyl-5-(4-nitrophenyl)-8,9- dihydro-7H-1,3-dioxolo[4,5-h][2, 3] benzodiazepine [00203] Mp.: 250-256°C, yield: 60%.

Example 49 <BR> <BR> <BR> <BR> <BR> (~)-7-(4-Carboxythiazol-2-yl)-8-methyl-5-(4-nitrophenyl)-8,9 -dihydro-7H-1,3-dioxolo[4,5-<BR> <BR> <BR> h] [2 3] benzodiazepine [00204] A mixture of 9 ml of ethanol, 0.85 g (1.89 mmol) of ()-7- (4-ethoxycarbonyl- thiazol-2-yl)-8-methyl-5- (4-nitrophenyl)-8, 9-dihydro-7H-1, 3-dioxolo [4, 5-h] [2, 3] benzodiazepine (Example 8) and 7 rnl of IN sodium hydroxide solution was stirred at 90°C. After cooling, it was acidified with acetic acid, diluted with water and the precipitated crystals were filtered off, washed with water and dried to yield 0.78 g (98%) of the title compound; Mp.: >260°C.

Example 50 <BR> <BR> <BR> <BR> <BR> ()-8-Methyl-5- (4-nitrophenyl)-7- (5-tEtmzolyl)-8. 9-dihydro-7H-1. 3-dioxolo [4, 5-<BR> <BR> <BR> h] [2, 3] benzodiazepine [00205] A mixture of 0.60 g (1.70 mmol) of the starting material XNII, 3 ml of dimethylformamide, 0.12 g (1.87 mmol) of sodium azide and 0.10 g (1.87 mmol) of ammonium chloride was stirred at 140°C for 30 min. The cooled reaction mixture was diluted with water and the precipitated crystals were filtered off. The so obtained product was purified by column chromatographyusing silica gel (MNKieselgel 60) as adsorbent and a mixture of chloroform- methanol (99: 1) as eluent to yield 0.68 g (54%) of the title compound; Mp.: 263-264°C.

Example 51 [00206] (~)-8-Methyl-5-(4-nitrophenyl)-7-(1,2,4-oxadiazol-3-yl)-8,9- dihydro-7H-1,3- dioxolo[4,5-h][2,3]benzodiazepine [00207] A mixture of 1.50 g (3.91 mmol) of the starting material XXIV and 15 ml of triethyl orthoformate in the presence of 0.05 ml of 36% hydrochloric acid was stirred at 110°C for 30 min, then concentrated in vacuum. The residue was stirred with water, the precipitated crystals were filtered off, washed with water and recrystallized from 2-methoxyethanol to yield 1.15 g (75%) of the title compound; Mp.: 190-196°C.

Example 52 ()-8-Methyl-7- (5-methyl-l, 2, 4-oxadmzol-3-yl')-5- (4-mtrophenyl)-8, 9-dihydro-7H-l, 3- dioxolo[4,5-h][2,3]benzodiazepine [00208] A mixture of 3.0 g (7.82 mmol) of the starting material XXIV and 15 ml of acetic anhydride was stirred at 110°C for 1 h, then after cooling it was diluted with water and extracted with dichloromethane. The organic layer was concentrated and the residue was purified by column chromatography using silica gel (MN Kieselgel 60) as adsorbent and a mixture of n- hexane-ethyl acetate (2: 1) as eluent to yield 1.58 g (50%) of the title compound; Mp.: 191- 200°C.

Example 53 (~)-8-Methyl-7-(2-methylthiazol-4-yl)-5-(4-nitrophenyl)-8,9- dihydro-7H-1,3-dioxolo[4,5- hl_j2, 3] benzodiazepine Step A (~)-7-Bromoaoetyl-8-methyl-5-(4-nitrophenyl)-8,9-dihydro-7H- 1,3-dioxolo[4,5-b][2,3]benzodiazepine [00209] A mixture of 4.80 g (14.7 mmol) of the starting material I, 24 ml of dimethylformamide, 2.16 g (15.5 mmol) of bromoacetic acid and 4.56 g (22 mmol) of dicyclohexylcarbodiimide was stirred for 20 h. The reaction mixture was filtered and the filtrate was concentrated. The residue was taken up in ethyl acetate, washed with water, concentrated and recrystallized from ethanol to yield 4.83 g (73%) of the title compound; Mp.: 183-186°C.

Step B [00210] The product obtained in Step A was dissolved in 45 ml of dimethylformamide and after adding 4.96 g (65 mmol) of thioacetamide it was stirred at 80°C for 1 h, then cooled and poured into water. The precipitated crude product was filtered off, washed with water and purified by column chromatography using silica gel (M Kieselgel 60) as adsorbent and a mixture ofhexane-ethyl acetate (9: 1) as eluent to yield 1.67 g (37%) of the title compound ; Mp.: 178-190°C.

Example 54 (~)-8-Methyl-5-(4-nitrophenyl)-7-(2-pyrimidinyl)-8,9-dihydro -7H-1,3-dioxolo[4, 5- h] [2, 3] benzodiazepine Step A 1-{6-[(4-Nitrophenyl)-(pyrimidin-2-yl-hydrazono)-methyl]-ben zo-1,3-dioxol-5-yl}-propan-2-ol [00211] A stirred mixture of 3.29 g (9.99 mmol) of ()-7-methyl-5- (4-nitrophenyl)-7, 8- dihydro-5H-[1, 3] dioxolo [4,5-g] isochroman-5-ol, 40 ml of ethyl acetate and 1.0 ml (1.15 mmol) of perchloric acid-was refluxed for 1 h. After cooling the precipitated ()-7-methyl-5- (4- nitrophenyl)-7, 8-dihydro- [1, 3] dioxolo [4, 5-g] isochromen-6-ylium perchlorate was filtered off, and it was stirred at reflux temperature with 1.6 g (14.55 mmol) of 2-hydrazinopynmidine in 50 ml of isopropanol for 2 h, then concentrated. The residue was dissolved in dichloromethane and washed several times with water. After drying and evaporation the crude product was purified by column chromatography using silica gel (EKieselgel 60) as adsorbent and a mixture of toluene-ethyl acetate (0.1 : 4) as eluent to yield 2.71 g (64%) of the title compound ; Mp.: 125- 127°G Step B 1-{6-[(4-Nitrophenyl)-(pyrimidin-2-yl-hydrazono)-methyl]-ben zo-1,3-dioxol-5-yl}-propan-2-ol mesylate [00212] 2. 35 g (5. 58 mmol) of the compound prepared in Step A was dissolved in 50 ml of dry dichloromethane. The solution was cooled to 0 °C and after addition of 2.1 ml (15.07 mmol) of triethylamine 0.87 rnl (11.22 mmol) of methanesulfonyl chloride was added over a period of 20 min, then the mixture was stirred at room temperature for 3 h. After washing with water it was dried and concentrated to yield 2.69 g (54%) of the title compound as an intermediate; Mp. : 122-124°C.

Step C [00213] A mixture of 3.13 g (6.27 mmol) of the compound obtained in Step B, 60 ml of a 1: 1 mixture of dichloromethane-methanol and 0.52 ml (6. 90 mmol) of 50% sodium hydroxide solution was stirred at room for 1.5 h. After filtration the reaction mixture was concentrated, the residue was treated with water and recrystallized from three fold dimethylformamide containing 10 % water to yield 1.96 g (77%) of the title compound; Mp.: 261-263°C.

Example 55 (~)-7-(3-Chloropyridazin-6-yl)-8-methyl-5-(4-nitrophenyl)-8, 9-dihydro-7H-1,3- dioxolo [4, 5-h] [2, 3] benzodiazepine Step A 1-{6-[(6-Chloropyrdazin-3-yl)-hydrazono-(4-nitrophenyl)-meth yl]-(benzo-1,3-dioxol-5-yl)}-propan-2-ol [00214] A stirred mixture of 2.00 g (6.07 mmol) of ()-7-methyl-5- (4-nitrophenyl)-7, 8- dihydro-5H-[1, 3] dioxolo [4,5-g] isochroman-5-ol, 32 rnl of isopropanol, 0.3 ml of hydrochloric acid and 1.04 g (7. 28 mmol) of 4-hydrazino-6-chloropyndazine was refluxed for 3 h. After diluting with water, the precipitated crystals were filtered off, dried and recrystallized first from ethyl acetate, then from dimethylformamide containing 10% water to yield 1.53 g (55%) of the title compound; Mp.: 135-137°C.

Step B [00215] A mixture of 0.3 g (0.66 mmol) of the compound prepared in Step A, 10 ml of dimethylformamide and 0.34 g (1.30 mmol) of triphenylphosphine was stirred at room temperature for 5 min, then 0.20 ml (1.27 mmol) of diethyl azodicarboxylate was added and stirring was continued for 24 h. After dilution with sodium chloride solution the precipitated product was filtered off, dried and purified by column chromatography using silica gel (MN Kieselgel 60) as adsorbent and a mixture of chloroform-methanol (99: 1) as eluent. The residue obtained on concentration was crystallized byrefluxing in ethanol to yield 0.12 g (42%) of the title compound; Mp.: 254-255°C.

Example 56 (~)-8-Methyl-5-(4-nitrophenyl)-7-(1H(2H)-1,2,4-triazol-3-yl) -8,9-dihydro-7H-1,3- dioxolo [4, 5-h] [2, 3] benzodiazepine Step A (~)-8-Methyl-5-(4-nitrophenyl)-8,9-dihydro-7H-1,3-dioxolo[4, 5-b][2,3]benzodiazepine-7-S-methyl- thiocarboximidate [00216] The title compound was obtained from the starting material I in dimethylformamide with methyl iodide in the presence of potassium carbonate at room temperature. Mp.: 191-192°C, yield : 94%.

Step B [00217] A mixture of 3.0 g (7.53 mmol) of the compound obtained in Step A, 110 ml of 2-methoxyethanol and 4.50 g (74.93 mmol) of formic hydrazide was stirred at 110°Cin the presence of catalytic amount of p-toluenesulfonic acid for 16 h. The residue obtained on concentration was treated with 10 % sodium carbonate solution, the obtained crude product was filtered, dried and purified by column chromatography using silica gel (MN Kieselgel 60) as adsorbent and a mixture of hexane-ethyl acetate (1: 2) as eluent to yield 1.86 g (63%) of the title compound; Mp.: 154-156°C.

Example 57 <BR> <BR> <BR> <BR> <BR> 8-Methyl-7-(5-methyl-2 (1) H-1. 2, 4-triazol-3-yl)-5- (4-nitrophenyl)-8,9-dihydro-7H-1,3-<BR> <BR> <BR> <BR> dioxolo [4, 5- h] [2, 3] benzodiazepine [00218] A mixture of 15 ml of 2-methoxyethanol, 0.41 g (1.03 mmol) of ()-8-methyl-5- (4-nitrophenyl)-8, 9-dihydro-7H-1, 3-dioxolo [4, 5-h] [2,3] benzodiazepine-7-S-methyl- thiocarboximidate (Step A of Example 56) and 0.35 g (4.68 mmol) of acetic hydrazide was stirred at 110°C in the presence of catalytic amount of p-toluenesulfonic acid for 16 h. The residue obtained on concentration was treated with 10% sodium carbonate solution, the obtained crude product was filtered, dried and purified by column chromatography using silica gel (MN Kieselgel 60) as adsorbent and a mixture of hexane-ethyl acetate (1: 2) as eluent to yield 0.32 g (78%) of the title compound; Mp.: 144-147°C (solid foam).

Example 58 (~)-7-(1,5-Dimethyl-1H-1, 2, 4-triazol-3-yl)-8-methyl-5-(4-nitrophenyl)-8,9-dihydro-7H-1, 3- dioxolo [4, 5-h] [2, benzodiazepme (isomeri) and (+)-7-(2, 5-dimeliyl-2H-1, 2, 4-triazol-3- yl)-8-methyl-5-(4-nitrophenyl)-8,9-dihydro-7H-1,3-dioxolo[4, 5-h][2, 3] benzodiazepine isomer II [00219] A mixture of 0.57 g (5.08 mmol) of potassium tert-butoxide, 2.05 g (5.04 mmol) of (~)-8-methyl-7-(5-methyl-2(1)H-1, 2, 4-triazol-3-yl)-5- (4-nitrophenyl)-8, 9-dihydro-7H-1, 3- dioxolo [4,5-h] [2,3] benzodiazepine (Example 57), 40 ml of tetrahydrofuran and 0.32 ml (5.14 mmol) of methyl iodide was stirred at room temperature for 16 h. then the reaction mixture was diluted with water, extracted with ethyl acetate, the organic layer was dried and concentrated.

The two products formed in the reaction were separated by column chromatography using silica gel (MN Kieselgel 60) as adsorbent and ethyl acetate as eluent. Isomer II, having RF : 0.55 was first obtained, which was refluxed in ethanol to yield 0.30 g (14 %), Mp.: 185-187 °C. Then isomer7 was collected, having RF : 0.26, which after refluxing in ethanol weighed 0.67 g (32%), Mp.: 193-195°G Example 59 (~)-8-Methyl-7-(1-methyl-1H-1,2,4-triazol-3-yl)-5-(4-nitroph enyl)-8,9-dihydro-7H-1,3- dioxolor4, 5-hlr2, 31benzodiazepine (, isomer 1.) and ()-8-methyl-7- (2-methyl-2H-1, 2,4- riazol-3-yl)-4-nitrophenyl)-8, 9-dihydro-7H-1, 3-dioxolo[4,5-h] [2, 3] benzodiazepine<BR> 1)-8,9-dih<BR> <BR> (isomer II) [00220] A mixture of 0.41 g (3.65 mmol) of potassium tert-butoxide, 1.4 g (3.57 mmol) of ()-8-methyl-5- (4-nitrophenyl)-7- (1H (2E3)-1, 2, 4-triazol-3-yl)-8, 9-dihydro-7H-1, 3-dioxolo [4,5- h] [2,3] benzodiazepine (Example 56), 35 ml of tetrahydrofuran and 0.23 ml (3.69 mmol) of methyl iodide was stirred at room temperature for 16 h. After dilution with water the reaction mixture was extracted with ethyl acetate, the organic layer was dried and concentrated. The two products formed in the reaction were separated by column chromatography using silica gel (MN Kieselgel 60) as adsorbent and ethyl acetate as eluent. Isomer I, having RF : 0.22, weighed 0.37 g, yield: 26%, Mp.: 115-117°c. Isomer II, having RF : 0.63, was 0.35 g, yield: 24 %, Mp.: 92-94°C.

Examples 60-119 General procedrmes for reduction of the nitro groups of the compounds obtained in the aboue examples Method A [00221] 2.0 mmol of nitro compound was dissolved in a mixture of methanol- dichloromethane and after adding 6-10 mmol of 85-98% hydrazine hydrate and 0.1-2 g RaNt catalyst the mixture was stirred at 20-40°C for 1-5 h. After filtration of the catalyst the filtrate was concentrated, the residue was treated with water and the product was filtered off.

Method B [00222] 5.5 g of RaNi catalyst was prehydrogenated in 250 rnl of a 2: 1 mixture of methanol-dichloromethane, then 20.0 mmol of nitro compound was added in 250 ml of the above solvent mixture and the so obtained mixture was hydrogenated at atmospheric pressure.

After filtration of the catalyst the filtrate was concentrated, the residue was treated with water, the product was filtered, washed and dried.

Method C [00223] A stirred mixture of 1.82 mmol of nitro compound, 30 ml of ethanol and 2.46 g (10.91 mmol) of tin (II) chloride dihydrate was refluxed for 3 h. The reaction mixture was concentrated, then aqueous sodium hydrogen carbonate and ethyl acetate were added to the residue. After separation the water phase was extracted with ethyl acetate, the combined organic layers were washed with sodium chloride solution, dried and concentrated. If necessarythe residue was purified either by column chromatography or by recrystallization.

Method D [00224] 3.4 mmol of nitro compound was dissolved in 35 ml of a mixture of methanol- dichloromethane (1: 1), 0.4 g of a 10% palladium on activated carbon catalyst and 0.47 g of potassium carbonate were added and the so obtained mixture was hydrogenated in the presence of 1 ml of water. After completion of the reaction the catalyst was filtered off, the filtrate was concentrated, the residue was treated with water and filtered.

Method E [00225] 4.0 mmol of nitro compound was dissolved in 48 ml of methanol containing 5% water, then after addition of 0.20 g of the catalyst 10% palladium on activated carbon 3.5 equivalent of a concentrated aqueous solution of potassium formate was added dropwise at room temperature and the mixture was stirred at the above temperature. After completion of the reaction the catalyst was filtered off, the filtrate was concentrated, the residue is treated with water and filtered.

Table 10.

2,3-Benzodiazepines containing ammophenyl group (The 1H NMR spectra were recorded at 250 MHz unless stated otherwise) 0 Number of., P- (° Yield (%) Example rec stall. [a] D recrystall. 60 ()-5- (4-Aminophenyl)-8-methyl-7- (2-thiazolyl)-8, 9- 187-190 78 dihydro-7H-1, 3-dioxolo [4, 5-h] [2, 3] benzodiazepine Method 1HNMR (CDC13) 6 1. 32 (3H, d, 6. 5 Hz), 2. 78 (1H, dd, 14. 0 Hz, 9. 7 Hz), 2. 97 (1H, dd, 14. 0 A Hz, 4. 9 Hz), 3. 80 (2H, br), 5. 26 (1X m), 5. 98 (2H, m), 6. 65 (1H, s), 6. 67 (1H, d, 4. 0 Hz), 6. 73 (2H, dm), 6. 80 (1H, s), 7. 37 (1H, d, 4. 0 Hz), 7. 55 (2H, dm) MS : EI (70eV) : M+ : 378, nit : 363, 279, 278, 253, 252 61 (R)-5- (4-Aminophenyl)-8-methyi-7- (2-thiazolyl)-8, 9- 125-130 84 dihydro-7H-1, 3-dioxolo [4, 5-h] [2, 3] benzodiazepine-578° c=1, CHCl3 Method 1HNMR (CDC13) 6 1. 29 (3X d, 6. 5 Hz), 2. 77 (1H, dd, 14. 0 Hz, 9. 7 Hz), 3. 00 (1H, dd, 14. 0 A Hz, 4. 9 Hz), 3. 92 (2H, br), 5. 23 (1H, m), 5. 98 (2H, m), 6. 62 (1X d, 4. 0 Hz), 6. 65 (1H, s), 6. 72 (2H, dm), 6. 80 lH, s, 7. 32 lH, d, 4. 0 Hz), 7. 55 (2X dn l) 62 (S)-5- (4-Aminophenyl)-8-methyl-7- (2-thiazolyl)-8, 9- 124-128 94 dihydro-7H-1, 3-dioxolo [4, 5-h] [2, 3] benzodiazepine +546° c=0. 34, CHC3 Method 1H NMR. (DMSO-d6) 8 1. 15 (3H, d, 6. 5 Hz), 2. 60 (1H, dd, 13. 6 Hz, 10. 5 Hz), 2. 94 (lij dd, A 13. 6 Hz, 4. 8 Hz), 4. 99 (1H, m), 5. 72 (2H, br), 6. 03 (2H, m), 6. 60 (2H, dm), 6. 62 (1H, s), 6. 81 (1H, d, 4. 0 Hz), 7. 04 (1H, s), 7. 27 (1H, d, 4. 0 Hz), 7. 55 (2H, dm) MS : EI (70eV) : M+ : 378, milz : 377, 363, 279, 278, 253, 252 Cl : [M+H] + : 379, [M] : 378, m/z : 363 63 ()-5- (4pinophenyl)-8-methyl-7- (4-methyl-thiazol-2-190-191 65 yl)-8, 9-dihydro-7H-1, 3-dioxolo- (EtOH [4, 5-h] [2, 3] benzodiazepine MethodiHNMR. (CDQ :)) 8 1. 30 (3H, d, 6. 5 Hz), 2. 29 (3H, s), 2. 77 (IK dd, 14. 0 Hz, 10. 0 Hz), 2. 92 (1H, dd, 14. 0 Hz, 5. 1 Hz), 3. 94 (2H, br), 5. 27 (1H, m), 5. 97 (2H, m), 6. 20 (IK s), 6. 53 (2X dm), 6. 70 (1X s), 6. 88 (lI-L s), 7. 53 (2H, dm) 64 ()-5- (4-Aminophenyl)-8-methyl-7- (5-methyl-thiazol-2-165-167 47 yl)-8, 9-dihydro-7H-1, 3-dioxolo- [4, 5-h] [2, 3] benzodiazepine Method 1H NE (DMSO-d) 6 1. 17 (3H, d, 6. 5 Hz), 2. 25 (3H, s), 2. 60 (1H, dd, 13. 9 Hz, 10. 3 Hz), A 2. 94 (IK dd, 13. 9 Hz, 5. 1 Hz), 4. 95 (lI- m), 5. 70 (2H, br), 6. 05 (2H, dm), 6. 57 (1H, s), 6. 62 2H, dm), 6. 93 (1H, s), 7. 04 lH, s), 7. 36 2H, dm) 65 ()-5- (4-Aminophenyl)-8-methyl-7- (4, 5-dimethyl-240-242 83 thiazol-2-yl)-8, 9-dihydro-7H-1, 3-dioxolo- (EtOH) [4, 5-h] [2, 3] benzodiazepine Method 1HNE/DMSOd6) õ 1. 16 (3X d, 6. 5 Hz), 2. 06 (3H, s), 2. 13 (3H, s), 2. 62 (1H, dd, 14. 0 Hz, A 10. 0 Hz), 2. 92 (1H, dd, 14. 0 Hz, 5. 0 Hz), 4. 97 (IK m), 5. 70 (2X br), 6. 04 (2riz dm), 6. 60 (IK s), 6. 62 (2X dm), 7. 02 (1X s), 7. 34 (2H, dm) 66 ()-5- (4-Aminophenyl)-7- (4-phenyi-thiazol-2-yi)-8-221-223 89 methyl-8, 9-dihydro-7H-1, 3-dioxolo- (EtOH) [4, 5-h] [2, 3] benzodiazepine O Number of MP. (OC) Yield Name Solvent of Example recrystall. [ Method lH NE (CDCI3) õ 1. 29 (3H, d, 6. 5 Hz), 2. 80 (lu dd, 14. 0 Hz, 9. 4 Hz), 3. 00 (1H, dd, 14. 0 Dz, 4. 8 Hz), 3. 93 (2H, br), 5. 40 (1H, m), 5. 98 (2X m), 6. 62 (1H, s), 6. 70 (2X dm), 6. 78 (li-L s, 7. 29 lH, t, 7. 39 (lut), 7. 50 (lys), 7. 57 (2X dm), 7. 86 2H, d 67 ()-5- (4-Aminophenyl)-7- (4-ethoxycarbonyl-thiazol-2- 251-252 83 yl)-8-methyl-8, 9-dihydro-7H-1, 3-dioxolo-gtOH) [4, 5-h] [2, 3] benzodiazepine Method 1H NMR (CDCI3) 6 1. 29 (3H, d, 6. 5 Hz), 1. 38 (3X t), 2. 76 (1H, dd, 14. 0 Hz, 10. 0 Hz), 2. 92 (1H, dd, 14. 0 Hz, 5. 0 Hz), 3. 98 (2X br), 4. 33 (2riz q), 5. 40 (IK m), 6. 00 (2riz m), 6. 68 (1H, s) ; 6. 69 2H, dm), 6. 82 (lX s), 6. 86 (H, s), 7. 51 2H, dm) 68 (+)-5- (4-Aminophenyl)-7- (4, 5-dihydro-thiazol-2-yl)-8- 145-150 84 methyl-8, 9-dihydro-7H-1, 3-dioxolo- (EtOI [4, 5-h] [2, 3] benzodiazepine MethodiHNMR (CDQa) 8 1. 21 (3H, d, 6. 5 Hz), 2. 70 (1H, dd, 14. 0 Hz, 10. 0 Hz), 2. 96 (1H, dd, 14. 0 A Hz, 5. 0 Hz), 3. 20 (IK m), 3. 70 (1H, m), 3. 90 (2H, br), 4. 17 (2H, m), 5. 09 (IK m), 5. 98 (2H, dm), 6. 60 (1H, s), 6. 66 (2H, dm), 6. 73 (1H, s), 7. 47 (2H, dm) MS : EI (70eV) : W1+ : 380, m/z : 365, 339, 279, 264, 253, 252 (4-5- (4-Aminophenyl)-7- (4, 5-dihydro-thiazol-2-yl)-8- 148-150 82 methyl-8, 9-dihydro-7H-1, 3-dioxolo-gtOH)-239° (c=0. 5, CHCl3) [4, 5-h] [2, 3] benzodiazepine, Method 1H NMR (DMSO-d6) 8 1. 16 (3H, d, 6. 5 Hz), 2. 60 (1X dd, 14. 0 Hz, 10. 0 Hz), 2. 90 (lX dd, A 14. 0 Hz, 4. 0 Hz), 3. 25 (2riz m), 4. 00 (2H, m), 4. 82 (1X m), 5. 73 (2H, br), 6. 07 (2riz dm), 6. 64 (s), 6. 64 (2H, dm), 7. 02 (1X s), 7. 30 (2H, dm) MS : EI (70eV) : M+ : 380, m/z : 365, 339, 279, 278, 264, 253, 252 CI : [M+H] + : 381, M+ : 380, niz : 279 70 (S)-5- (4-Aminophenyl)-7- (4, 5-dihydro-thiazol-2-yi)-8- 150-152 92 methyl-8, 9-dihydro-7H-l3-dioxolo-+175° {c=O 51, CHC134 [4, 5-h] [2, 3] benzodiazepine Method MS : EI (70eV) : [M1+ : 380, nit : 365, 339, 279, 278, 264, 253, 252 CI : [M+q+ : 381, M+ : 380, mlz : 279 71 (+)-5- (4-Arninophenyl)-7- (4, 5-dihydro-4-oxo-thiazol-2- 218-220 85 yl)-8-methyl-8, 9-dihydra7H-1, 3-dioxolo- (EtOE3) [4, 5-h] [2, 3] benzodiazepine SIethod 1H NMR (DMSOd6) õ 1. 29 (3X d, 6. 5 Hz), 2. 61 (1H3 dd, 13. 0 Hz, 12. 0 Hz), 2. 96 (1H3 dd, A 13. 0 Hz, 5. 0 Hz), 3. 72 (2riz m), 5. 08 (1H, m), 6. 01 (2H, br), 6. 06 (2H, dm), 6. 60 (2H, dm), 6. 62 (1H, s), 7. 10 (1H, s), 7. 40 (H, dm) 72 ()-5- (4-Aminophenyl)-7- (4, 5-dihydro-5-methyl-4-oxo- 200-204 63 thiazol-2-yl)-8-methyl-8, 9-dihydro-7H-1, 3-dioxolo- (EtOH) [4, 5-h] [2, 3] benzodiazepine Method tH NMR) MSO-d3) õ 1. 32 (d) and 1. 45 (d, overlapping, diastereomers), 2. 60 (1H, dd, 13. 0 A Hz, 12. 0 Hz), 2. 94 (1H, dd, 13. 0 Hz, 5. 0 Hz), 3. 96 and 4. 05 (lX q), 5. 08 (1H, m), 6. 0 (2H, br), 6. 07 (2H, dm), 6. 60 (2riz, dm), 6. 62 (lu s), 7. 08 (lI-L s), 7. 40 (2riz dm) MS : EI (70eV) : M+ : 408, n<Jæ 393, 279, 265, 253, 252 O Number of MP. (OQ Yield (%) Example rec tall. [a] D rys 73 (+)-5- (4-Aminophenyl)-7-(5, 6-dihydro-4-oxo-4H-1, 3-226-228 90 thiazin-2-yl)-8-methyl-8, 9-dihydro-7H-1, 3-dioxolo- (EtOH) [4, 5-h] [2, 3] benzodiazepine Method 1HNMR (DMSO-d6) 8 1. 25 (3H d, 6. 5 Hz), 2. 35 (2H, m), 2. 57 (lX dd, 13. 0 Hz, 12. 0 Hz), A 2. 88 (lX dd, 13. 0 Hz, 4. 0 Hz), 3. 05 (2H, m), 5. 21 (1H, m), 5. 97 (2H, br), 6. 09 (2H, dm), 6. 60 (1R s), 6. 62 (2X dm), 7. 04 (1X s), 7. 42 (2riz dm) MS : EI (70eV) : M+ : 408, m/z : 295, 279, 253, 252 74 5- (4-Aminophen Zl)-7-(2-thiazolyl)-8, 9-dihydro-7H-1, 3-200-204 52 dioxolo [4, 5-h] [2, 3] benzodiazepine MethodiHNMR (DMSO-dt) 8 2. 88 (2riz t), 4. 21 (2H, t), 5. 70 (2H, s), 6. 08 (2H, s), 6. 60 (lu s), 6. 62 A (2H, dm), 6. 89 (1H, d, 4. 0 Hz), 7. 08 (1H, s), 7. 28 (1H, d, 4. 0 Hz), 7. 37 (2H, dm) 75 ()-5- (4-Amino-3-methylphenyl)-8-methyl-7- (2- 225-227 78 thiazolyl)-8, 9-dihydro-7H-1, 3-dioxolo- [4, 5-h] [2, 3] benzodiazepine Method MS : EIpOeV) : M+ : 392, naZz : 377, 293, 266 B Cl : : [M+H] + : 393, M+ : 392, mlz : 266 76 ()-I- (4-Aminophenyi)-4-methyl-8-methoxy-3- (2- 105-107 57 thiazolyl)-4, 5-dihydro-3H-[2, 3] benzodiazepine Method MS : EI (70eV) : N1+ : 364, m/z : 349, 265, 223 d : [M+H] + : 365, [W+ : 364 77 ()-1- (4_ophenyl)-8-chloro-4-methyl-3- (2- 104-107 72 thiazolyl)-4, 5-dihydro-3H- [2, 3] benzodiazepine l Method lH NMR (CDC13) õ 1. 31 (3X d, 6. 5 Hz), 2. 96 (1if dd, 13. 0 Hz, 10. 0 Hz), 3. 10 (1H, dd, 13. 0 A Hz, 5. 0 Hz), 5. 35 (lu m), 6. 68 (1H, d, 4. 0 Hz), 6. 72 (2riz dm), 7. 21 (IK d, 4. 0 Hz), 7. 25 (1H, d, 1. 0 Hz), 7. 27 (1H, d, 7. 0 Hz), 7. 34 (1H, dd), 7. 53 (2H, dm) 78 ()-1- (4-Aminophenyl)-8-chloro-4-methi-3- (4-methyl-173-17S 90 thiazol-2-yl)-4, 5-dihydro-3H-[2, 3] benzodiazepine MethodiHNMR (CDCh) 8 1. 26 (3H, d, 6. 5 Hz), 2. 27 (3X d, 1. 0 Hz), 2. 81 (1H, dd, 14. 0 Hz, 9. 7 Hz), 3. 02 (1I dd, 14. 0 Hz, 5. 0 Hz), 3. 95 (2H, br), 5. 28 (1H, m), 6. 20 (lu q, 1. 0 Hz), 6. 70 (2H, dm), 7. 17 (1H, d, 2. 2 Hz), 7. 22 (1H, d, 8. 2 Hz), 7. 33 (1H, dd, 8. 2 Hz, 2. 2H), 7. 51 (2H, dm) 79 (_)-1- (4-Aminophenyl)-3- (4, 5-dihydro-thiazol-2-yl)-8^ 213-216 79 chloro-4-methyi-4, 5-dihydro-3H- [2, 3] benzodiazepine (eyed Method IH NMR PIZad6) 8 1. 08 (3H, d, 6. 5 Hz), 2. 68 (1Et dd, 14. 0 Hz, 10. 0 Hz), 3. 06 (IK dd, A 14. 0 Hz, 5. 0 Hz), 3. 20 (2riz m), 4. 02 (2H, m), 5. 68 (2riz s), 4. 92 (1Et m), 6. 60 (2riz dm), 7. 09 (1H, d, 1. 0 Hz), 7. 28 (2H, dm), 7. 41 (H, d, 7. 0 Hz), 7. 48 (1H, dd) 80 ()-l- (4-Aminophenyl)-3- (4, 5-dihydro 4-oxo-thiazol-2-226-228 75 yl)-8-chloro-4-methyl-4, 5-dihydro-3H [2, 3] benzodiazepine --- Number of Name Solvent of Y*leld (%) Example [a] D recrystall. Method íH NMR (DMSOd6) õ 1. 32 (3X d, 6. 5 Hz), 2. 68 (1H, dd, 13. 8 Hz, 12. 0 Hz), 3. 08 (II-L dd, A 13. 8 Hz, 4. 8. Hz), 3. 77 (2H, m), 5. 10 (1H, m), 6. 12 (2X br), 6. 66 (2X dm), 7. 17 (lH, d, 2. 0 Hz), 7. 412H, dm, 7. 52 H, d, 8. 0 Hz), 7. 541H, dd, 8. 0 Hz, 2. oh 81 ()-1- (4-Aminophenyl)-7, 8-dichloro-3- (4-methyl-182-184 48 thiazol-2-yl)-4-methyl-4, 5-dihydro-3H- (EtOI [2, 3] benzodiazepine Method 1H NMR (CDCI3) õ 1. 28 (3H, d, 6. 5 Hz), 2. 30 (3H, s), 2. 80 (1H, dd, 14. 0 Hz, 9. 6 Hz), 3. 02 A (II-L dd, 14. 0 Hz, 4. 9 Hz), 3. 96 (2H, br), 5. 31 (lu m), 6. 22 (lu q, 1. 0 Hz), 6. 69 (2H, dm), 7. 28 lI s), 7. 39 (1X 5), 7. 50 (2H, dm) 82 ()-5- (4-Aminophenyl)-7- (4, 5-dihydro-oxazol-2-yl)-8- 166-167 87 methyl-8, 9-dihydro-7H-1, 3-dioxolo- (EtOH) [4, 5-h] [2, 3] benzodiazepine Method tH NMR (DMSOd6) õ 1. 20 (3H, d, 6. 5 Hz), 2. 31 (1H, dd, 13. 8 Hz, 12. 0 Hz), 2. 78 (lX dd, A 13. 8 Hz, 5. 8 Hz), 3. 61 (2H, m), 4. 18 (2H, m), 4. 51 (1H, m), 5. 66 (2H, br), 6. 03 (2H, dm), 6. 51 (1H, s), 6. 53 (2H, dm), 6. 98 (1H, s), 7. 30 (2H, dm) MS : EI (70eV) : M+ : 364, rdz : 349, 323, 279, 278, 252 CI : [M+H] + : 365, [: K+ : 364 83 ()-5- (4-Aminophenyl)-8-methyl-7-(1, 3, 4-thiadiazol2- 192-194 77 yl)-8, 9-dihydro-7H- 1, 3-dioxolo- (50 % EtOH IO) [4, 5-h] [2, 3] benzodiazepine Method iHNMR (DMSOd6) õ 1. 20 (3H, d, 6. 5 Hz), 2. 62 (lux dd, 13. 9 Hz, 10. 8 Hz), 2. 99 (liez dd, A 13. 9 Hz, 5. 2 Hz), 5. 01 (1H, m), 5. 78 (2riz br), 6. 03 (2H, dm), 6. 58 (1H, s), 6. 60 (21-L dm), 7. 07 lI s), 7. 32 2H, dm,) 84 (R)-5- (4-Aminophenyl)-8-methyl-7- (1, 3, 4-thiadiazol-2- 219-220 67 yl)-8, 9-dihydro-7H-1, 3-dioxolo- (ethyl-490° formate) (c=0. 9, CHCL3) [4, 5-h] [2, 3] benzodiazepine Method 1H NMR (CDC13) õ 1. 33 (3H, d, 6. 5 Hz), 2. 80 (1H, dd, 14. 0 Hz, 9. 9 Hz), 2. 97 (1H, dd, 14. 0 Hz, 5. 0 Hz), 4. 02 (2H, br), 5. 30 (1X m), 5. 98 (2H, dm), 6. 65 (1X s), 6. 68 (2X dm), 6. 80 (1H, s), 7. 51 (2X dm,), 8. 50 (1H, s) 85 ()-5- (4-Aminophenyl)-8-methyl-7- (5-methyl-1, 3, 4- 143-148 89 thiadiazol-2-yl)-8, 9-dihydro-7H-1, 3-dioxolo- [4, 5-h] [2, 3] benzodiazepine Method IH (3) b 1. 32 (3H d, 6. 5 Hz), 2. 56 (3H s), 2. 76 (1H, dd, 14. 0 Hz, 10. 0 Hz), 2. 93 A (1H, dd, 14. 0 Hz, 5. 0 Hz), 4. 00 (2H, br), 5. 19 (1H, m), 5. 98 (2H, dm), 6. 64 (1ER s), 6. 70 (2H, dm), 6. 79 (1X s), 7. 48 (2X dm,) MS : EI (70eV) : M+ : 393, n/z : 378, 279, 278, 253, 252 Cl : [M+EC+ : 394, [}/U+ : 393, rnlz : 252 86 (R)-5- (4-Arninophenyl)-8-methyl-7- (5-methyl-1, 3, 4- 168-170 78 (50 % EtOH-482° thiadiazol-2-yl)-8, 9-dihydro-7H 1, 3-dioxolo- Hz0) (C-0. 5, CICl3) (c=0. 5, CH0. 3) [4, 5-h] [2, 3] benzodiazepine Method 1HNMR (DMSO-d6) õ 1. 23 (3X d, 6. 5 Hz), 2. 50 (3X s), 2. 60 (liez dd, 13. 8 Hz, 9. 6 Hz), 2. 97 B, C : (lX dd, 13. 8 Hz, 4. 9 Hz), 4. 93 (IK m), 5. 78 (2riz br), 6. 03 (2riz dm), 6. 58 (IK s), 6. 60 (2riz dm), 7. 09 (1X S) ? 7. 31 (2X dm) 0 Numberof Mp. (°C) Yield (%) Example [a] D rec stall. 87 ()-5- (4-Aminophenyl)-7- (5-cyclopropyl-1, 3, 4- 145-148 75 (precipit. thiadiazol-2-yl)-8-methyl-8, 9-dihydro-7H-1, 3- wkh water) dioxolo [4, 5-h] [2, 3] benzodiazepine Method 1H NMR (pMSO-d6) õ 0. 88 (2H, m), 1. 05 (2H, m), 1. 22 (3H, d, 6. 5 Hz), 2. 22 (1'I-I, m), 2. 61 A H, dd, 14. 0 Hz, 10. 0 Hz), 2. 99 (1H, dd, 14. 0 Hz, 5. 0 Hz), 4. 97 (1H, m), 5. 78 (2riz br), 6. 05 2H, dm), 6. 60 (1X s), 6. 63 (2H, dm), 7. 06 (lI-L s), 7. 36 (2X dm)' 88 ()-5- (4-Aminophenyl)-7- (5-ethyl-1) 3, 4-thiadiazol-2-yl)- 135-138 67 8-methyl-8, 9-dihydro-7H-1, 3-dioxolo- [4, 5-h] [2, 3] benzodiazepine MethodiHNMR (CDCI) 8 1. 35 (3H, t), 1. 36 (3X d, 6. 5 Hz), 2. 79 (II dd, 14. 0 Hz, 10. 0 Hz), 2. 98 A (2X q), 2. 99 (liez dd, 14. 0 Hz, 5. 0 Hz), 3. 98 (2H, br), 5. 25 (1H, m), 6. 02 (2H, dm), 6. 63 (1H, s), 6. 73 (2X dm), 6. 82 (H, s), 7. 51 (2H, dm,) 89-5- (4-Aminophenyl)-7- (5-ethyl-1, 3, 4-thiadiazol-2-142-144 47 (precipit.-602° yl)-8-methyl-8, 9-dihydro-7H-1, 3-dioxolo- (precipit.-602° with water) (c=0. 5, EtOH) [4, 5-h] [2, 3] benzodiazepine Method MS : EI (70eV) : [w+ : 407, inlz : 392, 279, 278, 253, 252 E CI : [M+H] + : 408, M+ : 407 n 90 ()-5- (4-Arninophen9-8-methyl-7-(5-trifluoromethyl-216-218 33 1, 3, 4-thiadiazol-2-yl)-8, 9-dihydro-7H-1, 3-dioxolo- [4, 5-h] [2, 3] benzodiazepine Method 1HNMR (CDC) s 1. 39 (3H, d, 6. 5 Hz), 2. 80 (1H, dd, 14. 0 Hz, 10. 0 Hz), 2. 93 (lX dd, 14. 0 Dz, 5. 0 Hz), 4. 06 (2H, br), 5. 28 (1H, dm), 6. 00 (2riz dm), 6. 61 (1if s), 6. 69 (2H, dm), 6. 81 (1if s), 7. 48 (2riz dm,) MS : EI (70eV) : §l+ : 447, mlz : 432, 279, 253, 252 Cf : [M+H] + : 448, M+ : 447, m/z : 252 91 ()-5- (¢ophenyl)-7- (5-phenyl-1, 3, 4-thiadiazol-2- 228-230 84 yl)-8-methyl-8, 9-dihydro-7H-1, 3-dioxolo- (50 % EtOH I-3z0) [4, 5-h] [2, 3] benzodiazepine Method 1HNMR (DMSO-d6) õ 1. 28 (3H, d, 6. 5 Hz), 2. 67 (1H, dd, 14. 0 Hz, 10. 0 Hz), 3. 01 (1H, dd, A 14. 0 Hz, 5. 0 Hz), 5. 02 (lu m), 5. 81 (2riz br), 6. 07 (2H, dm), 6. 59 (1X s), 6. 61 (2riz dm), 7. 08 (1H, s), 7. 40 (2riz dm), 7. 45 (3H, m), 7. 81 (2riz d) MS : EI (70eV) : [w+ : 455, mlz : 440, 295, 279, 253, 252 CE : [M+H] + : 456, M+- : 455, m/z : 295 92 ()-5- (4-Aminophenyl) 7- (5-cyclopropylamino-methyl-135-138 35 1, 3, 4-thiadiazol-2-yl)-8-methyl-8, 9-dihydro-7H-1, 3- dioxolo [4, 5-h] [2, 3] benzodiazepine Method 1HNE (GD (: 13) õ 0. 45 (4H, m), 1. 33 (3X d, 6. 5 Hz), 2. 28 (IK m), 2. 75 (1H, dd, 14. 0 Hz, A 9. 9 Hz), 2. 85 (IK dd, 14. 0 Hz, 4. 9 Hz), 4. 0 (2H, br), 4. 10 (2H, s), 5. 26 (1H, m), 6-00 (2Et m), 6. 60 (1H, s), 6. 68 (2H, dm), 6. 80 (1H, s), 7. 49 (2H, dm) 93 ()-1- (4-Aminophenyl)-8-chloro-4-methyl-3- (1, 3, 4- 125-128 79 thiadiazol-2-yi)-4, 5-dihydro-3H-[2, 3] benzodiazepine 0 Number of MP. (OC) Yield (%) < Name Solventot ri Example rec stall. [a] D recrystall. Method IH NMR (DMSOd6) õ 1. 18 (3X d, 6. 5 Hz), 2. 69 (liez dd, 14. 0 Hz, 10. 8 Hz), 3. 14 (1H, dd, 14. 0 Hz, 5. 1 Hz), 5. 05 (lu m), 5. 83 (2H, s), 6. 62 (2X dm), 7. 10 (1if s), 7. 33 (2H, dm), 7. 51 2H, m) 94 ()-l- (4-Aminophenyl)-8-chloro-4-methyl-3- (5-methyl- 131-133 88 1, 3, 4-thiadiazol-2-yl)-4, 5-dihydro-3H- [2, 3] benzodiazepine Method 1H NE (DMSOd6) õ 1. 18 (3X d, 6. 5 Hz), 2. 70 (IK dd, 14. 0 Hz, 10. 3 Hz), 3. 11 (1H, dd, A 14. 0 Hz, 5. 3 Hz), 2. 50 (3H, s), 4. 96 (1H, m), 5. 80 (2H, s), 6. 62 (2X dm), 7. 10 (1H, s), 7. 32 (2X dm), 7. 51 (2H, m) 95 ()-5- (4-Amino-3-methylphenyl)-8-methyi-7- (5-methyl- 140-144 72 1, 3, 4-thiadiazol-2-yl)-8, 9-dihydro-7H-1, 3-dioxolo- [4, 5-h] [2, 3] benzodiazepine Method MS : EI (70eV) : [M] + : 407, Hz. 392, 293, 266 CI : [M+H] + : 408, M+ : 407, m/z : 266 96 ()-5- (3-Amino-4-methylphenyl)-8-methyl-7-(5-methyl-125 70 1, 3, 4-thiadiazol-2-yl)-7H-1, 3-dioxolo- [4, 5-h] [2, 3] benzodiazepine Method 1H NMR (500 MHz) (DMSO-d6) 8 1. 17 (3H, d, 6. 5 Hz), 2. 10 (3H, s), 2-51 (3ES s), 2-72 (lX B dd, 14. 1 Hz, 9. 1 Hz), 3. 05 (lux dd, 14. 1 Hz, 4. 5 Hz), 5. 01 (2riz s), 5. 03 (lux m), 6. 07 (2riz dm), 6. 55 (1H. s), 6. 70 H, dd), 6. 83 (H, d, 1. 2 Hz), 7. 001H, d, 7. 8 Hz), 7. 06 (1X s) 97 ()-5- (3-Aminophenyl)-8-methyl-7- (5-methyl-1, 3, 4- 197-198 77 thiadiazol-2-yl)-8, 9-dihydro-7H-1, 3-dioxolo- (pro4 [4, 5-h] [2, 3] benzodiazepine Method 1H NMR (500 MHz) (DMSOd6) 8 1. 17 (3H, d, 6. 5 Hz), 2. 51 (3H, s), 2. 77 (1H, dd, 14. 2 Hz, B, C 8. 6 Hz), 3. 08 (1H, dd, 14. 2 Hz, 4. 3 Hz), 5. 06 (1H, m), 5. 24 (2riz s), 6. 07 (2H, dm), 6-54 (lux s), 6. 67 (1H. d), 6. 71 (H, d), 6. 74 (1H, d), 7. 06 (11-% s) 98 ()-l- (4-Aminophenyi)-4-methyl3- (5-methyl-1, 3, 4-180-184 84 thiadiazol-2-yi)-8-methox)4, 5-dihydro-3H- [2, 3] benzodiazepine Method MS : EI (70eV) : [Mj : 379, m/z : 364, 265, 238, 223 p 0 : : [M+H] + : 380, M+ : 37. 9, Sz : 223 99 (+)-5- (4-Aminophenyl)-8-methi-7- (5-methi-6H-1, 3, 4-154-157 85 thiadiazin-2-d)-8, 9-dihydro-7H-1, 3-dioxolo- [4, 5-h] [2, 3] benzodiaepine Method IH NN/a PMSC, c) 5 1. 20 (3H, d, 6. 5 Hz), 2. 10 (3X s), 2. 55 (1H, dd, 14. 0 Hz, 11 Hz), 2. 92 A (1X dm), 2. 92 (1H, dd, 14. 5 Hz), 3. 28 (1H, d, 14. 5 Hz), 5. 10 (1H, m), 5. 70 (2H, s), 6. 02 (2H, dm), 6. 55 (2H, dm), 7. 01 (1H s), 7. 38 (2X dm), 7. 60 (H, s) 100 | 5- (4-Aminophenyl)-7- (5, 6-dihydro-5-oxo-4H-1, 3, 4- 172-176 83 thkdiazm-2-yl)-8-methy8, 9-dihydro-7Hl, 3- dioxolo [4, 5-h] [2, 3] benzodiazepine 0 Number of Name Sol e (t of eld (%) Example recSstall. [a] D recrystall. Method 1H NMR (DMS5d6) õ 1. 16 (3I-I, d, 6. 5 Hz), 2. 49 (11-L dd, 14. 0 Hz, 10. 0 Hz), 2. 87 (1H, dd, A 14. 0 Hz, 5. 2 Hz), 3. 31 (2riz s), 4. 78 (IK m), 5. 68 (2riz s), 6. 05 (2H, dm), 6. 65 (1H, s), 6. 66 (H, dm), 7. 00 (1X s), 7. 32 (2U dm), 10. 5 (lR s) 101 (+)-5-(4-Aminophenyl)-8-methyl-7- (5-oxo-4, 5-dihydro- 263-264 47 1, 3, 4-thiadiazol-2-yl)-8, 9-dihydro-7H-1, 3-dioxolo- [4, 5-h] [2, 3] benzodiazepine Method iH NMR, (DM$-db) $ 1. 17 (3X d, 6. 5 Hz), 2. 58 (1H, dd, 14. 0 Hz, 10. 4 Hz), 2. 97 (1H, dd, C 14. 0 Hz, 5. 4 Hz), 4. 71 (lI m), 5. 65 (2H, s), 6. 04 (2H, dm), 6. 61 (2X dm), 6. 62 (lI s), 7. 01 (1if s), 7. 23 (2X dm), 11. 81 (1H, brs) MS : EI (70eV) : [M3+ : 395, n/z : 394, 306, 252 Ct : [M+HJ+ : 396, M+ : 395, n7Jz : 280 102 (R)-5- (4-Aminophenyl)-8-methyl-7- (5-methyl-1, 3, 4- 145-149 86 oxadiazol-2-yl)-8, 9-dihydro-7H-1, 3-dioxolb--663° (c=0. 5, Etch) [4, 5-h] [2, 3] benzodiazepine Method MS : EI (70eV) : N3+ : 377, wiz : 252 A Cl : [M+H] + : 378, [M3+ : 377, 7z/z : 252 103 ()-5- (4-Aminophenyl)-8-methyl-7- (2-methyl-3-oxo- 213 67 2, 3-dihydro-1, 2, 4-thiadiazol-5-yl)-8, 9-dihydro-7H-1, 3- (EtOH) dioxolo [4, 5-h] [2, 3] benzodiazepine Method 1H NMR (OMSad6) õ 1. 23 (3X d, 6. 5 Hz), 2. 70 (lX dd, 13. 8 Hz, 10. 2 Hz), 3. 03 (1H, dd, A 13. 8 Hz, 4. 2 Hz), 3. 06 (3X s), 4. 91 (1H, m), 5. 90 (2H, s), 6. 08 (2X dm), 6. 61 (1H, s), 6. 61 (2X dm), 7. 06 (1H, s), 7. 30 (2H, dm) 104 ()-5- (4-Aminophenyl)-7- (2-cyclopropyl-3-oxo-2, 3- 265-267 82 dihydro-1, 2, 4-thiadiazol-2-yl)-8-methyl-8, 9-dihydro- 7H-1, 3-dioxolo [4, 5-h] [2, 3] benzodiazepine Method 1HNMR (DMSO-d6) õ 0. 85 (4H, m), 1. 22 (3X d, 6. 5 Hz), 2. 75 (1IZ, dd, 14. 0 Hz, 10. 0 Hz), A 2. 75 (1H, m), 3. 02 (1H, dd, 14. 0 Hz, 4. 7 Hz), 4. 92 (1H, m), 5. 90 (2X s), 6. 07 (2riz, dm), 6. 60 (H, s), 6. 63 (2H, dm), 7. 04 (lX s), 7. 30 (2H, dm) 105 ()-5- (4-Aminophenyl)-7- (2-ethyl-3-oxo-2, 3-dihydro- 212-214 59 1, 2, 4-thiadiazol-2-yl)-8-meth-8, 9-dihydro-7H-1, 3- dioxolo [4, 5-h] [2, 3] benzodiazepine Method tHNE ((DC : 3) õ 1. 25 (3X t), 1. 27 (3H, d, 6. 5 Hz), 2. 80 (1H, dd, 14. 0 Hz, 9. 0 Hz), 3. 01 A (1H, dd, 14. 0 Hz, 4. 0 Hz), 3. 72 (2H, q), 4. 07 (2H, br), 5. 13 (1I-i, m), 6. 03 (2H, dm), 6. 65 (1H, s), 6. 67 (2riz dm), 6. 80 (1Hs s), 7. 37 (2H, dm) tE : EI (70eV) M+ : 423, ? 722 : 408, 279, 252, 160 CL : [M+H] + : 424, [M] : 423 106 ()-5- (4-Aminophenyl)-7- (4-carbo. thiazol-2-yl)-8->260 97 methyi-8, 9-dihydro-7H-1, 3-dioxolo- (dec) [4, 5-h] [2, 3] benzodiazepine Method MS : EI (70eV) : M+ : 422, m/z : 407, 279, 253 A Number of., P' (° Yield (%) Example Name Solvent of [ (XID recrystall. 107 (+)-5-(4-Aminophenyl)-8-methyl-7- (5-tetrazolyl)-8, 9->360 68 dihydro-7H-1, 3-dioxolo [4, 5-h] [2, 3] benzodiazepine Method MS : EI (70eV) : Rl+ : 363, rdz : 295, 294, 252 A Q : [M+H] + : 364, D/U+ : 363, nzlz : 295 108 ()-5- (4¢nophenyl)-8-methyl-7- (1, 2, 4-oxadiazol-3-| 124-126 48 yl)-7H-1, 3-dioxolo- [4, 5-h] [2, 3] benzodiazepine hydrochloride Method MS : EI (70e : D/il+ : 363, niz : 348, 253, 252 A Cl : [M+H] + : 364"X+ : 363, inlz : 252 109 (+)-5-(4-Aminophenyl)-8-methyl-7-(5-methyl-1, 2, 4- 130-135 74 oxadiazol-3-yl)-8, 9-dihydro-7H-1, 3-dioxolo- [4, 5-h] [2, 3] benzodiazepine Method MS : EI (70eV) (of the hydrochloride salt) : M+. : 377, inlz : 362, 278, 252 A CI : [M+H] + : 378, M"" : 377, m/z : 252 110 (+)-5- (4-Aminophenyl)-8-methyl-7- (2-methyl-thiazol-4-132-135 22 xi)-8, 9-dihydro-7H-1, 3-dioxolo- [4, 5-h] [2, 3] benzodiazepine Method MS : EI (70eV) : M+ ; : 392, into : 377, 279, 253, 252 C Cl : N+H] + : 393, M+ : 392 111 (+)-5- (4-Aminophenyl)-8-methyl-7- (2-pyrimidinyl)-8, 9-233-235 96 dihydro-7H-l, 3-dioxolo [4, 5-h] [2, 3] benzodiazepme (EtO Method tHNMR VtDMSO-d6) õ 1. 23 (3H, d, 6. 5 Hz), 2. 50 (1H, dd, 14. 0 Hz, 10. 0 Hz), 2. 89 (14. 0 Hz, A 4. 8 Hz), 5. 18 (1if m), 5. 71 (2riz s), 6. 03 (2H, dm), 6. 58 (2H, dm), 6. 60 (1H, s), 6. 60 (1EI, t, 4. 8 Hz), 7. 43 (H, s), 7. 30 (2H, dm), 8. 33 2H, d, 4. 8 Hz) 112 ()-5- (4-Aminophenyl)-7- (3-chloropyridazin-6-yl)-8- 164-166 94 methyl-8, 9-dihydro-7H-1, 3-dioxolo- (EtOH) [4, 5-h] [2, 3] benzodiazepine Method MS : EI (70eV) : + : 407/409, inlz : 392/394, 355, 279, 278, 253, 252 A CE : [M+Hl+ : 408/410, H+ : 407/409, and : 279 113 (i)-5-(4-Aminophen9-8-methi-7- (1H (2H)-1, 9, 4-178-181 64 triazol-3-yl)-8, 9-dihydro-7H-1, 3-dioxolo- [4, 5-h] [2, 3] benzodiazepine Method MS : EI (70eV) : SU+ : 362, rdz : 347, 279, 252 A 114 () _5_ (4_ophenyl)-8-methyl-7- (5-methyl-1H (2H)- 166-169 72 1, 2, 4-triazol-3-yl)-8, 9-dihydro-7H-1, 3-dioxolo- 0 Number | l Mp. (°C) | Yleld (%) . Name Solvent of recrystall. [4, 5-h] [2, 3] benzodiazepine Method MS : EI (70e : M+ : 376, nzlz : 361, 279, 252 A 115 (+)-5- (4-Aminopheng)-8-meth-7- (2-methi-2H-1, 2, 4-182-183 83 triazol-3-yl)-8, 9-dihydro-7H-13-di [4, 5-h] [2, 3] benzodiazepine Method MS : EI (70eV) : M+ : 376, niz : 361, 279, 252 A 116 ()-5_ (p, ophenyl)-8-methyl-7- (1-methyl 1H 1, 2, 4- 165-168 83 triazol-3-J1)-8, 9-dihydro-7H-1, 3-dioxolo- [4, 5-h] [2, 3] benzodiazepine Method MS : EI (70eV) : M+ : 376, Sz : 361, 253, 252 A 117 () _5_ (¢ophenyl)-8-methyl-7- (2, 5-dimethyl-2H-185-187 78 1, 2, 4-triazol-3-yl)-8, 9-dihydro-7H-1, 3-dioxolo- [4, 5-h] [2, 3] benzodiazepine Method MS : EI (70eV) : D/11+ : 390, m/z : 375, 279, 265, 252 A 118 ()-5- (4-Aminophenyl)-8-methyi-7- (1, 5-dimethyllH-197-200 85 1, 2, 4-triazol-3-yi)-8, 9-dihydro-7H-153-di [4, 5-h] [2, 3] benzodiazepine Method MS : EI (70e'V) : [M] +- : 390, onze : 375, 253, 252 C 119 (E)-5- (4-amino-3-methylphenyl)-8-methyl7- (5-methyl-158-160 83 1, 3, 4-thiadiazol-2-) 1)-8, 9-dihydro-7H-1, 3-dioxolo--515° (c=0. 38, CEiCl3) [4, 5-h] [2, 3] benzodiazepine MethodHNMR (DMSO-d.) 8 1. 18 (3EI, d, 5. 4 Hz), 2. 07 (s, 3H), 2. 47 (s, 3H), 2. 57 (dd, 1X1 ; 13. 7 Hz, B 10. 3 Hz), 2. 95 (dd, 1ES 13. 7 Hz, 4. 9 Hz), 4. 92 (m, 1H), 5. 2-5. 8 (br, 2EI), 6. 01 (s, br, 1H), 6. 06 (s, br, 1H), 6. 55 (s, 1H), 6. 64 (d, 1R 8. 2ho), 7. 04 (s, 1H), 7. 17 (d, IK 8. 2 Hz), 7. 25 (s, br, 1H) IVE : EI (70eV) : + : 407, rnlz : 392, 293, 278, 266 CI : [M+I-I] + : 408, SU+ : 407 Examples 120-131 General procedure for the synthesis of 2,3-benzodiazepines containing aoetylamino-phenyl group [00226] 2,3-benzodiazepines containing an aminophenyl group were dissolved in dichloromethane and stirred at room temperature with an excess of acetic anhydride. After completion of the reaction the mixture was washed with sodium hydrogen carbonate solution and water, then dried and concentrated.

-Table 11.

2, 3-benzodiazepine derivatives substituted with acetylammophenyl group Numberof Mp. (°C) Ylzeld (%) Example Name Solvent of n fctjo recrystall. f 120 ()-5- (4-Aceaminophenyl)-8-methyl-7- (5-methyl-176-179 65 thiazol-2-yi)-8, 9-dihydro-7H-1, 3-dioxolo- [4, 5-h] [2, 3] benzodiazepine 121 ()-5- (4-Acetylaminophenyl)-8-methyl-7- (4-methyl- 236-238 65 (50% thiazol-2-yl)-8, 9-dihydro-7H- 1, 3-dioxolo- EtOH% EtH Hz0) [4, 5-h] [2, 3] benzodiazepine 122 ()-5- (4-Acetylaminophenyl)-7- (4, 5-dihydro-thiazol-2- 211-213 96 yl)-8-methyl-8, 9-dihydro-7H-1, 3-dioxolo- (EtOI-i) [4, 5-h] [2, 3] benzodiazepine 123 (R)-5- (4-Acetyiaminophenyl)-8-methyl7- (2-thiazolyl)- 126 (rearrange 95 ment)-140° 8, 9-dihydro-7H-1, 3-dioxolo [4, 5-h] [2, 3] benzodIazepme ? i'0° (etc4 124 (S)-5- (4-Acetylaminophenyl)-8-methyl-7- (2-thiazolyl)- 124-128 95 8, 9-dihydro-7H-1, 3-dioxolo-+134° (c=0. 48, CHCl3) [4, 5-h] [2, 3] benzodiazepine 125 (: R)-5- (4-Acetlaminophenyl)-7- (4, 5-dihydro-thiazol-2- 143-145 95 yl)-8-methyl-8, 9-dihydro-7H-1, 3-dioxolo- +108° .... (c=0. 45, CHd3) [4, 5-h] [2, 3] benzodiazepine 126 (S)-5- (4-Acetylaminophenyl)-7- (4, 5-dihydro-thiazol-2-148-154 91 yl)-8-methyl-8, 9-dihydro-7H-1, 3-dioxolo--111° (c=048, Chu3) [4, 5-h] [2, 3] benzodiazepme 127 ()-5- (4-Acetylaminophenyl)-7- (4, 5-dihydro-oxazol-2- 124-128 44 51)-8-methyl-8, 9-dihydro-7H 1, 3-dioxolo- [4, 5-h] [2, 3] benzodiazepine (+)-5- (4-Acetylaminophenyl)-8-methyl-7- (2-162-163 96 (EtOH) pyrimidinyl)-8, 9-dihydro-7H-1, 3-dioxolo- (EtOE 3) Number of _ _ Example Name Solvent of..-'' recrystall. [4, 5-h] [2, 3] benzodiazepine 129 ()-5- (4-Acetlaminophenyl)-7 (3-chloro-pyridazin-6-164-170 78 yl)-8-methi-8, 9-dihydro-7H-1, 3-dioxolo- [4, 5-h] [2, 3] benzodiazepine 130 (R)-5- (GAcetalaminopheni)-8-methyl-7-(5-methi-276-277 73 0 1, 3, 4-thiadiazol-2-yl)-8, 9-dihydro-7H-1, 3-dioxolo- (MeOE3)-114° '' (c=0. 5, CHCl3) [4, 5-h] [2, 3] benzodiazepine' 131 ()-5_ (¢eo-3-methylphenyl)-8-methyl-7- (5- 258-262 63 methyl-1, 3, 4-thiadiazol-2-0-8, 9-dihydro-7H-1, 3- dioxolo [4, 5-h] [2, 3] benzodiazepine Example 132 (R)-8-Methyl-5-(3-methyl-4-nitrophenyl)-7-(4,5-dihydro-thiaz ol-2-yl)-8,9-dihydro-7H-1,3- dioxolo[4,5-h][2,3]benzodiazepine Step A <BR> <BR> <BR> <BR> <BR> <BR> [00227] (R)-8-Methyl-5-(3-methyl-4-nitrophenyl)-7-thiocarbamoyl-8,9- dihydro-7H-1,3-dioxolo[4,5-<BR> <BR> b][2,3]benzodiazepine [00228] This compound was prepared from starting compound XE according to the process described for starting compound I. Mp.: 123-125°C. Yield: 70%.

Step B [00229] The product of Step A was transformed into the title compound according to the procedure described in Example 9. Mp.: 130-135°C. Yield: 81%..

Example 133 (R)-8-Methyl-5-(3-methyl-4-nitrophenyl)-7-(thiazol-2-yl)-8,9 -dihydro-7H-1,3-dioxolo[4,5- h][2,3]benzodiazepine [00230] The title compound was obtained from the intermediate described under Step A of Example 132 according to a method described in Example 1. Mp.: 138-142°C. Yield: 55%.

Example 134 <BR> <BR> <BR> <BR> (R)-7- (5-Ethyl-1, 3, 4-thiadiazol-2-yl)-8-methyl-5- (3-methyl-4-nitrophenyl)-8, 9-dihydro-7H- 1,3-dioxolo[4,5-h][2,3]benzodiazepine Step A [00231] (R)-8-Methyl-5-(3-methyl-4-nitrophenyl)-8,9-dibydro-7H-1,3-d ioxolo4, 5- h][2,3] benz odiazepine-7-carbothioyl chloride [00232] This compound was prepared from starting compound XXXI according to the <BR> <BR> <BR> method described for starting compound XI. Mp. : 193-196°C. Yield: 85%. [α]D : -500. 0° (c=0.5 ; CHCl3).

Step B [00233] 2.50 g (6.0 mmol) of the compound obtained in Step A was reacted-with 1.28 g (21.3 mmol) of propionyl hydrazide in 10 ml of dimethylformamide at 70°C for 2 h. The cooled reaction mixture was poured onto water and the resulting precipitate was collected by filtration.

This wet substance was further reacted in 24 ml of ethanol with 0.5 ml conc. hydrochloric acid at boiling point for 1 h. Solvent was evaporated and the residue was dissolved in dichloromethane and extracted with sodium bicarbonate solution and water. Evaporation of the solvent gave the crude title product which was purified by column chromatography using a mixture of n-hexane- ethyl acetate (1: 1) as eluent, giving 1.15 g (yield: 49%) of the product. Mp.: 129-130°C.

Example 135 <BR> <BR> <BR> <BR> (R)-8-Methyl-5-(3-methyl-4-nitrophenyl)-7-(5-propyl-1,3,4-th iadiazol-2-yl)-8,9-dihydro-<BR> <BR> <BR> 7H-1, 3-dioxolo[4,5-h][2,3] benzodiazepine [00234] The title compound was obtained according to the method described in Example 134 but using butynchydiazide. Mp.: 143-145°C. Yield: 73%. [a] D : +343. 3° (c=0. 5 ; CHCl3).

Example 136 (R)-8-Methyl-5-(3-methyl-4-nitrophenyl)-7-(1,3,4-thiadiazol- 2-yl)-8,9-dihydro-7H-1,3- dioxolo[4,5-h][2,3]benzodiazepine <BR> <BR> <BR> <BR> Step A<BR> [00235] (R)-8-Methyl-5-(3-methyl-4-nitrophenyl)-8,9-dibydro-7H-1,3-d ioxolo[4,5- h][2,3] benzodiazepine-7-carbothiobydrazide [00236]'The Step A intermediate of Example 134 was transformed into the carbothiohydrazide according to a method described for starting material XVIII. Mp. : 109- 115°C. Yield: 91%. [a] D :-276. 5' (c=0.5 ; CHCl3).

Step B [00237] The compound of Step A was reacted with triethyl orthoformate and a catalytic amount of hydrochloric acid similarly to a method described in Example 25, to give the title product. Mp.: 182-189°C. Yield: 92%. [a] D : +356. 0° (c=0.5 ; CHCb).

Example 137 <BR> <BR> <BR> <BR> (R)-8-Methyl-5-(3-methyl-4-nitrophenyl)-7-(5-methoxymethyl-1 ,3,4-thiadiazol-2-yl)-8,9-<BR> <BR> <BR> dihydro-7H-L3-dioxolo [4*5-h] [23] benzodiazepine [00238] The compound of Step A of Example 136 (2.07 g, 5.0 mmol) was reacted in 10 ml of ditnethylformamide with 1.86 g (5.5 mmol) of pentacblotophenol methoxyacetate at 50°C for 2 h. The reaction mixture was diluted with water and the resulting precipitate was isolated by filtration. This wet intermediate was taken up in ethanol (24 ml), 0.50 ml of conc. hydrochloric acid was added and it was boiled for 1 h. Evaporation of the solvent gave a residue, which was dissolved in methylene chloride and the solution was washed with a 5% sodium carbonate solution and water. Evaporation of the solvent resulted in the crude title product that was purified with column chromatography ; a mixture of n-hexane-ethyl acetate (2: 1) was used as eluent to give 2.21 g of the pute product. Mp.: 153-155°C. Yield: 91%. [ojo : +317. 5° (c=0.5 ; CHCl).

[00239] The compounds of Examples 138-148 were prepared analogouslyto the method described in Example 137 using the appropriate activated carboxylic acid derivatives as reagents (e. g.: acyl chloride, acid anhydride, pentachlorophenol ester, N-hydroxysuccinimide ester of the corresponding carboxylic acids).

Example 138 (R)-8-Methyl-5-(3-methyl-4-nitrophenyl)-7-(5-isopropyl-1,3,4 -thiadiazol-2-yl)-8,9-dihydro- 7H-1,3-dioxolo[4,5-h][2,3] benzodiazepine [00240] Mp.: 130-133°C. Yield: 90%.

Example 139 (R)-7-(5-Cyclopropyl-1,3,4-thiadiazol-2-yl)-8-methyl-5-(3-me thyl-4-nitrophenyl)-8, 9- dihydro-7H-1. 3-dioxolo[4,5-h][2,3] benzodiazepine [00241] Mp.: 126-130°C. Yield: 93%.

Example 140 (R)-7-(5-Hydroxymethyl-1,3,4-thiadiazol-2-yl)-8-methyl-5-(3- methyl-4-nitrophenyl)-8, 9- dihydro-7H-l, 3-dioxolo [4, 5-h] [2, 3] benzodiazepme [00242] Mp.: 142-145°C. Yield: 67%.

Example 141 <BR> <BR> <BR> <BR> (R)-7-(5-Acetoxymethyl-1,3-4-thiadiazol-2-yl)-8-methyl-5-(3- methyl-4-nitrophenyl)-8,9-<BR> <BR> <BR> dihydro-7H-1, 3-dioxolo[4,5-h][2,3]benzodiazepine [00243] Mp.: 110-115°C. Yield: 97%.

Example 142 (R)-7-(5-Cyanomethyl-1,3,4-thiadiazol-2-yl)-8-methyl-5-(3-me thyl-4-nitrophenyl)-8,9- dihydro-7H-t3-dioxolo [4, 5-h] [2, 3] benzodiazepme [00244] Mp.: 118-122°C. Yield: 98%.

Example 143 (R)-8-IIeth 1-5-(3-methyl-4-nitrophenyl)-7-(5-methylthiomethyl-1,3,4-thi adiazol-2-yl)-8,9- dihydro-7H-1, dihydro-7H-1,3-dioxolo[4,5-h][2, 3] bei ! Zodiazepme [00245] Mp.: 132-134°C. Yield: 96%.

Example 144 (R)-7-(5-Ethoxycarbonyl-1,3,4-thiadiazol-2-yl)-8-methyl-5-(3 -methyl-4-nitrophenyl)-8,9 dihvdro-7H-1, 3-dioxolo [4, 5-h] [2, 3] benzodiazepine [00246] Mp.: 115-118°C. Yield: 80%. [ (x] D : +140. 3° (c=0.5 ; CHCl3).

Example 145 (R)-7-(5-Benzyloxycarbonyl-aminomethyl-1,3,4-thiadiazol-2-yl )-8-methyl-5-(3-methyl-4 nitrophenyl)-8,9-dihydro-7H-1,3-dioxolo[4,5-h][2,3]benzodiaz epine [00247] Mp.: 240-243°C. Yield: 95%.

Example 146 (R)-8-Methyl-5-(3-methyl-4-nitrophenyl)-7-{5-[1-(1E)-propen- 1-yl]-1,3,4-thiadiazol-2-yl 8, 9-dihvdro-7H-1. 3-dioxolo [4, 5-h] [2, 3] benzodiazepine [00248] Mp.: 232-237°C. Yield: 28%. [oJD : -359. 2° (c=0. 4; CHC13).

Example 147 (R)-7-(5-Hexyl-1,3,4-thiadiazol-2-yl)-8-methyl-5-(3-methyl-4 -nitrophenyl)-8,9-dihydro- 7H-1,3-dioxolo[4,5-h][2, 3] benzodiazepine [00249] Mp.: 217-224°C. Yield: 66%.

Example 148 (R (5. 6-Dihydro-5-oxo-4H-1,3,4-thiadiazin-2-yl)-8-methyl-5-(3-meth yl-4-nitrophenyl) 8,9-dihydro-7H-1,3-dioxolo[4,5-h][2,3]benzodiazepine [00250] The title compound was prepared from the compound obtained in Step A of Example 136 applying the method described in Example 43, however pentachlorophenol chloroacetate was used as alkylating agent. Mp.: 207-211°C Yield: 70%. [Ct] D : +378. 5° (c=0.5 ; CHCl3).

Example 149 (R)-8-Methyl-5-(4-nitrophenyl)-7-(1,3,4-oxadiazol-2-yl)-8,9- dihydro-7H-1,3-dioxolo[4, 5- hl [2, 3] benzodiazepine [00251] The title compound was obtained from (R)-N'- {8-methyl-5- (4-nitrophenyl)-8, 9- dihydro-7H-1,3-dioxolo [4,5-h] [2, 3] benzodiazepine-7-carbothioyl}-formic hydrazide, according to the method described in Example 45. Mp.: 145-147°C. Yield: 35%, [clD :-604. 0° (c=0. 5; GHC13).

Example 150 <BR> <BR> <BR> <BR> <BR> (~)-8-Methyl-5-(4-nitrophenyl)-7-(1,2,3,4-thiatriazol-5-yl)- 8,9-dihydro-7H-1,3-dioxolo[4,5-<BR> <BR> <BR> h] [2, 3] benzodiazepine [00252] A solution of 1.00 g (2.5 mmol) of starting material XVIII, 0. 37 ml (4.6 mmol) of ttifluoroacetic acid in 10 ml of formamid was stirred at 25°C for 5 min. Then a solution of 0. 16 g (2.5 mmol) of sodium nitrite in 0.30 rnl of water was added dropwise. After 0.5 h the reaction mixture was diluted with water and the precipitate formed was filtered off, washed with water and dtied to yield 0.92 g (90%) of the title compound. Mp.: 109-110°C.

Example 151 (~)-8-Methyl-5-(4-nitrophenyl)-7-(2-methyl-1,3-oxazol-5-yl)- 8,9-dihydro-7H-1, 3- dioxolo[4,5-h][2,3]benzodiazepine Step A [00253] (~)-N'-{8-methyl-5-(4-nitrophenyl)-8,9-dihydro-7H-1,3-dioxol o[4,5- [00254] A solution of 1.5 g (4.6 mmol) of (+)-8-methyl-5-(4-nitrophenyl)-8, 9-dihydro-7H- 1, 3-dioxolo [4,5-h] [2,3] benzodiazepine, 0.5 g (4.6 mmol) of N-acetylglycine and 1.0 g (5.0 mmol) of 1, 3-dicyclohexylcatbodiimid was stirred in 15 ml of dichloromethane at 25°C for 3 h. The precipitated 1, 3-dicyclohexylurea was filtered off and the filtrate was evaporated to dryness. The crude product was purified by column chromatography using a mixture of ethyl acetate-hexane (1: 1) as eluent to yield 1.1 g (58%) of the title compound.

Step B [00255] 0.74 g (2.8 mmol) of triphenylphosphine was dissolved in 10 ml of dichloromethane and a solution of 0.2 ml (2.8 mmol) of bromine in 1 ml of dichloromethane was added. After 30 min a solution of 1.0 g (2.4 mmol) of the compound prepared in Step A and 1.0 ml (7.1 mmol) of triethylamine in 5 ml of dichloromethane was added and the mixture was boiled under nitrogen for 3 h. The resulting solution was washed with water, dried and concentrated to dryness. The crude product was purified by column chromatography using a mixture of ethyl acetate-hexane (1: 1) as eluent to yield 0.6 g (62%) of the title compound. Mp.: 203-205°C.

Example 152 <BR> <BR> <BR> <BR> <BR> ()-7- (2. 4-dimethyl-t3-oxazol-5-yl)-8-methyl-5- (4-nitrophenyl)-8. 9-dihydro-7H-1. 3-<BR> )-8,9-dih<BR> <BR> <BR> dioxolo [4, 5-h] [2, 3] benzodiazepine [00256] The title compound was obtained according to the method described in Example 151, but using (~)-N-{2-[8-methyl-5-(4-nitrophenyl)-8,9-dihydro-7H-1, 3-dioxolo [4, 5- h] [2, 3] benzOdiazepine-7-yl]-1-nethyl-2-oxoethyl}-acetarIiide as intermediate. Mp. : 76-78°C ; yield: 68%.

Example 153 (R)-5- (3-Chloro-4-nitrophenyl)-8-methyl-7- (5-methyl-1, 3-thiadiazol-2-yl)-8, 9-dihydro- 7H-1, 3-dioxolo [4, 5-h] [2, 3] benzodiazepine [00257] Step A [00258] (R)-7-(tert-Butoxycarbonyl)-5-(3-chloro-4-nitrophenyl)-8-met hyl-7H-1,3-dioxolo[4,5- h] '[2,3]benzodiazepine [00259] The compound was prepared according to a synthesis described in literature (Anderson et al., J. Am Chem Soc 117: 12358 (1995) ) using 3-chloro-4-nitrobenzaldehyde and tert-butyl carbazate as key agents. Mp.: 160-162°C.

Step B (R)-5-(3-Chloro-4-nitrophenyl)-8-methyl-8,9-dihydro-7H-1,3-d ioxolo[4,5-b][2,3]benzodiazepine [00260] The compound obtained in Step A (8.2 g; 18.2 mmol) was dissolved in ethyl acetate, containing 12% hydrochloric acid (82 ml). The solution was maintained at RT for 3 h.

Then the solvent was evaporated and the residue was dissolved in ethyl acetate and washed with saturated sodium hydrogencarbonate solution and water. Evaporation yielded 5.3 g (81%) of the title product. Mp.: 165-170°C. [a] D : +65. 0° (c=0.5 ; CHCl3).

Step C (R)-5-(3-Chloro-4-ntrophenyl)-8-methyl-8,9-dihydro-7H-1,3-di oxolo[4,5-b][2,3]benzodiazepine-7-carbothioyl chloride [00261] The compound was prepared from the intermediate obtained in Step B according to the method described for starting material XI. Mp.: 132-134°C. Yield: 88%. [OC] D : - 533. 0° (c=0.5 ; CHCb).

Step D [00262] The title compound was obtained from the compound prepared in Step C according to a method described in Method B of Example 28. Mp.: 151-152°C. Yield: 89%. [a] D : +284. 1° (c=0.5 ; CHCl3).

Example 154 (R)-5-(3-Chloro-4-nitrophenyl)-8-methyl-7-(5-methoxymethyl-1 ,3,4-thiadiazol-2-yl)-8,9- dihydro-7H-1,3-dioxolo[4,5-h][2,3]benzodiazepine Step A (R)-5-(3-Chloro-4-nitrophenyl)-8-methyl-8,9-dihyolro-7H-1,3- dioxolo[4,5-b]benzodiazepine-7- carbothioloydrazide [00263] The compound obtained in Step C of Example 153 was transformed into the title compound according to a method described fot starting material XVIII. Mp.: 126-127°C ; yield: 85%.

Step B [00264] The compound obtained in Step A was used to prepare the title compound according to the met hod described in Example 137. Mp.: 208-210°C ; yield : 65%. [α]D: +470. 6° (c=0.5 ; CHCl3).

Example 155 <BR> <BR> <BR> <BR> <BR> ()-8-Methyl-7- (3-methyl-isoxazol-5-yl)4-nitrophenyl)-8, 9-dihydro-7H-1, 3-<BR> <BR> <BR> dioxolo[4,5-h][2,3]benzodiazepine Step A ()-1- 5-(4-nitrophenyl)-8,9-dihydro-7H-1,3-dioxolo[4,5-b]benzodiaz epin-7-yl}-butane-1,3- dione [00265] A solution of 4.0 g (12.3 mmol) of ()-8-methyl-5- (4-nitrophenyl)-8, 9-dihydro- 7H-1, 3-dioxolo [4,5-h] [2,3] benzodiazepine, 0.52 ml (13.5 mmol) of diketene in 80 ml of toluene was stirred at 80°C for 3 h. The reaction mixture was washed with water, dried and concentrated.

The residue was triturated with diisopropyl ether to yield 4.0 g (80%) of the title compound. Mp.: 169-171°C.

Step B (~)-4-{8-Methyl-5-(4-nitrophenyl)-8,9-dihydro-7H-1,3-dioxolo [4,5-b][2,3]benzodiazepin-7-yl}-4-thioxo- butan-2-one [00266] A solution of 3.0 g (7.2 mmol) of a compound of Step A 2.4 g (5 mmol) of Lawesson's reagent in 500 tnl of toluene was stirred at reflux temperature for 4 h. Then the reaction mixture-was filtered and the solvent evaporated. The crude product was purified by column chromatography using a mixture of ethyl acetate-hexane (1: 3) as eluent to yield 2.1 g (70%) of the title compound. Mp.: 178-185°C.

Step C [00267] A solution of 1.9 g (4.5 mmol) of the compound obtained in Step B and 0. 6 g (9.0 mmol) hydroxylamine hydrochloride was stirred in 20 ml of ethanol and heated at reflux for 3 h. The reaction mixture was diluted with water and the precipitate formed was filtered off. The crude product was purified by column chromatography using a mixture of ethyl acetate-hexane (1: 3) as eluent to yield 0.60 g (32%) of the title compound. Mp.: 179-182°C.

Example 156 (R)-5-(3,5-Dimethyl-4-nitrophenyl)-8-methyl-7-(5-methyl-1,3, 4-thiadiazol-2-yl)-7H-1,3- dioxolo[4,5-h][2,3]benzodiazepine Step A (R)-7-(tert-Butoxycarbonyl)-5-(3,5-dimethyl-4-nitrophenyl)-8 ,9-dihydro-7H-1,3-dioxolo[4, 5- h][2,3]benzodiazepine [00268] The compound was prepared according to a synthesis described in literature (Anderson etat, J. Am C2vem Soc 117: 12358 (1995) ), however as key reagents 3, 5-dimethyl-4- nittobenzaldehyde and tett-butyl carbazate were used. Mp.: 222-223°C. [a] D:-443. 0° (c=0. 5 ; CHCl3).

Step B (R)-5-(3,5-Dimethyl-4-nitrophenyl)-8-methyl-8,9-dihydro-7H-1 ,3-dioxolo[2,3]benzodiazepine [00269] The compound obtained in Step A was subjected to hydrolysis according to a method described in Example 153 under Step B. Mp.: 193°C ; yield: 88%. [a] D : +181° (c=0. 5 ; cera3).

Step C (R)-5-(3,5-Dimethyl-4-nitrophenyl)-8-methyl-8,9-dihydro-7H-1 ,3-dioxolo[4,5-b][2,3]benzodiazepine-7- carbothioyl chloride [00270] The compound obtained in Step B was transformed into the title carbothioyl derivative according to a method described for starting compound XI. Mp.: 216°C ; yield: 82%.

[a] D :-389' (c= : 0. 5; (CHCl3).

Step D [00271] The title compound of this example was prepared from compound obtained in Step C according to Method B described in Example 28. Mp.: 235°C ; yield : 86%. [oclD : +221° (c=0. 5 ; CHCb).

Example 157 <BR> <BR> <BR> <BR> <BR> (R)-5- (3, 5-Dimethyl-4-nitrophenyl)-8-methyl-7-(5-methyl-1, 3, 4-oxadiazol-2-yl)-8, 9-<BR> <BR> <BR> <BR> dihydro-7H-1,3-dioxolo4,5-h][2,3]benzodiazepine [00272] The thiosemicarbazide type intermediate of Step D of Example 156 was treated with mercury (II) acetate for 16 h according to a procedure described in Example 45. Mp.: 132- 133°C ; yield : 90%. [a] D :-436° (c=0.5 ; CHC13).

Example 158 <BR> <BR> <BR> <BR> <BR> (Rl- (3, 5-Dimethyl-4-nitrophenyl)-8-methyl-7- (2-thiazolyl-8, 9-dihydro-7H-1, 3-<BR> <BR> <BR> dioxolo 4, 5-h] [2, 3] benzodiazepine Step A (R)-5-(3,5-Dimethyl-4-nitropheny)-8-methyl-7-bhiocarbamoyl-8 ,9-diydro-7H-1,3-dioxolo[4,5- b][2,3]benzodiazepine [00273] This intermediate was prepared from the compound obtained in Step B of Example 156 by a method described for starting material I, however, during the reaction significant hydrolysis of the title product to the corresponding urea derivative was noticed as well. Title compound was isolated by column chromatography using a mixture of hexane-ethyl acetate (3: 1) as eluent. Mp. 298-230°C ; yield : 18%.

Step B [00274] Intermediate compound obtained in Step A was reacted with bromoacetaldehyde diethyl acetal as described in Example 1. Mp.: 167°C ; yield: 46%.

Example 159 (R)-8-Methyl-7- (2-methyl-3-oxo-2, 3-dihydro-1, 2, 4-thiadiazol-5-yl)-5- 4-nitrophenyl)-8, 9- dihydro-7H-1,3-dioxolo[4, 5-h] [2,3]benzodiazepine <BR> <BR> <BR> <BR> <BR> <BR> <BR> Step A<BR> Step A (R)-Pbenyl-(8-metbyl-5-(4-nitropbenyl)-8,9-dibydro-7H-1,3-di oxdo[4,5-b][2,3]benzodiazepine-3-carbotbioyl)- carbamate [00275] Prepared according to the procedure described for starting compound XXVI, however, from (R)-8-methyl-5-(4-nitrophenyl)-8,9-dihydro-7H-1,3-dioxolo [4,5- h] [2, 3] benzodiazepine. The crude product was used without further purification.

Step B (R)-1-Methyl-3-{8-methyl-5-(4-nitropbenyl)-8,9-dibydro-7H-1, 3-dioxdo[4,5-b][2,3]benzodiazepine-7- carbotbioyl}-urea [00276] Intermediate of Step A was reacted with. methylamine according to the method described for the tacemic starting compound XXVII. Mp.: 164°C ; yield: 63%.. [α]d: -526° (c=0. 5; CHCl3).

Step C [00277] The compound of Step B was reacted with bromine according to a procedure described in Example 46 to give the title product. Mp. : 177-180°C ; yield : 98%. [oc] D : (c=0. 5; ICI3).

Example 160 <BR> <BR> <BR> <BR> <BR> <BR> <BR> ()-7- (5, 5,5-Dimethyl-4-oxo-4,5-dihydro-thiazol-2-yl)-8-methyl-5-(4-n itrophenyl)-1,3-<BR> <BR> <BR> <BR> <BR> <BR> dioxolo [4, 5-h] [2, 3] benzodmzepme [00278] To a suspension of 1.20 g (2.86 mmol) of starting material I in 10 ml of dimethylformamide 1. 67 g (8.58 mmol) of ethyl-a-bromoisobutyrate were added. The mixture was stored at 80°C for 1 h and at 100-110°C for 23 h. The solution was diluted with water and the separated oily substance was extracted into dichloromethane. After washing and drying the solvent was evaporated and the residue was purified by column chromatography, using a mixture of hexane-ethyl acetate (1: 1) as eluent. Evaporation of the fractions containing the main product gave 0.80 g of the title compound as a gum.

Example 161 <BR> <BR> <BR> <BR> (R)-8-Methyl-5-(4-nitrophenyl)-7-(1,2,3-thiadiazol-5-yl)-8,9 -dihydro-7H-1,3-dioxolo[4, 5-<BR> <BR> <BR> hl [2, 3] benzodiazepine [00279] To an ethereal solution containing diazomethane in high excess, a solution of 2.42 g (3.0 mmol) of starting material XII in 40 ml of tetrahydrofurane was added dropwise at- 15°C. The solution was kept at room temperature for 5 days, when TLC showed full conversion.

Evaporation gave a residue which was purified by column chromatography, using a mixture of hexane-ethyl acetate (3: 1) as eluent. 2. 13 g of the title product was resulted. Mp.: 175-176°C/ [a] D :-96° (c=0. 5, CHCl3).

Example 162 <BR> <BR> <BR> <BR> (R)-5-(2-Bromo-3-methyl-4-nitrophenyl)-8-methyl-7-(5-methyl- 1,3,4-thiadiazol-2-yl)-8, 9-<BR> <BR> dihydro-7H-1, dihydro-7H-1,3-dioxolo[4,5-h][2,3]benzodiazepine Step A (R)-5 (2-Bromo-3-metbyl-4-nitropbenyl)-7-(tert-butoxycarbonyl)-8-m ethyl-8,9-dibydro-7H-1,3-dioxdo[4,5- b][2,3]benzodiazepine [00280] The compound was prepared using 2-bromo-3-methylbenzaldehyde and tert- buty3carbazate according to a method published in the literature (Anderson et al., J. Am Cbem SoG 117 : 12358 (1995)).

[00281] MS : EI (70eV) : H+ : 517/519, iz. 417/419, 376/378,57 [00282] CI:[M+H]+: 518/520 Step B (R)-5-(2-Bromo-3-metbyl-4-nitropbenyl)-8-metbyl-8,9-dibydro- 7H-1,3-dioxdo[4,5-b][2,3]benzodiazepine [00283] Compound obtained in Step A was hydrolyzed according to method disclosed in Example 153, Step B.

[00284] MS : EI (70e V) : X+ : 417/419, m/z: 402/404, 374/376,338, 160 [00285] CI:[M+H]+: 418/420, [M]+: 417/419 Step C (R)-5-(2-Bromo-3-metbyl-4-nitrophenyl)-8-mebyl-8,9-dibydro-7 H-1,3-dioxdo[4,5-b][2,3]benzodiazepine-7- carbotbioyl doloride [00286] The compound obtained in Step B was transformed into the title compound according to a method described for starting material XI.

[00287] ME : EI (70eV) : [M]+. : 495/497, m/z: 460/462, 401/403,355/357 [00288] Cl : [M+H] + : 496/498/500, .-460/462 Step D [00289] The title compound obtained in Step C was further reacted with acetic hydrazide according to a method described in Method B of Example 28 to give the title compound as a foam.

[00290] MS : EI (70eV) + : 515/517, m/z: 500/502, 401/403,59 [00291] CI : [M+H]+ : 516/518 [00292] Table 12. <BR> <BR> <P> 2, 3-Benzodiazepines containing aminophenyl group<BR> <BR> <BR> <BR> (The 1H NMR spectra were recorded at 500 MHz unless stated otherwise) Yield (%) Example f Name Solvent of [a] D recrystall. 163 g-5- (4-Amino-3-methyiphen34)-8-methyl-7- (4, 5- 125-128 53 dihydro-thiazol-2-yl)-8, 9-dihyb7H-1n3-dioxolo--282. 0° (c=0. 5, CHCI3) [4, 5-h] [2, 3] benzodiazepine MethodHNMR (DMSO-) d 1. 10 (3H, d, 6. 0 Hz), 2. 06 (3H, s), 2. 53 (1H, dd, 14. 0 Hz, 10. 0 Hz), B 2. 86 (IK dd, 14. 0 Hz, 4. 5 Hz), 3. 0-3. 2 (2H, m), 3. 93 (lX m), 4. 05 (1EI, m), 4. 85 (1H, m), 5. 35 (2H, s), 6. 04 (IK s), 6. 07 (1H, s), 6. 55 (1H, s), 6. 61 (1H, d, 8. 5 Hz), 6. 98 (11-1 s), 7. 13 (1H, d, br, 8. 5 Hz), 7. 22 H, s, br) 164-5- (G ncF3-methylphenyl)-8-methyl-7-(thiazol-2-124-127 86 xi)-8, 9-dihydro-7H-133-dioxolo--619. 5° (c=0. 5, CHCt3) [4, 5-h] [2, 3] benzodiazepine Method 1H NMR (DMSOdó) d 1. 16 (3H, d, 6. 0 Hz), 2. 09 (3H, s), 2. 59 (1H, dd, 14. 0 Hz, 10. 5 Hz), B 2. 95 (1H, dd, 14. 0 Hz, 5. 5 Hz), 5. 01 (1H, m), 5. 47 (2H, s, br), 6. 03 (1H, d, 1. 1 Hz), 6. 08 (1if d, 1. 1 Hz), 6. 58 (1H, s), 6. 64 (1H, d, 8. 0 Hz), 6. 83 (1H, d, 3. 5 Hz), 7. 05 (1H, s), 7. 21 (1H, dd, 8. 0 Hz, 2. 0 Hz), 7. 28 (1R d, 3. 5 Hz), 7. 31 (1H, d, 2. 0 Hz) * see tbe general prooedures giren before Examples 60-118 for reduction of tbe nitro groups of utrious 2,3-benzodiazepines. 0 Number of., P- (° Yield (%) Example rec stall. [° rys 165-5- (4-Amino-3-methylphenyl)-7- (5-ethyl-1, 3, 4- 129-133 90 - 534. 9° thiadiazol-2-yl)-8-methyl-8, 9-dihydro-7H-1, 3-dioxolo-EtOE3) (c =0. 5, GHG13) (c=0. 5, CHC13) [4, 5-h] [2, 3] benzodiazepine Method 1HNMR (DMSO-d6) d 1. 20 (3H, d, 6. 1 Hz), 1. 23 (3H, t, 7. 6 Hz), 2. 07 (3H, s), 2. 58 (1H, dd, 14. 0 Hz, 10. 6 Hz), 2. 85 (2H, q, 7. 6 Hz), 2. 95 (1H, dd, 14. 0 Hz, 5. 4 Hz), 4. 93 (1H, m), 5. 50 (2H, s, br), 6. 03 (1H, d, 1. 0 Hz), 6. 08 (1H, d, 1. 0 Hz), 6. 57 (1H, s), 6. 63 (1H, d, 8. 4 Hz), 7. 06 (1H, s), 7. 20 (1H, dd, 8. 4 Hz, 2. 1 Hz), 7. 25 (1H, d, 2. 1 Hz) MS : EI (70eV) : M+ : 421, m/z : 406, 293, 266 CL : [M+H] + : 422, M+ : 421 166 (R)-5- (4-Amino-3-methylphenyl)-8-methyl-7- (5-propyl-150-154 56 - 516. 0° 1, 3, 4-thiadiazol-2-yl)-8, 9-dihydro-7H-1, 3-dioxolo [4, 5- (EtOH), ; , (c=0. 5, Cn. U3) h] [2, 3] benzodiazepine Method 1HNMR (T) MSO-aL) d 0. 91 (t, 7. 5 Hz), 1. 20 (3H, d, 6. 1 Hz), 1. 66 (2H, m), 2. 08 (3H, s), 2. 58 (1H, dd, 13. 8 Hz, 10. 6 Hz), 2. 80 (2H, t, 7. 2 Hz), 2. 96 (1H, dd, 13. 8 Hz, 5. 1 Hz), 4. 94 (11- m), 5. 50 (2H, s), 6. 03 (1H, s), 6. 08 (1H, s), 6. 57 (1H, s), 6. 64 (1H, d, 8. 3 Hz), 7. 05 (1H, s), 7. 19 (lit, dd, 8. 3 Hz, 2. 1 Hz), 7. 25 (1H, d, 2. 1 Hz) 167 (R)-5- (4-Pmino-3-methylphenyl)-8-methyl-7- (1, 3, 4- 143-148 63 - 527. 3 ° thiadiazol-2-yl)-8, 9-dihydro-7H-1, 3-dioxolo- (gtOAc) 527 3 o [4, 5-h] [2, 3] benzodiazepine Method 1HNMR (DMSO-db) d 1. 21 (3H, d, 6. 1 Hz), 2. 08 (3H, s), 2. 61 (1H, dd, 13. 6 Hz, 10. 6 Hz), B 3. 00 (IK dd, 13. 6 Hz, 5. 0 Hz), 5. 00 (1H, m), 5. 52 (2H, s, br), 6. 03 (1ES, d, 0. 7 Hz), 6. 08 (1H, d, 0. 7 Hz), 6. 58 (1X s), 6. 64 (1X d, 8. 3 Hz), 7. 07 (lI s), 7. 20 (1if dd, 8. 3 Hz, 2. 1 Hz), 7. 28 (1H, d, 2. 1 Hz), 8. 78 (1H, s) MS : EI (70eV) : N3+ : 393, m/z : 378, 266 Cl : : [M+H3+ : 394, W3+ : 393 168 (R)-5- (4-Amino-3-methyiphenyl)-8-methyl-7- (5-171-172 86 methoxymethyl-1, 3, 4-thiadiazol-2-yl)-8, 9-dihydro-7H- (EtOH)-540. 0° (c=0. 5, CHCl3) 1, 3-dioxolo- [4, 5-h] [2, 3] benzodiazepine Method 1H NE (DMSO-aN) d 1. 21 (3H, d, 6. 1 Hz), 2. 08 (3X s), 2. 60 (1H, dd, 14. 0 Hz, 11. 0 Hz), B 2. 97 (1X dd, 14. 0 Hz, 5. 4 Hz), 4. 57 (1X d, 12. 7 Hz), 4. 60 (1H, d, 12. 7 Hz), 4. 98 (IK m), 5. 3 - 5. 8 (2H), 6. 03 (1H, s), 6. 08 (1H, s), 6. 58 (1H, s), 6. 65 (IK d, 8. 6 Hz), 7. 06 (1H, s), 7. 21 (1H, dd, 8. 6 Hz, 2. 2 Hz), 7. 26 (H, d, 2. 2 Hz) 169 (R)-5-(4-Aniino-3-methylphen)-7-(5-isopropyl-1, 3, 4- 134-140 83 tluadiazol-2-yl)-8-methyl-8, 9-dihydro-7H-1, 3--518. 8° (c=0. 5, CHC13) dioxolo [4, 5-h] [2, 33benzodiazepine Method iHNMR (DMSOa ;) d 1. 20 (3H, d, 6. 2 Hz), 1. 26 (3ER d, 6. 9 Hz), 1. 28 1 3H ;, d, 6. 9 Hz), 2. 07 B (3X s), 2. 58 (1H, dd, 13. 8 Hz, 10. 8 Hz), 2. 95 (lu dd, 13. 8 Hz, 5. 5 Hz), 3. 18 (1H, m), 4. 94 (lu m), 5. 51 (2X s, br), 6. 03 (1Et s), 6. 08 (lX s), 6. 57 (1H, s), 6. 64 (1H, d, 8. 4 Hz), 7. 06 (H, s), 7. 21 1H, dd, 8. 4Ha, 2. 1H, 7. 24 (H, d, 2. 1 Hz) 170 (1-5- (4-Amino-3-methylphenyl)-7 (5-cyclopropyl-124-128 47 1, 3, 4-thiadiazol-2-yl)-8-methyl-8, 9-dihydro-7H-1, 3--504. 1° (c=0. 5, CHC) dioxolo [4, 5-h] [2, 3] benzodiazepine Mp. (°C) y'leld % Example Name Solvent of [a] D recrystall. Method iH NMR (DMSO-db) d 0. 86 (2H, m), 1. 03 (2H, m), 1. 16 (3H, d, 6. 2 Hz), 2. 07 (3H, s), 2. 23 (1H, m), 2. 57 (1H, dd, 14. 0 Hz, 10. 8 Hz), 2. 95 (1H, dd, 14. 0 Hz, 5. 5 Hz), 4. 92 (1H, m), 5. 50 (2R s), 6. 03 (IK s), 6. 08 (lu s), 6. 55 (IK s), 6. 64 (1H, d, 8. 3 Hz),. 7. 05 (lu s), 7. 19 (1H, dd, 8. 3 Hz, 2. 1 Hz), 7. 23 (H, d, 2. 1 Hz) 171 (R)-5- (4-Amino-3-methylphenyl)-7- (5-hydroxymethyi- 184-186 75 - 540. 0° 1, 3, 4-thiadiazol-2-yl)-8-methyl-8, 9-dihydro-7H-1, 3-. (c =0. 5, CHC13) (c=U. 5, (J-iU3 dioxolo [4, 5-h] [2, 3] benzodiazepine Method 1H NMR DMSOd6) d 1. 20 (3H, d, 6. 1 Hz), 2. 08 (3H, s), 2. 60 (1H, dd, 13. 9 Hz, 10. 6 Hz), A 2. 97 (1H, dd, 13. 9 Hz, 5. 3 Hz), 4. 61 (2H, d, 5. 8 Hz), 4. 95 (1H, m), 5. 52 (2H, s), 5. 81 (lu t, 5. 8 Hz), 6. 03 (IK s), 6. 08 (1if s), 6. 58 (lI-1, s), 6. 65 (1F- d, 8. 5 Hz), 7. 06 (lu s), 7. 19 (lu dd, 8. 5 Hz, 2. 0 Hz), 7. 26 (1H, d, 2. 0 Hz) 172 09-7- (5-Acetoxymethyl-1, 3, 4-thiadiazol-2-yl)-5- (4- 204-206 45 - 560. 0° amino-3-methylphenyi)-8-methyl-8, 9-diSydro-7H-1, 3- (EtOE3) 560 0° dioxolo [4, 5-h] [2, 3] benzodiazepine MethodiHNMR (DMSO-) d 1. 21 (3H, d, 6. 2 Hz), 2. 07 (3H, s), 2. 08 (3H, s), 2. 60 (1H, dd, 14. 0 Hz, E 11. 0 Hz), 2. 97 (lu dd, 14. 0 Hz, 5. 4 Hz), 4. 99 (1H, m), 5. 20 (1H, d, 13. 1 Hz), 5. 24 (1H, d, 13. 1 Hz), 5. 55 (2riz s), 6. 03 (1H, s), 6. 09 (1H, s), 6. 59 (1H, s), 6. 65 (1H, d, 8. 4 Hz), 7. 06 (1H, s), 7. 21 (1H, dd, 8. 4 Hz, 2. 1 Hz), 7. 26 (H, d, 2. 1 Hz) 173 (R)-5- (4-Amino-3-methlphenyl)-7- (5-cyanomethyl-135-140 25 - 517. 9° 1, 3, 4-thiadiazol-2-yl)-8-methyl-8, 9-dihydro-7H-1, 3-517 9° (c=0. 5, (JriCb) dioxolo [4, 5-h] [2, 3] benzodiazepine Method tH NE (DMSOd6) d 1. 21 (3H, d, 6. 2 Hz), 2. 08 (3H, s), 2. 61 (1H, dd, 13. 9 Hz, 11. 0 Hz), A 2-. 99 (lu dd, 13. 9 Hz, 5. 5 Hz), 4. 40 (2riz s), 4. 96 (1H, m), 5. 55 (2H, s), 6. 03 (1H, d, 0. 7 Hz), 6. 09 (1H, d, 0. 7 Hz), 6. 59 (1H, s), 6. 65 (lu d, 8. 3 Hz), 7. 06 (1H, s), 7. 21 (1X dd, 8. 3 Hz, 1. 8 HzA, 7. 26 (lu d, 1. 8 Hz) 174 (R)-5- (4-Amino-3-methylphenyl)-8-methyl-7- (5- 177-180 57 - 496. 0° methylthiomethyl-1, 3, 4-thiadiazol-2-yl)-8, 9-dihydro- (EtOH)'°,, (c=0. 5, CHCb) 7H-1, 3-dioxolo [4, 5-h] [2, 3] benzodiazepine Method 1H NMR (DMSO-) d 1. 21 (3H, d, 6. 1 Hz), 2. 03 (3H, s), 2. 08 (3H, s), 2. 60 (1H, dd, 13. 8 Hz, A 10. 6 Hz), 2. 97 (1H, dd, 13. 8 Hz, 5. 3 Hz), 3. 91 (1H, d, 14. 7 Hz), 3. 95 (1H, d, 14. 7 Hz), 4. 96 (1H, m), 5. 52 (2X s), 6. 03 (1H, d, 0. 9 Hz), 6. 09 (1H, d, 0. 9 Hz), 6. 59 (1H, s), 6. 65 (1I d, 8. 4 I-fi), 7. 06 (H, s), 7. 21 (H, dd, 8. 4 Hz, 2. 0 Hz), 7. 25 (1H, d, 2. 0 Hz) 175 (R)-5- (4-Amino-3-methylphenyl)-7- (5-ethoxycarbonyl- 135-140 86 - 606. 3 ° 1, 3, 4-thiadiazol-2-yl)-8-methyl-8, 9-dihydro-7H 1, 3- (c=0. 5, CHCI) dioxolo [4, 5-h] [2, 3] benzodiazepine Method iH NMR (DMSOd6) d 1. 15 (3H, d, 6. 2 Hz), 1. 30 (3H, t, 7. 1 Hz), 2. 09 (: X s), 2. 64 (1H, dd, p 13. 8 Hz, 11. 3 Hz), 2. 99 (1H, dd, 13. 8 Hz, 5. 4 Hz), 4. 30 (2riz m), 5. 11 (1H, m), 5. 65 (21-4 s), 6. 04 (1H, s), 6. 09 (1X s), 6. 61 (1H, s), 6. 65 (lu d, 8. 3 Hz), 7. 08 (1H, s), 7. 25 (1H, dd, 8. 3 Hz, 2. 0 Hz), 7. 26 (1X d 2. 0 Hz) 176 (R)-5- (4-Amino-3-methylphenyl)-7- (5-aminomethyl- 139-140 31 - 482. 2° 1, 3, 4-thiadiazol-2-yl)-8-methyi-8, 9-dihydro-7H-1, 3- (EtOI (c, 5' 3) (c=0. 5, GHGi3) dioxolo [4, 5-h] [2, 3] benzodiazepine 0 Number of., '-Yield (%) . Name Solvent of Example rec stall. [ recrystah. Method 1H NMR (I) MSAds) d 1. 21 (3H, d, 6. 0 Hz), 2. 08 (3H, s), 2. 59 (1H, dd, 13. 9 Hz, 13. 9 Hz), 2. 95 (1H, dd, 13. 9 Hz, 5. 1 Hz), 3. 87 (2H, s), 4. 95 (1H, m), 5. 50 (2X s), 6. 03 (1H, s), 6. 07 (IH, s), 6. 57 (1H, s), 6. 65 (1H, d, 8. 1 Hz), 7. 05 (1H, s), 7. 21 (1H, d, br, 8. 1 Hz), 7. 26 (1X s, br) 177 (R)-5- (4-Amino-3-methylphenyi)-8-methyl-7- {5- [1- 139-143 65 - 498. 9° (lE)-propen-1-yl} 1, 3, 4-thiadiazol-2-yl}-8, 9-dihydro- (cM 7H-1, 3-dioxolo [4, 5-h] [2, 3] benzodiazepine MethodiHNMR (DMSO-) d 1. 21 (3H, d, 6. 2 Hz), 1. 86 (1H dd, 6. 8 Hz, 1. 8 Hz), 2. 08 (3H, s), 2. 59 (1H, dd, 14. 0 Hz, 10. 8 Hz), 2. 98 (IK dd, 14. 0 Hz, 5. 4 Hz), 4. 98 (lI-L m), 5. 53 (2riz s, br), 6. 03 (1if s), 6. 08 (1H, s), 6. 32 (1H, dq, 15. 7 Hz, 6. 8 Hz), 6. 55 (1H, dq, 15. 7 Hz, 1. 8 Hz), 6. 57 lI s), 6. 44 (H, d, 8. 4 Hz), 7. 06 (H, s), 7. 22 1H, dd, 8. 4 Hz, 2. 1 Hz), 7. 26 (H, d, 2. 1 Hz) 178 (R)-5- (4-Amino-3-methylphenyl)-7- (5-hexyl-1, 3, 4- 180-181 75 - 485. 2° thiadiazol-2-yl)-8-methyl-8, 9-dihydro-7H-1, 3- (c=0. 5, CHC13) (c=0. 5, CJ'iU3) dioxolo [4, 5-h] [2, 3] benzodiazepine MethodiHNMR (DMSO-) d 0. 84 (3if t, 7. 0 Hz), 1. 20 (3H, d, 6. 1 Hz), 1. 2-1. 3 (6H, m), 1-62 (2X B m), 2. 07 (3H, s), 2. 58 (1H, dd, 13. 9 Hz, 10. 5 Hz), 2. 82 (2H, t, 7. 5 Hz), 2. 95 (1H, dd, 13. 9 Hz, 5. 4 I-lz), 4. 94 (1H ; m), 5. 49 (2riz s), 6. 03 (1H, s), 6. 08 (1HX s), 6. 57 (1riz s), 6. 64 (1HX d, 8. 4 Hz), 7. 05 (lH, s), 7. 19 (1H, dd, 8. 4 Hz, 2. 2 Hz), 7. 25 (II-L d, 2. 2 Hz) 179 (R)-5- (4-Amino-3-methylphenyl)-8-methyl- (5, 6- 175-180 61 dihydro-5-oxo-4H-1, 3, 4-thiadiazin-2-71)-8, 9-dihydro-686 0° 7H-1, 3-dioxolo [4, 5-h] [2, 3] benzodiazepine Method 1H NMR (DMSCLaN) d 1. 15 (3H, d, 6. 4 Hz), 2. 05 (3H, s), 2. 48 (1H, dd, 13. 8 Hz, 10. 4 Hz), B 2. 85 (IK dd, 13. 8 Hz, 5. 3 Hz), 3. 32 (2H, s), 4. 75 (1H, m), 5. 42 (2H, s), 6. 04 (1H, s), 6. 07 (liez s), 6. 55 (1if s), 6. 61 (IK d, 8. 4 Hz), 6. 99 (1H, s), 7. 15 (1H, dd, 8. 4 Hz, 1. 8 Hz), 7. 26 (1H, d, 1. 8 Hz), 10. 47 1H, s) 180-5-(4-Aminophenyl)-8-methyl-7- (1, 3, 4-oxadiazol-2- 187-190 78 yl)-8, 9-dihydro-7H 1, 3-dioxolo [4, 5--604. 0° (c=0. 5, CHC13) h] [2, 3] benzodiazepine Method 1HNMR (CDC13) d 1. 45 (3H, d, 6. 0 Hz), 2. 73 (1H, dd, 14. 0 Hz, 10. 5 Hz), 2-86 (lEr1 dd, 14. 0 Dz, 5. 5 Hz), 3. 99 (2H, s, br), 4. 94 (1H, m), 5. 98 (1H, d, 1. 0 Hz), 6. 02 (1H, d, 1. 0 Hz), 6. 64 (H, s), 6. 68 (2H, d, 8. 0 Hz), 6. 82 (1X s), 7. 56 (2H, d, 8. 0 Hz), 7. 99 (lK s) 181 (R)-5- (4-Amino-3 methylphenyl)-8-methyl-7- (5-140-145 85 methyl-1, 3, 4-oxadiazol-2-yl)-8, 9-dihydro-7H-1, 3--554. 8° (c=0. 5, CHCr ;) dioxolo [4, 5-h] [2, 3] benzodiazepine Method MS : EI (70ei : [M] + : 391, m/z : 266 CI : [M+H] + : 392 182 (R)-5- (4-Amino-3-chlorophenyl)-8-metha1-7- (5-methyl-98-100 92 1, 3, 4-thiadiazol-2-yl)-8, 9-dihydro-7H-1, 3-dioxolo--266. 0° (c=0. 5, CICl3) [4, 5-h] [2, 3] benzodiazepine Method 1H NMR (pMSOaM,) d 1. 18 (3H d, 6. 0 Hz), 2. 50 (3H, s), 2. 65 (1H, dd, 14. 0 Hz, 9. 9 Hz), c 2. 98 (IK dd, 14. 0 Hz, 4. 9 Hz), 4. 95 (1if m), 5. 95 (2H, s), 6. 05 (1H, s), 6. 09 (li-% s), 6. 63 (1H, s), 6. 83 (1H, d, 8. 6 Hz), 7. 07 (IK s), 7. 24 (1H, dd, 8. 6 Hz, 2. 0 Hz), 7. 44 (1H, d, 2. 0 Hz) MS : EI (70eV) : [M] + : 427/429, m/z : 412/414, 313/315, 286/288, 160 CL : [M+H] + : 428/30, W1+ : 427/429 0 Number of MP- (a C) Yield (%) Example [a] D recrystall. 183 p§-5- (4-Amino-3-chlorophenyl)-8-methyl-7- (5- 105-109 91 - 350. 0° methoxymethyl-1, 3, 4-thiadiazol-2-yl)-8, 9-dihydro-7Hr, =orn 1, 3-dioxolo [4, 5-h] [2, 3] benzodiazepine Method 1HNMR (DMSOd6) d 1. 20 (3H, d, 6. 1 Hz), 2. 67 (1H, dd, 14. 1 Hz, 10. 3 Hz), 3. 00 (1H, dd, C 14. 1 Hz, 5. 4 Hz), 3. 32 (3H, s), 4. 58 (1H, d, 13. 0 Hz), 4. 62 (1H, d, 13. 0 Hz), 5. 01 (1H, m), 5. 98 (2riz s, br), 6. 05 (1if s), 6. 09 (1H, s), 6. 64 (1H, s), 6. 84 (1H, d, 8. 4 Hz), 7. 07 (1H, s), 7. 29 (IK dd, 8. 4 Hz, 1. 8 Hz), 7. 44 (1H, d, 1. 8 Hz) MS : EI (70eV) : M+ : 457/459, m/z : 442/444, 313/315, 286/288, 160 CI : [M+H1+ : 458/60, [M] +. : 457/459 184 (R)-5- (4-Amino-2-bromo-3-methyiphenyl)-8-methyl-7- foam 98 (5-methyl-1, 3, 4-thiadiazol-2-yl)-8, 9-dihydro-7H-1, 3- dioxolo [4, 5-h] [2, 3] benzodiazepine Method 1HNE (CDCl3) d 1. 25 (3X d, 6. 2 Hz), 2. 25 (3X s), 2. 53 (3H, s), 2. 93 (1H, dd, 14. 6 Hz, 7. 3 Dz), 3. 25 (1H, dd, 14. 6 Hz, 3. 1 Hz), 3. 4-4. 3 (2H), 5. 43 (1H, m), 5. 92 (1H, d, 1. 4 Hz), 5. 93 (1H, d, 1. 4 Hz), 6. 36 (1H, s), 6. 69 (1if d, 8. 1 Hz), 6. 72 (lu s), 7. 12 (1H, d, 8. 1 Hz) MS : EI (70eV) : M+ : 485/487, nzlz : 470/472, 406, 265, 219 CI : [M+H] + : 486/488 185 ()-5- (4-Aminophenyl)-8-methyl-7- (3-methyl-isoxazol- 100-103 87 5-yl)-8, 9-dihydro-7H-1, 3-dioxolo- [4, 5-h] [2, 3] benzodiazepine Method 1HNMR PNBO-d6) d 1. 16 (3H, d, 6. 5 Hz), 2. 07 (3H, s), 2. 45 (1H, dd, 14. 0 Hz, 11. 5 Hz), C 2. 88 (1H, dd, 14. 0 Hz, 6. 0 Hz), 4. 58 (IK m), 5. 26 (IK s), 5. 70 (2H, s), 6. 02 (IK s), 6. 07 (1H, s), 6. 56 (lX s), 6. 58 (2H, d, 8. 5 Hz), 7. 03 (1H, s), 7. 36 (2H, d, 8. 5 Hz) MS : EI (70eV) : M+ : 376, mlz : 306, 265, 252, 82, 54 CI : M+El+ : 377, [1W+ : 376 186 (R)-5- (4-Aminophenyl)-8-methyl-7- (1, 2, 3-thiadiazol-5-220-221 33 yl)-8, 9-dihydro 7H-1, 3-dioxolo-705 0° (c=0. 5, CHCl3) [4, 5-h] [2, 3] benzodiazepine Method 1HNE tDMSad6) d 1. 18 (3H, d, 6. 1 Hz), 2. 60 (1H, dd, 13. 8 Hz, 11. 4 Hz), 2. 95 (1H, dd, A 13. 8 Hz, 5. 1 Hz), 4. 75 (1H, m), 5. 81 (2riz s, br), 6. 03 (1H, s), 6. 08 (1H, s), 6. 59 (1H, s), 6. 61 (2H, d, 8. 4 Hz), 7. 08 (1H, s), 7. 35 (2H, d, 8. 4 Hz), 8. 10 (1H, s) MS : EI (70eV) : D/U+ : 379, nit : 351, 336, 279, 252 CI : [M+H] + : 380, mlz : 352 187 ()-5- (4-Aminophenyl)-8-methyl-7- (2-methyl-1, 3- 148-150 88 oxazol-5-yl)-8, 9-dihydro-7H-1, 3-dioxolo- [4, 5-h] [2, 3] benzodiazepine Method lHNMR (DMOa ;) d 1. 07 (3H, d, 5. 3 Hz), 2. 23 (3H, br), 2. 40 (1H, dd, 13. 7 Hz, 7. 7 Hz), A 2. 83 (1g dd, 13. 7 Hz, 5. 6 Hz), 4. 31 (1H, m), 5. 58 (s, br), 6. 03 (IK s), 6. 05 (IK s), 6. 54 (2H, d, 8. 2 Hz), 6. 57 (1H, s), 7. 00 (1H, s), 7. 28 (2R d, 8. 2 Hz) MS : EI (70eV) : M+ : 376, au : 335, 306, 265, 252 CI : [M+H] + : 377 188 ()-5- (4-Aminophenyl)-8-methyl-7- (2, 4-dimethyl-1, 3- 180-182 92 oxazol-5-yl)-8, 9-dihydro-7H-1, 3-dioxolo- [4, 5-h] [2, 3] benzodiazepine O Number of P' Yield (%) Example Name Solvent of [a] D rys Method 1H NMR (DMSO-ds) d 0. 94 (3H, d, 6. 1 Hz), 1. 76 (3X s), 2. 29 (3H, s), 2. 43 (1H, dd, 13. 8 A Hz, 3. 3 Hz), 2J9 (1X dd, 13. 8 Hz, 6. 6 Hz), 4. 13 (1if m), 5. 53 (s, br), 6. 07 (IK s), 6. 08 (lu s), 6. 52 (2H, d, 8. 4 Hz), 6. 58 (1H, s), 6. 99 (lu s), 7. 18 (2H, d, 8. 4 Hz) MS : EI (70eV) : [Mj : 390, nVz : 349, 334, 306, 279, 265, 252 CI : [M+H] + : 391, D/U+ : 390 189 (R)-5- (4-Amino-3, 5-dimethylphenyl)-8-methyl-7-202-203 75 - 686. 0° (thiazol-2-yl)-8, 9-dihydro-7H-1, 3-dioxolo- (c=0. 3, CHC : L3) [4, 5-h] [2, 3] benzodiazepine Method MS : EI (70eV) : [M] + : 406, m/z : 391, 307, 280 A 190 (19-5- (4-Amino-3, 5-dimethylphenyl)-8-methyl-7- (5- 280-281 74 methyl-1, 3, 4-thiadiazol-2-yl)-8, 9-dihydro-7H-1, 3- (c =0. 5, GHCI3) (c=0. 5, CHC13) dioxolo [4, 5-h] [2, 3] benzodiazepine Method 1H NMR (DMSOaN) d 1. 18 (3H, d, 6. 1 Hz), 2. 11 (6riz s), 2. 49 (3H, s), 2. 57 (1H, dd, 13. 9 A Hz, 10. 8 Hz), 2. 95 (1H, dd, 13. 9 Hz, 5. 1 Hz), 4. 93 (1H, m), 5. 19 (2H, s, br), 6. 03 (1H, d, 0. 5 Hz), 6. 08 (1H, d, 0. 5 Hz), 6. 56 (IK s), 7. 05 (lI- s), 7. 12 (2H, s) MS : EIpOeV) : M+ : 421, nVz : 406, 307, 306, 280 CI : [M+H] + : 422 191 (R)-5- (4-Amino-3, 5-dimethylphenyl)-8-methyl-7- (5- 148-150 87 methyl-1, 3, 4-oxadiazol-2-yl)-8, 9-dihydro-7H-1, 3-705. 0° (c=0. 5, CHQ) dioxolo [4, 5-h] [2, 3] benzodiazepine Method MS : EI (70eV) : D/U+ : 405, niz : 280, 245, 134, 83, 77 A Cl : [M+E3J+ : 406 A 192-5- (4-Aminophenyl)-8-methyl-7- (2-methyl-3-oxo-185-190 60 2, 3-dihydro-1, 2, 4-thiadiazol-2-yl)-8, 9-dihydro-7H-1, 3--45. 0° (c=0. 47, CHCis) dioxolo- [4, 5-h] [2, 3] benzodiazepine Method tHNMR (DMSOd6) d 1. 19 (3X d, 6. 5 Hz), 2. 69 (1H, dd, 14. 0 Hz, 10. 0 Hz), 3. 00 (1H, dd, A 14. 0 Hz, 4. 5 Hz), 3. 05 (3H, s), 4. 89 (1H, m), 5. 81 (2H, s, br), 6. 05 (1H, s), 6. 08 (1H, s), 6. 58 (2H, d, 8. 5 dz), 6. 59 (1H, s), 7. 06 (1H, s), 7. 26 (2H, d, 8. 5 Hz) MS : EI (70e'V) : [M] +. : 409, rdz : 279, 252 Cf : [M+H] + : 410, [Mj : 409 193 ()-5- (4-Aminophenyl)-8-methyl-7- (5, 5-dimethyl-4- 124-130 75 oxo-4, 5-dihydrothiazol-2-yi)-8, 9-dihydro-7H-1, 3- amorphous dioxolo- [4, 5-h] [2, 3] benzodiazepine Method MS : EI (70e : [m+ : 422, s7XJz 407, 279, 252 Cl d : N+H] + : 423 Example 194 (R)-7-(4,5-Dihydro-thiazol-2-yl)-5-(4-chlorophenyl)-8-methyl -8,9-dihydro-7H-1,3- dioxolo 4, 5-h] [2, 3] benzodiazepine Step A (R)-7-(tert-Butoxycarbonyl)-5-(4-doloropbenyl)-8-methyl-8,9- dibydro-7H-1,3-dioxdo[4,5-b][2,3]benzodiazepine [00293] The compound was prepared according to a synthesis described in literature (Anderson etat, J. Am CEvem Soc 117: 12358 (1995)) with the exception that tert-butylcarbazate and 4-chlorobenzaldehide were used instead of acetic hydrazide and 4-nitrobenzaldehide, respectively. The title product was isolated as a foam and used for the next step.

Step B (R)-5-(4-Cbloropbenyl)-8-methyl-8,9-dibydro-7H-1,3-dioxodo[4 ,5-b][2,3]benzodiazepine [00294] 11.0 g (28.2 mmol) of the product obtained in Step A was dissolved in 120 rnl of ethyl acetate containing 10% hydrochloric acid and stirred for 3 h, then the solution was washed with sodium carbonate and water. After drying and evaporation the crude product was reciTstaHized ftoin ethyl acetate to yield 5.07 g (57%) of the title compound. Mp.: 185-187°C ; [α]D: + 241.0° (c=0.5; CHCl3).

Step C (R)-5-(4-Cbloropbenyl)-8-metbyl-7-tbiocarbamoyl-8,9-dibydro- 7H-1,3-dioxdo[4,5-b][2,3]benzodiazepine [00295] A mixture containing 1.57 g (5.0 mmol) of the product obtained in Step B, 0.73 g (7.5 mmol) of potassium thiocyanate and 16 ml of acetic acid was stirred at 110°C for 3 h. After cooling water was added and the precipitated crystals were filtered off, washed with water and dried to yield 1. 73 g (92%) of the title compound. Mp. : 208-212°C.

Step D [00296] The compound obtained in Step C (1.0 g, 2.66 mM) was reacted with 2.20 g (10.7 mmol) of 2-bromoethylamine hydrobromide in 5 ml of dimethylformamide according to the method described in Example 9. The product was isolated by column chromatography and an additional recrystallization from ethyl acetate to yield 0.26 g (25%) of the title compound. Mp.: 216-219°C ; [OCID : +326. 7° (c=0.5 ; CHCl3).

[00297] 1H NMR (DMSO-d6) # 1.11 (3H, d, 5.7 Hz), 2.79 (1H, dd, 14.7 Hz, 6.4 Hz), 3.16 (1H, dd, 14.7 Hz, 1.7 Hz), 3.25 (2H, m), 4.16 (2H, m), 4.28 (2riz m), 5. 25 (1H, m), 5.99 (2H, s), 6.59 (1H, s), 6.71 (1H, s) 7.34 (2ES, d, 8.0 Hz), 7.53 (2H, d, 8.0 Hz) Example 195 (R)-5-(4-Chlorophenyl)-8-methyl-7-(2-thiazolyl)-8,9-dihydro- 7H-1,3-dioxolo[4, 5- hl [2, 3] benzodiazepine [00298] The compound obtained in Step C of Example 194 (0.55 g, 1.47 mmol) was reacted with 0.22 ml (1.47 mmol) of bromoacetaldehyde diethyl acetal according to the method described in Example 1. The crude product was purified by column chromatography using a mixture of n-hexane-ethyl acetate (2: 1) as eluent. After concentration of the fractions containing the title compound the residue was treated with water to yield 0.40 g (68%) of the. title compound. Mp.: 116-117°C ; zozo D : +118. 6° (c=0.5 ; CHC13).

[00299] 1HNMR (CDDC13) 8 1.22 (3H, d, 6.4 Hz), 2.83 (1H, dd, 14.6 Hz, 7.2 Hz), 3.18 (1H, dd, 14.6 Hz, 3.4 Hz), 5.38 (1H, m), 6.01 (2H, s), 6.61 (1H, s), 6.69 (1H, d, 3.7 Hz), 6. 78 (1H, s), 7.32 (1H, d, 3.7 Hz), 7.38 (2H, d, 8.6 Hz), 7.58 (2riz d, 8.6 Hz) Example 196 (R)-5-(4-Chlorophenyl)-8-methyl-7-(5-methyl-1,3,4-thiadiazol -2-yl)-8,9-dihydro-7H-1, 3- dioxolo[4,5-h][2,3]benzodiazepine Step A (R)-5-(4-Cbloropbenyl)-8-metbyl-8,9-dibydro-7H-1,3-dioxdo[4, 5-b][2,3]benzodiazepine-7-carbotbioyl doloride [00300] The compound obtained in Step B of Example 194 (2.20 g, 7.0 mmol) was reacted according to the method described for starting compound XI. The crude product was purified by column chromatography using a mixture of n-hexane-ethyl acetate (4: 1) as eluent to yield 1.38 g (49%) of the title compound as a solid foam.

Step B [00301] 1.0 g (2.48 mmol) of the product obtained in Step A was used to prepare the title compound according to a method described in Example 28, Method B. The product, isolated by column chromatography, was solidified by water to give 0.42 g (52%), Mp.: 105-108°C ; [a] D : +103. 2° (c=0. 5; CHCl3).

[00302] NMR (CDCl3) # 1.25 (3H, d, 6.2 Hz), 2.61 (3H, s), 2.84 (1H, dd, 14.3 Hz, 7. 1 Hz), 3. 18 (1H, dd, 14.3 Hz, 3.6 Hz), 5.35 (1H, m), 6.02 (2H, s), 6.57 (1H, s), 6.79 (1H, s), 7.38 (2H, d, 8.2 Hz), 7.52 (2H, d, 8.2 Hz) Example 197 (R)-5- (4-Acetylamino-3-methylphenyl)-8-methyl-7-(5-methyl-1,3,4-th iadiazol-2-yl)-8, 9- dihydro-7H-13-dioxolo [4, 5-h] [2, 3] benzodmzepme [00303] The compound of Example 119 was acetylated according to the general method described for Examples 120-131. Mp.: 267-269 °C. Yield: 67%; [a] D : -121. 0° (c=0. 5 ; CHCl3).

[00304] 1H NMR (DMSO-d6) # 1.16 (3H, d, 6.1 Hz), 2.10 (3H, s), 2.25 (3H, s), 2.50 (3H, s), 2.79 (1H, dd, 14.0 Hz, 8.2 Hz), 3.09 (1H, dd, 14.0 Hz, 4.0 Hz), 5.08 (1H, m), 6.07 (1H, s), 6.09 (1H, s), 6.55 (1H, s), 7.07 (1H, s), 7.31 (1H, d, 8.3 Hz), 7.38 (1H, s, br), 7.59 (1H, d, br, 8.3 Hz), 9.36 (1H, s) Example 198 (R)-1-Methyl-3{2-methyl-4-[8-methyl-7-(5-methyl-1,3,4-thiadi azol-2-yl)-8,9-dihydro-7H- 1,3-dioxolo[4,5-h][2,3]benzodiazepin-5-yl]}phenylurea [00305] The substance (1.03 g, 2.53 mmol) obtained in Example 119 was reacted with 0.75 ml (12.6 mmol) of methyl isocyanate in 20 ml of dichloromethane for 6 days at RT.

Evaporation of the solvent gave a crude product which was purified by column chromatography, using a mixture of hexane-ethyl acetate (2: 1) as eluent to give 0.67 g (57%) of the title compound. Mp.: 237-242 °C ; [a] D : -140. 0° (c=0.5 ; CHC13).

[00306] 1H NMR (DMSO-d6) # 1.17 (3H, d, 6.4 Hz), 2.21 (3H, s), 2.51 (3H, s), 2.66 (3H, d, 4. 6 Hz), 2.71 (1H, dd, 14. 2 Hz, 9. 4 Hz), 3.04 (lH, dd, 14. 2 Hz, 4. 5 Hz), 5.02 (lH, m), 6.05 (1H, d, 0.9 Hz), 6.08 (11-L d, 0.9 Hz), 6.55 (1H, s), 6.56 (1H, q, 4.6 Hz), 7.07 (1H, s), 7. 30 (1H, dd, 8.4 Hz, 2.0 Hz), 7.34 (1H, d, 2.0 Hz), 7.82 (1H, s), 8.00 (1H, d, 8.4 Hz) Example 199 (R)-2-Methyl-4-[8-methyl-7-(5-methyl-1,3,4-thiadiazol-2-yl)- 8,9-dihydro-7H-1,3- dioxolo[4, 5-h] [2, 3]benzodiazepin-5-yl]-phenylcarbamic acid ethyl ester [00307] The substance (0.90 g, 2.21 mmol) obtained in Example 119 was reacted with ethyl chloroformate (0.30 ml, 3.15 mmol) in dichloromethane in the presence of 0.40 ml (2.88 mmol) of triethylamine for 6 h at RT. The solution was washed with diluted hydrochloric acid and sodium hydrogen carbonate solution, dried and evaporated to dryness. The residue was purified by column chromatography using a mixture of hexane-ethyl acetate (2: 1) as eluent to give 0.45 g (42%) of the title product. Mp.: 241-244 °C ; [a] D : -180. 0° (c=0.5 ; CHC13).

[00308] 1H NMR (DMSO-d6) # 1.15 (3H, d, 6.1 Hz), 1.25 (3H, t, 7. 0 Hz), 2.25 (3H, s), 2.51 (3H, s), 2.78 (1H, dd, 14.3 Hz, 8.7 Hz), 3.05 (1H, dd, 14.3 Hz, 4.3 Hz), 4.13 (2H, q, 7.0 Hz), 5.07 (1H, m), 6.06 (1H, d, 0.7 Hz), 6.08 (1H, d, 0.7 Hz), 6.55 (1H, s), 7.07 (1H, s), 7.31 (1H, dd, 8.3 Hz, 2.0 Hz), 7.36 (1H, d, 2.0 Hz), 7.53 (1H, d, 8.3 Hz), 8.96 (1H, s) [00309] Further results with compounds of the present invention are collected in the following tables, exemplifying the AMPA antagonistic activity of compounds of formula (I).

(The corresponding in vitro and in viro investigational methods and related references were described and cited earlier in this application.) Table 13. (Supplement to Table 1) Inhibition of the"spreading deptession"in chicken retina Compound (Number of example) / IC50 µM 119 165 166 167 168 182 0.069 0.113 0. 201 0. 064 0.082 0. 020 Table 14. (Supplement to Table 2) <BR> Inhibition of ion-currents caused by 5 RM AMPA determined by the whole cell patch<BR> clamp method Compound (Number of example)/IQo ELM 119 165 166 167 168 182 0.026 0. 024 0. 028 0. 070 0. 011 0. 031 Table 15. (Supplement to Table 3) Investigation of the anticonvulsive activity in mice Compound (Number of example)/EDso mg/kg po. Method 119 165 166 167 168 182 MES 60' 2. 87 3.99 4.01 5. 85 4.49 6.17 MES 30'2. 15 2.39 4.54 4.78 2. 21 5. 08 Pentetrazol 5.00 10.10 9. 18 9.66 6.90 8.37 Strychnine 8.40 10.20 7.29 10.50 10.00 5.90 Bemegride 5. 70 10. 00 7.77 8.33 6.70 7.94 Bicuculline 2. 50 5. 79 13. 30 12. 70 10. 60 8.44 Nicotine 6. 30 18.60 21.80 10. 80 17. 70 24. 20 4-AP 2. 68 8. 60 6.81 10.80 4.60 5. 44 3-MPA 3.37 8. 53 9.92 10.30 4. 62 7. 57 Abbreviations : MES = maximal electroshock seizure; 4-AP = 4-aminopyridine ; 3-MPA = 3-mercapto-propionic acid <BR> <BR> Table 16. (Supplement to Table 4)<BR> Muscle relaxant activity in mice Compound Inclined screen Rotarod (Number of example) ED50 ip. (mg/kg) ED50 ip. (mg/kg) 119 2.49 0. 51 165 2.94 0.91 166 3.27 0.86 167 4.24 0.80 168 3. 58 0.80 182 5. 84 2.61 Table 17. (Supplement to Table 5) Inhibition of focal ischemia in rats Dose Decrease of the infracted area in % compared to Umpound,,., r<, (Number of n-ig/kg iv. that of the control example) of cver'30 min 120 min 180 nvn 240 mm example) \ 30 mm ; Time of first treatment after occlusion 119 0. 5 7 1. 0 38 1. 5 51 °'22 2. 0 _ 56 ¢ 44) 21 p p<0.05; ** p. <0.01 ; calculated with Dumett test following ANOVA (Dunnett J. Amer.

Statist Ass. 50: 1096 (1955)) [00310] Compounds of the invention were further investigated in the autoimmune encephalomyelitis model in rats as outlined in this description before, with the variance that 10 animals were used in each group, of weights-140-160 g (Lewis rats, female). The results are shown in Tables 19 and 20.

Table 18. (Supplement to Table 6) Effect of 2, 3-benzodiazepines possessing AMPA antagonist activity on the clinical symptoms of autoimmune encephalomyelitis in Lewis rats Compound Neurological symptoms (Number of Dose (change compared to controls, %) example) Female rats mg/kg i. p. mg/kg p. o. 0-8 day 0-14 day 119 3.75 -97*** -90** 1. 875-72"-71 1. 0 -75** -72** 0. 5-33-35 0. 2-36-37 3. 75 -64*** -61*** 1. 75 1. 0 -20 -23 0.5 +2 -1 166 7.5 -51* -53* 3. 75-6-9 168 7.5 -55* -56* 3.75-16-23 For statistics see Table 20.

Table 19. (Supplement to lement to Table 7) Effect of 2, 3-benzodiazepine derivatives possessing AMPA antagonistic character on the histological and clinical symptoms of autoimmune encephalomvelitis in Lewis rats on day 24 after immunization. Compound Histopathological Neurological (Number of Dose symptoms symptoms example) (change %) (change %) mg/kg i. p. mg/kg p. o. Female rats 119 3.75 +3 -90** 1. 875 -8 -71** 1. 0 -3 -72** 0.5-13-35 0.2 +2-37 3.75 -32 -61*** 1. 875 -42 -50** 1. 0 -21 -23 0.5-16-1 * p<0.05; ** p<0.01; *** p<0. 001 (Mann-Whitneytest).

Table 20. (Supplement to Table 8t<BR> <BR> Effectof2, 3-benzodiazepine derivatives possessing AMPA antagonistic character on the tremor of CD1 mice induced by different chemical agents. Compound ED50 (mg/kg po) (Number of Oxotremorin GYKI 20039 example) 1 mg/kg ip. 10 mg/kg ip. 119 1.38 (0.80-2. 38) 2.21 (1. 37-3.56) 165 4.63 (3.66-5. 85) 5.34 (3.90-7. 31) 166 2.74 (1. 97-3.00) 4.54 (3.69-5. 58) 167 4.81 (3.45-6. 72) 7.73 (4.99-11. 98) 168 3.29 (2.63-4. 12) 4.11 (3.22-5. 45) 182 2.66 (1. 24-5.72) 3.64 (2.37-5. 57) Table 21. (Supplement to Table 9)<BR> <BR> Effect of the compound described in Example 119 on the bronchial hvpersensitivity and the eosinophilia of the airways of BN-rats sensitized with ovalbumin and antigen challenged by inhalation (mean+SE N=10, p determined by Student's t-test). Compound (Number of example) Parameter Control Challenge 119 3.0 mg/kg po ED50* 5.19~0. 07 5. 970. 29 4. 350. 36 p 0.001 0.001 1000 15421 827 p O. OOZ 0. 009 Eosinophil*** 0.15~0. 03 1. 170. 18 1. 160. 24 p 0.001 NS# acetylcholine (Ach) concentration (-log M) which causes a 50% contraction compared to the control relative contraction compared to the control at a maximal Ach concentration BALF eosinope number (xlO6/ml) not significant (p >0. 05) Equivalents [00311] While the claimed invention has been described in detail and with reference to specific embodiments thereof, it will be apparent to one of ordinary skill in the art that various changes and modifications can be made to the claimed invention without departing from the spirit and scope thereof. Thus, for example, those skilled in the art will recognize, or be able to ascertain, using no more than routine experimentation, numerous equivalents to the specific substances and procedures described herein. Such equivalents are considered to be within the scope of this invention, and are covered by the following claims.