Title:
NOVEL USE AS TAG
Document Type and Number:
WIPO Patent Application WO/2007/000972
Kind Code:
A1
Abstract:
Disclosed is a novel tag which is used for the modification of a target protein. Also disclosed is a means for capturing the target protein using the tag. Specifically, study is made on a novel tag for a protein based on the knowledge about such a molecular mechanism that a DNA-binding protein can bind to DNA. As a result, it is found that when a DNA-bindable amino acid sequence of a DNA-binding protein, particularly a DNA-binding transcription factor or DNA-binding nuclear receptor is used as a tag for a target protein, a synthesized fusion protein of the tag and the target protein can be captured readily, whereby the target protein can be purified, detected or labeled. This finding leads to the accomplishment of the invention.
Inventors:
ENDO YAETA (JP)
SAWASAKI TATSUYA (JP)
KAMURA NAMI (JP)
MATSUBARA YUKO (JP)
SAWASAKI TATSUYA (JP)
KAMURA NAMI (JP)
MATSUBARA YUKO (JP)
Application Number:
PCT/JP2006/312715
Publication Date:
January 04, 2007
Filing Date:
June 26, 2006
Export Citation:
Assignee:
CELLFREE SCIENCES CO LTD (JP)
ENDO YAETA (JP)
SAWASAKI TATSUYA (JP)
KAMURA NAMI (JP)
MATSUBARA YUKO (JP)
ENDO YAETA (JP)
SAWASAKI TATSUYA (JP)
KAMURA NAMI (JP)
MATSUBARA YUKO (JP)
International Classes:
C12N15/09; C07K1/22; C07K17/00; C12Q1/00
Other References:
NILSSON J. ET AL.: "Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins", PROTEIN EXPRESSION AND PURIFICATION, vol. 11, 1997, pages 1 - 16, XP002247084
JARRETT H.W. ET AL.: "Affinity chromatography with nucleic acid polymers", J. CHROMATOGRAPHY, vol. 618, 1993, pages 315 - 339, XP002000129
CHATTOPADHYAY S. ET AL.: "Arabidopsis bZIP protein HY5 directly interacts with light-responsive promoters in mediating light control of gene expression", PLANT CELL, vol. 10, 1998, pages 673 - 683, XP003003797
FAYARD E. ET AL.: "LRH-1: an orphan nuclear receptor involved in development, metabolism and steroidogenesis", TRENDS IN CELL BIOLOGY, vol. 14, no. 5, 2004, pages 250 - 260, XP002292838
JARRETT H.W. ET AL.: "Affinity chromatography with nucleic acid polymers", J. CHROMATOGRAPHY, vol. 618, 1993, pages 315 - 339, XP002000129
CHATTOPADHYAY S. ET AL.: "Arabidopsis bZIP protein HY5 directly interacts with light-responsive promoters in mediating light control of gene expression", PLANT CELL, vol. 10, 1998, pages 673 - 683, XP003003797
FAYARD E. ET AL.: "LRH-1: an orphan nuclear receptor involved in development, metabolism and steroidogenesis", TRENDS IN CELL BIOLOGY, vol. 14, no. 5, 2004, pages 250 - 260, XP002292838
Attorney, Agent or Firm:
SHOJI, Takashi (2-10 Iwamotocho 3-chom, Chiyoda-ku Tokyo 32, JP)
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