Title:
A NOVEL WASH BUFFER SOLUTION FOR AFFINITY CHROMATOGRAPHY
Document Type and Number:
WIPO Patent Application WO/2021/147857
Kind Code:
A1
Abstract:
Provided is a method for improving impurities removal in the protein purification by affinity chromatography, comprising 1) loading a protein sample onto an affinity chromatography column, 2) washing the column with a wash buffer solution comprising Histidine or Imidazole, and a pH-adjusting agent.
Inventors:
LIU HAIKUAN (CN)
PANG QIAN (CN)
WU JING (CN)
PANG QIAN (CN)
WU JING (CN)
Application Number:
PCT/CN2021/072701
Publication Date:
July 29, 2021
Filing Date:
January 19, 2021
Export Citation:
Assignee:
WUXI BIOLOGICS SHANGHAI CO LTD (CN)
WUXI BIOLOGICS IRELAND LTD (IE)
WUXI BIOLOGICS IRELAND LTD (IE)
International Classes:
C07K1/16
Foreign References:
| CN105669844A | 2016-06-15 | |||
| CN106046149A | 2016-10-26 | |||
| CN107475215A | 2017-12-15 | |||
| CN108977456A | 2018-12-11 | |||
| CN106349387A | 2017-01-25 | |||
| CN106496321A | 2017-03-15 | |||
| CN108611358A | 2018-10-02 | |||
| CN109022472A | 2018-12-18 | |||
| CN109929032A | 2019-06-25 | |||
| CN110468172A | 2019-11-19 | |||
| CN1473843A | 2004-02-11 |
Other References:
WINGFIELD PAUL T.: "Overview of the Purification of Recombinant Proteins", CURRENT PROTOCOLS IN PROTEIN SCIENCE, no. Suppl. 80, 1 April 2015 (2015-04-01), US , pages 6.1.1 - 6.1.35, XP055832943, ISSN: 1934-3655, DOI: 10.1002/0471140864.ps0601s80
See also references of EP 4093745A4
See also references of EP 4093745A4
Attorney, Agent or Firm:
ANJIE LAW FIRM et al. (CN)
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