Title:
NUCLEAR REPROGRAMMING METHOD
Document Type and Number:
WIPO Patent Application WO/2009/057831
Kind Code:
A1
Abstract:
Disclosed is a method for producing a safe artificial pluripotent stem cell from
a somatic cell efficiently. The method comprises the step of introducing the
following three genes: an Oct family gene, a Klf family gene and a Sox family gene
into the somatic cell, or the step of introducing the combination of the following
two genes: an Oct family gene and a Sox family gene or the combination of the following
two genes: an Oct family gene and a Klf family gene and at least one gene selected
from the following three genes: an L-Myc gene, a Sall1 gene and a Sall4 gene into
the somatic cell.
Inventors:
YAMANAKA SHINYA (JP)
TAKAHASHI KAZUTOSHI (JP)
NAKAGAWA MASATO (JP)
TAKAHASHI KAZUTOSHI (JP)
NAKAGAWA MASATO (JP)
Application Number:
PCT/JP2008/070365
Publication Date:
May 07, 2009
Filing Date:
October 31, 2008
Export Citation:
Assignee:
UNIV KYOTO (JP)
International Classes:
C12N15/09; C12N5/074; C12N5/10
Domestic Patent References:
| WO2007069666A1 | 2007-06-21 | |||
| WO2008118820A2 | 2008-10-02 |
Foreign References:
| CN101250502A | 2008-08-27 |
Other References:
TAKAHASHI K ET AL.: "Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.", CELL, vol. 126, no. 4, 2006, pages 663 - 676, XP003013968
TAKAHASHI K ET AL.: "Induction of pluripotent stem cells from adult human fibroblasts by defined factors.", CELL, vol. 131, no. 5, 30 November 2007 (2007-11-30), pages 861 - 872, XP002530547
YU J. ET AL.: "Induced pluripotent stem cell lines derived from human somatic cells.", SCIENCE, vol. 318, no. 5858, 21 December 2007 (2007-12-21), pages 1917 - 1920, XP009105055
NAKAGAWA M. ET AL.: "Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts.", NAT BIOTECHNOL., vol. 26, no. 1, January 2008 (2008-01-01), pages 101 - 106, XP009098334
WU Q ET AL.: "Sall4 interacts with Nanog and co-occupies Nanog genomic sites in embryonic stem cells.", J BIOL CHEM., vol. 281, no. 34, 2006, pages 24090 - 24094, XP008109817
TAKAHASHI K ET AL.: "Induction of pluripotent stem cells from adult human fibroblasts by defined factors.", CELL, vol. 131, no. 5, 30 November 2007 (2007-11-30), pages 861 - 872, XP002530547
YU J. ET AL.: "Induced pluripotent stem cell lines derived from human somatic cells.", SCIENCE, vol. 318, no. 5858, 21 December 2007 (2007-12-21), pages 1917 - 1920, XP009105055
NAKAGAWA M. ET AL.: "Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts.", NAT BIOTECHNOL., vol. 26, no. 1, January 2008 (2008-01-01), pages 101 - 106, XP009098334
WU Q ET AL.: "Sall4 interacts with Nanog and co-occupies Nanog genomic sites in embryonic stem cells.", J BIOL CHEM., vol. 281, no. 34, 2006, pages 24090 - 24094, XP008109817
Attorney, Agent or Firm:
TANAKA, Mitsuo (IMP Building3-7, Shiromi 1-chome, Chuo-k, Osaka-shi Osaka 01, JP)
Download PDF:
Previous Patent: WELDED STATE MONITORING DEVICE AND METHOD
Next Patent: METHOD OF MANUFACTURING GLASSES FRAME MADE OF POLYETHERIMIDE RESIN
Next Patent: METHOD OF MANUFACTURING GLASSES FRAME MADE OF POLYETHERIMIDE RESIN