H. pylori colonizes the human gastric mucosa, establishing an infection that usually persists for decades. Infection by H. pylori is prevalent worldwide. Developed countries have infection rates over 50% of the adult population, while developing countries have infection rates reaching 90% of the adults over the age of 20. (Hopkins R. J. and J. G. Morris (1994) Am. J. Med 97: 265-277).

The bacterial factors necessary for colonization of the gastric environment, and for virulence of this pathogen, are poorly understood. Examples of the putative virulence factors include the following: urease, an enzyme that may play a role in neutralizing gastric acid pH (Eaton et al., (1991) Infect. Immunol. 59: 2470-2475; Ferrero, R.L. and A. Lee (1991) Microb. Ecol. High. Dis. 4: 121-134; Labigne et al., (1991) J. Bacteriol. 173:1920-1931); the bacterial flagellar proteins responsible for motility across the mucous layer. (Hazell et al., (1986) J. Inf. Dis. 153: 658-663; Leying et al., (1992) Mol. Microbiol. 6: 2863-2874; and Haas et al., (1993) Mol. Microbiol. 8: 753-760); Vac A, a bacterial toxin that induces the formation of intracellular vacuoles in epithelial cells (Schmitt, W. and R. Haas, (1994) Molecular Microbiol. 12(2): 307-319); and several gastric tissue-specific adhesins. (Boren et al., (1993) Science 262: 1892- 1895; Evans et al., (1993) J. Bacteriol. 175: 674-683; and Falk et al., (1993) Proc. Natl.

Acad. Sci. USA 90: 2035-203).

Numerous therapeutic agents are currently available that eradicate H. pylori infections in vitro. (Huesca et. al., (1993) Zbl. Bakt. 280: 244-252; Hopkins, R. J. and J.

G. Morris, supra). However. many of these treatments are suboptimally effective in vivo because of bacterial resistance, altered drug distribution, patient non-compliance or poor drug availabilty. (Hopkins, R. J. and J. G. Morris, supra). Treatment with antibiotics combined with bismuth are part of the standard regime used to treat H. pylori infection.

(Malfertheiner. P. and J. E. Dominguez-Munoz (1993) Clinical Therapeutics 15 Supp.

B: 37-48). Recently, combinations of a proton pump inhibitors and a single antibiotic have been shown to ameliorate duodenal ulcer disease. (Malfertheiner, P. and J. E.

Dominguez-Munoz supra). However, methods employing antibiotic agents can have the problem of the emergence of bacterial strains which are resistant to these agents.

(Hopkins, R. J. and J. G. Morris, supra). These limitations demonstrate that new more effective methods are needed to combat H. pylori infections in vivo. In particular, the design of new vaccines that may prevent infection by this bacterium is highly desirable.

Summary of the Invention This invention relates to novel genes, e.g., genes encoding polypeptides such as bacterial surface proteins, from the organism Helicobacter pylori (H. pylori), and other related genes, their products, and uses thereof. The nucleic acids and peptides of the present invention have utility for diagnostic and therapeutics for H. pylori and other Helicobacter species. They can also be used to detect the presence of H pylori and other Helicobacter species in a sample; and for use in screening compounds for the ability to interfere with the H. pylori life cycle or to inhibit H. pylori infection. More specifically, this invention features compositions of nucleic acids corresponding to entire coding sequences of H pylori proteins, including surface or secreted proteins or parts thereof, nucleic acids capable of binding mRNA from H. pylori proteins to block protein translation, and methods for producing H. pylori proteins or parts thereof using peptide synthesis and recombinant DNA techniques. This invention also features antibodies and nucleic acids useful as probes to detect H. pylori infection. In addition, vaccine compositions and methods for the protection or treatment of infection by H.

pylori are within the scope of this invention.

Detailed Description of the Drawings Figure 1 is a bar graph that depicts the antibody titer in serum of mice following immunization with specific H. pylori antigens.

Figure 2 is a bar graph that depicts the antibody titer in mucous of mice following immunization with specific H. pylori antigens.

Figure 3 is a bar graph that depicts therapeutic immunization of H pylori infected mice with specific antigens dissolved in HEPES buffer.

Figure 4 is a bar graph that depicts therapeutic immunization of H. pylori infected mice with specific antigens dissolved in buffer containing DOC.

Figure 5 depicts the amino acid sequence alignment in a portion of the sequence of five H. pylori proteins (depicted in the single letter amino acid code; shown N- terminal to C-terminal, left to right).

Figure 6 depicts the amino acid sequence alignment in a portion of the sequence of four H. pylori proteins (depicted in the single letter amino acid code; shown N- terminal to C-terminal, left to right).

Figure 7 depicts the amino acid sequence alignment in a portion of the sequence of two H. pylori proteins (depicted in the single letter amino acid code; shown N- terminal to C-terminal, left to right).

Figure 8 depicts the amino acid sequence alignment in a portion of the sequence of two H. pylori proteins (depicted in the single letter amino acid code; shown N- terminal to C-terminal, left to right).

Detailed Description of the Invention In one aspect, the invention features a recombinant or substantially pure preparation of H. pylori polypeptide of SEQ ID NO: 74. The invention also includes substantially pure nucleic acid encoding an H. pylori polypeptide of SEQ ID NO: 74, such nucleic acid is contained in SEQ ID NO: 1. The H. pylori polypeptide sequences of the invention described herein are contained in the Sequence Listing, and the nucleic acids encoding H. pylori polypeptides of the invention are contained in the Sequence Listing.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 75, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 2.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 76, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 3.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 77, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 4.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 78, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 5.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 79, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 6.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 80, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 7.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 81, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 8.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 82, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 9.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 83, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 10.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 84, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 11.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 85, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 12.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 86, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 13.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 87, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 14.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 88, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 15.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 89, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 16.

In another aspect, the invention features a substantially pure nucleic acid encoding an H pylori polypeptide having an amino acid sequence of SEQ ID NO: 90, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 17.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 91, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 18.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 92, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 19.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 93, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 20.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 94, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 21.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 95, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 22.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 96, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 23.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 97, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 24.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 98, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 25.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 99, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 26.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 100, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 27.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 101, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 28.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 102, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 29.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 103, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 30.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 104, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 31.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 105, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 32.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 106, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 33.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 107, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 34.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 108, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 35.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 109, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 36.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 110, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 37.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 111, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 38.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 112, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 39.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 113, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 40.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 114, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 41.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 115, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 42.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 116, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO:43.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 117, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 44.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 118, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 45.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 119, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 46.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 120, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 47.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 121, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 48.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 122, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 49.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 123, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 50.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 124, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 51.

In another aspect, the invention features a substantially pure nucleic acid encoding an H pylori polypeptide having an amino acid sequence of SEQ ID NO: 125, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 52.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 126, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 53.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 127, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 54.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 128, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 55.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 129, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 56.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 130, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 57.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 131, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 58.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 132, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 59.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 133, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 60.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 134, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 61.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 135, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 62.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 136, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 63.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 137, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 64.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 138, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 65.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 139, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 66.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 140, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 67.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 141, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 68.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 142, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 69.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 143, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 70.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 144, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 71.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 145, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 72.

In another aspect, the invention features a substantially pure nucleic acid encoding an H. pylori polypeptide having an amino acid sequence of SEQ ID NO: 146, such as a nucleic acid comprising a nucleotide sequence of SEQ ID NO: 73.

Particularly perferred is an isolated nucleic acid comprising a nucleotide sequence encoding an H. pylori cell envelope polypeptide or a fragment thereof. Such nucleic acid is selected from the group consisting of SEQ ID NO: 3, SEQ ID NO: 25, SEQ ID NO: 48, SEQ ID NO: 16, SEQ ID NO: 10, SEQ ID NO: 45, SEQ ID NO: 35, SEQ ID NO: 37, SEQ ID NO: 7, SEQ ID NO: 39, SEQ ID NO: 55, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 28, SEQ ID NO: 30, SEQ ID NO: 52, SEQ ID NO: 54, SEQ ID NO: 56, SEQ ID NO: 58, SEQ ID NO:1, SEQ ID NO: 42, SEQ ID NO: 14, SEQ ID NO: 43, SEQ ID NO: 11, SEQ ID NO: 71, SEQ ID NO: 17, SEQ ID NO: 57, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 8, and SEQ ID NO: 21.

In another embodiment, the H. pylori cell envelope polypeptide or a fragment thereof is an H. pylori inner membrane polypeptide or a fragment thereof encoded by the nucleic acid selected from the group consisting of SEQ ID NO: 3, SEQ ID NO: 25, and SEQ ID NO: 48.

In another embodiment, the H. pylori cell envelope polypeptide or a fragment thereof is an H. pylori outer membrane polypeptide or a fragment thereof encoded by the nucleic acid selected from the group consisting of SEQ ID NO: 16, SEQ ID NO: 10,

SEQ ID NO: 45, SEQ ID NO: 35, SEQ ID NO: 37, SEQ ID NO: 7, SEQ ID NO: 39, SEQ ID NO: 55, SEQ ID NO: 18, SEQ ID NO:19, SEQ ID NO: 28, SEQ ID NO: 30, SEQ ID NO: 52, SEQ ID NO: 54, SEQ ID NO: 56, SEQ ID NO: 58, SEQ ID NO: 1, SEQ ID NO: 42, SEQ ID NO: 14, SEQ ID NO: 43, SEQ ID NO:11, and SEQ ID NO: 71.

In another embodiment, the H. pylori outer membrane polypeptide or a fragment thereof is an H. pylori polypeptide having a terminal phenylalanine residue and a C- terminal tyrosine cluster or a fragment thereof encoded by the nucleic acid selected from the group consisting of SEQ ID NO:1, SEQ ID NO: 42, SEQ ID NO: 14, SEQ ID NO: 43, SEQ ID NO:11, and SEQ ID NO:71.

In yet another embodiment, the H. pylori outer membrane polypeptide or a fragment thereof is an H. pylori polypeptide having a terminal phenylalanine residue or a fragment thereof encoded by the nucleic acid selected from the group consisting of SEQ ID NO: 16, SEQ ID NO: 45, SEQ ID NO: 35, SEQ ID NO: 37, SEQ ID NO:7, SEQ ID NO: 39, SEQ ID NO: 55, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 28, SEQ ID NO: 30, SEQ ID NO:52, SEQ ID NO:54, SEQ ID NO:56, and SEQ ID NO: 58.

Particularly preferred is an isolated nucleic acid comprising a nucleotide sequence encoding an H. pylori secreted polypeptide or a fragment thereof. Such nucleic acid is selected from the group consisting of SEQ ID NO:72, SEQ ID NO:32, SEQ ID NO:51, SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:9, SEQ ID NO:13, SEQ IN NO:22, SEQ ID NO:29, SEQ ID NO:31, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:36, SEQ ID NO:38, SEQ ID NO:40, SEQ ID NO:41, SEQ ID NO:44, SEQ ID NO:46, SEQ ID NO:49, SEQ ID NO:53, SEQ ID NO:59, SEQ ID NO:61, SEQ ID NO:62, SEQ ID NO:63, SEQ ID NO:65, SEQ ID NO:66, SEQ ID NO:67, and SEQ ID NO: 68.

Particularly preferred is an isolated nucleic acid comprising a nucleotide sequence encoding an H. pylori cellular polypeptide or a fragment thereof. Such nucleic acid is selected from the group consisting of SEQ ID NO:12, SEQ ID NO: 15, SEQ ID NO:20, SEQ ID NO:23, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 47, SEQ ID NO: 50, SEQ ID NO: 60, SEQ ID NO:64, SEQ ID NO: 69, SEQ ID NO:70, and SEQ ID NO: 73.

Particularly preferred is a purified or isolated H. pylori cell envelope polypeptide or a fragment thereof, wherein the polypeptide is selected from the group consisting of SEQ ID NO: 76, SEQ ID NO:98, SEQ ID NO: 121, SEQ ID NO:89, SEQ ID NO:83, SEQ ID NO:118, SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO:80, SEQ ID NO: 112, SEQ ID NO:128, SEQ ID NO:91, SEQ ID NO:92, SEQ ID NO:101, SEQ ID

NO: 103, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 74, SEQ ID NO: 115, SEQ ID NO: 87, SEQ ID NO: 116, SEQ ID NO: 84, SEQ ID NO: 144, SEQ ID NO: 90, SEQ ID NO: 130, SEQ ID NO: 78, SEQ ID NO: 79, SEQ ID NO: 81, and SEQ ID NO: 94.

In another embodiment, the H. pylori cell envelope polypeptide or a fragment thereof is an H. pylori inner membrane polypeptide or a fragment thereof selected from the group consisting of SEQ ID NO: 76, SEQ ID NO: 98, and SEQ ID NO: 121.

In another embodiment, the H. pylori cell envelope polypeptide or a fragment thereof is an H. pylori outer membrane polypeptide or a fragment thereof selected from the group consisting of SEQ ID NO: 89, SEQ ID NO: 83, SEQ ID NO: 118, SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO: 80, SEQ ID NO: 112, SEQ ID NO: 128, SEQ ID NO: 91, SEQ ID NO: 92, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 74, SEQ ID NO: 115, SEQ ID NO: 87, SEQ ID NO: 116, SEQ ID NO: 84, SEQ ID NO: 144, SEQ ID NO: 90, and SEQ ID NO: 130.

In another embodiment, the H. pylori outer membrane polypeptide or a fragment thereof is an H. pylori polypeptide having a terminal phenylalanine residue and a C- terminal tyrosine cluster or a fragment thereof selected from the group consisting of SEQ ID NO: 74, SEQ ID NO: 115, SEQ ID NO: 87, SEQ ID NO: 116. SEQ ID NO: 84, and SEQ ID NO:144.

In another embodiment. the H. pylori outer membrane polypeptide or a fragment thereof is an H. pylori polypeptide having a terminal phenylalanine residue or a fragment thereof selected from the group consisting of SEQ ID NO: 89. SEQ ID NO: 118, SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO: 80, SEQ ID NO: 112, SEQ ID NO: 128, SEQ ID NO: 91, SEQ ID NO: 92, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131.

Particularly preferred is a purified or isolated H. pylori secreted polypeptide or a fragment thereof, wherein the polypeptide is selected from the group consisting of SEQ ID NO: 145, SEQ ID NO: 105, SEQ ID NO: 124, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 82, SEQ ID NO: 86, SEQ ID NO: 95, SEQ ID NO: 102, SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ IDNO: 114, SEQ IDNO: 117, SEQ ID NO: 119, SEQ ID NO: 122, SEQ ID NO: 126, SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO: 135, SEQ ID NO: 136, SEQ ID NO: 138, SEQ ID NO: 139, SEQ ID NO: 140, and SEQ ID NO: 141.

Particularly preferred is a purified or isolated H. pylori cellular polypeptide or a fragment thereof, wherein the polypeptide is selected from the group consisting of SEQ ID NO: 85, SEQ ID NO: 88, SEQ ID NO: 93, SEQ ID NO: 96, SEQ ID NO: 97, SEQ

ID NO: 99, SEQ ID NO: 100, SEQ ID NO: 120, SEQ ID NO: 123, SEQ ID NO: 133, SEQ ID NO: 137, SEQ ID NO: 142, SEQ ID NO: 143, and SEQ ID NO: 146.

In another aspect, the invention pertains to any individual H. pylori polypeptide member or nucleic acid encoding such a member from the above-identified groups of H pylori polypeptides.

In another aspect, the invention features nucleic acids capable of binding mRNA of H pylori. Such nucleic acid is capable of acting as antisense nucleic acid to control the translation of mRNA of H. pylori. A further aspect features a nucleic acid which is capable of binding specifically to an H. pylori nucleic acid. These nucleic acids are also referred to herein as complements and have utility as probes and as capture reagents.

In another aspect, the invention features an expression system comprising an open reading frame corresponding to H. pylori nucleic acid. The nucleic acid further comprises a control sequence compatible with an intended host. The expression system is useful for making polypeptides corresponding to H. pylori nucleic acid.

In another aspect, the invention features a cell transformed with the expression system to produce H. pylori polypeptides.

In another aspect, the invention features a method of generating antibodies against H. pylori polypeptides which are capable of binding specifically to H. pylori polypeptides. Such antibodies have utility as reagents for immunoassays to evaluate the abundance and distribution of H pylori-specific antigens.

In another aspect, the invention features a method of generating vaccines for immunizing an individual against H. pylori. The vaccination method includes: immunizing a subject with at least one H. pylori polypeptide according to the present invention, e.g., a surface or secreted polypeptide, or active portion thereof, and a pharmaceutically acceptable carrier. Such vaccines have therapeutic and/or prophylactic utilities.

In another aspect, the invention provides a method for generating a vaccine comprising a modified immunogenic H. pylori polypeptide, e.g., a surface or secreted polypeptide, or active portion thereof, and a pharmacologically acceptable carrier.

In another aspect, the invention features a method of evaluating a compound, e.g.

a polypeptide, e.g., a fragment of a host cell polypeptide, for the ability to bind an H.

pylori polypeptide. The method includes: contacting the candidate compound with an H. pylori polypeptide and determining if the compound binds or otherwise interacts with an H. pylori polypeptide. Compounds which bind H. pylori are candidates as activators or inhibitors of the bacterial life cycle. These assays can be performed in vitro or in vivo.

In another aspect, the invention features a method of evaluating a compound, e.g.

a polypeptide, e.g., a fragment of a host cell polypeptide, for the ability to bind an H.

pylori nucleic acid, e.g., DNA or RNA. The method includes: contacting the candidate compound with an H. pylori nucleic acid and determining if the compound binds or otherwise interacts with an H. pylori polypeptide. Compounds which bind H. pylori are candidates as activators or inhibitors of the bacterial life cycle. These assays can be performed in vitro or in vivo.

The invention features H. pylori polypeptides, preferably a substantially pure preparation of an H. pylori polypeptide, or a recombinant H. pylori polypeptide. In preferred embodiments: the polypeptide has biological activity; the polypeptide has an amino acid sequence at least 60%, 70%, 80%, 90%, 95%, 98%, or 99% identical or homologous to an amino acid sequence of the invention contained in the Sequence Listing, preferably it has about 65% sequence identity with an amino acid sequence of the invention contained in the Sequence Listing, and most preferably it has about 92% to about 99% sequence identity with an amino acid sequence of the invention contained in the Sequence Listing; the polypeptide has an amino acid sequence essentially the same as an amino acid sequence of the invention contained in the Sequence Listing; the polypeptide is at least 5, 10, 20, 50, 100, or 150 amino acid residues in length; the polypeptide includes at least 5, preferably at least 10, more preferably at least 20, more preferably at least 50, 100, or 150 contiguous amino acid residues of the invention contained in the Sequence Listing. In yet another preferred embodiment, the amino acid sequence which differs in sequence identity by about 7% to about 8% from the H. pylori amino acid sequences of the invention contained in the Sequence Listing is also encompassed by the invention.

In preferred embodiments: the H. pylori polypeptide is encoded by a nucleic acid of the invention contained in the Sequence Listing, or by a nucleic acid having at least 60%, 70%, 80%, 90%, 95%, 98%, or 99% homology with a nucleic acid of the invention contained in the Sequence Listing.

In a preferred embodiment, the subject H. pylori polypeptide differs in amino acid sequence at 1,2, 3, 5, 10 or more residues from a sequence of the invention contained in the Sequence Listing. The differences, however, are such that the H. pylori polypeptide exhibits an H. pylori biological activity, e.g., the H. pylori polypeptide retains a biological activity of a naturally occurring H. pylori polypeptide.

In preferred embodiments, the polypeptide includes all or a fragment of an amino acid sequence of the invention contained in the Sequence Listing; fused, in reading frame, to additional amino acid residues, preferably to residues encoded by genomic

DNA 5' or 3' to the genomic DNA which encodes a sequence of the invention contained in the Sequence Listing.

In yet other preferred embodiments, the H. pylori polypeptide is a recombinant fusion protein having a first H. pylori polypeptide portion and a second polypeptide portion, e.g., a second polypeptide portion having an amino acid sequence unrelated to H. pylori. The second polypeptide portion can be, e.g., any of glutathione-S-transferase, a DNA binding domain, or a polymerase activating domain. In preferred embodiment the fusion protein can be used in a two-hybrid assay.

Polypeptides of the invention include those which arise as a result of alternative transcription events, alternative RNA splicing events, and alternative translational and postranslational events.

The invention also encompasses an immunogenic component which includes at least one H. pylori polypeptide in an immunogenic preparation; the immunogenic component being capable of eliciting an immune response specific for the H. pylori polypeptide, e.g., a humoral response, an antibody response, or a cellular response. In preferred embodiments, the immunogenic component comprises at least one antigenic determinant from a polypeptide of the invention contained in the Sequence Listing.

In another aspect, the invention provides a substantially pure nucleic acid having a nucleotide sequence which encodes an H. pylori polypeptide. In preferred embodiments: the encoded polypeptide has biological activity; the encoded polypeptide has an amino acid sequence at least 60%, 70%, 80%, 90%, 95%, 98%, or 99% homologous to an amino acid sequence of the invention contained in the Sequence Listing; the encoded polypeptide has an amino acid sequence essentially the same as an amino acid sequence of the invention contained in the Sequence Listing; the encoded polypeptide is at least 5, 10,20, 50, 100, or 150 amino acids in length; the encoded polypeptide comprises at least 5, preferably at least 10, more preferably at least 20, more preferably at least 50, 100, or 150 contiguous amino acids of the invention contained in the Sequence Listing.

In preferred embodiments: the nucleic acid of the invention is that contained in the Sequence Listing; the nucleic acid is at least 60%, 70%, 80%, 90%, 95%, 98%, or 99% homologous with a nucleic acid sequence of the invention contained in the Sequence Listing.

In a preferred embodiment, the encoded H. pylori polypeptide differs (e.g., by amino acid substitution, addition or deletion of at least one amino acid residue) in amino acid sequence at 1, 2, 3, 5, 10 or more residues, from a sequence of the invention contained in the Sequence Listing. The differences, however, are such that: the H.

pylori encoded polypeptide exhibits a H. pylori biological activity, e.g., the encoded H.

pylori enzyme retains a biological activity of a naturally occurring H. pylori.

In preferred embodiments, the encoded polypeptide includes all or a fragment of an amino acid sequence of the invention contained in the Sequence Listing; fused, in reading frame, to additional amino acid residues, preferably to residues encoded by genomic DNA 5' or 3' to the genomic DNA which encodes a sequence of the invention contained in the Sequence Listing.

In preferred embodiments, the subject H. pylori nucleic acid will include a transcriptional regulatory sequence, e.g. at least one of a transcriptional promoter or transcriptional enhancer sequence, operably linked to the H. pylori gene sequence, e.g., to render the H. pylori gene sequence suitable for expression in a weombinant host cell.

In yet a further preferred embodiment, the nucleic acid which encodes an H.

pylori polypeptide of the invention, hybridizes under stringent conditions to a nucleic acid probe corresponding to at least 8 consecutive nucleotides of the invention contained in the Sequence Listing; more preferably to at least 12 consecutive nucleotides of the invention contained in the Sequence Listing; more preferably to at least 20 consecutive nucleotides of the invention contained in the Sequence Listing; more preferably to at least 40 consecutive nucleotides of the invention contained in the Sequence Listing.

In a preferred embodiment, the nucleic acid encodes a peptide which differs by at least one amino acid residue from the sequences of the invention contained in the Sequence Listing.

In a preferred embodiment, the nucleic acid differs by at least one nucleotide from a nucleotide sequence of the invention contained in the Sequence Listing which encodes amino acids of the invention contained in the Sequence Listing.

In another aspect, the invention encompasses: a vector including a nucleic acid which encodes an H. pylori polypeptide or an H. pylori polypeptide variant as described herein; a host cell transfected with the vector; and a method of producing a recombinant H. pylori polypeptide or H. pylori polypeptide variant; including culturing the cell, e.g., in a cell culture medium, and isolating the H. pylori or H. pylori polypeptide variant, e.g., from the cell or from the cell culture medium.

In another aspect, the invention features, a purified recombinant nucleic acid having at least 50%, 60%, 70%, 80%, 90%, 95%, 98%, or 99% homology with a sequence of the invention contained in the Sequence Listing.

The invention also provides a probe or primer which includes a substantially purified oligonucleotide. The oligonucleotide includes a region of nucleotide sequence which hybridizes under stringent conditions to at least 8 consecutive nucleotides of sense or anti sense sequence of the invention contained in the Sequence Listing, or

naturally occurring mutants thereof. In preferred embodiments, the probe or primer further includes a label group attached thereto. The label group can be, e.g., a radioisotope, a fluorescent compound, an enzyme, and/or an enzyme co-factor.

Preferably the oligonucleotide is at least 8 and less than 10, 20, 30, 50, 100, or 150 nucleotides in length.

The invention also provides an isolated H. pylori polypeptide which is encoded by a nucleic acid which hybridizes under stringent hybridization conditions to a nucleic acid contained in the Sequence Listing.

The invention further provides nucleic acids, e.g., RNA or DNA, encoding a polypeptide of the invention. This includes double stranded nucleic acids as well as coding and antisense single strands.

The H. pylori strain, from which genomic sequences have been sequenced, has been deposited in the American Type Culture Collection (ATCC &num 55679; deposited by Genome Therapeutics Corporation, 100 Beaver Street, Waltham, MA 02154) as strain HP-J99.

Included in the invention are: allelic variations; natural mutants; induced mutants; proteins encoded by DNA that hybridizes under high or low stringency conditions to a nucleic acid which encodes a polypeptide of the invention contained in the Sequence Listing (for definitions of high and low stringency see Current Protocols in Molecular Biology, John Wiley & Sons, New York, 1989, 6.3.1 - 6.3.6 and 6.4.1-6.4.10, hereby incorporated by reference); and, polypeptides specifically bound by antisera to H.

pylori polypeptides, especially by antisera to an active site or binding domain of H pylori polypeptide. The invention also includes fragments. preferably biologically active fragments. These and other polypeptides are also referred to herein as H. pylori polypeptide analogs or variants.

Putative functions have been determined for several of the H. pylori polypeptides of the invention, as shown in Table 1.

Accordingly, uses of the claimed H. pylori polypeptides based on these identified functions, as well as other functions as described herein, are also within the scope of the invention.

In addition, the present invention encompasses H. pylori polypeptides characterized as shown in Table 1 below, including: H. pylori cell envelope proteins, H.

pylori secreted proteins, and H. pylori cellular proteins. Members of these groups were identified by BLAST homology searches and by searches for secretion signal or transmembrane protein motifs. Polypeptides related by significant homology to the polypeptides of Table 1 are also considered to be classified in the manner of the homologs shown in Table 1.

TABLE 1 ORF~Name and Group nt SeqID aa SeqID A. CELL ENVELOPE A.1 Inner membrane proteins 02ge11622 23494043~fI~6 3 76 hp5p15212~13095752~c3~36 25 98 06ep30223~20173437~f1~37 48 121 A.2 Outer membrane proteins 05ee10816~14495437~f2~13 10 83 A.2.1 Terminal phe residue 06ep11509 35954752~f2 1 16 89 06ep10615~14495437~f3~47 45 118 03ae10804~14495437~c2~38 35 108 05ae30220 917200 c3~172 37 110 04cp11202~23646885~f2~26 7 80 05ep10815 16131925~c2~97 39 112 09cp61003 5860877~f2 23 55 128 09ae10512~48768~c3~67 18 91 09cp11003 5860877 f3~7 19 92 hp6e12267 30478562~f3~33 28 101 06cp30603 34174212~c3~71 30 103 09cp10224~1962590~f3~31 52 125 09cp61003 30478562~c3 106 54 127 11ae80818~10553192~f2~16 56 129 11ee11408~10584582~c3~51 58 131 A.2.2 Terminal phe residue and C- terminal tyrosine cluster 01ae12001~116018~c2 40 1 74 06ap10609 116018~c3~50 42 115 06cp30603~4687507~f1~9 14 87 06cp30603~4687507~f1~7 43 116 05ee10816~36126938~f3~16 11 84 01cp20708 4960952~c1~43 71 144 A.3 Via homolgy 07ap80601 5083193 f3~8 17 90 11ap20714 4797137 f3~45 57 130 A.4 Other cell envelope proteins 04ap12016~25501501~f1~1 5 78 04cp11202~20415937~f2~25 6 79 04ee11108~3906963~f1~7 8 81 29ep10720~25501501~c2~33 21 94 B. B SECRETED PROTEINS hp3e10342 22448587~c2~15 72 145 hp5p15212~24276587~f1~2 32 105 09ce10413~35336707~f2~9 51 124 01ae12001~32462543~c2~43 2 75 03ee11215~1416312~c3~35 4 77 05ae30220 14570443 c2 94 9 82 06cp30603 2772578 cI 46 13 86 29ep10720~289077~f2~12 22 95 03ee11215 2254803~f1~7 29 102 09ae10512 3166040 c1 40 31 104 01ce11104~10742963~c2~12 33 106 02ge10116~36335436~f3~66 34 107 04ep41903 11876461~fI 4 36 109 05ce10208~23631292~f1~6 38 111 05ep10815~22447252~c3~110 40 113 05ep10815 30283516~c3~109 41 114 06ee30709~33851038~c3~30 44 117 06ep11202~21687842~c3~35 46 119 06ep30223 2774062 f1~33 49 122 09cp10713~23912707~c1~26 53 126 11ee11408~4882318~f3~24 59 132 hp4e13394 5908553 fi 1 61 134 hp4e53394~1416312~c3~119 62 135 hp5e15211 24328910 c3 38 63 136 hp6p10606~23493756~c1~21 65 138 hp6p22217~23564012~f1~5 66 139 hp6p22217 272058~f1~2 67 140 hp6p22217~2922143~f2 9 68 141 C. OTHER CELLULAR PROTEINS 06ap11119 14726542~f3~21 12 85 06ee10709 6136430~c1~11 15 88 12ap10605~14094816~c1~5 20 93 hp2p10272~34042518~f1~2 23 96 hp5e15211~25411557~c1~22 24 97 hp5p15641~3907968~fl~3 26 99 hp6e10967 657638~f3~9 27 100 06ep11202~4569693~c2~28 47 120 06ep30223~3930468~c1~110 50 123 hp2e10911~960952~c2~86 60 133 hp6p10509~14642217~c2~17 64 137 hp6p80503 20964382~f2~11 69 142 hp7e10192~5917593~f1~2 70 143 hp6p10509 14642217~c3 25 73 146

[In Table 1, "nt" represents nucleotide Seq. ID number and "aa" represents amino acid Seq. ID number] Definitions The terms "purified polypeptide" and "isolated polypeptide" and "a substantially pure preparation of a polypeptide" are used interchangeably herein and as used herein

from other proteins, lipids, and nucleic acids with which it naturally occurs. Preferably, the polypeptide is also separated from substances, e.g., antibodies or gel matrix, e.g., polyacrylamide, which are used to purify it. Preferably, the polypeptide constitutes at least 10, 20, 50 70, 80 or 95% dry weight of the purified preparation. Preferably, the preparation contains: sufficient polypeptide to allow protein sequencing; at least 1, 10, or 100 ptg of the polypeptide; at least 1, 10, or 100 mg of the polypeptide. Furthermore, the terms "purified polypeptide" and "isolated polypeptide" and "a substantially pure preparation of a polypeptide," as used herein, refer to both a polypeptide obtained from nature or produced by recombinant DNA techniques as described herein.

For example, an "isolated" or "purified" protein or biologically active portion thereof is substantially free of cellular material or other contaminating proteins from the cell or tissue source from which the H. pylori protein is derived, or substantially free from chemical precursors or other chemicals when chemically synthesized. The language "substantially free of cellular material" includes preparations of H. pylori protein in which the protein is separated from cellular components of the cells from which it is isolated or recombinantly produced. In one embodiment, the language "substantially free of cellular material" includes preparations of H. pylori protein having less than about 30% (by dry weight) of non-H pylori protein (also referred to herein as a "contaminating protein"), more preferably less than about 20% of non-H. pylori protein, still more preferably less than about 10% of non-H. pylori protein, and most preferably less than about 5% non-H. pylori protein. When the H. pylori protein or biologically active portion thereof is recombinantly produced, it is also preferably substantially free of culture medium, i.e., culture medium represents less than about 20%, more preferably less than about 10%, and most preferably less than about 5% of the volume of the protein preparation.

The language "substantially free of chemical precursors or other chemicals" includes preparations of H pylori protein in which the protein is separated from chemical precusors or other chemicals which are involved in the synthesis of the protein.

In one embodiment, the language "substantially free of chemical precursors or other chemicals" includes preparations of H. pylori protein having less than about 30% (by dry weight) of chemical precursors or non-H. pylori chemicals, more preferably less than about 20% chemical precursors or non-H. pylori chemicals, still more preferably less than about 10% chemical precursors or non-H. pylori chemicals, and most preferably less than about 5% chemical precursors or non-H. pylori chemicals.

A purified preparation of cells refers to, in the case of plant or animal cells, an in vitro preparation of cells and not an entire intact plant or animal. In the case of cultured

cells or microbial cells, it consists of a preparation of at least 10% and more preferably 50% of the subject cells.

A purified or isolated or a substantially pure nucleic acid, e.g., a substantially pure DNA, (are terms used interchangeably herein) is a nucleic acid which is one or both of the following: not immediately contiguous with both of the coding sequences with which it is immediately contiguous (i.e., one at the 5' end and one at the 3' end) in the naturally-occurring genome of the organism from which the nucleic acid is derived; or which is substantially free of a nucleic acid with which it occurs in the organism from which the nucleic acid is derived. The term includes, for example, a recombinant DNA which is incorporated into a vector, e.g., into an autonomously replicating plasmid or virus, or into the genomic DNA of a prokaryote or eukaryote, or which exists as a separate molecule (e.g., a cDNA or a genomic DNA fragment produced by PCR or restriction endonuclease treatment) independent of other DNA sequences. Substantially pure DNA also includes a recombinant DNA which is part of a hybrid gene encoding additional H. pylori DNA sequence.

A "contig" as used herein is a nucleic acid representing a continuous stretch of genomic sequence of an organism.

An "open reading frame", also referred to herein as ORF, is a region of nucleic acid which encodes a polypeptide. This region may represent a portion of a coding sequence or a total sequence and can be determined from a stop to stop codon or from a start to stop codon.

As used herein, a "coding sequence" is a nucleic acid which is transcribed into messenger RNA and/or translated into a polypeptide when placed under the control of appropriate regulatory sequences. The boundaries of the coding sequence are determined by a translation start codon at the five prime terminus and a translation stop code at the three prime terminus. A coding sequence can include but is not limited to messenger RNA, synthetic DNA, and recombinant nucleic acid sequences.

A "complement" of a nucleic acid as used herein referes to an anti-parallel or anti sense sequence that participates in Watson-Crick base-pairing with the original sequence.

A "gene product" is a protein or structural RNA which is specifically encoded by a gene.

As used herein, the term "probe" refers to a nucleic acid, peptide or other chemical entity which specifically binds to a molecule of interest. Probes are often associated with or capable of associating with a label. A label is a chemical moiety capable of detection. Typical labels comprise dyes, radioisotopes, luminescent and chemiluminescent moieties, fluorophores, enzymes, precipitating agents, amplification

sequences, and the like. Similarly, a nucleic acid, peptide or other chemical entity which specifically binds to a molecule of interest and immobilizes such molecule is referred herein as a "capture ligand". Capture ligands are typically associated with or capable of associating with a support such as nitro-cellulose, glass, nylon membranes, beads, particles and the like. The specificity of hybridization is dependent on conditions such as the base pair composition of the nucleotides, and the temperature and salt concentration of the reaction. These conditions are readily discernable to one of ordinary skill in the art using routine experimentation.

Homologous refers to the sequence similarity or sequence identity between two polypeptides or between two nucleic acid molecules. When a position in both of the two compared sequences is occupied by the same base or amino acid monomer subunit, e.g., if a position in each of two DNA molecules is occupied by adenine, then the molecules are homologous at that position. The percent of homology between two sequences is a function of the number of matching or homologous positions shared by the two sequences divided by the number of positions compared x 100. For example, if 6 of 10 of the positions in two sequences are matched or homologous then the two sequences are 60% homologous. By way of example, the DNA sequences ATTGCC and TATGGC share 50% homology. Generally, a comparison is made when two sequences are aligned to give maximum homology.

Nucleic acids are hybridizable to each other when at least one strand of a nucleic acid can anneal to the other nucleic acid under defined stringency conditions.

Stringency of hybridization is determined by: (a) the temperature at which hybridization and/or washing is performed; and (b) the ionic strength and polarity of the hybridization and washing solutions. Hybridization requires that the two nucleic acids contain complementary sequences; depending on the stringency of hybridization, however, mismatches may be tolerated. Typically, hybridization of two sequences at high stingency (such as, for example, in a solution of 0.5X SSC, at 650 C) requires that the sequences be essentially completely homologous. Conditions of intermediate stringency (such as, for example, 2X SSC at 65 ° C) and low stringency (such as, for example 2X SSC at 55" C), require correspondingly less overall complementarity between the hybridizing sequences. (1X SSC is 0.15 M NaCl, 0.015 M Na citrate). A preferred, non-limiting example of stringent hybridization conditions are hybridization in 6X sodium chloride/sodium citrate (SSC) at about 450C, followed by one or more washes in 0.2 X SSC, 0.1% SDS at 50-650C.

The terms peptides, proteins, and polypeptides are used interchangeably herein.

As used herein, the term "surface protein" refers to all surface accessible proteins, e.g. inner and outer membrane proteins, proteins adhering to the cell wall, and secreted proteins.

A polypeptide has H. pylori biological activity if it has one, two and preferably more of the following properties: (1) if when expressed in the course of an H. pylori infection, it can promote, or mediate the attachment of H pylori to a cell; (2) it has an enzymatic activity, structural or regulatory function characteristic of an H. pylori protein; (3) the gene which encodes it can rescue a lethal mutation in an H. pylori gene; (4) or it is immunogenic in a subject. A polypeptide has biological activity if it is an antagonist, agonist, or super-agonist of a polypeptide having one of the above-listed properties.

A biologically active fragment or analog is one having an in vivo or in vitro activity which is characteristic of the H. pylori polypeptides of the invention contained in the Sequence Listing, or of other naturally occurring H. pylori polypeptides, e.g., one or more of the biological activities described herein. Especially preferred are fragments which exist in vivo, e.g., fragments which arise from post transcriptional processing or which arise from translation of alternatively spliced RNA's. Fragments include those expressed in native or endogenous cells as well as those made in expression systems, e.g., in CHO cells. Because peptides such as H. pylori polypeptides often exhibit a range of physiological properties and because such properties may be attributable to different portions of the molecule, a useful H. pylori fragment or H. pylori analog is one which exhibits a biological activity in any biological assay for H. pylori activity. Most preferably the fragment or analog possesses 10%, preferably 40%, more preferably 60%, 70%, 80% or 90% or greater of the activity of H. pylori, in any in vivo or in vitro assay.

Analogs can differ from naturally occurring H. pylori polypeptides in amino acid sequence or in ways that do not involve sequence, or both. Non-sequence modifications include changes in acetylation, methylation, phosphorylation. carboxylation, or glyeosylation. Preferred analogs include H. pylori polypeptides (or biologically active fragments thereof) whose sequences differ from the wild-type sequence by one or more conservative amino acid substitutions or by one or more non-conservative amino acid substitutions, deletions, or insertions which do not substantially diminish the biological activity of the H. pylori polypeptide. Conservative substitutions typically include the substitution of one amino acid for another with similar characteristics, e.g., substitutions within the following groups: valine, glycine; glycine, alanine; valine, isoleucine, leucine; aspartic acid, glutamic acid; asparagine, glutamine; serine, threonine; lysine, arginine; and phenylalanine, tyrosine. Other conservative substitutions can be made in view of the table below.

TABLE 2 CONSERVATIVE AMINO ACID REPLACEMENTS For Amino Acid Code Replace with any of Alanine A D-Ala, Gly, beta-Ala, L-Cys, D-Cys Arginine R D-Arg, Lys, D-Lys, homo-Arg, D-homo-Arg, Met, Ile, D-Met, D-Ile, Orn, D-Orn Asparagine N D-Asn, Asp, D-Asp, Glu, D-Glu, Gln, D-Gln Aspartic Acid D D-Asp, D-Asn, Asn, Glu, D-Glu, Gln, D-Gln Cysteine C D-Cys, S-Me-Cys, Met, D-Met, Thr, D-Thr Glutamine Q D-Gln, Asn. D-Asn, Glu, D-Glu, Asp, D-Asp Glutamic Acid E D-Glu, D-Asp, Asp, Asn, D-Asn, Gln, D-Gln Glycine G Ala, D-Ala, Pro, D-Pro, t3-Ala, Acp Isoleucine I D-Ile, Val, D-Val, Leu, D-Leu, Met, D-Met Leucine L D-Leu, Val, D-Val, Leu, D-Leu, Met, D-Met Lysine K D-Lys, Arg, D-Arg, homo-Arg, D-homo-Arg, Met, D- Met, Ile, D-Ile, Orn, D-Orn Methionine M D-Met, S-Me-Cys, Ile, D-Ile. Leu, D-Leu, Val, D-Val Phenylalanine F D-Phe, Tyr, D-Thr, L-Dopa. His, D-His, Trp, D-Trp, Trans-3,4, or 5-phenylproline, cis-3,4, or 5-phenylproline Proline P D-Pro, L-I-thioazolidine-4-carboxylic acid, D-or L-1- oxazolidine-4-carboxylic acid Serine S D-Ser, Thr, D-Thr, allo-Thr, Met, D-Met, Met(O), D-Met(O), L-Cys, D-Cys Threonine T D-Thr, Ser, D-Ser, allo-Thr, Met, D-Met, Met(O), D-Met(O), Val, D-Val Tyrosine Y D-Tyr, Phe, D-Phe, L-Dopa, His, D-His Valine V D-Val, Leu, D-Leu, Ile, D-Ile, Met, D-Met

Other analogs within the invention are those with modifications which increase peptide stability; such analogs may contain, for example, one or more non-peptide bonds (which replace the peptide bonds) in the peptide sequence. Also included are: analogs that include residues other than naturally occurring L-amino acids, e.g., D-amino acids or non-naturally occurring or synthetic amino acids, e.g., or y amino acids; and cyclic analogs.

As used herein, the term "fragment", as applied to an H. pylori analog, will ordinarily be at least about 20 residues, more typically at least about 40 residues, preferably at least about 60 residues in length. Fragments of H. pylori polypeptides can be generated by methods known to those skilled in the art. The ability of a candidate fragment to exhibit a biological activity of H pylori polypeptide can be assessed by methods known to those skilled in the art as described herein. Also included are H.

pylori polypeptides containing residues that are not required for biological activity of the peptide or that result from alternative mRNA splicing or alternative protein processing events.

An "immunogenic component" as used herein is a moiety, such as an H. pylori polypeptide, analog or fragment thereof, that is capable of eliciting a humoral and/or cellular immune response in a host animal alone or in combination with an adjuvant.

An "antigenic component" as used herein is a moiety, such as an H pylori polypeptide, analog or fragment thereof, that is capable of binding to a specific antibody with sufficiently high affinity to form a detectable antigen-antibody complex.

As used herein, the term "transgene" means a nucleic acid (encoding, e.g., one or more polypeptides), which is partly or entirely heterologous, i.e., foreign, to the transgenic animal or cell into which it is introduced, or, is homologous to an endogenous gene of the transgenic animal or cell into which it is introduced, but which is designed to be inserted, or is inserted, into the cell's genome in such a way as to alter the genome of the cell into which it is inserted (e.g., it is inserted at a location which differs from that of the natural gene or its insertion results in a knockout). A transgene can include one or more transcriptional regulatory sequences and any other nucleic acid, such as introns, that may be necessary for optimal expression of the selected nucleic acid, all operably linked to the selected nucleic acid, and may include an enhancer sequence.

As used herein, the term "transgenic cell" refers to a cell containing a transgene.

As used herein, a "transgenic animal" is any animal in which one or more, and preferably essentially all, of the cells of the animal includes a transgene. The transgene can be introduced into the cell, directly or indirectly by introduction into a precursor of the cell, by way of deliberate genetic manipulation, such as by a process of transformation of competent cells or by microinjection or by infection with a

recombinant virus. This molecule may be integrated within a chromosome, or it may be extrachromosomally replicating DNA.

The term "antibody" as used herein is intended to include fragments thereof which are specifically reactive with H. pylori polypeptides.

As used herein, the term "cell-specific promoter" means a DNA sequence that serves as a promoter, i.e., regulates expression of a selected DNA sequence operably linked to the promoter, and which effects expression of the selected DNA sequence in specific cells of a tissue. The term also covers so-called "leaky" promoters, which regulate expression of a selected DNA primarily in one tissue, but cause expression in other tissues as well.

Misexpression, as used herein, refers to a non-wild type pattern of gene expression. It includes: expression at non-wild type levels, i.e., over or under expression; a pattern of expression that differs from wild type in terms of the time or stage at which the gene is expressed, e.g., increased or decreased expression (as compared with wild type) at a predetermined developmental period or stage; a pattern of expression that differs from wild type in terms of decreased expression (as compared with wild type) in a predetermined cell type or tissue type; a pattern of expression that differs from wild type in terms of the splicing size, amino acid sequence, post- transitional modification, or biological activity of the expressed polypeptide; a pattern of expression that differs from wild type in terms of the effect of an environmental stimulus or extracellular stimulus on expression of the gene, e.g., a pattern of increased or decreased expression (as compared with wild type) in the presence of an increase or decrease in the strength of the stimulus.

As used herein, "host cells" and other such terms denoting microorganisms or higher eukaryotic cell lines cultured as unicellular entities refers to cells which can become or have been used as recipients for a recombinant vector or other transfer DNA, and include the progeny of the original cell which has been transfected. It is understood by individuals skilled in the art that the progeny of a single parental cell may not necessarily be completely identical in genomic or total DNA compliment to the original parent, due to accident or deliberate mutation.

As used herein, the term "control sequence" refers to a nucleic acid having a base sequence which is recognized by the host organism to effect the expression of encoded sequences to which they are ligated. The nature of such control sequences differs depending upon the host organism; in prokaryotes, such control sequences generally include a promoter, ribosomal binding site, terminators, and in some cases operators; in eukaryotes, generally such control sequences include promoters, terminators and in some instances, enhancers. The term control sequence is intended to include at a

minimum, all components whose presence is necessary for expression, and may also include additional components whose presence is advantageous, for example, leader sequences.

As used herein, the term "operably linked" refers to sequences joined or ligated to function in their intended manner. For example, a control sequence is operably linked to coding sequence by ligation in such a way that expression of the coding sequence is achieved under conditions compatible with the control sequence and host cell.

The metabolism of a substance, as used herein, means any aspect of the, expression, function, action, or regulation of the substance. The metabolism of a substance includes modifications, e.g., covalent or non-covalent modifications of the substance. The metabolism of a substance includes modifications, e.g., covalent or non- covalent modification, the substance induces in other substances. The metabolism of a substance also includes changes in the distribution of the substance. The metabolism of a substance includes changes the substance induces in the distribution of other substances.

A "sample" as used herein refers to a biological sample, such as, for example, tissue or fluid isloated from an individual (including without limitation plasma, serum, cerebrospinal fluid, lymph, tears, saliva and tissue sections) or from in vitro cell culture constituents, as well as samples from the environment.

The practice of the invention will employ, unless otherwise indicated, conventional techniques of chemistry, molecular biology, microbiology, recombinant DNA, and immunology, which are within the skill of the art. Such techniques are explained fully in the literature. See e.g., Sambrook, Fritsch, and Maniatis, Molecular Cloning; Laboratory Manual 2nd ed. (1989); DNA Cloning, Volumes I and II (D.N Glover ed. 1985); Oligonucleotide Synthesis (M.J. Gait ed. 1984); Nucleic Acid Hybridization (B.D. Hames & S.J. Higgins eds. 1984); the series, Methods in Enzymoloqy (Academic Press, Inc.), particularly Vol. 154 and Vol. 155 (Wu and Grossman, eds.) and PCR-A Practical Approach (McPherson, Quirke, and Taylor, eds., 1991).

I. Isolation of Nucleic Acids of H. pylori and Uses Therefor H. pylori Genomic Sequence This invention provides nucleotide sequences of the genome of H. pylori which thus comprises a DNA sequence library of H. pylori genomic DNA. The detailed description that follows provides nucleotide sequences of H pylori, and also describes how the sequences were obtained and how ORFs and protein-coding sequences were

identified. Also described are methods of using the disclosed H. pylori sequences in methods including diagnostic and therapeutic applications. Furthermore, the library can be used as a database for identification and comparison of medically important sequences in this and other strains of H pylori.

To determine the genomic sequence of H pylori, DNA was isolated from a strain of H. pylori (ATCC It 55679; deposited by Genome Therapeutics Corporation, 100 Beaver Street, Waltham, MA 02154) and mechanically sheared by nebulization to a median size of 2 kb. Following size fractionation by gel electrophoresis, the fragments were blunt-ended, ligated to adapter oligonucleotides, and cloned into each of 20 different pMPX vectors (Rice et al., abstracts of Meeting of Genome Mapping and Sequencing, Cold Spring Harbor, NY, 5/11-5/15, 1994, p. 225) to construct a series of "shotgun" subclone libraries.

DNA sequencing was achieved using multiplex sequencing procedures essentially as disclosed in Church et al., 1988, Science 240:185; U.S. Patents No.

4,942,124 and 5,149,625). DNA was extracted from pooled cultures and subjected to chemical or enzymatic sequencing. Sequencing reactions were resolved by electrophoresis, and the products were transferred and covalently bound to nylon membranes. Finally, the membranes were sequentially hybridized with a series of labelled oligonucleotides complimentary to "tag" sequences present in the different shotgun cloning vectors. In this manner, a large number of sequences could be obtained from a single set of sequencing reactions. The cloning and sequencing procedures are described in more detail in the Exemplification.

Individual sequence reads obtained in this manner were assembled using the FALCONTM program (Church et al., 1994, Automated DNA Sequencing and Analysis, J.C. Venter, ed., Academic Press) and PHRAP (P. Green, Abstracts of DOE Human Genome Program Contractor-Grantee Workshop V, Jan. 1996, p.157). The average contig length was about 3-4 kb.

A variety of approaches are used to order the contigs so as to obtain a continuous sequence representing the entire H. pylori genome. Synthetic oligonucleotides are designed that are complementary to sequences at the end of each contig. These oligonucleotides may be hybridized to libaries of H pylori genomic DNA in, for example, lambda phage vectors or plasmid vectors to identify clones that contain sequences corresponding to the junctional regions between individual contigs. Such clones are then used to isolate template DNA and the same oligonucleotides are used as primers in polymerase chain reaction (PCR) to amplify junctional fragments, the nucleotide sequence of which is then determined.

The H. pylori sequences were analyzed for the presence of open reading frames (ORFs) comprising at least 180 nucleotides. As a result of the analysis of ORFs based on stop-to-stop codon reads, it should be understood that these ORFs may not correspond to the ORF of a naturally-occurring H. pylori polypeptide. These ORFs may contain start codons which indicate the initiation of protein synthesis of a naturally- occurring H. pylori polypeptide. Such start codons within the ORFs provided herein can be identified by those of ordinary skill in the relevant art, and the resulting ORF and the encoded H. pylori polypeptide is within the scope of this invention. For example, within the ORFs a codon such as AUG or GUG (encoding methionine or valine) which is part of the initiation signal for protein synthesis can be identified and the ORF modified to correspond to a naturally-occurring H. pylori polypeptide. The predicted coding regions were defined by evaluating the coding potential of such sequences with the program GENEMAKKTM (Borodovsky and McIninch, 1993 Comp. Chem. 17:123).

Other H. pvlori Nucleic Acids The nucleic acids of this invention may be obtained directly from the DNA of the above referenced H. pylori strain by using the polymerase chain reaction (PCR). See "PCR, A Practical Approach" (McPherson, Quirke, and Taylor, eds., IRL Press, Oxford, UK, 1991) for details about the PCR. High fidelity PCR can be used to ensure a faithful DNA copy prior to expression. In addition, the authenticity of amplified products can be checked by conventional sequencing methods. Clones carrying the desired sequences described in this invention may also be obtained by screening the libraries by means of the PCR or by hybridization of synthetic oligonucleotide probes to filter lifts of the library colonies or plaques as known in the art (see, e.g., Sambrook et al., Molecular Cloning, A Laboratory Manual 2nd edition, 1989, Cold Spring Harbor Press, NY).

It is also possible to obtain nucleic acids encoding H. pylori polypeptides from a cDNA library in accordance with protocols herein described. A cDNA encoding an H.

pylori polypeptide can be obtained by isolating total mRNA from an appropriate strain.

Double stranded cDNAs can then be prepared from the total mRNA. Subsequently, the cDNAs can be inserted into a suitable plasmid or viral (e.g., bacteriophage) vector using any one of a number of known techniques. Genes encoding H. pylori polypeptides can also be cloned using established polymerase chain reaction techniques in accordance with the nucleotide sequence information provided by the invention. The nucleic acids of the invention can be DNA or RNA. Preferred nucleic acids of the invention are contained in the Sequence Listing.

The nucleic acids of the invention can also be chemically synthesized using standard techniques. Various methods of chemically synthesizing polydeoxynucleotides

are known, including solid-phase synthesis which, like peptide synthesis, has been fully automated in commercially available DNA synthesizers (See e.g., Itakura et al. U.S.

Patent No. 4,598,049; Caruthers et al. U.S. Patent No. 4,458,066; and Itakura U.S.

Patent Nos. 4,401,796 and 4,373,071, incorporated by reference herein).

Nucleic acids isolated or synthesized in accordance with features of the present invention are useful, by way of example, without limitation, as probes. primers, capture ligands anti sense genes and for developing expression systems for the synthesis of proteins and peptides corresponding to such sequences. As probes, primers, capture ligands and antisense agents, the nucleic acid normally consists of all or part (approximately twenty or more nucleotides for specificity as well as the ability to form stable hybridization products) of the nucleic acids of the invention contained in the Sequence Listing. These uses are described in further detail below.

Probes A nucleic acid isolated or synthesized in accordance with the sequence of the invention contained in the Sequence Listing can be used as a probe to specifically detect H. pylori. With the sequence information set forth in the present application, sequences of twenty or more nucleotides are identified which provide the desired inclusivity and exclusivity with respect to H. pylori, and extraneous nucleic acids likely to be encountered during hybridization conditions. More preferably, the sequence will comprise at least twenty to thirty nucleotides to convey stability to the hybridization product formed between the probe and the intended target molecules.

Sequences larger than 1000 nucleotides in length are difficult to synthesize but can be generated by recombinant DNA techniques. Individuals skilled in the art will readily recognize that the nucleic acids, for use as probes, can be provided with a label to facilitate detection of a hybridization product.

Nucleic acid isolated and synthesized in accordance with the sequence of the invention contained in the Sequence Listing can also be useful as probes to detect homologous regions (especially homologous genes) of other Helicobacter species using appropriate stringency hybridization conditions as described herein.

Capture Lizard For use as a capture ligand, the nucleic acid selected in the manner described above with respect to probes, can be readily associated with a support. The manner in which nucleic acid is associated with supports is well known. Nucleic acid having twenty or more nucleotides in a sequence of the invention contained in the Sequence Listing have utility to separate H. pylori nucleic acid from the nucleic acid of each other and other organisms. Nucleic acid having twenty or more nucleotides in a sequence of the invention contained in the Sequence Listing can also have utility to separate other

Helicobacter species from each other and from other organisms. Preferably, the sequence will comprise at least twenty nucleotides to convey stability to the hybridization product formed between the probe and the intended target molecules.

Sequences larger than 1000 nucleotides in length are difficult to synthesize but can be generated by recombinant DNA techniques.

Primers Nucleic acid isolated or synthesized in accordance with the sequences described herein have utility as primers for the amplification of H. pylori nucleic acid. These nucleic acids may also have utility as primers for the amplification of nucleic acids in other Helicobacter species. With respect to polymerase chain reaction (PCR) techniques, nucleic acid sequences of > 10-15 nucleotides of the invention contained in the Sequence Listing have utility in conjunction with suitable enzymes and reagents to create copies of H. pylori nucleic acid. More preferably, the sequence will comprise twenty or more nucleotides to convey stability to the hybridization product formed between the primer and the intended target molecules. Binding conditions of primers greater than 100 nucleotides are more difficult to control to obtain specificity. High fidelity PCR can be used to ensure a faithful DNA copy prior to expression. In addition, amplified products can be checked by conventional sequencing methods.

The copies can be used in diagnostic assays to detect specific sequences, including genes from H. pylori and/or other Helicobacter species. The copies can also be incorporated into cloning and expression vectors to generate polypeptides corresponding to the nucleic acid synthesized by PCR, as is described in greater detail herein.

Antisense Nucleic acid or nucleic acid-hybridizing derivatives isolated or synthesized in accordance with the sequences described herein have utility as anti sense agents to prevent the expression of H pylori genes. These sequences also have utility as antisense agents to prevent expression of genes of other Helicobacter species.

In one embodiment, nucleic acid or derivatives corresponding to H. pylori nucleic acids is loaded into a suitable carrier such as a liposome or bacteriophage for introduction into bacterial cells. For example, a nucleic acid having twenty or more nucleotides is capable of binding to bacteria nucleic acid or bacteria messenger RNA.

Preferably, the antisense nucleic acid is comprised of 20 or more nucleotides to provide necessary stability of a hybridization product of non-naturally occurring nucleic acid and bacterial nucleic acid and/or bacterial messenger RNA. Nucleic acid having a sequence greater than 1000 nucleotides in length is difficult to synthesize but can be generated by recombinant DNA techniques. Methods for loading antisense nucleic acid in liposomes

is known in the art as exemplified by U.S. Patent 4,241,046 issued December 23, 1980 to Papahadjopoulos et al.

II. Expression of H pylori Nucleic Acids Nucleic acid isolated or synthesized in accordance with the sequences described herein have utility to generate polypeptides. The nucleic acid of the invention exemplified in the Sequence Listing or fragments of said nucleic acid encoding active portions of H pylori polypeptides can be cloned into suitable vectors or used to isolate nucleic acid. The isolated nucleic acid is combined with suitable DNA linkers and cloned into a suitable vector.

The function of a specific gene or operon can be ascertained by expression in a bacterial strain under conditions where the activity of the gene product(s) specified by the gene or operon in question can be specifically measured. Alternatively, a gene product may be produced in large quantities in an expressing strain for use as an antigen, an industrial reagent, for structural studies, etc. This expression can be accomplished in a mutant strain which lacks the activity of the gene to be tested, or in a strain that does not produce the same gene product(s). This includes, but is not limited to other Helicobacter strains, or other bacterial strains such as E. coli, Norcardia, Corynebacterium, Campylobacter, and Streptomyces species. In some cases the expression host will utilize the natural Helicobacter promoter whereas in others, it will be necessary to drive the gene with a promoter sequence derived from the expressing organism (e.g., an E. coli beta-galactosidase promoter for expression in E. coli).

To express a gene product using the natural H. pylori promoter, a procedure such as the following can be used. A restriction fragment containing the gene of interest, together with its associated natural promoter element and regulatory sequences (identified using the DNA sequence data) is cloned into an appropriate recombinant plasmid containing an origin of replication that functions in the host organism and an appropriate selectable marker. This can be accomplished by a number of procedures known to those skilled in the art. It is most preferably done by cutting the plasmid and the fragment to be cloned with the same restriction enzyme to produce compatible ends that can be ligated to join the two pieces together. The recombinant plasmid is introduced into the host organism by, for example, electroporation and cells containing the recombinant plasmid are identified by selection for the marker on the plasmid.

Expression of the desired gene product is detected using an assay specific for that gene product.

In the case of a gene that requires a different promoter, the body of the gene (coding sequence) is specifically excised and cloned into an appropriate expression

plasmid. This subcloning can be done by several methods, but is most easily accomplished by PCR amplification of a specific fragment and ligation into an expression plasmid after treating the PCR product with a restriction enzyme or exonuclease to create suitable ends for cloning.

A suitable host cell for expression of a gene can be any procaryotic or eucaryotic cell. For example, an H. pylori polypeptide can be expressed in bacterial cells such as E.

coli, insect cells (baculovirus), yeast, or mammalian cells such as Chinese hamster ovary cell (CHO). Other suitable host cells are known to those skilled in the art.

Expression in eucaryotic cells such as mammalian, yeast, or insect cells can lead to partial or complete glycosylation and/or formation of relevant inter- or intra-chain disulfide bonds of a recombinant peptide product. Examples of vectors for expression in yeast S. cerivisae include pYepSecl (Baldari. et al., (1987) Embo J. 6:229-234), pMFa (Kurjan and Herskowitz, (1982) Cell 30:933-943), pJRY88 (Schultz et al., (1987) Gene 54:113-123), and pYES2 (Invitrogen Corporation, San Diego, CA). Baculovirus vectors available for expression of proteins in cultured insect cells (SF 9 cells) include the pAc series (Smith et al., (1983) Mol. Cell Biol. 3:2156-2165) and the pVL series (Lucklow, V.A., and Summers, M.D., (1989) Virology 170:31-39). Generally, COS cells (Gluzman, Y., (1981) Cell 23:175-182) are used in conjunction with such vectors as pCDM 8 (Aruffo, A. and Seed, B., (1987) Proc. Natl. Acad. Sci. USA 84:8573-8577) for transient amplification/expression in mammalian cells, while CHO (dhfr- Chinese Hamster Ovary) cells are used with vectors such as pMT2PC (Kaufman et al. (1987), EMBO J. 6:187-195) for stable amplification/expression in mammalian cells. Vector DNA can be introduced into mammalian cells via conventional techniques such as calcium phosphate or calcium chloride co-precipitation. DEAE-dextran-mediated transfection, or electroporation. Suitable methods for transforming host cells can be found in Sambrook et al. (Molecular Cloning: A Laboratorv Manual, 2nd Edition, Cold Spring Harbor Laboratory press (1989)), and other laboratory textbooks.

Expression in procaryotes is most often carried out in E. coli with either fusion or non-fusion inducible expression vectors. Fusion vectors usually add a number of NH2 terminal amino acids to the expressed target gene. These NH2 terminal amino acids often are referred to as a reporter group. Such reporter groups usually serve two purposes: 1) to increase the solubility of the target recombinant protein; and 2) to aid in the purification of the target recombinant protein by acting as a ligand in affinity purification. Often, in fusion expression vectors, a proteolytic cleavage site is introduced at the junction of the reporter group and the target recombinant protein to enable separation of the target recombinant protein from the reporter group subsequent to purification of the fusion protein. Such enzymes, and their cognate recognition

sequences, include Factor Xa, thrombin and enterokinase. Typical fusion expression vectors include pGEX (Amrad Corp., Melbourne, Australia), pMAL (New England Biolabs, Beverly, MA) and pKIT5 (Pharmacia, Piscataway, NJ) which fuse glutathione S-transferase, maltose E binding protein, or protein A, respectively, to the target recombinant protein. A preferred reporter group is poly(His), which may be fused to the amino or carboxy terminus of the protein and which renders the recombinant fusion protein easily purifiable by metal chelate chromatography.

Inducible non-fusion expression vectors include pTrc (Amann et al., (1988) Gene 69:301-315) and pETl ld (Studier et al., Gene Expression Technology: Methods in Enzvmology 185, Academic Press, San Diego, California (1990) 60-89). While target gene expression relies on host RNA polymerase transcription from the hybrid trp-lac fusion promoter in pTrc, expression of target genes inserted into pET1 1d relies on transcription from the T7 gnlO-lac 0 fusion promoter mediated by coexpressed viral RNA polymerase (T7 gnl). This viral polymerase is supplied by host strains BL21(DE3) or HMS174(DE3) from a resident X prophage harboring a T7 gnl under the transcriptional control of the lacUV 5 promoter.

For example, a host cell transfected with a nucleic acid vector directing expression of a nucleotide sequence encoding an H. pylori polypeptide can be cultured under appropriate conditions to allow expression of the polypeptide to occur. The polypeptide may be secreted and isolated from a mixture of cells and medium containing the peptide. Alternatively, the polypeptide may be retained cytoplasmically and the cells harvested, lysed and the protein isolated. A cell culture includes host cells, media and other byproducts. Suitable media for cell culture are well known in the art.

Polypeptides of the invention can be isolated from cell culture medium, host cells, or both using techniques known in the art for purifying proteins including ion-exchange chromatography, gel filtration chromatography, ultrafiltration, electrophoresis, and immunoaffinity purification with antibodies specific for such polypeptides.

Additionally, in many situations, polypeptides can be produced by chemical cleavage of a native protein (e.g., tryptic digestion) and the cleavage products can then be purified by standard techniques.

In the case of membrane bound proteins, these can be isolated from a host cell by contacting a membrane-associated protein fraction with a detergent forming a solubilized complex, where the membrane-associated protein is no longer entirely embedded in the membrane fraction and is solubilized at least to an extent which allows it to be chromatographically isolated from the membrane fraction. Several different criteria are used for choosing a detergent suitable for solubilizing these complexes. For example, one property considered is the ability of the detergent to solubilize the H.

pylori protein within the membrane fraction at minimal denaturation of the membrane- associated protein allowing for the activity or functionality of the membrane-associated protein to return upon reconstitution of the protein. Another property considered when selecting the detergent is the critical micelle concentration (CMC) of the detergent in that the detergent of choice preferably has a high CMC value allowing for ease of removal after reconstitution. A third property considered when selecting a detergent is the hydrophobicity of the detergent. Typically, membrane-associated proteins are very hydrophobic and therefore detergents which are also hydrophobic, e.g., the triton series, would be useful for solubilizing the hydrophobic proteins. Another property important to a detergent can be the capability of the detergent to remove the H. pylori protein with minimal protein-protein interaction facilitating further purification. A fifth property of the detergent which should be considered is the charge of the detergent. For example, if it is desired to use ion exchange resins in the purification process then preferably detergent should be an uncharged detergent. Chromatographic techniques which can be used in the final purification step are known in the art and include hydrophobic interaction, lectin affinity, ion exchange, dye affinity and immunoaffinity.

One strategy to maximize recombinant H. pylori peptide expression in E. coli is to express the protein in a host bacteria with an impaired capacity to proteolytically cleave the recombinant protein (Gottesman, S., Gene Expression Technolov: Methods in Enzvmologv 185, Academic Press, San Diego, California (1990)119-128). Another strategy would be to alter the nucleic acid encoding an H. pylori peptide to be inserted into an expression vector so that the individual codons for each amino acid would be those preferentially utilized in highly expressed E. coli proteins (Wada et al., (1992) Nuc. Acids Res. 20:2111-2118). Such alteration of nucleic acids of the invention can be carried out by standard DNA synthesis techniques.

The nucleic acids of the invention can also be chemically synthesized using standard techniques. Various methods of chemically synthesizing polydeoxynucleotides are known, including solid-phase synthesis which, like peptide synthesis, has been fully automated in commercially available DNA synthesizers (See, e.g., Itakura et al. U.S.

Patent No. 4,598,049; Caruthers et al. U.S. Patent No. 4,458,066; and Itakura U.S.

Patent Nos. 4,401,796 and 4,373,071, incorporated by reference herein).

III. H. pylori Polypeptides This invention encompasses isolated H. pylori polypeptides encoded by the disclosed H. pylori genomic sequences, including the polypeptides of the invention contained in the Sequence Listing. Polypeptides of the invention are preferably at least 5 amino acid residues in length. Using the DNA sequence information provided herein,

the amino acid sequences of the polypeptides encompassed by the invention can be deduced using methods well-known in the art. It will be understood that the sequence of an entire nucleic acid encoding an H. pylori polypeptide can be isolated and identified based on an ORF that encodes only a fragment of the cognate protein-coding region.

This can be acheived, for example, by using the isolated nucleic acid encoding the ORF, or fragments thereof, to prime a polymerase chain reaction with genomic H. pylori DNA as template; this is followed by sequencing the amplified product.

The polypeptides of the invention can be isolated from wild-type or mutant H.

pylori cells or from heterologous organisms or cells (including, but not limited to, bacteria, yeast, insect, plant and mammalian cells) into which an H. pylori nucleic acid has been introduced and expressed. In addition, the polypeptides can be part of recombinant fusion proteins.

H. pylori polypeptides of the invention can be chemically synthesized using commercially automated procedures such as those referenced herein.

IV. Identification of Nucleic Acids Encoding Vaccine Components and Targets for Agents Effective Against H. pvlori The disclosed H. pylori genome sequence includes segments that direct the synthesis of ribonucleic acids and polypeptides, as well as origins of replication, promoters, other types of regulatory sequences, and intergenic nucleic acids. The invention encompasses nucleic acids encoding immunogenic components of vaccines and targets for agents effective against H. pylori. Identification of said immunogenic components involved in the determination of the function of the disclosed sequences can be achieved using a variety of approaches. Non-limiting examples of these approaches are described briefly below.

Homologv to known sequences: Computer-assisted comparison of the disclosed H. pylori sequences with previously reported sequences present in publicly available databases is useful for identifying functional H. pylori nucleic acid and polypeptide sequences. It will be understood that protein-coding sequences, for example, may be compared as a whole, and that a high degree of sequence homology between two proteins (such as, for example, >80-90%) at the amino acid level indicates that the two proteins also possess some degree of functional homology, such as, for example, among enzymes involved in metabolism, DNA synthesis, or cell wall synthesis, and proteins involved in transport, cell division, etc. In addition, many structural features of particular protein classes have been identified and correlate with specific consensus sequences, such as, for example, binding domains for nucleotides, DNA, metal ions, and other small molecules; sites for covalent modifications such as phosphorylation,

acylation, and the like; sites of protein:protein interactions, etc. These consensus sequences may be quite short and thus may represent only a fraction of the entire protein-coding sequence. Identification of such a feature in an H. pylori sequence is therefore useful in determining the function of the encoded protein and identifying useful targets of antibacterial drugs.

Of particular relevance to the present invention are structural features that are common to secretory, transmembrane, and surface proteins, including secretion signal peptides and hydrophobic transmembrane domains. H. pylori proteins identified as containing putative signal sequences and/or transmembrane domains are useful as immunogenic components of vaccines.

Identification of essential genes: Nucleic acids that encode proteins essential for growth or viability of H pylori are preferred drug targets. H. pylori genes can be tested for their biological relevance to the organism by examining the effect of deleting and/or disrupting the genes, i.e., by so-called gene "knockout", using techniques known to those skilled in the relevant art. In this manner essential genes may be identified.

Strain-syecific sequences: Because of the evolutionary relationship between different H. pylori strains it is believed that the presently disclosed H. pylori sequences are useful for identifying, and/or discriminating between, previously known and new H.

pylori strains. It is believed that other H. pylori strains will exhibit at least 70% sequence homology with the presently disclosed sequence. Systematic and routine analyses of DNA sequences derived from samples containing H. pylori strains, and comparison with the present sequence allows for the identification of sequences that can be used to discriminate between strains as well as those that are common to all H. pylori strains. In one embodiment, the invention provides nucleic acids, including probes, and peptide and polypeptide sequences that discriminate between different strains of H pylori. Strain-specific components can also be identified functionally by their ability to elicit or react with antibodies that selectively recognize one or more H. pylori strains.

In another embodiment the invention provides nucleic acids, including probes, and peptide and polypeptide sequences that are common to all H. pylori strains but are not found in other bacterial species.

Specific Example: Determination Of Candidate Protein Antigens For Antibodv And Vaccine Development The selection of candidate protein antigens for vaccine development can be derived from the nucleic acids encoding H. pylori polypeptides. First, the ORF's can be analyzed for homology to other known exported or membrane proteins and analyzed using the discriminant analysis described by Klein, et al. (Klein, P., Kanehsia, M., and

DeLisi, C. (1985) Biochimica et Biophysica Acta 815, 468-476) for predicting exported and membrane proteins.

Homology searches can be performed using the BLAST algorithm contained in the Wisconsin Sequence Analysis Package (Genetics Computer Group, University Research Park, 575 Science Drive, Madison, WI 53711) to compare each predicted ORF amino acid sequence with all sequences found in the current GenBank, SWISS-PROT and PIR databases. BLAST searches for local alignments between the ORF and the databank sequences and reports a probability score which indicates the probability of finding this sequence by chance in the database. ORF's with significant homology (e.g.

probabilities lower than lx 10-6 that the homology is only due to random chance) to membrane or exported proteins represent protein antigens for vaccine development.

Possible functions can be provided to H. pylori genes based on sequence homology to genes cloned in other organisms.

Discriminant analysis (Klein, et al. supra) can be used to examine the ORF amino acid sequences. This algorithm uses the intrinsic information contained in the ORF amino acid sequence and compares it to information derived from the properties of known membrane and exported proteins. This comparison predicts which proteins will be exported, membrane associated or cytoplasmic. ORF amino acid sequences identified as exported or membrane associated by this algorithm are likely protein antigens for vaccine development.

Surface exposed outer membrane proteins are likely to represent the best antigens to provide a protective immune response against H. pylori. Among the algorithms that can be used to aid in prediction of these outer membrane proteins include the presence of an amphipathic beta-sheet region at their C-terminus. This region which has been detected in a large number of outer membrane proteins in Gram negative bacteria is often characterized by hydrophobic residues (Phe or Tyr) clustered at alternating positions from the C-terminus (e.g., see Figure 5, block F; Figure 7, block E).

Importantly, these sequences have not been detected at the C-termini of periplasmic proteins, thus allowing preliminary distinction between these classes of proteins based on primary sequence data. This phenomenon has been reported previously by Struyve et al. (J. Mol. Biol. 218:141-148, 1991).

Also illustrated in Figure 5 are additional amino acid sequence motifs found in many outer membrane proteins of H pylori. The amino acid sequence alignment in Figure 5 depicts portions of the sequence of five H. pylori proteins (depicted in the single letter amino acid code) labeled with their amino acid Sequence ID Numbers and shown N-terminal to C-terminal, left to right. Five or six distinct blocks (labeled A through E or F) of similar amino acid residues are found including the distinctive

hydrophobic residues (Phe or Tyr; F or Y according to the single letter code for amino acid residues) frequently found at positions near the C-terminus of outer membrane proteins. The presence of several shared motifs clearly establishes the similarity between members of this group of proteins.

Additional amino acid alignments for four outer membrane proteins isolated from H. pylori are depicted in Figure 6.

Outer membrane proteins isolated from H. pylori frequently share additional motifs as depicted for two proteins in Figure 7 which also share the C-terminal hydrophobic residues, and as depicted for two proteins in Figure 8 which do not share the C-terminal hydrophobic residue motif but share a different C-terminal motif.

One skilled in the art would know that these shared sequence motifs are highly significant and establish a similarity among this group of proteins.

Infrequently it is not possible to distinguish between multiple possible nucleotides at a given position in the nucleic acid sequence. In those cases the ambiguities are denoted by an extended alphabet as follows: These are the official IUPAC-IUB single-letter base codes Code Base Description G Guanine A Adenine T Thymine C Cytosine R Purine (A or G) Y Pyrimidine (C or T or U) M Amino (A or C) K Ketone (G or T) S Strong interaction (C or G) W Weak interaction (A or T) H Not-G (A or C or T) B Not-A (C or G or T) V Not-T (not-U) (A or C or G) D Not-C (A or G or T) N Any (A or C or G or T) The amino acid translations of this invention account for the ambiguity in the nucleic acid sequence by translating the ambiguous codon as the letter "X". In all cases,

the permissible amino acid residues at a position are clear from an examination of the nucleic acid sequence based on the standard genetic code.

V. Production of Fragments and Analogs of H. pylori Nucleic Acids and Polypeptides Based on the discovery of the H. pylori gene products of the invention provided in the Sequence Lsiting, one skilled in the art can alter the disclosed structure (of H.

pylori genes), e.g., by producing fragments or analogs, and test the newly produced structures for activity. Examples of techniques known to those skilled in the relevant art which allow the production and testing of fragments and analogs are discussed below.

These, or analogous methods can be used to make and screen libraries of polypeptides, e.g., libraries of random peptides or libraries of fragments or analogs of cellular proteins for the ability to bind H. pylori polypeptides. Such screens are useful for the identification of inhibitors of H. pylori.

Generation of Fragments Fragments of a protein can be produced in several ways, e.g., recombinantly, by proteolytic digestion, or by chemical synthesis. Internal or terminal fragments of a polypeptide can be generated by removing one or more nucleotides from one end (for a terminal fragment) or both ends (for an internal fragment) of a nucleic acid which encodes the polypeptide. Expression of the mutagenized DNA produces polypeptide fragments. Digestion with "end-nibbling" endonucleases can thus generate DNA's which encode an array of fragments. DNA's which encode fragments of a protein can also be generated by random shearing, restriction digestion or a combination of the above-discussed methods.

Fragments can also be chemically synthesized using techniques known in the art such as conventional Merrifield solid phase f-Moc or t-Boc chemistry. For example, peptides of the present invention may be arbitrarily divided into fragments of desired length with no overlap of the fragments, or divided into overlapping fragments of a desired length.

Alteration of Nucleic Acids and Polypeptides: Random Methods Amino acid sequence variants of a protein can be prepared by random mutagenesis of DNA which encodes a protein or a particular domain or region of a protein. Useful methods include PCR mutagenesis and saturation mutagenesis. A library of random amino acid sequence variants can also be generated by the synthesis of a set of degenerate oligonucleotide sequences. (Methods for screening proteins in a library of variants are elsewhere herein).

(A) PCR Mutagenesis In PCR mutagenesis. reduced Taq polymerase fidelity is used to introduce random mutations into a cloned fragment of DNA (Leung et al., 1989. Technique 1:11 - 15). The DNA region to be mutagenized is amplified using the polymerase chain reaction (PCR) under conditions that reduce the fidelity of DNA synthesis by Taq DNA polymerase, e.g., by using a dGTP/dATP ratio of five and adding Mn2+ to the PCR reaction. The pool of amplified DNA fragments are inserted into appropriate cloning vectors to provide random mutant libraries.

(B) Saturation Mutagenesis Saturation mutagenesis allows for the rapid introduction of a large number of single base substitutions into cloned DNA fragments (Mayers et al., 1985, Science 229:242). This technique includes generation of mutations, e.g., by chemical treatment or irradiation of single-stranded DNA in vitro, and synthesis of a complimentary DNA strand. The mutation frequency can be modulated by modulating the severity of the treatment, and essentially all possible base substitutions can be obtained. Because this procedure does not involve a genetic selection for mutant fragments both neutral substitutions, as well as those that alter function, are obtained. The distribution of point mutations is not biased toward conserved sequence elements.

(C) Degenerate Oligonucleotides A library of homologs can also be generated from a set of degenerate oligonucleotide sequences. Chemical synthesis of a degenerate sequences can be carried out in an automatic DNA synthesizer, and the synthetic genes then ligated into an appropriate expression vector. The synthesis of degenerate oligonucleotides is known in the art (see for example, Narang, SA (1983) Tetrahedron 39:3; Itakura et al. (1981) Recombinant DNA, Proc 3rd Cleveland Sympos. Macromolecules, ed. AG Walton, Amsterdam: Elsevier pp273-289; Itakura et al. (1984) Annu. Rev. Biochem. 53:323; Itakura et al. (1984) Science 198:1056; Ike et al. (1983) Nucleic Acid Res. 11:477. Such techniques have been employed in the directed evolution of other proteins (see, for example, Scott et al. (1990) Science 249:386-390; Roberts et al. (1992) PNAS 89:2429- 2433; Devlin et al. (1990) Science 249: 404-406; Cwirla et al. (1990) PNAS 87: 6378- 6382; as well as U.S. Patents Nos. 5,223,409, 5,198,346, and 5,096,815).

Alteration of Nucleic Acids and Polypeptides: Methods for Directed Mutagenesis Non-random or directed, mutagenesis techniques can be used to provide specific sequences or mutations in specific regions. These techniques can be used to create variants which include, e.g., deletions, insertions, or substitutions, of residues of the known amino acid sequence of a protein. The sites for mutation can be modified individually or in series, e.g., by (1) substituting first with conserved amino acids and then with more radical choices depending upon results achieved, (2) deleting the target residue, or (3) inserting residues of the same or a different class adjacent to the located site, or combinations of options 1-3.

(A) Alanine Scanning Mutagenesis Alanine scanning mutagenesis is a useful method for identification of certain residues or regions of the desired protein that are preferred locations or domains for mutagenesis, Cunningham and Wells (Science 244:1081-1085, 1989). In alanine scanning, a residue or group of target residues are identified (e.g., charged residues such as Arg, Asp, His, Lys, and Glu) and replaced by a neutral or negatively charged amino acid (most preferably alanine or polyalanine). Replacement of an amino acid can affect the interaction of the amino acids with the surrounding aqueous environment in or outside the cell. Those domains demonstrating functional sensitivity to the substitutions are then refined by introducing further or other variants at or for the sites of substitution.

Thus, while the site for introducing an amino acid sequence variation is predetermined, the nature of the mutation per se need not be predetermined. For example, to optimize the performance of a mutation at a given site, alanine scanning or random mutagenesis may be conducted at the target codon or region and the expressed desired protein subunit variants are screened for the optimal combination of desired activity.

(B) Olinonucleotide-Mediated Mutagenesis Oligonucleotide-mediated mutagenesis is a useful method for preparing substitution, deletion, and insertion variants of DNA, see, e.g., Adelman et al., (DNA 2:183, 1983). Briefly, the desired DNA is altered by hybridizing an oligonucleotide encoding a mutation to a DNA template, where the template is the single-stranded form of a plasmid or bacteriophage containing the unaltered or native DNA sequence of the desired protein. After hybridization, a DNA polymerase is used to synthesize an entire second complementary strand of the template that will thus incorporate the oligonucleotide primer, and will code for the selected alteration in the desired protein DNA. Generally, oligonucleotides of at least 25 nucleotides in length are used. An optimal oligonucleotide will have 12 to 15 nucleotides that are completely

complementary to the template on either side of the nucleotide(s) coding for the mutation. This ensures that the oligonucleotide will hybridize properly to the single- stranded DNA template molecule. The oligonucleotides are readily synthesized using techniques known in the art such as that described by Crea et al. (Proc. Natl. Acad. Sci.

USA, 75: 5765[1978]).

(C) Cassette Mutagenesis Another method for preparing variants, cassette mutagenesis, is based on the technique described by Wells et al. (Gene, 34:315[1985]). The starting material is a plasmid (or other vector) which includes the protein subunit DNA to be mutated. The codon(s) in the protein subunit DNA to be mutated are identified. There must be a unique restriction endonuclease site on each side of the identified mutation site(s). If no such restriction sites exist, they may be generated using the above-described oligonucleotide-mediated mutagenesis method to introduce them at appropriate locations in the desired protein subunit DNA. After the restriction sites have been introduced into the plasmid, the plasmid is cut at these sites to linearize it. A double-stranded oligonucleotide encoding the sequence of the DNA between the restriction sites but containing the desired mutation(s) is synthesized using standard procedures. The two strands are synthesized separately and then hybridized together using standard techniques. This double-stranded oligonucleotide is referred to as the cassette. This cassette is designed to have 3' and 5' ends that are comparable with the ends of the linearized plasmid, such that it can be directly ligated to the plasmid. This plasmid now contains the mutated desired protein subunit DNA sequence.

(D) Combinatorial Mutagenesis Combinatorial mutagenesis can also be used to generate mutants (Ladner et al., WO 88/06630). In this method, the amino acid sequences for a group of homologs or other related proteins are aligned, preferably to promote the highest homology possible.

All of the amino acids which appear at a given position of the aligned sequences can be selected to create a degenerate set of combinatorial sequences. The variegated library of variants is generated by combinatorial mutagenesis at the nucleic acid level, and is encoded by a variegated gene library. For example, a mixture of synthetic oligonucleotides can be enzymatically ligated into gene sequences such that the degenerate set of potential sequences are expressible as individual peptides, or alternatively, as a set of larger fusion proteins containing the set of degenerate sequences.

Other Modifications of H. pylori Nucleic Acids and Polvpeptides It is possible to modify the structure of an H. pylori polypeptide for such purposes as increasing solubility, enhancing stability (e.g., shelf life ex vivo and resistance to proteolytic degradation in vivo). A modified H. pylori protein or peptide can be produced in which the amino acid sequence has been altered, such as by amino acid substitution, deletion, or addition as described herein.

An H. pylori peptide can also be modified by substitution of cysteine residues preferably with alanine, serine, threonine, leucine or glutamic acid residues to minimize dimerization via disulfide linkages. In addition, amino acid side chains of fragments of the protein of the invention can be chemically modified. Another modification is cyclization of the peptide.

In order to enhance stability and/or activity an H. pylori polypeptide can be modified to incorporate one or more polymorphisms in the amino acid sequence of the protein resulting from any natural allelic variation. Additionally, D-amino acids, non- natural amino acids, or non-amino acid analogs can be substituted or added to produce a modified protein within the scope of this invention. Furthermore, an H. pylori polypeptide can be modified using polyethylene glycol (PEG) according to the method of A. Sehon and co-workers (Wie et al., supra) to produce a protein conjugated with PEG. In addition, PEG can be added during chemical synthesis of the protein. Other modifications of H pylori proteins include reduction/alkylation (Tarr, Methods of Protein Microcharacterization, J. E. Silver ed., Humana Press, Clifton NJ 155-194 (1986)); acylation (Tarr, supra); chemical coupling to an appropriate carrier (Mishell and Shiigi, eds, Selected Methods in Cellular Immunology, WH Freeman, San Francisco, CA (1980), U.S. Patent 4,939,239; or mild formalin treatment (Marsh, (1971) Lint. Arch. of AllergyandAppl. Immunol., 41: 199 - 215).

To facilitate purification and potentially increase solubility of an H. pylori protein or peptide, it is possible to add an amino acid fusion moiety to the peptide backbone. For example, hexa-histidine can be added to the protein for purification by immobilized metal ion affinity chromatography (Hochuli, E. et al., (1988) Bio/Technology, 6: 1321 - 1325). In addition, to facilitate isolation of peptides free of irrelevant sequences, specific endoprotease cleavage sites can be introduced between the sequences of the fusion moiety and the peptide.

To potentially aid proper antigen processing of epitopes within an H. pylori polypeptide, canonical protease sensitive sites can be engineered between regions, each comprising at least one epitope via recombinant or synthetic methods. For example, charged amino acid pairs, such as KK or RR, can be introduced between regions within a protein or fragment during recombinant construction thereof. The resulting peptide

can be rendered sensitive to cleavage by cathepsin and/or other trypsin-like enzymes which would generate portions of the protein containing one or more epitopes. In addition, such charged amino acid residues can result in an increase in the solubility of the peptide.

Primarv Methods for Screening Polypeptides and Analogs Various techniques are known in the art for screening generated mutant gene products. Techniques for screening large gene libraries often include cloning the gene library into replicable expression vectors transforming appropriate cells with the resulting library of vectors, and expressing the genes under conditions in which detection of a desired activity, e.g., in this case, binding to H. pylori polypeptide or an interacting protein. facilitates relatively easy isolation of the vector encoding the gene whose product was detected. Each of the techniques described below is amenable to high through-put analysis for screening large numbers of sequences created, e.g., by random mutagenesis techniques.

(A) Two Hvbrid Systems Two hybrid assays such as the system described above (as with the other screening methods described herein), can be used to identify polypeptides, e.g., fragments or analogs of a naturally-occurring H. pylori polypeptide, e.g., of cellular proteins, or of randomly generated polypeptides which bind to an H. pylori protein.

(The H. pylori domain is used as the bait protein and the library of variants are expressed as fish fusion proteins.) In an analogous fashion, a two hybrid assay (as with the other screening methods described herein), can be used to find polypeptides which bind a H.

pylori polypeptide.

(B) Display Libraries In one approach to screening assays, the candidate peptides are displayed on the surface of a cell or viral particle, and the ability of particular cells or viral particles to bind an appropriate receptor protein via the displayed product is detected in a "panning assay". For example, the gene library can be cloned into the gene for a surface membrane protein of a bacterial cell, and the resulting fusion protein detected by panning (Ladner et al., WO 88/06630; Fuchs et al. (1991) Bio/Technology 9:1370-1371; and Goward et al. (1992) TIBS 18:136-140). In a similar fashion, a detectably labeled ligand can be used to score for potentially functional peptide homologs. Fluorescently labeled ligands, e.g., receptors. can be used to detect homologs which retain ligand- binding activity. The use of fluorescently labeled ligands, allows cells to be visually

inspected and separated under a fluorescence microscope, or, where the morphology of the cell permits, to be separated by a fluorescence-activated cell sorter.

A gene library can be expressed as a fusion protein on the surface of a viral particle. For instance, in the filamentous phage system, foreign peptide sequences can be expressed on the surface of infectious phage, thereby conferring two significant benefits. First, since these phage can be applied to affinity matrices at concentrations well over 1013 phage per milliliter, a large number of phage can be screened at one time.

Second, since each infectious phage displays a gene product on its surface, if a particular phage is recovered from an affinity matrix in low yield, the phage can be amplified by another round of infection. The group of almost identical E. coli filamentous phages M13, fd., and fl are most often used in phage display libraries. Either of the phage gIII or gVIII coat proteins can be used to generate fusion proteins without disrupting the ultimate packaging of the viral particle. Foreign epitopes can be expressed at the NH2- terminal end of pIll and phage bearing such epitopes recovered from a large excess of phage lacking this epitope (Ladner et al. PCT publication WO 90/02909; Garrard et al., PCT publication WO 92/09690; Marks et al. (1992) J. Biol. Chem. 267:16007-16010; Griffiths et al. (1993) EMBOJ 12:725-734; Clackson et al. (1991) Nature 352:624-628; and Barbas et al. (1992) PNAS 89:4457-4461).

A common approach uses the maltose receptor of E. coli (the outer membrane protein, LamB) as a peptide fusion partner (Charbit et al. (1986) EMBO 5, 3029-3037).

Oligonucleotides have been inserted into plasmids encoding the LamB gene to produce peptides fused into one of the extracellular loops of the protein. These peptides are available for binding to ligands e.g., to antibodies, and can elicit an immune response when the cells are administered to animals. Other cell surface proteins, e.g., OmpA (Schorr et al. (1991) Vaccines 91, pp. 387-392), PhoE (Agterberg, et al. (1990) Gene 88, 37-45), and PAL (Fuchs et al. (1991) Bio/Tech 9, 1369-1372), as well as large bacterial surface structures have served as vehicles for peptide display. Peptides can be fused to pilin, a protein which polymerizes to form the pilus-a conduit for interbacterial exchange of genetic information (Thiry et al. (1989) Appl. Environ. Microbiol. 55, 984-993).

Because of its role in interacting with other cells, the pilus provides a useful support for the presentation of peptides to the extracellular environment. Another large surface structure used for peptide display is the bacterial motive organ, the flagellum. Fusion of peptides to the subunit protein flagellin offers a dense array of many peptide copies on the host cells (Kuwajima et al. (1988) Bio/Tech. 6, 1080-1083). Surface proteins of other bacterial species have also served as peptide fusion partners. Examples include the Staphylococcus protein A and the outer membrane IgA protease of Neisseria (Hansson

et al. (1992) J. Bacteriol. 174, 4239-4245 and Klauser et al. (1990) EMBO J. 9, 1991- 1999).

In the filamentous phage systems and the LamB system described above, the physical link between the peptide and its encoding DNA occurs by the containment of the DNA within a particle (cell or phage) that carries the peptide on its surface.

Capturing the peptide captures the particle and the DNA within. An alternative scheme uses the DNA-binding protein LacI to form a link between peptide and DNA (Cull et al.

(1992) PNAS USA 89:1865-1869). This system uses a plasmid containing the LacI gene with an oligonucleotide cloning site at its 3'-end. Under the controlled induction by arabinose, a LacI-peptide fusion protein is produced. This fusion retains the natural ability of LacI to bind to a short DNA sequence known as LacO operator (LacO). By installing two copies of LacO on the expression plasmid, the LacI-peptide fusion binds tightly to the plasmid that encoded it. Because the plasmids in each cell contain only a single oligonucleotide sequence and each cell expresses only a single peptide sequence, the peptides become specifically and stably associated with the DNA sequence that directed its synthesis. The cells of the library are gently lysed and the peptide-DNA complexes are exposed to a matrix of immobilized receptor to recover the complexes containing active peptides. The associated plasmid DNA is then reintroduced into cells for amplification and DNA sequencing to determine the identity of the peptide ligands.

As a demonstration of the practical utility of the method, a large random library of dodecapeptides was made and selected on a monoclonal antibody raised against the opioid peptide dynorphin B. A cohort of peptides was recovered, all related by a consensus sequence corresponding to a six-residue portion of dynorphin B. (Cull et al.

(1992) Proc. Natl. Acad Sci. USA. 89-1869) This scheme, sometimes referred to as peptides-on-plasmids, differs in two important ways from the phage display methods. First, the peptides are attached to the C-terminus of the fusion protein, resulting in the display of the library members as peptides having free carboxy termini. Both of the filamentous phage coat proteins, pIII and pVIII, are anchored to the phage through their C-termini, and the guest peptides are placed into the outward-extending N-terminal domains. In some designs, the phage- displayed peptides are presented right at the amino terminus of the fusion protein.

(Cwirla, et al. (1990) Proc. Natl. Acad Sci. U.S.A. 87, 6378-6382) A second difference is the set of biological biases affecting the population of peptides actually present in the libraries. The LacI fusion molecules are confined to the cytoplasm of the host cells.

The phage coat fusions are exposed briefly to the cytoplasm during translation but are rapidly secreted through the inner membrane into the periplasmic compartment, remaining anchored in the membrane by their C-terminal hydrophobic domains, with the

N-termini, containing the peptides, protruding into the periplasm while awaiting assembly into phage particles. The peptides in the LacI and phage libraries may differ significantly as a result of their exposure to different proteolytic activities. The phage coat proteins require transport across the inner membrane and signal peptidase processing as a prelude to incorporation into phage. Certain peptides exert a deleterious effect on these processes and are underrepresented in the libraries (Gallop et al. (1994) J.

Med. Chem. 37(9):1233-1251). These particular biases are not a factor in the LacI display system.

The number of small peptides available in recombinant random libraries is enormous. Libraries of 107-109 independent clones are routinely prepared. Libraries as large as 1011 recombinants have been created, but this size approaches the practical limit for clone libraries. This limitation in library size occurs at the step of transforming the DNA containing randomized segments into the host bacterial cells. To circumvent this limitation, an in vitro system based on the display of nascent peptides in polysome complexes has recently been developed. This display library method has the potential of producing libraries 3-6 orders of magnitude larger than the currently available phage/phagemid or plasmid libraries. Furthermore, the construction of the libraries, expression of the peptides, and screening, is done in an entirely cell-free format.

In one application of this method (Gallop et al. (1994) J. Med. Chem.

37(9):1233-1251), a molecular DNA library encoding 1012 decapeptides was constructed and the library expressed in an E. coli S30 in vitro coupled transcription/translation system. Conditions were chosen to stall the ribosomes on the mRNA, causing the accumulation of a substantial proportion of the RNA in polysomes and yielding complexes containing nascent peptides still linked to their encoding RNA.

The polysomes are sufficiently robust to be affinity purified on immobilized receptors in much the same way as the more conventional recombinant peptide display libraries are screened. RNA from the bound complexes is recovered, converted to cDNA, and amplified by PCR to produce a template for the next round of synthesis and screening.

The polysome display method can be coupled to the phage display system. Following several rounds of screening, cDNA from the enriched pool of polysomes was cloned into a phagemid vector. This vector serves as both a peptide expression vector, displaying peptides fused to the coat proteins, and as a DNA sequencing vector for peptide identification. By expressing the polysome-derived peptides on phage, one can either continue the affinity selection procedure in this format or assay the peptides on individual clones for binding activity in a phage ELISA, or for binding specificity in a completion phage ELISA (Barret, et al. (1992) Anal. Biochem 204,357-364). To

identify the sequences of the active peptides one sequences the DNA produced by the phagemid host.

Secondarv Screening of Polypeptides and Analogs The high through-put assays described above can be followed by secondary screens in order to identify further biological activities which will, e.g., allow one skilled in the art to differentiate agonists from antagonists. The type of a secondary screen used will depend on the desired activity that needs to be tested. For example, an assay can be developed in which the ability to inhibit an interaction between a protein of interest and its respective ligand can be used to identify antagonists from a group of peptide fragments isolated though one of the primary screens described above.

Therefore, methods for generating fragments and analogs and testing them for activity are known in the art. Once the core sequence of interest is identified, it is routine for one skilled in the art to obtain analogs and fragments.

Peptide Mimetics of H pylori Polypeptides The invention also provides for reduction of the protein binding domains of the subject H. pylori polypeptides to generate mimetics, e.g. peptide or non-peptide agents.

The peptide mimetics are able to disrupt binding of a polypeptide to its counter ligand, e.g., in the case of an H. pylori polypeptide binding to a naturally occurring ligand. The critical residues of a subject H. pylori polypeptide which are involved in molecular recognition of a polypeptide can be determined and used to generate H. pylori-derived peptidomimetics which competitively or noncompetitively inhibit binding of the H.

pylori polypeptide with an interacting polypeptide (see, for example, European patent applications EP-412,762A and EP-B31,080A).

For example, scanning mutagenesis can be used to map the amino acid residues of a particular H. pylori polypeptide involved in binding an interacting polypeptide, peptidomimetic compounds (e.g. diazepine or isoquinoline derivatives) can be generated which mimic those residues in binding to an interacting polypeptide, and which therefore can inhibit binding of an H. pylori polypeptide to an interacting polypeptide and thereby interfere with the function of H pylori polypeptide. For instance, non- hydrolyzable peptide analogs of such residues can be generated using benzodiazepine (e.g., see Freidinger et al. in Peptides: Chemistry and Biology, G.R. Marshall ed., ESCOM Publisher: Leiden, Netherlands, 1988), azepine (e.g., see Huffman et al. in Peptides: Chemistry and Biology, G.R. Marshall ed., ESCOM Publisher: Leiden, Netherlands, 1988), substituted gama lactam rings (Garvey et al. in Peptides: Chemistry and Biology, G.R. Marshall ed., ESCOM Publisher: Leiden, Netherlands, 1988), keto-

methylene pseudopeptides (Ewenson et al. (1986) JMed Chem 29:295; and Ewenson et al. in Peptides.. Structure and Function (Proceedings of the 9th American Peptide Symposium) Pierce Chemical Co. Rockland, IL, 1985), p-turn dipeptide cores (Nagai et al. (1985) Tetrahedron Lett 26:647; and Sato et al. (1986) J Chem Soc Perkin Trans 1:1231), and -aminoalcohols (Gordon et al. (1985) Biochem Biophys Res Communl26:419; and Dann et al. (1986) Biochem Biophys Res Commun 134:71).

VI. Vaccine Formulations for H. pylori Nucleic Acids and Polypeptides This invention also features vaccine compositions or formulations (used interchangeably herein) for protection against infection by TI. pylori or for treatment of H. pylori infection. As used herein, the term "treatment of H pylori infection" refers to therapeutic treatment of an existing or established H. pylori infection. The terms "protection against H. pylori infection" or "prophylactic treatment" refer to the use of H pylori vaccine formulation for reducing the risk of or preventing an infection in a subject at risk for H. pylori infection. In one embodiment, the vaccine compositions contain one or more immunogenic components, such as a surface protein, from H. pylori, or portion thereof, and a pharmaceutically acceptable carrier. For example, in one embodiment, the vaccine formulations of the invention contain at least one or combination of H. pylori polypeptides or fragments thereof, from same or different H. pylori antigens. Nucleic acids and H. pylori polypeptides for use in the vaccine formulations of the invention include the nucleic acids and polypeptides set forth in the Sequence Listing, preferably those H. pylori nucleic acids that encode surface proteins and surface proteins or fragments thereof. For example a preferred nucleic acid and H. pylori polypeptide for use in a vaccine composition of the invention is selected from the group of nucleic acids which encode cell envelope proteins and H. pylori cell envelope proteins as set forth in Table 1. However, any nucleic acid encoding an immunogenic H pylori protein and H.

pylori polypetide, or portion thereof, can be used in the present invention. These vaccines have therapeutic and/or prophylactic utilities.

One aspect of the invention provides a vaccine composition for protection against infection by H. pylori which contains at least one immunogenic fragment of an H. pylori protein and a pharmaceutically acceptable carrier. Preferred fragments include peptides of at least about 10 amino acid residues in length, preferably about 10-20 amino acid residues in length, and more preferably about 12-16 amino acid residues in length.

Immunogenic components of the invention can be obtained, for example, by screening polypeptides recombinantly produced from the corresponding fragment of the nucleic acid encoding the full-length H. pylori protein. In addition, fragments can be

chemically synthesized using techniques known in the art such as conventional Merrifield solid phase f-Moc or t-Boc chemistry.

In one embodiment, immunogenic components are identified by the ability of the peptide to stimulate T cells. Peptides which stimulate T cells, as determined by, for example, T cell proliferation or cytokine secretion are defined herein as comprising at least one T cell epitope. T cell epitopes are believed to be involved in initiation and perpetuation of the immune response to the protein allergen which is responsible for the clinical symptoms of allergy. These T cell epitopes are thought to trigger early events at the level of the T helper cell by binding to an appropriate HLA molecule on the surface of an antigen presenting cell, thereby stimulating the T cell subpopulation with the relevant T cell receptor for the epitope. These events lead to T cell proliferation, lymphokine secretion, local inflammatory reactions, recruitment of additional immune cells to the site of antigen/T cell interaction, and activation of the B cell cascade, leading to the production of antibodies. A T cell epitope is the basic element, or smallest unit of recognition by a T cell receptor, where the epitope comprises amino acids essential to receptor recognition (e.g., approximately 6 or 7 amino acid residues). Amino acid sequences which mimic those of the T cell epitopes are within the scope of this invention.

In another embodiment, immunogenic components of the invention are identified through genomic vaccination. The basic protocol is based on the idea that expression libraries consisting of all or parts of a pathogen genome, e.g., an H. pylori genome, can confer protection when used to genetically immunize a host. This expression library immunization (ELI) is analogous to expression cloning and involves reducing a genomic expression library of a pathogen, e.g., H. pylori, into plasmids that can act as genetic vaccines. The plasmids can also be designed to encode geneTic adjuvants which can dramatically stimulate the humoral response. These genetic adjuvants can be introduced at remote sites and act as well extracelluraly as intracellularly.

This is a new approach to vaccine production that has many of the advantages of live/attenuated pathogens but no risk of infection. An expression library of pathogen DNA is used to immunize a host thereby producing the effects of antigen presentation of a live vaccine without the risk. For example, in the present invention, random fragments from the H. pylori genome or from cosmid or plasmid clones, as well as PCR products from genes identified by genomic sequencing, can be used to immunize a host. The feasibility of this approach has been demonstrated with Mycoplasma pulmonis (Barry et al., Nature 377:632-635, 1995), where even partial expression libraries ofMycoplasma pulmonis, a natural pathogen in rodents, provided protection against challenge from the pathogen.

ELI is a technique that allows for production of a non-infectious multipartite vaccine, even when little is known about pathogen's biology, because ELI uses the immune system to screen candidate genes. Once isolated, these genes can be used as genetic vaccines or for development of recombinant protein vaccines. Thus, ELI allows for production of vaccines in a systematic, largely mechanized fashion.

Screening immunogenic components can be accomplished using one or more of several different assays. For example, in vitro, peptide T cell stimulatory activity is assayed by contacting a peptide known or suspected of being immunogenic with an antigen presenting cell which presents appropriate MHC molecules in a T cell culture.

Presentation of an immunogenic H. pylori peptide in association with appropriate MHC molecules to T cells in conjunction with the necessary costimulation has the effect of transmitting a signal to the T cell that induces the production of increased levels of cytokines, particularly of interleukin-2 and interleukin-4. The culture supernatant can be obtained and assayed for interleukin-2 or other known cytokines. For example, any one of several conventional assays for interleukin-2 can be employed, such as the assay described in Proc. Natl. Acad. Sci USA, 86:1333 (1989) the pertinent portions of which are incorporated herein by reference. A kit for an assay for the production of interferon is also available from Genzyme Corporation (Cambridge, MA).

Alternatively, a common assay for T cell proliferation entails measuring tritiated thymidine incorporation. The proliferation of T cells can be measured in vitro by determining the amount of 3H-labeled thymidine incorporated into the replicating DNA of cultured cells. Therefore, the rate of DNA synthesis and, in turn, the rate of cell division can be quantified.

Vaccine compositions or formulations of the invention containing one or more immunogenic components (e.g., H. pylori polypeptide or fragment thereof or nucleic acid encoding an H. pylori polypeptide or fragment thereof) preferably include a pharmaceutically acceptable carrier. The term "pharmaceutically acceptable carrier" is intended to include any and all solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, and the like, compatible with pharmaceutical administration. Suitable pharmaceutically acceptable carriers include, for example, one or more of water, saline, phosphate buffered saline, dextrose, glycerol, ethanol and the like, as well as combinations thereof. Pharmaceutically acceptable carriers may further comprise minor amounts of auxiliary substances such as wetting or emulsifying agents, preservatives or buffers, which enhance the shelf life or effectiveness of the H. pylori nucleic acid or polypeptide. For vaccine formulations of the invention containing H. pylori polypeptides, the polypeptide is preferably coadministered with a suitable adjuvant and/or a delivery system described herein.

It will be apparent to those of skill in the art that the therapeutically effective amount of DNA or protein of this invention will depend, inter alia, upon the administration schedule, the unit dose of an H. pylori nucleic acid or polypeptide administered, whether the protein or nucleic acid is administered in combination with other therapeutic agents, the immune status and health of the patient, and the therapeutic activity of the particular protein or nucleic acid.

Vaccine formulations are conventionally administered parenterally, e.g., by injection, either subcutaneously or intramuscularly. Methods for intramuscular immunization are described by Wolff et al. (1990) Science 247: 1465-1468 and by Sedegah et al. (1994) Immunology 91: 9866-9870. Other modes of administration include oral and pulmonary formulations, suppositories, and transdermal applications.

Oral immunization is preferred over parenteral methods for inducing protection against infection by H. pylori. Czinn et. al. (1993) Vaccine 11: 637-642. Oral formulations include such normally employed excipients as, for example, pharmaceutical grades of mannitol, lactose, starch, magnesium stearate, sodium saccharine, cellulose, magnesium carbonate, and the like.

In one embodiment, the vaccine formulation includes, as a pharmaceutically acceptable carrier, an adjuvant. Examples of the suitable adjuvants for use in the vaccine formulations of the invention include, but are not limited, to aluminum hydroxide; N-acetyl-muramyl--L-threonyl-D-isoglutamine (thr-MDP); N-acetyl-nor- muramyl-L-alanyl-D-isoglutamine (CGP 11637, referred to as nor-MDP); N- acetylmuramyl-L-alanyl-D-isoglutaminyl-L-alanine-2-(1 '-2'-dipalmitoyl-sn-glycero-3- hydroxyphos-phoryloxy)-ethylamine (CGP 19835A, referred to a MTP-PE); RIBI, which contains three components from bacteria; monophosphoryl lipid A; trehalose dimycoloate; cell wall skeleton (MPL + TDM + CWS) in a 2% squalene/Tween 80 emulsion; and cholera toxin. Others which may be used are non-toxic derivatives of cholera toxin, including its B subunit, and/or conjugates or genetically engineered fusions of the H. pylori polypeptide with cholera toxin or its B subunit, procholeragenoid, fungal polysaccharides, including schizophyllan, muramyl dipeptide, muramyl dipeptide derivatives, phorbol esters, labile toxin of E. coli, non-H. pylori bacterial lysates, block polymers or saponins.

In another embodiment, the vaccine formulation includes, as a pharmaceutically acceptable carrier, a delivery system. Suitable delivery systems for use in the vaccine formulations of the invention include biodegradable microcapsules or immuno- stimulating complexes (ISCOMs), cochleates, or liposomes, genetically engineered attenuated live vectors such as viruses or bacteria, and recombinant (chimeric) virus-like

particles, e.g., bluetongue. In another embodiment of the invention, the vaccine formulation includes both a delivery system and an adjuvant.

Delivery systems in humans may include enteric release capsules protecting the antigen from the acidic environment of the stomach, and including H. pylori polypeptide in an insoluble form as fusion proteins. Suitable carriers for the vaccines of the invention are enteric coated capsules and polylactide-glycolide microspheres. Suitable diluents are 0.2 N NaHCO3 and/or saline.

Vaccines of the invention can be administered as a primary prophylactic agent in adults or in children, as a secondary prevention, after successful eradication of H. pylori in an infected host, or as a therapeutic agent in the aim to induce an immune response in a susceptible host to prevent infection by H. pylori. The vaccines of the invention are administered in amounts readily determined by persons of ordinary skill in the art.

Thus, for adults a suitable dosage will be in the range of 10 pg to 10 g, preferably 10 ug to 100 mg, for example 50 ,ug to 50 mg. A suitable dosage for adults will also be in the range of 5 pg to 500 mg. Similar dosage ranges will be applicable for children.

The amount of adjuvant employed will depend on the type of adjuvant used. For example, when the mucosal adjuvant is cholera toxin, it is suitably used in an amount of 5 ptg to 50 llg, for example 10 pg to 35 pg. When used in the form of microcapsules, the amount used will depend on the amount employed in the matrix of the microcapsule to achieve the desired dosage. The determination of this amount is within the skill of a person of ordinary skill in the art.

Those skilled in the art will recognize that the optimal dose may be more or less depending upon the patient's body weight, disease, the route of administration, and other factors. Those skilled in the art will also recognize that appropriate dosage levels can be obtained based on results with known oral vaccines such as, for example, a vaccine based on an E. coli lysate (6 mg dose daily up to total of 540 mg) and with an enterotoxigenic E. coli purified antigen (4 doses of 1 mg) (Schulman et al., J. Urol.

150:917-921 (1993)); Boedecker et al., American Gastroenterological Assoc. 999:A-222 (1993)). The number of doses will depend upon the disease, the formulation, and efficacy data from clinical trials. Without intending any limitation as to the course of treatment, the treatment can be administered over 3 to 8 doses for a primary immunization schedule over 1 month (Boedeker, American Gastroenterological Assoc.

888:A-222 (1993)).

In a preferred embodiment, a vaccine composition of the invention can be based on a killed whole E. coli preparation with an immunogenic fragment of an H. pylori protein of the invention expressed on its surface or it can be based on an E. coli lysate, wherein the killed E. coli acts as a carrier or an adjuvant.

It will be apparent to those skilled in the art that some of the vaccine compositions of the invention are useful only for preventing H. pylori infection, some are useful only for treating H. pylori infection, and some are useful for both preventing and treating H. pylori infection. In a preferred embodiment, the vaccine composition of the invention provides protection against H. pylori infection by stimulating humoral and/or cell-mediated immunity against H. pylori. It should be understood that amelioration of any of the symptoms of H. pylori infection is a desirable clinical goal, including a lessening of the dosage of medication used to treat H. pylori-caused disease, or an increase in the production of antibodies in the serum or mucous of patients.

Vll. Antibodies Reactive With H. oviori Po1vpeptides The invention also includes antibodies specifically reactive with the subject H.

pylori polypeptide. Anti-protein/anti-peptide antisera or monoclonal antibodies can be made by standard protocols (See. for example, Antibodies: A Laboratory Manual ed. by Harlow and Lane (Cold Spring Harbor Press: 1988)). A mammal such as a mouse, a hamster or rabbit can be immunized with an immunogenic form of the peptide.

Techniques for conferring immunogenicity on a protein or peptide include conjugation to carriers or other techniques well known in the art. An immunogenic portion of the subject H. pylori polypeptide can be administered in the presence of adjuvant. The progress of immunization can be monitored by detection of antibody titers in plasma or serum. Standard ELISA or other immunoassays can be used with the immunogen as antigen to assess the levels of antibodies In a preferred embodiment, the subject antibodies are immunospecific for antigenic determinants of the H. pylori polypeptides of the invention, e.g. antigenic determinants of a polypeptide of the invention contained in the Sequence Listing, or a closely related human or non-human mammalian homolog (e.g., 90% homologous, more preferably at least 95% homologous). In yet a further preferred embodiment of the invention, the anti-H. pylori antibodies do not substantially cross react (i.e., react specifically) with a protein which is for example, less than 80% percent homologous to a sequence of the invention contained in the Sequence Listing. By "not substantially cross react", it is meant that the antibody has a binding affinity for a non-homologous protein which is less than 10 percent, more preferably less than 5 percent, and even more preferably less than 1 percent, of the binding affinity for a protein of the invention contained in the Sequence Listing. In a most preferred embodiment, there is no crossreactivity between bacterial and mammalian antigens.

The term antibody as used herein is intended to include fragments thereof which are also specifically reactive with H. pylori polypeptides. Antibodies can be fragmented

using conventional techniques and the fragments screened for utility in the same manner as described above for whole antibodies. For example, F(ab')2 fragments can be generated by treating antibody with pepsin. The resulting F(ab')2 fragment can be treated to reduce disulfide bridges to produce Fab' fragments. The antibody of the invention is further intended to include bispecific and chimeric molecules having an anti-H pylori portion.

Both monoclonal and polyclonal antibodies (Ab) directed against H. pylori polypeptides or H. pylori polypeptide variants, and antibody fragments such as Fab and F(ab)2, can be used to block the action of H pylori polypeptide and allow the study of the role of a particular H. pylori polypeptide of the invention in aberrant or unwanted intracellular signaling, as well as the normal cellular function of the H. pylori and by microinjection of anti-H pylori polypeptide antibodies of the present invention.

Antibodies which specifically bind H. pylori epitopes can also be used in immunohistochemical staining of tissue samples in order to evaluate the abundance and pattern of expression of H. pylori antigens. Anti H. pylori polypeptide antibodies can be used diagnostically in immuno-precipitation and immuno-blotting to detect and evaluate H. pylori levels in tissue or bodily fluid as part of a clinical testing procedure. Likewise, the ability to monitor H. pylori polypeptide levels in an individual can allow determination of the efficacy of a given treatment regimen for an individual afflicted with such a disorder. The level of an H. pylori polypeptide can be measured in cells found in bodily fluid, such as in urine samples or can be measured in tissue, such as produced by gastric biopsy. Diagnostic assays using anti-H. pylori antibodies can include, for example, immunoassays designed to aid in early diagnosis of H pylori infections. The present invention can also be used as a method of detecting antibodies contained in samples from individuals infected by this bacterium using specific H. pylori antigens.

Another application of anti-H pylori polypeptide antibodies of the invention is in the immunological screening of cDNA libraries constructed in expression vectors such as kgtl 1, Xgt18-23, XZAP, and SORT8. Messenger libraries of this type, having coding sequences inserted in the correct reading frame and orientation, can produce fusion proteins. For instance, kgtl 1 will produce fusion proteins whose amino termini consist of 13-galactosidase amino acid sequences and whose carboxy termini consist of a foreign polypeptide. Antigenic epitopes of a subject H. pylori polypeptide can then be detected with antibodies, as, for example, reacting nitrocellulose filters lifted from infected plates with anti-H. pylori polypeptide antibodies. Phage, scored by this assay, can then be isolated from the infected plate. Thus, the presence of H pylori gene

homologs can be detected and cloned from other species, and alternate isoforms (including splicing variants) can be detected and cloned.

VIII. Kits Containing Nucleic Acids, Polypeptides or Antibodies of the Invention The nucleic acid, polypeptides and antibodies of the invention can be combined with other reagents and articles to form kits. Kits for diagnostic purposes typically comprise the nucleic acid, polypeptides or antibodies in vials or other suitable vessels.

Kits typically comprise other reagents for performing hybridization reactions, polymerase chain reactions (PCR), or for reconstitution of lyophilized components, such as aqueous media, salts, buffers, and the like. Kits may also comprise reagents for sample processing such as detergents, chaotropic salts and the like. Kits may also comprise immobilization means such as particles, supports, wells, dipsticks and the like.

Kits may also comprise labeling means such as dyes, developing reagents, radioisotopes, fluorescent agents, luminescent or chemiluminescent agents, enzymes, intercalating agents and the like. With the nucleic acid and amino acid sequence information provided herein, individuals skilled in art can readily assemble kits to serve their particular purpose. Kits further can include instructions for use.

IX. Drug Screening Assays Using H. i,vThri Polypeptides By making available purified and recombinant H. pylori polypeptides, the present invention provides assays which can be used to screen for drugs which are either agonists or antagonists of the normal cellular function, in this case, of the subject H.

pylori polypeptides, or of their role in intracellular signaling. Such inhibitors or potentiators may be useful as new therapeutic agents to combat H. pylori infections in humans. A variety of assay formats will suffice and, in light of the present inventions, will be comprehended by the skilled artisan.

In many drug screening programs which test libraries of compounds and natural extracts, high throughput assays are desirable in order to maximize the number of compounds surveyed in a given period of time. Assays which are performed in cell-free systems, such as may be derived with purified or semi-purified proteins, are often preferred as "primary" screens in that they can be generated to permit rapid development and relatively easy detection of an alteration in a molecular target which is mediated by a test compound. Moreover, the effects of cellular toxicity and/or bioavailability of the test compound can be generally ignored in the in vitro system, the assay instead being focused primarily on the effect of the drug on the molecular target as may be manifest in an alteration of binding affinity with other proteins or change in enzymatic properties of the molecular target. Accordingly, in an exemplary screening assay of the present

invention, the compound of interest is contacted with an isolated and purified H. pylori polypeptide.

Screening assays can be constructed in vitro with a purified H. pylori polypeptide or fragment thereof, such as an H. pylori polypeptide having enzymatic activity, such that the activity of the polypeptide produces a detectable reaction product.

The efficacy of the compound can be assessed by generating dose response curves from data obtained using various concentrations of the test compound. Moreover, a control assay can also be performed to provide a baseline for comparison. Suitable products include those with distinctive absorption, fluorescence, or chemi-luminescence properties, for example, because detection may be easily automated. A variety of synthetic or naturally occurring compounds can be tested in the assay to identify those which inhibit or potentiate the activity of the H. pylori polypeptide. Some of these active compounds may directly, or with chemical alterations to promote membrane permeability or solubility, also inhibit or potentiate the same activity (e.g., enzymatic activity) in whole, live H. pylori cells.

This invention is further illustrated by the following examples which should not be construed as limiting. The contents of all references and published patent applications cited throughout this application are hereby incorporated by reference.

EXEMPLIFICATION I. Cloning and Sequenceing of H pylori DNA H. pylori chromosomal DNA was isolated according to a basic DNA protocol outlined in Schleif R.F. and Wensink P.C., Practical Methods in Molecular Biology, p.98, Springer-Verlag, NY., 1981, with minor modifications. Briefly, cells were pelleted, resuspended in TE (10 mM Tris, 1 mM EDTA, pH 7.6) and GES lysis buffer (5.1 M guanidium thiocyanate, 0.1 M EDTA, pH 8.0, 0.5% N-laurylsarcosine) was added. Suspension was chilled and ammonium acetate (NH4Ac) was added to final concentration of 2.0 M. DNA was extracted, first with chloroform, then with phenol- chloroform, and reextracted with chloroform. DNA was precipitated with isopropanol, washed twice with 70% EtOH, dried and resuspended in TE.

Following isolation whole genomic H. pylori DNA was nebulized (Bodenteich et al., Automated DNA Sequencing and Analysis (J.C. Venter, ed.), Academic Press, 1994) to a median size of 2000 bp. After nebulization, the DNA was concentrated and separated on a standard 1% agarose gel. Several fractions, corresponding to

approximate sizes 900-1300 bp. 1300-1700 bp, 1700-2200 bp, 2200-2700 bp, were excised from the gel and purified by the GeneClean procedure (Bio101, Inc.).

The purified DNA fragments were then blunt-ended using T4 DNA polymerase.

The healed DNA was then ligated to unique BstXI-linker adapters in 100-1000 fold molar excess. These linkers are complimentary to the BstXI-cut pMPX vectors while the overhang is not self-complimentary. Therefore, the linkers will not concatemerize nor will the cut-vector religate itself easily. The linker-adopted inserts were separated from the unincorporated linkers on a 1% agarose gel and purified using GeneClean. The linker-adopted inserts were then ligated to each of the 20 pMPX vectors to construct a series of "shotgun" subclone libraries. The vectors contain an out-of-frame lacZ gene at the cloning site which becomes in-frame in the event that an adapter-dimer is cloned, allowing these to be avoided by their blue-color.

All subsequent steps were based on the multiplex DNA sequencing protocols outlined in Church G.M. and Kieffer-Higgins S., Science 240:185-188, 1988. Only major modifications to the protocols are highlighted. Briefly, each of the 20 vectors was then transformed into DHSa competent cells (Gibco/BRL, DHSa transformation protocol). The libraries were assessed by plating onto antibiotic plates containing ampicillin, methicillin and IPTG/Xgal. The plates were incubated overnight at 370C.

Successful transformants were then used for plating of clones and pooling into the multiplex pools. The clones were picked and pooled into 40 ml growth medium cultures. The cultures were grown overnight at 370C. DNA was purified using the Qiagen Midi-prep kits and Tip-100 columns (Qiagen, Inc.). In this manner, 100 ,ug of DNA was obtained per pool. Fifteen 96-well plates of DNA were generated to obtain a 5-10 fold sequence redundancy assuming 250-300 base average read-lengths.

These purified DNA samples were then sequenced using the multiplex DNA sequencing based on chemical degradation methods (Church G.M. and Kieffer-Higgins S., Science 240:185-188, 1988) or by Sequithrem (Epicenter Technologies) dideoxy sequencing protocols. The sequencing reactions were electrophoresed and transferred onto nylon membranes by direct transfer electrophoresis from 40 cm gels (Richterich P.

and Church G.M., Methods in Enzymology 218:187-222, 1993) or by electroblotting (Church, supra). 24 samples were run per gel. 45 successful membranes were produced by chemical sequencing and 8 were produced by dideoxy sequencing. The DNA was covalently bound to the membranes by exposure to ultraviolet light, and hybridized with labeled oligonucleotides complimentary to tag sequences on the vectors (Church, supra).

The membranes were washed to rinse off non-specifically bound probe, and exposed to X-ray film to visualize individual sequence ladders. After autoradiography, the hybridized probe was removed by incubation at 650 C, and the hybridization cycle

repeated with another tag sequence until the membrane had been probed 38 times for chemical sequencing membranes and 10 times for the dideoxy sequencing membranes.

Thus, each gel produced a large number of films, each containing new sequencing information. Whenever a new blot was processed, it was initially probed for an internal standard sequence added to each of the pools.

Digital images of the films were generated using a laser-scanning densitometer (Molecular Dynamics, Sunnyvale, CA). The digitized images were processed on computer workstations (VaxStation 4000's) using the program REPLICATM (Church et al., Automated DNA Sequencing and Analysis (J.C. Venter, ed.), Academic Press, 1994).

Image processing included lane straightening, contrast adjustment to smooth out intensity differences, and resolution enhancement by iterative gaussian deconvolution.

The sequences were then automatically picked in REPLICATM and displayed for interactive proofreading before being stored in a project database. The proofreading was accomplished by a quick visual scan of the film image followed by mouse clicks on the bands of the displayed image to modify the base calls. Many of the sequence errors could be detected and corrected because multiple sequence reads covering the same portion of the genomic DNA provide adequate sequence redundancy for editing. Each sequence automatically received an identification number (corresponding to microtiter plate, probe information, and lane set number). This number serves as a permanent identifier of the sequence so it is always possible to identify the original of any particular sequence without recourse to a specialized database.

Routine assembly of H pylori sequences was done using the program FALCON (Church, Church et al., Automated DNA Sequenicng and Analysis (J.C. Venter, ed.), Academic Press, 1994). This program has proven to be fast and reliable for most sequences. The assembled contigs were displayed using a modified version of GelAssemble, developed by the Genetics Computer Group (GCG) (Devereux et al., Nucleic Acid Res. 12:387-95, 1984) that interacts with REPLICATM. This provided for an integrated editor that allows multiple sequence gel images to be instantaneously called up from the REPLICATM database and displayed to allow rapid scanning of contigs and proofreading of gel traces where discrepancies occurred between different sequence reads in the assembly.

II. Identification. cloning and expression of recombinant H. pylori DNA sequences To facilitate the cloning, expression and purification of membrane and secreted proteins from H. pylori a powerful gene expression system, the pET System (Novagen), for cloning and expression of recombinant proteins in E. coli, was selected. Also, a DNA sequence encoding a peptide tag, the His-Tag, was fused to the 3' end of DNA

sequences of interest in order to facilitate purification of the recombinant protein products. The 3' end was selected for fusion in order to avoid alteration of any 5' terminal signal sequence. The exception to the above was ppiB, a gene cloned for use as a control in the expression studies. In this study, the sequence for H. pylori ppiB contains a DNA sequence encoding a His-Tag fused to the 5' end of the full length gene, because the protein product of this gene does not contain a signal sequence and is expressed as a cytosolic protein.

PCR Amplification and cloning ofDNA sequences containing ORF'sfor membrane and secreted proteins from the J99 Strain ofHelicobacter pylori.

Sequences chosen (from the list of the DNA sequences of the invention) for cloning from the J99 strain of H pylori were prepared for amplification cloning by polymerase chain reaction (PCR). Synthetic oligonucleotide primers (Table 3) specific for the 5' and 3' ends of open reading frames (ORFs) were designed and purchased (GibcoBRL Life Technologies, Gaithersburg, MD, USA). All forward primers (specific for the 5' end of the sequence) were designed to include an NcoI cloning site at the extreme 5' terminus, except for HpSeq. 4821082 where NdeI was used. These primers were designed to permit initiation of protein translation at a methionine residue followed by a valine residue and the coding sequence for the remainder of the native H. pylori DNA sequence. An exception is H. pylori sequence 4821082 where the initiator methionine is immediately followed by the remainder of the native H. pylori DNA sequence. All reverse primers (specific for the 3' end of any H. pylori ORF) included a EcoRI site at the extreme 5' terminus to permit cloning of each H. pylori sequence into the reading frame of the pET-28b. The pET-28b vector provides sequence encoding an additional 20 carboxy-terminal amino acids (only 19 amino acids in lIp Seq. 26380318 and HpSeq. 14640637) including six histidine residues (at the extreme C-terminus), which comprise the His-Tag. An exception to the above, as noted earlier, is the vector construction for the ppiB gene. A synthetic oligonucleotide primer specific for the 5' end of ppiB gene encoded a BamHI site at its extreme 5' terminus and the primer for the 3' end of the ppiB gene encoded a XhoI site at its extreme 5' terminus.

TABLE 3 Oligonucleotide primers used for PCR amplification of H. pylori DNA sequences Outer membrane Forward primer 5' to 3' Reverse Primer 5' to 3' Proteins Protein 16225006 5'-TATACCATGGTGGG 5'- CGCTAA-3' (SEQ ID ATGAATTCGAGTAAG NO:147) GATTTTTG-3' (SEQ ID NO:148) Protein 26054702 5'- 5'- TTAACCATGGTGAAA TAGAATTCGCATAAC AGCGATA-3' (SEQ ID GATCAATC-3' (SEQ ID NO:149) NO:150) Protein 7116626 5 5'- ATATCCATGGTGAGT ATGAATTCAATTTTT TTGATGA-3' (SEQ ID TATTTTGCCA-3' (SEQ NO:151) ID NO:152) Protein 29479681 5 5'- AATTCCATGGTGGGG ATGAATTCTCGATAG GCTATG-3' (SEQ ID CCAAAATC-3' (SEQ ID NO:153) NO:154) Protein 14640637 5 5'- AATTCCATGGTGCAT AAGAATTCTCTAGCA AACTTCCATT-3' (SEQ TCCAAATGGA-3' (SEQ ID NO:155) ID NO:156) Periplasmic/ Secreted Proteins Protein 30100332 5'-ATTTCCATGGTCATG 5'- TCTCATATT-3' (SEQ ID ATGAATTCCATCTTT NO:157) TATTCCAC-3' (SEQ ID NO:158) Protein 4721061 5'-AACCATGGTGATTT 5'- TAAGCATTGAAAG-3' AAGAATTCCACTCA (SEQ ID NO:159) AAATTTTTTAACAG-3' (SEQ ID NO: 160) Other Surface Proteins Protein 4821082 5'-GATCATCCATATGTT 5'- ATCTTCTAAT-3' (SEQ TGAATTCAACCATTT ID NO:161) TAACCCTG-3' (SEQ ID NO:162)

Protein 978477 5'-TATACCATGGTGAA 5'- ATTTTTTCTTTTA-3' AGAATTCAATTGCG (SEQ ID NO:163) TCTTGTAAAAG-3' (SEQ ID NO:164) Inner Membrane Protein Protein 26380318 5'-TATACCATGGTGAT 5'-ATGAATTCCCACTT GGACAAACTC-3' (SEQ GGGGCGATA-3' (SEQ IDNO:165) IDNO:166) Cytoplasmic Protein ppi 5'-TTATGGATCCAAAC 5'-TATCTCGAGTTATA CAATTAAAACT-3' (SEQ GAGAAGGGC-3' (SEQ IDNO:167) IDNO:168) Genomic DNA prepared from the J99 strain of H pylori (ATCC #55679; deposited by Genome Therapeutics Corporation, 100 Beaver Street, Waltham, MA 02154) was used as the source of template DNA for PCR amplification reactions (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994). To amplify a DNA sequence containing an H. pylori ORF, genomic DNA (50 nanograms) was introduced into a reaction vial containing 2 mM MgC12, 1 micromolar synthetic oligonucleotide primers (forward and reverse primers) complementary to and flanking a defined H. pylori ORF, 0.2 mM of each deoxynucleotide triphosphate; dATP, dGTP, dCTP, dTTP and 2.5 units of heat stable DNA polymerase (Amplitaq, Roche Molecular Systems. Inc., Branchburg, NJ, USA) in a final volume of 100 microliters. The following thermal cycling conditions were used to obtain amplified DNA products for each ORF using a Perkin Elmer Cetus/ GeneAmp PCR System 9600 thermal cycler: Protein 26054702, Protein 7116626, Protein 29479681, Protein 30100332, and Protein 4821082; Denaturation at 940C for 2 min, 2 cycles at 940C for 15 sec, 300C for 15 sec and 720C for 1.5 min 23 cycles at 940C for 15 sec, 550C for 15 sec and 720C for 1.5 min Reactions were concluded at 720C for 6 minutes.

Protein 16225006; Denaturation at 940C for 2 min.

25 cycles at 950C for 15 sec, 550C for 15 sec and 720C for 1.5 min Reaction was concluded at 720C for 6 minutes.

Protein 4721061; Denaturation at 940C for 2 min, 2 cycles at 940C for 15 sec, 360C for 15 sec and 720C for 1.5 min 23 cycles at 940C for 15 sec, 600C for 15 sec and 720C for 1.5 min Reactions were concluded at 720C for 6 minutes.

Protein 26380318; Denaturation at 940C for 2 min, 2 cycles at 940C for 15 sec, 380C for 15 sec and 720C for 1.5 min 23 cycles at 940C for 15 sec 620C for 15 sec and 720C for 1.5 min Reactions were concluded at 720C for 6 minutes.

Protein 14640637; Denaturation at 940C for 2 min, 2 cycles at 940C for 15 sec, 330C for 15 sec and 720C for 1.5 min 30 cycles at 940C for 15 sec, 550C for 15 sec and 720C for 1.5 min Reactions were concluded at 720C for 6 minutes.

Conditions for amplification of H. pylori ppiB; Denaturation at 940C for 2 min 2 cycles at 940C for 15 sec, 320C for 15 sec and 720C for 1.5 min 25 cycles at 940C for 15 sec, 560C for 15 sec and 720C for 1.5 min Reactions were concluded at 720C for 6 minutes Upon completion of thermal cycling reactions, each sample of amplified DNA was washed and purified using the Qiaquick Spin PCR purification kit (Qiagen, Gaithersburg, MD, USA). All amplified DNA samples were subjected to digestion with the restriction endonucleases, NcoI and EcoRI (New England BioLabs, Beverly, MA, USA), or in the case of HpSeq. 4821082 (SEQ ID NO: 1309), with NdeI and EcoRI (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994). DNA samples were then subjected to electrophoresis on 1.0 % NuSeive (FMC BioProducts, Rockland, ME USA) agarose gels. DNA was visualized by exposure to ethidium bromide and long wave uv irradiation. DNA contained in slices isolated from the agarose gel was purified using the Bio 101 GeneClean Kit protocol (Bio 101 Vista, CA, USA).

Cloning ofH. pylori DNA sequences into the pET-28b prokaryotic expression vector.

The pET-28b vector was prepared for cloning by digestion with NcoI and EcoRI, or in the case of H. pylori protein 4821082 with NdeI and EcoRI (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds. 1994). In the case of cloning ppiB, the pET-28a vector, which encodes a His-Tag that can be fused to the 5' end of an inserted gene, was used and the cloning site prepared for cloning with the ppiB gene by digestion with BamHI and XhoI restriction endonucleases.

Following digestion, DNA inserts were cloned (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994) into the previously digested pET-28b expression vector, except for the amplified insert for ppiB, which was cloned into the pET-28a expression vector. Products of the ligation reaction were then used to transform the BL21 strain of E. coli (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994) as described below.

Transformation of competent bacteria with recombinant plasmids Competent bacteria, E coli strain BL21 or E. coli strain BL21(DE3), were transformed with recombinant pET expression plasmids carrying the cloned H. pylori sequences according to standard methods (Current Protocols in Molecular, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994). Briefly, 1 microliter of ligation reaction was mixed with 50 microliters of electrocompetent cells and subjected to a high voltage pulse, after which, samples were incubated in 0.45 milliliters SOC medium (0.5% yeast extract, 2.0 % tryptone, 10 mM NaCI, 2.5 mM KC1, 10 mM MgC12, 10 mM MgS04 and 20, mM glucose) at 370C with shaking for 1 hour. Samples were then spread on LB agar plates containing 25 microgram/ml kanamycin sulfate for growth overnight.

Transformed colonies of BL2 1 were then picked and analyzed to evaluate cloned inserts as described below.

Identification of recombinant pET expression plasmids carrying H. pylori sequences Individual BL21 clones transformed with recombinant pET-28b-H.pylori ORFs were analyzed by PCR amplification of the cloned inserts using the same forward and reverse primers, specific for each H. pylori sequence, that were used in the original PCR amplification cloning reactions. Successful amplification verified the integration of the H. pylori sequences in the expression vector (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994).

Isolation and Preparation ofplasmid DNA from BL21 transformants Individual clones of recombinant pET-28b vectors carrying properly cloned H.

pylori ORFs were picked and incubated in 5 mls of LB broth plus 25 microgram/mi kanamycin sulfate overnight. The following day plasmid DNA was isolated and purified using the Qiagen plasmid purification protocol (Qiagen Inc., Chatsworth, CA, USA).

Expression ofrecombinant H. pylori sequences in E. coli The pET vector can be propagated in any E. coli K-12 strain e.g. HMS 174, HB 101, JM 109, Dill, etc. for the purpose of cloning or plasmid preparation. Hosts for expression include E. coli strains containing a chromosomal copy of the gene for T7 RNA polymerase. These hosts are lysogens of bacteriophage DE3, a lambda derivative that carries the lacI gene, the lacUV5 promoter and the gene for T7 RNA polymerase.

T7 RNA polymerase is induced by addition of isopropyl-B-D-thiogalactoside (IPTG), and the T7 RNA polymerase transcribes any target plasmid, such as pET-28b, carrying a T7 promoter and a gene of interest. Strains used include: BL21(DE3) (Studier, F.W., Rosenberg, A.H., Dunn, J.J., and Dubendorff, J.W. (1990) Meth. Enzymol. 185, 60-89).

To express recombinant H. pylori sequences, 50 nanograms of plasmid DNA isolated as described above was used to transform competent BL21(DE3) bacteria as described above (provided by Novagen as part of the pET expression system kit). The lacZ gene (beta-galactosidase) was expressed in the pET-System as described for the H.

pylori recombinant constructions. Transformed cells were cultured in SOC medium for 1 hour, and the culture was then plated on LB plates containing 25 micrograms/ml kanamycin sulfate. The following day, bacterial colonies were pooled and grown in LB medium containing kanamycin sulfate (25 micrograms/ml) to an optical density at 600 nM of 0.5 to 1.0 O.D. units, at which point, 1 millimolar IPTG was added to the culture for 3 hours to induce gene expression of the H. pylori recombinant DNA constructions.

After induction of gene expression with IPTG, bacteria were pelleted by centrifugation in a Sorvall RC-3B centrifuge at 3500 x g for 15 minutes at 40C. Pellets were resuspended in 50 milliliters of cold 10 mM Tris-HCl, pH 8.0, 0.1 M NaCl and 0.1 mM EDTA (STE buffer). Cells were then centrifuged at 2000 x g for 20 min at 40C.

Wet pellets were weighed and frozen at -800C until ready for protein purification.

III. Purification of recombinant Proteins from E. coli Analytical Methods The concentrations of purified protein preparations were quantified spectrophotometrically using absorbance coefficients calculated from amino acid content (Perkins, S.J. 1986 Eur. J. Biochem. 157, 169-180). Protein concentrations were also measured by the method of Bradford, M.M. (1976) Anal. Biochem. 72, 248-254, and Lowry, O.H., Rosebrough, N., Farr, A.L. & Randall, R.J. (1951) J. Biol. Chem. 193, pages 265-275, using bovine serum albumin as a standard.

SDS-polyacrylamide gels (12% or 4.0 to 25 % acrylamide gradient gels) were purchased from BioRad (Hercules, CA, USA), and stained with Coomassie blue.

Molecular weight markers included rabbit skeletal muscle myosin (200 kDa), E. coli (- galactosidase ( l l 6 kDa), rabbit muscle phosphorylase B (97.4 kDa), bovine serum albumin (66.2 kDa), ovalbumin (45 kDa), bovine carbonic anhydrase (31 kDa), soybean trypsin inhibitor (21.5 kDa), egg white lysozyme (14.4 kDa) and bovine aprotinin (6.5 kDa).

1. Purification of soluble proteins All steps were carried out at 40C. Frozen cells were thawed, resuspended in 5 volumes of lysis buffer (20 mM Tris, pH 7.9, 0.5 M NaCl, 5 mM imidazole with 10% glycerol, 0.1 % 2-mercaptoethanol, 200 Ag/ ml lysozyme, 1 mM phenylmethylsulfonyl fluoride (PMSF), and 10 ug/ml each of leupeptin, aprotinin, pepstatin, L-l-chloro-3-[4- tosylamido]-7-amino-2-heptanone (TLCK), L- 1 -chloro-3 - [4-tosylamido]-4-phenyl-2- butanone (TPCK), and soybean trypsin inhibitor, and ruptured by several passages through a small volume microfluidizer (Model M-1 lOS, Microfluidics International Corporation, Newton, MA). The resultant homogenate was made 0.1 % Brij 35, and centrifuged at 100,000 x g for 1 hour to yield a clear supernatant (crude extract).

Following filtration through a 0.8 Hm Supor filter (Gelman Sciences, FRG) the crude extract was loaded directly onto a Ni2+- nitrilotriacetate-agarose (NTA) with a 5 milliliter bed volume (Hochuli, E., Dbeli, H., and Schacheer, A. (1987) J.

Chromatography 411, 177-184) pre-equilibrated in lysis buffer containing 10 % glycerol, 0.1 % Brij 35 and 1 mM PMSF. The column was washed with 250 ml (50 bed volumes) of lysis buffer containing 10 % glycerol, 0.1 % Brij 35, and was eluted with sequential steps of lysis buffer containing 10 % glycerol, 0.05 % Brij 35, 1 mM PMSF, and 20, 100, 200, and 500 mM imidazole in succession. Fractions were monitored by absorbance at OD280 nm, and peak fractions were analyzed by SDS-PAGE. Fractions containing the recombinant protein eluted at 100 mM imidazole.

Recombinant protein 14640637 and proteins, beta-galactosidase (lacZ) andpeptidyl- prolyl cis-trans isomerase (ppiB) Fractions containing the recombinant proteins from the Ni2+-NTA-agarose columns were pooled and then concentrated to approximately 5 ml by centrifugal filtration (Centriprep-lO, Amicon, MA), and loaded directly onto a 180-ml column (1.6 X 91 cm) of Sephacryl S-100 HR gel filtration medium equilibrated in Buffer A (10 mM Hepes, pH 7.5, 150 mM NaCl, 0.1 mM EGTA) and run in Buffer A at 18 ml/h.

Fractions containing the recombinant protein were identified by absorbance at 280 nm and analyzed by SDS-PAGE. Fractions were pooled and concentrated by centrifugal filtration.

Recombinant protein 7116626 Fractions containing the recombinant protein from the Ni2+ -NTA-agarose column were pooled and dialyzed overnight against 1 liter of dialysis buffer (10 mM MOPS, pH 6.5, 50 mM NaCI, 0.1 mM EGTA, 0.02% Brij 35 and 1 mM PMSF). In the morning, a fine white precipitate was removed by centrifugation and the resulting supernatant was loaded onto an 8 ml (8 x 75 mm) MonoS high performance liquid chromatography column (Pharmacia Biotechnology, Inc., Piscataway, NJ, USA) equilibrated in buffer B (10 mM MOPS, pH 6.5, 0.1 mM EGTA) containing 50 mM NaCl. The column was washed with 10 bed volumes of buffer B containing 50 mM NaCl, and developed with a 50-ml linear gradient of increasing NaCl (50 to 500 mM).

Recombinant protein 7116626 eluted as a sharp peak at 300 mM NaCl.

2. Purification of insoluble proteins from inclusion bodies The following steps were carried out at 40C. Cell pellets were resuspended in lysis buffer with 10% glycerol 200 clog/ ml lysozyme, 5 mM EDTA, 1mM PMSF and 0.1 % -mercaptoethanol. After passage through the cell disrupter, the resulting homogenate was made 0.2 % deoxycholate. stirred 10 minutes, then centrifuged at 20,000 x g, for 30 min. The pellets were washed with lysis buffer containing 10 % glycerol, 10 mM EDTA, 1% Triton X-100, 1 mM PMSF and 0.1% -mercaptoethanol, followed by several washes with lysis buffer containing 1 M urea, 1 mM PMSF and 0.1 % 2- mercaptoethanol. The resulting white pellet was composed primarily of inclusion bodies, free of unbroken cells and membranous materials..

Recombinant proteins 26054702, 16225006, 30100332, 4721061 The following steps were carried out at room temperature. Purified inclusion bodies were dissolved in 20 ml 8.0 M urea in lysis buffer with 1 mM PMSF and 0.1 % 2-mercaptoethanol, and incubated at room temperature for 1 hour. Materials that did not dissolve were removed by centrifugation. The clear supernatant was filtered, then loaded onto a Ni2+ -NTA agarose column pre-equilibrated in 8.0 M urea in Lysis Buffer. The column was washed with 250 ml (50 bed volumes) of lysis buffer

containing 8 M urea, 1.0 mM PMSF and 0.1 % 2-mercaptoethanol, and developed with sequential steps of lysis buffer containing 8M urea, 1 mM PMSF, 0.1 % 2- mercaptoethanol and 20, 100, 200, and 500 mM imidazole in succession. Fractions were monitored by absorbance at OD280 rin and peak fractions were analyzed by SDS- PAGE. Fractions containing the recombinant protein eluted at 100 mM imidazole.

Recombinant proteins 29479681, 26380318 The pellet containing the inclusion bodies was solubilized in buffer B containing 8 M urea, 1 mM PMSF and 0.1 % 2-mercaptoethanol, and incubated for 1 hour at room temperature. Insoluble materials were removed by centrifugation at 20,000 x g for 30 min, and the cleared supernatant was loaded onto a 15 ml ( 1.6 x 7.5 cm) SP-Sepharose column pre-equilibrated in buffer B, 6 M urea, 1 mM PMSF, 0.1 % 2-mercaptoethanol.

After washing the column with 10 bed volumes, the column was developed with a linear gradient from 0 to 500 mM NaCl.

Dialysis and concentration of protein samples Urea was removed slowly from the protein samples by dialysis against Tris- buffered saline (TBS; 10 mM Tris pH 8.0, 150 mM NaCI) containing 0.5 % deoxycholate (DOC) with sequential reduction in urea concentration as follows; 6M, 4M, 3M, 2M, 1M, 0.5 M and finally TBS without any urea. Each dialysis step was conducted for a minimum of 4 hours at room temperature.

After dialysis, samples were concentrated by pressure filtration using Amicon stirred-cells. Protein concentrations were measured using the methods of Perkins (1986 Eur. J. Biochem. 157, 169-180), Bradford ((1976) Anal. Biochem. 72, 248-254) and Lowry ((1951) J. Biol. Chem. 193, pages 265-275).

The recombinant proteins purified by the methods described above are summarized in Table 4 below.

TABLE 4 J99 Homolog Gene Bacterial cell Method of Relative - - Final Composit Sequence identified symbol fraction used to purification MW on concentratio I ionof Identifier by Blast of purify SDS- n of purified buffer Homolog recombinant PAGE gel protein proteins Outer Membrane Proteins 16225006 P28635 YEAC Inclusion bodies His-Tag 18 kDa 5 mg/ml B 26054702 P15929 figH Inclusion bodies His-Tag 37 kDa 1.18 mg/ml B

as dry pellet 7116626 P26093 e(P4) Soluble fraction His-Tag 29 kDa 0.8 mg/ml A 1.85mg/ml C 29479681 P13036 fecA Inclusions SP- 23 kDa 2.36 mg/mI B bodies Sepharose 0.5 mg ml B as dry pellet 14640637 P16665 TPF1 Soluble fraction His-Tag 17 kDa 2.4 mg/ml A gel filtration S100 HR Periplasmic/Secreted Protein 3010032 P23847 dppA Inclusion bodies His-Tag II kDa 2.88 mg/ml B 4721061 P36175 CCP Inclusion bodies His-Tag 38 kDa 2.8 mg/ml B Other Surface Proteins 4821082 P08089 M Inclusion bodies His-Tag 20 kDa 1.16 mg/ml B protein 978477 L28919 FBP54 Inclusion bodies SP- 44 kDa 2.56 mg/ml B Sepharose 0.3 mg/ml B Inner Membrane Proteins 26380318 P15933 fliG Inclusion bodies SP- 11 kDa 22 mg/ml B Sepharose Control Proteins with His-Tag P00722 lacZ Soluble fraction His-Tag 116 kDa 10 mg/ml A gel filtration S200 HR ppiB Soluble fraction His-Tag 21 kDa 4.4 mg/ml A gel filtration S100 HR Buffer composition 5: A=10 mM Hepes pH 7.5, 150 mM NaCI, 0.1 mM EGTA B 10 mM Tris pH 8.0, 150 mM NaCI, 0.5 % DOC C= 10 mM MOPS pH 6.5, 300 mM NaCl, 0.1 EGTA

IV. Analysis of H pylori proteins as Vaccine candidates To investigate the immunomodulatory effect of H pylori proteins, a mouse pylori model was used. This model mimics the human H. pylori infection in many respects. The focus is on the effect of oral immunization in H. pylori infected animals in order to test the concept of therapeutic oral immunotherapy.

Animals Female SPF BALB/c mice were purchased from Bomholt Breeding center (Denmark). They were kept in ordinary makrolon cages with free supply of water and food. The animals were 4-6 weeks old at arrival.

Infection After a minimum of one week of acclimatization, the animals were infected with a type 2 strain (VacA negative) of H pylori (strain 244, originally isolated from an ulcer patient). In our hands, this strain has earlier proven to be a good colonizer of the mouse stomach. The bacteria were grown overnight in Brucella broth supplemented with 10 % fetal calf serum, at 370C in a microaerophilic atmosphere (10% CO2, 5%O2). The animals were given an oral dose of omeprazole (400 umol/kg) and 3-5 h after this an oral inoculation of H. pylori in broth (approximately 108 cfu/animal). Positive take of the infection was checked in some animals 2-3 weeks after the inoculation.

Antigens Recombinant H. pylori antigens were chosen based on their association with externally exposed H. pylori cell membrane. These antigens were selected from the following groups: (1.) Outer Membrane Proteins; (2.) Periplastic/Secreted proteins; (3.) Outer Surface proteins; and (4.) Inner Membrane proteins. All recombinant proteins were constructed with a hexa-HIS tag for purification reasons and the non-Helicobacter pylori control protein (b-galactosidase from E. coli; LacZ), was constructed in the same way.

All antigens were given in a soluble form, i.e. dissolved in either a HEPES buffer or in a buffer containing 0.5% Deoxycholate (DOC).

The antigens are listed in Table 5 below.

Table 5 Helicobacter pylori proteins Outer membrane Proteins Protein 7116626 Protein 4721061

Protein 16225006 Protein 29479681 Protein 14640637 Periplasmic/Secreted Proteins Protein 30100332 Other cell envelope proteins Protein 4821082 Flagella-associated proteins Protein 26380318 Control proteins b-galactosidase (LacZ) Immunizations Ten animals in each group were immunized 4 times over a 34 day period (day 1, 15, 25 and 35). Purified antigens in solution or suspension were given at a dose of 100 mg/mouse. As an adjuvant, the animals were also given 10 pg/mouse of Cholera toxin (CT) with each immunization. Omeprazole (400 mmol/kg) was given orally to the animals 3-5 h prior to immunization as a way of protecting the antigens from acid degradation. Infected control animals received HEPES buffer + CT or DOC buffer + CT. Animals were sacrificed 2-4 weeks after final immunization. A general outline of the study is shown in Table 6 below.

Table 6 Studv outline, therapeutic immunization: Mice were all infected with H. pylori strain Ah244 at day 30.

Substance Mouse strain Dose/mouse Dates for n=10 dosing 1. Controls, PBS Balb/c 0.3 ml 0, 14, 24, 34 2. Cholera toxin, 10 pg Balb/c 0.3 ml 0, 14, 24, 34 3. Protein 16225006, 100 ug + CT 10 llgBalb/c 0.3 ml 0, 14, 24, 34 4. Protein 26054702, 100 ug + CT 10 ,ug Balb/c 0.3 ml 0, 14, 24, 34

5. Protein 26380318, 100 ug+CT 10 llgBalb/c 0.3 ml 0, 14, 24, 34 6. Protein 29479681, 100 ug + CT 10 µg Balb/c 0.3 ml 0, 14, 24, 34 7. Protein 30100332, 100 ug + CT 10 pgBalb/c 0.3 ml 0, 14, 24, 34 8. Protein 4721061, 100 µg + CT 10 µg Balb/c 0.3 ml 0, 14, 24, 34 9. Protein 4821082, 100 ug + CT 10 ug Balb/c 0.3 ml 0, 14, 24, 34 <BR> <BR> <BR> <BR> <BR> <BR> 10. Protein 7116626, 100 llg + CT 10 Ag Balb/c 0.3 ml 0, 14, 24, 34 11.Protein 14640637, 100 ug + CT 10 ug Balb/c 0.3 ml 0, 14, 24, 34 Analysis of infection Mucosal infection: The mice were sacrificed by CO2 and cervical dislocation.

The abdomen was opened and the stomach removed. After cutting the stomach along the greater curvature, it was rinsed in saline. The mucosa from the antrum and corpus of an area of 25mm was scraped separately with a surgical scalpel. The mucosa scraping was suspended in Brucella broth and plated onto Blood Skirrow selective plates. The plates were incubated under microaerophilic conditions for 3-5 days and the number of colonies was counted. The identity of H pylori was ascertained by urease and catalase test and by direct microscopy or Gram staining.

The urease test was performed essentially as follows. The reagent, Urea Agar Base Concentrate, was purchased from DIFCO Laboratories, Detroit, MI (Catalog It 0284-61-3). Urea agar base concentrate was diluted 1:10 with water. 1 ml of if the diluted concentrate was mixed with 100-200 ml of actively growing H. pylori cells.

Color change to magenta indicated that cells were urease positive.

The catalase test was performed essentially as follows. The reagent, N,N,N',N'- Tetramethyl-p-Phenylenediamine, was purchased from Sigma, St. Louis, MO (Catalog It T3 134). A solution of the regent (1% w/v in water) was prepared. H. pylori cells were swabbed onto Whatman filter paper and overlaid with the 1% solution. Color change to dark blue indicated that the cells were catalase positive.

Serum antibodies: From all mice serum was prepared from blood drawn by heart puncture. Serum antibodies were identified by regular ELISA techniques, where the specific antigens of Helicobacter pylori were plated.

Mucosal antibodies: Gentle scrapings of a defined part of the corpus and of 4 cm of duodenum were performed in 50% of the mice in order to detect the presence of

antibodies in the mucous. The antibody titers were determined by regular ELISA technique as for serum antibodies.

Statistical analysis: Wilcoxon-Mann-Whitney sign rank test was used for determination of significant effects of the antigens on Helicobacter pylori colonization.

P<0.05 was considered significant. Because the antrum is the major colonization site for Helicobacter most emphasis was put upon changes in the antral colonization.

Results Antibodies in sera: All antigens tested given together with CT gave rise to a measurable specific titer in serum. The highest responses were seen with Protein 7116626, Protein 4721061, Protein 26380318, Protein 14640637 and Protein 4821082 (see Figure 1).

Antibodies in mucus: In the mucus scrapings, specific antibodies against all antigens tested were seen. By far the strongest response was seen with Protein 30100332, followed by Protein 14640637, and Protein 26380318 (see Figure 2).

Therapeutic immunization effects: All control animals (BALB/c mice) were well colonized with H. pylori (strain AH244) in both antrum and corpus of the stomach. Of the antigens tested 3 proteins (Protein 4721061, Protein 4821082, and Protein 14640637) gave a good and significant reduction and/or eradication of the H. pylori infection. The degree of colonization of the antrum was lower following immunization with Protein 7116626 and Protein 26380318 compared to control. The effect of Proteins 16225006, 29479681, and 30100332 did not differ from control. The control protein lacZ, i.e. the non-H. pylori protein, had no eradication effect and in fact had higher Helicobacter colonization compared to the HEPES + CT control. All data are shown in Figures 3 and 4 for proteins dissolved in HEPES and DOC respectively. Data is shown as geometric mean values. n=8-10 Wilcoxon-Mann-Whitney sign rank test * = p<0.05; x/10 = number of mice showing eradication of H. pylori over the total number of mice examined.

The data presented indicate that all of the H. pylori associated proteins included in this study, when used as oral immunogens in conjunction with the oral adjuvant CT, resulted in stimulation of an immune response as measured by specific serum and mucosal antibodies. A majority of the proteins led to a reduction, and in some cases complete clearance of the colonization of H pylori in this animal model. It should be noted that the reduction or clearance was due to heterologous protection rather than homologous protection (the polypeptides were based on the H. pylori J99 strain

sequence and used in the therapeutic immunization studies against a different (AH244) challenge strain, indicating the vaccine potential against a wide variety of H pylori strains.

The highest colonization in the antrum was seen in animals treated with the non- Helicobacter protein LacZ, indicating that the effects seen with the Helicobacter pylori antigens were specific.

Taken together these data strongly support the use of these H. pylori proteins in a pharmaceutical formulation for the use in humans to treat and/or prevent H. pylori infections.

V. Sequence Variance Analysis of genes in Helicobacter pylori strains Four genes were cloned and sequenced from several strains of H. pylori to compare the DNA and deduced amino acid sequences. This information was used to determine the sequence variation between the H. pylori strain, J99, and other H. pylori strains isolated from human patients.

Preparation of Chromosomal DNA.

Cultures of H pylori strains (as listed in Table 9) were grown in BLBB (1% Tryptone, 1% Peptamin 0.1% Glucose, 0.2% Yeast Extract 0.5% Sodium Chloride, 5% Fetal Bovine Serum) to an OD600 of 0.2. Cells were centrifuged in a Sorvall RC-3B at 3500 x g at 40C for 15 minutes and the pellet resuspended in 0.95 mls of 10 mM Tris- HCI, 0.1 mM EDTA (TE). Lysozyme was added to a final concentration of lmg/ml along with, SDS to 1% and RNAse A + T1 to 0.5mg/ml and 5 units/ml respectively, and incubated at 370C for one hour. Proteinase K was then added to a final concentration of 0.4mg/ml and the sample was incubated at 55 C for more than one hour. NaCl was added to the sample to a concentration of 0.65 M, mixed carefully, and 0.15 ml of 10% CTAB in 0.7M NaCL (final is 1% CTAB/70mM NaCL) was added followed by incubation at 650C for 20 minutes. At this point, the samples were extracted with chloroform:isoamyl alcohol, extracted with phenol, and extracted again with chloroform:isoamyl alcohol. DNA was precipitated with either EtOH (1.5 x volumes) or isopropanol (0.6 x volumes) at -700C for 1 0minutes, washed in 70% EtOH and resuspended in TE.

PCR Amplification and cloning.

Genomic DNA prepared from twelve strains of Helicobacter pylori was used as the source of template DNA for PCR amplification reactions (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., editors, 1994). To

amplify a DNA sequence containing an H. pylori ORF, genomic DNA (10 nanograms) was introduced into a reaction vial containing 2 mM MgCl2, 1 micromolar synthetic oligonucleotide primers (forward and reverse primers, see Table 7) complementary to and flanking a defined H. pylori ORF, 0.2 mM of each deoxynucleotide triphosphate; dATP, dGTP, dCTP, dTTP and 0.5 units of heat stable DNA polymerase (Amplitaq, Roche Molecular Systems, Inc., Branchburg, NJ, USA) in a final volume of 20 microliters in duplicate reactions.

Table 7 Oligonucleotide primers used for PCR amplification of H pylori DNA sequences. Outer membrane Forward primer 5' to 3' Reverse Primer 5' to 3' Proteins Protein 26054702 (for 5 5'- strains AH4, AH15, TTAACCATGGTGAAA TAGAATTCGCCTCTA AH61, 5294, 5640, AGCGATA-3' (SEQ ID AAACTTTAG-3' (SEQ AH18, and AH244) NO: 169) IDNO:170) Protein 26054702 5'- 5'- (for strains AH5, 5155, TTAACCATGGTGAAA TAGAATTCGCATAAC 7958, AH24,and J99) AGCGATA-3' (SEQ ID GATCAATC-3' (SEQ ID NO:171) NO:172) Protein 7116626 5'- 5'- ATATCCATGGTGAGT ATGAATTCAATTTTT TTGATGA-3' (SEQ ID TATTTTGCCA-3' (SEQ NO:173) ID NO:174) Protein 29479681 5'- 5'- AATTCCATGGCTATC ATGAATTCGCCAAAA CAAATCCG-3' (SEQ ID TCGTAGTATT-3' (SEQ NO:175) ID NO:176) Protein 346 5' 5'- GATACCATGGAATTT TGAATTCGAAAAAGT ATGAAAAAG-3' (SEQ GTAGTTATAC-3' (SEQ IDNO:177) IDNO:178) The following thermal cycling conditions were used to obtain amplified DNA products for each ORF using a Perkin Elmer Cetus/ GeneAmp PCR System 9600 thermal cycler:

Protein 7116626 and Protein 346; Denaturation at 940C for 2 min, 2 cycles at 940C for 15 sec, 30"C for 15 sec and 720C for 1.5 min 23 cycles at 940C for 15 sec, 550C for 15 sec and 720C for 1.5 min Reactions were concluded at 720C for 6 minutes.

Protein 26054702 for strains AH5, 5155, 7958, AH24,and J99; Denaturation at 940C for 2 min, 2 cycles at 940C for 15 sec, 30"C for 15 sec and 72"C for 1.5 min 25 cycles at 940C for 15 sec, 55"C for 15 sec and 72"C for 1.5 min Reaction was concluded at 720C for 6 minutes.

Protein 26054702 and Protein 294796813 for strains AH4, AH15, AH61, 5294, 5640, AH18, and Hp244; Denaturation at 940C for 2 min, 2 cycles at 940C for 15 sec, 30"C for 20 sec and 720C for 2 min 25 cycles at 940C for 15 sec, 55"C for 20 sec and 720C for 2 min Reactions were concluded at 720C for 8 minutes.

Upon completion of thermal cycling reactions, each pair of samples were combined and used directly for cloning into the pCR cloning vector as described below.

Cloning ofH. pylori DNA sequences into the pCR TA cloning vector.

All amplified inserts were cloned into the pCR 2.1 vector by the method described in the Original TA cloning kit (Invitrogen, San Diego, CA). Products of the ligation reaction were then used to transform the TOP 1 OF' (INVaF' in the case of H.

pylori sequence 350) strain of E. coli as described below.

Transformation ofcompetent bacteria with recombinant plasmids Competent bacteria, E coli strain TOP1OF' or E. coli strain INVaF' were transformed with recombinant pCR expression plasmids carrying the cloned H. pylori sequences according to standard methods (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., editors, 1994). Briefly, 2 microliters of 0.5 micromolar BME was added to each vial of 50 microliters of competent cells.

Subsequently, 2 microliters of ligation reaction was mixed with the competent cells and incubated on ice for 30 minutes. The cells and ligation mixture were then subjected to a

"heat shock" at 420C for 30 seconds, and were subsequently placed on ice for an additional 2 minutes, after which, samples were incubated in 0.45 milliliters SOC medium (0.5% yeast extract, 2.0 % tryptone, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl2, 10 mM MgS04 and 20, mM glucose) at 370C with shaking for 1 hour. Samples were then spread on LB agar plates containing 25 microgram/ml kanamycin sulfate or 100 micrograms/ml ampicillan for growth overnight. Transformed colonies of TOP 1 OF' or INVaF' were then picked and analyzed to evaluate cloned inserts as described below.

Identification ofrecombinant PCR plasmids carrying H. pylori sequences Individual TOP1OF' or INVaF' clones transformed with recombinant pCR- H.pylori ORFs were analyzed by PCR amplification of the cloned inserts using the same forward and reverse primers, specific for each H. pylori sequence, that were used in the original PCR amplification cloning reactions. Successful amplification verified the integration of the H. pylori sequences in the cloning vector (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., editors, 1994).

Individual clones of recombinant pCR vectors carrying properly cloned H. pylori ORFs were picked for sequence analysis. Sequence analysis was performed on ABI Sequencers using standard protocols (Perkin Elmer) using vector-specific primers (as found in PCRII or pCR2. 1, Invitrogen, San Diego, CA) and sequencing primers specific to the ORF as listed in Table 8 below.

Table 8 Oligonucleotide primers used for sequencing of H pylori DNA sequences. Outer membrane Forward primers 5' to 3' Reverse Primers 5' to 3' Proteins Protein 26054702 5'- 5'- CCCTTCATTTTAGAAATC CTTTGGGTAAAAACGCA G-3' (SEQ ID NO:179) TC-3' (SEQ ID NO:186) 5'- 5 ATTTCAACCAATTCAAT CGATCTTTGATCCTAATT GCG-3' (SEQ ID NO:180) CA-3' (SEQ ID NO:187) 5 5 GCCCCTTTTGATTTGAAG ATCAAGTTGCCTATGCT CT-3' (SEQ ID NO:181) GA-3' (SEQ ID NO:188) 5 TCGCTCCAAGATACCAA GAAGT-3' (SEQ ID NO:182) 5 CTTGAATTAGGGGCAAA GATCG-3' (SEQ ID NO:183) 5'- ATGCGTTTTTACCCAAA GAAGT-3' (SEQ ID NO:184) 5'- ATAACGCCACTTCCTTAT TGGT-3' (SEQ ID NO:185) Protein 7116626 5 5'- TTGAACACTTTTGATTAT GTCTTTAGCAAAAATGG GCGG-3' (SEQ ID NO:189) CGTC-3' (SEQ ID NO:191) 5 5'- GGATTATGCGATTGTTTT AATGAGCGTAAGAGAGC ACAAG-3' (SEQ ID CTTC-3' (SEQ ID NO:192) NO:190) Protein 5'- 5'- 29479681 CTTATGGGGGTATTGTC AGGTTGTTGCCTAAAGA A-3' (SEQ ID NO:193) CT-3' (SEQ ID NO:195) 5 5'- AGCATGTGGGTATCCAG CTGCCTCCACCTTTGATC C-3' (SEQ ID NO:194) -3' (SEQ ID NO:196)

Protein 346 5'- 5'- ACCAATATCAATTGGCA CTTGCTTGTCATATCTAG CT-3' (SEQ ID NO:197) C-3' (SEQ ID NO:199) 5'- 5'- ACTTGGAAAAGCTCTGC GTTGAAGTGTTGGTGCT A-3' (SEQ ID NO:198) A-3' (SEQ ID NO:200) 5'- 5'- CAAGCAAGTGGTTTGGT GCCCATAATCAAAAAGC TTTAG-3' (SEQ ID NO:201) CCAT-3' (SEQ ID NO:203) 5'- 5'- TGGAAAGAGCAAATCAT CTAAAACCAAACCACTT TGAAG-3' (SEQ ID GCT NO:202) TGTC-3' (SEQ ID NO:204) Vector Primers 5'- 5'- GTAAAACGACGGCCAG- CAGGAAACAGCTATGAC 3' (SEQ ID NO:205) -3' (SEQ ID NO:206) Results To establish the PCR error rate in these experiments, five individual clones of Protein 26054702, prepared from five separate PCR reaction mixtures from H. pylori strain J99, were sequenced over a total length of 897 nucleotides for a cumulative total of 4485 bases of DNA sequence. DNA sequence for the five clones was compared to a DNA sequence obtained previously by a different method, i.e., random shotgun cloning and sequencing. The PCR error rate for the experiments described herein was determined to be 2 base changes out of 4485 bases, which is equivalent to an estimated error rate of less than or equal to 0.04%.

DNA sequence analysis was performed on four different open reading frames identified as genes and amplified by PCR methods from a dozen different strains of the bacterium Helicobacter pylori. The deduced amino acid sequences of three of the four open reading frames that were selected for this study showed statistically significant BLAST homology to defined proteins present in other bacterial species. Those ORFs included: Protein 26054702, homologous to the val A & B genes encoding an ABC transporter in F. novicida; Protein 7116626, homologous to lipoprotein e (P4) present in the outer membrane of H. influenzae; Protein 29479681, homologous to fecA, an outer membrane receptor in iron (III) dicitrate transport in E. coli. Protein 346 was identified as an unknown open reading frame, because it showed low homology with sequences in the public databases.

To assess the extent of conservation or variance in the ORFs across various strains of H pylori, changes in DNA sequence and the deduced protein sequence were compared to the DNA and deduced protein sequences found in the J99 strain of H

pylori (see Table 9 below). Results are presented as percent identity to the J99 strain of H. pylori sequenced by random shotgun cloning. To control for any variations in the J99 sequence each of the four open reading frames were cloned and sequenced again from the J99 bacterial strain and that sequence information was compared to the sequence information that had been collected from inserts cloned by random shotgun sequencing of the J99 strain. The data demonstrate that there is variation in the DNA sequence ranging from as little as 0.12 % difference (Protein 346, J99 strain) to approximately 7% change (Protein 26054702, strain AH5). The deduced protein sequences show either no variation ( Protein 346, strains Ash 18 and AH24) or up to as much as 7.66% amino acid changes (Protein 26054702, Strain AH5).

Table 9 Multiple Strain DNA Sequence analysis of H. pylori Vaccine Candidates J99 Protein II: 26054702 2054702 7116626 7116626 29479681 29479681 346 346 Length of Region Sequenced: 248 a.a. 746 nt. 232 a.a. 96 nt. 182 a.a. 548 nt. 273 a.a. 819 nt.

Strain Tested AA Nuc. AA Nuc. AA Nuc. AA Nuc.

identity identity identity identity identity identity identity identity J99 100.00% 100.00% 100.00% 100.00% 100.00% 100.00% 99.63% 99.88% AH244 95.16% 95.04% n.d. n.d. 99.09% 96.71% 98.90% 96.45% AH4 95.97% 95.98% 97.84% 95.83% n.d. n.d. 97.80% 95.73% AH5 92.34% 93.03% 98.28% 96.12% 98.91% 96.90% 98.53% 95.73% AH15 95.16% 94.91% 97.41% 95.98% 99.82% 97.99% 99.63% 96.09% AH6l n.d. n.d. 97.84% 95.98% 99.27% 97.44% n.d. n.d.

5155 n.d. n.d. n.d. n.d. 99.45°,0 97.08% 98.53% 95.60% 5294 94.35% 94.37% 98.28% 95.40% 99.64% 97.26% 97.07% 95.48% 7958 94.35% 94.10% 97.84% 95.40% n.d. n.d. 99.63% 96.46% 5640 95.16% 94.37% 97.41% 95.69% 99.09% 97.63% 98.53% 95.48% AH18 n.d. n.d. 98.71% 95.69% 99.64% 97.44% 100.00% 95.97% AH24 94.75% 95.04% 97.84% 95.40% 99.27% 96.71% 100.00% 96.46% n.d.= not done.

VI. Experimental Knock-Out Protocol for the Determination of Essential H. pylori Genes as Potential Therapeutic Targets Therapeutic targets are chosen from genes whose protein products appear to play key roles in essential cell pathways such as cell envelope synthesis, DNA synthesis, transcription, translation, regulation and colonization/virulence.

The protocol for the deletion of portions of H. pylori genes/ORFs and the insertional mutagenesis of a kanamycin-resistance cassette in order to identify genes which are essential to the cell is modified from previously published methods (Labigne- Roussel et al., 1988, J. Bacteriology 170, pp. 1704-1708; Cover et al.,1994, J. Biological Chemistry 269, pp. 10566-10573; Reyrat et al., 1995, Proc. Natl. Acad. Sci. 92, pp 8768-8772). The result is a gene "knock-out." Identification and Cloning ofH. pylori Gene Sequences The sequences of the genes or ORFs (open reading frames) selected as knock-out targets are identified from the H. pylori genomic sequence and used to design primers to specifically amplify the genes/ORFs. All synthetic oligonucleotide primers are designed with the aid of the OLIGO program (National Biosciences, Inc., Plymouth, MN 55447, USA), and can be purchased from Gibco/BRL Life Technologies (Gaithersburg, MD, USA). If the ORF is smaller than 800 to 1000 base pairs, flanking primers are chosen outside of the open reading frame.

Genomic DNA prepared from the Helicobacter pylori HpJ99 strain (ATCC 55679; deposited by Genome Therapeutics Corporation, 100 Beaver Street, Waltham, MA 02154) is used as the source of template DNA for amplification of the ORFs by PCR (polymerase chain reaction) (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al.. editors, 1994). For the preparation of genomic DNA from H. pylori, see Example I. PCR amplification is carried out by introducing 10 nanograms of genomic HpJ99 DNA into a reaction vial containing 10 mM Tris pH 8.3, 50 mM KC1, 2 mM MgCl2, 2 microMolar synthetic oligonucleotide primers (forward=F1 and reverse=R1), 0.2 mM of each deoxynucleotide triphosphate (dATP,dGTP, dCTP, dTTP), and 1.25 units of heat stable DNA polymerase (Amplitaq, Roche Molecular Systems, Inc.. Branchburg, NJ, USA) in a final volume of 40 microliters. The PCR is carried out with Perkin Elmer Cetus/GeneAmp PCR System 9600 thermal cyclers.

Upon completion of thermal cycling reactions, each sample of amplified DNA is visualized on a 2% TAE agarose gel stained with Ethidium Bromide (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., editors, 1994) to

determine that a single product of the expected size had resulted from the reaction.

Amplified DNA is then washed and purified using the Qiaquick Spin PCR purification kit (Qiagen, Gaithersburg, MD, USA).

PCR products are cloned into the pT7Blue T-Vector (catalog#69820-1, Novagen, Inc., Madison, WI, USA) using the TA cloning strategy (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., editors, 1994). The ligation of the PCR product into the vector is accomplished by mixing a 6 fold molar excess of the PCR product, 10 ng of pT7Blue-T vector (Novagen), 1 microliter of T4 DNA Ligase Buffer (New England Biolabs, Beverly, MA, USA), and 200 units of T4 DNA Ligase (New England Biolabs) into a final reaction volume of 10 microliters. Ligation is allowed to proceed for 16 hours at 160C.

Ligation products are electroporated (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al.. editors, 1994) into electroporation- competent XL-1 Blue or DH5-a E.coli cells (Clontech Lab., Inc. Palo Alto, CA, USA).

Briefly, 1 microliter of ligation reaction is mixed with 40 microliters of electrocompetent cells and subjected to a high voltage pulse (25 microFarads, 2.5 kV, 200 ohms) after which the samples are incubated in 0.45 ml SOC medium (0.5% yeast extract, 2% tryptone, 10 mM NaCI, 2.5 mM KC1, 10 mM MgCl2, 10 mM MgSO4 and 20 mM glucose) at 3 70C with shaking for 1 hour. Samples are then spread onto LB (10 g/l bacto tryptone, 5 g/l bacto yeast extract, 10 g/l sodium chloride) plates containing 100 microgram/ml of Ampicillin, 0.3% X-gal, and 100 microgram/ml IPTG. These plates are incubated overnight at 370C. Ampicillin-resistant colonies with white color are selected, grown in 5 ml of liquid LB containing 100 microgram/ml of Ampicillin, and plasmid DNA is isolated using the Qiagen miniprep protocol (Qiagen, Gaithersburg, MD, USA).

To verify that the correct H.pylori DNA inserts had been cloned, these pT7Blue plasmid DNAs are used as templates for PCR amplification of the cloned inserts, using the same forward and reverse primers used for the initial amplification of the J99 H.pylori sequence. Recognition of the primers and a PCR product of the correct size as visualized on a 2% TAE, ethidium bromide stained agarose gel are confirmation that the correct inserts had been cloned. Two to six such verified clones are obtained for each knock-out target, and frozen at -700C for storage. To minimize errors due to PCR, plasmid DNA from these verified clones are pooled, and used in subsequent cloning steps.

The sequences of the genes/ORFs are again used to design a second pair of primers which flank the region of H. pylori DNA to be either interrupted or deleted (up to 250 basepairs) within the ORFs but are oriented away from each other. The pool of

circular plasmid DNAs of the previously isolated clones are used as templates for this round of PCR. Since the orientation of amplification of this pair of deletion primers is away from each other, the portion of the ORF between the primers is not included in the resultant PCR product. The PCR product is a linear piece of DNA with H. pylori DNA at each end and the pT7Blue vector backbone between them which, in essence, resultes in the deletion of a portion of the ORFs. The PCR product is visualized on a 1% TAE, ethidium bromide stained agarose gel to confirm that only a single product of the correct size has been amplified.

A Kanamycin-resistance cassette (Labigne-Roussel et al., 1988 J. Bacteriology 170, 1704-1708) is ligated to this PCR product by the TA cloning method used previously (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F.

Ausubel et al., editors, 1994). The Kanamycin cassette containing a Campylobacter kanamycin resistance gene is obtained by carrying out an EcoRI digestion of the recombinant plasmid pCTB8:kan (Cover et al.,1994, J. Biological Chemistry 269, pp.

10566-10573). The proper fragment (1.4 kb) is isolated on a 1% TAE gel, and isolated using the QIAquick gel extraction kit (Qiagen, Gaithersburg, MD, USA). The fragment is end repaired using the Klenow fill-in protocol, which involved mixing 4ug of the DNA fragment, 1 microliter of dATP,dGTP, dCTP, dTTP at 0.5 mM, 2 microliter of Klenow Buffer (New England Biolabs) and 5 units of Klenow DNA Polymerase I Large (Klenow) Fragment (New England Biolabs) into a 20 microliter reaction, incubating at 300C for 15 min, and inactivating the enzyme by heating to 750C for 10 minutes. This blunt-ended Kanamycin cassette is then purified through a Qiaquick column (Qiagen, Gaithersburg, MD, USA) to eliminate nucleotides. The "T" overhang is then generated by mixing 5 micrograms of the blunt-ended kanamycin cassette, 10 mM Tris pH 8.3, 50 mM KCI, 2 mM MgCl2, 5 units of DNA Polymerase (Amplitaq, Roche Molecular Systems, Inc., Branchburg, NJ, USA), 20 microliters of 5 mM dTTP, in a 100 microliter reaction and incubating the reaction for 2 hours at 370C. The "Kan-T" cassette is purified using a QIAquick column (Qiagen, Gaithersburg, MD, USA). The PCR product of the deletion primers (F2 and R2) is ligated to the Kan-T cassette by mixing 10 to 25 ng of deletion primer PCR product, 50 - 75 ng Kan-T cassette DNA, 1 microliter 10x T4 DNA Ligase reaction mixture, 0.5 microliter T4 DNA Ligase (New England Biolabs, Beverly, MA, USA) in a 10 microliter reaction and incubating for 16 hours at 160C.

The ligation products are transformed into XL-1 Blue or DH5-a E.coli cells by electroporation as described previously. After recovery in SOC, cells are plated onto LB plates containing 100 microgram/ml Ampicillin and grown overnight at 370C. These plates are then replica plated onto plates containing 25 microgram/ml Kanamycin and

allowed to grow overnight. Resultant colonies have both the Ampicillin resistance gene present in the pT7Blue vector, and the newly introduced Kanamycin resistance gene.

Colonies are picked into LB containing 25 microgram/ml Kanamycin and plasmid DNA is isolated from the cultured cells using the Qiagen miniprep protocol (Qiagen, Gaithersburg, MD. USA).

Several tests by PCR amplification are conducted on these plasmids to verify that the Kanamycin is inserted in the H. pylori gene/ORF, and to determine the orientation of the insertion of the Kanamycin-resistance gene relative to the H. pylori gene/ORF. To verify that the Kanamycin cassette is inserted into the H. pylori sequence, the plasmid DNAs are used as templates for PCR amplification with the set of primers originally used to clone the H. pylori gene/ORFs. The correct PCR product is the size of the deleted gene/ORF but increased in size by the addition of a 1.4 kilobase Kanamycin cassette. To avoid potential polar effects of the kanamycin resistance cassette on H.

pylori gene expression, the orientation of the Kanamycin resistance gene with respect to the knock-out gene/ORF is determined and both orientations are eventually used in H.

pylori transformations (see below). To determine the orientation of insertion of the kanamycin resistance gene, primers are designed from the ends of the kanamycin resistance gene ("Kan-l" 5'-ATCTTACCTATCACCTCAAAT-3' (SEQ ID NO:207)), and "Kan-2" 5'-AGACAGCAACATCTTTGTGAA-3' (SEQ ID NO:208)). By using each of the cloning primers in conjunction with each of the Kan primers (4 combinations of primers), the orientation of the Kanamycin cassette relative to the H.pylori sequence is determined. Positive clones are classified as either in the "A" orientation (the same direction of transcription is present for both the H. pylori gene and the Kanamycin resistance gene), or in the "B" orientation (the direction of transcription for the H. pylori gene is opposite to that of the Kanamycin resistance gene). Clones which share the same orientation (A or B) are pooled for subsequent experiments and independently transformed into H. pylon.

Transformation of Plasmid DNA into H. pylori cells Two strains of H. pylori are used for transformation: ATCC 55679, the clinical isolate which provided the DNA from which the H. pylori sequence database is obtained, and AH244, an isolate which had been passaged in, and has the ability to colonize the mouse stomach. Cells for transformation are grown at 370C, 10% CO2, 100% humidity, either on Sheep-Blood agar plates or in Brucella Broth liquid. Cells are grown to exponential phase, and examined microscopically to determine that the cells are "healthy" (actively moving cells) and not contaminated. If grown on plates, cells are harvested by scraping cells from the plate with a sterile loop, suspended in 1 ml of

Brucella Broth, spun down (1 minute, top speed in eppendorf microfuge) and resuspended in 200 microliters Brucella Broth. If grown in Brucella Broth liquid, cells are centrifuged (15 minutes at 3000 rpm in a Beckman TJ6 centrifuge) and the cell pellet resuspended in 200 microliters of Brucella broth. An aliquot of cells is taken to determine the optical density at 600 nm, in order to calculate the concentration of cells.

An aliquot (1 to 5 OD600 units/25 microliter) of the resuspended cells is placed onto a prewarmed Sheep-Blood agar plate, and the plate is further incubated at 370C, 6% CO2, 100% humidity for 4 hours. After this incubation, 10 microliters of plasmid DNA (100 micrograms per microliter) is spotted onto these cells. A positive control (plasmid DNA with the ribonuclease H gene disrupted by kanamycin resistance gene) and a negative control (no plasmid DNA) are done in parallel. The plates are returned to 370C, 6% CO2 for an additional 4 hours of incubation. Cells are then spread onto that plate using a swab wetted in Brucella broth, and grown for 20 hours at 370C, 6% CO2. Cells are then transferred to a Sheep-Blood agar plate containing 25 micrograms/ml Kanamycin, and allowed to grow for 3 to 5 days at 370C, 6% CO2, 100% humidity. If colonies appear, they are picked and regrown as patches on a fresh Sheep-Blood agar plate containing 25 micrograms/ml Kanamycin.

Three sets of PCR tests are done to verify that the colonies of transformants have arisen from homologous recombination at the proper chromosomal location. The template for PCR (DNA from the colony) is obtained by a rapid boiling DNA preparation method as follows. An aliquot of the colony (stab of the colony with a toothpick) is introduced into 100 microliters of 1% Triton X-100, 20 mM Tris, pH 8.5, and boiled for 6 minutes. An equal volume of phenol : chloroform (1:1) is added and vortexed. The mixture is microfuged for 5 minutes and the supernatant is used as DNA template for PCR with combinations of the following primers to verify homologous recombination at the proper chromosomal location.

TEST 1. PCR with cloning primers originally used to amplify the gene/ORF. A positive result of homologous recombination at the correct chromosomal location should show a single PCR product whose size is expected to be the size of the deleted gene/ORF but increased in size by the addition of a 1.4 kilobase Kanamycin cassette. A PCR product ofjust the size of the gene/ORF is proof that the gene had not been knocked out and that the transformant is not the result of homologous recombination at the correct chromosome location.

TEST 2. PCR with F3 (primer designed from sequences upstream of the gene/ORF and not present on the plasmid), and either primer Kan- 1 or Kan-2 (primers designed from the ends of the kanamycin resistance gene), depending on whether the plasmid DNA used was of "A" or "B" orientation. Homologous recombination at the

correct chromosomal location will result in a single PCR product of the expected size (i.e., from the location of F3 to the insertion site of kanamycin resistance gene). No PCR product or PCR product(s) of incorrect size(s) will prove that the plasmid had not integrated at the correct site and that the gene had not been knocked out.

TEST 3. PCR with R3 (primer designed from sequences downstream of the gene/ORF and not present on the plasmid) and either primer Kan-l or Kan-2, depending on whether the plasmid DNA used was of "A" or "B" orientation. Homologous recombination at the correct chromosomal location will result in a single PCR product of the expected size (i.e., from the insertion site of kanamycin resistance gene to the downstream location of R3). Again, no PCR product or PCR product(s) of incorrect size(s) will prove that the plasmid had not integrated at the correct site and that the gene had not been knocked out.

Transformants showing positive results for all three tests above indicate that the gene is not essential for survival in vitro.

A negative result in any of the three above tests for each transformant indicates that the gene had not been disrupted, and that the gene is essential for survival in vitro.

In the event that no colonies result from two independent transformations while the positive control with the disrupted ribonuclease H plasmid DNA produces transformants, the plasmid DNA is further analyzed by PCR on DNA from transformant populations prior to plating for colony formation. This will verify that the plasmid can enter the cells and undergo homologous recombination at the correct site. Briefly, plasmid DNA is incubated according to the transformation protocol described above.

DNA is extracted from the H. pylori cells immediately after incubation with the plasmid DNAs and the DNA is used as template for the above TEST 2 and TEST 3. Positive results in TEST 2 and TEST 3 would verify that the plasmid DNA could enter the cells and undergo homologous recombination at the correct chromosomal location. If TEST 2 and TEST 3 are positive, then failure to obtain viable transformants indicates that the gene is essential, and cells suffering a disruption in that gene are incapable of colony formation VII. Hih-throuhput drug screen assay Cloning, expression and protein pllrification Cloning, transformation, expression and purification of the H. pylori target gene and its protein product,e.g., an H. pylori enzyme, to be used in a high-throughput drug screen assay, is carried out essentially as described in Examples II and III above.

Development and application of a screening assay for a particular H. pylori gene product, peptidyl-propyl cis-trans isomerase. is described below as a specific example.

Enzymatic Assay The assay is essentially as described by Fisher (Fischer, G., et.al. (1984) Biomed.

Biochim. Acta 43:1101-1111). The assay measures the cis-trans isomerization of the Ala-Pro bond in the test peptide N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide (Sigma It S- 7388, lot It 84H5805). The assay is coupled with a-chymotrypsin, where the ability of the protease to cleave the test peptide occurs only when the Ala-Pro bond is in trans.

The conversion of the test peptide to the trans isomer in the assay is followed at 390 nm on a Beckman Model DU-650 spectophotometer. The data are collected every second with an average scanning of time of 0.5 second. Assays are carried out in 35 mM Hepes, pH 8.0, in a final volume of 400 ul, with 10 pM ot-chymotrypsin (type 1-5 from bovine Pancreas, Sigma It C-7762, lot 23H7020) and 10 nM PPIase. To initiate the reaction, 10 ,ul of the substrate ( 2 mM N-Succinyl-Ala-Ala-Pro-Phe-p-nitroanilide in DMSO) is added to 390 pl of reaction mixture at room temperature.

Enzymatic assay in crude bacterial extract.

A 50 ml culture of Helicobacter pylori (strain J99) in Brucella broth is harvested at mid-log phase (OD 600 nm c. 1) and resuspended in lysis buffer with the following protease inhibitors: 1 mM PMSF, and 10 ,ug/ml of each of aprotinin, leupeptin, pepstatine, TLCK, TPCK, and soybean trypsin inhibitor. The suspension is subjected to 3 cycles of freeze-thaw (15 minutes at -70 C, then 30 minutes at room temperature), followed by sonication (three 20 second bursts). The lysate is centrifuged (12,000 g x 30 minutes) and the supernatant is assayed for enzymatic activity as described above.

Many H. pylori enzymes can be expressed at high levels and in an active form in E. coli. Such high yields of purified proteins provide for the design of various high throughput drug screening assays.

EOUIVALENTS Those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments and methods described herein. Such equivalents are intended to be encompassed by the scope of the following claims.

SEQUENCE LISTING 1) GENERAL INFORMATION: (i) APPLICANT: (A) NAME: Astra Aktiebolag (B) STREET: S-151 85 (C) CITY: Sodertalje (D) STATE: (E) COUNTRY: Sweden (F) POSTAL CODE (ZIP) (ii) TITLE OF INVENTION: NUCLEIC ACID AND AMINO ACID SEQUENCES RELATING TO HELICOBACTER PYLORI AND VACCINE COMPOSITIONS THEREOF (iii) NUMBER OF SEQUENCES: 208 (iv) COMPUTER READABLE FORM: (A) MEDIUM TYPE: (B) COMPUTER: (C) OPERATING SYSTEM: (D) SOFTWARE: (v) CURRENT APPLICATION DATA: (A) APPLICATION NUMBER (B) FILING DATE: (vi) PRIOR APPLICATION DATA: (A) APPLICATION NUMBER:US 08/739,150 (B) FILING DATE: 28-OCT-1996 (vii) PRIOR APPLICATION DATA: (A) APPLICATION NUMBER: US 08/759,739 (B) FILING DATE: 06-DEC-1996 (viii) PRIOR APPLICATION DATA: (A) APPLICATION NUMBER: US 08/891,928 (B) FILING DATE: 14-JULY-1997 (ix) CORRESPONDENCE ADDRESS: (A) ADDRESSEE: LAHIVE & COCKFIELD (B) STREET: 28 State Street (C) CITY: Boston (D) STATE: Massachusetts (E) COUNTRY: USA (F) ZIP: 02109-1875 (x) ATTORNEY/AGENT INFORMATION: (A) NAME: Mandragouras, Amy E.

(B) REGISTRATION NUMBER: 36,207 (C) REFERENCE/DOCKET NUMBER: GTN-001CP10PC

(xi) TELECOMMUNICATION INFORMATION: (A) TELEPHONE: (617)227-7400 (B) TELEFAX: (617)742-4214 (2) INFORMATION FOR SEQ ID NO:1: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 561 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...561 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:1: ATGATTAAAA GAATTGCTTG TATTTTAAGC TTGAGCGCGA GTTTAGCGTT AGCTGGCGAA 60 GTGAATGGGT TTTTCATGGG TGCGGGTTAT CAACAAGGTC GTTATGGCCC TTATAACAGC 120 AATTACTCTG ATTGGCGTCA TGGCAATGAC CTTTATGGTT TGAATTTCAA ATTAGGTTTT 180 GTAGGCTTTG CCAATAAATG GTTTGGGGCT AGGGTGTATG GCTTTTTAGA TTGGTTTAAC 240 ACTTCAGGGA CTGAACACAC CAAAACCAAT TTGCTCACCT ATGGCGGCGG TGGCGATTTG 300 ATTGTCAATC TCATTCCTTT GGATAAATTC GCTCTAGGTC TCATTGGTGG CGTTCAATTA 360 GCCGGAAACA CTTGGATGTT CCCTTATGAT GTCAATCAAA CCAGATTCCA GTTCTTATGG 420 AATTTAGGCG GAAGAATGCG TGTTGGGGAT CGCAGTGCGT TTGAAGCGGG CGTGAAATTC 480 CCTATGGTTA ATCAGGGTAG CAAAGATGTA GGGCTTATCC GCTACTATTC TTGGTATGTG 540 GATTATGTCT TCACTTTCTA G 561 (2) INFORMATION FOR SEQ ID NO:2: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 351 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori

(ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...351 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2: TTGATGCGCA TTATCATAAG GTTACTTTCA TTTAAAATGA ACGCTTTTTT AAAACTCGCG 60 CTCGCTTCTT TGATGGGGGG GCTTTGGTAT GCTTTCAATG GCGAAGGCTC TGAGATTGTC 120 GCTATAGGGA TTTTTGTGTT GATCTTGTTT GTTTTTTTTA TCCGCCCTGT GAGTTTCCAA 180 GACCCAGAAA AACGAGAAGA ATACATAGAA CGGCTTAAAA AAAACCATGA GAGGAAAATG 240 ATCTTACAAG ACAAGCAAAA AGAAGAGCAA ATGCGCCTCT ATCAAGCCAA AAAAGAGCGA 300 GAGAGCAGGC AAAAACAAGA CCTTAAAGAA CAAATGAAAA AATACTCATA A 351 (2) INFORMATION FOR SEQ ID NO:3: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1038 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...1038 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3: ATGGTTAAAC ACTATCTTTT CATGGCGGTT TCGCAGGTCT TTTTCTCCTT CTTTTTAGTG 60 CTGTTTTTTA TCTCTTCCAT TGTGTTATTA ATCAGTATTG CAAGCGTAAC GCTCGTGATT 120 AAAGTGAGCT TTTTGGATCT GGTGCAACTC TTTTTGTATT CCTTGCCAGG AACCATTTTT 180 TTTATTTTGC CGATCACTTT TTTTGCGGCT TGCGCTTTGG GGCTTTCAAG GCTTAGCTAT 240 GACCATGAAT TGTTAGTGTT TTTCTCTTTA GGGGTTTCGC CTAAAAAAAT GACTAAAGCG 300 TTTGTGCCTT TAAGTTTGTT AGTGAGCGCG ATTTTATTAG CGTTTTCGCT CATCTTAATC 360 CCCACTTCTA AGAGCGCTTA TTACGGGTTT TTGCGTCAAA AAAAAGACAA GATTGACATT 420 AACATCAGAG CGGGTGAATT CGGGCAAAAA TTAGGCGATT GGCTCGTGTA TGTGGATAAG 480 ACTGAAAACA ATTCCTATGA TAATTTGGTG CTTTTTTCTA ATAAAAGTCT CTCTCAAGAA 540 AGCTTTATTT TGGCTCAAAA AGGCAATATC AACAATCAAA ACGGCGTGTT TGAATTGAAT 600 TTGTATAACG GGCATGCGTA TTTCACTCAA GGCGATAAAA TGCGTAAGGT TGATTTTGAA 660 GAATTGCATT TGCGCAACAA GCTCAAGTCT TTCAATTCTA ATGATGCGGC TTATTTGCAA 720 GGCACGGATT ATTTGGGTTA TTGGAAAAAA GCCTTTGGTA AAAACGCTAA TAAAAATCAA 780 AAACGCCGTT TTTCTCAAGC GATCTTAGTT TCCTTGTTCC CTTTAGCGAG CGTGTTTTTA 840 ATCCCCTTAT TTGGCATCGC CAACCCGCGA TTCAAAACGA ATTGGAGTTA TTTCTATGTC 900 CTTGGAGCGG TTGGGGTTTA TTTTTTAATG GTGCATGTGA TTTCTACGGA TTTGTTTTTG 960 ATGACCTTTT TCTTCCCCTT TATTTGGGCG TTTATTTCTT ATTTATTGTT TAGAAAATTC 1020 ATTTTAAAGC GTTATTAA 1038

(2) INFORMATION FOR SEQ ID NO:4: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 831 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...831 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4: ATGAAGAAAA AAGCAAAAGT CTTTTGGTGT TGTTTTAAAA TGATTCGTTG GTTGTATTTG 60 GCGGTCTTTT TTTTGTTGAG CGTATCAGAC GCTAAAGAAA TCGCTATGCA ACGATTTGAC 120 AAACAAAACC ATAAGATTTT TGAAATCCTT GCGGATAAAG TGAGCGCCAA AGACAATGTG 180 ATAACCGCCT CAGGGAATGC GATCCTATTG AATTATGACG TGTATATTCT AGCGGATAAG 240 GTGCGTTATG ACACCAAGAC TAAAGAAGCG TTATTAGAAG GCAATATTAA GGTTTATAGG 300 GGCGAGGGCT TGCTCGTTAA AACCGATTAT GTGAAATTGA GTTTGAACGA AAAATATGAG 360 ATCATTTTCC CCTTTTATGT CCAAGACAGC GTGAGCGGGA TTTGGGTGAG CGCGGATATT 420 GCTAGCGGGA AGGATCAAAA ATATAAGATT AAAAACATGA GCGCTTCAGG GTGCAGCATT 480 GACAACCCCA TTTGGCATGT CAATGCGACT TCAGGCTCAT TTAACATGCA AAAATCGCAT 540 TTGTCAATGT GGAATCCTAA GATTTATGTC GGCGATATTC CTGTATTGTA TTTGCCCTAT 600 ATTTTCATGT CCACGAGCAA TAAAAGAACT ACCGGGTTTT TATACCCTGA GTTTGGCACT 660 TCCAACTTAG ACGGCTTTAT TTATTTGCAA CCCTTTTATT TAGCCCCCAA AAACTCATGG 720 GATATGACCT TTACCCCACA AATCCGTTAC AAAAGGGGTT TTGGCTTGAA TTTTGAAGCG 780 CGCTACATCA ACTCTAAGAC GCAGGTTTTT ATTCAATGCG CGCTATTTTA G 831 (2) INFORMATION FOR SEQ ID NO:5: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 675 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori

(ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...675 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5: ATGATTAGAT TAAAAGGTTT GAATAAACT TTAAAAACAA GCTTATTAGC TGGGGTTTTA 60 CTAGGTGCTA CTGCTCCCTT AATGGCAAAG CCTTTATTAA GCGATGAAGA CTTATTGAAA 120 CGAGTAAAAC TACACAATAT CAAAGAAGAT ACGCTGACTA GCTGTAATGC TAAGGTGGAC 180 GGCTCTCAAT ACTTGAATAG TGGTTGGAAT TTATCTAAAG AATTTCCGCA AGAATATAGA 240 GAAAAGATTT TTGAATGCGT AGAAGAAGAA AAACATAAAC AAGCCCTTAA TTTAATCAAT 300 AAAGAAGACA CTAAAGATAA AGAAGAACTT GCAWAA TCAAAGAAAT TAAAGAAAAA 360 GCTAAAGTTT TAAGGCAAAA ATTTATGGCT TTTGAAATGA AAGAACACTC TAAAGAATTC 420 CCAAATAAAA AGCAACTTCA AACCATGCTT GAGAACGCTT TTGATAATGG AGCTGAAAGT 480 TTTATTGATG ATTGGCACGA ACGCTTTGGG GGTATAAGTA GAGAGAATAC TTATAAAGCA 540 CTTGGCATTA AAGAATATAG TGATGAAGGA AAGATATTGC CTTTGGCGAA AGAAGTTATA 600 TTAGACAATA TAAAAAAGAT TTTGAAGAAA GCACTTATGA TACTAGACAA CCCTTATCTG 660 CTATGGCTAG TATGA 675 (2) INFORMATION FOR SEQ ID NO:6: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1290 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...1290 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:6: ATGCCATACG CCTTAAGAAA AAGATTTTTC AAACGCCTTT TATTGTTTTT TTTAATTGTT 60 TGTATGATAA ATTTGCATGC CAAAAGCTAT CTGTTTTCTC CTTTGCCCCC AGCGCACCAG 120 CAAATCATTA AGACAGAGCC TTGCTCTTTG GAGTGCTTGA AAGACTTGAT GCTGCAAAAT 180 CAAATCTTTT CTTTTGTATC CCAATACGAT GATAACAACC AAGATGAGAG CCTTAAAACT 240 TATTACAAGG ACATCTTAAA CAAACTCAAC CCCGTATTCA TCGCTTCTCA AACTCCAGCT 300 AAAGAAAGCT ATGAGCCTAA GATTGAATTA GCGATTTTAC TGCCTAAAAA GGTGGTGGGC 360 CGTTATGCGA TTTTAGTGAT GAACACCCTT TTAGCGTATT TGAACACCAG AAACAACGAT 420 TTCAATATCC AAGTCTTTGA CAGCGATGAA GAAAGCCCTG AAAAATTAGA AGAAACCTAT 480 AAAGAAATTG AAAAAGAAAA ATTCCCTTTT ATCATCGCTT TATTGACTAA AGAGGGCGTG 540 GAAAATTTGC TCCAAAATAC GACTATCAAT ACCCCTACTT ATGTGCCTAC GGTGAATAAA 600 ACGCAATTAG AAAATCATAC CGAGCTTTCT TTAAGCGAGC GCTTGTATTT TGGGGGGATT 660 GATTATAAAG AGCAATTAGG CATGCTCGCA ACTTTCATTA GCCCTAATTC GCCCGTGATT 720 GAATACGATG ATGATGGCCT GATAGGTGAA CGCTTGAGGC AAATCACGGA GTCTTTAAAC 780

GTTGAAGTCA AACACCAAGA AAACATTTCT TACAAACAAG CGACCAGTTT TTCTAAAAAT 840 TTTAGAAAAC ATGATGCGTT TTTTAAAAAT TCTACCTTAA TTTTGAACAC CCCTACCACT 900 AAAAGCGGTC TGATCCTTTC TCAAATAGGG CTTTTAGAGT ATAAGCCTCT TAAAATCCTT 960 TCCACACAAA TCAATTTCAA CCCCTCTTTA CTCTTGCTCA CCCAGCCTAA AGACAGGAAA 1020 AATTTATTCA TTGTCAATGC CTTGCAAAAC AGCGATGAAA CGCTGATAGA ATACGCTTCC 1080 TTATTAGAGA GCGATTTAAG GCATGATTGG GTGAATTATT CCAGCGCGAT AGGGCTAGAG 1140 ATGTTTTTAA ACACGCTAGA TCCGCATTTT AAAAAGTCTT TTCAAGAGAG TTTGGAAGAC 1200 AATCAAGTCC GTTACCACAA TCAAATTTAT CAGGCTTTAG GGTATTCTTT TGAGCCGATA 1260 AAAAACGAAA GCGAAACAAA AAAAGAATAA 1290 (2) INFORMATION FOR SEQ ID NO:7: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1368 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...1368 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:7: GTGTTAAAAT TTCAAAAATT ACCCTTATTG TTTGTTTCCA TTCTTTATAA TCAAAGCCCT 60 TTATTGGCTT TTGATTATAA GTTTAGTGGG GTAGCGGAAT CTGTTTCTAA AGTGGGGTTT 120 AACCATTCCA AACTCAATTC CAAAGAAGGG ATTTTCCCTA CAGCCACCTT TGTAACCGCC 180 ACGATCAAGC TTCAAGTGGA TTCCAATCTG CTCCCTAAAA ACATTGAAAA ACACAGCTTA 240 AAAATAGGCG TTGGGGGGAT TTTAGGAGCG CTCGCTTACG ATTCCACCAA AACGCTCATA 300 GACCAAGCCA CGCATCAAAT CTATGGCTCA GAACTTTTTT ACCTCATAGG GCGTTGGTGG 360 GGGTTTTTAG GCAACGCTCC TTGGAAAGAC TCCCTCATAG AATCTGACGC TCACACCCGT 420 AATTATGTGC TGTATAATTC CTATCTGTTT TATTCTTATG GCGATAAATT CCACCTAAAA 480 TTAGGGCGTT ATCTCTCTAA CATGGATTTT ATGAGTTCCT ACACACAGGG TTTTGAACTG 540 GATTATAAAA TCAATTCTAA AATAGCGTTA AAATGGTTTA GCTCTTTTGG GAGGGCGTTG 600 GCTTTTGGGC AATGGATACG GGATTGGTAT GCCCCTATTG TAACTGAAGA TGGCAGAAAA 660 GAAGTTTATG ATGGCATCCA TGCCGCGCAA CTCTATTTTT CTAGCAAGCA TGTTCAAGTC 720 ATGCCTTTTG CTTATTTTTC GCCTAAGATT TACGGAGCGC CCGGTGTTAA AATCCATATT 780 GATAGCAACC CGAAATTCAA AGGCTTAGGG TTAAGGGCTC AAACCACTAT TAATGTGATT 840 TTCCCTGTTT ATGCTAAAGA TTTATACGAT GTGTATTGGC GTAACTCTAA GATTGGCGAG 900 TGGGGCGCAT CGCTTTTGAT CCACCAACGC TTTGACTACA ACGAATTTAA CTTTGGCTTT 960 GGTTATTACC AAAATTTTGG CAACGCTAAC GCAAGGATTG GCTGGTATGG TAACCCCATC 1020 CCTTTTAATT ATAGAAATAA CAGCGTTTAT GGTGGGGTCT TCAGTAACGC TATTACCGCA 1080 GACGCCGTTT CTGGGTATGT CTTTGGTGGG GGGGTGTATA GAGGGTTTTT ATGGGGTATT 1140 TTAGGCAGAT ACACTTATGC CACTAGAGCG AGCGAAAGAT CCATCAACTT GAACTTGGGC 1200 TATAAATGGG GTTCTTTTGC TAGAGTTGAT GTGAATTTAG AATACTATGT GGTCAGCATG 1260 CACAACGGCT ATAGATTAGA CTATCTCACC GGCCCTTTCA ACAAAGCCTT TAAGGCTGAC 1320

GCACAAGATA GGAGTAACCT TATGGTTAGC ATGAAATTCT TTTTTTAA 1368 (2) INFORMATION FOR SEQ ID NO:8: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 849 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...849 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:8: ATGGGGTGTT CGTTTATCTT TAAAAAAGTT AGGGTTTATT CTAAAATGTT GGTTGCTTTG 60 GGGCTTTCAA GCGTGTTGAT CGGTTGCGCG ATGAATCCAA GCGCTGAGAC AAAAAAACCA 120 AATGACGCCA AAAACCAACA ACCAGTTCAA ACTCATGAAA GAATGACAAC AAGTTCTGAA 180 CATGTTACGC CACTAGATTT TAATTACCCG GTGCATATTG TTCAAGCCCC ACAAAACCAT 240 CATGTTGTAG GTATTTTAAT GCCACGCATT CAAGTGAGCG ATAATCTAAA ACCCTATATT 300 GATAAGTTTC AAGACGCTTT AATTAATCAA ATCCAAACTA TTTTTGAAAA AAGAGGCTAT 360 CAAGTGTTGC GTTTTCAAGA TGAAAAAGCT TTGAATGTGC AAGATAAGAA AAAGATTTTT 420 TCCGTTTTGG ATTTGAAAGG GTGGGTAGGA ATCTTAGAAG ATTTGAAAAT GAATTTAAAA 480 GATCCCAATA GTCCCAATTT AGACACGCTA GTGGATCAAA GCTCAGGCTC TGTATGGTTT 540 AATTTTTATG AACCAGAAAG CAATCGTGTC GTCCATGATT TTGCTGTAGA AGTAGGAACT 600 TTTCAGGCAA TAACATACAC ATACACCTCT ACTAATAACG CTTCAGGAGG GTTTAATTCT 660 TCAAAAAGCG TTATCCATGA AAATTTGGAT AAGAATAGAG AAGACGCGAT ACACAAGATT 720 TTAAACAGAA TGTATGCGGT TGTCATGAAA AAAGCTGTAA CAGAACTTAC AAAAGAAAAT 780 ATCGCCAAAT ACAGAGACGC TATTGATAGA ATGAAAGGCT TTAAAAGTTC TATGCCTCAA 840 AAAAAGTAG 849 (2) INFORMATION FOR SEQ ID NO:9: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 843 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO

(vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...843 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:9: ATGAAACTGA GAGCAAGTGT TTTAATCGGT GTGGCAATTC TGTGCTTAAT TTTAAGTGCG 60 TGCAGTAACT ATGCGAAAAA AGTGGTGAAA CAAAAGAACC ATGTTTATAC GCCTGTGTAT 120 AATGAACTGA TAGAGAAGTA TAGTGAGATC CCCTTAAATG ACAAACTCAA AGACACACCA 180 TTCATGGTGC AAGTGAAGTT GCCAAATTAC AAGGACTATT TGTTGGATAA TAAACAAGTT 240 GTACTAACTT TCAAACTTGT TCACCATTCT AAAAAGATTA CGCTCATAGG CGATGCCAAT 300 AAGATCCTCC AATACAAGAA TTACTTCCAA GCTAACGGGG CAAGATCTGA CATTGATTTT 360 TACTTGCAAC CCACTTTGAA TCAAAAGGGT GTGGTGATGA TAGCGAGTAA CTACAATGAT 420 AATCCCAACA ACAAAGAAAA ACCACAGACC TTTGATGTGT TGCAAGGAAG TCAGCCAATG 480 CTAGGAGCTA ACACAAAAAA CTTGCATGGC TATGATGTGA GTGGAGCAAA CAACAAGCAA 540 GTGATCAATG AAGTGGCAAG AGAAAAAGCT CAGCTAGAAA AAATCAATCA GTATTACAAG 600 ACTCTCTTGC AAGACAAGGA ACAAGAATAT ACCACTAGGA iZFLAATAACCA ACGAGAAATT 660 TTAGAAACAT TGAGTAATCG TGCAGGTTAT CAAATGAGGC AGAATGTGAT TAGTTCTGAG 720 ATTTTTAAGA ATGGCAACTT GAACATGCAA GCCAAAGAAG AAGAAGTTAG GGAGAAGCTA 780 CAAGAAGAAA GAGAGAATGA ATACTTGCGC AATCAAATCA GAAGTTTGCT CAGTGGTAAG 840 TGA 843 (2) INFORMATION FOR SEQ ID NO:10: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1179 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...1179 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:10: ATGAGAAAAC TATTCATCCC ACTTTTATTA TTCAGCGCTT TAGAAGCGAA CGAGAAAAAC 60 GGCTTTTTCA TAGAAGCCGG CTTTGAAACT GGGCTATTAG AAGGCACACA AACGCAAGAA 120 AAAAGACACA CCACCACAAA AAACACTTAC GCAACTTACA ATTATTTACC CACAGACACG 180 ATTTTAAAAA GAGCGGCTAA TTTATTCACC AATGCCGAAG CGATTTCAAA ATTAAAATTC 240 TCATCTTTAT CCCCTGTTAG AGTGTTGTAT ATGTATAATG GTCAATTAAC TATAGAAAAC 300 TTCTTGCCTT ATAATTTAAA TAATGTTAAG CTTAGTTTTA CAGACGCTCA AGGCAATGTG 360 ATCGATCTAG GCGTGATAGA GACTATCCCC AAACACTCTA AGATTGTTTT GCCCGGAGAG 420

GCATTTGATA GTCTAAAAAT TGACCCCTAT ACTTTATTTC TTCCAAAAAT TGAAGCCACT 480 AGCACTTCTA TTTCTGACGC TAACACGCAG AGGGTGTTTG AAACGCTCAA TAAGATTAAG 540 ACAAATTTGG TCGTAAATTA TAGGAATGAA AACAAATTTA AAGATCACGA AAATCATTGG 600 GAAGCCTTTA CCCCACAAAC CGCAGAAGAA TTCACTAATT TAATGTTGAA CATGATCGCT 660 GTTTTAGACT CCCAATCTTG GGGCGATGCG ATCTTAAACG CTCCTTTTGA GTTCACTAAC 720 AGCCCAACAG ATTGCGATAA TGATCCTTCA AAATGCGTAA ATCCTGGGAC AAACGGGCTT 780 GTCAATTCTA AAGTCGATCA AAAATATGTG TTAAACAAAC AAGACATTGT CAATAAATTT 840 AAAAACAAAG CGGATCTTGA TGTAATTGTT TTAAAGGATT CAGGGGTTGT AGGGCTTGGG 900 AGTGATATTA CCCCTAGCAA CAATGATGAT GGCAAGCATT ATGGCCAGTT AGGGGTAGTA 960 GCTTCTGCTT TAGATCCTAA AAAACTCTTT GGCGATAACC TTAAGACTAT CAATTTAGAG 1020 GATTTAAGAA CCATCTTGCA TGAATTCAGC CACACTAAAG GCTATGGGCA TAACGGGAAT 1080 ATGACCTATC AAAGAGTGCC GGTAACGAAA GATGGTCAAG TGGAAAAGGA TAGTAATGGC 1140 AAGCCAAAAG ATTCTGATGG CCTCCCCTAT AATGTGTGT 1179 (2) INFORMATION FOR SEQ ID NO:11: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 813 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...813 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:l1: ATGAAAAAGT TTGTAGCTTT AGGGCTTCTA TCCGCGGTTT TAAGCTCTTC GTTGTTAGCC 60 GAAGGTGATG GTGTTTATAT AGGGACTAAT TATCAGCTTG GACAAGCCCG TTTGAATAGC 120 AATATTTATA ATACAGGGGA TTGCACAGGG AGTGTTGTAG GTTGCCCCCC AGGTCTTACC 180 GCTAATAAGC ATAATCCAGG AGGCACCAAT ATCAATTGGC ACTCCAAATA CGCTAATGGG 240 GCTTTGAATG GTTTTGGGTT GAATGTGGGT TATAAGAAAT TCTTCCAATT CAAGTCGCTA 300 GATATGACAA GCAAGTGGTT TGGTTTTAGA GTGTATGGGC TTTTTGATTA CGGGCATGCC 360 GATTTAGGTA AACAAGTTTA TGCACCTAAT AAAATCCAGT TGGATATGGT CTCTTGGGGT 420 GTGGGGAGCG ATTTGTTAGC TGATATTATT GATAAAGACA ACGCTTCTTT TGGTATTTTT 480 GGTGGGGTCG CTATCGGCGG TAACACTTGG AAAAGCTCTG CAGCAAACTA TTGGAAAGAG 540 CAAATCATTG AAGCCAAAGG TCCTGATGTT TGTACCCCTA CTTATTGTAA CCCTAATGCC 600 CCTTATAGCA CCAACACTTC AACCGTCGCT TTTCAAGTGT GGTTGAATTT TGGGGTGAGA 660 GCCAATATCT ACAAGCATAA TGGCGTGGAA TTTGGCGTGA GAGTGCCGCT ACTCATCAAT 720 AAATTTTTGA GCGCGGGTCC TAACGCTACT AACCTTTATT ACCATTTGAA ACGGGATTAT 780 TCGCTTTATT TGGGGTATAA CTACACTTTT TAA 813 (2) INFORMATION FOR SEQ ID NO:12: (i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 423 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...423 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:12: ATGCATCCTA TAATGTTTGC CTATATCGCT AACGCGCTCG CTCAAGCTAG AAAGATCAAC 60 GGAACACTTT GCATGGCGTT TCAAAAAATA TCTCAAGTCA AAGAATTAGG CATTGATAAA 120 GCAAAGAGTT TGATAGGCAA CCTTTCTCAA GTGATTATCT ACCCCACAAA AGATACTGAT 180 GAATTAATAG AATGTGGCGT CCCATTAAGC GATAGTGAAA TCAATTTCTT ACACAACACG 240 GACATGAGAG CCAGACAAGT GCTAGTAAAA AATATCGTTA CAAACGCTTC AGCTTTTATT 300 GAAATTGATT TAAAAAAGAT TTGCAAGAAC TACTTTATAT TCTTGATAGC AATGCTGGTA 360 ATAGAAAAAT CCTCAATGAT CTTAAAAAAG CAAACCAAGA AACTTATAAG GAAGAGTATT 420 TAA 423 (2) INFORMATION FOR SEQ ID NO:13: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 771 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1. .771 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:13: ATGTTGGGGA GCGTCAAAAA AGCGGTTTTT AGGGTTTTGT GTTTGGGGGC GTTGTGTTTA 60 TGCGGGGGGT TAATGGCAGA GCAAGATCCT AAAGAGCTTA TATTTTCAGG TATAACTATT 120 TACACGGATA ARAATTTCAC TAGAGCTAAG AAATATTTTG AAAAAGCTTG CAAATCAAAC 180

GATGCTGATG GCTGTGCAAT CTTAAGAGAG GTTTATTCTA GTGGTAAAGC CATAGCGAGA 240 GAAAACGCAA GAGAGAGCAT TGAAAAAGCT CTTGAACACA CCGCTACTGC TAAAGTTTGT 300 AAATTAAACG ATGCTGAAAA ATGCAAGGAC TTAGCAGAGT TTTATTTTAA TGTAAACGAT 360 CTTAAAAATG CTTTAGAATA TTACTCTAAA TCTTGTAAGT TAAATAATGT TGAAGGGTGT 420 ATGCTGTCAG CAACTTTTTA TAACGATATG ATAAAGGGTT TGAAAAAAGA TAAAAAAGAT 480 CTAGAATATT ATTCTAAAGC TTGCGAGTTA AATAACGGTG GAGGGTGTTC TAAATTAGGA 540 GGGGATTATT TTTTTGGTGA AGGCGTAACA AAAGATTTCA AAAAAGCTTT TGAATATTCT 600 GCCAAAGCTT GTGAGTTGAA CGATGCTAAA GGGTGTTACG CTCTAGCAGC GTTTTATAAT 660 GAGGGTAAAG GCGTGGCAAA GGATGAAAAG CAAACGACAG AAAACCTTGA AAAGAGTTGC 720 AAGCTAGGAT TAAAAGAAGC ATGCGATATT CTCAAAGAAC AAAAACAATA A 771 (2) INFORMATION FOR SEQ ID NO:14: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 729 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1. .729 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:14: ATGAAAAAAT TTTTTTCTCA ATCTTTGTTA GCTCTTATTA TCTCTATGAA TGCGGTATCT 60 GGCATGGATG GTAATGGCGT TTTTTTAGGG GCGGGTTATT TGCAAGGACA GGCGCAAATG 120 CATGCGGATA TTAATTCTCA AAAACAAGCC ACCAACGCTA CGATCAAAGG CTTTGACGCG 180 CTCTTGGGGT ATCAATTTTT CTTTGAAAAA CACTTTGGCT TACGCCTTTA TGGGTTTTTT 240 GACTACGCTC ATGCCAATTC TATTAAGCTT AAAAACCCTA ACTATAATAG CGAAGCGGCG 300 CAAGTGGCTA GTCAAATTCT TGGGAAACAA GAAATCAATC GTTTAACAAA CATTGCCGAT 360 CCCAGAACTT TTGAGCCGAA CATGCTCACT TATGGGGGGG CTATGGACGT GATGGTTAAT 420 GTCATCAATA ACGGCATCAT GAGTTTGGGG GCTTTTGGCG GGATACAATT GGCCGGCAAT 480 TCATGGCTTA TGGCGACACC GAGCTTTGAG GGCATTTTAG TGGAACAAGC CCTTGTGAGC 540 AAGAAAGCCA CTTCTTTCCA ATTTTTATTC AATGTGGGGG CTCGCTTAAG GATCTTAAAA 600 CATTCTAGCA TTGAAGCGGG CGTGAAATTC CCCATGCTAA AGAAAAACCC CTACATCACT 660 GCAAAAAATT TGGATATAGG GTTTAGGCGC GTGTATTCGT GGTATGTGAA TTACGTGTTC 720 ACTTTCTAG 729 (2) INFORMATION FOR SEQ ID NO:15: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 804 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular

(ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...804 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:15: ATGAACTACC CTAATCTACC TAACAGCGCT TTAGAGATAA GCGAACAGCC AGAAGTGAAA 60 GAAATCACTA ACGAGCTTTT AAAGCAATTA CAAAACGCTT TAAGGAGCAA CGCGCATTTT 120 AGCGAGCAAG TGGAATTAAG CCTTAAATGC ATCGTTAGGA TTTTAGAAGT GCTTTTGAGT 180 TTGGATTTTT TTAAGAATGC GAATGAGATT GATAGCAGTT TAAGAAATTC CATTGAGTGG 240 CTGACTAACG CCGGCGAGAG CTTGAAATTA AAAATGAAAG AATACGAGCG CTTTTTTAGC 300 GAGTTTAATA CGAGCATGCA TGCCAACGAG CAGGAAGTAA CCAATACCTT AAACGCTAAC 360 GCCGAGAACA TTAAAAGCGA AATTAAAAAG CTAGAAAATC AATTGATAGA AACCACGACA 420 AGACTTTTAA CGAGCTATCA AATCTTTTTA AACCAAGCCA GAGATAACGC TAACAACCAA 480 ATCACAAAAA ACAAAACCCA AAGCCTTGAA GCGATTACAC AAGCTAAAAA CAACGCTAAT 540 AATGAAATAA GCAACAATCA AACGCAAGCG ATAACTAATA TCACCGAAGC GAAAACGAAC 600 GCTAATAATG AAATAAGCAA CAATCAAACG CAAGCGATAA CTAACATTAA CGAAGCCAAA 660 GAAAGCGCTA CAACGCAAAT AAACGCCAAT AAGCAAGAAG CAATAAATAA CATCACGCAA 720 GAAAAAACCC AAGCCACAAG CGAGATCACC GAAGCGAAAA AGACCGATCA TTATCAAAAC 780 ATTGATTTTT TTGAGTTTGA ATAA 804 (2) INFORMATION FOR SEQ ID NO:16: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1632 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...1632 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:16: GTGATAGAGA CCATCCCCAA ACACTCTAAG ATTGTTTTAC CCGGGGAGGC GTTTGATAGT 60

TTAAAAGAGG CGTTTGATAA AATTGACCCC TATACTTTCT TTTTTCCAAA ATTTGAAGCC 120 ACTAGCACTT CTATTTCTGA TACTAACACG CAGAGGGTGT TTGAAACGCT CAATAACATT 180 AAAACAAATC TTATAATGAA ATATAGTAAT GAAAATCCAA ACAATTTCAA CACTTGTCCT 240 TACAATAATA ATGGTAATAC AAAAAATGAT TGTTGGCAAA ATTTCACCCC ACAAACCGCA 300 GAAGAATTCA CCAATTTAAT GTTGAACATG ATCGCTGTCT TAGACTCCCA ATCTTGGGGC 360 GATGCGATCT TAAACGCTCC TTTTGAATTC ACTAACAGCT CAACAGATTG CGATAGCGAT 420 CCTTCAAAAT GCGTAAATCC CGGAGTAAAT GGGCGTGTTG ATACTAAAGT CGATCAACAA 480 TATATACTCA ACAAACAAGG TATTATTAAT AATTTTAGAA AAAAAATAGA AATTGATGCG 540 GTTGTTTTAA AAAATTCAGG GGTTGTAGGG TTAGCCAATG GATATGGCAA TGATGGTGAA 600 TATGGCACAT TAGGGGTAGA AGCCTATGCT TTAGATCCTA AAAAACTCTT TGGCAACGAC 660 CTTAAGACTA TCAATTTAGA AGATTTAAGA ACCATCTTGC ATGAATTCAG CCACACTAAA 720 GGCTATGGGC ATAACGGGAA TATGACCTAT CAAAGAGTGC CGGTAACGAA AGATGGTCAA 780 GTGGAAAAGG ATAGTAATGG CAAGCCAAAA GATTCTGATG GCCTCCCCTA TAATGTGTGT 840 TCGCTTTATG GGGGATCCAA TCAGCCCGCT TTCCCTAGCA ACTACCCTAA TTCCATCTAT 900 CACAATTGTG CGGATGTCCC GGCTGGCTTT TTAGGGGTAA CAGCAGCGGT TTGGCAGCAG 960 CTCATCAATC AAAACGCCTT GCCGATCAAC TACGCTAACT TGGGGAGTCA AACAAACTAC 1020 AACCTAAACG CTAGTTTAAA CACGCAAGAT TTAGCCAATT CCATGCTCAG CACCATCCAA 1080 AAAACCTTTG TAACTTCTAG CGTTACCAAC CACCATTTTT CAAACGCATC GCAAAGTTTT 1140 AGAAGCCCTA TTTTAGGGGT TAACGCTAAA ATAGGCTATC AAAACTACTT TAATGATTTC 1200 ATAGGGTTGG CTTATTATGG CATCATCAAA TACAATTACG CTAAAGCTGT TAATCAAAAA 1260 GTCCAGCAAT TGAGCTATGG TGGGGGGATA GATTTGTTAT TGGATTTCAT CACCACTTAC 1320 TCCAATAAAA ATAGCCCTAC AGGCATTCAA ACCAAAAGGA ATTTTTCTTC ATCTTTTGGT 1380 ATCTTTGGGG GGTTAAGGGG CTTGTATAAC AGCTATTATG TGTTGAACAA AGTCAAAGGA 1440 AGCGGCAATT TAGATGTGGC TACCGGGTTG AACTACCGCT ATAAGCATTC TAAATATTCT 1500 GTAGGGATTA GCATCCCTTT AATCCAAAGA AAAGCTAGCG TCGTTTCTAG CGGTGGCGAT 1560 TATACGAACT CTTTTGTTTT CAATGAAGGG GCTAGCCACT TTAAGGTGTT TTTCAATTAC 1620 GGTGGGTGTT TT 1632 (2) INFORMATION FOR SEQ ID NO:17: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1071 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...1071 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:17: TTGATGAAAA GCATTTTGCT CTTTATGATT TTTGTAGTTT GTCAGTTAGA AGGCAAAAAA 60 TTTTCACAAG ATAATTTTAA GGTGGATTAT AACTACTATT TGCGCAAACA GGATTTGCAC 120 ATCATTAAAA CGCAAAACGA TTTGTCCAAT GCCTGGTATC TCCCTCCACA AAAAGCCCCC 180 AAAGAACATT CTTGGGTGGA TTTTGCTAAA AAATATTTAA ACATGATGGA TTATCTAGGC 240

ACTTATTTTT TGCCTTTTTA TCATAGTTTC ACCCCCATTT TTCAATGGTA CCACCCTAAT 300 ATCAACCCCT ACCAACGCAA TGAGTTTAAG TTCCAAATCA GTTTTAGAGT GCCTGTATTT 360 AGGCATATTC TTTGGACTAA AGGCACGCTT TATCTGGCTT ATACCCAAAC TAACTGGTTT 420 CAAATTTATA ATGACCCTCA ATCCGCCCCC ATGCGAATGA TCAATTTCAT GCCTGAACTC 480 ATCTATGTTT ATCCTATTAA TTTTAAACCT TTTGGGGGTA AAATAGGGAA TTTTTCTGAA 540 ATTTGGATAG GTTGGCAGCA CATTTCTAAT GGTGTGGGGG GTGCGCAATG TTACCAGCCT 600 TTTAATAAAG AAGGTAATCC TGAAAACCAG TTTCCAGGAC AACCTGTAAT CGTTAAAGAT 660 TATAACGGGC AAAAAGATGT GCGCTGGGGG GGGTGTCKTT CGGTGARCSC GGGCAACSCC 720 CTGTGTTTCG TTTTGGTGTG GGAAAAGGGA GGCCTAAAAA TCATGGTCGC TTATTGGCCC 780 TATGTCCCTT ATGATCAATC CAACCCTCAA TTGATTGATT ACATGGGGTA TGGTAACGCT 840 AAAATTGATT ACAGGAGAGG GCGCCACCAT TTTGAATTGC AACTTTATGA TATTTTCACG 900 CAATACTGGC GTTATGATCG CTGGCATGGA GCTTTCCGCT TAGGCTATAC CTACCGCATT 960 AACCCTTTTG TGGGGATTTA TGCGCAGTGG TTTAACGGCT ATGGCGATGG CTTGTATGAA 1020 TACGATGTTT TTTCCAATCG TATAGGGGTA GGAATACGCT TGAACCCTTA A 1071 (2) INFORMATION FOR SEQ ID NO:18: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 2028 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...2028 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:18: TTGTCTAAAG GTTTGAGTAT CGGTAATAAA ATCATATTGT GCGTGGCGTT GATTGTGATC 60 GTGTGCGTGA GCATTTTAGG GGTGTCCTTA AACAGCAGGG TGAAAGAGAT TTTAAAAGAA 120 AGCGCTCTGC ATTCAATGCA AGATAGTTTG CATTTCAAGG TTAAGGAAGT GCAAAGTGTT 180 TTGGAAAACA CTTATACGAG CATGGGCATT GTCAAAGAAA TGCTCCCTGA AGACACCAAA 240 AGAGAAATCA AAATCCAGTT GTTAAAAAAC TTCATTTTAG CCAATTCGCA TGTCGCTGGG 300 GTGAGCATGT TTTTTAAAGA CAGAGAGGAT TTGAGATTGA CGCTTTTACG AGATAACGAT 360 ACGATCAAGT TGATGGAAAA CCCGTCATTA GGGAGTAACC CTTTAGCGCA AAAAGCGATG 420 AAAAATAAAG AAATTTCTAA AAGCTTGCCT TATTACAGGA AAATGCCTAA CGGGGCGGAA 480 GTTTATGGCG TGGATATTCT TTTACCACTA TTCAAGGAAA ACACGCAAGA AGTGGTGGGG 540 GTTCTGATGA TTTTCTTTTC CATTGACAGC TTCAGTAATG AAATCACTAA AAACAGGAGC 600 GATTTATTTT TAATTGGCGT TAAAGGTAAA GTGCTTTTGA GCGCGAATAA AAGCTTGCAA 660 GACAAATCCA TCACCGAAAT TTATAAAAGC GTGCCTAAAG CCACTAATGA AGTGATGGCT 720 ATTTTAGAAA ATGGCTCTAA AGCGACTTTA GAATACTTGG ATCCCTTTAG CCATAAGGAG 780 AATTTTTTAG CCGTTGAAAC CTTTAAAATG CTAGGCAAAA CAGAAAGTAA AGACAATCTT 840 AATTGGATGA TCGCTTTGAT CATTGAAAAA GACAAGGTCT ATGAGCAAGT GGGATCGGTG 900 CGTTTTGTGG TGGTTGCAGC GAGTGCTATC ATGGTGTTAG CCTTAATCAT AGCGATCACT 960 CTTTTAATGC GAGCGATCGT GAGCAATCGT TTGGAAGTCG TTTCTAGCAC CTTGTCTCAT 1020

TTCTTTAAAT TATTGAACAA TCAAGCCCAT TCTAGCGACA TTAAATTGGT TGAAGCGCGA 1080 TCTAATGACG AATTAGGGCG CATGCAAACA GCGATCAATA AAAATATCTT GCAAACCCAA 1140 AAAACCATGC AAGAAGACAG GCAAGCCGTC CAAGACACCA TTAAAGTGGT TTCAGACGTG 1200 AAAGCGGGGA ATTTTGCGGT GCGCATCACG GCTGAACCCG CAAGCCCTGA TTTGAAAGAA 1260 TTGAGAGACG CGCTAAATGG GATCATGGAT TATTTGCAAG AAAGCGTAGG GACTCACATG 1320 CCAAGCATTT TCAAAATCTT TGAAAGCTAT TCTGGCTTGG ATTTTAGAGG GCGGATCCAA 1380 AACGCTTCGG GTAGGGTGGA ATTGGTTACT AACGCTTTAG GGCAAGAAAT CCAAAAAATG 1440 CTAGAAACTT CGTCTAATTT TGCCAAAGAT CTAGCGAACG ATAGCGCGAA TTTAAAAGAA 1500 TGCGTGCAAA ATTTAGAAAA GGCTTCAAAC TCCCAACACA AAAGCCTGAT GGAAACTTCC 1560 AAAACGATAG AAAATATCAC CACTTCCATT CAAGGCGTGA GCTCTCAAAG TGAAGCCATG 1620 ATTGAACAAG GGAAAGACAT TAAAAGCATT GTAGAAATCA TTAGAGATAT TGCCGATCAA 1680 ACGAATCTAT TAGCCCTAAA CGCTGCTATT GAAGCCGCAC GAGCCGGCGA GCATGGCAGA 1740 GGCTTTGCGG TGGTGGCTGA TGAGGTGAGG AAGCTCGCTG AAAGGACGCA AAAATCCCTC 1800 AGTGAGATTG AAGCCAATAT TAATATTCTC GTTCAAAGCA TTTCAGACAC GAGCGAAAGC 1860 ATTAAAAACC AGGTTAAAGA AGTAGAAGAG ATCAACGCTT CTATTGAAGC CTTAAGATCG 1920 GTTACTGAGG GCAATCTAAA AATCGCTAGC GATTCTTTAG AAATCAGTCA AGAAATTGAC 1980 AAAGTCTCTA ACGATATTTT AGAAGATGTG AATAAAAAGC AGTTTTAA 2028 (2) INFORMATION FOR SEQ ID NO:19: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 816 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...816 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:19: ATGAACATAT TCAAGCGTAT TATTTGCGTA ACCGCTATTG TTTTAGGTTT TTTTAACCTT 60 TTAGACGCCA AACACCACAA AGAAAAF1AAA GAAGACCACA AAATCACTCG TGAGCTTAAA 120 GTGGGCGCTA ACCCTGTGCC GCATGCGCAA ATCTTGCAAT CAGTTGTGGA TGATTTGAAA 180 GAGAAAGGGA TCAAATTAGT GATCGTGTCT TTTACGGATT ATGTGTTGCC TAATTTAGCG 240 CTCAATGACG GCTCTTTAGA CGCGAATTAC TTCCAGCACC GCCCTTATTT GGATCGGTTT 300 AATTTGGACA GAAAAATGCA CCTTGTTGGT TTGGCCAATA TCCATGTGGA GCCTTTAAGA 360 TTTTATTCTC AAAAAATCAC AGACATTAAA AACCTTAAAA AAGGCTCAGT GATTGCTGTG 420 CCAAATGATC CGGCCAATCA AGGCAGGGCG TTGATTTTAC TCCATAAACA AGGCCTTATC 480 GCTCTCAAAG ACCCAAGCAA TCTATACGCT ACGGAGTTTG ATATTGTCAA AAATCCTTAC 540 AACATCAAAA TCAAACCCCT AGAAGCTGCG TTATTGCCTA AGGTTTTAGG GGATGTGGAT 600 GGGGCTATCA TAACAGGGAA TTATGCCTTG CAAGCAAAAC TCACCGGAGC CTTATTTTCA 660 GAAGATAAGG ACTCGCCTTA TGCTAATCTT GTAGCCTCTC GTGAGGATAA TGCGCAAGAT 720 GAAGCGATAA AAGCGTTGAT TGAAGCCTTA CAGAGCGAAA AGACCAGGAA ATTCATTTTG 780 GATACCTATA AGGGGGCGAT TATCCCGGCT TTTTAA 816

(2) INFORMATION FOR SEQ ID NO:20: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 486 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...486 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:20: ATGTTTTTTA AAACTTATCA AAAATTACTG GGCGCGAGCT GTTTGGCGCT GTATTTAGTG 60 GGCTGTGGGA ATGGTGGTGG CGGTGAATCG CCGGTTGAGA TGATTGCAAA TAGCGAGGGT 120 ACGTTTCAAA TCGACTCCAA AGCAGATAGC ATTACTATTC AAGGCGTGAA GCTTAATAGA 180 GGTAATTGTG CTGTCAATTT TGTTCCAGTA AGTGAGACGT TTCAAATGGG TGTTTTAAGT 240 CAAGTTACTC CAATCTCTAT ACAGGATTTT AAAGATATGG CAAGCACTTA TAAGATATTT 300 GATCAAAAGA AAGGGTTGGC AAACATAGCA AATAAAATTT CTCAATTAGA GCAAAAGGGT 360 GTGATGATGG AACCTCAAAC CCTTAATTTT GGAGAAAGTT TAAAAGGCAT TTCTCAAGGG 420 TGCAATATTA TAGAGGCAGA AATACAAACC GACAAAGGCG CTTGGACTTT TAACTTTGAT 480 AAATAA 486 (2) INFORMATION FOR SEQ ID NO:21: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1014 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...1014

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:21: ATGATTAGAT TAAAAGGTTT GAATAAAACT TTAAAAACAA GCTTATTAGC TGGGGTTTTA 60 CTAGGTGCTA CTGCTCCCTT AATGGCAAAG CCTTTATTAA GCGATGAAGA CTTATTGAAA 120 CGAGTAAAAC TACACAATAT CAAAGAAGAT ACGCTGACTA GCTGTAATGC TAAGGTGGAC 180 GGCTCTCAAT ACTTGAATAG TGGTTGGAAT TTATCTAAAG AATTTCCGCA AGAATATAGA 240 GAAAAGATTT TTGAATGCGT AGAAGAAGAA AAACATAAAC AAGCCCTTAA TTTAATCAAT 300 AAAGAAGACA CTGAAGATAA AGAAGAACTT GCAAAA AAA TCAAAGAAAT TAAAGAAAAA 360 GCTAAAGTTT TAAGGCAAAA ATTTATGGCT TTTGAAATGA AAGAACACTC TAAAGAATTC 420 CCAAATAAAA AGCAACTTCA AACCATGCTT GAGAACGCTT TTGATAATGG AGCTGAAAGT 480 TTTATTGATG ATTGGCACGA ACGCTTTGGG GGTATAAGTA GAGAGAATAC TTATAAAGCA 540 CTTGGCATTA AAGAATATAG TGATGAAGGA AAGATATTAG CCTTTGGCGA AAGAAGTTAT 600 ATTAGACAAT ATAAAAAAGA TTTTGAAGAA AGCACTTATG ATACTAGACA AACCTTATCT 660 GCTATGGCTA ATATGAGTGG CGAAAACGAT TATAAAATTA CTTGGTTAAA ACCCAAATAT 720 CAGCTCCATA GTTCAAATAA TATTAAACCC TTAATGTCAA ACACAGAGTT GTTAAATATG 780 ATAGAGCTAA CCAATATCAA AAAAGAATAT GTTATGGGCT GTAATATGGA AATAGATGGT 840 TCTAAATATC CCATTCATAA AGATTGGGGA TTTTTTGGTA AGGCAAAAGT CCCAGAAACT 900 TGGAGAAATA AGATTTGGGA ATGTATTAAG AATAAAGTAA AGTCCTATGA CAACACTACC 960 GCTGAAATAG GAATAGTTTG GAAAAAAAAT ACTTATTCTA TCTCTCATCA CTAA 1014 (2) INFORMATION FOR SEQ ID NO:22: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1251 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1. .1251 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:22: ATGAAAAAAT TAGTTTTTAG CATGCTTTTA TGTTGTAAAA GCGTGTTTGC AGAGGGGGAA 60 ACTCCTTTGA TTGTCAATGA CCCAGAAACC CATGTAAGTC AAGCCACTAT CATAGGCAAA 120 ATGGTAGATA GTATCAAAAG ATACGAAGAG ATTATTTCTA AGGCTCAAGC TCAAGTCAAT 180 CAGTTACAAA AAGTCAATAA CATGATAAAT ACGACTAATT CTTTGATTAG TAGTAGTGCT 240 ATCACTTTAG CCAATCCTAT GCAAGTTTTA CAAAACGCTC AGTATCAAAT AGAGAGCATT 300 AGATACAACT ATGAGAATTT AAAGCAAAGC ATAGAAAATT GGAACGCACA AAATTTGTTA 360 AGAAACAAAT ACTTACAGCA ACAATGCCCT TGGCTTAATG TCAATGCTCT TACTAACAAT 420 AAGATTGTCA ATCTTAAAGA TCTCAATAAC CTAATCACCA AARATGGCGA ACAAACCCAA 480 ACCGCAAGAG ATGTGCAAAA TCTCATTCAG TCCATTAGTG GCAGTGGCTA TGGAAACATG 540 CAATCACTTG CTGGGGAATT GAGTGGTAGA GCGTGGGGGG AAATGTTGTG TAAAATGGTA 600 AACGATAGTA ATTATGAAAG CGAGCAAGCT CTTTTAGCAA CAGGCAATAA CCCAGAAGAG 660 CAAAAACGAA GATTTTTGCT TAGAGTAAAG ARAAAGGTTA ATGATAATAA GCAGTTAAAA 720

GATAAACTTG ACCCATTTCT AAAAAGACTT GATGTCCTAC AAACTGAGTT TGGTGTAACT 780 GACCCTACAG CTAACCATAA TAAGCAAGGG ATACATTATT GCACAGAAAA TAAAGAGACA 840 GGTAAATGCG ACCCTATTAA AAATGTATTT AGGACAACTC GCTTAGATAA CGAATTAGAA 900 CAAGAAATCC AAACGCTCAC ACTTGATTTA ATCAAAGCCT CCAATAAAGA CGCTCAAAGC 960 CAAGCCTACG CAAATTTCAA TCAAAGGATT AAATTACTTA CTCTAAAATA TTTAAAAGAA 1020 ATTACCAATC AAATGCTCTT TTTAAATCAA ACAATGGCAA TGCAAAGCGA GATTATGACA 1080 GATGATTATT TTAGGCAAAA TAATGATGGC TTTGGGGAAA AAGAAAACCA TATAGACAAA 1140 CAATTAACGC AAAAAAGAAT AAACGAAAGA GAAAGAGCTA GAATATACTT TCAAAACCCT 1200 AATGTTAAAT TTGACCAATT TGGCTTTCCC ATTTTTAGTA TATGGGATTA A 1251 (2) INFORMATION FOR SEQ ID NO:23: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1131 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1. .1131 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:23: GTGAATAAGT GGATTAAAGG GGCGGTTGTT TTTGTAGGGG GTTTTGCAAC GATTACAACC 60 TTTTCTTTAA TCTACCACCA AAAGCCAAAA GCCCCCCTAA ATAACCAGCC TAGCCTTTTG 120 AATGACGATG AGGTGAAATA CCCCTTACAA GACTACACTT TCACTCAAAA CCCACAGCCA 180 ACTAACACGG AAAGCTCCAA AGACGCTACC ATCAAAGCCT TACAAGAACA GCTCAAAGCC 240 GCTTTAAAAG CCCTAAACTC CAAAGAAATG AATTATTCCA AAGAAGAGAC TTTTACTAGC 300 CCTCCCATGG ATCCAAAAAC AACCCCCCCT AAAAAAGACT TTTCTCCAAA ACAATTAGAT 360 TTACTGGCCT CTCGCATCAC CCCTTTCAAG CAAAGCCCTA AAAATTACGA AGAAAACCTG 420 ATTTTCCCTG TGGATAACCC TAATGGCATT GATAGTTTCA CTAACCTTAA AGAAAAAGAC 480 ATCGCCACTA ATGAAAACAA GCTTTTACGC ACCATTACAG CTGACAAAAT GATACCCGCT 540 TTTTTGATTA CGCCCATTTC TAGCCAGATC GCTGGTAAAG TGATTGCGCA AGTGGAGAGC 600 GATATTTTTG CAAGCATGGG CAAAGCCGTC TTAATCCCCA AAGGCTCTAA AGTCATAGGC 660 TATTACAGCA ACAATAACAA AATGGGCGAA TACCGCTTGG ATATTGTATG GAGTCGAATC 720 ATCACTCCCC ATGGCATTAA TATCATGCTC ACTAACGCTA AAGGGGCGGA CATTAAAGGC 780 TATAACGGCT TAGTGGGGGA ATTGATTGAA AGGAATTTCC AACGCTATGG CGTGCCGTTA 840 CTGCTTTCTA CGCTCACTAA CGGCCTATTG ATTGGGATCA CTTCGGCTTT AAACAACAGA 900 GGCAATAAAG AAGAGGTGAC TAATTTCTTT GGGGATTATC TTTTATTGCA ATTGATGAGG 960 CAAAGCGGCA TGGGGATCAA TCAAGTGGTC AATCAAATTT TAAGAGACAA GAGCAAGATC 1020 GCCCCCATTG TGGTGATTAG AGAGGGGAGT AGGGTCTTCA TTTCGCCCAA TACTGACATC 1080 TTCTTCCCTA TACCCAGAGA GAATGAAGTC ATCGCTGAGT TTTTGAAGTG A 1131 (2) INFORMATION FOR SEQ ID NO:24:

(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 2751 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...2751 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:24: GTGGATTTGA GGATCCAATC TAAAGAAGTC AGTCATAATT TAAAGGAATT ATCAAAAACG 60 CTAATCAGCT ATCCTTTTGA AAAACATGTA GAAGCTTTAG GGGAACAATG CAGTAACTTC 120 GTTTCTATTC CCATTAACAA TGACGACTAT TCAAATATTT GCACTTTTGT GAGTGATTTT 180 ATAAATCTTA TAGCTTCTTA CAATTTATTA GAATCATTTT TAGATTTTTA TAAAGATAAA 240 TTAAAATTGA GCGAGCTTGT AACTGAATAT GCCAACGTAA CCAATAATCT GCTTTTCAAA 300 AAATTAATCA AACATTTAAG CGGCAACAAT CAATTGGTTA AAAATTTTTA TCAGTGTATA 360 AGAGAAATTA TAAAATACAA CGCCCCTAAT AAAGAATACA AACCCAATCA ATTTTTTATA 420 ATAGGGAAAG GCAAACAAAA ACAATTAGCA AAAATTTATT CTCATTTAAA AGAACTTAGT 480 GCAAGTGAAA TTAAACCACA AGATATGGAA GACATCTTAA AAAAGCTAGA GGAATTAGAT 540 AAAATTTTTA AAACTACCGA CTTTACAAAA TTCACACCAA AAACTGAAAT TAAGGATATT 600 ATTAAAGAAA TAGACGAAAA ATACCCTATC AATGAAAATT TTAAACGGCA ATTTAATGAG 660 TTTGAATCAA ATATTGAAAA ACATGATGAA ATAAAAAAGG ATTTTGAGCG AAACAAAGAG 720 TCGCTGATCC GAGAAATTGA AAATCACTGC AAAAATGAAT GCAATAGCGA AGAAGAGCCG 780 GAGTATAAGA TTAATGATCT GCTCAAAAAT ATCCAACAAA TATGCAAAAA TTATATAGAA 840 AGTCATGCCG TTAATGATGT GTCTAAAGAT ATTAAATCCA TGATGTGTCA GTTTTATTTG 900 AAACAGATAG ATTTATTAGT CAATTCAGAA ATTGTGCGAT ACAGATACAG CAATCTTTTT 960 GAACCAATAC AAAGATCTTT ATGGGAGAGT ATAAAAATTT TAGATAATGA AAGTGGCATT 1020 TATTTGTTCC CTAAAAATAT TGGTGAAATC AAGGATAAAT TTGAAGCAAA CAAGGAAAAA 1080 TTCAAACAAA GCAAAAATGT TTCTGAGTTC GCAGAATATT GCCGAGAGTG TAACCCCTAT 1140 ACAGCGTTTA ACTTTCATCT AAATATAAAT AATGGTTTAT CTCATCAATT TGAAAAATTC 1200 GTGCCAATCA TGAAAGAATA CAAAGAGCCA AAAATCACAG ATAATGACCT TGAAGCCATA 1260 TCAACCAAAG AGACTGGTCT TGCTAGCCAA TTATCTGGGC ACTGGTTTTT TCAGCTTTCG 1320 TTATTTAATA AAACAAACTT TAATCCTAAT AAAATTTGGA TTCCTTTAGA GTTCAATAAA 1380 AGATCAAAAA TAAAGTTTGA TAAAGATTTA GAAATCTATT TTGATAGTCA TGAATCGTTC 1440 AATATCTCTA AAAAATACTT GCAAGAAATA GATCAAGAAT CACTAAAAA GATCAAACAA 1500 TCAAAAGATT TTTTTTCAAT TCAAAAAATA GAGAGTAAGC ATGATAATAA CGATATACTG 1560 CAACTTGAAT TTTTTGAGAA TGATACAAGT TTTCTTTTTG CTAAAGGAAG TTTTGCAGAA 1620 ATTTTAGAAT ACAACATGCA ATTAAAAATA GATTCTTTAA TTACAAAAGA ATTTAATAAG 1680 CTTTTAGCGA TCGTTCAAGA TAGTCCCCAA GATAGTTACC AATTAAAAAT TCGTGTCCGA 1740 CATAACAATA AGCTTCCTAG AGAGAAATAT ACGGAACATG AAATAAAACT TGAAGTTTAT 1800 GATTGCAGAA AATCCCACGA TCACAATGAG CCAATCATCT TAAGCCAGCA AAGCACCGGC 1860 TTCCAATGGG CGTTTAATTT CATGTTTGGC TTTCTTTATA ATGTGGGATC ACATTTTAGT 1920 TTTAACCATA ATATTATCTA TGTCATGGAC GAGCCAGCCA CTCATTTGAG CGTGCCAGCC 1980 AGAAAGGAGT TTAGGAAATT TTTAAAAGAA TACGCTCATA AAAATCATGT TACTTTTGTT 2040

TTAGCCACCC ATGACCCCTT TTTAGTGGAT ACGGATCATT TAGATGAAAT AAGGATTGTG 2100 GAAAAGGAAA CAGAAGGCTC TGTAATTAAG AATCACTTTA ACTATCCCCT AAATAATGCA 2160 AGCAAAGACT CCGACGCTTT GGACAAAATC AAACGCTCTT TAGGAGTGGG CCAGCATGTT 2220 TTTCATAACC CCCAAAAACA CCGAATCATT TTTGTAGAAG GCATCACGGA TTATTGTTAT 2280 TTGAGCGCTT TTAAATTGTA TTTGCGTTAC AAAGAATACA AGGACAACCC CATTCCTTTC 2340 ACTTTCTTAC CCATTTCAGG GCTTAAAAAC GATTCAAACG ATATGAAAGA AACCATTGAA 2400 AAACTTTGCG AGTTAGACAA TCACCCTATT GTTTTGACAG ACGATGACAG AAAATGCGTT 2460 TTTAACCAAC AAGCAACGAG CGAACGATTT AAAAGAGCTA ATGAAGAAAT GCATGATCCC 2520 ATCACCATCC TACAACTCTC AGACTGCGAT AGGCATTTCA AACAAATTGA AGATTGTTTC 2580 AGCGCAAACG ATAGAAACAA ATACGCTAAA AATAAGCAAA TGGAATTGAG CATGGCTTTT 2640 AAAACAAGGC TTTTGTATGG CGGAGAAGAT GCGATAGAAA AACAAACAAA AAGAAATTTT 2700 TTAAAATTAT TCAAATGGAT TGCATGGGCT ACAAACTTGA TCAAAAACTA A 2751 (2) INFORMATION FOR SEQ ID NO:25: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 531 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...531 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:25: ATGACTGCAA TGATGCGTTA TTTTCACATC TATGCGACCA CTTTTTTCTT CCCTTTGGCG 60 CTTCTTTTTG CGGTTAGTGG GCTTTCATTG CTCTTTAAAG CGCGCCAAGA CACTGGCGCT 120 AAGATCAAAG AATGGGTTTT AGAAAAATCC TTAAAAAALG AAGAACGATT GGACTTTTTA 180 AAAGGCTTTA TAAAAGAAAA CCATATCGCT ATGCCTAAAA AGATAGAGCC TAGAGAGTAT 240 AGGGGAGCGT TAGTCATTGG CACGCCTTTG TATGAAATCA ACCTTGAAAC TAAAGGCACT 300 CAAACGAAAA TCAAGACCAT TGAAAGGGGC TTTTTAGGCG CGCTCATCAT GCTGCATAAG 360 GCTAAGGTGG GCATCGTGTT TCAGGCGCTT TTAGGGATTT TTTGCGTGTT TTTATTGTTG 420 TTTTACTTGA GCGCGTTTTT AATGGTGGCT TTTAAAGACA CTAAACGCAT GTTTATAAGC 480 GTTTTAATAG GGAGCGTGGT GTTCTTTGGA GCGATCTATT GGTCTTTGTA G 531 (2) INFORMATION FOR SEQ ID NO:26: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 669 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic)

(iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...669 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:26: ATGTTTAAAA ACGCTTTAAA TATACAAGAT TTTTCATTTA AAAATCATAC TAGTACAGCC 60 ATTATTGGCA CAAATGGTGC TGGAAAATCA ACGCTTATCA ACACTATTCT AGGCATTAGA 120 TCAGACTATA ATTTTAAAGC ACAAAACAAT AATATTCCAT ACCACGACAA TGTTATACCA 180 CAACGCAAGC AATTGGGAGT TGTCTCTAAC CTATTCAACT ACCCACCTGG ATTAAACGCA 240 AACGACCTTT TTAAATTCTA TCAATTTTTT CACAAAAACT GCACTCTAGA TTTGTTTGAA 300 AAAAATCTTT TAAATAAAAC CTACGAACAC CTAAGCGACG GACAAAAACA GCGCTTAAAA 360 ATTGACTTAG CTCTTAGCCA TCACCCACAA TTAGTTATTA TGGATGAACC AGAAACCAGT 420 TTAGAGCAAA ACGCTCTTAT AAGACTATCA AATCTCATAA GCTTGCGCAA CACCCAACAA 480 CTTACAAGTA TCATCGCCAC TCATGATCCT ATTGTCTTAG ATAGTTGCGA ATGGGTATTG 540 CTCCTTAAGA ATGGCAACAT TGCTCAATAC AAACCTTTAA ATTCTATATT AAAATCTGTA 600 GCTAAAACTT TTAACTTTAA AGAAAAACCA ACCACAAAAG ACTTATTAGC GTTACTAAAG 660 GATATTTAA 669 (2) INFORMATION FOR SEQ ID NO:27: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1221 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...1221 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:27: ATGTATGCGG CTCATCCTAT TAAACCCATA AAAGCCCCTA AACTCAAATC TCAATTTTTA 60 AGGCGTGTGT TTGTGGGCGC GTCCATTAGG CGCTGGAATG ACCAAGCATG CCCTTTGGAA 120 TTTGTGGAAT TAGACAAGCA AGCCCATAAA GCGATGATTG CGTATCTGCT CGCTAAAGAT 180 TTAAAAGATA GGGGTAAAGA TTTAGATTTA GATCTTTTAA TCAAATATTT TTGCTTTGAG 240 TTTTTGGAGC GCTTGGTTTT AACCGATATT AAACCCCCTA TTTTTTACGC CCTCCAACAA 300

ACGCATAGTA AAGAGTTAGC TTCCTATGTT GCGCAAAGTT TGCAAGATGA AATCAGTGCG 360 TATTTTTCTT TAGAGGAACT CAAAGAGTAT TTAAGCCACA GGCCTCAAAT TTTAGAAACT 420 CAAATTTTAG AGAGCGCGCA TTTTTATGCG TCTAAGTGGG AGTTTGATAT TATCTATCAT 480 TTTAACCCCA ACATGTATGG CGTGAAAGAG ATTAAAGATA AAATTGACAA GCAACTCCAC 540 AATAACGATC ATTTGTTTGA AGGGCTTTTT GGGGAAAAAG AAGATTTGAA AAAATTGGTG 600 AGCATGTTTG GGCAGTTGCG TTTCCAAAAG CGCTGGAGCC AAACCCCAAG AGTGCCACAA 660 ACCAGTGTTC TAGGGCATAC TTTATGCGTG GCGATTATGG GGTATTTATT GAGTTTTGAC 720 TTGAAAGCTT GTAAAAGCAT GCGGATCAAT CATTTTTTGG GCGGGCTTTT CCATGATTTA 780 CCCGAAATTT TAACCCGAGA CATTATCACG CCCATCAAAC AAAGCGTTGC AGGGCTTGAT 840 CATTGCATTA AAGAGATTGA AAAAAAGGAA ATGCAAAACA AAGTCTATTC CTTTGTGTCT 900 TTGGGCGTTC AAGAAGATTT GAAATATTTC ACCGAAAACG AGTTTAAAAA CCGCTACAAA 960 GACAAGTCTC ATCAAATCGT TTTCACTAAA GACGCTGAAG AATTATTCAC GCTTTATAAT 1020 AGCGATGAAT ATCTTGGGGT TTGCGGGGAG CTTTTGAAGG TGTGCGATCA TTTGAGCGCG 1080 TTTTTAGAAG CCCAAATCTC TCTTTCTCAT GGCATTTCTA GCTACGATTT AATCCAAGGA 1140 GCTAAAAACC TTTTAGAATT GCGATCCCAA ACGGAACTGC TTGATTTGGA TTTAGGGAAA 1200 TTGTTTAGAG ATTTTAAGTA A 1221 (2) INFORMATION FOR SEQ ID NO:28: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1008 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...1008 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:28: GTGTTGTGGG TGCTATATTT TTTAACCAGT TTATTTATTT GCTCTTTGAT TGTTTTGTGG 60 TCTAAAAAAT CCATGCTCTT TGTGGATAAC GCTAATAAAA TCCAAGGCTT CCATCATGCA 120 AGAACCCCAC GAGCCGGGGG GCTTGGGATC TTTCTTTCTT TTGCGTTGGC TTGTTATCTT 180 GAACCTTTTG AGATGCCTTT TAAGGGGCCT TTTGTTTTCT TAGGGCTATC GCTAGTGTTT 240 TTGAGCGGTT TTTTAGAAGA CATTAACCTT TCATTAAGCC CCAAAATACG CCTTATTTTG 300 CAAGCTGTAG GGGTCGTTTG CATCATTTCA TCAACGCCTT TAGTGGTGAG CGATTTTTCG 360 CCCCTTTTTA GCTTGCCTTA TTTCATCGCT TTTTTATTCG CTATTTTTAT GCTGGTGGGT 420 ATCAGTAACG CTATTAATAT CATTGACGGG TTTAACGGGC TTGCATCTGG GATTTGCGCG 480 ATCGCGCTTT TAGTCATTCA TTATATAGAC CCTAGCAGTT TGTCTTGTTT GCTCGCTTAC 540 ATGGTGCTTG GGTTTATGGT GTTAAATTTC CCTTCAGGAA AGATTTTTTT AGGCGATGGG 600 GGGGCGTATT TTTTGGGTTT GGTGTGCGGG ATTTCTCTCT TGCATTTGAG TTTGGAGCAA 660 AAAATCAGCG TGTTTTTTGG GCTCAATTTA ATGCTTTATC CGGTCATAGA GGTGCTTTTT 720 AGTATCCTTA GGCGCAAAAT AAAACGCCAG AAAGCCACCA TGCCGGATAA TTTGCATTTG 780 CACACCCTTT TATTTAAATT CTTGCAACAA CGCTCTTTCA ATTACCCTAA CCCTTTATGC 840 GCGTTTATCC TTATTCTATG CAACCTGCCT TTTATTTTAA TAAGCGTTTT GTTTCGCTTG 900

GACGCTTATG CGCTCATTGT GATTAGCCTA GTCTTTATCG CATGCTATTT AATAGGCTAT 960 GCTTATTTGA ATAGGCAAGT TTGCGCTTTA GAAAAGCGGG CGTTTTAA 1008 (2) INFORMATION FOR SEQ ID NO:29: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 291 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...291 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:29: ATGAAAAAGG TTATTGTGGC TTTAGGCGTT TTGGCGTTCG CAAATGTTTT AATGGCAACC 60 GATGTTAAGG CTCTTGTAAA AGGTTGTGCC GCTTGCCATG GGGTTAAGTT TGAAAAGAAA 120 GCTTTAGGTA AAAGCAAAAT CGTTAACATG ATGAGCGAAA AAGAGATTGA AGAGGATCTT 180 ATGGCTTTTA AAAGCGGTGC CAACAAGAAT CCTGTCATGA CCGCGCAAGC TAAAAAATTA 240 AGCGATGAAG ACATCAAAGC TTTAGCCAAA TACATCCCCA CTCTCAAATA A 291 (2) INFORMATION FOR SEQ ID NO:30: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 471 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...471 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:30:

ATGCGAGATT TCAATAACAT TCAAATCACA CGCTTAAAAG TGCGTCAAAA TGCCGTTTTT 60 GAAAAACTGG ATCTGGAGTT TAAAGATGGC TTGAGCGCGA TTAGTGGGGC TAGTGGGGTG 120 GGGAAAAGCG TCCTTATTGC GAGCCTTTTA GGGGCGTTTG GGCTTAAAGA GAGCAACGCT 180 TCAAACATTG AAGTGGAATT GATCGCGCCT TTTTTAGACA CGGAAGAATA CGGCATTTTT 240 AGAGAAGATG AGCATGAACC CTTAGTTATT AGCGTGATTA AAAAAGAAAA AACACGCTAT 300 TTTTTAAACC AAACAAGCCT ATCTAAAAAC ACGCTCAAAG CGTTATTAAA GGGGCTTATT 360 AAACGCTTAT CTAACGACAG ATTCAGCCAG AATGAACTCA ACGATATTTT AATGCTCTCC 420 TTATTAGATG GCTATATCCA AAATAAAAAT AGGCGTTTAG CCCCCTTTTA G 471 (2) INFORMATION FOR SEQ ID NO:31: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 357 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...357 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:31: GTGATGCTAA TGGCAATTTT TACCCCTTAT ATTCTTATTT TGAAAATGAT GAAAAAGTCT 60 ATGAGTTTAT TCGCCAATAT GGGGTTGGAG CAAATTTTTT GCAACAGAGA CATTAAAGAT 120 TTAAATGATT TTGTTTTTGG TATAGAAGTG GGGCTTGATA GCAATGCGAG AAAAAATCGT 180 AGCAGAAAGG CTATGGAAAA TCATCTTATC GGTCTTTTTG TCCAAGCTCA ATTAAATTTT 240 AAAGAACAAG TAGATATTAG AGAATTTGAG GATTTACGCC AGGCTTTTGG AAATGATACT 300 AAAAAATTTG ATTTTGTTAT TTTTAGCAAA GAGAAAACTT ATTTTCATAG AAGCTAA 357 (2) INFORMATION FOR SEQ ID NO:32: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1068 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori

(ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...1068 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:32: ATGAATATCA AAATTTTAAA AATATTAGTT GGAGGGTTAT TTTTTTTGAG CTTGAACGCC 60 CATTTATGGG GGAAACAAGA CAATAGCTTT TTAGGGATTG GTGAAAGAGC CTATAAAAGC 120 GGGAATTATT CTAAAGCGGC GTCTTATTTT AAAAAAGCAT GCAACGATGG GGTGAGTGAA 180 GGCTGCACGC AATTAGGAAT CATTTATGAA AACGGGCAAG GCACTAGAAT AGATTATAAA 240 AAAGCCCTAG AATATTATAA AACCGCATGC CAGGCTGATG ATAGGGAAGG GTGTTTTGGC 300 TTAGGGGGGC TTTATGATGA GGGTTTAGGC ACGGCTCAAA ATTATCAAGA AGCCATTGAC 360 GCTTACGCTA AGGCATGCGT TTTAAAACAC CCTGAGAGTT GCTACAATTT AGGCATCATT 420 TATGATAGAA AAATCAAAGG CAATGCCGCT CAAGCGGTTA CTTACTATCA AAAAAGCTGT 480 AATTTTGATA TGGCTAAGGG GTGTTATATT TTAGGCACTG CCTATGAAAA AGGCTTTTTA 540 GAAGTCAAAC AGAGCAACCA TAAAGCCGTT ATCTATTATT TGAAAGCGTG CCGATTGAAT 600 GAGGGGCAGG CTTGCCGAGC GTTAGGGAGT TTGTTTGAAA ATGGCGATGC AGGGCTTGAT 660 GAAGATTTTG AAGTGGCGTT TGATTATTTG CAAAAAGCTT GCGCTTTAAA CAATTCTGGT 720 GGTTGCGCGA GTTTAGGCTC TATGTATATG TTGGGCAGGT ATGTTAAAAA AGACCCCCAA 780 AAGGCTTTTA ACTATTTCAA GCAAGCATGC GATATGGGGA GCGCGGTGAG TTGCTCTAGG 840 ATGGGCTTTA TGTATTCGCA AGGGGACACT GTTTCAAAAG ACTTGAGGAA AGCCCTTGAT 900 AATTATGAAA GAGGTTGCGA TATGGGCGAT GAAGTGGGTT GCTTCGCTCT AGCGGGCATG 960 TATTACAACA TGAAAGATAA AGAAAACGCC ATAATGATTT ATGACAAGGG CTGTAAATTG 1020 GGCATGAAAC AGGCATGCGA AAATCTCACC AAACTCAGGG GGTATTAG 1068 (2) INFORMATION FOR SEQ ID NO:33: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 582 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...582 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:33: ATGAAAGAAA AAAACTTTTG GCCTTTAGGA ATCATGAGCG TGCTTATTTT TGGGCTTGGG 60 ATCGTGGTGT TTTTAGTGGT GTTTGCCCTA AAAAATTCGC CTAAAAATGA TTTAGTGTAT 120 TTCAAGGGTC ATAACGAAGT GGATTTAAAC TTTAACGCCA TGCTTAAAAC TTATGAAAAC 180 TTTAAATCCA ATTATCGTTT TTCAGTGGGT TTAAAGCCTC TTACCGAAAG CCCTAAAACC 240 CCCATTTTGC CCTATTTTTC TAAAGGCACG CATGGGGATA AAAAAATCCA AGAAAACCTT 300 TTAAACAACG CTTTGATTTT AGAAAAGTCC AACACGCTTT ATGCACAATT GCAACCGCTC 360

AAACCCGCTT TAGATTCGCC AAATATTCAA GTGTATTTAG CGTTCTATCC CAGCCAATCC 420 CAGCCCAGAT TATTAGGAAC GCTTGATTGT AAAAACGCAT GCGAACCTTT AAAATTTGAT 480 TTGTTAGAGG GCGATAAAGT GGGGCGCTAT AAGATCCTTT TTAAATTTGT TTTTAAAAAT 540 AAAGAAGAAT TGATTTTGGA GCAACTGGCT TTTTTTAAGT AG 582 (2) INFORMATION FOR SEQ ID NO:34: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 870 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...870 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:34: TTGGGTATCA ATATGTGTTC TAAAAAAATA AGAAATCTCA TTTTATGCTT TGGTTTTATT 60 TTAAGCTTGT GCGCTGAAGA AAATATCACC AAAGAAAACA TGACTGAAAC GAACACGACT 120 GAAGAAAACA CCCCTAAAGA CGCTCCCATT CTTTTGGAAG AAAAACGCGC CCAAACTCTA 180 GAGCTTAAAG AAGAAAATGA AGTGGCAAAA AAGATTGATG AAAAAAGCCT GCTTGAAGAA 240 ATCCATAAGA AAAAACGCCA GCTTTACATG CTCAAAGGGG AATTGCATGA AAAGAATGAA 300 TCCATCTTAT TCCAACAAAT GGCTAAAAAT AAGAGCGGCT TTTTTATAGG CGTGATCCTT 360 GGCGATATAG GGATTAACGC TAATCCTTAT GAGAAGTTTG AACTTTTAAG CAATATTCAA 420 GCTTCTCCCT TGCTGTATGG TTTAAGGAGC GGGTATCAAA AGTATTTCGC TAACGGGATT 480 AGCGCCTTAC GCTTTTATGG GGAATATTTA GGGGGGGCGA TGAAAGGGTT TAAAAGCGAT 540 TCTTTAGCTT CTTATCAAAC CGCAAGCTTG AATATTGATC TGTTGATGGA TAAGCCTATT 600 GACAAAGAAA AAAGGTTTGC GTTAGGGATA TTTGGAGGCG TTGGAGTGGG GTGGAATGGG 660 ATGTATCAAA ATTTAAAAGA GATTAGAGGG TATTCACAGC CTAACGCCTT TGGGTTGGTG 720 TTAAATTTAG GGGTGAGCAT GACGCTCAAC CTCAAACACC GCTTTGAATT AGCCCTAAAA 780 ATGCCTCCCT TAAAAGAAAC TTCGCAAACC TTTTTATATT ATTTTAAAAG CACTAATATT 840 TATTATATTA GTTACAACTA TTTATTGTAA 870 (2) INFORMATION FOR SEQ ID NO:35: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 2007 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO

(iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...2007 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:35: ATGAGAAAAC TATTCATCCC ACTTTTATTA TTCAGCGCTT TAGAAGCGAA CGAGAAAAAC 60 GGCTTTTTCA TAGAAGCCGG CTTTGAAACT GGGCTATTAG AAGGCACACA AACGCAAGAA 120 AAAAGACACA CCACCACAAA AAACACTTAC GCAACTTACA ATTATTTACC CACAGACACG 180 ATTTTAAAAA GAGCGGCTAA TTTATTCACC AATGCCGAAG CGATTTCAAA ATTAAAATTC 240 TCATCTTTAT CCCCTGTTAG AGTGTTGTAT ATGTATAATG GTCAATTAAC TATAGAAAAC 300 TTCTTGCCTT ATAATTTAAA TAATGTTAAG CTTAGTTTTA CAGACGCTCA AGGCAACACG 360 ATTGATCTAG GCGTGATAGA GACCATCCCC AAACACTCTA AGATTGTTTT ACCCGGGGAG 420 GCGTTTGATA GTTTAAAAGA GGCGTTTGAT AAAATTGACC CCTATACTTT ATTTCTTCCA 480 AAATTTGAAG CCACTAGCAC TTCTATTTCT GATACTAACA CGCAGAGGGT GTTTGAAACG 540 CTCAATAACA TTAAAACAAA TCTTATAATG AAATATAGTA ATGAAAATCC AAACAATTTC 600 AACACTTGTC CTTACAATAA TAATGGTAAT ACAAAAAATG ATTGTTGGCA AAATTTCACC 660 CCACAAACCG CAGAAGAATT CACCAATTTA ATGTTGAACA TGATCGCTGT CTTAGACTCC 720 CAATCTTGGG GCGATGCGAT CTTAAACGCT CCTTTTGAAT TCACTAACAG CTCAACAGAT 780 TGCGATAGCG ATCCTTCAAA ATGCGTAAAT CCCGGAGTAA ATGGGCGTGT TGATACTAAA 840 GTCGATCAAC AATATATACT CAACAAACAA GGTATTATTA ATAATTTTAG AAAAAAATA 900 GAAATTGATG CGGTTGTTTT AAAAAATTCA GGGGTTGTAG GGTTAGCCAA TGGATATGGC 960 AATGATGGTG AATATGGCAC ATTAGGGGTA GAAGCCTATG CTTTAGATCC TAAAAAACTC 1020 TTTGGCAACG ACCTTAAGAC TATCAATTTA GAAGATTTAA GAACCATCTT GCATGAATTC 1080 AGCCACACTA AAGGCTATGG GCATAACGGG AATATGACCT ATCAAAGAGT GCCGGTAACG 1140 AAAGATGGTC AAGTGGAAAA GGATAGTAAT GGCAAGCCAA AAGATTCTGA TGGCCTCCCC 1200 TATAATGTGT GTTCGCTTTA TGGGGGATCC AATCAGCCCG CTTTCCCTAG CAACTACCCT 1260 AATTCCATCT ATCACAATTG TGCGGATGTC CCGGCTGGCT TTTTAGGGGT AACAGCAGCG 1320 GTTTGGCAGC AGCTCATCAA TCAAAACGCC TTGCCGATCA ACTACGCTAA CTTGGGGAGT 1380 CAAACAAACT ACAACCTAAA CGCTAGTTTA AACACGCAAG ATTTAGCCAA TTCCATGCTC 1440 AGCACCATCC AAAAAACCTT TGTAACTTCT AGCGTTACCA ACCACCATTT TTCAAACGCA 1500 TCGCAAAGTT TTAGAAGCCC TATTTTAGGG GTTAACGCTA AAATAGGCTA TCAAAACTAC 1560 TTTAATGATT TCATAGGGTT GGCTTATTAT GGCATCATCA AATACAATTA CGCTAAAGCT 1620 GTTAATCAAA AAGTCCAGCA ATTGAGCTAT GGTGGGGGGA TAGATTTGTT ATTGGATTTC 1680 ATCACCACTT ACTCCAATAA AAATAGCCCT ACAGGCATTC AAACCAAAAG GAATTTTTCT 1740 TCATCTTTTG GTATCTTTGG GGGGTTAAGG GGCTTGTATA ACAGCTATTA TGTGTTGAAC 1800 AAAGTCAAAG GAAGCGGCAA TTTAGATGTG GCTACCGGGT TGAACTACCG CTATAAGCAT 1860 TCTAAATATT CTGTAGGGAT TAGCATCCCT TTAATCCAAA GAAAAGCTAG CGTCGTTTCT 1920 AGCGGTGGCG ATTATACGAA CTCTTTTGTT TTCAATGAAG GGGCTAGCCA CTTTAAGGTG 1980 TTTTTCAATT ACGGGTGGGT GTTTTAG 2007 (2) INFORMATION FOR SEQ ID NO:36: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 192 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular

(ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...192 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:36: ATGAATACAG AAATTTTAAC CATCATGTTA GTTGTCTCCG TGCTTATGGG ATTGGTAGGC 60 TTAATAGCGT TTTTATGGGG GGTTAAAAGC GGTCAGTTTG ACGATGAAAA ACGCATGCTT 120 GAAAGCGTGT TGTATGACAG CGCGAGCGAC TTGAACGAAG CGATTTTACA AGAAAAACGC 180 CAAAAGAATT AA 192 (2) INFORMATION FOR SEQ ID NO:37: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1221 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...1221 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:37: ATGGTATTTT TTCATAAGAA AATTATTTTA AATTTTATCT ATTCTTTAAT GGTTGCTTTT 60 TTATTCCATT TATCCTATGG GGTTCTTTTA AAAGCCGATG GAATGGCTAA AAAGCAAACT 120 CTTTTAGTGG GTGAAAGGCT TGTGTGGGAT AAGCTCACGC TGTTAGGGTT TTTAGAAAAA 180 AACCATATCC CCCAAAAACT CTACTACAAT TTGAGCTCTC AAGATAAAGA ATTGAGTGCT 240 GAAATCCAAA GCAATGTTAC CTACTACACT TTAAGAGATG CAAATAACAC GCTCATTCAA 300 GCCCTTATCC CTATTAGCCA GGATTTGCAA ATCCATATTT ACAAAAAAGG AGAGGATTAT 360 TTTTTAGACT TTATCCCCAT TGTTTTCACT CGTAAAGAAA GAACCCTCCT TCTTTCCTTA 420 CAAACTTCGC CCTATCAAGA TATTGTCAAA GCCACCAATG ACCCCCTTTT AGCCAACCAA 480 TTGATGAACG CGTATAAAAA AAGCGTGCCT TTTAAACGCC TAGTGAAAAA CGATAAAATC 540 GCTATCGTTT ATACAAGGGA TTATCGTGTG GGGCAAGCGT TTGGCCAGCC GACCATCAAA 600 ATGGCGATGG TTAGCTCTCG TTTGCACCAA TACTATCTTT TTTCCCATTC AAACGGGCGT 660 TATTACGATT CAAAAGCGCA AGAAGTGGCA GGGTTTTTAC TAGAAACCCC GGTGAAATAC 720

ACCCGCATTT CTTCGCCTTT TTCGTATGGG AGGTTCCATC CTGTTTTAAA AGTTAAACGG 780 CCTCATTACG GCGTGGATTA TGCGGCTAAA CATGGCAGTT TGATCCATTC TGCTTCAGAC 840 GGCCGTGTGG GTTTTATAGG GGTTAAGGCG GGTTATGGGA AGGTGGTTGA AATCCATTTG 900 AATGAATTGC GCTTGGTGTA TGCTCACATG AGCGCGTTCG CTAACGGATT AAAAAAAGGC 960 TCGTTCGTTA AAAAAGGGCA AATCATAGGA AGAGTGGGAA GCACGGGTTT AAGCACCGGG 1020 CCGCATTTGC ATTTTGGCGT GTATAAAAAC TCCCGCCCCA TTAATCCTTT AGGCTATATC 1080 CGCACCGCTA AAAGCAAGCT GCATGGCAAA CAAAGAGAGG TTTTTTTAGA AAAAGCTCAG 1140 TATTCTAAGC AAAAATTAGA AGAACTTTTT AAAACCCATT CTTTTGAAAA AAATTCATTT 1200 TATCTTTTAG AGGGTTTTTA A 1221 (2) INFORMATION FOR SEQ ID NO:38: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 891 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...891 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:38: TTGTTTTTAG TCAAAAAAAT AGGCGTGGTA ATAATGATTT TAGTCTGCTT TTTAGCTTGC 60 TCGCAAGAGA GCTTTATCAA AATGCAAAAA AAAGCCCAAG AGCAAGAAAA TGACGGCTCT 120 AAACGCCCCA GCTATGTGGA TTCGGATTAT GAAGTCTTTA GCGAAACGAT TTTTTTACAA 180 AACATGGTGT ATCAGCCTAT AGAGGAAAGA AACGCTTTTT TCCAACTGAC TAAAGATGAA 240 GACAATTCTT TTAACCCTGA AAATTCCGTG ATTTTACTGA ATGAGCCAAG CGATAATAGT 300 GAAAAAAACC TACTCTCATA CCCAAACGAT CCCAATAACA ATGAAGACAA CGCTAATAAT 360 AGTCAAAAAA ATCCGTTCCT TTACAAGCCC AAAAGAAAAA CAAAAAACCC AAAACTCATT 420 GAATATTCCC AACAAGATTT CTACCCCCTA AAAAATGGGG ATATTATCAT GAGTAAAGAA 480 GGGGATCAAT GGTTGATAGA AATCCAATCC AAAGCCTTGA AGCGTTTTTT AAAAGATCAA 540 AACGATAAAG ATCGCCAGAT CCAAACTTTC ACTTTTAATG ACACTAAAAC GCAAATCGCG 600 CAAATTAAGG GCAAAATTTC TTCGTATGTT TATACCACCA ATAACGGTAG CTTGAGTTTA 660 AGGCCTTTTT ATGAATCGTT TTTGTTAGAA AAAAAGAGCG ATAATGTTTA TACGATAGAG 720 AATAAGGCTT TAGATACTAT GGAGATTTCA AAGTGTCAAA TGGTGTTAAA AAAGCATTCA 780 ACCGATAAAT TAGACAGCCA GCATAAAGCC ATCAGTATTG ATTTGGATTT TAAAAAAGAG 840 CGCTTTAAGA GCGATACGGA ACTCTTTTTA GAATGTCTTA AGGAAAGTTA G 891 (2) INFORMATION FOR SEQ ID NO:39: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 747 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double

(D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...747 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:39: GTGAGCTATG ACAACACCGA TGATTATTAT TTCCCTAGAA ATGGGGTTAT CTTTAGTTCC 60 TATGCGACAA TGTCTGGTTT GCCAAGCTCT GGCACGCTCA ATTCTTGGAA CGGGTTAGGC 120 GGGAATGTCC GTAACACCAA AGTTTATGGT AAATTCGCCG CTTACCACCA TTTGCAAAAA 180 TATTTATTGA TAGATTTGAT CGCTCGTTTT AAAACGCAAG GGGGCTATAT CTTTAGGTAT 240 AACACCGATG ATTACTTGCC CTTAAACTCC ACTTTCTACA TGGGGGGCGT AACCACGGTG 300 AGAGGCTTTA GGAACGGCTC AATCACACCT AAAGATGAGT TTGGCTTGTG GCTTGGAGGC 360 GATGGGATTT TTACCGCTTC TACTGAATTG AGCTATGGGG TGTTAAAAGC GGCTAAAATG 420 CGTTTAGCGT GGTTTTTTGA CTTTGGTTTC TTAACCTTTA AAACCCCAAC TAGGGGGAGT 480 TTCTTCTATA ACGCTCCCAC CACGACGGCG AATTTTAAAG ATTATGGCGT TGTAGGGGCT 540 GGGTTTGAAA GGGCGACTTG GAGGGCTTCT ACAGGCTTAC AGATTGAATG GATTTCGCCC 600 ATGGGGCCTT TGGTGTTGAT TTTCCCTATA GCGTTTTTCA ACCAATGGGG CGATGGCAAT 660 GGCAAAAAAT GTAAAGGGCT GTGCTTTAAC CCTAACATGA ACGATTACAC GCAACATTTT 720 GAATTTTCTA TGGGAACAAG GTTTTAA 747 (2) INFORMATION FOR SEQ ID NO:40: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1008 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...1008 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:40: GTGCAACACT TCAATTTCCT CTATAAAGAT TCTTTATTTT CTATCGCTTT ATTCACTTTC 60

ATTATCGCTC TTGTGATTTT ATTAGAACAG GCTAGAGCGT ATTTCACCCG AAAGAGAAAC 120 AAAAAATTTT TGCAAAAATT CGCCCAAAAT CAAAACGCCT ATGCGAGCAG CGAGAATTTA 180 GACGAGCTTT TAAAGCATGC TAAAATTTCC AGTTTGATGT TTTTAGCTAG GGCGTATTCT 240 AAAGCGGATG TGGAAATGAG CATTGAAATC TTAAAAGGGC TTTTGAATCG CCCCTTAAAA 300 GATGAAGAAA AAATCGCTGT TTTAGATTTA TTGGCTAAAA ATTATTTTAG CGTGGGGTAT 360 TTGCAGAAAA CAAAAGACAC CGTGAAAGAA ATTTTGCGCT TTTCCCCAAG GAATGTGGAA 420 GCGTTGTTGA AGCTTTTGCA TGCGTATGAA TTAGAAAAAG ATTATTCAAA GGCTTTAGAA 480 ACTTTGGAAT GTTTGGAAGA ATTAGAGGTG CCTAAAATTG AAACGATTAA AAATTACCTC 540 TATTTAATGC ATTTAATAGA GAATAAGGAA GATGCGGCTA AAATCTTGCA TGTTTCAAAA 600 GCGTCGTTAG ATTTGAAAAA AATCGCTCTG AATCACTTAA AATCGCATGA TGAAAATCTT 660 TTTTGGCAAG AAATTGATAC AACCGAACGG CTAGAAAATG TGATCGATCT TTTATGGGAT 720 ATGAATATCC CTGCTTTTAT TTTAGAAAAA CATGCCCTTT TGCAGGACAT CGCGCGATCT 780 CAAGGGTTGC TTTTGGATCA CAAACCTTGC CAAATTTTTG AATTAGAGGT TTTACGCGCT 840 CTATTGCATA GCCCTATAAA AGCGAGTCTG ACTTTTGAAT ACCGCTGCAA GCATTGCAAA 900 CAAATCTTTC CTTTTGAAAG CCATAGGTGT CCTGTGTGTT ACCAGTTAGC GTTTATGGAT 960 ATGGTGCTTA AAATCTCTAA AAAAACGCAT GCTATGGGAG TGGATTAA 1008 (2) INFORMATION FOR SEQ ID NO:41: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1242 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...1242 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:41: ATGAGGAAAA TTTTTTCTTA TATTTCTAAG GTTCTATTAT TTATTGGGGT GGTTTATGCA 60 GAGCCTGATT CTAAAGTGGA AGCCTTAGAA GGGAGGAAGC AAGAGTCTTC TTTGGATAAA 120 AAAATCCGCC AAGAATTGAA GAGTAAGGAA TTGAAGAATA AGGAATTAAA GAATAAGGAT 180 TTGAAAAATA AAGAAGAAAA GAAAGAAACA AAAGC CAAGA GAAAACCCAG AGCAGAAGTC 240 CATCATGGGG ACGCCAAAAA TCCCACTCCA AAGATCACGC CTCCTAAAAT CAAAGGGAGT 300 AGTAAGGGCG TTCAAAATCA AGGCGTTCAA AACAACGCGC CAAAACCTGA AGAAAAAGAT 360 ACAACCCCTC AAGCTACTGA AAAAAATAAG GAAACAAGCC CTAGCTCTCA ATTCAATTCC 420 ATTTTTGGTA ATCCTAATAA CGCTACCAAC AACACCCTTG AAGATAAGGT CGTAGGGGGC 480 ATTTCATTGC TTGTTAATGG TTCGCCTATC ACGCTGTATC AAATCCAAGA AGAGCAAGAA 540 AAATCTAAAG TGAGTAAGGC TCAAGCTAGG GATCGTTTGA TCGCTGAACG CATTAAAAAC 600 CAAGAAATTG AGCGCTTAAA AATCCATGTA GATGATGACA AGCTAGACCA AGAAATGGCG 660 ATGATGGCGC AACAACAAGG CATGGATTTA GACCATTTCA AACAGATGCT TATGGCTGAG 720 GGGCATTATA AACTCTATAG AGATCAACTT AAAGAGCATT TAGAAATGCA AGAATTGTTG 780 CGTAATATTT TGCTCACGAA TGTGGATACC AGCTCTGAAA CCAAAATGCG CGAATATTAC 840 AACAAACACA AGGAGCAATT CAGTATCCCC ACAGAAATAG AAACCGTGCG CTACACTTCC 900

ACCAATCAAG AGGATTTAGA AAGGGCTATG GCAGACCCTA ATTTGGAAGT CCCAGGGGTG 960 AGTAAGGCCA ATGAAAAAAT AGAGATGAAA ACCCTAAACC CTCAAATCGC CCAAGTCTTT 1020 ATTTCGCATG AGCAAGGCTC TTTCACGCCC GTTATGAATG GGGGTGGGGG GCAGTTCATC 1080 ACCTTTTATA TCAAGGAAAA AAGGGGTAAA AATGAAGTGA GCTTCAGTCA GGCCAAGCAA 1140 TTCATCGCCC AAAAATTAGT GGAAGAATCT AAGGATAAGA TTTTAGAAGA GCATTTTGAA 1200 AAATTGCGCG TTAAGTCTAG GATTGTGATG ATCAGAGAGT GA 1242 (2) INFORMATION FOR SEQ ID NO:42: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 561 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...561 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:42: ATGATTAAAA GAATTGCTTG TATTTTAAGC TTGAGCGCGA GTTTAGCGTT AGCTGGCGAA 60 GTGAATGGGT TTTTCATGGG TGCGGGTTAT CAACAAGGTC GTTATGGCCC TTATAACAGC 120 AATTACTCTG ATTGGCGTCA TGGCAATGAC CTTTATGGTT TGAATTTCAA ATTAGGTTTT 180 GTAGGCTTTG CCAATAAATG GTTTGGGGCT AGGGTGTATG GCTTTTTAGA TTGGTTTAAC 240 ACTTCAGGGA CTGAACACAC CAAAACCAAT TTGCTCACCT ATGGCGGCGG TGGCGATTTG 300 ATTGTCAATC TCATTCCTTT GGATAAATTC GCTCTAGGTC TCATTGGTGG CGTTCAATTA 360 GCCGGAAACA CTTGGATGTT CCCTTATGAT GTCAATCAAA CCAGATTCCA GTTCTTATGG 420 AATTTAGGCG GAAGAATGCG TGTTGGGGAT CGCAGTGCGT TTGAAGCGGG CGTGAAATTC 480 CCTATGGTTA ATCAGGGTAG CAAAGATGTA GGGCTTATCC GCTACTATTC TTGGTATGTG 540 GATTATGTCT TCACTTTCTA G 561 (2) INFORMATION FOR SEQ ID NO:43: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 729 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO

(vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...729 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:43: ATGAAAAAAT TTTTTTCTCA ATCTTTGTTA GCTCTTATTA TCTCTATGAA TGCGGTATCT 60 GGCATGGATG GTAATGGCGT TTTTTTAGGG GCGGGTTATT TGCAAGGACA GGCGCAAATG 120 CATGCGGATA TTAATTCTCA AAAACAAGCC ACCAACGCTA CGATCAAAGG CTTTGACGCG 180 CTCTTGGGGT ATCAATTTTT CTTTGAAAAA CACTTTGGCT TACGCCTTTA TGGGTTTTTT 240 GACTACGCTC ATGCCAATTC TATTAAGCTT AAAAACCCTA ACTATAATAG CGAAGCGGCG 300 CAAGTGGCTA GTCAAATTCT TGGGAAACAA GAAATCAATC GTTTAACAAA CATTGCCGAT 360 CCCAGAACTT TTGAGCCGAA CATGCTCACT TATGGGGGGG CTATGGACGT GATGGTTAAT 420 GTCATCAATA ACGGCATCAT GAGTTTGGGG GCTTTTGGCG GGATACAATT GGCCGGCAAT 480 TCATGGCTTA TGGCGACACC GAGCTTTGAG GGCATTTTAG TGGAACAAGC CCTTGTGAGC 540 AAGAAAGCCA CTTCTTTCCA ATTTTTATTC AATGTGGGGG CTCGCTTAAG GATCTTAAAA 600 CATTCTAGCA TTGAAGCGGG CGTGAAATTC CCCATGCTAA AGAAAAACCC CTACATCACT 660 GCAAAAAATT TGGATATAGG GTTTAGGCGC GTGTATTCGT GGTATGTGAA TTACGTGTTC 720 ACTTTCTAG 729 (2) INFORMATION FOR SEQ ID NO:44: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 771 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1. .771 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:44: ATGGGATACG CAAGCAAATT AGCTTTAAAG ATTTGTTTGG TAGGTTTATG TTTATTTAGC 60 ACCCTTGGTG CAGAACACCT TGAGCAAAAA GGGAATTATA TTTATAAGGG AGAGGAGGCT 120 TATAATAATA AGGAATATGA GCGAGCGGCT TCTTTTTATA AGAGCGCTAT TAAAAATGGT 180 GAGTCGCTTG CTTATATTCT TTTAGGGATC ATGTATGAAA ATGGTAGGGG TGTACCTAAA 240 GATTACAAGA AAGCGGTTGA ATATTTCCAA AAAGCTGTTG ATAACGATAT ACCTAGAGGG 300 TATAACAATT TGGGCGTGAT GTATAAAGAG GGTAAGGGAG TTCCTAAAGA TGAAAAGAAA 360 GCGGTGGAAT ATTTTAGAAT AGCTACAGAG AAAGGTTATA CTAACGCTTA TATCAACTTA 420 GGCATCATGT ATATGGAGGG CAGGGGAGTT CCAAGTAACT ATGCGAAAGC GACAGAATGT 480 TTTAGAAAAG CGATGCATAA GGGCAATGTG GAAGCTTATA TTCTCCTAGG GGATATTTAT 540

TATAGCGGGA ATGATCAATT GGGTATTGAG CCGGACAAAG ATAAGGCTGT TGTCTATTAT 600 AAAATGGCGG CTGATGTGAG TTCTTCTAGA GCTTATGAAG GGTTGTCAGA GTCTTATCGG 660 TATGGGTTAG GCGTGGAAAA AGATAAAAAA AAGGCTGAAG AATACATGCA AAAAGCATGC 720 GATTTTGACA TTGATAAAAA TTGTAAGAAA AAGAACACTT CAAGCCGATA A 771 (2) INFORMATION FOR SEQ ID NO:45: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1974 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...1974 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:45: ATGAGAAAAC TATTCATCCC ACTTTTATTA TTCAGCGCTT TAGAAGCGAA CGAGAAAAAC 60 GGCTTTTTCA TAGAAGCCGG CTTTGAAACT GGGCTATTAG AAGGCACACA AACGCAAGAA 120 AAAAGACACA CCACCACAAA AAACACTTAC GCAACTTACA ATTATTTACC CACAGACACG 180 ATTTTAAAAA GAGCGGCTAA TTTATTCACC AATGCCGAAG CGATTTCAAA ATTAAAATTC 240 TCATCTTTAT CCCCTGTTAG AGTGTTGTAT ATGTATAATG GTCAATTAAC TATAGAAAAC 300 TTCTTGCCTT ATAATTTAAA TAATGTTAAG CTTAGTTTTA CAGACGCTCA AGGCAATGTG 360 ATCGATCTAG GCGTGATAGA GACTATCCCC AAACACTCTA AGATTGTTTT GCCCGGAGAG 420 GCATTTGATA GTCTAAAAAT TGACCCCTAT ACTTTATTTC TTCCAAAAAT TGAAGCCACT 480 AGCACTTCTA TTTCTGACGC TAACACGCAG AGGGTGTTTG AAACGCTCAA TAAGATTAAG 540 ACAAATTTGG TCGTAAATTA TAGGAATGAA AACAAATTTA AAGATCACGA AAATCATTGG 600 GAAGCCTTTA CCCCACAAAC CGCAGAAGAA TTCACTAATT TAATGTTGAA CATGATCGCT 660 GTTTTAGACT CCCAATCTTG GGGCGATGCG ATCTTAAACG CTCCTTTTGA GTTCACTAAC 720 AGCCCAACAG ATTGCGATAA TGATCCTTCA AAATGCGTAA ATCCTGGGAC AAACGGGCTT 780 GTCAATTCTA AAGTCGATCA AAAATATGTG TTAAACAAAC AAGACATTGT CAATAAATTT 840 AAAAACAAAG CGGATCTTGA TGTAATTGTT TTAAAGGATT CAGGGGTTGT AGGGCTTGGG 900 AGTGATATTA CCCCTAGCAA CAATGATGAT GGCAAGCATT ATGGCCAGTT AGGGGTAGTA 960 GCTTCTGCTT TAGATCCTAA AAAACTCTTT GGCGATAACC TTAAGACTAT CAATTTAGAG 1020 GATTTAAGAA CCATCTTGCA TGAATTCAGC CACACTAAAG GCTATGGGCA TAACGGGAAT 1080 ATGACCTATC AAAGAGTGCC GGTAACGAAA GATGGTCAAG TGGAAAAGGA TAGTAATGGC 1140 AAGCCAAAAG ATTCTGATGG CCTCCCCTAT AATGTGTGTT CGCTTTATGG GGGATCCAAT 1200 CAGCCCGCTT TCCCTAGCAA CTACCCTAAT TCCATCTATC ACAATTGTGC GGATGTCCCG 1260 GCTGGCTTTT TAGGGGTAAC AGCAGCGGTT TGGCAGCAGC TCATCAATCA AAACGCCTTG 1320 CCGATCAACT ACGCTAACTT GGGGAGTCAA ACAAACTACA ACCTAAACGC TAGTTTAAAC 1380 ACGCAAGATT TAGCCAATTC CATGCTCAGC ACCATCCAAA AAACCTTTGT AACTTCTAGC 1440 GTTACCAACC ACCATTTTTC AAACGCATCG CAAAGTTTTA GAAGCCCTAT TTTAGGGGTT 1500 AACGCTAAAA TAGGCTATCA AAACTACTTT AATGATTTCA TAGGGTTGGC TTATTATGGC 1560 ATCATCAAAT ACAATTACGC TAAAGCTGTT AATCAAAAAG TCCAGCAATT GAGCTATGGT 1620

GGGGGGATAG ATTTGTTATT GGATTTCATC ACCACTTACT CCAATAAAAA TAGCCCTACA 1680 GGCATTCAAA CCAAAAGGAA TTTTTCTTCA TCTTTTGGTA TCTTTGGGGG GTTAAGGGGC 1740 TTGTATAACA GCTATTATGT GTTGAACAAA GTCAAAGGAA GCGGCAATTT AGATGTGGCT 1800 ACCGGGTTGA ACTACCGCTA TAAGCATTCT AAATATTCTG TAGGGATTAG CATCCCTTTA 1860 ATCCAAAGAA AAGCTAGCGT CGTTTCTAGC GGTGGCGATT ATACGAACTC TTTTGTTTTC 1920 AATGAAGGGG CTAGCCACTT TAAGGTGTTT TTCAATTACG GGTGGGTGTT TTAG 1974 (2) INFORMATION FOR SEQ ID NO:46: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 504 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...504 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:46: ATGAAATTGG TGAGTCTTAT TGTAGCGTTA GTTTTTTGTT GTTTTTTAGG GGCTGTAGAG 60 TTGCCTGGAG TTTATCAAAC TCAAGAATTT TTATACATGA AAAGCTCTTT TGTGGAGTTT 120 TTTGAGCATA ACGGGAAGTT CTATGCCTAT GGTATTTCTG ATGTGGATGG CTCTAAAGCC 180 AAAAAAGACA AACTCAATCC TAACCCAAAG CTAAGGAATC GCAGCGATAA AGGCGTGGTG 240 TTTTTAAGCG ATTTGATTAA GGTTGGGGAA CAATCTTATA AAGGCGGTAA GGCGTATAAT 300 TTTTATGACG GCAAGACCTA CCATGTGAGA GTCACTCAAA ATTCAAACGG GGATTTGGAA 360 TTCACTTCAA GCTATGACAA ATGGGGGTAT GTGGGCAAAA CCTTCACCTG GAAACGCCTG 420 AGCGATGAAG AAATCAAAAA TCTAAAGCTC AAGCGTTTTA ACTTGGACGA AGTCCTTAAA 480 ACCCTCAAAG ATAGCCCTAT TTAA 504 (2) INFORMATION FOR SEQ ID NO:47: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 885 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE:

(A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...885 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:47: ATGAGTAATC AAGCGAGCCA TTTGGATAAT TTTATGAACG CTAAAAATCC CAAAAGTTTT 60 TTTGATAATA AGGGGAATAC CAAATTCATC GCTATCACAA GCGGTAAGGG GGGCGTGGGG 120 AAATCCAACA TTAGCGCTAA TTTAGCTTAC TCTTTATACA AGAAAGGTTA TAAGGTAGGG 180 GTATTTGATG CGGATATTGG TTTAGCGAAT TTAGATGTCA TTTTTGGGGT GAAAACCCAT 240 AAAAATATCT TGCATGCCTT AAAAGGCGAA GCCAAATTGC AAGAAATCAT TTGCGAGATT 300 GAACCCGGGC TTTGCTTAAT CCCTGGGGAT AGCGGCGAAG AAATTTTAAA ATACATCAGC 360 GGCGCGGAAG CTTTGGATCG ATTCGTAGAT GAAGAGGGGG TTTTAAGCTC TTTAGATTAT 420 ATTGTGATTG ATACGGGTGC TGGGATTGGG GCCACTACGC AAGCGTTTTT GAATGCGAGC 480 GATTGCGTGG TGATTGTTAC CACACCCGAT CCTTCAGCGA TTACCGATGC GTATGCATGC 540 ATTAAAATCA ACTCCAAGAA TAAAGATGAA TTGTTCCTTA TCGCTAACAT GGTAGCCCAA 600 CCTAAAGAAG GCAGGGCGAC TTATGAAAGG CTATTCAAGG TGGCTAAAAA CAATATCGCT 660 TCATTAGAAT TGCACTATTT AGGGGCGATT GAAAACAGCT CCTTATTGAA ACGCTATGTG 720 AGGGAGCGAA AGATTTTGAG GAAAATAGCC CCTAACGATT TGTTTTCGCA ATCCATTGAC 780 CAGATAGCGA GCCTTTTAGT TTCTAAACTA GAAACCGGCA CTTTAGAAAT ACCAAAAGAA 840 GGTTTAAAAA GCTTTTTTAA AAGGCTTTTG AAGTATTTGG GGTAG 885 (2) INFORMATION FOR SEQ ID NO:48: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1119 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...1119 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:48: TTGGAACCTT CAAGAAATCG CCTAAAACAT GCCGCCTTTT TTGTGGGGCT TTTTATCGTT 60 TTGTTTTTAA TTATAATGAA GCACCAAACC TCCCCCTATG CTTTCACGCA TAATCAAGCC 120 CTTGTCACTC AAACCCCCCC CTATTTCACG CAACTCACTA TCCCTAAACC AAATGACGCT 180 TTAAGCGCGC ATGCGAGCTC TTTAATCAGC TTGCCTAACG ACAATCTTTT GAGCGCTTAT 240 TTTAGCGGCA CTAAAGAAGG GGCAAGGGAT GTGAAAATCA GCGCGAATCT TTTTGACAGC 300 AAGACTAATC GCTGGAGCGA AGCCTTCATT CTTTTAACCA AAGAAGAGCT TTCTCATCAT 360 TCGCATGAAT ACATCAAAAA ATTAGGTAAC CCCTTGCTTT TTTTGCATGA TAATAAAATT 420 TTGTTGTTTG TCGTAGGGGT GAGCATGGGC GGGTGGGCCA CTTCTAAAAT CTATCAATTT 480

GAAAGCGCTT TAGAGCCGAT TCATTTTAAG TTTGCGCGAA AACTCTCTTT AAGCCCTTTT 540 TTAAATTTGA GCCATTTAGT AAGGAATAAG CCTTTAAACA CCACTGATGG CGGGTTTATG 600 CTACCACTCT ATCACGAATT AGCCACCCAA TACCCCTTGT TGTTGAAATT TGACCAACAA 660 AATAACCCAA GAGAGCTTTT AAGGCCTAAT ACCTTAAACC ACCAGCTCCA ACCAAGCTTA 720 ACCCCCTTTA AAGACTGCGC TGTCATGGCG TTTAGAAACC ATTCTTTTAA AGATAGCCTC 780 ATGCTAGAAA CCTGTAAAAC CCCCACTGAT TGGCAAAAAC CCATTTCTAC AAATCTTAAA 840 AACTTAGATG ATTCTTTAAA TTTACTCAAT TTAAATGGAA TATTGTATTT GATCCACAAC 900 CCTAGCGATT TATCACTGCG TCGTAAAGAA CTTTGGCTTT CTAAATTAGA AAACTCCAAC 960 TCGTTTAAAA CCTTAAAAGT TTTGGATAAA GCGAATGAAG TGAGTTACCC AAGCTATAGC 1020 CTTAATCCGC ATTTTATAGA TATTGTCTAT ACTTACAACC GCTCTCATAT CAAACACATC 1080 CGTTTCAATA TGGCTTATTT AAATTCCCTT CTCAAGTGA 1119 (2) INFORMATION FOR SEQ ID NO:49: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 2937 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...2937 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:49: ATGAAGAAAA GAAAACATGT ATCCAAGAAA GTGTTTAATG TCATTATCTT GTTTGTGGCA 60 GTATTCACTC TTTTAGTCGT CATTCACAAA ACCCTTTCAA ACGGCATTCA CATACAAAAT 120 TTAAAAATTG GAAAACTTGG CATTTCTGAA TTATACTTAA AACTCAATAA CAAGCTTTCT 180 TTGGAAGTTG AGCGGGTTGA TCTCTCTTCT TTCTTCCATC AAAAACCCAC TAAAAAGCGT 240 TTAGAAGTTT CTGATTTGAT TAAAAATATC CGTTATGGCA TTTGGGCGGT GTCTTATTTT 300 GAAAAACTTA AAGTCAAAGA AATCATTTTA GACGATAAAA ATAAAGCCAA TATCTTTTTT 360 GATGGGAATA AATACGAGTT AGAATTTCCA GGAATCAAAG GGGAATTTTC CCTAGAAGAC 420 GATAAAAATA TCAAGCTTAA AATCATCAAT TTGCTTTTTA AAGATGTTAA AGTCCAAGTG 480 GATGGCAACG CCCACTATTC ACCCAAAGCC AGGAAAATGG CGTTCAATTT GATTGTCAAG 540 CCCTTAGTTG AACCCAGCGC TGCAATTTAT TTGCAAGGGC TAACCGATTT AAAAACCATA 600 GAATTAAAAA TTAACACTTC TCCAATGAAA AGCCTAGCGT TTTTAAAGCC TCTTTTCCAA 660 CGCCAATCGC AAAAAAATTT AAAAACGTGG ATTTTTGACA AGATCCAATT TGCCAGCTTT 720 AAGATTGATA ACGCTTTAAT CAAGGCTAAT TTCACTCCTA GCGAGTTTAT CCCATCGCTT 780 TTGGAAAATT CTGTAGTTAA AGCCACTTTG ATTAAGCCTT CAGTCGTTTT TAATGATGGC 840 TTATCGCCCA TTAAAATGGA TAAAACCGAA TTGATTTTCA AAAACAAACA GCTCCTCATA 900 CAGCCCCAAA AAATCACTTA TGAAACCATG GAATTAACCG GCTCTTACGC CACTTTTTCC 960 AATTTGTTAG AAGCCCCTAA GTTGGAGGTT TTTTTAAAAA CGACCCCTAA TTATTATGGC 1020 GATAGCATTA AGGATTTATT GAGCGCTTAT AAAGTCGTTT TACCTTTGGA TAAAATCAGC 1080 ATGCCATCTA GCGCGGATTT GAAGCTCACT TTGCAATTCT TAAAAAACAC CGCCCCCTTA 1140 TTTAGCGTTC AAGGCAGCGT TAATTTGCAA GAAGGCACTT TCTCGCTCTA TAATATCCCC 1200

CTTTACACGC AAAGCGCTCA AATCAATTTG GACATCGCCC AAGAATACCA ATACATCTAC 1260 ATAGACACGA TCCACACGCG CTATGCAAAC ATGCTGGATT TAGACGCTAA AATCGCTTTA 1320 GATTTAGGTC AAAAAAACCT TTCTTTGGAT TCTTTAGTCC ATAAAATCCA AGTCAATACC 1380 AATAACAATA TCAACATGCG CTCTTATGAT CCCAATAACA CTCAAGAAGA TCCGCAAACT 1440 AACTTTACTT TGGATCTAAA AAGCTTGCAT TCTATCATTC AAGAGGGTGA AAATTCAGAA 1500 GTTTTTAGAA GAAAAATCAT AGACACCATT AAAGCCCAAA GCGAAGATAA ATTCACTAAA 1560 GATGTTTTTT ACGCCACAGG AGACACTCTC AAAAGCCTGT CGTTGAGTTT TGATTTTTCC 1620 AACCCCGATC ACATACAATG GAGCGTGCCA CAACTCTTAT TAGAAGGCGA ATTTAAAGAT 1680 AACGCCTATA CTTTTAAGAT CAAAGATTTG AAAAAGATCA AGCCCTATTC CCCCATTATG 1740 GACTATATTG CCCTAAAAGA CGGCTCTTTA GAGGTTTCTA CGAGCGATTT TGTCAATATT 1800 GATTTTTTTG CTAAAGATTT GAAAATCAAC CTCCCCATTT ATAGGAGCGA TGGATCGCAT 1860 TTTGATTCTT TTTCTTTATT TGGCTCTATC AATAAAGATG AAATTTCTGT CTATACTCCA 1920 AGCAAAAGCA TATCCATAAA AGTTAAGGGG GATCAAAAGG ATATTACCCT TAATAACATT 1980 GATTTGAGTA TTGATGATTT CTTGGATAGT AAAATGCCAG CTATTGCGGG ATTATTCTCA 2040 AAAGAACGAA AAGAAAAGCC TAGCTCTAAA GAAATCCAAG ATGAAGATGT TTTCATTAGC 2100 GCCAAACAAC GCTATGAAAA AGCCCACAAA ATTATCCCCA TCTCTACACG CATCCATGCT 2160 AAAGATGTCG TGCTGATCTA TAAAAAAATG CCTTTTCCTT TAGAAAATCT TGATATTGTC 2220 GCTCAAGACG ATAGGGTGAA AATTGATGGC AATTATAAAA ACGCCATGAT CATGGCGGAT 2280 TTAGTGCATG GGGCTTTGTA TCTTAAGGCT CATAATTTTA GCGGGGATTA TATCAACACC 2340 ATTCTTCAAA AAGATTTCGT AGAAGGAGGC TTATTCACGC TTATTGGGGC TCTTGAAGAT 2400 CAGGTTTTCA ATGGCGAATT GAAATTCCAA AACACAAGCT TAAAGAATTT CGCCCTCATG 2460 CAAAACATGG TCAATCTCAT CAACACCATT CCCTCCCTCA TTGTCTTTAG AAACCCTCAT 2520 TTAGGGGCTA ATGGCTATCA AATCAAAACC GGCTCCGTTG TGTTTGGGAT CACTAAAGAA 2580 TATTTAGGGT TAGAAAAAAT TGATCTTGTC GGCAAAACGC TTGATATTGC TGGCAATGGA 2640 ATCATTGAAT TAGACAAAAA CAAATTAGAT TTAAACTTAG AAGTTTCCAC TATCAAGGCT 2700 TTGAGTAATG TCTTAAATAA AATCCCTATC GTGGGCTATC TCGTTTTAGG AAAAGGAGGT 2760 AAAATCACCA CTAACGTGAA TGTCAAAGGC ACGTTGGATA AGCCTAAAAC CCAAGTAACT 2820 TTAGCGTCAG ATATTATCCA AGCGCCTTTT AAAATCTTAC GCCGTATTTT CACGCCTATT 2880 GACATCATCG TGGATGAAGT CAAGAAAAAC ATTGATTCAA AAAGGAAATT AAAATGA 2937 (2) INFORMATION FOR SEQ ID NO:50: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1434 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...1434 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:50: ATGAATACTA TTATAAGATA TGCGAGTTTA TGGGGCTTGT GTATTACTCT AACTCTAGCG 60 CAAACCCCCT CTAAAACCCC TGATGAAATC AAGCAAATCC TTAACAATTA TAGCCATAAG 120

AATTTAAAGC TCATTGATCC GCCGACAAGT TCTTTAGAAG CGACACCGGG TTTTTTACCC 180 TCGCCTAAAG AAACAGCGAC CACGATCAAT CAAGAGATCG CTAAATACCA TGAAAAAAGC 240 GATAAAGCCG CTTTGGGGCT TTATGAATTG CTAAAGGGGG CTACCACCAA TCTCAGTTTG 300 CAAGCGCAAG AACTCAGTGT CAAGCAAGCG ATGAAGAACC ACACCATCGC CAAAGCGATG 360 TTTTTGCCTA CTTTGAACGC GAGTTATAAT TTTAAAAATG AAGCTAGGGA TACTCCAGAA 420 TATAAGCATT ATAACACCCA ACAACTCCAA GCTCAAGTCA CATTGAATGT GTTTAATGGC 480 TTTAGCAATG TGAATAATGT CAAAGAAAAG TCTGCGACTT ACCGATCCAC TGTGGCTAAT 540 TTAGAATATA GCCGCCAAAG CGTGTATTTG CAAGTGGTGC AACAATACTA CGAGTATTTT 600 AACAATCTCG CTCGCATGAT CGCTTTGCAA AAGAAATTAG AGCAAATCCA AACGGACATT 660 AAAAGGGTTA CTAAGCTCTA TGACAAAGGG CTGACCACGA TTGATGATTT ACAAAGCTTA 720 AAAGCGCAAG GGAATTTGAG CGAATACGAT ATTTTGGACA TGCAATTTGC TTTGGAGCAA 780 AACCGCTTGA CTTTAGAATA CCTCACTAAC CTCAGTGTGA AAAATTTGAA AAAGACCACG 840 ATTGATGCGC CTAATTTGCA ATTAAGAGAA AGGCAGGATT TGGTTTCTTT AAGGGAGCAG 900 ATTTCTGCAC TCAGATACCA AAACAAGCAA CTCAATTATT ACCCCAAGAT AGATGTGTTT 960 GACTCATGGC TTTTTTGGAT CCAAAAACCC GCTTATGCCA CAGGGCGTTT TGGGAATTTC 1020 TACCCAGGTC AGCAAAATAC GGCTGGGGTT ACTGCGACTT TGAATATTTT TGATGATATA 1080 GGGTTGAGCT TGCAAAAACA ATCCATCATG CTAGGCCAAT TAGCGAATGA AAAGAATTTA 1140 GCGTATAAAA AATTGGAGCA AGAAAAAGAC GAACAGCTTT ACAGAAAGTC GCTTGATATT 1200 GCCAGAGCTA AGATTGAATC TTCAAAGGCT AGTTTGGATG CGGCCAATCT TTCTTTTGCC 1260 AATATTAAAA GGAAATACGA CGCTAATTTA GTGGATTTCA CTACCTATTT AAGGGGCTTA 1320 ACCACGCGCT TTGATGCAGA AGTGGCTTAC AATTTAGCGC TCAACAATTA CGAAGTGCAA 1380 AAAGCCAATT ACATTTTTAA CAGCGGGCAT AAAATAGACG ACTATGTGCA TTAA 1434 (2) INFORMATION FOR SEQ ID NO:51: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1239 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...1239 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:51: ATGCTATCTT TTATAAGCGC GTTTGATAAA AGGGGCGTTT CAATACGCCT TCTAACAGCC 60 TTGTTACTGC TTTTTAGTTT GGGTTTGGCT AAAGATTTAG AAATCCAAAC TTTTGTGGCT 120 AAATACCTTT CTAAAAATCA AAAAATACAA GCCCTACAGG AGCAAATTGA CGCTTTAGAT 180 TCTCAAGAAA AAGTCGTTAG CAAATGGGAT AACCCTATTT TGTATTTAGG CTATAACAAC 240 GCTAACGTGA GCGATTTTTT CAGGCTGGAT AGCACCTTAA TGCAAAACAT GAGCTTGGGT 300 TTGTCTCAAA AAGTGGATTT AAATGGTAAA AAACTCACGC AGTCTAAAAT GATCAATTTA 360 GAAAAACAAA AAAAAATATT AGAGCTTAAA AAAACCAAGC AGCAATTGGT GATTAATTTA 420 ATGATAAACG GCATTGAAAA CTATAAAAAC CAACAAGAAA TAGAGCTTTT AAACACAGCG 480 ATTAAAAATT TAGAAAACAC CCTCTATCAA GCCAACCATT CCAGTTCGCC CGATTTAATA 540

GCGATCGCCA AGTTAGAAAT TTTAAAATCG CTATTAGAAA TCCAAAAAAA CGATTTAGAA 600 GTAGCGCTCT CTAGCAGCCA TTATTCCATG GGCGAATTGA CTTTTAAAGA AAACGAGATT 660 TTAAGCATTG CCCCTAAAAA TTTTGAATTC AATAACGAGC AAGAGCTGCA TAACATTAGC 720 GCCACTAATT ACGATATTGC GATCGCCAGG CTTGATGAAG AAAAAGCACA AAAAGACATC 780 ACTCTGGCTA AAAAAAGCTT TTTAGAAGAC ATAAACGTTA CCGGGGTGTA TTATTTCCGC 840 TCCAAACAAT ACTATAACTA CGACATGTTT AGCGTCGCTT TGTCTATCCC TTTACCTCTT 900 TATGGCAAGC AGGCTAAATT AGTGGAGCAA AAGAAAAAAG AAAGCTTGGC GTTTAAAAGC 960 GAAGTGGAAA ACGCCAAAAA CAAAACGCGC CACCTGGCCC TAAAACTCCT TAAAAAATTA 1020 GAAACCTTGC AAAAAAACCT GGAATCGATC AATAAAATCA TCAAACAGAA TGAAAAAATC 1080 GCGCAAATTT ATGCGCTTGA TTTGAAAACT AATGGCGATT ACAACGCTTA TTACAACGCC 1140 TTGAATGACA AAATCACTAT TCAAATCACC CAGCTTGAAA CCTTAAGCGC TCTAAATAGT 1200 GCTTATTTGT CCTTACAAAA TCTCAAAGGA TTAGAATGA 1239 (2) INFORMATION FOR SEQ ID NO:52: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 414 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...414 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:52: ATGCGTATAG TTAGAAATTT ATTTCTTGTA TCGTTTGTGG CGTATAGTAG TGCGTTCGCA 60 GCGGATTTAG AAACCGGAAC CAAAAACGAC AAAAAGAGCG GTAAAAAATT TTACAAACTC 120 CATAAAAACC ATGGCTCAGA AACCGAGACT AAAAACGATA AAAAGCTTTA TGATTTCACT 180 AAAAATAGCG GATTAGAAGG CGTGGATTTA GAAAAAAGCC CTAACCTTAA AAGCCATAAA 240 AAAAGCGATA AAAAGTTTTA TAAACAACTC GCTAAAAACA ATATCGCTGA AGGGGTGAGC 300 ATGCCGATTG TGAATTTCAA TAAAGCCCTA TCTTTTGGGC CTTATTTTGA AAGGACTAAA 360 AGCAAAAAAA CCCAATACAT GGACGGCGGG TTGATGATGC ACATCCGTTT TTAA 414 (2) INFORMATION FOR SEQ ID NO:53: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 930 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO

(iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...930 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:53: TTGATGCCAC AAAACCAGCT TGTGATCACC ATCATTGATG AATCAGGCTC TAAGCAACTC 60 AAATTTTCTA AAAATTTAAA ACGCAACCTC ATCATTTCTG TTGTCATTCT TTTATTGATC 120 GTGGGGCTTG GCGTGGGGTT TTTAAAATTT TTAATCGCTA AAATGGATAC GATGACAAGC 180 GAGAGGAATG CGGTTTTAAG GGATTTTAGG GGTTTGTATC AAAAAAATTA CGCCCTAGCG 240 AAAGAGATTA AAAACAAGCG AGAAGAGCTT TTTATTGTGG GGCAAAAGAT CCGTGGGCTA 300 GAATCCTTGA TTGAAATCAA AAAGGGGGCT AATGGGGGAG GGCATCTCTA TGATGAAGTG 360 GATTTAGAAA ATTTGAGCTT AAATCAAAAA CATTTAGCAC TCATGCTCAT TCCTAATGGC 420 ATGCCCCTAA AAACTTATAG CGCTATCAAA CCCACTAAAG AAAGGAACCA CCCCATTAAA 480 AAGATTAAGG GCGTTGAATC CGGGATCGAT TTTATCGCGC CATTGAACAC GCCTGTGTAT 540 GCGAGCGCTG ATGGGATTGT GGATTTTGTG AAGACTCGTT CTAATGCGGG GTATGGGAAC 600 TTGGTGCGCA TTGAACATGC GTTTGGTTTC AGCTCCATTT ATACGCACTT AGATCATGTC 660 AATGTGCAGC CTAAAAGCTT CATCCAAAAA GGGCAGTTGA TTGGCTATAG CGGGAAGAGC 720 GGTAATAGCG GCGGCGAAAA ATTGCATTAT GAAGTGCGGT TTTTGGGTAA AATTTTAGAC 780 GCAGAAAAAT TCCTAGCATG GGATTTGGAT CATTTTCAAA GCGCTTTAGA AGAAAATAAA 840 TTTATTGAAT GGAAGAATCT GTTTTGGGTT TTAGAAGACA TCGTCCAGCT CCAAGAGCAT 900 GTGGATAAAG ACACCTTAAA AGGTCAGTAG 930 (2) INFORMATION FOR SEQ ID NO:54: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 999 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...999 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:54: GTGCTATATT TTTTAACCAG TTTATTTATT TGCTCTTTGA TTGTTTTGTG GTCTAAAAAA 60 TCCATGCTCT TTGTGGATAA CGCTAATAAA ATCCAAGGCT TCCATCATGC AAGAACCCCA 120 CGAGCCGGGG GGCTTGGGAT CTTTCTTTCT TTTGCGTTGG CTTGTTATCT TGAACCTTTT 180

GAGATGCCTT TTAAGGGGCC TTTTGTTTTC TTAGGGCTAT CGCTAGTGTT TTTGAGCGGT 240 TTTTTAGAAG ACATTAACCT TTCATTAAGC CCCAAAATAC GCCTTATTTT GCAAGCTGTA 300 GGGGTCGTTT GCATCATTTC ATCAACGCCT TTAGTGGTGA GCGATTTTTC GCCCCTTTTT 360 AGCTTGCCTT ATTTCATCGC TTTTTTATTC GCTATTTTTA TGCTGGTGGG TATCAGTAAC 420 GCTATTAATA TCATTGACGG GTTTAACGGG CTTGCATCTG GGATTTGCGC GATCGCGCTT 480 TTAGTCATTC ATTATATAGA CCCTAGCAGT TTGTCTTGTT TGCTCGCTTA CATGGTGCTT 540 GGGTTTATGG TGTTAAATTT CCCTTCAGGA AAGATTTTTT TAGGCGATGG GGGGGCGTAT 600 TTTTTGGGTT TGGTGTGCGG GATTTCTCTC TTGCATTTGA GTTTGGAGCA AAAAATCAGC 660 GTGTTTTTTG GGCTCAATTT AATGCTTTAT CCGGTCATAG AGGTGCTTTT TAGTATCCTT 720 AGGCGCAAAA TAAAACGCCA GAAAGCCACC ATGCCGGATA ATTTGCATTT GCACACCCTT 780 TTATTTAAAT TCTTGCAACA ACGCTCTTTC AATTACCCTA ACCCTTTATG CGCGTTTATC 840 CTTATTCTAT GCAACCTGCC TTTTATTTTA ATAAGCGTTT TGTTTCGCTT GGACGCTTAT 900 GCGCTCATTG TGATTAGCCT AGTCTTTATC GCATGCTATT TAATAGGCTA TGCTTATTTG 960 AATAGGCAAG TTTGCGCTTT AGAAAAGCGG GCGTTTTAA 999 (2) INFORMATION FOR SEQ ID NO:55: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 816 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...816 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:55: ATGAACATAT TCAAGCGTAT TATTTGCGTA ACCGCTATTG TTTTAGGTTT TTTTAACCTT 60 TTAGACGCCA AACACCACAA AGAAAAAtA GAAGACCACA AAATCACTCG TGAGCTTAAA 120 GTGGGCGCTA ACCCTGTGCC GCATGCGCAA ATCTTGCAAT CAGTTGTGGA TGATTTGAAA 180 GAGAAAGGGA TCAAATTAGT GATCGTGTCT TTTACGGATT ATGTGTTGCC TAATTTAGCG 240 CTCAATGACG GCTCTTTAGA CGCGAATTAC TTCCAGCACC GCCCTTATTT GGATCGGTTT 300 AATTTGGACA GAAAAATGCA CCTTGTTGGT TTGGCCAATA TCCATGTGGA GCCTTTAAGA 360 TTTTATTCTC AAAAAATCAC AGACATTAAA AACCTTAAAA AAGGCTCAGT GATTGCTGTG 420 CCAAATGATC CGGCCAATCA AGGCAGGGCG TTGATTTTAC TCCATAAACA AGGCCTTATC 480 GCTCTCAAAG ACCCAAGCAA TCTATACGCT ACGGAGTTTG ATATTGTCAA AAATCCTTAC 540 AACATCAAAA TCAAACCCCT AGAAGCTGCG TTATTGCCTA AGGTTTTAGG GGATGTGGAT 600 GGGGCTATCA TAACAGGGAA TTATGCCTTG CAAGCAAAAC TCACCGGAGC CTTATTTTCA 660 GAAGATAAGG ACTCGCCTTA TGCTAATCTT GTAGCCTCTC GTGAGGATAA TGCGCAAGAT 720 GAAGCGATAA AAGCGTTGAT TGAAGCCTTA CAGAGCGAAA AGACCAGGAA ATTCATTTTG 780 GATACCTATA AGGGGGCGAT TATCCCGGCT TTTTAA 816 (2) INFORMATION FOR SEQ ID NO:56:

(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 951 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...951 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:56: ATGCAAGAAT TCAGTTTGTG GTGCGATTTT ATAGAAAGGG ATTTTTTAGA AAACGATTTT 60 TTAAAGCTCA TCAATAAGGG GGCTATTTGC GGGGCGACGA GTAACCCTAG TTTGTTTTGC 120 GAAGCGATCA CAAAAAGCGC GTTTTATCAA GATGAAATCG CTAAACTCAA AGGCAAAAAA 180 GCTAAAGAAA TTTATGAAAC TCTGGCACTA AAGGATATTT TACAAGCCTC TAGCGCGTTA 240 ATGCCTTTGT ATGAAAAAGA CCCTAACAAC GGCTACATCA GCCTAGAAAT TGACCCCTTT 300 TTAGAAGACG ATGCGATTAA AAGCATTGAT GAAGCCAAGC GGTTATTCAA AACATTAAAC 360 CGCCCCAATG TGATGATTAA AGTCCCGGCG AGTGAAAGCG CTTTTGAAGT CATTAGCGCT 420 CTGGCTCAAG CCTCTATCCC CATTAATGTA ACTTTAGTCT TTTCGCCTAA AATTGCCGGT 480 GAAATCGCTC AAATCTTAGC CAAAGAAGCA CGAAAAAGAG CGGTCATTAG CGTGTTTGTC 540 TCACGATTTG ACAAAGAAAT AGACCCACTA GTGCCACAAA ATTTGCAAGC TCAAAGTGGG 600 ATCATGAACG CTACCGAGTG TTATTATCAA ATCAACCAGC ATGCTAATAA GCTAATAAGC 660 ACCCTTTTTG CATCCACCGG CGTTAAATCT AATTCTTTAG CTAAAGATTA CTACATTAAA 720 GCGCTGTGTT TTAAAAACTC TATCAACACA GCCCCCCTAG ACGCCCTAAA CGCTTATTTG 780 CTTGACCCAA ACACCGAGTG TCAAACCCCT TTAAAAATCA CAGAAATTGA AGCGTTCAAA 840 AAAGAATTAA AAACGCACAA TATTGATTTA GAAAACACCG CCCAAAAACT CCTTAAAGAA 900 GGCTTGATAG CGTTCAAACA ATCCTTTGAA AAGCTTTTAA GCAGTTTTTG A 951 (2) INFORMATION FOR SEQ ID NO:57: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 783 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori

(ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...783 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:57: ATGAAAACAA ATGGTCATTT TAAGGATTTT GCATGGAAAA AATGCTTTTT AGGCGCGAGC 60 GTGGTGGCTT TATTAGTGGG GTGTAGCCCG CATATTATTG AAACCAATGA AGTTGCTTTG 120 AAATTGAATT ACCATCCAGC TAGCGAGAAA GTTCAAGCGT TAGATGAAAA GATTTTACTT 180 TTAAGGCCAG CTTTCCAATA CAGCGATAAT ATTGCTAAAG AGTATGAAAA CAAATTCAAG 240 AATCAAACCA CGCTTAAAGT TGAAGAGATC TTGCAAAATC AGGGCTATAA GGTTATTAAT 300 GTGGATAGCA GCGATAAAGA CGATTTTTCT TTTGCGCAAA AAAAAGAAGG GTATTTGGCT 360 GTCGCTATGA ATGGCGAAAT TGTTTTACGC CCCGATCCTA AAAGGACCAT ACAGAAAAAA 420 TCAGAACCCG GGTTATTATT CTCCACTGGT TTGGATAAAA TGGAAAGGGT TTTAATCCCG 480 GCTGGGTTTG TCAAGGTTAC CATACTAGAG CCTATGAGTG GGGAATCTTT GGATTCTTTT 540 ACGATGGATT TGAGCGAGTT GGACATCCAA GAAAAATTCT TAAAAACCAC CCATTCAAGC 600 CATAGCGGAG GGTTAGTTAG CACTATGGTT AAGGGGACGG ATAATTCTAA TGACGCAATT 660 AAGAGCGCTT TGAATAAGAT TTTTGCAAGT ATCATGCAAG AAATGGATAA GAAACTCACT 720 CAAAGGAATT TAGAATCTTA TCAAAAAGAC GCCAAGGAAT TAAAAAACAA GAGAAACCGA 780 TAA 783 (2) INFORMATION FOR SEQ ID NO:58: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4149 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...4149 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:58: TTGAATTTTA ATAACCTTAC GGCTAATGGG GCGTTAAATT TTAATGGTTA TGCGCCCTCT 60 TTAACTAAGG CTTTAATGAA TGTCAGCGGG CAGTTTGTTT TAGGGAATAA TGGGGATATT 120 AATTTATCTG ACATCAATAT CTTTGACAAC ATCACAAAAT CTGTAACTTA CAACATCTTA 180 AACGCTCAAA AAGGGATTAC TGGCATTAGT GGGGCTAATG GCTATGAAAA AATCCTTTTT 240 TATGGCATGA AAATCCAAAA CGCTACCTAT AGCGATAATA ACAACATCCA AACTTGGTCG 300 TTTATAAACC CTCTCAATTC TTCTCAAATC ATTCAAGAGA GCATTAAAAA TGGGGATCTA 360 ACCATAGAAG TTTTAAATAA CCCTAACTCG GCTTCCAACA CTATTTTTAA TATCGCTCCT 420 GAGCTTTATA ATTACCAAGA TTCTAAGCAA AATCCTACCG GCTATAGCTA TGATTATAGC 480 GACAATCAAG CAGGCACTTA TTACTTGACA AGCAACATTA AAGGTCTTTT CACCCCTAAA 540 GGCTCTCAAA CGCCTCAAAC CCCAGGCACT TATAGCCCAT TTAACCAGCC TTTGAATAGT 600 TTGAATATCT ACAATAAGGG TTTTTCTAGC GAGAATTTAA AAACGCTTTT AGGGATCCTT 660

TCTCAAAATT CCGCCACCTT AAAAGAAATG ATTGAATCCA ACCAACTAGA CAATATCACT 720 AACATTAATG AAGTGTTGCA ACTCTTAGAT AAGATTAAAA TCACCCAAGC GCAAAAGCAA 780 GCGCTCCTAG AAACGATCAA CCATTTGACT GACAACATCA ATCAAACCTT TAATAACGGG 840 AATCTCGTTA TAGGCGCTAC CCAAGATAAT GTTACAAACT CTACTAGCTC TATATGGTTT 900 GGGGGCAATG GCTATAGCAG CCCTTGCGCG CTAGATAGCG CCACTTGTTC TTCTTTTAGA 960 AACACTTACT TGGGGCAATT ATTAGGCTCA ACTTCCCCTT ATTTAGGCTA CATTAACGCT 1020 GATTTTAAAG CTAAAAGCAT TTATATTACC GGGACAATTG GAAGTAGTAA CGCTTTTGAA 1080 AGCGGAGGGA GCGCGGATGT AACCTTTCAA AGCGCTAATA ACTTAGTGTT GAATAAAGCT 1140 AACATAGAAG CTCAAGCCAC AGACAATATC TTTAATCTTT TGGGTCAAGA AGGGATTGAT 1200 AAAATCTTTA ATCAGGGGAA TTTAGCGAAT GTTCTTAGTC AAATGGCTAT GGAAAAAATC 1260 AAGCAAGCCG GCGGTTTAGG GAACTTTATA GAAAACGCTC TAAGCCCTTT GAGTAAGGAA 1320 TTACCCGCTA GCTTGCAAGA TGAAACCTTA GGCCAACTTA TAGGTCAAAA TAACTTAGAT 1380 GATTTATTGA ATAATAGTGG AGTCATGAAT GAAATCCAAA ACATTATCAG TCAAAAACTA 1440 AGCATTTTTG GCAATTTTGT TACCCCATCC ATCATAGAAA ACTACCTTGC TAAGCAGTCT 1500 TTAAAAAGCA TGCTAGACGA TAAAGGGCTT TTGAATTTTA TCGGTGGGTA TATAGACGCT 1560 TCTGAATTAA GCTCTATTTT AGGCGTGATT TTAAAGGATA TTACTAACCC CCCTACAAGC 1620 CTGCAAAAAG ACATTGGTGT GGTAGCGAAC GACTTGTTGA ACGAGTTTTT AGGACAAGAT 1680 GTTGTCAAAA AGCTAGAAAG TCAAGGCTTG GTGAGTAATA TCATCAATAA TGTTATTTCT 1740 CAAGGCGGGT TGAGCGGCGT TTATAATCAA GGTTTAGGGA GCGTGTTGCC GCCCTCTTTA 1800 CAAAACGCGC TCAAAGAAAA CGATTTAGGC ACTCTTTTAT CGCCTAGAGG CTTGCATGAT 1860 TTTTGGCAAA AAGGGTATTT TAACTTTTTA AGCAATGGCT ATGTTTTTGT CAATAACAGC 1920 TCTTTTAGTA ACGCTACTGG GGGTAGTTTG AATTTTGTCG CCAACAAGTC TATTATCTTT 1980 AATGGCGATA ATACGATTGA CTTTAGCAAG TATCAAGGCG CATTGATTTT TGCTTCTAAT 2040 GGTGTTTCTA ATATCAATAT CACCACCCTA AACGCCACTA ATGGCTTAAG CCTTAATGCG 2100 GGTTTGAATA ATGTGAGCGT TCAAAAAGGA GAAATTTGTA TCAATTTAGC CAATTGCCCT 2160 ACAACCAAAA ACAGCTCTCC TGCAAACTCT AGCGTAACCC CCACTAATGA GTCTTTAAGC 2220 GTGCACGCTA ATAATTTCAC TTTCTTAGGC ACAATCATCT CTAATGGGGC TATTGATTTG 2280 TCTCAAGTAA CAAATAATAG CGTTATAGGC ACGCTCAATC TCAATGAAAA TGCGACCTTG 2340 CAAGCTAATA ATTTAACGAT CACCAACGCT TTTAACAACG CCTCTAACTC TACGGCTAAT 2400 ATTGATGGTA ATTTCACCTT AAACCAACAA GCGACTTTAA GCACTAACGC TAGTGGTTTG 2460 AATGTCATGG GGAATTTTAA TAGCTATGGC GATTTGGTGT TTAACCTCAG TCATTCAGTT 2520 AGTCATGCTA TTATCAATAC TCAAGGCACA GCGACGATCA TGGCCAATAA TAACCCTTTG 2580 ATCCAATTCA ACGCTTCTTC AAAAGAAGTG GGTACTTACA CGCTGATTGA TAGCGCTAAA 2640 GCCATTTATT ACGGGTATAA CAACCAAATC ACAGGAGGCA GTAGCCTGGA TAATTACCTT 2700 AAGCTTTATG CGCTCATTGA TATTAATGGC AAGCACATGG TGATGACTGA CAACGGCTTA 2760 ACCTATAACG GGCAAGCCGT GAGCGTTAAA GATGGCGGTT TAGTTGTAGG CTTTAAGGAC 2820 TCTCAAAATC AATACATTTA CACTTCCATT CTTTATAATA AAGTGAAAAT CGCTGTTTCT 2880 AATGATCCTA TCAATAACCC ACAAGCCCCC ACTTTAAAAC AATATATCGC TCAAATTCAG 2940 GGCGTTCAAA GCGTGGATAG CATCGATCAA GCTGGGGGAA ATCAAGCGAT TAATTGGCTC 3000 AATAAAATCT TTGAAACTAA AGGAAGCCCT TTATTCGCTC CCTATTATCT AGAGAGCCAC 3060 TCCACAAAAG ATTTAACCAC GATCGCTGGA GATATTGCTA ACACTTTAGA AGTCATCGCT 3120 AACCCTAATT TTAAAAATGA CGCCACTAAT ATTTTACAGA TCAACACCTA CACGCAGCAA 3180 ATGAGTCGTT TAGCCAAGCT CTCTGACACT TCAACTTTCG CCCGTTCTGA TTTCTTAGAA 3240 CGCTTAGAAG CCCTTARAAA CAAGCGATTC GCTGATGCGA TCCCTAACGC TATGGATGTG 3300 ATTTTAAAAT ACTCTCAAAG GAATAGAGTT ARAAATAATG TGTGGGCGAC AGGAGTTGGA 3360 GGGGCTAGTT TCATTAGTGG AGGTACTGGA ACTTTATATG GTATCAATGT AGGGTATGAT 3420 AGGTTTATTA AGGGCGTGAT TGTGGGAGGT TATGCCGCTT ATGGGTATAG CGGGTTCCAT 3480 GCAAACATCA CTCAATCAGG CTCTAGCAAT GTCAATGTGG GCGTTTATAG CCGAGCGTTT 3540 ATCAAAAGAA GCGAGCTAAC CATGAGCTTG AATGAGACTT GGGGATACAA TAAAACTTTC 3600 ATCAACTCCT ATGACCCCCT ACTCTCAATC ATCAATCAGT CTTACAGATA CGACACTTGG 3660 ACGACTGACG CTAAAATCAA TTATGGCTAT GATTTCATGT TTAAAGATAA AAGCGTTATT 3720 TTTAAACCCC AAGTAGGCTT AAGCTATTAT TACATTGGTT TGTCTGGTTT AAGGGGCATT 3780 ATGGATGATC CTATTTACAA CCAATTCAGA GCCAATGCTG ACCCTAATAA AAAATCCGTT 3840 CTAACGATCA ATTTTGCCCT AGAAAGTCGG CATTATTTCA ATAAAAACTC TTATTATTTT 3900 GTGATTGCGG ATGTGGGCAG AGACTTATTC ATTAATTCTA TGGGGGATAA AATGGTGCGT 3960

TTCATCGGTA ATAACACCCT AAGCTATAGA GATGGTGGCA GATACAACAC TTTTGCTAGC 4020 ATTATCACAG GCGGGGAGAT AAGATTGTTC AAAACCTTTT ATGTGAATGC GGGCATAGGG 4080 GCTAGGTTTG GGCTTGATTA TAAAGATATT AATATTACCG GAAATATTGG TATGCGCTAT 4140 GCTTTTTAA 4149 (2) INFORMATION FOR SEQ ID NO:59: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 789 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...789 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:59: ATGAAAAAAA TTGGTTTGAG CTTGTGTTTG GTTTTGAGTT TGGGTTTTTT AAAAGCCCAT 60 GAAGTGAGCG CTGAAGAGAT TGCGGATATT TTCTACAAAC TCAACGCCAA AGAGCCTAAA 120 ATGAAAATCA ACCACACGAA GGGGTTTTGC GCTAAAGGCG TGTTCCTCCC TAACCCGCAA 180 GCAAGAGAGG ATTTAGAGGT GCCACTACTC AATGAAAAAG AAATCCCTGC GTCTGTAAGG 240 TATTCTTTAG GGGGCGTGGC GATGGACGAT AAAAGCAAGG TTAGGGGAAT GGCGTTAAAA 300 CTAGAAAATC AAAACGCTAG TTGGACAATG GTGATGCTCA ATACAGAAAT CAATTTTGCC 360 AAAAACCCTG AAGAATTCGC CCAATTTTTT GAAATGAGAC TTCCTAAAAA TGGCAAGGTA 420 GATGAAGCAA GAATCAAAAA GCTTTACGAA GAAGTCCCCT CTTATAGGAA TTTTGCCGCC 480 TATATGAAAA CGATAGGGAT TAGCTCAAGC GTGGCTAATA CGCCTTATTA TAGCGTGCAT 540 GCGTTCAAGT TTAAAGATAA GAAAGAAAAA TTATTGCCTG CGAGGTGGAA ATTTGTGCCT 600 AAAGAGGGCG TTAAATACTT AAATCCTCAA GAATTAAAGC AAAAAGATTC AAATTATCTG 660 CTCTCTTCAT TCCAACAACA CCTTAAAAAT AAACCCATAG AATACCAAAT GTATTTGGTG 720 TTTGCGAATC AAAATGATGC CACCAACGAC ACGACCGCGC TTTGGAAAGG CAGCATAAGG 780 AATTATTAG 789 (2) INFORMATION FOR SEQ ID NO:60: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 741 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO

(iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...741 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:60: ATGAAACAAT TTAAAAAGAA ACCAAAAAAG ATAAAACGAT CGCATCAAAA TCAAAAAACA 60 ATCTTAAAGC GTCCTTTATG GCTTATGCCT TTACTGATTG GCGGGTTTGC TAGTGGGGTG 120 TATGCGGATG GAACAGACAT TTTGGGGCTT AGTTGGGGGG AAAAAAGCCA AAAGGTATGC 180 GTGCATCGTC CATGGTATGC TATATGGAGT TGCGATAAAT GGGAGGAAAA AACACAACAA 240 TTTACAGGAA ACCAACTCAT CACAAAAACT TGGGCAGGGG GTAATGCGGC TAACTACTAC 300 CACTCTCAAA ACAACCAAGA CATCACAGCC AATTTAAAAA ATGATAACGG CACTTATTTT 360 TTAAGCGGTC TGTATAACTA CACCGGAGGG GAATATAATG GGGGGAATTT AGACATTGAA 420 TTAGGCAGTA ACGCTACTTT TAATCTAGGT GCGAGTAGTG GGAATAGCTT CACTTCTTGG 480 TATCCTAATG GGCATACTGA TGTTACTTTT AGCGCTGGGA CTATCAATGT GAATAACAGC 540 GTAGAAGTGG GCAATCGTGT GGGATCGGGA GCTGGCACGC ACACCGGCAC AGCCACTTTA 600 AACTTGAACG CTAATAAGGT TACTATCAAT TCCAATATCA GCGCGTATAA AACTTCGCAA 660 GTGAATGTAG GCAATGCTAA CAGCGTTATT ACCATTAATT CGGTTTCTTT AAATGGGGAA 720 TACTTGCAGT TCTTTAGCTA G 741 (2) INFORMATION FOR SEQ ID NO:61: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 738 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...738 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:61: ATGATAAAAA AGACCCTTGC ATCGGTTTTA TTAGGATTGA GTTTGATGAG TGTGTTAAAT 60 GCCAAAGAAT GCGTTTCGCC CATAACAAGA AGCGTTAAGT ATCATCAGCA AAGTGCTGAG 120 ATCAGAGCCT TGCAATTACA AAGTTACAAA ATGGCGAAAA TGGCGCTAGA CAATAACCTT 180 AAGCTCGTTA AAGACAAAAA GCCAGCCGTC ATCTTGGATT TAGATGAAAC CGTTTTGAAC 240 ACTTTTGATT ATGCGGGCTA TTTAGTCAAA AACTGCATTA AATACACCCC AGAAACTTGG 300 GATAAATTTG AAAAAGAAGG CTCTCTTACG CTCATTCCTG GAGCGCTAGA CTTTTTAGAA 360 TACGCTAATT CTAAGGGCGT TAAGATTTTT TACATTTCTA ACCGCACCCA AAAAAATAAG 420

GCATTCACTT TAAAAACGCT CAAAAGCTTT AAGCTCCCCC AAGTGAGTGA AGAATCCGTT 480 TTGTTAAAGG AAAAAGGCAA GCCTAAAGCC GTTAGGCGGG AGTTAGTCGC TAAGGATTAT 540 GCGATTGTTT TACAAGTGGG CGACACTTTG CATGATTTTG ACGCCATTTT TGCTAAAGAC 600 GCTAAAAACA GCCAAGAACA ACAAGCCAAA GTCTTGCAAA ACGCTCAAAA ATTCGGCACA 660 GAATGGATCA TTTTACCCAA CTCTCTTTAT GGCACATGGG AAGATGGGCC TATAAAAGCA 720 TGGCAAAATA AAAAATAA 738 (2) INFORMATION FOR SEQ ID NO:62: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 867 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...867 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:62: TTGTGGTGTT TAAAAACCCC TATCATAGGG CATGGCATGA AGAAAAAAGC AAAAGTCTTT 60 TGGTGTTGTT TTAAAATGAT TCGTTGGTTG TATTTGGCGG TCTTTTTTTT GTTGAGCGTA 120 TCAGACGCTA AAGAAATCGC TATGCAACGA TTTGACAAAC AAAACCATAA GATTTTTGAA 180 ATCCTTGCGG ATAAAGTGAG CGCCAAAGAC AATGTGATAA CCGCCTCAGG GAATGCGATC 240 CTATTGAATT ATGACGTGTA TATTCTAGCG GATAAGGTGC GTTATGACAC CAAGACTAAA 300 GAAGCGTTAT TAGAAGGCAA TATTAAGGTT TATAGGGGCG AGGGCTTGCT CGTTAAAACC 360 GATTATGTGA AATTGAGTTT GAACGAAAAA TATGAGATCA TTTTCCCCTT TTATGTCCAA 420 GACAGCGTGA GCGGGATTTG GGTGAGCGCG GATATTGCTA GCGGGAAGGA TCAAAAATAT 480 AAGATTAAAA ACATGAGCGC TTCAGGGTGC AGCATTGACA ACCCCATTTG GCATGTCAAT 540 GCGACTTCAG GCTCATTTAA CATGCAAAAA TCGCATTTGT CAATGTGGAA TCCTAAGATT 600 TATGTCGGCG ATATTCCTGT ATTGTATTTG CCCTATATTT TCATGTCCAC GAGCAATAAA 660 AGAACTACCG GGTTTTTATA CCCTGAGTTT GGCACTTCCA ACTTAGACGG CTTTATTTAT '720 TTGCAACCCT TTTATTTAGC CCCCAAAAAC TCATGGGATA TGACCTTTAC CCCACAAATC 780 CGTTACAAAA GGGGTTTTGG CTTGAATTTT GAAGCGCGCT ACATCAACTC TAAGACGCAG 840 GTTTTTATTC AATGCGCGCT ATTTTAG 867 (2) INFORMATION FOR SEQ ID NO:63: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 387 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic)

(iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...387 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:63: TTGATGTTTA AAAAAATGTG TTTGAGCCTG CTAATGATAA GCGGTGTTTG TGTGGGGGCA 60 AAGGATTTGG ATTTCAAGCT GGATTATCGC GCGACTGGGG GGAAATTCAT GGGGAAAATG 120 ACGGACTCTA GTCTTTTAAG TATCACTTCT ATGAACGATG AACCGGTGGT GATTAAAAAC 180 CTTATTGTCA ATAGGGGAAA TTCATGCGAA GCGACTAAAA AAGTAGAACC CAAATTTGGC 240 GATAAGTTTA AAAAAGAAAA ACTCTTTGAT CATGAATTAA AATACTCGCA ACAGATATTT 300 TACCGCCTGG ATTGCAAGCC TAACCAATTG TTAGAAGTTA AAATCATCAC GGACAAGGGC 360 GAATATTACC ATAAATTTTC CAAATAG 387 (2) INFORMATION FOR SEQ ID NO:64: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 510 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...510 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:64: ATGCAAGCGT TAAAATCATT GCTTGAAGTG ATTACAAAAC TCCAGAATCT AGGCGGCTAT 60 TTGATGCATA TAGCTATTTT CATCATTTTT ATTTGGATTG GAGGGCTTAA GTTTGTGCCT 120 TACGAAGCTG AAGGGATCGC CCCTTTTGTG GCCAACTCCC CTTTCTTTTC TTTCATGTAT 180 AAATTTGAAA AACCTGCATA CAAACAACAC AAAATGTCTG AATCCCAATC CATGCAAGAA 240 GAAATGCAAG ATAACCCTAA AATCGTTGAA AACAAAGAAT GGCATAAAGA AAACCGCACT 300 TATTTAGTGG CTGAAGGTTT AGGGATTACG ATCATGATCC TAGGCATTTT GGTGCTTTTG 360 GGGCTTTGGA TGCCTTTAAT GGGCGTAGTT GGGGGCTTGC TTGTCGCTGG AATGACGATC 420 ACCACCCTAT TCTTTTTTAT TCACAACGCC AGAAGTGTTT GTCAATCAGC ATTTCCCATG 480 GCTTTCTGGG GCTGGAAGGC TAGTGGTTAA 510

(2) INFORMATION FOR SEQ ID NO:65: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1464 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...1464 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:65: ATGATTGAAT GGATGCAAAA TCATAGAAAG TATTTAGTGG TTACGATATG GATAAGCACG 60 ATCGCTTTTA TTGCCGCCGG AATGATAGGT TGGGGGCAAT ACAGCTTTTC TTTAGATAGC 120 GATAGCGCTG CCAAAGTGGG ACAGATTAAG ATTTCTCAAG AAGAATTAGC CCAAGAATAC 180 CGCCGCCTTA AAGACGCCTA TGCTGAGTCT ATCCCTGATT TTAAAGAACT CACCGAAGAT 240 CAAATCAAAG CCATGCATTT AGAAAAAAGC GCGCTAGATT CGCTCATCAA TCAAGCTTTA 300 TTGAGGAATT TCGCTTTAGA TTTAGGGCTT GGTGCTACCA AGCAAGAAGT GGCCAAAGAG 360 ATCAGAAAAA CGAACGTTTT TCAAAAAGAT GGCGTTTTTG ATGAAGAATT GTATAAAAAT 420 ATCTTAAAAC AAAGCCATTA CCGCCCCAAG CATTTTGAAG AAAGCGTTGA AAGGCTTTTA 480 ATCCTTCAAA AAATCAGCGC TCTATTCCCC AAAACCACCA CCCCTTTGGA GCAATCCAGT 540 CTATCGCTTT GGGCAAAATT GCAAGACAAA TTAGACATTC TTATCCTAAA TCCTAATGAT 600 GTTAAAATCT CTCTCAATGA AGAAGAGATG AAAAAATATT ATGAAAACCA TAGAAAGGAT 660 TTTAAAAAGC CCACAAGCTT TAAAACACGC TCTTTATATT TTGACGCTAG TTTAGAAAAA 720 ACTGATTTGA AAGAGTTGGA GGAATACTAC CATAAAAACA AGGTGTCTTA TTTGGACAAA 780 GAGGGGAAAT TACAGGATTT TAAARGCGTT CAAGAGCAAG TCAAGCATGA TTTAAACATG 840 CAAAAGGCGA ATGAAAAAGC CTTAAGGAGC TATATCGCTC TAAAAAAGGG GAACGCACAA 900 AACTACACCA CGCAAGATTT TGAAAAAAAC AACTCCCCCT ATACTGCTGA AATCACGCAA 960 AAACTCACCG CTCTCAAGCC CCTTGAAGTC CTAAAACCAG AGCCTTTTAA AGATGGTTTT 1020 ATCGTGGTGC AGCTTGTCTC TCAAATTAAA GACGAATTGC AAAATTTTGA TGAAGCCAAA 1080 AGCGCTCTTA AAACCCGTCT GACTCAAGAA AAAACCCTTA TGGCGTTGCA AACTTTAGCT 1140 AAAGAAAAGC TTAAGGATTT TAAAGGGAAA AGCGTGGGTT ATGTAAGCCC TAATTTTGGA 1200 GGCACTATCA GTGAACTTAA CCAAGAAGAG AGCGCGAAGT TTATCAACAC CCTTTTTAAC 1260 CGCCAGGAAA AAAAAGGGTT TGTAACCATA GGTAATAAAG TGGTGCTTTA TCAAATCACA 1320 GAGCAAAATT TCAATCACCC CTTTAGTGCA GAAGAAAACC AATACATGCA GCGTTTAGTC 1380 AATAACACTA AAACGGATTT TTTTGATAAA GCGTTGATAG AAGAATTGAA AAAACGCTAT 1440 AAGATAGTCA AATACATTCA ATAA 1464 (2) INFORMATION FOR SEQ ID NO:66: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 429 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double

(D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...429 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:66: ATGAAAACGA ACTTTTATAA AATTAAATTA CTATTTGCTT GGTGTCTTAT CATTGGCATG 60 TTTAACGCTC CGCTTAACGC TGACCAAAAC ACGGATATAA AAGATATTAG TCCTGAAGAT 120 ATGGCGCTAA ATAGCGTGGG GCTTGTTTCT AGAGATCAGC TAAAAATAGA GATCCCTAAA 180 GAAACCCTAG AGCAAAAAGT GGCCATACTC AATGACTATA ATGATAAGAA TGTTAATATC 240 AAGTTTGACG ACATAAGTTT AGGGAGTTTC CAACCTAATG ATAATCTAGG TATCAATGCG 300 ATGTGGGGCA TTCAAAATCT TCTCATGAGC CAAATGATGA GCAATTACGG TCCAAACAAT 360 TCTTTCATGT ATGGCTATGC GCCAACATAC TCAGATTCAT CGTTTTTACC ACCGATCTTA 420 GGGTATTAA 429 (2) INFORMATION FOR SEQ ID NO:67: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 627 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...627 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:67: TTGATCAACA ATAATAATAA CAATAAAAAA CTGAGAGGCT TTTTTTTGAA AGTTCTCTTA 60 AGTCTCGTTG TTTTCAGTTC GTATGGGTCA GCAAATGACG ATAAAGAAGC CAAAAAAGAA 120 GCGCTAGAAA AAGAAAAAAA CACTCCCAAT GGGCTTGTTT ATACGAATTT AGATTTTGAT 180 AGTTTTAAAG CGACTATCAA AAATTTGAAA GACAAGAAAG TAACTTTCAA AGAAGTCAAT 240 CCCGATATTA TCAAAGATGA AGTTTTTGAC TTCGTGATTG TCAATAGAGT CCTTAAAAAA 300 ATAAAGGATT TGAAGCATTA CGATCCAGTT ATTGAAAAAA TCTTTGATGA AAAGGGTAAA 360

GAAATGGGAT TGAATGTAGA ATTACAGATC AATCCTGAAG TGAAAGACTT TTTTACTTTC 420 AAAAGCATCA GCACGACCAA CAAACAACGC TGCTTTCTAT CATTGCACGG AGAAACAAGA 480 GAAATTTTAT GCGATGATAA GCTATATAAT GTTTTATTGG CCGTATTCAA TTCTTATGAT 540 CCTAATGATC TTTTGAAACA CATTAGCACC ATAGAGTCTC TCAAAAAAAT CTTTTATACG 600 ATTACATGTG AAGCGGTATA TCTATAA 627 (2) INFORMATION FOR SEQ ID NO:68: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 738 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...738 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:68: ATGGCAGGCA CACAAGCTAT ATATGAATCA TCTTCTGCAG GATTCTTATC GCAAGTCTCC 60 TCAATCATCT CAAGCACAAG TGGTGTCGCA GGGCCATTTG CAGGAATAGT AGCGGGCGCT 120 ATGACAGCAG CGATTATTCC TATTGTTGTG GGATTTACTA ATCCGCAAAT GACCGCTATC 180 ATGACCCAAT ACAATCAAAG CATCGCTGAA GCTGTAAGCG TGCCTATGAA AGCCGCTAAC 240 CAACAATACA ACCAATTGTA TCAAGGTTTT AACGATCAAA GCATGGCTGT GGGGAACAAT 300 ATCTTAAATA TCAGCAAATT AACAGGGGAA TTTAACGCGC AAGGCAACAC GCAAAGCGCG 360 CAAATTAGTG CTGTCAATAG TCAGATTGCA AGCATTTTAG CGAGTAACAC TACCCCTAAA 420 AATCCTAGCG CTATTGAAGC TTATGCGACG AATCAAATCG CTGTTCCTAG CGTGCCAACA 480 ACGGTTGAAA TGATGAGCGG TATATTAGGC AATATTACAA GCGCAGCACC AAAATACGCC 540 CTAGCTCTAC AAGAGCAACT GCGTTCTCAA GCAAGCAACA GCTCAATGAA TGATACAGCC 600 GATTCCCTTG ATAGCTGTAC CGCTTTAGGC GCACTTGTTG GCTCATCAAA AGTGTTTTTC 660 AGTTGCATGC AAATTTCTAT GACTCCTATG AGTGTTTCTA TGCCCACTGT TATGCCAAAT 720 ACCAGCGGTT GCCACTAA 738 (2) INFORMATION FOR SEQ ID NO:69: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1104 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO

(iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...1104 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:69: ATGATTAAAA GCGTAGAGAT TGAAAATTAC AAAAATTTTG AGCACCTTAA AATGGAAAAT 60 TTTAAACTCA TCAACTTTTT TACCGGTCAA AACGATGCGG GTAAAACCAA TCTTTTAGAA 120 GCTCTTTATA CCAACACAGG CCTTTGTGAT CCTACTGCCA ATCAAGTCAG TCTTCCTCCT 180 GAACATGCCG TGAATATTAG TGAATTCAGA AAAATCAAAC TCGATGCCGA CAACCTAAAA 240 ACCTTTTTTT ATCAAGGAAA CACCGCTAAT CCCATTAGTA TCCGCACTGA ATTTGAACAT 300 GCTACTATCC CTCTTACTAT CCAATACCCC ACACAAACCA GTTACAGCAA AGACATCAAT 360 TTGAATAGCG ATGATGCTCA TATGACAAAC CTTATAAACA CAACAATAAC GAAGCCACAG 420 CTCCAATTTT CCTACAATCC ATCCCTTTCC CCCATGACAA TGACTTATGA ATTTGAAAGG 480 CAAAACCTAG GTTTAATCCA TTCTAATTTA GATA AATCG CTCAAACCTA TAAAGAAAAT 540 GCGATGTTTA TTCCTATAGA ATTATCTATT GTTAATTCTC TTAAAGCATT GGAAAATTTA 600 CAATTAGCAA GCAAAGAAAA AGAATTGATT GAAATCCTAC AATGTTTCAA CCCTAATATT 660 TTAAATGCTA ATACAATAAG AAAGTCTGTC TATATCCAAA TCAAAGATGA AAACACACCG 720 CTAGAAGAAA GTCCCAAAAG GCTTTTAAAT TTGTTTGGTT GGGGTTTTAT CAAATTCTTT 780 ATTATGGTGA GCATTCTTAT AGACAATCGT GTCAAGTATC TTTTTATTGA TGAAATAGAA 840 AGCGGTTTGC ACCATACAAA AATGCAAGAG TTTTTAAAAG CTCTGTTTAA GTTAGCTCAA 900 AAATTACAGA TTCAAATTTT TGCCACCACG CACAATAAGG AATTTTTATT AAACGCCATC 960 AACACGATAT CCGATAATGA AACGGGAGTT TTTAAAGACA TAGCCTTGTT TGAGCTTGAA 1020 AAAGAAAGCG CTTCTGGCTT TATCAGACAC AGCTATTCTA TGCTAGAAAA AGCGCTTTAT 1080 AGGGGTATGG AGGTTAGAGG CTGA 1104 (2) INFORMATION FOR SEQ ID NO:70: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1230 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...1230 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:70: ATGTCCTTGA TTAGAGTGAA TGGGGAAGCT TTTAAACTCT CTTTGGAAAG TTTAGAAGAA 60

GATCCTTTTG AAACTAAAGA AACGCTAGAA ACGCTAGAAA CGCTTATCAA ACAAACGAGC 120 GTTGTTTTAT TGGCCGCTGG GGAGTCTAAG CGTTTTTCTC GTGCGATTAA AAAGCAGTGG 180 CTACGCTCTC ACCACACCCC CTTATGGCTC AGCGTGTATG AAAGCTTTAA AGAAGCCCTA 240 GACTTTAAGG AAGTCATTCT AGTTGTAAGC GAATTGGATT ATGTTTATAT CCAACGCCAT 300 TACCCCAAAA TCAAGCTTGT AAAAGGCGGG GCATCAAGGC AAGAATCCGT GCGTAACGCT 360 TTGAAAGTAA TTGATAGCAC TTACACGATC ACCAGCGATG TGGCTAGGGG TTTAGCGAAT 420 ATGGAAGCGC TTAAAAGCTT GTTTTTAACC CTCCAACAAA CGAGCCATTA TTGCATCGCC 480 CCTTACTTGC CTTGCTATGA CACAGCGATC TATTATAACG AGGCTTTAGA TAGAGAAGCG 540 ATCAAACTCA TTCAAACCCC GCAATTAAGC CACACCAAAA CGCTCCAATC AGCCCTAAAC 600 CAAGGGGGTT TTAAAGATGA AAGCAGCGCG ATTTTACAAG CTTTCCCTAA CTCTGTGAGC 660 TATATTGAAG GCAGTAAGGA TTTGCACAAA CTCACCACAA GCGGCGATTT AAAGTTTTTT 720 ACGCCTTTTT TTAACCCAGC AAAGGACACT TTTATAGGCA TGGGTTTTGA TACGCATGCG 780 TTCATTAAAG ATAAGCCTAT GGTTTTAGGG GGGGTTGTTT TGGATTGCGA GTTTGGGTTA 840 AAGGCTCATA GCGATGGCGA TGCTTTATTG CATGCGGTTA TTGATGCGAT TTTAGGAGCG 900 ATTAAAGGGG GGGATATTGG CGAATGGTTC CCTGATAATG ACCCCAAATA CAAAAACGCC 960 TCTTCTAAAG AGCTTTTAAA AATCGTGTTG GATTTTTCTC AAAGCATTGG GTTTGAATTG 1020 CTTGAAATGG GAGCGACCAT CTTTAGCGAA ATCCCTAAAA TCACTCCTTA CAAACCGGCG 1080 ATTTTAGAGA ATTTGAGCCA ACTTTTGGGT TTAGAAAAAT CTCAAATCAG CTTGAAAGCC 1140 ACTACAATGG AAAAAATGGG GTTCATTGGC AAACAAGAAG GGCTGTTAGT CCAAGCGCAT 1200 GTGAGCATGC GTTATAAACA AAAACTTTAA 1230 (2) INFORMATION FOR SEQ ID NO:71: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 813 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...813 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:71: ATGAAAAAGT TTGTAGCTTT AGGGCTTCTA TCCGCGGTTT TAAGCTCTTC GTTGTTAGCC 60 GAAGGTGATG GTGTTTATAT AGGGACTAAT TATCAGCTTG GACAAGCCCG TTTGAATAGC 120 AATATTTATA ATACAGGGGA TTGCACAGGG AGTGTTGTAG GTTGCCCCCC AGGTCTTACC 180 GCTAATAAGC ATAATCCAGG AGGCACCAAT ATCAATTGGC ACTCCAAATA CGCTAATGGG 240 GCTTTGAATG GTTTTGGGTT GAATGTGGGT TATAAGAAAT TCTTCCAATT CAAGTCGCTA 300 GATATGACAA GCAAGTGGTT TGGTTTTAGA GTGTATGGGC TTTTTGATTA CGGGCATGCC 360 GATTTAGGTA AACAAGTTTA TGCACCTAAT AAAATCCAGT TGGATATGGT CTCTTGGGGT 420 GTGGGGAGCG ATTTGTTAGC TGATATTATT GATAAAGACA ACGCTTCTTT TGGTATTTTT 480 GGTGGGGTCG CTATCGGCGG TAACACTTGG AAAAGCTCTG CAGCAAACTA TTGGAAAGAG 540 CAAATCATTG AAGCCAAAGG TCCTGATGTT TGTACCCCTA CTTATTGTAA CCCTAATGCC 600 CCTTATAGCA CCAACACTTC AACCGTCGCT TTTCAAGTGT GGTTGAATTT TGGGGTGAGA 660

GCCAATATCT ACAAGCATAA TGGCGTGGAA TTTGGCGTGA GAGTGCCGCT ACTCATCAAT 720 AAATTTTTGA GCGCGGGTCC TAACGCTACT AACCTTTATT ACCATTTGAA ACGGGATTAT 780 TCGCTTTATT TGGGGTATAA CTACACTTTT TAA 813 (2) INFORMATION FOR SEQ ID NO:72: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1317 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...1317 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:72: ATGGCTTACA AACCTAACAA AAAGAAGTTA AAAGAATTAA GAGAGCAACC GAATTTATTT 60 AGCATCTTAG ATAAGGGCGA TGTTGCAACA AACAATCCTG TTGAAGAGTC AGACAAGGCC 120 AATAAAATAC AAGAGCCACT CCCTTATGTC GTGAAAACGC AAATCAATAA AGCAAGCATG 180 ATTTCTAGAG ATCCTATTGA ATGGGCAAAG TATTTAAGCT TTGAAAAACG AGTCTATAAG 240 GATAATAGTA AAGAAGATGT CAATTTCTTT GCCAATGGTG AGATAAAAGA AAGTTCTCGT 300 GTTTATGAAG CGAATAAAGA AGGGTTTGAA AGGCGCATCA CTAAAAGATA CGATCTGATT 360 GATAGAAATA TTGATAGAAA TAGAGAATTT TTTATAAAAG AAATTGAAAT TCTAACCCAC 420 ACAAACAGCT TAAAAGAATT GAAAGAGCAA GGGTTAGAAA TCCAATTGAC CCACCATAAT 480 GAAACGCATA AGAAAGCCTT AGAAAATGGC AATGAAATCG TTAAAGAATA CGACCATCTT 540 AAAGATATTT ACCAAGAAGT AGAAAGAACA AAAGATGGTG GATTGGTAAG AGAAATAATC 600 CCCAGTATTT CTAGCGCTGA GTATTTCAAG CTTTACAACA AACTGCCTTT TGAATCAATA 660 AACAATGAAA ATACCAAACT GAATACTAAC GACAATGAAG AAGTTAAAAA ACTAGAATTT 720 GAATTAGCTA AAGAAGTGCA TATTTTAATC CTAGAGCAAC AATTGCTTTC AGCAACAAAT 780 TATTATTCTT GGATAGATAA AGATGATAAT GCGAATTTTG CTTGGAAAAT GCATAGGCTT 840 ATCAATGAAA ATAAACTCAA AGAAAACCAT CTCAGCGCCA ATAACGCTAA TAAGATTAAG 900 CAATTTTTCT TTAATAATGG TTCTATTTTA GGCTGGACTA AAGAAGAACA AAGCGCTATA 960 CAAGAAAACA GAGATTATTC TTTAAGAAGC GCTCTTTTAA GTTTAGAAGA AATCGCTCAA 1020 GCAAAAATTG AATTGCAAAA ATACTATGAA AGCGTTTATG TTAATGGTGA TGGGAATAAA 1080 AGAGAAATCA AGCCTTTTAA AGAAATTTTA AGAGACACCA ACAATTTTGA AAAAGCTTAT 1140 AAGGAGCGTT ATGACAAATT GGTAAGCTTG AGTGCAGCAA TCATTCAAGC TAAAGAGGGT 1200 GGTAATGAGC GACCAAATTC TAGTGCAAAT AACAATAACC CTATTAAAAA TACAATAGAG 1260 ACTAATACTT CTAACAATAT TATTCAAAAT AATGATAATA TAATCATCCA AATTTAA 1317 (2) INFORMATION FOR SEQ ID NO:73: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 648 base pairs (B) TYPE: nucleic acid

(C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...648 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:73: ATGCAAGCGT TAAAATCATT GCTTGAAGTG ATTACAAAAC TCCAGAATCT AGGCGGCTAT 60 TTGATGCATA TAGCTATTTT CATCATTTTT ATTTGGATTG GAGGGCTTAA GTTTGTGCCT 120 TACGAAGCTG AAGGGATCGC CCCTTTTGTG GCCAACTCCC CTTTCTTTTC TTTCATGTAT 180 AAATTTGAAA AACCTGCATA CAAACAACAC AAAATGTCTG AATCCCAATC CATGCAAGAA 240 GAAATGCAAG ATAACCCTAA AATCGTTGAA AACAAAGAAT GGCATAAAGA AAACCGCACT 300 TATTTAGTGG CTGAAGGTTT AGGGATTACG ATCATGATCC TAGGCATTTT GGTGCTTTTG 360 GGGCTTTGGA TGCCTTTAAT GGGCGTAGTT GGGGGCTTGC TTGTCGCTGG AATGACGATC 420 ACCACCCTAT CTTTTTTATT CACAACGCCA GAAGTGTTTG TCAATCAGCA TTTCCCATGG 480 CTTTCTGGGG CTGGAAGGCT AGTGGTTAAA GACTTGGCGT TATTTGCTGG AGGCTTGTTT 540 GTGGCCGGAT TTGATGCGAA ACGCTATTTG GAGGGTAAAG GGTTTTGCTT GATGGACCGC 600 TCATCGGTAG GGATTAAAAC TAAATGCTCT AGCGGGTGTT GCTCTTAA 648 (2) INFORMATION FOR SEQ ID NO:74: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 186 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...186 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:74: Met Ile Lys Arg Ile Ala Cys Ile Leu Ser Leu Ser Ala Ser Leu Ala 1 5 10 15 Leu Ala Gly Glu Val Asn Gly Phe Phe Met Gly Ala Gly Tyr Gln Gln 20 25 30 Gly Arg Tyr Gly Pro Tyr Asn Ser Asn Tyr Ser Asp Trp Arg His Gly

35 40 45 Asn Asp Leu Tyr Gly Leu Asn Phe Lys Leu Gly Phe Val Gly Phe Ala 50 55 60 Asn Lys Trp Phe Gly Ala Arg Val Tyr Gly Phe Leu Asp Trp Phe Asn 65 70 75 80 Thr Ser Gly Thr Glu His Thr Lys Thr Asn Leu Leu Thr Tyr Gly Gly 85 90 95 Gly Gly Asp Leu Ile Val Asn Leu Ile Pro Leu Asp Lys Phe Ala Leu 100 105 110 Gly Leu Ile Gly Gly Val Gln Leu Ala Gly Asn Thr Trp Met Phe Pro 115 120 125 Tyr Asp Val Asn Gln Thr Arg Phe Gln Phe Leu Trp Asn Leu Gly Gly 130 135 140 Arg Met Arg Val Gly Asp Arg Ser Ala Phe Glu Ala Gly Val Lys Phe 145 150 155 160 Pro Met Val Asn Gln Gly Ser Lys Asp Val Gly Leu Ile Arg Tyr Tyr 165 170 175 Ser Trp Tyr Val Asp Tyr Val Phe Thr Phe 180 185 (2) INFORMATION FOR SEQ ID NO:75: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 116 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...116 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:75: Leu Met Arg Ile Ile Ile Arg Leu Leu Ser Phe Lys Met Asn Ala Phe 1 5 10 15 Leu Lys Leu Ala Leu Ala Ser Leu Met Gly Gly Leu Trp Tyr Ala Phe 20 25 30 Asn Gly Glu Gly Ser Glu Ile Val Ala Ile Gly Ile Phe Val Leu Ile 35 40 45 Leu Phe Val Phe Phe Ile Arg Pro Val Ser Phe Gln Asp Pro Glu Lys 50 55 60 Arg Glu Glu Tyr Ile Glu Arg Leu Lys Lys Asn His Glu Arg Lys Met 65 70 75 80 Ile Leu Gln Asp Lys Gln Lys Glu Glu Gln Met Arg Leu Tyr Gln Ala 85 90 95 Lys Lys Glu Arg Glu Ser Arg Gln Lys Gln Asp Leu Lys Glu Gln Met 100 105 110 Lys Lys Tyr Ser

115 (2) INFORMATION FOR SEQ ID NO:76: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 345 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...345 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:76: Met Val Lys His Tyr Leu Phe Met Ala Val Ser Gln Val Phe Phe Ser 1 5 10 15 Phe Phe Leu Val Leu Phe Phe Ile Ser Ser Ile Val Leu Leu Ile Ser 20 25 30 Ile Ala Ser Val Thr Leu Val Ile Lys Val Ser Phe Leu Asp Leu Val 35 40 45 Gln Leu Phe Leu Tyr Ser Leu Pro Gly Thr Ile Phe Phe Ile Leu Pro 50 55 60 Ile Thr Phe Phe Ala Ala Cys Ala Leu Gly Leu Ser Arg Leu Ser Tyr 65 70 75 80 Asp His Glu Leu Leu Val Phe Phe Ser Leu Gly Val Ser Pro Lys Lys 85 90 95 Met Thr Lys Ala Phe Val Pro Leu Ser Leu Leu Val Ser Ala Ile Leu 100 105 110 Leu Ala Phe Ser Leu Ile Leu Ile Pro Thr Ser Lys Ser Ala Tyr Tyr 115 120 125 Gly Phe Leu Arg Gln Lys Lys Asp Lys Ile Asp Ile Asn Ile Arg Ala 130 135 140 Gly Glu Phe Gly Gln Lys Leu Gly Asp Trp Leu Val Tyr Val Asp Lys 145 150 155 160 Thr Glu Asn Asn Ser Tyr Asp Asn Leu Val Leu Phe Ser Asn Lys Ser 165 170 175 Leu Ser Gln Glu Ser Phe Ile Leu Ala Gln Lys Gly Asn Ile Asn Asn 180 185 190 Gln Asn Gly Val Phe Glu Leu Asn Leu Tyr Asn Gly His Ala Tyr Phe 195 200 205 Thr Gln Gly Asp Lys Met Arg Lys Val Asp Phe Glu Glu Leu His Leu 210 215 220 Arg Asn Lys Leu Lys Ser Phe Asn Ser Asn Asp Ala Ala Tyr Leu Gln 225 230 235 240 Gly Thr Asp Tyr Leu Gly Tyr Trp Lys Lys Ala Phe Gly Lys Asn Ala 245 250 255 Asn Lys Asn Gln Lys Arg Arg Phe Ser Gln Ala Ile Leu Val Ser Leu

260 265 270 Phe Pro Leu Ala Ser Val Phe Leu Ile Pro Leu Phe Gly Ile Ala Asn 275 280 285 Pro Arg Phe Lys Thr Asn Trp Ser Tyr Phe Tyr Val Leu Gly Ala Val 290 295 300 Gly Val Tyr Phe Leu Met Val His Val Ile Ser Thr Asp Leu Phe Leu 305 310 315 320 Met Thr Phe Phe Phe Pro Phe Ile Trp Ala Phe Ile Ser Tyr Leu Leu 325 330 335 Phe Arg Lys Phe Ile Leu Lys Arg Tyr 340 345 (2) INFORMATION FOR SEQ ID NO:77: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 276 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...276 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:77: Met Lys Lys Lys Ala Lys Val Phe Trp Cys Cys Phe Lys Met Ile Arg 1 5 10 15 Trp Leu Tyr Leu Ala Val Phe Phe Leu Leu Ser Val Ser Asp Ala Lys 20 25 30 Glu Ile Ala Met Gln Arg Phe Asp Lys Gln Asn His Lys Ile Phe Glu 35 40 45 Ile Leu Ala Asp Lys Val Ser Ala Lys Asp Asn Val Ile Thr Ala Ser 50 55 60 Gly Asn Ala Ile Leu Leu Asn Tyr Asp Val Tyr Ile Leu Ala Asp Lys 65 70 75 80 Val Arg Tyr Asp Thr Lys Thr Lys Glu Ala Leu Leu Glu Gly Asn Ile 85 90 95 Lys Val Tyr Arg Gly Glu Gly Leu Leu Val Lys Thr Asp Tyr Val Lys 100 105 110 Leu Ser Leu Asn Glu Lys Tyr Glu Ile Ile Phe Pro Phe Tyr Val Gln 115 120 125 Asp Ser Val Ser Gly Ile Trp Val Ser Ala Asp Ile Ala Ser Gly Lys 130 135 140 Asp Gln Lys Tyr Lys Ile Lys Asn Met Ser Ala Ser Gly Cys Ser Ile 145 150 155 160 Asp Asn Pro Ile Trp His Val Asn Ala Thr Ser Gly Ser Phe Asn Met 165 170 175 Gln Lys Ser His Leu Ser Met Trp Asn Pro Lys Ile Tyr Val Gly Asp

180 185 190 Ile Pro Val Leu Tyr Leu Pro Tyr Ile Phe Met Ser Thr Ser Asn Lys 195 200 205 Arg Thr Thr Gly Phe Leu Tyr Pro Glu Phe Gly Thr Ser Asn Leu Asp 210 215 220 Gly Phe Ile Tyr Leu Gln Pro Phe Tyr Leu Ala Pro Lys Asn Ser Trp 225 230 235 240 Asp Met Thr Phe Thr Pro Gln Ile Arg Tyr Lys Arg Gly Phe Gly Leu 245 250 255 Asn Phe Glu Ala Arg Tyr Ile Asn Ser Lys Thr Gln Val Phe Ile Gln 260 265 270 Cys Ala Leu Phe 275 (2) INFORMATION FOR SEQ ID NO:78: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 224 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...224 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:78: Met Ile Arg Leu Lys Gly Leu Asn Lys Thr Leu Lys Thr Ser Leu Leu 1 5 10 15 Ala Gly Val Leu Leu Gly Ala Thr Ala Pro Leu Met Ala Lys Pro Leu 20 25 30 Leu Ser Asp Glu Asp Leu Leu Lys Arg Val Lys Leu His Asn Ile Lys 35 40 45 Glu Asp Thr Leu Thr Ser Cys Asn Ala Lys Val Asp Gly Ser Gln Tyr 50 55 60 Leu Asn Ser Gly Trp Asn Leu Ser Lys Glu Phe Pro Gln Glu Tyr Arg 65 70 75 80 Glu Lys Ile Phe Glu Cys Val Glu Glu Glu Lys His Lys Gln Ala Leu 85 90 95 Asn Leu Ile Asn Lys Glu Asp Thr Lys Asp Lys Glu Glu Leu Ala Lys 100 105 110 Lys Ile Lys Glu Ile Lys Glu Lys Ala Lys Val Leu Arg Gln Lys Phe 115 120 125 Met Ala Phe Glu Met Lys Glu His Ser Lys Glu Phe Pro Asn Lys Lys 130 135 140 Gln Leu Gln Thr Met Leu Glu Asn Ala Phe Asp Asn Gly Ala Glu Ser 145 150 155 160 Phe Ile Asp Asp Trp His Glu Arg Phe Gly Gly Ile Ser Arg Glu Asn

165 170 175 Thr Tyr Lys Ala Leu Gly Ile Lys Glu Tyr Ser Asp Glu Gly Lys Ile 180 185 190 Leu Pro Leu Ala Lys Glu Val Ile Leu Asp Asn Ile Lys Lys Ile Leu 195 200 205 Lys Lys Ala Leu Met Ile Leu Asp Asn Pro Tyr Leu Leu Trp Leu Val 210 215 220 (2) INFORMATION FOR SEQ ID NO:79: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 429 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...429 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:79: Met Pro Tyr Ala Leu Arg Lys Arg Phe Phe Lys Arg Leu Leu Leu Phe 1 5 10 15 Phe Leu Ile Val Cys Met Ile Asn Leu His Ala Lys Ser Tyr Leu Phe 20 25 30 Ser Pro Leu Pro Pro Ala His Gln Gln Ile Ile Lys Thr Glu Pro Cys 35 40 45 Ser Leu Glu Cys Leu Lys Asp Leu Met Leu Gln Asn Gln Ile Phe Ser 50 55 60 Phe Val Ser Gln Tyr Asp Asp Asn Asn Gln Asp Glu Ser Leu Lys Thr 65 70 75 80 Tyr Tyr Lys Asp Ile Leu Asn Lys Leu Asn Pro Val Phe Ile Ala Ser 85 90 95 Gln Thr Pro Ala Lys Glu Ser Tyr Glu Pro Lys Ile Glu Leu Ala Ile 100 105 110 Leu Leu Pro Lys Lys Val Val Gly Arg Tyr Ala Ile Leu Val Met Asn 115 120 125 Thr Leu Leu Ala Tyr Leu Asn Thr Arg Asn Asn Asp Phe Asn Ile Gln 130 135 140 Val Phe Asp Ser Asp Glu Glu Ser Pro Glu Lys Leu Glu Glu Thr Tyr 145 150 155 160 Lys Glu Ile Glu Lys Glu Lys Phe Pro Phe Ile Ile Ala Leu Leu Thr 165 170 175 Lys Glu Gly Val Glu Asn Leu Leu Gln Asn Thr Thr Ile Asn Thr Pro 180 185 190 Thr Tyr Val Pro Thr Val Asn Lys Thr Gln Leu Glu Asn His Thr Glu 195 200 205 Leu Ser Leu Ser Glu Arg Leu Tyr Phe Gly Gly Ile Asp Tyr Lys Glu

210 215 220 Gln Leu Gly Met Leu Ala Thr Phe Ile Ser Pro Asn Ser Pro Val Ile 225 230 235 240 Glu Tyr Asp Asp Asp Gly Leu Ile Gly Glu Arg Leu Arg Gln Ile Thr 245 250 255 Glu Ser Leu Asn Val Glu Val Lys His Gln Glu Asn Ile Ser Tyr Lys 260 265 270 Gln Ala Thr Ser Phe Ser Lys Asn Phe Arg Lys His Asp Ala Phe Phe 275 280 285 Lys Asn Ser Thr Leu Ile Leu Asn Thr Pro Thr Thr Lys Ser Gly Leu 290 295 300 Ile Leu Ser Gln Ile Gly Leu Leu Glu Tyr Lys Pro Leu Lys Ile Leu 305 310 315 320 Ser Thr Gln Ile Asn Phe Asn Pro Ser Leu Leu Leu Leu Thr Gln Pro 325 330 335 Lys Asp Arg Lys Asn Leu Phe Ile Val Asn Ala Leu Gln Asn Ser Asp 340 345 350 Glu Thr Leu Ile Glu Tyr Ala Ser Leu Leu Glu Ser Asp Leu Arg His 355 360 365 Asp Trp Val Asn Tyr Ser Ser Ala Ile Gly Leu Glu Met Phe Leu Asn 370 375 380 Thr Leu Asp Pro His Phe Lys Lys Ser Phe Gln Glu Ser Leu Glu Asp 385 390 395 400 Asn Gln Val Arg Tyr His Asn Gln Ile Tyr Gln Ala Leu Gly Tyr Ser 405 410 415 Phe Glu Pro Ile Lys Asn Glu Ser Glu Thr Lys Lys Glu 420 425 (2) INFORMATION FOR SEQ ID NO:80: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 455 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...455 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:80: Val Leu Lys Phe Gln Lys Leu Pro Leu Leu Phe Val Ser Ile Leu Tyr 1 5 10 15 Asn Gln Ser Pro Leu Leu Ala Phe Asp Tyr Lys Phe Ser Gly Val Ala 20 25 30 Glu Ser Val Ser Lys Val Gly Phe Asn His Ser Lys Leu Asn Ser Lys 35 40 45 Glu Gly Ile Phe Pro Thr Ala Thr Phe Val Thr Ala Thr Ile Lys Leu

50 55 60 Gln Val Asp Ser Asn Leu Leu Pro Lys Asn Ile Glu Lys His Ser Leu 65 70 75 80 Lys Ile Gly Val Gly Gly Ile Leu Gly Ala Leu Ala Tyr Asp Ser Thr 85 90 95 Lys Thr Leu Ile Asp Gln Ala Thr His Gln Ile Tyr Gly Ser Glu Leu 100 105 110 Phe Tyr Leu Ile Gly Arg Trp Trp Gly Phe Leu Gly Asn Ala Pro Trp 115 120 125 Lys Asp Ser Leu Ile Glu Ser Asp Ala His Thr Arg Asn Tyr Val Leu 130 135 140 Tyr Asn Ser Tyr Leu Phe Tyr Ser Tyr Gly Asp Lys Phe His Leu Lys 145 150 155 160 Leu Gly Arg Tyr Leu Ser Asn Met Asp Phe Met Ser Ser Tyr Thr Gln 165 170 175 Gly Phe Glu Leu Asp Tyr Lys Ile Asn Ser Lys Ile Ala Leu Lys Trp 180 185 190 Phe Ser Ser Phe Gly Arg Ala Leu Ala Phe Gly Gln Trp Ile Arg Asp 195 200 205 Trp Tyr Ala Pro Ile Val Thr Glu Asp Gly Arg Lys Glu Val Tyr Asp 210 215 220 Gly Ile His Ala Ala Gln Leu Tyr Phe Ser Ser Lys His Val Gln Val 225 230 235 240 Met Pro Phe Ala Tyr Phe Ser Pro Lys Ile Tyr Gly Ala Pro Gly Val 245 250 255 Lys Ile His Ile Asp Ser Asn Pro Lys Phe Lys Gly Leu Gly Leu Arg 260 265 270 Ala Gln Thr Thr Ile Asn Val Ile Phe Pro Val Tyr Ala Lys Asp Leu 275 280 285 Tyr Asp Val Tyr Trp Arg Asn Ser Lys Ile Gly Glu Trp Gly Ala Ser 290 295 300 Leu Leu Ile His Gln Arg Phe Asp Tyr Asn Glu Phe Asn Phe Gly Phe 305 310 315 320 Gly Tyr Tyr Gln Asn Phe Gly Asn Ala Asn Ala Arg Ile Gly Trp Tyr 325 330 335 Gly Asn Pro Ile Pro Phe Asn Tyr Arg Asn Asn Ser Val Tyr Gly Gly 340 345 350 Val Phe Ser Asn Ala Ile Thr Ala Asp Ala Val Ser Gly Tyr Val Phe 355 360 365 Gly Gly Gly Val Tyr Arg Gly Phe Leu Trp Gly Ile Leu Gly Arg Tyr 370 375 380 Thr Tyr Ala Thr Arg Ala Ser Glu Arg Ser Ile Asn Leu Asn Leu Gly 385 390 395 400 Tyr Lys Trp Gly Ser Phe Ala Arg Val Asp Val Asn Leu Glu Tyr Tyr 405 410 415 Val Val Ser Met His Asn Gly Tyr Arg Leu Asp Tyr Leu Thr Gly Pro 420 425 430 Phe Asn Lys Ala Phe Lys Ala Asp Ala Gln Asp Arg Ser Asn Leu Met 435 440 445 Val Ser Met Lys Phe Phe Phe 450 455 (2) INFORMATION FOR SEQ ID NO:81: (i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 282 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...282 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:81: Met Gly Cys Ser Phe Ile Phe Lys Lys Val Arg Val Tyr Ser Lys Met 1 5 10 15 Leu Val Ala Leu Gly Leu Ser Ser Val Leu Ile Gly Cys Ala Met Asn 20 25 30 Pro Ser Ala Glu Thr Lys Lys Pro Asn Asp Ala Lys Asn Gln Gln Pro 35 40 45 Val Gln Thr His Glu Arg Met Thr Thr Ser Ser Glu His Val Thr Pro 50 55 60 Leu Asp Phe Asn Tyr Pro Val His Ile Val Gln Ala Pro Gln Asn His 65 70 75 80 His Val Val Gly Ile Leu Met Pro Arg Ile Gln Val Ser Asp Asn Leu 85 90 95 Lys Pro Tyr Ile Asp Lys Phe Gln Asp Ala Leu Ile Asn Gln Ile Gln 100 105 110 Thr Ile Phe Glu Lys Arg Gly Tyr Gln Val Leu Arg Phe Gln Asp Glu 115 120 125 Lys Ala Leu Asn Val Gln Asp Lys Lys Lys Ile Phe Ser Val Leu Asp 130 135 140 Leu Lys Gly Trp Val Gly Ile Leu Glu Asp Leu Lys Met Asn Leu Lys 145 150 155 160 Asp Pro Asn Ser Pro Asn Leu Asp Thr Leu Val Asp Gln Ser Ser Gly 165 170 175 Ser Val Trp Phe Asn Phe Tyr Glu Pro Glu Ser Asn Arg Val Val His 180 185 190 Asp Phe Ala Val Glu Val Gly Thr Phe Gln Ala Ile Thr Tyr Thr Tyr 195 200 205 Thr Ser Thr Asn Asn Ala Ser Gly Gly Phe Asn Ser Ser Lys Ser Val 210 215 220 Ile His Glu Asn Leu Asp Lys Asn Arg Glu Asp Ala Ile His Lys Ile 225 230 235 240 Leu Asn Arg Met Tyr Ala Val Val Met Lys Lys Ala Val Thr Glu Leu 245 250 255 Thr Lys Glu Asn Ile Ala Lys Tyr Arg Asp Ala Ile Asp Arg Met Lys 260 265 270 Gly Phe Lys Ser Ser Met Pro Gln Lys Lys 275 280 (2) INFORMATION FOR SEQ ID NO:82:

(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 280 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...280 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:82: Met Lys Leu Arg Ala Ser Val Leu Ile Gly Val Ala Ile Leu Cys Leu 1 5 10 15 Ile Leu Ser Ala Cys Ser Asn Tyr Ala Lys Lys Val Val Lys Gln Lys 20 25 30 Asn His Val Tyr Thr Pro Val Tyr Asn Glu Leu Ile Glu Lys Tyr Ser 35 40 45 Glu Ile Pro Leu Asn Asp Lys Leu Lys Asp Thr Pro Phe Met Val Gln 50 55 60 Val Lys Leu Pro Asn Tyr Lys Asp Tyr Leu Leu Asp Asn Lys Gln Val 65 70 75 80 Val Leu Thr Phe Lys Leu Val His His Ser Lys Lys Ile Thr Leu Ile 85 90 95 Gly Asp Ala Asn Lys Ile Leu Gln Tyr Lys Asn Tyr Phe Gln Ala Asn 100 105 110 Gly Ala Arg Ser Asp Ile Asp Phe Tyr Leu Gln Pro Thr Leu Asn Gln 115 120 125 Lys Gly Val Val Met Ile Ala Ser Asn Tyr Asn Asp Asn Pro Asn Asn 130 135 140 Lys Glu Lys Pro Gln Thr Phe Asp Val Leu Gln Gly Ser Gln Pro Met 145 150 155 160 Leu Gly Ala Asn Thr Lys Asn Leu His Gly Tyr Asp Val Ser Gly Ala 165 170 175 Asn Asn Lys Gln Val Ile Asn Glu Val Ala Arg Glu Lys Ala Gln Leu 180 185 190 Glu Lys Ile Asn Gln Tyr Tyr Lys Thr Leu Leu Gln Asp Lys Glu Gln 195 200 205 Glu Tyr Thr Thr Arg Lys Asn Asn Gln Arg Glu Ile Leu Glu Thr Leu 210 215 220 Ser Asn Arg Ala Gly Tyr Gln Met Arg Gln Asn Val Ile Ser Ser Glu 225 230 235 240 Ile Phe Lys Asn Gly Asn Leu Asn Met Gln Ala Lys Glu Glu Glu Val 245 250 255 Arg Glu Lys Leu Gln Glu Glu Arg Glu Asn Glu Tyr Leu Arg Asn Gln 260 265 270 Ile Arg Ser Leu Leu Ser Gly Lys 275 280

(2) INFORMATION FOR SEQ ID NO:83: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 393 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...393 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:83: Met Arg Lys Leu Phe Ile Pro Leu Leu Leu Phe Ser Ala Leu Glu Ala 1 5 10 15 Asn Glu Lys Asn Gly Phe Phe Ile Glu Ala Gly Phe Glu Thr Gly Leu 20 25 30 Leu Glu Gly Thr Gln Thr Gln Glu Lys Arg His Thr Thr Thr Lys Asn 35 40 45 Thr Tyr Ala Thr Tyr Asn Tyr Leu Pro Thr Asp Thr Ile Leu Lys Arg 50 55 60 Ala Ala Asn Leu Phe Thr Asn Ala Glu Ala Ile Ser Lys Leu Lys Phe 65 70 75 80 Ser Ser Leu Ser Pro Val Arg Val Leu Tyr Met Tyr Asn Gly Gln Leu 85 90 95 Thr Ile Glu Asn Phe Leu Pro Tyr Asn Leu Asn Asn Val Lys Leu Ser 100 105 110 Phe Thr Asp Ala Gln Gly Asn Val Ile Asp Leu Gly Val Ile Glu Thr 115 120 125 Ile Pro Lys His Ser Lys Ile Val Leu Pro Gly Glu Ala Phe Asp Ser 130 135 140 Leu Lys Ile Asp Pro Tyr Thr Leu Phe Leu Pro Lys Ile Glu Ala Thr 145 150 155 160 Ser Thr Ser Ile Ser Asp Ala Asn Thr Gln Arg Val Phe Glu Thr Leu 165 170 175 Asn Lys Ile Lys Thr Asn Leu Val Val Asn Tyr Arg Asn Glu Asn Lys 180 185 190 Phe Lys Asp His Glu Asn His Trp Glu Ala Phe Thr Pro Gln Thr Ala 195 200 205 Glu Glu Phe Thr Asn Leu Met Leu Asn Met Ile Ala Val Leu Asp Ser 210 215 220 Gln Ser Trp Gly Asp Ala Ile Leu Asn Ala Pro Phe Glu Phe Thr Asn 225 230 235 240 Ser Pro Thr Asp Cys Asp Asn Asp Pro Ser Lys Cys Val Asn Pro Gly 245 250 255 Thr Asn Gly Leu Val Asn Ser Lys Val Asp Gln Lys Tyr Val Leu Asn 260 265 270

Lys Gln Asp Ile Val Asn Lys Phe Lys Asn Lys Ala Asp Leu Asp Val 275 280 285 Ile Val Leu Lys Asp Ser Gly Val Val Gly Leu Gly Ser Asp Ile Thr 290 295 300 Pro Ser Asn Asn Asp Asp Gly Lys His Tyr Gly Gln Leu Gly Val Val 305 310 315 320 Ala Ser Ala Leu Asp Pro Lys Lys Leu Phe Gly Asp Asn Leu Lys Thr 325 330 335 Ile Asn Leu Glu Asp Leu Arg Thr Ile Leu His Glu Phe Ser His Thr 340 345 350 Lys Gly Tyr Gly His Asn Gly Asn Met Thr Tyr Gln Arg Val Pro Val 355 360 365 Thr Lys Asp Gly Gln Val Glu Lys Asp Ser Asn Gly Lys Pro Lys Asp 370 375 380 Ser Asp Gly Leu Pro Tyr Asn Val Cys 385 390 (2) INFORMATION FOR SEQ ID NO:84: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 270 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...270 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:84: Met Lys Lys Phe Val Ala Leu Gly Leu Leu Ser Ala Val Leu Ser Ser 1 5 10 15 Ser Leu Leu Ala Glu Gly Asp Gly Val Tyr Ile Gly Thr Asn Tyr Gln 20 25 30 Leu Gly Gln Ala Arg Leu Asn Ser Asn Ile Tyr Asn Thr Gly Asp Cys 35 40 45 Thr Gly Ser Val Val Gly Cys Pro Pro Gly Leu Thr Ala Asn Lys His 50 55 60 Asn Pro Gly Gly Thr Asn Ile Asn Trp His Ser Lys Tyr Ala Asn Gly 65 70 75 80 Ala Leu Asn Gly Phe Gly Leu Asn Val Gly Tyr Lys Lys Phe Phe Gln 85 90 95 Phe Lys Ser Leu Asp Met Thr Ser Lys Trp Phe Gly Phe Arg Val Tyr 100 105 110 Gly Leu Phe Asp Tyr Gly His Ala Asp Leu Gly Lys Gln Val Tyr Ala 115 120 125 Pro Asn Lys Ile Gln Leu Asp Met Val Ser Trp Gly Val Gly Ser Asp 130 135 140

Leu Leu Ala Asp Ile Ile Asp Lys Asp Asn Ala Ser Phe Gly Ile Phe 145 150 155 160 Gly Gly Val Ala Ile Gly Gly Asn Thr Trp Lys Ser Ser Ala Ala Asn 165 170 175 Tyr Trp Lys Glu Gln Ile Ile Glu Ala Lys Gly Pro Asp Val Cys Thr 180 185 190 Pro Thr Tyr Cys Asn Pro Asn Ala Pro Tyr Ser Thr Asn Thr Ser Thr 195 200 205 Val Ala Phe Gln Val Trp Leu Asn Phe Gly Val Arg Ala Asn Ile Tyr 210 215 220 Lys His Asn Gly Val Glu Phe Gly Val Arg Val Pro Leu Leu Ile Asn 225 230 235 240 Lys Phe Leu Ser Ala Gly Pro Asn Ala Thr Asn Leu Tyr Tyr His Leu 245 250 255 Lys Arg Asp Tyr Ser Leu Tyr Leu Gly Tyr Asn Tyr Thr Phe 260 265 270 (2) INFORMATION FOR SEQ ID NO:85: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 140 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...140 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:85: Met His Pro Ile Met Phe Ala Tyr Ile Ala Asn Ala Leu Ala Gln Ala 1 5 10 15 Arg Lys Ile Asn Gly Thr Leu Cys Met Ala Phe Gln Lys Ile Ser Gln 20 25 30 Val Lys Glu Leu Gly Ile Asp Lys Ala Lys Ser Leu Ile Gly Asn Leu 35 40 45 Ser Gln Val Ile Ile Tyr Pro Thr Lys Asp Thr Asp Glu Leu Ile Glu 50 55 60 Cys Gly Val Pro Leu Ser Asp Ser Glu Ile Asn Phe Leu His Asn Thr 65 70 75 80 Asp Met Arg Ala Arg Gln Val Leu Val Lys Asn Ile Val Thr Asn Ala 85 90 95 Ser Ala Phe Ile Glu Ile Asp Leu Lys Lys Ile Cys Lys Asn Tyr Phe 100 105 110 Ile Phe Leu Ile Ala Met Leu Val Ile Glu Lys Ser Ser Met Ile Leu 115 120 125 Lys Lys Gln Thr Lys Lys Leu Ile Arg Lys Ser Ile 130 135 140

(2) INFORMATION FOR SEQ ID NO:86: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 256 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...256 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:86: Met Leu Gly Ser Val Lys Lys Ala Val Phe Arg Val Leu Cys Leu Gly 1 5 10 15 Ala Leu Cys Leu Cys Gly Gly Leu Met Ala Glu Gln Asp Pro Lys Glu 20 25 30 Leu Ile Phe Ser Gly Ile Thr Ile Tyr Thr Asp Lys Asn Phe Thr Arg 35 40 45 Ala Lys Lys Tyr Phe Glu Lys Ala Cys Lys Ser Asn Asp Ala Asp Gly 50 55 60 Cys Ala Ile Leu Arg Glu Val Tyr Ser Ser Gly Lys Ala Ile Ala Arg 65 70 75 80 Glu Asn Ala Arg Glu Ser Ile Glu Lys Ala Leu Glu His Thr Ala Thr 85 90 95 Ala Lys Val Cys Lys Leu Asn Asp Ala Glu Lys Cys Lys Asp Leu Ala 100 105 110 Glu Phe Tyr Phe Asn Val Asn Asp Leu Lys Asn Ala Leu Glu Tyr Tyr 115 120 125 Ser Lys Ser Cys Lys Leu Asn Asn Val Glu Gly Cys Met Leu Ser Ala 130 135 140 Thr Phe Tyr Asn Asp Met Ile Lys Gly Leu Lys Lys Asp Lys Lys Asp 145 150 155 160 Leu Glu Tyr Tyr Ser Lys Ala Cys Glu Leu Asn Asn Gly Gly Gly Cys 165 170 175 Ser Lys Leu Gly Gly Asp Tyr Phe Phe Gly Glu Gly Val Thr Lys Asp 180 185 190 Phe Lys Lys Ala Phe Glu Tyr Ser Ala Lys Ala Cys Glu Leu Asn Asp 195 200 205 Ala Lys Gly Cys Tyr Ala Leu Ala Ala Phe Tyr Asn Glu Gly Lys Gly 210 215 220 Val Ala Lys Asp Glu Lys Gln Thr Thr Glu Asn Leu Glu Lys Ser Cys 225 230 235 240 Lys Leu Gly Leu Lys Glu Ala Cys Asp Ile Leu Lys Glu Gln Lys Gln 245 250 255 (2) INFORMATION FOR SEQ ID NO:87:

(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 242 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...242 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:87: Met Lys Lys Phe Phe Ser Gln Ser Leu Leu Ala Leu Ile Ile Ser Met 1 5 10 15 Asn Ala Val Ser Gly Met Asp Gly Asn Gly Val Phe Leu Gly Ala Gly 20 25 30 Tyr Leu Gln Gly Gln Ala Gln Met His Ala Asp Ile Asn Ser Gln Lys 35 40 45 Gln Ala Thr Asn Ala Thr Ile Lys Gly Phe Asp Ala Leu Leu Gly Tyr 50 55 60 Gln Phe Phe Phe Glu Lys His Phe Gly Leu Arg Leu Tyr Gly Phe Phe 65 70 75 80 Asp Tyr Ala His Ala Asn Ser Ile Lys Leu Lys Asn Pro Asn Tyr Asn 85 90 95 Ser Glu Ala Ala Gln Val Ala Ser Gln Ile Leu Gly Lys Gln Glu Ile 100 105 110 Asn Arg Leu Thr Asn Ile Ala Asp Pro Arg Thr Phe Glu Pro Asn Met 115 120 125 Leu Thr Tyr Gly Gly Ala Met Asp Val Met Val Asn Val Ile Asn Asn 1,30 135 140 Gly Ile Met Ser Leu Gly Ala Phe Gly Gly Ile Gln Leu Ala Gly Asn 145 150 155 160 Ser Trp Leu Met Ala Thr Pro Ser Phe Glu Gly Ile Leu Val Glu Gln 165 170 175 Ala Leu Val Ser Lys Lys Ala Thr Ser Phe Gln Phe Leu Phe Asn Val 180 185 190 Gly Ala Arg Leu Arg Ile Leu Lys His Ser Ser Ile Glu Ala Gly Val 195 200 205 Lys Phe Pro Met Leu Lys Lys Asn Pro Tyr Ile Thr Ala Lys Asn Leu 210 215 220 Asp Ile Gly Phe Arg Arg Val Tyr Ser Trp Tyr Val Asn Tyr Val Phe 225 230 235 240 Thr Phe (2) INFORMATION FOR SEQ ID NO:88: (i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 267 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...267 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:88: Met Asn Tyr Pro Asn Leu Pro Asn Ser Ala Leu Glu Ile Ser Glu Gln 1 5 10 15 Pro Glu Val Lys Glu Ile Thr Asn Glu Leu Leu Lys Gln Leu Gln Asn 20 25 30 Ala Leu Arg Ser Asn Ala His Phe Ser Glu Gln Val Glu Leu Ser Leu 35 40 45 Lys Cys Ile Val Arg Ile Leu Glu Val Leu Leu Ser Leu Asp Phe Phe 50 55 60 Lys Asn Ala Asn Glu Ile Asp Ser Ser Leu Arg Asn Ser Ile Glu Trp 65 70 75 80 Leu Thr Asn Ala Gly Glu Ser Leu Lys Leu Lys Met Lys Glu Tyr Glu 85 90 95 Arg Phe Phe Ser Glu Phe Asn Thr Ser Met His Ala Asn Glu Gln Glu 100 105 110 Val Thr Asn Thr Leu Asn Ala Asn Ala Glu Asn Ile Lys Ser Glu Ile 115 120 125 Lys Lys Leu Glu Asn Gln Leu Ile Glu Thr Thr Thr Arg Leu Leu Thr 130 135 140 Ser Tyr Gln Ile Phe Leu Asn Gln Ala Arg Asp Asn Ala Asn Asn Gln 145 150 155 160 Ile Thr Lys Asn Lys Thr Gln Ser Leu Glu Ala Ile Thr Gln Ala Lys 165 170 175 Asn Asn Ala Asn Asn Glu Ile Ser Asn Asn Gln Thr Gln Ala Ile Thr 180 185 190 Asn Ile Thr Glu Ala Lys Thr Asn Ala Asn Asn Glu Ile Ser Asn Asn 195 200 205 Gln Thr Gln Ala Ile Thr Asn Ile Asn Glu Ala Lys Glu Ser Ala Thr 210 215 220 Thr Gln Ile Asn Ala Asn Lys Gln Glu Ala Ile Asn Asn Ile Thr Gln 225 230 235 240 Glu Lys Thr Gln Ala Thr Ser Glu Ile Thr Glu Ala Lys Lys Thr Asp 245 250 255 His Tyr Gln Asn Ile Asp Phe Phe Glu Phe Glu 260 265 (2) INFORMATION FOR SEQ ID NO:89: (i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 544 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...544 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:89: Val Ile Glu Thr Ile Pro Lys His Ser Lys Ile Val Leu Pro Gly Glu 1 5 10 15 Ala Phe Asp Ser Leu Lys Glu Ala Phe Asp Lys Ile Asp Pro Tyr Thr 20 25 30 Phe Phe Phe Pro Lys Phe Glu Ala Thr Ser Thr Ser Ile Ser Asp Thr 35 40 45 Asn Thr Gln Arg Val Phe Glu Thr Leu Asn Asn Ile Lys Thr Asn Leu 50 55 60 Ile Met Lys Tyr Ser Asn Glu Asn Pro Asn Asn Phe Asn Thr Cys Pro 65 70 75 80 Tyr Asn Asn Asn Gly Asn Thr Lys Asn Asp Cys Trp Gln Asn Phe Thr 85 90 95 Pro Gln Thr Ala Glu Glu Phe Thr Asn Leu Met Leu Asn Met Ile Ala 100 105 110 Val Leu Asp Ser Gln Ser Trp Gly Asp Ala Ile Leu Asn Ala Pro Phe 115 120 125 Glu Phe Thr Asn Ser Ser Thr Asp Cys Asp Ser Asp Pro Ser Lys Cys 130 135 140 Val Asn Pro Gly Val Asn Gly Arg Val Asp Thr Lys Val Asp Gln Gln 145 150 155 160 Tyr Ile Leu Asn Lys Gln Gly Ile Ile Asn Asn Phe Arg Lys Lys Ile 165 170 175 Glu Ile Asp Ala Val Val Leu Lys Asn Ser Gly Val Val Gly Leu Ala 180 185 190 Asn Gly Tyr Gly Asn Asp Gly Glu Tyr Gly Thr Leu Gly Val Glu Ala 195 200 205 Tyr Ala Leu Asp Pro Lys Lys Leu Phe Gly Asn Asp Leu Lys Thr Ile 210 215 220 Asn Leu Glu Asp Leu Arg Thr Ile Leu His Glu Phe Ser His Thr Lys 225 230 235 240 Gly Tyr Gly His Asn Gly Asn Met Thr Tyr Gln Arg Val Pro Val Thr 245 250 255 Lys Asp Gly Gln Val Glu Lys Asp Ser Asn Gly Lys Pro Lys Asp Ser 260 265 270 Asp Gly Leu Pro Tyr Asn Val Cys Ser Leu Tyr Gly Gly Ser Asn Gln 275 280 285 Pro Ala Phe Pro Ser Asn Tyr Pro Asn Ser Ile Tyr His Asn Cys Ala 290 295 300

Asp Val Pro Ala Gly Phe Leu Gly Val Thr Ala Ala Val Trp Gln Gln 305 310 315 320 Leu Ile Asn Gln Asn Ala Leu Pro Ile Asn Tyr Ala Asn Leu Gly Ser 325 330 335 Gln Thr Asn Tyr Asn Leu Asn Ala Ser Leu Asn Thr Gln Asp Leu Ala 340 345 350 Asn Ser Met Leu Ser Thr Ile Gln Lys Thr Phe Val Thr Ser Ser Val 355 360 365 Thr Asn His His Phe Ser Asn Ala Ser Gln Ser Phe Arg Ser Pro Ile 370 375 380 Leu Gly Val Asn Ala Lys Ile Gly Tyr Gln Asn Tyr Phe Asn Asp Phe 385 390 395 400 Ile Gly Leu Ala Tyr Tyr Gly Ile Ile Lys Tyr Asn Tyr Ala Lys Ala 405 410 415 Val Asn Gln Lys Val Gln Gln Leu Ser Tyr Gly Gly Gly Ile Asp Leu 420 425 430 Leu Leu Asp Phe Ile Thr Thr Tyr Ser Asn Lys Asn Ser Pro Thr Gly 435 440 445 Ile Gln Thr Lys Arg Asn Phe Ser Ser Ser Phe Gly Ile Phe Gly Gly 450 455 460 Leu Arg Gly Leu Tyr Asn Ser Tyr Tyr Val Leu Asn Lys Val Lys Gly 465 470 475 480 Ser Gly Asn Leu Asp Val Ala Thr Gly Leu Asn Tyr Arg Tyr Lys His 485 490 495 Ser Lys Tyr Ser Val Gly Ile Ser Ile Pro Leu Ile Gln Arg Lys Ala 500 505 510 Ser Val Val Ser Ser Gly Gly Asp Tyr Thr Asn Ser Phe Val Phe Asn 515 520 525 Glu Gly Ala Ser His Phe Lys Val Phe Phe Asn Tyr Gly Gly Cys Phe 530 535 540 (2) INFORMATION FOR SEQ ID NO:90: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 356 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...356 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:90: Leu Met Lys Ser Ile Leu Leu Phe Met Ile Phe Val Val Cys Gln Leu 1 5 10 15 Glu Gly Lys Lys Phe Ser Gln Asp Asn Phe Lys Val Asp Tyr Asn Tyr 20 25 30

Tyr Leu Arg Lys Gln Asp Leu His Ile Ile Lys Thr Gln Asn Asp Leu 35 40 45 Ser Asn Ala Trp Tyr Leu Pro Pro Gln Lys Ala Pro Lys Glu His Ser 50 55 60 Trp Val Asp Phe Ala Lys Lys Tyr Leu Asn Met Met Asp Tyr Leu Gly 65 70 75 80 Thr Tyr Phe Leu Pro Phe Tyr His Ser Phe Thr Pro Ile Phe Gln Trp 85 90 95 Tyr His Pro Asn Ile Asn Pro Tyr Gln Arg Asn Glu Phe Lys Phe Gln 100 105 110 Ile Ser Phe Arg Val Pro Val Phe Arg His Ile Leu Trp Thr Lys Gly 115 120 125 Thr Leu Tyr Leu Ala Tyr Thr Gln Thr Asn Trp Phe Gln Ile Tyr Asn 130 135 140 Asp Pro Gln Ser Ala Pro Met Arg Met Ile Asn Phe Met Pro Glu Leu 145 150 155 160 Ile Tyr Val Tyr Pro Ile Asn Phe Lys Pro Phe Gly Gly Lys Ile Gly 165 170 175 Asn Phe Ser Glu Ile Trp Ile Gly Trp Gln His Ile Ser Asn Gly Val 180 185 190 Gly Gly Ala Gln Cys Tyr Gln Pro Phe Asn Lys Glu Gly Asn Pro Glu 195 200 205 Asn Gln Phe Pro Gly Gln Pro Val Ile Val Lys Asp Tyr Asn Gly Gln 210 215 220 Lys Asp Val Arg Trp Gly Gly Cys Xaa Ser Val Xaa Xaa Gly Asn Xaa 225 230 235 240 Leu Cys Phe Val Leu Val Trp Glu Lys Gly Gly Leu Lys Ile Met Val 245 250 255 Ala Tyr Trp Pro Tyr Val Pro Tyr Asp Gln Ser Asn Pro Gln Leu Ile 260 265 270 Asp Tyr Met Gly Tyr Gly Asn Ala Lys Ile Asp Tyr Arg Arg Gly Arg 275 280 285 His His Phe Glu Leu Gln Leu Tyr Asp Ile Phe Thr Gln Tyr Trp Arg 290 295 300 Tyr Asp Arg Trp His Gly Ala Phe Arg Leu Gly Tyr Thr Tyr Arg Ile 305 310 315 320 Asn Pro Phe Val Gly Ile Tyr Ala Gln Trp Phe Asn Gly Tyr Gly Asp 325 330 335 Gly Leu Tyr Glu Tyr Asp Val Phe Ser Asn Arg Ile Gly Val Gly Ile 340 345 350 Arg Leu Asn Pro 355 (2) INFORMATION FOR SEQ ID NO:91: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 675 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE:

(A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...675 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:91: Leu Ser Lys Gly Leu Ser Ile Gly Asn Lys Ile Ile Leu Cys Val Ala 1 5 10 15 Leu Ile Val Ile Val Cys Val Ser Ile Leu Gly Val Ser Leu Asn Ser 20 25 30 Arg Val Lys Glu Ile Leu Lys Glu Ser Ala Leu His Ser Met Gln Asp 35 40 45 Ser Leu His Phe Lys Val Lys Glu Val Gln Ser Val Leu Glu Asn Thr 50 55 60 Tyr Thr Ser Met Gly Ile Val Lys Glu Met Leu Pro Glu Asp Thr Lys 65 70 75 80 Arg Glu Ile Lys Ile Gln Leu Leu Lys Asn Phe Ile Leu Ala Asn Ser 85 90 95 His Val Ala Gly Val Ser Met Phe Phe Lys Asp Arg Glu Asp Leu Arg 100 105 110 Leu Thr Leu Leu Arg Asp Asn Asp Thr Ile Lys Leu Met Glu Asn Pro 115 120 125 Ser Leu Gly Ser Asn Pro Leu Ala Gln Lys Ala Met Lys Asn Lys Glu 130 135 140 Ile Ser Lys Ser Leu Pro Tyr Tyr Arg Lys Met Pro Asn Gly Ala Glu 145 150 155 160 Val Tyr Gly Val Asp Ile Leu Leu Pro Leu Phe Lys Glu Asn Thr Gln 165 170 175 Glu Val Val Gly Val Leu Met Ile Phe Phe Ser Ile Asp Ser Phe Ser 180 185 190 Asn Glu Ile Thr Lys Asn Arg Ser Asp Leu Phe Leu Ile Gly Val Lys 195 200 205 Gly Lys Val Leu Leu Ser Ala Asn Lys Ser Leu Gln Asp Lys Ser Ile 210 215 220 Thr Glu Ile Tyr Lys Ser Val Pro Lys Ala Thr Asn Glu Val Met Ala 225 230 235 240 Ile Leu Glu Asn Gly Ser Lys Ala Thr Leu Glu Tyr Leu Asp Pro Phe 245 250 255 Ser His Lys Glu Asn Phe Leu Ala Val Glu Thr Phe Lys Met Leu Gly 260 265 270 Lys Thr Glu Ser Lys Asp Asn Leu Asn Trp Met Ile Ala Leu Ile Ile 275 280 285 Glu Lys Asp Lys Val Tyr Glu Gln Val Gly Ser Val Arg Phe Val Val 290 295 300 Val Ala Ala Ser Ala Ile Met Val Leu Ala Leu Ile Ile Ala Ile Thr 305 310 315 320 Leu Leu Met Arg Ala Ile Val Ser Asn Arg Leu Glu Val Val Ser Ser 325 330 335 Thr Leu Ser His Phe Phe Lys Leu Leu Asn Asn Gln Ala His Ser Ser 340 345 350 Asp Ile Lys Leu Val Glu Ala Arg Ser Asn Asp Glu Leu Gly Arg Met 355 360 365 Gln Thr Ala Ile Asn Lys Asn Ile Leu Gln Thr Gln Lys Thr Met Gln

370 375 380 Glu Asp Arg Gln Ala Val Gln Asp Thr Ile Lys Val Val Ser Asp Val 385 390 395 400 Lys Ala Gly Asn Phe Ala Val Arg Ile Thr Ala Glu Pro Ala Ser Pro 405 410 415 Asp Leu Lys Glu Leu Arg Asp Ala Leu Asn Gly Ile Met Asp Tyr Leu 420 425 430 Gln Glu Ser Val Gly Thr His Met Pro Ser Ile Phe Lys Ile Phe Glu 435 440 445 Ser Tyr Ser Gly Leu Asp Phe Arg Gly Arg Ile Gln Asn Ala Ser Gly 450 455 460 Arg Val Glu Leu Val Thr Asn Ala Leu Gly Gln Glu Ile Gln Lys Met 465 470 475 480 Leu Glu Thr Ser Ser Asn Phe Ala Lys Asp Leu Ala Asn Asp Ser Ala 485 490 495 Asn Leu Lys Glu Cys Val Gln Asn Leu Glu Lys Ala Ser Asn Ser Gln 500 505 510 His Lys Ser Leu Met Glu Thr Ser Lys Thr Ile Glu Asn Ile Thr Thr 515 520 525 Ser Ile Gln Gly Val Ser Ser Gln Ser Glu Ala Met Ile Glu Gln Gly 530 535 540 Lys Asp Ile Lys Ser Ile Val Glu Ile Ile Arg Asp Ile Ala Asp Gln 545 550 555 560 Thr Asn Leu Leu Ala Leu Asn Ala Ala Ile Glu Ala Ala Arg Ala Gly 565 570 575 Glu His Gly Arg Gly Phe Ala Val Val Ala Asp Glu Val Arg Lys Leu 580 585 590 Ala Glu Arg Thr Gln Lys Ser Leu Ser Glu Ile Glu Ala Asn Ile Asn 595 600 605 Ile Leu Val Gln Ser Ile Ser Asp Thr Ser Glu Ser Ile Lys Asn Gln 610 615 620 Val Lys Glu Val Glu Glu Ile Asn Ala Ser Ile Glu Ala Leu Arg Ser 625 630 635 640 Val Thr Glu Gly Asn Leu Lys Ile Ala Ser Asp Ser Leu Glu Ile Ser 645 650 655 Gln Glu Ile Asp Lys Val Ser Asn Asp Ile Leu Glu Asp Val Asn Lys 660 665 670 Lys Gln Phe 675 (2) INFORMATION FOR SEQ ID NO:92: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 271 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE:

(A) NAME/KEY: misc feature (B) LOCATION 1...271 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:92: Met Asn Ile Phe Lys Arg Ile Ile Cys Val Thr Ala Ile Val Leu Gly 1 5 10 15 Phe Phe Asn Leu Leu Asp Ala Lys His His Lys Glu Lys Lys Glu Asp 20 25 30 His Lys Ile Thr Arg Glu Leu Lys Val Gly Ala Asn Pro Val Pro His 35 40 45 Ala Gln Ile Leu Gln Ser Val Val Asp Asp Leu Lys Glu Lys Gly Ile 50 55 60 Lys Leu Val Ile Val Ser Phe Thr Asp Tyr Val Leu Pro Asn Leu Ala 65 70 75 80 Leu Asn Asp Gly Ser Leu Asp Ala Asn Tyr Phe Gln His Arg Pro Tyr 85 90 95 Leu Asp Arg Phe Asn Leu Asp Arg Lys Met His Leu Val Gly Leu Ala 100 105 110 Asn Ile His Val Glu Pro Leu Arg Phe Tyr Ser Gln Lys Ile Thr Asp 115 120 125 Ile Lys Asn Leu Lys Lys Gly Ser Val Ile Ala Val Pro Asn Asp Pro 130 135 140 Ala Asn Gln Gly Arg Ala Leu Ile Leu Leu His Lys Gln Gly Leu Ile 145 150 155 160 Ala Leu Lys Asp Pro Ser Asn Leu Tyr Ala Thr Glu Phe Asp Ile Val 165 170 175 Lys Asn Pro Tyr Asn Ile Lys Ile Lys Pro Leu Glu Ala Ala Leu Leu 180 185 190 Pro Lys Val Leu Gly Asp Val Asp Gly Ala Ile Ile Thr Gly Asn Tyr 195 200 205 Ala Leu Gln Ala Lys Leu Thr Gly Ala Leu Phe Ser Glu Asp Lys Asp 210 215 220 Ser Pro Tyr Ala Asn Leu Val Ala Ser Arg Glu Asp Asn Ala Gln Asp 225 230 235 240 Glu Ala Ile Lys Ala Leu Ile Glu Ala Leu Gln Ser Glu Lys Thr Arg 245 250 255 Lys Phe Ile Leu Asp Thr Tyr Lys Gly Ala Ile Ile Pro Ala Phe 260 265 270 (2) INFORMATION FOR SEQ ID NO:93: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 161 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE:

(A) NAME/KEY: misc~feature (B) LOCATION 1...161 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:93: Met Phe Phe Lys Thr Tyr Gln Lys Leu Leu Gly Ala Ser Cys Leu Ala 1 5 10 15 Leu Tyr Leu Val Gly Cys Gly Asn Gly Gly Gly Gly Glu Ser Pro Val 20 25 30 Glu Met Ile Ala Asn Ser Glu Gly Thr Phe Gln Ile Asp Ser Lys Ala 35 40 45 Asp Ser Ile Thr Ile Gln Gly Val Lys Leu Asn Arg Gly Asn Cys Ala 50 55 60 Val Asn Phe Val Pro Val Ser Glu Thr Phe Gln Met Gly Val Leu Ser 65 70 75 80 Gln Val Thr Pro Ile Ser Ile Gln Asp Phe Lys Asp Met Ala Ser Thr 85 90 95 Tyr Lys Ile Phe Asp Gln Lys Lys Gly Leu Ala Asn Ile Ala Asn Lys 100 105 110 Ile Ser Gln Leu Glu Gln Lys Gly Val Met Met Glu Pro Gln Thr Leu 115 120 125 Asn Phe Gly Glu Ser Leu Lys Gly Ile Ser Gln Gly Cys Asn Ile Ile 130 135 140 Glu Ala Glu Ile Gln Thr Asp Lys Gly Ala Trp Thr Phe Asn Phe Asp 145 150 155 160 Lys (2) INFORMATION FOR SEQ ID NO:94: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 337 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1... .337 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:94: Met Ile Arg Leu Lys Gly Leu Asn Lys Thr Leu Lys Thr Ser Leu Leu 1 5 10 15 Ala Gly Val Leu Leu Gly Ala Thr Ala Pro Leu Met Ala Lys Pro Leu 20 25 30 Leu Ser Asp Glu Asp Leu Leu Lys Arg Val Lys Leu His Asn Ile Lys 35 40 45 Glu Asp Thr Leu Thr Ser Cys Asn Ala Lys Val Asp Gly Ser Gln Tyr

50 55 60 Leu Asn Ser Gly Trp Asn Leu Ser Lys Glu Phe Pro Gln Glu Tyr Arg 65 70 75 80 Glu Lys Ile Phe Glu Cys Val Glu Glu Glu Lys His Lys Gln Ala Leu 85 90 95 Asn Leu Ile Asn Lys Glu Asp Thr Glu Asp Lys Glu Glu Leu Ala Lys 100 105 110 Lys Ile Lys Glu Ile Lys Glu Lys Ala Lys Val Leu Arg Gln Lys Phe 115 120 125 Met Ala Phe Glu Met Lys Glu His Ser Lys Glu Phe Pro Asn Lys Lys 130 135 140 Gln Leu Gln Thr Met Leu Glu Asn Ala Phe Asp Asn Gly Ala Glu Ser 145 150 155 160 Phe Ile Asp Asp Trp His Glu Arg Phe Gly Gly Ile Ser Arg Glu Asn 165 170 175 Thr Tyr Lys Ala Leu Gly Ile Lys Glu Tyr Ser Asp Glu Gly Lys Ile 180 185 190 Leu Ala Phe Gly Glu Arg Ser Tyr Ile Arg Gln Tyr Lys Lys Asp Phe 195 200 205 Glu Glu Ser Thr Tyr Asp Thr Arg Gln Thr Leu Ser Ala Met Ala Asn 210 215 220 Met Ser Gly Glu Asn Asp Tyr Lys Ile Thr Trp Leu Lys Pro Lys Tyr 225 230 235 240 Gln Leu His Ser Ser Asn Asn Ile Lys Pro Leu Met Ser Asn Thr Glu 245 250 255 Leu Leu Asn Met Ile Glu Leu Thr Asn Ile Lys Lys Glu Tyr Val Met 260 265 270 Gly Cys Asn Met Glu Ile Asp Gly Ser Lys Tyr Pro Ile His Lys Asp 275 280 285 Trp Gly Phe Phe Gly Lys Ala Lys Val Pro Glu Thr Trp Arg Asn Lys 290 295 300 Ile Trp Glu Cys Ile Lys Asn Lys Val Lys Ser Tyr Asp Asn Thr Thr 305 310 315 320 Ala Glu Ile Gly Ile Val Trp Lys Lys Asn Thr Tyr Ser Ile Ser His 325 330 335 His (2) INFORMATION FOR SEQ ID NO:95: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 416 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...416

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:95: Met Lys Lys Leu Val Phe Ser Met Leu Leu Cys Cys Lys Ser Val Phe 1 5 10 15 Ala Glu Gly Glu Thr Pro Leu Ile Val Asn Asp Pro Glu Thr His Val 20 25 30 Ser Gln Ala Thr Ile Ile Gly Lys Met Val Asp Ser Ile Lys Arg Tyr 35 40 45 Glu Glu Ile Ile Ser Lys Ala Gln Ala Gln Val Asn Gln Leu Gln Lys 50 55 60 Val Asn Asn Met Ile Asn Thr Thr Asn Ser Leu Ile Ser Ser Ser Ala 65 70 75 80 Ile Thr Leu Ala Asn Pro Met Gln Val Leu Gln Asn Ala Gln Tyr Gln 85 90 95 Ile Glu Ser Ile Arg Tyr Asn Tyr Glu Asn Leu Lys Gln Ser Ile Glu 100 105 110 Asn Trp Asn Ala Gln Asn Leu Leu Arg Asn Lys Tyr Leu Gln Gln Gln 115 120 125 Cys Pro Trp Leu Asn Val Asn Ala Leu Thr Asn Asn Lys Ile Val Asn 130 135 140 Leu Lys Asp Leu Asn Asn Leu Ile Thr Lys Asn Gly Glu Gln Thr Gln 145 150 155 160 Thr Ala Arg Asp Val Gln Asn Leu Ile Gln Ser Ile Ser Gly Ser Gly 165 170 175 Tyr Gly Asn Met Gln Ser Leu Ala Gly Glu Leu Ser Gly Arg Ala Trp 180 185 190 Gly Glu Met Leu Cys Lys Met Val Asn Asp Ser Asn Tyr Glu Ser Glu 195 200 205 Gln Ala Leu Leu Ala Thr Gly Asn Asn Pro Glu Glu Gln Lys Arg Arg 210 215 220 Phe Leu Leu Arg Val Lys Lys Lys Val Asn Asp Asn Lys Gln Leu Lys 225 230 235 240 Asp Lys Leu Asp Pro Phe Leu Lys Arg Leu Asp Val Leu Gln Thr Glu 245 250 255 Phe Gly Val Thr Asp Pro Thr Ala Asn His Asn Lys Gln Gly Ile His 260 265 270 Tyr Cys Thr Glu Asn Lys Glu Thr Gly Lys Cys Asp Pro Ile Lys Asn 275 280 285 Val Phe Arg Thr Thr Arg Leu Asp Asn Glu Leu Glu Gln Glu Ile Gln 290 295 300 Thr Leu Thr Leu Asp Leu Ile Lys Ala Ser Asn Lys Asp Ala Gln Ser 305 310 315 320 Gln Ala Tyr Ala Asn Phe Asn Gln Arg Ile Lys Leu Leu Thr Leu Lys 325 330 335 Tyr Leu Lys Glu Ile Thr Asn Gln Met Leu Phe Leu Asn Gln Thr Met 340 345 350 Ala Met Gln Ser Glu Ile Met Thr Asp Asp Tyr Phe Arg Gln Asn Asn 355 360 365 Asp Gly Phe Gly Glu Lys Glu Asn His Ile Asp Lys Gln Leu Thr Gln 370 375 380 Lys Arg Ile Asn Glu Arg Glu Arg Ala Arg Ile Tyr Phe Gln Asn Pro 385 390 395 400 Asn Val Lys Phe Asp Gln Phe Gly Phe Pro Ile Phe Ser Ile Trp Asp 405 410 415

(2) INFORMATION FOR SEQ ID NO:96: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 376 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...376 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:96: Val Asn Lys Trp Ile Lys Gly Ala Val Val Phe Val Gly Gly Phe Ala 1 5 10 15 Thr Ile Thr Thr Phe Ser Leu Ile Tyr His Gln Lys Pro Lys Ala Pro 20 25 30 Leu Asn Asn Gln Pro Ser Leu Leu Asn Asp Asp Glu Val Lys Tyr Pro 35 40 45 Leu Gln Asp Tyr Thr Phe Thr Gln Asn Pro Gln Pro Thr Asn Thr Glu 50 55 60 Ser Ser Lys Asp Ala Thr Ile Lys Ala Leu Gln Glu Gln Leu Lys Ala 65 70 75 80 Ala Leu Lys Ala Leu Asn Ser Lys Glu Met Asn Tyr Ser Lys Glu Glu 85 90 95 Thr Phe Thr Ser Pro Pro Met Asp Pro Lys Thr Thr Pro Pro Lys Lys 100 105 110 Asp Phe Ser Pro Lys Gln Leu Asp Leu Leu Ala Ser Arg Ile Thr Pro 115 120 125 Phe Lys Gln Ser Pro Lys Asn Tyr Glu Glu Asn Leu Ile Phe Pro Val 130 135 140 Asp Asn Pro Asn Gly Ile Asp Ser Phe Thr Asn Leu Lys Glu Lys Asp 145 150 155 160 Ile Ala Thr Asn Glu Asn Lys Leu Leu Arg Thr Ile Thr Ala Asp Lys 165 170 175 Met Ile Pro Ala Phe Leu Ile Thr Pro Ile Ser Ser Gln Ile Ala Gly 180 185 190 Lys Val Ile Ala Gln Val Glu Ser Asp Ile Phe Ala Ser Met Gly Lys 195 200 205 Ala Val Leu Ile Pro Lys Gly Ser Lys Val Ile Gly Tyr Tyr Ser Asn 210 215 220 Asn Asn Lys Met Gly Glu Tyr Arg Leu Asp Ile Val Trp Ser Arg Ile 225 230 235 240 Ile Thr Pro His Gly Ile Asn Ile Met Leu Thr Asn Ala Lys Gly Ala 245 250 255 Asp Ile Lys Gly Tyr Asn Gly Leu Val Gly Glu Leu Ile Glu Arg Asn 260 265 270

Phe Gln Arg Tyr Gly Val Pro Leu Leu Leu Ser Thr Leu Thr Asn Gly 275 280 285 Leu Leu Ile Gly Ile Thr Ser Ala Leu Asn Asn Arg Gly Asn Lys Glu 290 295 300 Glu Val Thr Asn Phe Phe Gly Asp Tyr Leu Leu Leu Gln Leu Met Arg 305 310 315 320 Gln Ser Gly Met Gly Ile Asn Gln Val Val Asn Gln Ile Leu Arg Asp 325 330 335 Lys Ser Lys Ile Ala Pro Ile Val Val Ile Arg Glu Gly Ser Arg Val 340 345 350 Phe Ile Ser Pro Asn Thr Asp Ile Phe Phe Pro Ile Pro Arg Glu Asn 355 360 365 Glu Val Ile Ala Glu Phe Leu Lys 370 375 (2) INFORMATION FOR SEQ ID NO:97: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 916 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...916 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:97: Val Asp Leu Arg Ile Gln Ser Lys Glu Val Ser His Asn Leu Lys Glu 1 5 10 15 Leu Ser Lys Thr Leu Ile Ser Tyr Pro Phe Glu Lys His Val Glu Ala 20 25 30 Leu Gly Glu Gln Cys Ser Asn Phe Val Ser Ile Pro Ile Asn Asn Asp 35 40 45 Asp Tyr Ser Asn Ile Cys Thr Phe Val Ser Asp Phe Ile Asn Leu Ile 50 55 60 Ala Ser Tyr Asn Leu Leu Glu Ser Phe Leu Asp Phe Tyr Lys Asp Lys 65 70 75 80 Leu Lys Leu Ser Glu Leu Val Thr Glu Tyr Ala Asn Val Thr Asn Asn 85 90 95 Leu Leu Phe Lys Lys Leu Ile Lys His Leu Ser Gly Asn Asn Gln Leu 100 105 110 Val Lys Asn Phe Tyr Gln Cys Ile Arg Glu Ile Ile Lys Tyr Asn Ala 115 120 125 Pro Asn Lys Glu Tyr Lys Pro Asn Gln Phe Phe Ile Ile Gly Lys Gly 130 135 140 Lys Gln Lys Gln Leu Ala Lys Ile Tyr Ser His Leu Lys Glu Leu Ser 145 150 155 160

Ala Ser Glu Ile Lys Pro Gln Asp Met Glu Asp Ile Leu Lys Lys Leu 165 170 175 Glu Glu Leu Asp Lys Ile Phe Lys Thr Thr Asp Phe Thr Lys Phe Thr 180 185 190 Pro Lys Thr Glu Ile Lys Asp Ile Ile Lys Glu Ile Asp Glu Lys Tyr 195 200 205 Pro Ile Asn Glu Asn Phe Lys Arg Gln Phe Asn Glu Phe Glu Ser Asn 210 215 220 Ile Glu Lys His Asp Glu Ile Lys Lys Asp Phe Glu Arg Asn Lys Glu 225 230 235 240 Ser Leu Ile Arg Glu Ile Glu Asn His Cys Lys Asn Glu Cys Asn Ser 245 250 255 Glu Glu Glu Pro Glu Tyr Lys Ile Asn Asp Leu Leu Lys Asn Ile Gln 260 265 270 Gln Ile Cys Lys Asn Tyr Ile Glu Ser His Ala Val Asn Asp Val Ser 275 280 285 Lys Asp Ile Lys Ser Met Met Cys Gln Phe Tyr Leu Lys Gln Ile Asp 290 295 300 Leu Leu Val Asn Ser Glu Ile Val Arg Tyr Arg Tyr Ser Asn Leu Phe 305 310 315 320 Glu Pro Ile Gln Arg Ser Leu Trp Glu Ser lie Lys Ile Leu Asp Asn 325 330 335 Glu Ser Gly Ile Tyr Leu Phe Pro Lys Asn Ile Gly Glu Ile Lys Asp 340 345 350 Lys Phe Glu Ala Asn Lys Glu Lys Phe Lys Gln Ser Lys Asn Val Ser 355 360 365 Glu Phe Ala Glu Tyr Cys Arg Glu Cys Asn Pro Tyr Thr Ala Phe Asn 370 375 380 Phe His Leu Asn Ile Asn Asn Gly Leu Ser His Gln Phe Glu Lys Phe 385 390 395 400 Val Pro Ile Met Lys Glu Tyr Lys Glu Pro Lys Ile Thr Asp Asn Asp 405 410 415 Leu Glu Ala Ile Ser Thr Lys Glu Thr Gly Leu Ala Ser Gln Leu Ser 420 425 430 Gly His Trp Phe Phe Gln Leu Ser Leu Phe Asn Lys Thr Asn Phe Asn 435 440 445 Pro Asn Lys Ile Trp Ile Pro Leu Glu Phe Asn Lys Arg Ser Lys Ile 450 455 460 Lys Phe Asp Lys Asp Leu Glu Ile Tyr Phe Asp Ser His Glu Ser Phe 465 470 475 480 Asn Ile Ser Lys Lys Tyr Leu Gln Glu Ile Asp Gln Glu Ser Leu Lys 485 490 495 Lys Ile Lys Gln Ser Lys Asp Phe Phe Ser Ile Gln Lys Ile Glu Ser 500 505 510 Lys His Asp Asn Asn Asp Ile Leu Gln Leu Glu Phe Phe Glu Asn Asp 515 520 525 Thr Ser Phe Leu Phe Ala Lys Gly Ser Phe Ala Glu Ile Leu Glu Tyr 530 535 540 Asn Met Gln Leu Lys Ile Asp Ser Leu Ile Thr Lys Glu Phe Asn Lys 545 550 555 560 Leu Leu Ala Ile Val Gln Asp Ser Pro Gln Asp Ser Tyr Gln Leu Lys 565 570 575 Ile Arg Val Arg His Asn Asn Lys Leu Pro Arg Glu Lys Tyr Thr Glu 580 585 590 His Glu Ile Lys Leu Glu Val Tyr Asp Cys Arg Lys Ser His Asp His

595 600 605 Asn Glu Pro Ile Ile Leu Ser Gln Gln Ser Thr Gly Phe Gln Trp Ala 610 615 620 Phe Asn Phe Met Phe Gly Phe Leu Tyr Asn Val Gly Ser His Phe Ser 625 630 635 640 Phe Asn His Asn Ile Ile Tyr Val Met Asp Glu Pro Ala Thr His Leu 645 650 655 Ser Val Pro Ala Arg Lys Glu Phe Arg Lys Phe Leu Lys Glu Tyr Ala 660 665 670 His Lys Asn His Val Thr Phe Val Leu Ala Thr His Asp Pro Phe Leu 675 680 685 Val Asp Thr Asp His Leu Asp Glu Ile Arg Ile Val Glu Lys Glu Thr 690 695 700 Glu Gly Ser Val Ile Lys Asn His Phe Asn Tyr Pro Leu Asn Asn Ala 705 710 715 720 Ser Lys Asp Ser Asp Ala Leu Asp Lys Ile Lys Arg Ser Leu Gly Val 725 730 735 Gly Gln His Val Phe His Asn Pro Gln Lys His Arg Ile Ile Phe Val 740 745 750 Glu Gly Ile Thr Asp Tyr Cys Tyr Leu Ser Ala Phe Lys Leu Tyr Leu 755 760 765 Arg Tyr Lys Glu Tyr Lys Asp Asn Pro Ile Pro Phe Thr Phe Leu Pro 770 775 780 Ile Ser Gly Leu Lys Asn Asp Ser Asn Asp Met Lys Glu Thr Ile Glu 785 790 795 800 Lys Leu Cys Glu Leu Asp Asn His Pro Ile Val Leu Thr Asp Asp Asp 805 810 815 Arg Lys Cys Val Phe Asn Gln Gln Ala Thr Ser Glu Arg Phe Lys Arg 820 825 830 Ala Asn Glu Glu Met His Asp Pro Ile Thr Ile Leu Gln Leu Ser Asp 835 840 845 Cys Asp Arg His Phe Lys Gln Ile Glu Asp Cys Phe Ser Ala Asn Asp 850 855 860 Arg Asn Lys Tyr Ala Lys Asn Lys Gln Met Glu Leu Ser Met Ala Phe 865 870 875 880 Lys Thr Arg Leu Leu Tyr Gly Gly Glu Asp Ala Ile Glu Lys Gln Thr 885 890 895 Lys Arg Asn Phe Leu Lys Leu Phe Lys Trp Ile Ala Trp Ala Thr Asn 900 905 910 Leu Ile Lys Asn 915 (2) INFORMATION FOR SEQ ID NO:98: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 176 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori

(ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...176 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:98: Met Thr Ala Met Met Arg Tyr Phe His Ile Tyr Ala Thr Thr Phe Phe 1 5 10 15 Phe Pro Leu Ala Leu Leu Phe Ala Val Ser Gly Leu Ser Leu Leu Phe 20 25 30 Lys Ala Arg Gln Asp Thr Gly Ala Lys Ile Lys Glu Trp Val Leu Glu 35 40 45 Lys Ser Leu Lys Lys Glu Glu Arg Leu Asp Phe Leu Lys Gly Phe Ile 50 55 60 Lys Glu Asn His Ile Ala Met Pro Lys Lys Ile Glu Pro Arg Glu Tyr 65 70 75 80 Arg Gly Ala Leu Val Ile Gly Thr Pro Leu Tyr Glu Ile Asn Leu Glu 85 90 95 Thr Lys Gly Thr Gln Thr Lys Ile Lys Thr Ile Glu Arg Gly Phe Leu 100 105 110 Gly Ala Leu Ile Met Leu His Lys Ala Lys Val Gly Ile Val Phe Gln 115 120 125 Ala Leu Leu Gly Ile Phe Cys Val Phe Leu Leu Leu Phe Tyr Leu Ser 130 135 140 Ala Phe Leu Met Val Ala Phe Lys Asp Thr Lys Arg Met Phe Ile Ser 145 150 155 160 Val Leu Ile Gly Ser Val Val Phe Phe Gly Ala Ile Tyr Trp Ser Leu 165 170 175 (2) INFORMATION FOR SEQ ID NO:99: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 222 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...222 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:99: Met Phe Lys Asn Ala Leu Asn Ile Gln Asp Phe Ser Phe Lys Asn His 1 5 10 15 Thr Ser Thr Ala Ile Ile Gly Thr Asn Gly Ala Gly Lys Ser Thr Leu 20 25 30 Ile Asn Thr Ile Leu Gly Ile Arg Ser Asp Tyr Asn Phe Lys Ala Gln

35 40 45 Asn Asn Asn Ile Pro Tyr His Asp Asn Val Ile Pro Gln Arg Lys Gln 50 55 60 Leu Gly Val Val Ser Asn Leu Phe Asn Tyr Pro Pro Gly Leu Asn Ala 65 70 75 80 Asn Asp Leu Phe Lys Phe Tyr Gln Phe Phe His Lys Asn Cys Thr Leu 85 90 95 Asp Leu Phe Glu Lys Asn Leu Leu Asn Lys Thr Tyr Glu His Leu Ser 100 105 110 Asp Gly Gln Lys Gln Arg Leu Lys Ile Asp Leu Ala Leu Ser His His 115 120 125 Pro Gln Leu Val Ile Met Asp Glu Pro Glu Thr Ser Leu Glu Gln Asn 130 135 140 Ala Leu Ile Arg Leu Ser Asn Leu Ile Ser Leu Arg Asn Thr Gln Gln 145 150 155 160 Leu Thr Ser Ile Ile Ala Thr His Asp Pro Ile Val Leu Asp Ser Cys 165 170 175 Glu Trp Val Leu Leu Leu Lys Asn Gly Asn Ile Ala Gln Tyr Lys Pro 180 185 190 Leu Asn Ser Ile Leu Lys Ser Val Ala Lys Thr Phe Asn Phe Lys Glu 195 200 205 Lys Pro Thr Thr Lys Asp Leu Leu Ala Leu Leu Lys Asp Ile 210 215 220 (2) INFORMATION FOR SEQ ID NO:100: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 406 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...406 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:100: Met Tyr Ala Ala His Pro Ile Lys Pro Ile Lys Ala Pro Lys Leu Lys 1 5 10 15 Ser Gln Phe Leu Arg Arg Val Phe Val Gly Ala Ser Ile Arg Arg Trp 20 25 30 Asn Asp Gln Ala Cys Pro Leu Glu Phe Val Glu Leu Asp Lys Gln Ala 35 40 45 His Lys Ala Met Ile Ala Tyr Leu Leu Ala Lys Asp Leu Lys Asp Arg 50 55 60 Gly Lys Asp Leu Asp Leu Asp Leu Leu Ile Lys Tyr Phe Cys Phe Glu 65 70 75 80 Phe Leu Glu Arg Leu Val Leu Thr Asp Ile Lys Pro Pro Ile Phe Tyr

85 90 95 Ala Leu Gln Gln Thr His Ser Lys Glu Leu Ala Ser Tyr Val Ala Gln 100 105 110 Ser Leu Gln Asp Glu Ile Ser Ala Tyr Phe Ser Leu Glu Glu Leu Lys 115 120 125 Glu Tyr Leu Ser His Arg Pro Gln Ile Leu Glu Thr Gln Ile Leu Glu 130 135 140 Ser Ala His Phe Tyr Ala Ser Lys Trp Glu Phe Asp Ile Ile Tyr His 145 150 155 160 Phe Asn Pro Asn Met Tyr Gly Val Lys Glu Ile Lys Asp Lys Ile Asp 165 170 175 Lys Gln Leu His Asn Asn Asp His Leu Phe Glu Gly Leu Phe Gly Glu 180 185 190 Lys Glu Asp Leu Lys Lys Leu Val Ser Met Phe Gly Gln Leu Arg Phe 195 200 205 Gln Lys Arg Trp Ser Gln Thr Pro Arg Val Pro Gln Thr Ser Val Leu 210 215 220 Gly His Thr Leu Cys Val Ala Ile Met Gly Tyr Leu Leu Ser Phe Asp 225 230 235 240 Leu Lys Ala Cys Lys Ser Met Arg Ile Asn His Phe Leu Gly Gly Leu 245 250 255 Phe His Asp Leu Pro Glu Ile Leu Thr Arg Asp Ile Ile Thr Pro Ile 260 265 270 Lys Gln Ser Val Ala Gly Leu Asp His Cys Ile Lys Glu Ile Glu Lys 275 280 285 Lys Glu Met Gln Asn Lys Val Tyr Ser Phe Val Ser Leu Gly Val Gln 290 295 300 Glu Asp Leu Lys Tyr Phe Thr Glu Asn Glu Phe Lys Asn Arg Tyr Lys 305 310 315 320 Asp Lys Ser His Gln Ile Val Phe Thr Lys Asp Ala Glu Glu Leu Phe 325 330 335 Thr Leu Tyr Asn Ser Asp Glu Tyr Leu Gly Val Cys Gly Glu Leu Leu 340 345 350 Lys Val Cys Asp His Leu Ser Ala Phe Leu Glu Ala Gln Ile Ser Leu 355 360 365 Ser His Gly Ile Ser Ser Tyr Asp Leu Ile Gln Gly Ala Lys Asn Leu 370 375 380 Leu Glu Leu Arg Ser Gln Thr Glu Leu Leu Asp Leu Asp Leu Gly Lys 385 390 395 400 Leu Phe Arg Asp Phe Lys 405 (2) INFORMATION FOR SEQ ID NO:101: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 335 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori

(ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...335 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:101: Val Leu Trp Val Leu Tyr Phe Leu Thr Ser Leu Phe Ile Cys Ser Leu 1 5 10 15 Ile Val Leu Trp Ser Lys Lys Ser Met Leu Phe Val Asp Asn Ala Asn 20 25 30 Lys Ile Gln Gly Phe His His Ala Arg Thr Pro Arg Ala Gly Gly Leu 35 40 45 Gly Ile Phe Leu Ser Phe Ala Leu Ala Cys Tyr Leu Glu Pro Phe Glu 50 55 60 Met Pro Phe Lys Gly Pro Phe Val Phe Leu Gly Leu Ser Leu Val Phe 65 70 75 80 Leu Ser Gly Phe Leu Glu Asp Ile Asn Leu Ser Leu Ser Pro Lys Ile 85 90 95 Arg Leu Ile Leu Gln Ala Val Gly Val Val Cys Ile Ile Ser Ser Thr 100 105 110 Pro Leu Val Val Ser Asp Phe Ser Pro Leu Phe Ser Leu Pro Tyr Phe 115 120 125 Ile Ala Phe Leu Phe Ala Ile Phe Met Leu Val Gly Ile Ser Asn Ala 130 135 140 Ile Asn Ile Ile Asp Gly Phe Asn Gly Leu Ala Ser Gly Ile Cys Ala 145 150 155 160 Ile Ala Leu Leu Val Ile His Tyr Ile Asp Pro Ser Ser Leu Ser Cys 165 170 175 Leu Leu Ala Tyr Met Val Leu Gly Phe Met Val Leu Asn Phe Pro Ser 180 185 190 Gly Lys Ile Phe Leu Gly Asp Gly Gly Ala Tyr Phe Leu Gly Leu Val 195 200 205 Cys Gly Ile Ser Leu Leu His Leu Ser Leu Glu Gln Lys Ile Ser Val 210 215 220 Phe Phe Gly Leu Asn Leu Met Leu Tyr Pro Val Ile Glu Val Leu Phe 225 230 235 240 Ser Ile Leu Arg Arg Lys Ile Lys Arg Gln Lys Ala Thr Met Pro Asp 245 250 255 Asn Leu His Leu His Thr Leu Leu Phe Lys Phe Leu Gln Gln Arg Ser 260 265 270 Phe Asn Tyr Pro Asn Pro Leu Cys Ala Phe Ile Leu Ile Leu Cys Asn 275 280 285 Leu Pro Phe Ile Leu Ile Ser Val Leu Phe Arg Leu Asp Ala Tyr Ala 290 295 300 Leu Ile Val Ile Ser Leu Val Phe Ile Ala Cys Tyr Leu Ile Gly Tyr 305 310 315 320 Ala Tyr Leu Asn Arg Gln Val Cys Ala Leu Glu Lys Arg Ala Phe 325 330 335 (2) INFORMATION FOR SEQ ID NO:102: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 96 amino acids (B) TYPE: amino acid

(D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...96 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:102: Met Lys Lys Val Ile Val Ala Leu Gly Val Leu Ala Phe Ala Asn Val 1 5 10 15 Leu Met Ala Thr Asp Val Lys Ala Leu Val Lys Gly Cys Ala Ala Cys 20 25 30 His Gly Val Lys Phe Glu Lys Lys Ala Leu Gly Lys Ser Lys Ile Val 35 40 45 Asn Met Met Ser Glu Lys Glu Ile Glu Glu Asp Leu Met Ala Phe Lys 50 55 60 Ser Gly Ala Asn Lys Asn Pro Val Met Thr Ala Gln Ala Lys Lys Leu 65 70 75 80 Ser Asp Glu Asp Ile Lys Ala Leu Ala Lys Tyr Ile Pro Thr Leu Lys 85 90 95 (2) INFORMATION FOR SEQ ID NO:103: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 156 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...156 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:103: Met Arg Asp Phe Asn Asn Ile Gln Ile Thr Arg Leu Lys Val Arg Gln 1 5 10 15 Asn Ala Val Phe Glu Lys Leu Asp Leu Glu Phe Lys Asp Gly Leu Ser 20 25 30 Ala Ile Ser Gly Ala Ser Gly Val Gly Lys Ser Val Leu Ile Ala Ser 35 40 45 Leu Leu Gly Ala Phe Gly Leu Lys Glu Ser Asn Ala Ser Asn Ile Glu

50 55 60 Val Glu Leu Ile Ala Pro Phe Leu Asp Thr Glu Glu Tyr Gly Ile Phe 65 70 75 80 Arg Glu Asp Glu His Glu Pro Leu Val Ile Ser Val Ile Lys Lys Glu 85 90 95 Lys Thr Arg Tyr Phe Leu Asn Gln Thr Ser Leu Ser Lys Asn Thr Leu 100 105 110 Lys Ala Leu Leu Lys Gly Leu Ile Lys Arg Leu Ser Asn Asp Arg Phe 115 120 125 Ser Gln Asn Glu Leu Asn Asp Ile Leu Met Leu Ser Leu Leu Asp Gly 130 135 140 Tyr Ile Gln Asn Lys Asn Arg Arg Leu Ala Pro Phe 145 150 155 (2) INFORMATION FOR SEQ ID NO:104: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 118 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...118 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:104: Val Met Leu Met Ala Ile Phe Thr Pro Tyr Ile Leu Ile Leu Lys Met 1 5 10 15 Met Lys Lys Ser Met Ser Leu Phe Ala Asn Met Gly Leu Glu Gln Ile 20 25 30 Phe Cys Asn Arg Asp Ile Lys Asp Leu Asn Asp Phe Val Phe Gly Ile 35 40 45 Glu Val Gly Leu Asp Ser Asn Ala Arg Lys Asn Arg Ser Arg Lys Ala 50 55 60 Met Glu Asn His Leu Ile Gly Leu Phe Val Gln Ala Gln Leu Asn Phe 65 70 75 80 Lys Glu Gln Val Asp Ile Arg Glu Phe Glu Asp Leu Arg Gln Ala Phe 85 90 95 Gly Asn Asp Thr Lys Lys Phe Asp Phe Val Ile Phe Ser Lys Glu Lys 100 105 110 Thr Tyr Phe His Arg Ser 115 (2) INFORMATION FOR SEQ ID NO:105: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 355 amino acids

(B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...355 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:105: Met Asn Ile Lys Ile Leu Lys Ile Leu Val Gly Gly Leu Phe Phe Leu 1 5 10 15 Ser Leu Asn Ala His Leu Trp Gly Lys Gln Asp Asn Ser Phe Leu Gly 20 25 30 Ile Gly Glu Arg Ala Tyr Lys Ser Gly Asn Tyr Ser Lys Ala Ala Ser 35 40 45 Tyr Phe Lys Lys Ala Cys Asn Asp Gly Val Ser Glu Gly Cys Thr Gln 50 55 60 Leu Gly Ile Ile Tyr Glu Asn Gly Gln Gly Thr Arg Ile Asp Tyr Lys 65 70 75 80 Lys Ala Leu Glu Tyr Tyr Lys Thr Ala Cys Gln Ala Asp Asp Arg Glu 85 90 95 Gly Cys Phe Gly Leu Gly Gly Leu Tyr Asp Glu Gly Leu Gly Thr Ala 100 105 110 Gln Asn Tyr Gln Glu Ala Ile Asp Ala Tyr Ala Lys Ala Cys Val Leu 115 120 125 Lys His Pro Glu Ser Cys Tyr Asn Leu Gly Ile Ile Tyr Asp Arg Lys 130 135 140 Ile Lys Gly Asn Ala Ala Gln Ala Val Thr Tyr Tyr Gln Lys Ser Cys 145 150 155 160 Asn Phe Asp Met Ala Lys Gly Cys Tyr Ile Leu Gly Thr Ala Tyr Glu 165 170 175 Lys Gly Phe Leu Glu Val Lys Gln Ser Asn His Lys Ala Val Ile Tyr 180 185 190 Tyr Leu Lys Ala Cys Arg Leu Asn Glu Gly Gln Ala Cys Arg Ala Leu 195 200 205 Gly Ser Leu Phe Glu Asn Gly Asp Ala Gly Leu Asp Glu Asp Phe Glu 210 215 220 Val Ala Phe Asp Tyr Leu Gln Lys Ala Cys Ala Leu Asn Asn Ser Gly 225 230 235 240 Gly Cys Ala Ser Leu Gly Ser Met Tyr Met Leu Gly Arg Tyr Val Lys 245 250 255 Lys Asp Pro Gln Lys Ala Phe Asn Tyr Phe Lys Gln Ala Cys Asp Met 260 265 270 Gly Ser Ala Val Ser Cys Ser Arg Met Gly Phe Met Tyr Ser Gln Gly 275 280 285 Asp Thr Val Ser Lys Asp Leu Arg Lys Ala Leu Asp Asn Tyr Glu Arg 290 295 300 Gly Cys Asp Met Gly Asp Glu Val Gly Cys Phe Ala Leu Ala Gly Met

305 310 315 320 Tyr Tyr Asn Met Lys Asp Lys Glu Asn Ala Ile Met Ile Tyr Asp Lys 325 330 335 Gly Cys Lys Leu Gly Met Lys Gln Ala Cys Glu Asn Leu Thr Lys Leu 340 345 350 Arg Gly Tyr 355 (2) INFORMATION FOR SEQ ID NO:106: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 193 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...193 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:106: Met Lys Glu Lys Asn Phe Trp Pro Leu Gly Ile Met Ser Val Leu Ile 1 5 10 15 Phe Gly Leu Gly Ile Val Val Phe Leu Val Val Phe Ala Leu Lys Asn 20 25 30 Ser Pro Lys Asn Asp Leu Val Tyr Phe Lys Gly His Asn Glu Val Asp 35 40 45 Leu Asn Phe Asn Ala Met Leu Lys Thr Tyr Glu Asn Phe Lys Ser Asn 50 55 60 Tyr Arg Phe Ser Val Gly Leu Lys Pro Leu Thr Glu Ser Pro Lys Thr 65 70 75 80 Pro Ile Leu Pro Tyr Phe Ser Lys Gly Thr His Gly Asp Lys Lys Ile 85 90 95 Gln Glu Asn Leu Leu Asn Asn Ala Leu Ile Leu Glu Lys Ser Asn Thr 100 105 110 Leu Tyr Ala Gln Leu Gln Pro Leu Lys Pro Ala Leu Asp Ser Pro Asn 115 120 125 Ile Gln Val Tyr Leu Ala Phe Tyr Pro Ser Gln Ser Gln Pro Arg Leu 130 135 140 Leu Gly Thr Leu Asp Cys Lys Asn Ala Cys Glu Pro Leu Lys Phe Asp 145 150 155 160 Leu Leu Glu Gly Asp Lys Val Gly Arg Tyr Lys Ile Leu Phe Lys Phe 165 170 175 Val Phe Lys Asn Lys Glu Glu Leu Ile Leu Glu Gln Leu Ala Phe Phe 180 185 190 Lys

(2) INFORMATION FOR SEQ ID NO:107: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 289 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...289 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:107: Leu Gly Ile Asn Met Cys Ser Lys Lys Ile Arg Asn Leu Ile Leu Cys 1 5 10 15 Phe Gly Phe Ile Leu Ser Leu Cys Ala Glu Glu Asn Ile Thr Lys Glu 20 25 30 Asn Met Thr Glu Thr Asn Thr Thr Glu Glu Asn Thr Pro Lys Asp Ala 35 40 45 Pro Ile Leu Leu Glu Glu Lys Arg Ala Gln Thr Leu Glu Leu Lys Glu 50 55 60 Glu Asn Glu Val Ala Lys Lys Ile Asp Glu Lys Ser Leu Leu Glu Glu 65 70 75 80 Ile His Lys Lys Lys Arg Gln Leu Tyr Met Leu Lys Gly Glu Leu His 85 90 95 Glu Lys Asn Glu Ser Ile Leu Phe Gln Gln Met Ala Lys Asn Lys Ser 100 105 110 Gly Phe Phe Ile Gly Val Ile Leu Gly Asp Ile Gly Ile Asn Ala Asn 115 120 125 Pro Tyr Glu Lys Phe Glu Leu Leu Ser Asn Ile Gln Ala Ser Pro Leu 130 135 140 Leu Tyr Gly Leu Arg Ser Gly Tyr Gln Lys Tyr Phe Ala Asn Gly Ile 145 150 155 160 Ser Ala Leu Arg Phe Tyr Gly Glu Tyr Leu Gly Gly Ala Met Lys Gly 165 170 175 Phe Lys Ser Asp Ser Leu Ala Ser Tyr Gln Thr Ala Ser Leu Asn Ile 180 185 190 Asp Leu Leu Met Asp Lys Pro Ile Asp Lys Glu Lys Arg Phe Ala Leu 195 200 205 Gly Ile Phe Gly Gly Val Gly Val Gly Trp Asn Gly Met Tyr Gln Asn 210 215 220 Leu Lys Glu Ile Arg Gly Tyr Ser Gln Pro Asn Ala Phe Gly Leu Val 225 230 235 240 Leu Asn Leu Gly Val Ser Met Thr Leu Asn Leu Lys His Arg Phe Glu 245 250 255 Leu Ala Leu Lys Met Pro Pro Leu Lys Glu Thr Ser Gln Thr Phe Leu 260 265 270 Tyr Tyr Phe Lys Ser Thr Asn Ile Tyr Tyr Ile Ser Tyr Asn Tyr Leu

275 280 285 Leu (2) INFORMATION FOR SEQ ID NO:108: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 668 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...668 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:108: Met Arg Lys Leu Phe Ile Pro Leu Leu Leu Phe Ser Ala Leu Glu Ala 1 5 10 15 Asn Glu Lys Asn Gly Phe Phe Ile Glu Ala Gly Phe Glu Thr Gly Leu 20 25 30 Leu Glu Gly Thr Gln Thr Gln Glu Lys Arg His Thr Thr Thr Lys Asn 35 40 45 Thr Tyr Ala Thr Tyr Asn Tyr Leu Pro Thr Asp Thr Ile Leu Lys Arg 50 55 60 Ala Ala Asn Leu Phe Thr Asn Ala Glu Ala Ile Ser Lys Leu Lys Phe 65 70 75 80 Ser Ser Leu Ser Pro Val Arg Val Leu Tyr Met Tyr Asn Gly Gln Leu 85 90 95 Thr Ile Glu Asn Phe Leu Pro Tyr Asn Leu Asn Asn Val Lys Leu Ser 100 105 110 Phe Thr Asp Ala Gln Gly Asn Thr Ile Asp Leu Gly Val Ile Glu Thr 115 120 125 Ile Pro Lys His Ser Lys Ile Val Leu Pro Gly Glu Ala Phe Asp Ser 130 135 140 Leu Lys Glu Ala Phe Asp Lys Ile Asp Pro Tyr Thr Leu Phe Leu Pro 145 150 155 160 Lys Phe Glu Ala Thr Ser Thr Ser Ile Ser Asp Thr Asn Thr Gln Arg 165 170 175 Val Phe Glu Thr Leu Asn Asn Ile Lys Thr Asn Leu Ile Met Lys Tyr 180 185 190 Ser Asn Glu Asn Pro Asn Asn Phe Asn Thr Cys Pro Tyr Asn Asn Asn 195 200 205 Gly Asn Thr Lys Asn Asp Cys Trp Gln Asn Phe Thr Pro Gln Thr Ala 210 215 220 Glu Glu Phe Thr Asn Leu Met Leu Asn Met Ile Ala Val Leu Asp Ser 225 230 235 240 Gln Ser Trp Gly Asp Ala Ile Leu Asn Ala Pro Phe Glu Phe Thr Asn

245 250 255 Ser Ser Thr Asp Cys Asp Ser Asp Pro Ser Lys Cys Val Asn Pro Gly 260 265 270 Val Asn Gly Arg Val Asp Thr Lys Val Asp Gln Gln Tyr Ile Leu Asn 275 280 285 Lys Gln Gly Ile Ile Asn Asn Phe Arg Lys Lys Ile Glu Ile Asp Ala 290 295 300 Val Val Leu Lys Asn Ser Gly Val Val Gly Leu Ala Asn Gly Tyr Gly 305 310 315 320 Asn Asp Gly Glu Tyr Gly Thr Leu Gly Val Glu Ala Tyr Ala Leu Asp 325 330 335 Pro Lys Lys Leu Phe Gly Asn Asp Leu Lys Thr Ile Asn Leu Glu Asp 340 345 350 Leu Arg Thr Ile Leu His Glu Phe Ser His Thr Lys Gly Tyr Gly His 355 360 365 Asn Gly Asn Met Thr Tyr Gln Arg Val Pro Val Thr Lys Asp Gly Gln 370 375 380 Val Glu Lys Asp Ser Asn Gly Lys Pro Lys Asp Ser Asp Gly Leu Pro 385 390 395 400 Tyr Asn Val Cys Ser Leu Tyr Gly Gly Ser Asn Gln Pro Ala Phe Pro 405 410 415 Ser Asn Tyr Pro Asn Ser Ile Tyr His Asn Cys Ala Asp Val Pro Ala 420 425 430 Gly Phe Leu Gly Val Thr Ala Ala Val Trp Gln Gln Leu Ile Asn Gln 435 440 445 Asn Ala Leu Pro Ile Asn Tyr Ala Asn Leu Gly Ser Gln Thr Asn Tyr 450 455 460 Asn Leu Asn Ala Ser Leu Asn Thr Gln Asp Leu Ala Asn Ser Met Leu 465 470 475 480 Ser Thr Ile Gln Lys Thr Phe Val Thr Ser Ser Val Thr Asn His His 485 490 495 Phe Ser Asn Ala Ser Gln Ser Phe Arg Ser Pro Ile Leu Gly Val Asn 500 505 510 Ala Lys Ile Gly Tyr Gln Asn Tyr Phe Asn Asp Phe Ile Gly Leu Ala 515 520 525 Tyr Tyr Gly Ile Ile Lys Tyr Asn Tyr Ala Lys Ala Val Asn Gln Lys 530 535 540 Val Gln Gln Leu Ser Tyr Gly Gly Gly Ile Asp Leu Leu Leu Asp Phe 545 550 555 560 Ile Thr Thr Tyr Ser Asn Lys Asn Ser Pro Thr Gly Ile Gln Thr Lys 565 570 575 Arg Asn Phe Ser Ser Ser Phe Gly Ile Phe Gly Gly Leu Arg Gly Leu 580 585 590 Tyr Asn Ser Tyr Tyr Val Leu Asn Lys Val Lys Gly Ser Gly Asn Leu 595 600 605 Asp Val Ala Thr Gly Leu Asn Tyr Arg Tyr Lys His Ser Lys Tyr Ser 610 615 620 Val Gly Ile Ser Ile Pro Leu Ile Gln Arg Lys Ala Ser Val Val Ser 625 630 635 640 Ser Gly Gly Asp Tyr Thr Asn Ser Phe Val Phe Asn Glu Gly Ala Ser 645 650 655 His Phe Lys Val Phe Phe Asn Tyr Gly Trp Val Phe 660 665 (2) INFORMATION FOR SEQ ID NO:109:

(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 63 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...63 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:109: Met Asn Thr Glu Ile Leu Thr Ile Met Leu Val Val Ser Val Leu Met 1 5 10 15 Gly Leu Val Gly Leu Ile Ala Phe Leu Trp Gly Val Lys Ser Gly Gln 20 25 30 Phe Asp Asp Glu Lys Arg Met Leu Glu Ser Val Leu Tyr Asp Ser Ala 35 40 45 Ser Asp Leu Asn Glu Ala Ile Leu Gln Glu Lys Arg Gln Lys Asn 50 55 60 (2) INFORMATION FOR SEQ ID NO:110: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 406 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...406 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:110: Met Val Phe Phe His Lys Lys Ile Ile Leu Asn Phe Ile Tyr Ser Leu 1 5 10 15 Met Val Ala Phe Leu Phe His Leu Ser Tyr Gly Val Leu Leu Lys Ala 20 25 30 Asp Gly Met Ala Lys Lys Gln Thr Leu Leu Val Gly Glu Arg Leu Val 35 40 45 Trp Asp Lys Leu Thr Leu Leu Gly Phe Leu Glu Lys Asn His Ile Pro

50 55 60 Gln Lys Leu Tyr Tyr Asn Leu Ser Ser Gln Asp Lys Glu Leu Ser Ala 65 70 75 80 Glu Ile Gln Ser Asn Val Thr Tyr Tyr Thr Leu Arg Asp Ala Asn Asn 85 90 95 Thr Leu Ile Gln Ala Leu Ile Pro Ile Ser Gln Asp Leu Gln Ile His 100 105 110 Ile Tyr Lys Lys Gly Glu Asp Tyr Phe Leu Asp Phe Ile Pro Ile Val 115 120 125 Phe Thr Arg Lys Glu Arg Thr Leu Leu Leu Ser Leu Gln Thr Ser Pro 130 135 140 Tyr Gln Asp Ile Val Lys Ala Thr Asn Asp Pro Leu Leu Ala Asn Gln 145 150 155 160 Leu Met Asn Ala Tyr Lys Lys Ser Val Pro Phe Lys Arg Leu Val Lys 165 170 175 Asn Asp Lys Ile Ala Ile Val Tyr Thr Arg Asp Tyr Arg Val Gly Gln 180 185 190 Ala Phe Gly Gln Pro Thr Ile Lys Met Ala Met Val Ser Ser Arg Leu 195 200 205 His Gln Tyr Tyr Leu Phe Ser His Ser Asn Gly Arg Tyr Tyr Asp Ser 210 215 220 Lys Ala Gln Glu Val Ala Gly Phe Leu Leu Glu Thr Pro Val Lys Tyr 225 230 235 240 Thr Arg Ile Ser Ser Pro Phe Ser Tyr Gly Arg Phe His Pro Val Leu 245 250 255 Lys Val Lys Arg Pro His Tyr Gly Val Asp Tyr Ala Ala Lys His Gly 260 265 270 Ser Leu Ile His Ser Ala Ser Asp Gly Arg Val Gly Phe Ile Gly Val 275 280 285 Lys Ala Gly Tyr Gly Lys Val Val Glu Ile His Leu Asn Glu Leu Arg 290 295 300 Leu Val Tyr Ala His Met Ser Ala Phe Ala Asn Gly Leu Lys Lys Gly 305 310 315 320 Ser Phe Val Lys Lys Gly Gln Ile Ile Gly Arg Val Gly Ser Thr Gly 325 330 335 Leu Ser Thr Gly Pro His Leu His Phe Gly Val Tyr Lys Asn Ser Arg 340 345 350 Pro Ile Asn Pro Leu Gly Tyr Ile Arg Thr Ala Lys Ser Lys Leu His 355 360 365 Gly Lys Gln Arg Glu Val Phe Leu Glu Lys Ala Gln Tyr Ser Lys Gln 370 375 380 Lys Leu Glu Glu Leu Phe Lys Thr His Ser Phe Glu Lys Asn Ser Phe 385 390 395 400 Tyr Leu Leu Glu Gly Phe 405 (2) INFORMATION FOR SEQ ID NO:111: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 296 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein

(iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...296 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:lll: Leu Phe Leu Val Lys Lys Ile Gly Val Val Ile Met Ile Leu Val Cys 1 5 10 15 Phe Leu Ala Cys Ser Gln Glu Ser Phe Ile Lys Met Gln Lys Lys Ala 20 25 30 Gln Glu Gln Glu Asn Asp Gly Ser Lys Arg Pro Ser Tyr Val Asp Ser 35 40 45 Asp Tyr Glu Val Phe Ser Glu Thr Ile Phe Leu Gln Asn Met Val Tyr 50 55 60 Gln Pro Ile Glu Glu Arg Asn Ala Phe Phe Gln Leu Thr Lys Asp Glu 65 70 75 80 Asp Asn Ser Phe Asn Pro Glu Asn Ser Val Ile Leu Leu Asn Glu Pro 85 90 95 Ser Asp Asn Ser Glu Lys Asn Leu Leu Ser Tyr Pro Asn Asp Pro Asn 100 105 110 Asn Asn Glu Asp Asn Ala Asn Asn Ser Gln Lys Asn Pro Phe Leu Tyr 115 120 125 Lys Pro Lys Arg Lys Thr Lys Asn Pro Lys Leu Ile Glu Tyr Ser Gln 130 135 140 Gln Asp Phe Tyr Pro Leu Lys Asn Gly Asp Ile Ile Met Ser Lys Glu 145 150 155 160 Gly Asp Gln Trp Leu Ile Glu Ile Gln Ser Lys Ala Leu Lys Arg Phe 165 170 175 Leu Lys Asp Gln Asn Asp Lys Asp Arg Gln Ile Gln Thr Phe Thr Phe 180 185 190 Asn Asp Thr Lys Thr Gln Ile Ala Gln Ile Lys Gly Lys Ile Ser Ser 195 200 205 Tyr Val Tyr Thr Thr Asn Asn Gly Ser Leu Ser Leu Arg Pro Phe Tyr 210 215 220 Glu Ser Phe Leu Leu Glu Lys Lys Ser Asp Asn Val Tyr Thr Ile Glu 225 230 235 240 Asn Lys Ala Leu Asp Thr Met Glu Ile Ser Lys Cys Gln Met Val Leu 245 250 255 Lys Lys His Ser Thr Asp Lys Leu Asp Ser Gln His Lys Ala Ile Ser 260 265 270 Ile Asp Leu Asp Phe Lys Lys Glu Arg Phe Lys Ser Asp Thr Glu Leu 275 280 285 Phe Leu Glu Cys Leu Lys Glu Ser 290 295 (2) INFORMATION FOR SEQ ID NO:112: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 248 amino acids (B) TYPE: amino acid

(D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...248 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:112: Val Ser Tyr Asp Asn Thr Asp Asp Tyr Tyr Phe Pro Arg Asn Gly Val 1 5 10 15 Ile Phe Ser Ser Tyr Ala Thr Met Ser Gly Leu Pro Ser Ser Gly Thr 20 25 30 Leu Asn Ser Trp Asn Gly Leu Gly Gly Asn Val Arg Asn Thr Lys Val 35 40 45 Tyr Gly Lys Phe Ala Ala Tyr His His Leu Gln Lys Tyr Leu Leu Ile 50 55 60 Asp Leu Ile Ala Arg Phe Lys Thr Gln Gly Gly Tyr Ile Phe Arg Tyr 65 70 75 80 Asn Thr Asp Asp Tyr Leu Pro Leu Asn Ser Thr Phe Tyr Met Gly Gly 85 90 95 Val Thr Thr Val Arg Gly Phe Arg Asn Gly Ser Ile Thr Pro Lys Asp 100 105 110 Glu Phe Gly Leu Trp Leu Gly Gly Asp Gly Ile Phe Thr Ala Ser Thr 115 120 125 Glu Leu Ser Tyr Gly Val Leu Lys Ala Ala Lys Met Arg Leu Ala Trp 130 135 140 Phe Phe Asp Phe Gly Phe Leu Thr Phe Lys Thr Pro Thr Arg Gly Ser 145 150 155 160 Phe Phe Tyr Asn Ala Pro Thr Thr Thr Ala Asn Phe Lys Asp Tyr Gly 165 170 175 Val Val Gly Ala Gly Phe Glu Arg Ala Thr Trp Arg Ala Ser Thr Gly 180 185 190 Leu Gln Ile Glu Trp Ile Ser Pro Met Gly Pro Leu Val Leu Ile Phe 195 200 205 Pro Ile Ala Phe Phe Asn Gln Trp Gly Asp Gly Asn Gly Lys Lys Cys 210 215 220 Lys Gly Leu Cys Phe Asn Pro Asn Met Asn Asp Tyr Thr Gln His Phe 225 230 235 240 Glu Phe Ser Met Gly Thr Arg Phe 245 (2) INFORMATION FOR SEQ ID NO:113: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 335 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...335 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:113: Val Gln His Phe Asn Phe Leu Tyr Lys Asp Ser Leu Phe Ser Ile Ala 1 5 10 15 Leu Phe Thr Phe Ile Ile Ala Leu Val Ile Leu Leu Glu Gln Ala Arg 20 25 30 Ala Tyr Phe Thr Arg Lys Arg Asn Lys Lys Phe Leu Gln Lys Phe Ala 35 40 45 Gln Asn Gln Asn Ala Tyr Ala Ser Ser Glu Asn Leu Asp Glu Leu Leu 50 55 60 Lys His Ala Lys Ile Ser Ser Leu Met Phe Leu Ala Arg Ala Tyr Ser 65 70 75 80 Lys Ala Asp Val Glu Met Ser Ile Glu Ile Leu Lys Gly Leu Leu Asn 85 90 95 Arg Pro Leu Lys Asp Glu Glu Lys Ile Ala Val Leu Asp Leu Leu Ala 100 105 110 Lys Asn Tyr Phe Ser Val Gly Tyr Leu Gln Lys Thr Lys Asp Thr Val 115 120 125 Lys Glu Ile Leu Arg Phe Ser Pro Arg Asn Val Glu Ala Leu Leu Lys 130 135 140 Leu Leu His Ala Tyr Glu Leu Glu Lys Asp Tyr Ser Lys Ala Leu Glu 145 150 155 160 Thr Leu Glu Cys Leu Glu Glu Leu Glu Val Pro Lys Ile Glu Thr Ile 165 170 175 Lys Asn Tyr Leu Tyr Leu Met His Leu Ile Glu Asn Lys Glu Asp Ala 180 185 190 Ala Lys Ile Leu His Val Ser Lys Ala Ser Leu Asp Leu Lys Lys Ile 195 200 205 Ala Leu Asn His Leu Lys Ser His Asp Glu Asn Leu Phe Trp Gln Glu 210 215 220 Ile Asp Thr Thr Glu Arg Leu Glu Asn Val Ile Asp Leu Leu Trp Asp 225 230 235 240 Met Asn Ile Pro Ala Phe Ile Leu Glu Lys His Ala Leu Leu Gln Asp 245 250 255 Ile Ala Arg Ser Gln Gly Leu Leu Leu Asp His Lys Pro Cys Gln Ile 260 265 270 Phe Glu Leu Glu Val Leu Arg Ala Leu Leu His Ser Pro Ile Lys Ala 275 280 285 Ser Leu Thr Phe Glu Tyr Arg Cys Lys His Cys Lys Gln Ile Phe Pro 290 295 300 Phe Glu Ser His Arg Cys Pro Val Cys Tyr Gln Leu Ala Phe Met Asp 305 310 315 320 Met Val Leu Lys Ile Ser Lys Lys Thr His Ala Met Gly Val Asp 325 330 335

(2) INFORMATION FOR SEQ ID NO:114: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 413 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...413 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:114: Met Arg Lys Ile Phe Ser Tyr Ile Ser Lys Val Leu Leu Phe Ile Gly 1 5 10 15 Val Val Tyr Ala Glu Pro Asp Ser Lys Val Glu Ala Leu Glu Gly Arg 20 25 30 Lys Gln Glu Ser Ser Leu Asp Lys Lys Ile Arg Gln Glu Leu Lys Ser 35 40 45 Lys Glu Leu Lys Asn Lys Glu Leu Lys Asn Lys Asp Leu Lys Asn Lys 50 55 60 Glu Glu Lys Lys Glu Thr Lys Ala Lys Arg Lys Pro Arg Ala Glu Val 65 70 75 80 His His Gly Asp Ala Lys Asn Pro Thr Pro Lys Ile Thr Pro Pro Lys 85 90 95 Ile Lys Gly Ser Ser Lys Gly Val Gln Asn Gln Gly Val Gln Asn Asn 100 105 110 Ala Pro Lys Pro Glu Glu Lys Asp Thr Thr Pro Gln Ala Thr Glu Lys 115 120 125 Asn Lys Glu Thr Ser Pro Ser Ser Gln Phe Asn Ser Ile Phe Gly Asn 130 135 140 Pro Asn Asn Ala Thr Asn Asn Thr Leu Glu Asp Lys Val Val Gly Gly 145 150 155 160 Ile Ser Leu Leu Val Asn Gly Ser Pro Ile Thr Leu Tyr Gln Ile Gln 165 170 175 Glu Glu Gln Glu Lys Ser Lys Val Ser Lys Ala Gln Ala Arg Asp Arg 180 185 190 Leu Ile Ala Glu Arg Ile Lys Asn Gln Glu Ile Glu Arg Leu Lys Ile 195 200 205 His Val Asp Asp Asp Lys Leu Asp Gln Glu Met Ala Met Met Ala Gln 210 215 220 Gln Gln Gly Met Asp Leu Asp His Phe Lys Gln Met Leu Met Ala Glu 225 230 235 240 Gly His Tyr Lys Leu Tyr Arg Asp Gln Leu Lys Glu His Leu Glu Met 245 250 255 Gln Glu Leu Leu Arg Asn Ile Leu Leu Thr Asn Val Asp Thr Ser Ser 260 265 270

Glu Thr Lys Met Arg Glu Tyr Tyr Asn Lys His Lys Glu Gln Phe Ser 275 280 285 Ile Pro Thr Glu Ile Glu Thr Val Arg Tyr Thr Ser Thr Asn Gln Glu 290 295 300 Asp Leu Glu Arg Ala Met Ala Asp Pro Asn Leu Glu Val Pro Gly Val 305 310 315 320 Ser Lys Ala Asn Glu Lys Ile Glu Met Lys Thr Leu Asn Pro Gln Ile 325 330 335 Ala Gln Val Phe Ile Ser His Glu Gln Gly Ser Phe Thr Pro Val Met 340 345 350 Asn Gly Gly Gly Gly Gln Phe Ile Thr Phe Tyr Ile Lys Glu Lys Arg 355 360 365 Gly Lys Asn Glu Val Ser Phe Ser Gln Ala Lys Gln Phe Ile Ala Gln 370 375 380 Lys Leu Val Glu Glu Ser Lys Asp Lys Ile Leu Glu Glu His Phe Glu 385 390 395 400 Lys Leu Arg Val Lys Ser Arg Ile Val Met Ile Arg Glu 405 410 (2) INFORMATION FOR SEQ ID NO:115: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 186 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...186 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:115: Met Ile Lys Arg Ile Ala Cys Ile Leu Ser Leu Ser Ala Ser Leu Ala 1 5 10 15 Leu Ala Gly Glu Val Asn Gly Phe Phe Met Gly Ala Gly Tyr Gln Gln 20 25 30 Gly Arg Tyr Gly Pro Tyr Asn Ser Asn Tyr Ser Asp Trp Arg His Gly 35 40 45 Asn Asp Leu Tyr Gly Leu Asn Phe Lys Leu Gly Phe Val Gly Phe Ala 50 55 60 Asn Lys Trp Phe Gly Ala Arg Val Tyr Gly Phe Leu Asp Trp Phe Asn 65 70 75 80 Thr Ser Gly Thr Glu His Thr Lys Thr Asn Leu Leu Thr Tyr Gly Gly 85 90 95 Gly Gly Asp Leu Ile Val Asn Leu Ile Pro Leu Asp Lys Phe Ala Leu 100 105 110 Gly Leu Ile Gly Gly Val Gln Leu Ala Gly Asn Thr Trp Met Phe Pro 115 120 125

Tyr Asp Val Asn Gln Thr Arg Phe Gln Phe Leu Trp Asn Leu Gly Gly 130 135 140 Arg Met Arg Val Gly Asp Arg Ser Ala Phe Glu Ala Gly Val Lys Phe 145 150 155 160 Pro Met Val Asn Gln Gly Ser Lys Asp Val Gly Leu Ile Arg Tyr Tyr 165 170 175 Ser Trp Tyr Val Asp Tyr Val Phe Thr Phe 180 185 (2) INFORMATION FOR SEQ ID NO:116: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 242 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...242 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:116: Met Lys Lys Phe Phe Ser Gln Ser Leu Leu Ala Leu Ile Ile Ser Met 1 5 10 15 Asn Ala Val Ser Gly Met Asp Gly Asn Gly Val Phe Leu Gly Ala Gly 20 25 30 Tyr Leu Gln Gly Gln Ala Gln Met His Ala Asp Ile Asn Ser Gln Lys 35 40 45 Gln Ala Thr Asn Ala Thr Ile Lys Gly Phe Asp Ala Leu Leu Gly Tyr 50 55 60 Gln Phe Phe Phe Glu Lys His Phe Gly Leu Arg Leu Tyr Gly Phe Phe 65 70 75 80 Asp Tyr Ala His Ala Asn Ser Ile Lys Leu Lys Asn Pro Asn Tyr Asn 85 90 95 Ser Glu Ala Ala Gln Val Ala Ser Gln Ile Leu Gly Lys Gln Glu Ile 100 105 110 Asn Arg Leu Thr Asn Ile Ala Asp Pro Arg Thr Phe Glu Pro Asn Met 115 120 125 Leu Thr Tyr Gly Gly Ala Met Asp Val Met Val Asn Val Ile Asn Asn 130 135 140 Gly Ile Met Ser Leu Gly Ala Phe Gly Gly Ile Gln Leu Ala Gly Asn 145 150 155 160 Ser Trp Leu Met Ala Thr Pro Ser Phe Glu Gly Ile Leu Val Glu Gln 165 170 175 Ala Leu Val Ser Lys Lys Ala Thr Ser Phe Gln Phe Leu Phe Asn Val 180 185 190 Gly Ala Arg Leu Arg Ile Leu Lys His Ser Ser Ile Glu Ala Gly Val 195 200 205

Lys Phe Pro Met Leu Lys Lys Asn Pro Tyr Ile Thr Ala Lys Asn Leu 210 215 220 Asp Ile Gly Phe Arg Arg Val Tyr Ser Trp Tyr Val Asn Tyr Val Phe 225 230 235 240 Thr Phe (2) INFORMATION FOR SEQ ID NO:117: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 256 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...256 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:117: Met Gly Tyr Ala Ser Lys Leu Ala Leu Lys Ile Cys Leu Val Gly Leu 1 5 10 15 Cys Leu Phe Ser Thr Leu Gly Ala Glu His Leu Glu Gln Lys Gly Asn 20 25 30 Tyr Ile Tyr Lys Gly Glu Glu Ala Tyr Asn Asn Lys Glu Tyr Glu Arg 35 40 45 Ala Ala Ser Phe Tyr Lys Ser Ala Ile Lys Asn Gly Glu Ser Leu Ala 50 55 60 Tyr Ile Leu Leu Gly Ile Met Tyr Glu Asn Gly Arg Gly Val Pro Lys 65 70 75 80 Asp Tyr Lys Lys Ala Val Glu Tyr Phe Gln Lys Ala Val Asp Asn Asp 85 90 95 Ile Pro Arg Gly Tyr Asn Asn Leu Gly Val Met Tyr Lys Glu Gly Lys 100 105 110 Gly Val Pro Lys Asp Glu Lys Lys Ala Val Glu Tyr Phe Arg Ile Ala 115 120 125 Thr Glu Lys Gly Tyr Thr Asn Ala Tyr Ile Asn Leu Gly Ile Met Tyr 130 135 140 Met Glu Gly Arg Gly Val Pro Ser Asn Tyr Ala Lys Ala Thr Glu Cys 145 150 155 160 Phe Arg Lys Ala Met His Lys Gly Asn Val Glu Ala Tyr Ile Leu Leu 165 170 175 Gly Asp Ile Tyr Tyr Ser Gly Asn Asp Gln Leu Gly Ile Glu Pro Asp 180 185 190 Lys Asp Lys Ala Val Val Tyr Tyr Lys Met Ala Ala Asp Val Ser Ser 195 200 205 Ser Arg Ala Tyr Glu Gly Leu Ser Glu Ser Tyr Arg Tyr Gly Leu Gly 210 215 220

Val Glu Lys Asp Lys Lys Lys Ala Glu Glu Tyr Met Gln Lys Ala Cys 225 230 235 240 Asp Phe Asp Ile Asp Lys Asn Cys Lys Lys Lys Asn Thr Ser Ser Arg 245 250 255 (2) INFORMATION FOR SEQ ID NO:118: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 657 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...657 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:118: Met Arg Lys Leu Phe Ile Pro Leu Leu Leu Phe Ser Ala Leu Glu Ala 1 5 10 15 Asn Glu Lys Asn Gly Phe Phe Ile Glu Ala Gly Phe Glu Thr Gly Leu 20 25 30 Leu Glu Gly Thr Gln Thr Gln Glu Lys Arg His Thr Thr Thr Lys Asn 35 40 45 Thr Tyr Ala Thr Tyr Asn Tyr Leu Pro Thr Asp Thr Ile Leu Lys Arg 50 55 60 Ala Ala Asn Leu Phe Thr Asn Ala Glu Ala Ile Ser Lys Leu Lys Phe 65 70 75 80 Ser Ser Leu Ser Pro Val Arg Val Leu Tyr Met Tyr Asn Gly Gln Leu 85 90 95 Thr Ile Glu Asn Phe Leu Pro Tyr Asn Leu Asn Asn Val Lys Leu Ser 100 105 110 Phe Thr Asp Ala Gln Gly Asn Val Ile Asp Leu Gly Val Ile Glu Thr 115 120 125 Ile Pro Lys His Ser Lys Ile Val Leu Pro Gly Glu Ala Phe Asp Ser 130 135 140 Leu Lys Ile Asp Pro Tyr Thr Leu Phe Leu Pro Lys Ile Glu Ala Thr 145 150 155 160 Ser Thr Ser Ile Ser Asp Ala Asn Thr Gln Arg Val Phe Glu Thr Leu 165 170 175 Asn Lys Ile Lys Thr Asn Leu Val Val Asn Tyr Arg Asn Glu Asn Lys 180 185 190 Phe Lys Asp His Glu Asn His Trp Glu Ala Phe Thr Pro Gln Thr Ala 195 200 205 Glu Glu Phe Thr Asn Leu Met Leu Asn Met Ile Ala Val Leu Asp Ser 210 215 220 Gln Ser Trp Gly Asp Ala Ile Leu Asn Ala Pro Phe Glu Phe Thr Asn 225 230 235 240

Ser Pro Thr Asp Cys Asp Asn Asp Pro Ser Lys Cys Val Asn Pro Gly 245 250 255 Thr Asn Gly Leu Val Asn Ser Lys Val Asp Gln Lys Tyr Val Leu Asn 260 265 270 Lys Gln Asp Ile Val Asn Lys Phe Lys Asn Lys Ala Asp Leu Asp Val 275 280 285 Ile Val Leu Lys Asp Ser Gly Val Val Gly Leu Gly Ser Asp Ile Thr 290 295 300 Pro Ser Asn Asn Asp Asp Gly Lys His Tyr Gly Gln Leu Gly Val Val 305 310 315 320 Ala Ser Ala Leu Asp Pro Lys Lys Leu Phe Gly Asp Asn Leu Lys Thr 325 330 335 Ile Asn Leu Glu Asp Leu Arg Thr Ile Leu His Glu Phe Ser His Thr 340 345 350 Lys Gly Tyr Gly His Asn Gly Asn Met Thr Tyr Gln Arg Val Pro Val 355 360 365 Thr Lys Asp Gly Gln Val Glu Lys Asp Ser Asn Gly Lys Pro Lys Asp 370 375 380 Ser Asp Gly Leu Pro Tyr Asn Val Cys Ser Leu Tyr Gly Gly Ser Asn 385 390 395 400 Gln Pro Ala Phe Pro Ser Asn Tyr Pro Asn Ser Ile Tyr His Asn Cys 405 410 415 Ala Asp Val Pro Ala Gly Phe Leu Gly Val Thr Ala Ala Val Trp Gln 420 425 430 Gln Leu Ile Asn Gln Asn Ala Leu Pro Ile Asn Tyr Ala Asn Leu Gly 435 440 445 Ser Gln Thr Asn Tyr Asn Leu Asn Ala Ser Leu Asn Thr Gln Asp Leu 450 455 460 Ala Asn Ser Met Leu Ser Thr Ile Gln Lys Thr Phe Val Thr Ser Ser 465 470 475 480 Val Thr Asn His His Phe Ser Asn Ala Ser Gln Ser Phe Arg Ser Pro 485 490 495 Ile Leu Gly Val Asn Ala Lys Ile Gly Tyr Gln Asn Tyr Phe Asn Asp 500 505 510 Phe Ile Gly Leu Ala Tyr Tyr Gly Ile Ile Lys Tyr Asn Tyr Ala Lys 515 520 525 Ala Val Asn Gln Lys Val Gln Gln Leu Ser Tyr Gly Gly Gly Ile Asp 530 535 540 Leu Leu Leu Asp Phe Ile Thr Thr Tyr Ser Asn Lys Asn Ser Pro Thr 545 550 555 560 Gly Ile Gln Thr Lys Arg Asn Phe Ser Ser Ser Phe Gly Ile Phe Gly 565 570 575 Gly Leu Arg Gly Leu Tyr Asn Ser Tyr Tyr Val Leu Asn Lys Val Lys 580 585 590 Gly Ser Gly Asn Leu Asp Val Ala Thr Gly Leu Asn Tyr Arg Tyr Lys 595 600 605 His Ser Lys Tyr Ser Val Gly Ile Ser Ile Pro Leu Ile Gln Arg Lys 610 615 620 Ala Ser Val Val Ser Ser Gly Gly Asp Tyr Thr Asn Ser Phe Val Phe 625 630 635 640 Asn Glu Gly Ala Ser His Phe Lys Val Phe Phe Asn Tyr Gly Trp Val 645 650 655 Phe

(2) INFORMATION FOR SEQ ID NO:119: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 167 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...167 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:119: Met Lys Leu Val Ser Leu Ile Val Ala Leu Val Phe Cys Cys Phe Leu 1 5 10 15 Gly Ala Val Glu Leu Pro Gly Val Tyr Gln Thr Gln Glu Phe Leu Tyr 20 25 30 Met Lys Ser Ser Phe Val Glu Phe Phe Glu His Asn Gly Lys Phe Tyr 35 40 45 Ala Tyr Gly Ile Ser Asp Val Asp Gly Ser Lys Ala Lys Lys Asp Lys 50 55 60 Leu Asn Pro Asn Pro Lys Leu Arg Asn Arg Ser Asp Lys Gly Val Val 65 70 75 80 Phe Leu Ser Asp Leu Ile Lys Val Gly Glu Gln Ser Tyr Lys Gly Gly 85 90 95 Lys Ala Tyr Asn Phe Tyr Asp Gly Lys Thr Tyr His Val Arg Val Thr 100 105 110 Gln Asn Ser Asn Gly Asp Leu Glu Phe Thr Ser Ser Tyr Asp Lys Trp 115 120 125 Gly Tyr Val Gly Lys Thr Phe Thr Trp Lys Arg Leu Ser Asp Glu Glu 130 135 140 Ile Lys Asn Leu Lys Leu Lys Arg Phe Asn Leu Asp Glu Val Leu Lys 145 150 155 160 Thr Leu Lys Asp Ser Pro Ile 165 (2) INFORMATION FOR SEQ ID NO:120: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 294 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE:

(A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...294 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:120: Met Ser Asn Gln Ala Ser His Leu Asp Asn Phe Met Asn Ala Lys Asn 1 5 10 15 Pro Lys Ser Phe Phe Asp Asn Lys Gly Asn Thr Lys Phe Ile Ala Ile 20 25 30 Thr Ser Gly Lys Gly Gly Val Gly Lys Ser Asn Ile Ser Ala Asn Leu 35 40 45 Ala Tyr Ser Leu Tyr Lys Lys Gly Tyr Lys Val Gly Val Phe Asp Ala 50 55 60 Asp Ile Gly Leu Ala Asn Leu Asp Val Ile Phe Gly Val Lys Thr His 65 70 75 80 Lys Asn Ile Leu His Ala Leu Lys Gly Glu Ala Lys Leu Gln Glu Ile 85 90 95 Ile Cys Glu Ile Glu Pro Gly Leu Cys Leu Ile Pro Gly Asp Ser Gly 100 105 110 Glu Glu Ile Leu Lys Tyr Ile Ser Gly Ala Glu Ala Leu Asp Arg Phe 115 120 125 Val Asp Glu Glu Gly Val Leu Ser Ser Leu Asp Tyr Ile Val Ile Asp 130 135 140 Thr Gly Ala Gly Ile Gly Ala Thr Thr Gln Ala Phe Leu Asn Ala Ser 145 150 155 160 Asp Cys Val Val Ile Val Thr Thr Pro Asp Pro Ser Ala Ile Thr Asp 165 170 175 Ala Tyr Ala Cys Ile Lys Ile Asn Ser Lys Asn Lys Asp Glu Leu Phe 180 185 190 Leu Ile Ala Asn Met Val Ala Gln Pro Lys Glu Gly Arg Ala Thr Tyr 195 200 205 Glu Arg Leu Phe Lys Val Ala Lys Asn Asn Ile Ala Ser Leu Glu Leu 210 215 220 His Tyr Leu Gly Ala Ile Glu Asn Ser Ser Leu Leu Lys Arg Tyr Val 225 230 235 240 Arg Glu Arg Lys Ile Leu Arg Lys Ile Ala Pro Asn Asp Leu Phe Ser 245 250 255 Gln Ser Ile Asp Gln Ile Ala Ser Leu Leu Val Ser Lys Leu Glu Thr 260 265 270 Gly Thr Leu Glu Ile Pro Lys Glu Gly Leu Lys Ser Phe Phe Lys Arg 275 280 285 Leu Leu Lys Tyr Leu Gly 290 (2) INFORMATION FOR SEQ ID NO:121: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 372 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein

(iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...372 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:121: Leu Glu Pro Ser Arg Asn Arg Leu Lys His Ala Ala Phe Phe Val Gly 1 5 10 15 Leu Phe Ile Val Leu Phe Leu Ile Ile Met Lys His Gln Thr Ser Pro 20 25 30 Tyr Ala Phe Thr His Asn Gln Ala Leu Val Thr Gln Thr Pro Pro Tyr 35 40 45 Phe Thr Gln Leu Thr Ile Pro Lys Pro Asn Asp Ala Leu Ser Ala His 50 55 60 Ala Ser Ser Leu Ile Ser Leu Pro Asn Asp Asn Leu Leu Ser Ala Tyr 65 70 75 80 Phe Ser Gly Thr Lys Glu Gly Ala Arg Asp Val Lys Ile Ser Ala Asn 85 90 95 Leu Phe Asp Ser Lys Thr Asn Arg Trp Ser Glu Ala Phe Ile Leu Leu 100 105 110 Thr Lys Glu Glu Leu Ser His His Ser His Glu Tyr Ile Lys Lys Leu 115 120 125 Gly Asn Pro Leu Leu Phe Leu His Asp Asn Lys Ile Leu Leu Phe Val 130 135 140 Val Gly Val Ser Met Gly Gly Trp Ala Thr Ser Lys Ile Tyr Gln Phe 145 150 155 160 Glu Ser Ala Leu Glu Pro Ile His Phe Lys Phe Ala Arg Lys Leu Ser 165 170 175 Leu Ser Pro Phe Leu Asn Leu Ser His Leu Val Arg Asn Lys Pro Leu 180 185 190 Asn Thr Thr Asp Gly Gly Phe Met Leu Pro Leu Tyr His Glu Leu Ala 195 200 205 Thr Gln Tyr Pro Leu Leu Leu Lys Phe Asp Gln Gln Asn Asn Pro Arg 210 215 220 Glu Leu Leu Arg Pro Asn Thr Leu Asn His Gln Leu Gln Pro Ser Leu 225 230 235 240 Thr Pro Phe Lys Asp Cys Ala Val Met Ala Phe Arg Asn His Ser Phe 245 250 255 Lys Asp Ser Leu Met Leu Glu Thr Cys Lys Thr Pro Thr Asp Trp Gln 260 265 270 Lys Pro Ile Ser Thr Asn Leu Lys Asn Leu Asp Asp Ser Leu Asn Leu 275 280 285 Leu Asn Leu Asn Gly Ile Leu Tyr Leu Ile His Asn Pro Ser Asp Leu 290 295 300 Ser Leu Arg Arg Lys Glu Leu Trp Leu Ser Lys Leu Glu Asn Ser Asn 305 310 315 320 Ser Phe Lys Thr Leu Lys Val Leu Asp Lys Ala Asn Glu Val Ser Tyr 325 330 335 Pro Ser Tyr Ser Leu Asn Pro His Phe Ile Asp Ile Val Tyr Thr Tyr

340 345 350 Asn Arg Ser His Ile Lys His Ile Arg Phe Asn Met Ala Tyr Leu Asn 355 360 365 Ser Leu Leu Lys 370 (2) INFORMATION FOR SEQ ID NO:122: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 978 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...978 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:122: Met Lys Lys Arg Lys His Val Ser Lys Lys Val Phe Asn Val Ile Ile 1 5 10 15 Leu Phe Val Ala Val Phe Thr Leu Leu Val Val Ile His Lys Thr Leu 20 25 30 Ser Asn Gly Ile His Ile Gln Asn Leu Lys Ile Gly Lys Leu Gly Ile 35 40 45 Ser Glu Leu Tyr Leu Lys Leu Asn Asn Lys Leu Ser Leu Glu Val Glu 50 55 60 Arg Val Asp Leu Ser Ser Phe Phe His Gln Lys Pro Thr Lys Lys Arg 65 70 75 80 Leu Glu Val Ser Asp Leu Ile Lys Asn Ile Arg Tyr Gly Ile Trp Ala 85 90 95 Val Ser Tyr Phe Glu Lys Leu Lys Val Lys Glu Ile Ile Leu Asp Asp 100 105 110 Lys Asn Lys Ala Asn Ile Phe Phe Asp Gly Asn Lys Tyr Glu Leu Glu 115 120 125 Phe Pro Gly Ile Lys Gly Glu Phe Ser Leu Glu Asp Asp Lys Asn Ile 130 135 140 Lys Leu Lys Ile Ile Asn Leu Leu Phe Lys Asp Val Lys Val Gln Val 145 150 155 160 Asp Gly Asn Ala His Tyr Ser Pro Lys Ala Arg Lys Met Ala Phe Asn 165 170 175 Leu Ile Val Lys Pro Leu Val Glu Pro Ser Ala Ala Ile Tyr Leu Gln 180 185 190 Gly Leu Thr Asp Leu Lys Thr Ile Glu Leu Lys Ile Asn Thr Ser Pro 195 200 205 Met Lys Ser Leu Ala Phe Leu Lys Pro Leu Phe Gln Arg Gln Ser Gln 210 215 220 Lys Asn Leu Lys Thr Trp Ile Phe Asp Lys Ile Gln Phe Ala Ser Phe

225 230 235 240 Lys Ile Asp Asn Ala Leu Ile Lys Ala Asn Phe Thr Pro Ser Glu Phe 245 250 255 Ile Pro Ser Leu Leu Glu Asn Ser Val Val Lys Ala Thr Leu Ile Lys 260 265 270 Pro Ser Val Val Phe Asn Asp Gly Leu Ser Pro Ile Lys Met Asp Lys 275 280 285 Thr Glu Leu Ile Phe Lys Asn Lys Gln Leu Leu Ile Gln Pro Gln Lys 290 295 300 Ile Thr Tyr Glu Thr Met Glu Leu Thr Gly Ser Tyr Ala Thr Phe Ser 305 310 315 320 Asn Leu Leu Glu Ala Pro Lys Leu Glu Val Phe Leu Lys Thr Thr Pro 325 330 335 Asn Tyr Tyr Gly Asp Ser Ile Lys Asp Leu Leu Ser Ala Tyr Lys Val 340 345 350 Val Leu Pro Leu Asp Lys Ile Ser Met Pro Ser Ser Ala Asp Leu Lys 355 360 365 Leu Thr Leu Gln Phe Leu Lys Asn Thr Ala Pro Leu Phe Ser Val Gln 370 375 380 Gly Ser Val Asn Leu Gln Glu Gly Thr Phe Ser Leu Tyr Asn Ile Pro 385 390 395 400 Leu Tyr Thr Gln Ser Ala Gln Ile Asn Leu Asp Ile Ala Gln Glu Tyr 405 410 415 Gln Tyr Ile Tyr Ile Asp Thr Ile His Thr Arg Tyr Ala Asn Met Leu 420 425 430 Asp Leu Asp Ala Lys Ile Ala Leu Asp Leu Gly Gln Lys Asn Leu Ser 435 440 445 Leu Asp Ser Leu Val His Lys Ile Gln Val Asn Thr Asn Asn Asn Ile 450 455 460 Asn Met Arg Ser Tyr Asp Pro Asn Asn Thr Gln Glu Asp Pro Gln Thr 465 470 475 480 Asn Phe Thr Leu Asp Leu Lys Ser Leu His Ser Ile Ile Gln Glu Gly 485 490 495 Glu Asn Ser Glu Val Phe Arg Arg Lys Ile Ile Asp Thr Ile Lys Ala 500 505 510 Gln Ser Glu Asp Lys Phe Thr Lys Asp Val Phe Tyr Ala Thr Gly Asp 515 520 525 Thr Leu Lys Ser Leu Ser Leu Ser Phe Asp Phe Ser Asn Pro Asp His 530 535 540 Ile Gln Trp Ser Val Pro Gln Leu Leu Leu Glu Gly Glu Phe Lys Asp 545 550 555 560 Asn Ala Tyr Thr Phe Lys Ile Lys Asp Leu Lys Lys Ile Lys Pro Tyr 565 570 575 Ser Pro Ile Met Asp Tyr Ile Ala Leu Lys Asp Gly Ser Leu Glu Val 580 585 590 Ser Thr Ser Asp Phe Val Asn Ile Asp Phe Phe Ala Lys Asp Leu Lys 595 600 605 Ile Asn Leu Pro Ile Tyr Arg Ser Asp Gly Ser His Phe Asp Ser Phe 610 615 620 Ser Leu Phe Gly Ser Ile Asn Lys Asp Glu Ile Ser Val Tyr Thr Pro 625 630 635 640 Ser Lys Ser Ile Ser Ile Lys Val Lys Gly Asp Gln Lys Asp Ile Thr 645 650 655 Leu Asn Asn Ile Asp Leu Ser Ile Asp Asp Phe Leu Asp Ser Lys Met 660 665 670

Pro Ala Ile Ala Gly Leu Phe Ser Lys Glu Arg Lys Glu Lys Pro Ser 675 680 685 Ser Lys Glu Ile Gln Asp Glu Asp Val Phe Ile Ser Ala Lys Gln Arg 690 695 700 Tyr Glu Lys Ala His Lys Ile Ile Pro Ile Ser Thr Arg Ile His Ala 705 710 715 720 Lys Asp Val Val Leu Ile Tyr Lys Lys Met Pro Phe Pro Leu Glu Asn 725 730 735 Leu Asp Ile Val Ala Gln Asp Asp Arg Val Lys Ile Asp Gly Asn Tyr 740 745 750 Lys Asn Ala Met Ile Met Ala Asp Leu Val His Gly Ala Leu Tyr Leu 755 760 765 Lys Ala His Asn Phe Ser Gly Asp Tyr Ile Asn Thr Ile Leu Gln Lys 770 775 780 Asp Phe Val Glu Gly Gly Leu Phe Thr Leu Ile Gly Ala Leu Glu Asp 785 790 795 800 Gln Val Phe Asn Gly Glu Leu Lys Phe Gln Asn Thr Ser Leu Lys Asn 805 810 815 Phe Ala Leu Met Gln Asn Met Val Asn Leu Ile Asn Thr Ile Pro Ser 820 825 830 Leu Ile Val Phe Arg Asn Pro His Leu Gly Ala Asn Gly Tyr Gln Ile 835 840 845 Lys Thr Gly Ser Val Val Phe Gly Ile Thr Lys Glu Tyr Leu Gly Leu 850 855 860 Glu Lys Ile Asp Leu Val Gly Lys Thr Leu Asp Ile Ala Gly Asn Gly 865 870 875 880 Ile Ile Glu Leu Asp Lys Asn Lys Leu Asp Leu Asn Leu Glu Val Ser 885 890 895 Thr Ile Lys Ala Leu Ser Asn Val Leu Asn Lys Ile Pro Ile Val Gly 900 905 910 Tyr Leu Val Leu Gly Lys Gly Gly Lys Ile Thr Thr Asn Val Asn Val 915 920 925 Lys Gly Thr Leu Asp Lys Pro Lys Thr Gln Val Thr Leu Ala Ser Asp 930 935 940 Ile Ile Gln Ala Pro Phe Lys Ile Leu Arg Arg Ile Phe Thr Pro Ile 945 950 955 960 Asp Ile Ile Val Asp Glu Val Lys Lys Asn Ile Asp Ser Lys Arg Lys 965 970 975 Leu Lys (2) INFORMATION FOR SEQ ID NO:123: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 477 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori

(ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...477 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:123: Met Asn Thr Ile Ile Arg Tyr Ala Ser Leu Trp Gly Leu Cys Ile Thr 1 5 10 15 Leu Thr Leu Ala Gln Thr Pro Ser Lys Thr Pro Asp Glu Ile Lys Gln 20 25 30 Ile Leu Asn Asn Tyr Ser His Lys Asn Leu Lys Leu Ile Asp Pro Pro 35 40 45 Thr Ser Ser Leu Glu Ala Thr Pro Gly Phe Leu Pro Ser Pro Lys Glu 50 55 60 Thr Ala Thr Thr Ile Asn Gln Glu Ile Ala Lys Tyr His Glu Lys Ser 65 70 75 80 Asp Lys Ala Ala Leu Gly Leu Tyr Glu Leu Leu Lys Gly Ala Thr Thr 85 90 95 Asn Leu Ser Leu Gln Ala Gln Glu Leu Ser Val Lys Gln Ala Met Lys 100 105 110 Asn His Thr Ile Ala Lys Ala Met Phe Leu Pro Thr Leu Asn Ala Ser 115 120 125 Tyr Asn Phe Lys Asn Glu Ala Arg Asp Thr Pro Glu Tyr Lys His Tyr 130 135 140 Asn Thr Gln Gln Leu Gln Ala Gln Val Thr Leu Asn Val Phe Asn Gly 145 150 155 160 Phe Ser Asn Val Asn Asn Val Lys Glu Lys Ser Ala Thr Tyr Arg Ser 165 170 175 Thr Val Ala Asn Leu Glu Tyr Ser Arg Gln Ser Val Tyr Leu Gln Val 180 185 190 Val Gln Gln Tyr Tyr Glu Tyr Phe Asn Asn Leu Ala Arg Met Ile Ala 195 200 205 Leu Gln Lys Lys Leu Glu Gln Ile Gln Thr Asp Ile Lys Arg Val Thr 210 215 220 Lys Leu Tyr Asp Lys Gly Leu Thr Thr Ile Asp Asp Leu Gln Ser Leu 225 230 235 240 Lys Ala Gln Gly Asn Leu Ser Glu Tyr Asp Ile Leu Asp Met Gln Phe 245 250 255 Ala Leu Glu Gln Asn Arg Leu Thr Leu Glu Tyr Leu Thr Asn Leu Ser 260 265 270 Val Lys Asn Leu Lys Lys Thr Thr Ile Asp Ala Pro Asn Leu Gln Leu 275 280 285 Arg Glu Arg Gln Asp Leu Val Ser Leu Arg Glu Gln Ile Ser Ala Leu 290 295 300 Arg Tyr Gln Asn Lys Gln Leu Asn Tyr Tyr Pro Lys Ile Asp Val Phe 305 310 315 320 Asp Ser Trp Leu Phe Trp Ile Gln Lys Pro Ala Tyr Ala Thr Gly Arg 325 330 335 Phe Gly Asn Phe Tyr Pro Gly Gln Gln Asn Thr Ala Gly Val Thr Ala 340 345 350 Thr Leu Asn Ile Phe Asp Asp Ile Gly Leu Ser Leu Gln Lys Gln Ser 355 360 365 Ile Met Leu Gly Gln Leu Ala Asn Glu Lys Asn Leu Ala Tyr Lys Lys 370 375 380 Leu Glu Gln Glu Lys Asp Glu Gln Leu Tyr Arg Lys Ser Leu Asp Ile

385 390 395 400 Ala Arg Ala Lys Ile Glu Ser Ser Lys Ala Ser Leu Asp Ala Ala Asn 405 410 415 Leu Ser Phe Ala Asn Ile Lys Arg Lys Tyr Asp Ala Asn Leu Val Asp 420 425 430 Phe Thr Thr Tyr Leu Arg Gly Leu Thr Thr Arg Phe Asp Ala Glu Val 435 440 445 Ala Tyr Asn Leu Ala Leu Asn Asn Tyr Glu Val Gln Lys Ala Asn Tyr 450 455 460 Ile Phe Asn Ser Gly His Lys Ile Asp Asp Tyr Val His 465 470 475 (2) INFORMATION FOR SEQ ID NO:124: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 412 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...412 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:124: Met Leu Ser Phe Ile Ser Ala Phe Asp Lys Arg Gly Val Ser Ile Arg 1 5 10 15 Leu Leu Thr Ala Leu Leu Leu Leu Phe Ser Leu Gly Leu Ala Lys Asp 20 25 30 Leu Glu Ile Gln Thr Phe Val Ala Lys Tyr Leu Ser Lys Asn Gln Lys 35 40 45 Ile Gln Ala Leu Gln Glu Gln Ile Asp Ala Leu Asp Ser Gln Glu Lys 50 55 60 Val Val Ser Lys Trp Asp Asn Pro Ile Leu Tyr Leu Gly Tyr Asn Asn 65 70 75 80 Ala Asn Val Ser Asp Phe Phe Arg Leu Asp Ser Thr Leu Met Gln Asn 85 90 95 Met Ser Leu Gly Leu Ser Gln Lys Val Asp Leu Asn Gly Lys Lys Leu 100 105 110 Thr Gln Ser Lys Met Ile Asn Leu Glu Lys Gln Lys Lys Ile Leu Glu 115 120 125 Leu Lys Lys Thr Lys Gln Gln Leu Val Ile Asn Leu Met Ile Asn Gly 130 135 140 Ile Glu Asn Tyr Lys Asn Gln Gln Glu Ile Glu Leu Leu Asn Thr Ala 145 150 155 160 Ile Lys Asn Leu Glu Asn Thr Leu Tyr Gln Ala Asn His Ser Ser Ser 165 170 175 Pro Asp Leu Ile Ala Ile Ala Lys Leu Glu Ile Leu Lys Ser Leu Leu

180 185 190 Glu Ile Gln Lys Asn Asp Leu Glu Val Ala Leu Ser Ser Ser His Tyr 195 200 205 Ser Met Gly Glu Leu Thr Phe Lys Glu Asn Glu Ile Leu Ser Ile Ala 210 215 220 Pro Lys Asn Phe Glu Phe Asn Asn Glu Gln Glu Leu His Asn Ile Ser 225 230 235 240 Ala Thr Asn Tyr Asp Ile Ala Ile Ala Arg Leu Asp Glu Glu Lys Ala 245 250 255 Gln Lys Asp Ile Thr Leu Ala Lys Lys Ser Phe Leu Glu Asp Ile Asn 260 265 270 Val Thr Gly Val Tyr Tyr Phe Arg Ser Lys Gln Tyr Tyr Asn Tyr Asp 275 280 285 Met Phe Ser Val Ala Leu Ser Ile Pro Leu Pro Leu Tyr Gly Lys Gln 290 295 300 Ala Lys Leu Val Glu-Gln Lys Lys Lys Glu Ser Leu Ala Phe Lys Ser 305 310 315 320 Glu Val Glu Asn Ala Lys Asn Lys Thr Arg His Leu Ala Leu Lys Leu 325 330 335 Leu Lys Lys Leu Glu Thr Leu Gln Lys Asn Leu Glu Ser Ile Asn Lys 340 345 350 Ile Ile Lys Gln Asn Glu Lys Ile Ala Gln Ile Tyr Ala Leu Asp Leu 355 360 365 Lys Thr Asn Gly Asp Tyr Asn Ala Tyr Tyr Asn Ala Leu Asn Asp Lys 370 375 380 Ile Thr Ile Gln Ile Thr Gln Leu Glu Thr Leu Ser Ala Leu Asn Ser 385 390 395 400 Ala Tyr Leu Ser Leu Gln Asn Leu Lys Gly Leu Glu 405 410 (2) INFORMATION FOR SEQ ID NO:125: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 137 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...137 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:125: Met Arg Ile Val Arg Asn Leu Phe Leu Val Ser Phe Val Ala Tyr Ser 1 5 10 15 Ser Ala Phe Ala Ala Asp Leu Glu Thr Gly Thr Lys Asn Asp Lys Lys 20 25 30 Ser Gly Lys Lys Phe Tyr Lys Leu His Lys Asn His Gly Ser Glu Thr

35 40 45 Glu Thr Lys Asn Asp Lys Lys Leu Tyr Asp Phe Thr Lys Asn Ser Gly 50 55 60 Leu Glu Gly Val Asp Leu Glu Lys Ser Pro Asn Leu Lys Ser His Lys 65 70 75 80 Lys Ser Asp Lys Lys Phe Tyr Lys Gln Leu Ala Lys Asn Asn Ile Ala 85 90 95 Glu Gly Val Ser Met Pro Ile Val Asn Phe Asn Lys Ala Leu Ser Phe 100 105 110 Gly Pro Tyr Phe Glu Arg Thr Lys Ser Lys Lys Thr Gln Tyr Met Asp 115 120 125 Gly Gly Leu Met Met His Ile Arg Phe 130 135 (2) INFORMATION FOR SEQ ID NO:126: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 309 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...309 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:126: Leu Met Pro Gln Asn Gln Leu Val Ile Thr Ile Ile Asp Glu Ser Gly 1 5 10 15 Ser Lys Gln Leu Lys Phe Ser Lys Asn Leu Lys Arg Asn Leu Ile Ile 20 25 30 Ser Val Val Ile Leu Leu Leu Ile Val Gly Leu Gly Val Gly Phe Leu 35 40 45 Lys Phe Leu Ile Ala Lys Met Asp Thr Met Thr Ser Glu Arg Asn Ala 50 55 60 Val Leu Arg Asp Phe Arg Gly Leu Tyr Gln Lys Asn Tyr Ala Leu Ala 65 70 75 80 Lys Glu Ile Lys Asn Lys Arg Glu Glu Leu Phe Ile Val Gly Gln Lys 85 90 95 Ile Arg Gly Leu Glu Ser Leu Ile Glu Ile Lys Lys Gly Ala Asn Gly 100 105 110 Gly Gly His Leu Tyr Asp Glu Val Asp Leu Glu Asn Leu Ser Leu Asn 115 120 125 Gln Lys His Leu Ala Leu Met Leu Ile Pro Asn Gly Met Pro Leu Lys 130 135 140 Thr Tyr Ser Ala Ile Lys Pro Thr Lys Glu Arg Asn His Pro Ile Lys 145 150 155 160 Lys Ile Lys Gly Val Glu Ser Gly Ile Asp Phe Ile Ala Pro Leu Asn

165 170 175 Thr Pro Val Tyr Ala Ser Ala Asp Gly Ile Val Asp Phe Val Lys Thr 180 185 190 Arg Ser Asn Ala Gly Tyr Gly Asn Leu Val Arg Ile Glu His Ala Phe 195 200 205 Gly Phe Ser Ser Ile Tyr Thr His Leu Asp His Val Asn Val Gln Pro 210 215 220 Lys Ser Phe Ile Gln Lys Gly Gln Leu Ile Gly Tyr Ser Gly Lys Ser 225 230 235 240 Gly Asn Ser Gly Gly Glu Lys Leu His Tyr Glu Val Arg Phe Leu Gly 245 250 255 Lys Ile Leu Asp Ala Glu Lys Phe Leu Ala Trp Asp Leu Asp His Phe 260 265 270 Gln Ser Ala Leu Glu Glu Asn Lys Phe Ile Glu Trp Lys Asn Leu Phe 275 280 285 Trp Val Leu Glu Asp Ile Val Gln Leu Gln Glu His Val Asp Lys Asp 290 295 300 Thr Leu Lys Gly Gln 305 (2) INFORMATION FOR SEQ ID NO:127: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 332 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...332 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:127: Val Leu Tyr Phe Leu Thr Ser Leu Phe Ile Cys Ser Leu Ile Val Leu 1 5 10 15 Trp Ser Lys Lys Ser Met Leu Phe Val Asp Asn Ala Asn Lys Ile Gln 20 25 30 Gly Phe His His Ala Arg Thr Pro Arg Ala Gly Gly Leu Gly Ile Phe 35 40 45 Leu Ser Phe Ala Leu Ala Cys Tyr Leu Glu Pro Phe Glu Met Pro Phe 50 55 60 Lys Gly Pro Phe Val Phe Leu Gly Leu Ser Leu Val Phe Leu Ser Gly 65 70 75 80 Phe Leu Glu Asp Ile Asn Leu Ser Leu Ser Pro Lys Ile Arg Leu Ile 85 90 95 Leu Gln Ala Val Gly Val Val Cys Ile Ile Ser Ser Thr Pro Leu Val 100 105 110 Val Ser Asp Phe Ser Pro Leu Phe Ser Leu Pro Tyr Phe Ile Ala Phe

115 120 125 Leu Phe Ala Ile Phe Met Leu Val Gly Ile Ser Asn Ala Ile Asn Ile 130 135 140 Ile Asp Gly Phe Asn Gly Leu Ala Ser Gly Ile Cys Ala Ile Ala Leu 145 150 155 160 Leu Val Ile His Tyr Ile Asp Pro Ser Ser Leu Ser Cys Leu Leu Ala 165 170 175 Tyr Met Val Leu Gly Phe Met Val Leu Asn Phe Pro Ser Gly Lys Ile 180 185 190 Phe Leu Gly Asp Gly Gly Ala Tyr Phe Leu Gly Leu Val Cys Gly Ile 195 200 205 Ser Leu Leu His Leu Ser Leu Glu Gln Lys Ile Ser Val Phe Phe Gly 210 215 220 Leu Asn Leu Met Leu Tyr Pro Val Ile Glu Val Leu Phe Ser Ile Leu 225 230 235 240 Arg Arg Lys Ile Lys Arg Gln Lys Ala Thr Met Pro Asp Asn Leu His 245 250 255 Leu His Thr Leu Leu Phe Lys Phe Leu Gln Gln Arg Ser Phe Asn Tyr 260 265 270 Pro Asn Pro Leu Cys Ala Phe Ile Leu Ile Leu Cys Asn Leu Pro Phe 275 280 285 Ile Leu Ile Ser Val Leu Phe Arg Leu Asp Ala Tyr Ala Leu Ile Val 290 295 300 Ile Ser Leu Val Phe Ile Ala Cys Tyr Leu Ile Gly Tyr Ala Tyr Leu 305 310 315 320 Asn Arg Gln Val Cys Ala Leu Glu Lys Arg Ala Phe 325 330 (2) INFORMATION FOR SEQ ID NO:128: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 271 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1-...271 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:128: Met Asn Ile Phe Lys Arg Ile Ile Cys Val Thr Ala Ile Val Leu Gly 1 5 10 15 Phe Phe Asn Leu Leu Asp Ala Lys His His Lys Glu Lys Lys Glu Asp 20 25 30 His Lys Ile Thr Arg Glu Leu Lys Val Gly Ala Asn Pro Val Pro His 35 40 45 Ala Gln Ile Leu Gln Ser Val Val Asp Asp Leu Lys Glu Lys Gly Ile

50 55 60 Lys Leu Val Ile Val Ser Phe Thr Asp Tyr Val Leu Pro Asn Leu Ala 65 70 75 80 Leu Asn Asp Gly Ser Leu Asp Ala Asn Tyr Phe Gln His Arg Pro Tyr 85 90 95 Leu Asp Arg Phe Asn Leu Asp Arg Lys Met His Leu Val Gly Leu Ala 100 105 110 Asn Ile His Val Glu Pro Leu Arg Phe Tyr Ser Gln Lys Ile Thr Asp 115 120 125 Ile Lys Asn Leu Lys Lys Gly Ser Val Ile Ala Val Pro Asn Asp Pro 130 135 140 Ala Asn Gln Gly Arg Ala Leu Ile Leu Leu His Lys Gln Gly Leu Ile 145 150 155 160 Ala Leu Lys Asp Pro Ser Asn Leu Tyr Ala Thr Glu Phe Asp Ile Val 165 170 175 Lys Asn Pro Tyr Asn Ile Lys Ile Lys Pro Leu Glu Ala Ala Leu Leu 180 185 190 Pro Lys Val Leu Gly Asp Val Asp Gly Ala Ile Ile Thr Gly Asn Tyr 195 200 205 Ala Leu Gln Ala Lys Leu Thr Gly Ala Leu Phe Ser Glu Asp Lys Asp 210 215 220 Ser Pro Tyr Ala Asn Leu Val Ala Ser Arg Glu Asp Asn Ala Gln Asp 225 230 235 240 Glu Ala Ile Lys Ala Leu Ile Glu Ala Leu Gln Ser Glu Lys Thr Arg 245 250 255 Lys Phe Ile Leu Asp Thr Tyr Lys Gly Ala Ile Ile Pro Ala Phe 260 265 270 (2) INFORMATION FOR SEQ ID NO:129: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 316 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...316 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:129: Met Gln Glu Phe Ser Leu Trp Cys Asp Phe Ile Glu Arg Asp Phe Leu 1 5 10 15 Glu Asn Asp Phe Leu Lys Leu Ile Asn Lys Gly Ala Ile Cys Gly Ala 20 25 30 Thr Ser Asn Pro Ser Leu Phe Cys Glu Ala Ile Thr Lys Ser Ala Phe 35 40 45 Tyr Gln Asp Glu Ile Ala Lys Leu Lys Gly Lys Lys Ala Lys Glu Ile

50 55 60 Tyr Glu Thr Leu Ala Leu Lys Asp Ile Leu Gln Ala Ser Ser Ala Leu 65 70 75 80 Met Pro Leu Tyr Glu Lys Asp Pro Asn Asn Gly Tyr Ile Ser Leu Glu 85 90 95 Ile Asp Pro Phe Leu Glu Asp Asp Ala Ile Lys Ser Ile Asp Glu Ala 100 105 110 Lys Arg Leu Phe Lys Thr Leu Asn Arg Pro Asn Val Met Ile Lys Val 115 120 125 Pro Ala Ser Glu Ser Ala Phe Glu Val Ile Ser Ala Leu Ala Gln Ala 130 135 140 Ser Ile Pro Ile Asn Val Thr Leu Val Phe Ser Pro Lys Ile Ala Gly 145 150 155 160 Glu Ile Ala Gln Ile Leu Ala Lys Glu Ala Arg Lys Arg Ala Val Ile 165 170 175 Ser Val Phe Val Ser Arg Phe Asp Lys Glu Ile Asp Pro Leu Val Pro 180 185 190 Gln Asn Leu Gln Ala Gln Ser Gly Ile Met Asn Ala Thr Glu Cys Tyr 195 200 205 Tyr Gln Ile Asn Gln His Ala Asn Lys Leu Ile Ser Thr Leu Phe Ala 210 215 220 Ser Thr Gly Val Lys Ser Asn Ser Leu Ala Lys Asp Tyr Tyr Ile Lys 225 230 235 240 Ala Leu Cys Phe Lys Asn Ser Ile Asn Thr Ala Pro Leu Asp Ala Leu 245 250 255 Asn Ala Tyr Leu Leu Asp Pro Asn Thr Glu Cys Gln Thr Pro Leu Lys 260 265 270 Ile Thr Glu Ile Glu Ala Phe Lys Lys Glu Leu Lys Thr His Asn Ile 275 280 285 Asp Leu Glu Asn Thr Ala Gln Lys Leu Leu Lys Glu Gly Leu Ile Ala 290 295 300 Phe Lys Gln Ser Phe Glu Lys Leu Leu Ser Ser Phe 305 310 315 (2) INFORMATION FOR SEQ ID NO:130: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 260 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...260 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:130: Met Lys Thr Asn Gly His Phe Lys Asp Phe Ala Trp Lys Lys Cys Phe

1 5 10 15 Leu Gly Ala Ser Val Val Ala Leu Leu Val Gly Cys Ser Pro His Ile 20 25 30 Ile Glu Thr Asn Glu Val Ala Leu Lys Leu Asn Tyr His Pro Ala Ser 35 40 45 Glu Lys Val Gln Ala Leu Asp Glu Lys Ile Leu Leu Leu Arg Pro Ala 50 55 60 Phe Gln Tyr Ser Asp Asn Ile Ala Lys Glu Tyr Glu Asn Lys Phe Lys 65 70 75 80 Asn Gln Thr Thr Leu Lys Val Glu Glu Ile Leu Gln Asn Gln Gly Tyr 85 90 95 Lys Val Ile Asn Val Asp Ser Ser Asp Lys Asp Asp Phe Ser Phe Ala 100 105 110 Gln Lys Lys Glu Gly Tyr Leu Ala Val Ala Met Asn Gly Glu Ile Val 115 120 125 Leu Arg Pro Asp Pro Lys Arg Thr Ile Gln Lys Lys Ser Glu Pro Gly 130 135 140 Leu Leu Phe Ser Thr Gly Leu Asp Lys Met Glu Arg Val Leu Ile Pro 145 150 155 160 Ala Gly Phe Val Lys Val Thr Ile Leu Glu Pro Met Ser Gly Glu Ser 165 170 175 Leu Asp Ser Phe Thr Met Asp Leu Ser Glu Leu Asp Ile Gln Glu Lys 180 185 190 Phe Leu Lys Thr Thr His Ser Ser His Ser Gly Gly Leu Val Ser Thr 195 200 205 Met Val Lys Gly Thr Asp Asn Ser Asn Asp Ala Ile Lys Ser Ala Leu 210 215 220 Asn Lys Ile Phe Ala Ser Ile Met Gln Glu Met Asp Lys Lys Leu Thr 225 230 235 240 Gln Arg Asn Leu Glu Ser Tyr Gln Lys Asp Ala Lys Glu Leu Lys Asn 245 250 255 Lys Arg Asn Arg 260 (2) INFORMATION FOR SEQ ID NO:131: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1382 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...1382 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:131: Leu Asn Phe Asn Asn Leu Thr Ala Asn Gly Ala Leu Asn Phe Asn Gly

1 5 10 15 Tyr Ala Pro Ser Leu Thr Lys Ala Leu Met Asn Val Ser Gly Gln Phe 20 25 30 Val Leu Gly Asn Asn Gly Asp Ile Asn Leu Ser Asp Ile Asn Ile Phe 35 40 45 Asp Asn Ile Thr Lys Ser Val Thr Tyr Asn Ile Leu Asn Ala Gln Lys 50 55 60 Gly Ile Thr Gly Ile Ser Gly Ala Asn Gly Tyr Glu Lys Ile Leu Phe 65 70 75 80 Tyr Gly Met Lys Ile Gln Asn Ala Thr Tyr Ser Asp Asn Asn Asn Ile 85 90 95 Gln Thr Trp Ser Phe Ile Asn Pro Leu Asn Ser Ser Gln Ile Ile Gln 100 105 110 Glu Ser Ile Lys Asn Gly Asp Leu Thr Ile Glu Val Leu Asn Asn Pro 115 120 125 Asn Ser Ala Ser Asn Thr Ile Phe Asn Ile Ala Pro Glu Leu Tyr Asn 130 135 140 Tyr Gln Asp Ser Lys Gln Asn Pro Thr Gly Tyr Ser Tyr Asp Tyr Ser 145 150 155 160 Asp Asn Gln Ala Gly Thr Tyr Tyr Leu Thr Ser Asn Ile Lys Gly Leu 165 170 175 Phe Thr Pro Lys Gly Ser Gln Thr Pro Gln Thr Pro Gly Thr Tyr Ser 180 185 190 Pro Phe Asn Gln Pro Leu Asn Ser Leu Asn Ile Tyr Asn Lys Gly Phe 195 200 205 Ser Ser Glu Asn Leu Lys Thr Leu Leu Gly Ile Leu Ser Gln Asn Ser 210 215 220 Ala Thr Leu Lys Glu Met Ile Glu Ser Asn Gln Leu Asp Asn Ile Thr 225 230 235 240 Asn Ile Asn Glu Val Leu Gln Leu Leu Asp Lys Ile Lys Ile Thr Gln 245 250 255 Ala Gln Lys Gln Ala Leu Leu Glu Thr Ile Asn His Leu Thr Asp Asn 260 265 270 Ile Asn Gln Thr Phe Asn Asn Gly Asn Leu Val Ile Gly Ala Thr Gln 275 280 285 Asp Asn Val Thr Asn Ser Thr Ser Ser Ile Trp Phe Gly Gly Asn Gly 290 295 300 Tyr Ser Ser Pro Cys Ala Leu Asp Ser Ala Thr Cys Ser Ser Phe Arg 305 310 315 320 Asn Thr Tyr Leu Gly Gln Leu Leu Gly Ser Thr Ser Pro Tyr Leu Gly 325 330 335 Tyr Ile Asn Ala Asp Phe Lys Ala Lys Ser Ile Tyr Ile Thr Gly Thr 340 345 350 Ile Gly Ser Ser Asn Ala Phe Glu Ser Gly Gly Ser Ala Asp Val Thr 355 360 365 Phe Gln Ser Ala Asn Asn Leu Val Leu Asn Lys Ala Asn Ile Glu Ala 370 375 380 Gln Ala Thr Asp Asn Ile Phe Asn Leu Leu Gly Gln Glu Gly Ile Asp 385 390 395 400 Lys Ile Phe Asn Gln Gly Asn Leu Ala Asn Val Leu Ser Gln Met Ala 405 410 415 Met Glu Lys Ile Lys Gln Ala Gly Gly Leu Gly Asn Phe Ile Glu Asn 420 425 430 Ala Leu Ser Pro Leu Ser Lys Glu Leu Pro Ala Ser Leu Gln Asp Glu 435 440 445

Thr Leu Gly Gln Leu Ile Gly Gln Asn Asn Leu Asp Asp Leu Leu Asn 450 455 460 Asn Ser Gly Val Met Asn Glu Ile Gln Asn Ile Ile Ser Gln Lys Leu 465 470 475 480 Ser Ile Phe Gly Asn Phe Val Thr Pro Ser Ile Ile Glu Asn Tyr Leu 485 490 495 Ala Lys Gln Ser Leu Lys Ser Met Leu Asp Asp Lys Gly Leu Leu Asn 500 505 510 Phe Ile Gly Gly Tyr Ile Asp Ala Ser Glu Leu Ser Ser Ile Leu Gly 515 520 525 Val Ile Leu Lys Asp Ile Thr Asn Pro Pro Thr Ser Leu Gln Lys Asp 530 535 540 Ile Gly Val Val Ala Asn Asp Leu Leu Asn Glu Phe Leu Gly Gln Asp 545 550 555 560 Val Val Lys Lys Leu Glu Ser Gln Gly Leu Val Ser Asn Ile Ile Asn 565 570 575 Asn Val Ile Ser Gln Gly Gly Leu Ser Gly Val Tyr Asn Gln Gly Leu 580 585 590 Gly Ser Val Leu Pro Pro Ser Leu Gln Asn Ala Leu Lys Glu Asn Asp 595 600 605 Leu Gly Thr Leu Leu Ser Pro Arg Gly Leu His Asp Phe Trp Gln Lys 610 615 620 Gly Tyr Phe Asn Phe Leu Ser Asn Gly Tyr Val Phe Val Asn Asn Ser 625 630 635 640 Ser Phe Ser Asn Ala Thr Gly Gly Ser Leu Asn Phe Val Ala Asn Lys 645 650 655 Ser Ile Ile Phe Asn Gly Asp Asn Thr Ile Asp Phe Ser Lys Tyr Gln 660 665 670 Gly Ala Leu Ile Phe Ala Ser Asn Gly Val Ser Asn Ile Asn Ile Thr 675 680 685 Thr Leu Asn Ala Thr Asn Gly Leu Ser Leu Asn Ala Gly Leu Asn Asn 690 695 700 Val Ser Val Gln Lys Gly Glu Ile Cys Ile Asn Leu Ala Asn Cys Pro 705 710 715 720 Thr Thr Lys Asn Ser Ser Pro Ala Asn Ser Ser Val Thr Pro Thr Asn 725 730 735 Glu Ser Leu Ser Val His Ala Asn Asn Phe Thr Phe Leu Gly Thr Ile 740 745 750 Ile Ser Asn Gly Ala Ile Asp Leu Ser Gln Val Thr Asn Asn Ser Val 755 760 765 Ile Gly Thr Leu Asn Leu Asn Glu Asn Ala Thr Leu Gln Ala Asn Asn 770 775 780 Leu Thr Ile Thr Asn Ala Phe Asn Asn Ala Ser Asn Ser Thr Ala Asn 785 790 795 800 Ile Asp Gly Asn Phe Thr Leu Asn Gln Gln Ala Thr Leu Ser Thr Asn 805 810 815 Ala Ser Gly Leu Asn Val Met Gly Asn Phe Asn Ser Tyr Gly Asp Leu 820 825 830 Val Phe Asn Leu Ser His Ser Val Ser His Ala Ile Ile Asn Thr Gln 835 840 845 Gly Thr Ala Thr Ile Met Ala Asn Asn Asn Pro Leu Ile Gln Phe Asn 850 855 860 Ala Ser Ser Lys Glu Val Gly Thr Tyr Thr Leu Ile Asp Ser Ala Lys 865 870 875 880 Ala Ile Tyr Tyr Gly Tyr Asn Asn Gln Ile Thr Gly Gly Ser Ser Leu

885 890 895 Asp Asn Tyr Leu Lys Leu Tyr Ala Leu Ile Asp Ile Asn Gly Lys His 900 905 910 Met Val Met Thr Asp Asn Gly Leu Thr Tyr Asn Gly Gln Ala Val Ser 915 920 925 Val Lys Asp Gly Gly Leu Val Val Gly Phe Lys Asp Ser Gln Asn Gln 930 935 940 Tyr Ile Tyr Thr Ser Ile Leu Tyr Asn Lys Val Lys Ile Ala Val Ser 945 950 955 960 Asn Asp Pro Ile Asn Asn Pro Gln Ala Pro Thr Leu Lys Gln Tyr Ile 965 970 975 Ala Gln Ile Gln Gly Val Gln Ser Val Asp Ser Ile Asp Gln Ala Gly 980 985 990 Gly Asn Gln Ala Ile Asn Trp Leu Asn Lys Ile Phe Glu Thr Lys Gly 995 1000 1005 Ser Pro Leu Phe Ala Pro Tyr Tyr Leu Glu Ser His Ser Thr Lys Asp 1010 1015 1020 Leu Thr Thr Ile Ala Gly Asp Ile Ala Asn Thr Leu Glu Val Ile Ala 1025 1030 1035 1040 Asn Pro Asn Phe Lys Asn Asp Ala Thr Asn Ile Leu Gln Ile Asn Thr 1045 1050 1055 Tyr Thr Gln Gln Met Ser Arg Leu Ala Lys Leu Ser Asp Thr Ser Thr 1060 1065 1070 Phe Ala Arg Ser Asp Phe Leu Glu Arg Leu Glu Ala Leu Lys Asn Lys 1075 1080 1085 Arg Phe Ala Asp Ala Ile Pro Asn Ala Met Asp Val Ile Leu Lys Tyr 1090 1095 1100 Ser Gln Arg Asn Arg Val Lys Asn Asn Val Trp Ala Thr Gly Val Gly 1105 1110 1115 1120 Gly Ala Ser Phe Ile Ser Gly Gly Thr Gly Thr Leu Tyr Gly Ile Asn 1125 1130 1135 Val Gly Tyr Asp Arg Phe Ile Lys Gly Val Ile Val Gly Gly Tyr Ala 1140 1145 1150 Ala Tyr Gly Tyr Ser Gly Phe His Ala Asn Ile Thr Gln Ser Gly Ser 1155 1160 1165 Ser Asn Val Asn Val Gly Val Tyr Ser Arg Ala Phe Ile Lys Arg Ser 1170 1175 1180 Glu Leu Thr Met Ser Leu Asn Glu Thr Trp Gly Tyr Asn Lys Thr Phe 1185 1190 1195 1200 Ile Asn Ser Tyr Asp Pro Leu Leu Ser Ile Ile Asn Gln Ser Tyr Arg 1205 1210 1215 Tyr Asp Thr Trp Thr Thr Asp Ala Lys Ile Asn Tyr Gly Tyr Asp Phe 1220 1225 1230 Met Phe Lys Asp Lys Ser Val Ile Phe Lys Pro Gln Val Gly Leu Ser 1235 1240 1245 Tyr Tyr Tyr Ile Gly Leu Ser Gly Leu Arg Gly Ile Met Asp Asp Pro 1250 1255 1260 Ile Tyr Asn Gln Phe Arg Ala Asn Ala Asp Pro Asn Lys Lys Ser Val 1265 1270 1275 1280 Leu Thr Ile Asn Phe Ala Leu Glu Ser Arg His Tyr Phe Asn Lys Asn 1285 1290 1295 Ser Tyr Tyr Phe Val Ile Ala Asp Val Gly Arg Asp Leu Phe Ile Asn 1300 1305 1310 Ser Met Gly Asp Lys Met Val Arg Phe Ile Gly Asn Asn Thr Leu Ser 1315 1320 1325

Tyr Arg Asp Gly Gly Arg Tyr Asn Thr Phe Ala Ser Ile Ile Thr Gly 1330 1335 1340 Gly Glu Ile Arg Leu Phe Lys Thr Phe Tyr Val Asn Ala Gly Ile Gly 1345 1350 1355 1360 Ala Arg Phe Gly Leu Asp Tyr Lys Asp Ile Asn Ile Thr Gly Asn Ile 1365 1370 1375 Gly Met Arg Tyr Ala Phe 1380 (2) INFORMATION FOR SEQ ID NO:132: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 262 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...262 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:132: Met Lys Lys Ile Gly Leu Ser Leu Cys Leu Val Leu Ser Leu Gly Phe 1 5 10 15 Leu Lys Ala His Glu Val Ser Ala Glu Glu Ile Ala Asp Ile Phe Tyr 20 25 30 Lys Leu Asn Ala Lys Glu Pro Lys Met Lys Ile Asn His Thr Lys Gly 35 40 45 Phe Cys Ala Lys Gly Val Phe Leu Pro Asn Pro Gln Ala Arg Glu Asp 50 55 60 Leu Glu Val Pro Leu Leu Asn Glu Lys Glu Ile Pro Ala Ser Val Arg 65 70 75 80 Tyr Ser Leu Gly Gly Val Ala Met Asp Asp Lys Ser Lys Val Arg Gly 85 90 95 Met Ala Leu Lys Leu Glu Asn Gln Asn Ala Ser Trp Thr Met Val Met 100 105 110 Leu Asn Thr Glu Ile Asn Phe Ala Lys Asn Pro Glu Glu Phe Ala Gln 115 120 125 Phe Phe Glu Met Arg Leu Pro Lys Asn Gly Lys Val Asp Glu Ala Arg 130 135 140 Ile Lys Lys Leu Tyr Glu Glu Val Pro Ser Tyr Arg Asn Phe Ala Ala 145 150 155 160 Tyr Met Lys Thr Ile Gly Ile Ser Ser Ser Val Ala Asn Thr Pro Tyr 165 170 175 Tyr Ser Val His Ala Phe Lys Phe Lys Asp Lys Lys Glu Lys Leu Leu 180 185 190 Pro Ala Arg Trp Lys Phe Val Pro Lys Glu Gly Val Lys Tyr Leu Asn 195 200 205

Pro Gln Glu Leu Lys Gln Lys Asp Ser Asn Tyr Leu Leu Ser Ser Phe 210 215 220 Gln Gln His Leu Lys Asn Lys Pro Ile Glu Tyr Gln Met Tyr Leu Val 225 230 235 240 Phe Ala Asn Gln Asn Asp Ala Thr Asn Asp Thr Thr Ala Leu Trp Lys 245 250 255 Gly Ser Ile Arg Asn Tyr 260 (2) INFORMATION FOR SEQ ID NO:133: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 246 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...246 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:133: Met Lys Gln Phe Lys Lys Lys Pro Lys Lys Ile Lys Arg Ser His Gln 1 5 10 15 Asn Gln Lys Thr Ile Leu Lys Arg Pro Leu Trp Leu Met Pro Leu Leu 20 25 30 Ile Gly Gly Phe Ala Ser Gly Val Tyr Ala Asp Gly Thr Asp Ile Leu 35 40 45 Gly Leu Ser Trp Gly Glu Lys Ser Gln Lys Val Cys Val His Arg Pro 50 55 60 Trp Tyr Ala Ile Trp Ser Cys Asp Lys Trp Glu Glu Lys Thr Gln Gln 65 70 75 80 Phe Thr Gly Asn Gln Leu Ile Thr Lys Thr Trp Ala Gly Gly Asn Ala 85 90 95 Ala Asn Tyr Tyr His Ser Gln Asn Asn Gln Asp Ile Thr Ala Asn Leu 100 105 110 Lys Asn Asp Asn Gly Thr Tyr Phe Leu Ser Gly Leu Tyr Asn Tyr Thr 115 120 125 Gly Gly Glu Tyr Asn Gly Gly Asn Leu Asp Ile Glu Leu Gly Ser Asn 130 135 140 Ala Thr Phe Asn Leu Gly Ala Ser Ser Gly Asn Ser Phe Thr Ser Trp 145 150 155 160 Tyr Pro Asn Gly His Thr Asp Val Thr Phe Ser Ala Gly Thr Ile Asn 165 170 175 Val Asn Asn Ser Val Glu Val Gly Asn Arg Val Gly Ser Gly Ala Gly 180 185 190 Thr His Thr Gly Thr Ala Thr Leu Asn Leu Asn Ala Asn Lys Val Thr 195 200 205

Ile Asn Ser Asn Ile Ser Ala Tyr Lys Thr Ser Gln Val Asn Val Gly 210 215 220 Asn Ala Asn Ser Val Ile Thr Ile Asn Ser Val Ser Leu Asn Gly Glu 225 230 235 240 Tyr Leu Gln Phe Phe Ser 245 (2) INFORMATION FOR SEQ ID NO:134: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 245 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...245 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:134: Met Ile Lys Lys Thr Leu Ala Ser Val Leu Leu Gly Leu Ser Leu Met 1 5 10 15 Ser Val Leu Asn Ala Lys Glu Cys Val Ser Pro Ile Thr Arg Ser Val 20 25 30 Lys Tyr His Gln Gln Ser Ala Glu Ile Arg Ala Leu Gln Leu Gln Ser 35 40 45 Tyr Lys Met Ala Lys Met Ala Leu Asp Asn Asn Leu Lys Leu Val Lys 50 55 60 Asp Lys Lys Pro Ala Val Ile Leu Asp Leu Asp Glu Thr Val Leu Asn 65 70 75 80 Thr Phe Asp Tyr Ala Gly Tyr Leu Val Lys Asn Cys Ile Lys Tyr Thr 85 90 95 Pro Glu Thr Trp Asp Lys Phe Glu Lys Glu Gly Ser Leu Thr Leu Ile 100 105 110 Pro Gly Ala Leu Asp Phe Leu Glu Tyr Ala Asn Ser Lys Gly Val Lys 115 120 125 Ile Phe Tyr Ile Ser Asn Arg Thr Gln Lys Asn Lys Ala Phe Thr Leu 130 135 140 Lys Thr Leu Lys Ser Phe Lys Leu Pro Gln Val Ser Glu Glu Ser Val 145 150 155 160 Leu Leu Lys Glu Lys Gly Lys Pro Lys Ala Val Arg Arg Glu Leu Val 165 170 175 Ala Lys Asp Tyr Ala Ile Val Leu Gln Val Gly Asp Thr Leu His Asp 180 185 190 Phe Asp Ala Ile Phe Ala Lys Asp Ala Lys Asn Ser Gln Glu Gln Gln 195 200 205 Ala Lys Val Leu Gln Asn Ala Gln Lys Phe Gly Thr Glu Trp Ile Ile 210 215 220

Leu Pro Asn Ser Leu Tyr Gly Thr Trp Glu Asp Gly Pro Ile Lys Ala 225 230 235 240 Trp Gln Asn Lys Lys 245 (2) INFORMATION FOR SEQ ID NO:135: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 288 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...288 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:135: Leu Trp Cys Leu Lys Thr Pro Ile Ile Gly His Gly Met Lys Lys Lys 1 5 10 15 Ala Lys Val Phe Trp Cys Cys Phe Lys Met Ile Arg Trp Leu Tyr Leu 20 25 30 Ala Val Phe Phe Leu Leu Ser Val Ser Asp Ala Lys Glu Ile Ala Met 35 40 45 Gln Arg Phe Asp Lys Gln Asn His Lys Ile Phe Glu Ile Leu Ala Asp 50 55 60 Lys Val Ser Ala Lys Asp Asn Val Ile Thr Ala Ser Gly Asn Ala Ile 65 70 75 80 Leu Leu Asn Tyr Asp Val Tyr Ile Leu Ala Asp Lys Val Arg Tyr Asp 85 90 95 Thr Lys Thr Lys Glu Ala Leu Leu Glu Gly Asn Ile Lys Val Tyr Arg 100 105 110 Gly Glu Gly Leu Leu Val Lys Thr Asp Tyr Val Lys Leu Ser Leu Asn 115 120 125 Glu Lys Tyr Glu Ile Ile Phe Pro Phe Tyr Val Gln Asp Ser Val Ser 130 135 140 Gly Ile Trp Val Ser Ala Asp Ile Ala Ser Gly Lys Asp Gln Lys Tyr 145 150 155 160 Lys Ile Lys Asn Met Ser Ala Ser Gly Cys Ser Ile Asp Asn Pro Ile 165 170 175 Trp His Val Asn Ala Thr Ser Gly Ser Phe Asn Met Gln Lys Ser His 180 185 190 Leu Ser Met Trp Asn Pro Lys Ile Tyr Val Gly Asp Ile Pro Val Leu 195 200 205 Tyr Leu Pro Tyr Ile Phe Met Ser Thr Ser Asn Lys Arg Thr Thr Gly 210 215 220 Phe Leu Tyr Pro Glu Phe Gly Thr Ser Asn Leu Asp Gly Phe Ile Tyr 225 230 235 240

Leu Gln Pro Phe Tyr Leu Ala Pro Lys Asn Ser Trp Asp Met Thr Phe 245 250 255 Thr Pro Gln Ile Arg Tyr Lys Arg Gly Phe Gly Leu Asn Phe Glu Ala 260 265 270 Arg Tyr Ile Asn Ser Lys Thr Gln Val Phe Ile Gln Cys Ala Leu Phe 275 280 285 (2) INFORMATION FOR SEQ ID NO:136: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 128 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...128 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:136: Leu Met Phe Lys Lys Met Cys Leu Ser Leu Leu Met Ile Ser Gly Val 1 5 10 15 Cys Val Gly Ala Lys Asp Leu Asp Phe Lys Leu Asp Tyr Arg Ala Thr 20 25 30 Gly Gly Lys Phe Met Gly Lys Met Thr Asp Ser Ser Leu Leu Ser Ile 35 40 45 Thr Ser Met Asn Asp Glu Pro Val Val Ile Lys Asn Leu Ile Val Asn 50 55 60 Arg Gly Asn Ser Cys Glu Ala Thr Lys Lys Val Glu Pro Lys Phe Gly 65 70 75 80 Asp Lys Phe Lys Lys Glu Lys Leu Phe Asp His Glu Leu Lys Tyr Ser 85 90 95 Gln Gln Ile Phe Tyr Arg Leu Asp Cys Lys Pro Asn Gln Leu Leu Glu 100 105 110 Val Lys Ile Ile Thr Asp Lys Gly Glu Tyr Tyr His Lys Phe Ser Lys 115 120 125 (2) INFORMATION FOR SEQ ID NO:137: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 169 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES

(vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...169 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:137: Met Gln Ala Leu Lys Ser Leu Leu Glu Val Ile Thr Lys Leu Gln Asn 1 5 10 15 Leu Gly Gly Tyr Leu Met His Ile Ala Ile Phe Ile Ile Phe Ile Trp 20 25 30 Ile Gly Gly Leu Lys Phe Val Pro Tyr Glu Ala Glu Gly Ile Ala Pro 35 40 45 Phe Val Ala Asn Ser Pro Phe Phe Ser Phe Met Tyr Lys Phe Glu Lys 50 55 60 Pro Ala Tyr Lys Gln His Lys Met Ser Glu Ser Gln Ser Met Gln Glu 65 70 75 80 Glu Met Gln Asp Asn Pro Lys Ile Val Glu Asn Lys Glu Trp His Lys 85 90 95 Glu Asn Arg Thr Tyr Leu Val Ala Glu Gly Leu Gly Ile Thr Ile Met 100 105 110 Ile Leu Gly Ile Leu Val Leu Leu Gly Leu Trp Met Pro Leu Met Gly 115 120 125 Val Val Gly Gly Leu Leu Val Ala Gly Met Thr Ile Thr Thr Leu Phe 130 135 140 Phe Phe Ile His Asn Ala Arg Ser Val Cys Gln Ser Ala Phe Pro Met 145 150 155 160 Ala Phe Trp Gly Trp Lys Ala Ser Gly 165 (2) INFORMATION FOR SEQ ID NO:138: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 487 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...487 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:138: Met Ile Glu Trp Met Gln Asn His Arg Lys Tyr Leu Val Val Thr Ile 1 5 10 15 Trp Ile Ser Thr Ile Ala Phe Ile Ala Ala Gly Met Ile Gly Trp Gly

20 25 30 Gln Tyr Ser Phe Ser Leu Asp Ser Asp Ser Ala Ala Lys Val Gly Gln 35 40 45 Ile Lys Ile Ser Gln Glu Glu Leu Ala Gln Glu Tyr Arg Arg Leu Lys 50 55 60 Asp Ala Tyr Ala Glu Ser Ile Pro Asp Phe Lys Glu Leu Thr Glu Asp 65 70 75 80 Gln Ile Lys Ala Met His Leu Glu Lys Ser Ala Leu Asp Ser Leu Ile 85 90 95 Asn Gln Ala Leu Leu Arg Asn Phe Ala Leu Asp Leu Gly Leu Gly Ala 100 105 110 Thr Lys Gln Glu Val Ala Lys Glu Ile Arg Lys Thr Asn Val Phe Gln 115 120 125 Lys Asp Gly Val Phe Asp Glu Glu Leu Tyr Lys Asn Ile Leu Lys Gln 130 135 140 Ser His Tyr Arg Pro Lys His Phe Glu Glu Ser Val Glu Arg Leu Leu 145 150 155 160 Ile Leu Gln Lys Ile Ser Ala Leu Phe Pro Lys Thr Thr Thr Pro Leu 165 170 175 Glu Gln Ser Ser Leu Ser Leu Trp Ala Lys Leu Gln Asp Lys Leu Asp 180 185 190 Ile Leu Ile Leu Asn Pro Asn Asp Val Lys Ile Ser Leu Asn Glu Glu 195 200 205 Glu Met Lys Lys'Tyr Tyr Glu Asn His Arg Lys Asp Phe Lys Lys Pro 210 215 220 Thr Ser Phe Lys Thr Arg Ser Leu Tyr Phe Asp Ala Ser Leu Glu Lys 225 230 235 240 Thr Asp Leu Lys Glu Leu Glu Glu Tyr Tyr His Lys Asn Lys Val Ser 245 250 255 Tyr Leu Asp Lys Glu Gly Lys Leu Gln Asp Phe Lys Ser Val Gln Glu 260 265 270 Gln Val Lys His Asp Leu Asn Met Gln Lys Ala Asn Glu Lys Ala Leu 275 280 285 Arg Ser Tyr Ile Ala Leu Lys Lys Gly Asn Ala Gln Asn Tyr Thr Thr 290 295 300 Gln Asp Phe Glu Lys Asn Asn Ser Pro Tyr Thr Ala Glu Ile Thr Gln 305 310 315 320 Lys Leu Thr Ala Leu Lys Pro Leu Glu Val Leu Lys Pro Glu Pro Phe 325 330 335 Lys Asp Gly Phe Ile Val Val Gln Leu Val Ser Gln Ile Lys Asp Glu 340 345 350 Leu Gln Asn Phe Asp Glu Ala Lys Ser Ala Leu Lys Thr Arg Leu Thr 355 360 365 Gln Glu Lys Thr Leu Met Ala Leu Gln Thr Leu Ala Lys Glu Lys Leu 370 375 380 Lys Asp Phe Lys Gly Lys Ser Val Gly Tyr Val Ser Pro Asn Phe Gly 385 390 395 400 Gly Thr Ile Ser Glu Leu Asn Gln Glu Glu Ser Ala Lys Phe Ile Asn 405 410 415 Thr Leu Phe Asn Arg Gln Glu Lys Lys Gly Phe Val Thr Ile Gly Asn 420 425 430 Lys Val Val Leu Tyr Gln Ile Thr Glu Gln Asn Phe Asn His Pro Phe 435 440 445 Ser Ala Glu Glu Asn Gln Tyr Met Gln Arg Leu Val Asn Asn Thr Lys 450 455 460

Thr Asp Phe Phe Asp Lys Ala Leu Ile Glu Glu Leu Lys Lys Arg Tyr 465 470 475 480 Lys Ile Val Lys Tyr Ile Gln 485 (2) INFORMATION FOR SEQ ID NO:139: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 142 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...142 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:139: Met Lys Thr Asn Phe Tyr Lys Ile Lys Leu Leu Phe Ala Trp Cys Leu 1 5 10 15 Ile Ile Gly Met Phe Asn Ala Pro Leu Asn Ala Asp Gln Asn Thr Asp 20 25 30 Ile Lys Asp Ile Ser Pro Glu Asp Met Ala Leu Asn Ser Val Gly Leu 35 40 45 Val Ser Arg Asp Gln Leu Lys Ile Glu Ile Pro Lys Glu Thr Leu Glu 50 55 60 Gln Lys Val Ala Ile Leu Asn Asp Tyr Asn Asp Lys Asn Val Asn Ile 65 70 75 80 Lys Phe Asp Asp Ile Ser Leu Gly Ser Phe Gln Pro Asn Asp Asn Leu 85 90 95 Gly Ile Asn Ala Met Trp Gly Ile Gln Asn Leu Leu Met Ser Gln Met 100 105 110 Met Ser Asn Tyr Gly Pro Asn Asn Ser Phe Met Tyr Gly Tyr Ala Pro 115 120 125 Thr Tyr Ser Asp Ser Ser Phe Leu Pro Pro Ile Leu Gly Tyr 130 135 140 (2) INFORMATION FOR SEQ ID NO:140: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 208 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES

(vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...208 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:140: Leu Ile Asn Asn Asn Asn Asn Asn Lys Lys Leu Arg Gly Phe Phe Leu 1 5 10 15 Lys Val Leu Leu Ser Leu Val Val Phe Ser Ser Tyr Gly Ser Ala Asn 20 25 30 Asp Asp Lys Glu Ala Lys Lys Glu Ala Leu Glu Lys Glu Lys Asn Thr 35 40 45 Pro Asn Gly Leu Val Tyr Thr Asn Leu Asp Phe Asp Ser Phe Lys Ala 50 55 60 Thr Ile Lys Asn Leu Lys Asp Lys Lys Val Thr Phe Lys Glu Val Asn 65 70 75 80 Pro Asp Ile Ile Lys Asp Glu Val Phe Asp Phe Val Ile Val Asn Arg 85 90 95 Val Leu Lys Lys Ile Lys Asp Leu Lys His Tyr Asp Pro Val Ile Glu 100 105 110 Lys Ile Phe Asp Glu Lys Gly Lys Glu Met Gly Leu Asn Val Glu Leu 115 120 125 Gln Ile Asn Pro Glu Val Lys Asp Phe Phe Thr Phe Lys Ser Ile Ser 130 135 140 Thr Thr Asn Lys Gln Arg Cys Phe Leu Ser Leu His Gly Glu Thr Arg 145 150 155 160 Glu Ile Leu Cys Asp Asp Lys Leu Tyr Asn Val Leu Leu Ala Val Phe 165 170 175 Asn Ser Tyr Asp Pro Asn Asp Leu Leu Lys His Ile Ser Thr Ile Glu 180 185 190 Ser Leu Lys Lys Ile Phe Tyr Thr Ile Thr Cys Glu Ala Val Tyr Leu 195 200 205 (2) INFORMATION FOR SEQ ID NO:141: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 245 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...245 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:141:

Met Ala Gly Thr Gln Ala Ile Tyr Glu Ser Ser Ser Ala Gly Phe Leu 1 5 10 15 Ser Gln Val Ser Ser Ile Ile Ser Ser Thr Ser Gly Val Ala Gly Pro 20 25 30 Phe Ala Gly Ile Val Ala Gly Ala Met Thr Ala Ala Ile Ile Pro Ile 35 40 45 Val Val Gly Phe Thr Asn Pro Gln Met Thr Ala Ile Met Thr Gln Tyr 50 55 60 Asn Gln Ser Ile Ala Glu Ala Val Ser Val Pro Met Lys Ala Ala Asn 65 70 75 80 Gln Gln Tyr Asn Gln Leu Tyr Gln Gly Phe Asn Asp Gln Ser Met Ala 85 90 95 Val Gly Asn Asn Ile Leu Asn Ile Ser Lys Leu Thr Gly Glu Phe Asn 100 105 110 Ala Gln Gly Asn Thr Gln Ser Ala Gln Ile Ser Ala Val Asn Ser Gln 115 120 125 Ile Ala Ser Ile Leu Ala Ser Asn Thr Thr Pro Lys Asn Pro Ser Ala 130 135 140 Ile Glu Ala Tyr Ala Thr Asn Gln Ile Ala Val Pro Ser Val Pro Thr 145 150 155 160 Thr Val Glu Met Met Ser Gly Ile Leu Gly Asn Ile Thr Ser Ala Ala 165 170 175 Pro Lys Tyr Ala Leu Ala Leu Gln Glu Gln Leu Arg Ser Gln Ala Ser 180 185 190 Asn Ser Ser Met Asn Asp Thr Ala Asp Ser Leu Asp Ser Cys Thr Ala 195 200 205 Leu Gly Ala Leu Val Gly Ser Ser Lys Val Phe Phe Ser Cys Met Gln 210 215 220 Ile Ser Met Thr Pro Met Ser Val Ser Met Pro Thr Val Met Pro Asn 225 230 235 240 Thr Ser Gly Cys His 245 (2) INFORMATION FOR SEQ ID NO:142: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 367 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...367 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:142: Met Ile Lys Ser Val Glu Ile Glu Asn Tyr Lys Asn Phe Glu His Leu

1 5 10 15 Lys Met Glu Asn Phe Lys Leu Ile Asn Phe Phe Thr Gly Gln Asn Asp 20 25 30 Ala Gly Lys Thr Asn Leu Leu Glu Ala Leu Tyr Thr Asn Thr Gly Leu 35 40 45 Cys Asp Pro Thr Ala Asn Gln Val Ser Leu Pro Pro Glu His Ala Val 50 55 60 Asn Ile Ser Glu Phe Arg Lys Ile Lys Leu Asp Ala Asp Asn Leu Lys 65 70 75 80 Thr Phe Phe Tyr Gln Gly Asn Thr Ala Asn Pro Ile Ser Ile Arg Thr 85 90 95 Glu Phe Glu His Ala Thr Ile Pro Leu Thr Ile Gln Tyr Pro Thr Gln 100 105 110 Thr Ser Tyr Ser Lys Asp Ile Asn Leu Asn Ser Asp Asp Ala His Met 115 120 125 Thr Asn Leu Ile Asn Thr Thr Ile Thr Lys Pro Gln Leu Gln Phe Ser 130 135 140 Tyr Asn Pro Ser Leu Ser Pro Met Thr Met Thr Tyr Glu Phe Glu Arg 145 150 155 160 Gln Asn Leu Gly Leu Ile His Ser Asn Leu Asp Lys Ile Ala Gln Thr 165 170 175 Tyr Lys Glu Asn Ala Met Phe Ile Pro Ile Glu Leu Ser Ile Val Asn 180 185 190 Ser Leu Lys Ala Leu Glu Asn Leu Gln Leu Ala Ser Lys Glu Lys Glu 195 200 205 Leu Ile Glu Ile Leu Gln Cys Phe Asn Pro Asn Ile Leu Asn Ala Asn 210 215 220 Thr Ile Arg Lys Ser Val Tyr Ile Gln Ile Lys Asp Glu Asn Thr Pro 225 230 235 240 Leu Glu Glu Ser Pro Lys Arg Leu Leu Asn Leu Phe Gly Trp Gly Phe 245 250 255 Ile Lys Phe Phe Ile Met Val Ser Ile Leu Ile Asp Asn Arg Val Lys 260 265 270 Tyr Leu Phe Ile Asp Glu Ile Glu Ser Gly Leu His His Thr Lys Met 275 280 285 Gln Glu Phe Leu Lys Ala Leu Phe Lys Leu Ala Gln Lys Leu Gln Ile 290 295 300 Gln Ile Phe Ala Thr Thr His Asn Lys Glu Phe Leu Leu Asn Ala Ile 305 310 315 320 Asn Thr Ile Ser Asp Asn Glu Thr Gly Val Phe Lys Asp Ile Ala Leu 325 330 335 Phe Glu Leu Glu Lys Glu Ser Ala Ser Gly Phe Ile Arg His Ser Tyr 340 345 350 Ser Met Leu Glu Lys Ala Leu Tyr Arg Gly Met Glu Val Arg Gly 355 360 365 (2) INFORMATION FOR SEQ ID NO:143: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 409 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein

(iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...409 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:143: Met Ser Leu Ile Arg Val Asn Gly Glu Ala Phe Lys Leu Ser Leu Glu 1 5 10 15 Ser Leu Glu Glu Asp Pro Phe Glu Thr Lys Glu Thr Leu Glu Thr Leu 20 25 30 Glu Thr Leu Ile Lys Gln Thr Ser Val Val Leu Leu Ala Ala Gly Glu 35 40 45 Ser Lys Arg Phe Ser Arg Ala Ile Lys Lys Gln Trp Leu Arg Ser His 50 55 60 His Thr Pro Leu Trp Leu Ser Val Tyr Glu Ser Phe Lys Glu Ala Leu 65 70 75 80 Asp Phe Lys Glu Val Ile Leu Val Val Ser Glu Leu Asp Tyr Val Tyr 85 90 95 Ile Gln Arg His Tyr Pro Lys Ile Lys Leu Val Lys Gly Gly Ala Ser 100 105 110 Arg Gln Glu Ser Val Arg Asn Ala Leu Lys Val Ile Asp Ser Thr Tyr 115 120 125 Thr Ile Thr Ser Asp Val Ala Arg Gly Leu Ala Asn Met Glu Ala Leu 130 135 140 Lys Ser Leu Phe Leu Thr Leu Gln Gln Thr Ser His Tyr Cys Ile Ala 145 150 155 160 Pro Tyr Leu Pro Cys Tyr Asp Thr Ala Ile Tyr Tyr Asn Glu Ala Leu 165 170 175 Asp Arg Glu Ala Ile Lys Leu Ile Gln Thr Pro Gln Leu Ser His Thr 180 185 190 Lys Thr Leu Gln Ser Ala Leu Asn Gln Gly Gly Phe Lys Asp Glu Ser 195 200 205 Ser Ala Ile Leu Gln Ala Phe Pro Asn Ser Val Ser Tyr Ile Glu Gly 210 215 220 Ser Lys Asp Leu His Lys Leu Thr Thr Ser Gly Asp Leu Lys Phe Phe 225 230 235 240 Thr Pro Phe Phe Asn Pro Ala Lys Asp Thr Phe Ile Gly Met Gly Phe 245 250 255 Asp Thr His Ala Phe Ile Lys Asp Lys Pro Met Val Leu Gly Gly Val 260 265 270 Val Leu Asp Cys Glu Phe Gly Leu Lys Ala His Ser Asp Gly Asp Ala 275 280 285 Leu Leu His Ala Val Ile Asp Ala Ile Leu Gly Ala Ile Lys Gly Gly 290 295 300 Asp Ile Gly Glu Trp Phe Pro Asp Asn Asp Pro Lys Tyr Lys Asn Ala 305 310 315 320 Ser Ser Lys Glu Leu Leu Lys Ile Val Leu Asp Phe Ser Gln Ser Ile 325 330 335 Gly Phe Glu Leu Leu Glu Met Gly Ala Thr Ile Phe Ser Glu Ile Pro 340 345 350

Lys Ile Thr Pro Tyr Lys Pro Ala Ile Leu Glu Asn Leu Ser Gln Leu 355 360 365 Leu Gly Leu Glu Lys Ser Gln Ile Ser Leu Lys Ala Thr Thr Met Glu 370 375 380 Lys Met Gly Phe Ile Gly Lys Gln Glu Gly Leu Leu Val Gln Ala His 385 390 395 400 Val Ser Met Arg Tyr Lys Gln Lys Leu 405 (2) INFORMATION FOR SEQ ID NO:144: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 270 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...270 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:144: Met Lys Lys Phe Val Ala Leu Gly Leu Leu Ser Ala Val Leu Ser Ser 1 5 10 15 Ser Leu Leu Ala Glu Gly Asp Gly Val Tyr Ile Gly Thr Asn Tyr Gln 20 25 30 Leu Gly Gln Ala Arg Leu Asn Ser Asn Ile Tyr Asn Thr Gly Asp Cys 35 40 45 Thr Gly Ser Val Val Gly Cys Pro Pro Gly Leu Thr Ala Asn Lys His 50 55 60 Asn Pro Gly Gly Thr Asn Ile Asn Trp His Ser Lys Tyr Ala Asn Gly 65 70 75 80 Ala Leu Asn Gly Phe Gly Leu Asn Val Gly Tyr Lys Lys Phe Phe Gln 85 90 95 Phe Lys Ser Leu Asp Met Thr Ser Lys Trp Phe Gly Phe Arg Val Tyr 100 105 110 Gly Leu Phe Asp Tyr Gly His Ala Asp Leu Gly Lys Gln Val Tyr Ala 115 120 125 Pro Asn Lys Ile Gln Leu Asp Met Val Ser Trp Gly Val Gly Ser Asp 130 135 140 Leu Leu Ala Asp Ile Ile Asp Lys Asp Asn Ala Ser Phe Gly Ile Phe 145 150 155 160 Gly Gly Val Ala Ile Gly Gly Asn Thr Trp Lys Ser Ser Ala Ala Asn 165 170 175 Tyr Trp Lys Glu Gln Ile Ile Glu Ala Lys Gly Pro Asp Val Cys Thr 180 185 190 Pro Thr Tyr Cys Asn Pro Asn Ala Pro Tyr Ser Thr Asn Thr Ser Thr 195 200 205

Val Ala Phe Gln Val Trp Leu Asn Phe Gly Val Arg Ala Asn Ile Tyr 210 215 220 Lys His Asn Gly Val Glu Phe Gly Val Arg Val Pro Leu Leu Ile Asn 225 230 235 240 Lys Phe Leu Ser Ala Gly Pro Asn Ala Thr Asn Leu Tyr Tyr His Leu 245 250 255 Lys Arg Asp Tyr Ser Leu Tyr Leu Gly Tyr Asn Tyr Thr Phe 260 265 270 (2) INFORMATION FOR SEQ ID NO:145: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 438 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...438 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:145: Met Ala Tyr Lys Pro Asn Lys Lys Lys Leu Lys Glu Leu Arg Glu Gln 1 5 10 15 Pro Asn Leu Phe Ser Ile Leu Asp Lys Gly Asp Val Ala Thr Asn Asn 20 25 30 Pro Val Glu Glu Ser Asp Lys Ala Asn Lys Ile Gln Glu Pro Leu Pro 35 40 45 Tyr Val Val Lys Thr Gln Ile Asn Lys Ala Ser Met Ile Ser Arg Asp 50 55 60 Pro Ile Glu Trp Ala Lys Tyr Leu Ser Phe Glu Lys Arg Val Tyr Lys 65 70 75 80 Asp Asn Ser Lys Glu Asp Val Asn Phe Phe Ala Asn Gly Glu Ile Lys 85 90 95 Glu Ser Ser Arg Val Tyr Glu Ala Asn Lys Glu Gly Phe Glu Arg Arg 100 105 110 Ile Thr Lys Arg Tyr Asp Leu Ile Asp Arg Asn Ile Asp Arg Asn Arg 115 120 125 Glu Phe Phe Ile Lys Glu Ile Glu Ile Leu Thr His Thr Asn Ser Leu 130 135 140 Lys Glu Leu Lys Glu Gln Gly Leu Glu Ile Gln Leu Thr His His Asn 145 150 155 160 Glu Thr His Lys Lys Ala Leu Glu Asn Gly Asn Glu Ile Val Lys Glu 165 170 175 Tyr Asp His Leu Lys Asp Ile Tyr Gln Glu Val Glu Arg Thr Lys Asp 180 185 190 Gly Gly Leu Val Arg Glu Ile Ile Pro Ser Ile Ser Ser Ala Glu Tyr 195 200 205

Phe Lys Leu Tyr Asn Lys Leu Pro Phe Glu Ser Ile Asn Asn Glu Asn 210 215 220 Thr Lys Leu Asn Thr Asn Asp Asn Glu Glu Val Lys Lys Leu Glu Phe 225 230 235 240 Glu Leu Ala Lys Glu Val His Ile Leu Ile Leu Glu Gln Gln Leu Leu 245 250 255 Ser Ala Thr Asn Tyr Tyr Ser Trp Ile Asp Lys Asp Asp Asn Ala Asn 260 265 270 Phe Ala Trp Lys Met His Arg Leu Ile Asn Glu Asn Lys Leu Lys Glu 275 280 285 Asn His Leu Ser Ala Asn Asn Ala Asn Lys Ile Lys Gln Phe Phe Phe 290 295 300 Asn Asn Gly Ser Ile Leu Gly Trp Thr Lys Glu Glu Gln Ser Ala Ile 305 310 315 320 Gln Glu Asn Arg Asp Tyr Ser Leu Arg Ser Ala Leu Leu Ser Leu Glu 325 330 335 Glu Ile Ala Gln Ala Lys Ile Glu Leu Gln Lys Tyr Tyr Glu Ser Val 340 345 350 Tyr Val Asn Gly Asp Gly Asn Lys Arg Glu Ile Lys Pro Phe Lys Glu 355 360 365 Ile Leu Arg Asp Thr Asn Asn Phe Glu Lys Ala Tyr Lys Glu Arg Tyr 370 375 380 Asp Lys Leu Val Ser Leu Ser Ala Ala Ile Ile Gln Ala Lys Glu Gly 385 390 395 400 Gly Asn Glu Arg Pro Asn Ser Ser Ala Asn Asn Asn Asn Pro Ile Lys 405 410 415 Asn Thr Ile Glu Thr Asn Thr Ser Asn Asn Ile Ile Gln Asn Asn Asp 420 425 430 Asn Ile Ile Ile Gln Ile 435 (2) INFORMATION FOR SEQ ID NO:146: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 215 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...215 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:146: Met Gln Ala Leu Lys Ser Leu Leu Glu Val Ile Thr Lys Leu Gln Asn 1 5 10 15 Leu Gly Gly Tyr Leu Met His Ile Ala Ile Phe Ile Ile Phe Ile Trp 20 25 30

Ile Gly Gly Leu Lys Phe Val Pro Tyr Glu Ala Glu Gly Ile Ala Pro 35 40 45 Phe Val Ala Asn Ser Pro Phe Phe Ser Phe Met Tyr Lys Phe Glu Lys 50 55 60 Pro Ala Tyr Lys Gln His Lys Met Ser Glu Ser Gln Ser Met Gln Glu 65 70 75 80 Glu Met Gln Asp Asn Pro Lys Ile Val Glu Asn Lys Glu Trp His Lys 85 90 95 Glu Asn Arg Thr Tyr Leu Val Ala Glu Gly Leu Gly Ile Thr Ile Met 100 105 110 Ile Leu Gly Ile Leu Val Leu Leu Gly Leu Trp Met Pro Leu Met Gly 115 120 125 Val Val Gly Gly Leu Leu Val Ala Gly Met Thr Ile Thr Thr Leu Ser 130 135 140 Phe Leu Phe Thr Thr Pro Glu Val Phe Val Asn Gln His Phe Pro Trp 145 150 155 160 Leu Ser Gly Ala Gly Arg Leu Val Val Lys Asp Leu Ala Leu Phe Ala 165 170 175 Gly Gly Leu Phe Val Ala Gly Phe Asp Ala Lys Arg Tyr Leu Glu Gly 180 185 190 Lys Gly Phe Cys Leu Met Asp Arg Ser Ser Val Gly Ile Lys Thr Lys 195 200 205 Cys Ser Ser Gly Cys Cys Ser 210 215 (2) INFORMATION FOR SEQ ID NO:147: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1,..20 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:147: TATACCATGG TGGGCGCTAA 20 (2) INFORMATION FOR SEQ ID NO:148: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 23 base pairs (B) TYPE: nucleic acid

(C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...23 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:148: ATGAATTCGA GTAAGGATTT TTG 23 (2) INFORMATION FOR SEQ ID NO:149: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 22 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...22 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:149: TTAACCATGG TGAAAAGCGA TA 22 (2) INFORMATION FOR SEQ ID NO:150: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic)

(iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...23 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:150: TAGAATTCGC ATAACGATCA ATC 23 (2) INFORMATION FOR SEQ ID NO:151: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 22 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...22 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:151: ATATCCATGG TGAGTTTGAT GA 22 (2) INFORMATION FOR SEQ ID NO:152: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 25 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE:

(A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...25 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:152: ATGAATTCAA TTTTTTATTT TGCCA 25 (2) INFORMATION FOR SEQ ID NO:153: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...21 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:153: AATTCCATGG TGGGGGCTAT G 21 (2) INFORMATION FOR SEQ ID NO:154: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...23

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:154: ATGAATTCTC GATAGCCAAA ATC 23 (2) INFORMATION FOR SEQ ID NO:155: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 25 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...25 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:155: AATTCCATGG TGCATAACTT CCATT 25 (2) INFORMATION FOR SEQ ID NO:156: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 25 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...25 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:156: AAGAATTCTC TAGCATCCAA ATGGA 25

(2) INFORMATION FOR SEQ ID NO:157: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 24 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...24 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:157: ATTTCCATGG TCATGTCTCA TATT 24 (2) INFORMATION FOR SEQ ID NO:158: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...23 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:158: ATGAATTCCA TCTTTTATTC CAC 23 (2) INFORMATION FOR SEQ ID NO:159: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 27 base pairs (B) TYPE: nucleic acid

(C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...27 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:159: AACCATGGTG ATTTTAAGCA TTGAAAG 27 (2) INFORMATION FOR SEQ ID NO:160: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 28 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...28 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:160: AAGAATTCCA CTCAAAATTT TTTAACAG 28 (2) INFORMATION FOR SEQ ID NO:161: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 25 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic)

(iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...25 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:161: GATCATCCAT ATGTTATCTT CTAAT 25 (2) INFORMATION FOR SEQ ID NO:162: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...23 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:162: TGAATTCAAC CATTTTAACC CTG 23 (2) INFORMATION FOR SEQ ID NO:163: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 27 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE:

(A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...27 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:163: TATACCATGG TGAAATTTTT TCTTTTA 27 (2) INFORMATION FOR SEQ ID NO:164: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 25 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...25 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:164: AGAATTCAAT TGCGTCTTGT AAAAG 25 (2) INFORMATION FOR SEQ ID NO:165: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 24 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...24

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:165: TATACCATGG TGATGGACAA ACTC 24 (2) INFORMATION FOR SEQ ID NO:166: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...23 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:166: ATGAATTCCC ACTTGGGGCG ATA 23 (2) INFORMATION FOR SEQ ID NO:167: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 25 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...25 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:167: TTATGGATCC AAACCAATTA AAACT 25

(2) INFORMATION FOR SEQ ID NO:168: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1. ..23 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:168: TATCTCGAGT TATAGAGAAG GGC 23 (2) INFORMATION FOR SEQ ID NO:169: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 22 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1.. .22 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:169: TTAACCATGG TGAAAAGCGA TA 22 (2) INFORMATION FOR SEQ ID NO:170: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 24 base pairs (B) TYPE: nucleic acid

(C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...24 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:170: TAGAATTCGC CTCTAAAACT TTAG 24 (2) INFORMATION FOR SEQ ID NO:171: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 22 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...22 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:171: TTAACCATGG TGAAAAGCGA TA 22 (2) INFORMATION FOR SEQ ID NO:172: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic)

(iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...23 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:172: TAGAATTCGC ATAACGATCA ATC 23 (2) INFORMATION FOR SEQ ID NO:173: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 22 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...22 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:173: ATATCCATGG TGAGTTTGAT GA 22 (2) INFORMATION FOR SEQ ID NO:174: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 25 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE:

(A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...25 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:174: ATGAATTCAA TTTTTTATTT TGCCA 25 (2) INFORMATION FOR SEQ ID NO:175: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...23 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:175: AATTCCATGG CTATCCAAAT CCG 23 (2) INFORMATION FOR SEQ ID NO:176: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 25 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...25

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:176: ATGAATTCGC CAAAATCGTA GTATT 25 (2) INFORMATION FOR SEQ ID NO:177: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 24 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...24 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:177: GATACCATGG AATTTATGAA AAAG 24 (2) INFORMATION FOR SEQ ID NO:178: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 25 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...25 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:178: TGAATTCGAA AAAGTGTAGT TATAC 25

(2) INFORMATION FOR SEQ ID NO:179: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...19 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:179: CCCTTCATTT TAGAAATCG 19 (2) INFORMATION FOR SEQ ID NO:180: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...20 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:180: ATTTCAACCA ATTCAATGCG 20 (2) INFORMATION FOR SEQ ID NO:181: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE: nucleic acid

(C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...20 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:181: GCCCCTTTTG ATTTGAAGCT 20 (2) INFORMATION FOR SEQ ID NO:182: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 22 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...22 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:182: TCGCTCCAAG ATACCAAGAA GT 22 (2) INFORMATION FOR SEQ ID NO:183: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 22 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic)

(iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...22 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:183: CTTGAATTAG GGGCAAAGAT CG 22 (2) INFORMATION FOR SEQ ID NO:184: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 22 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...22 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:184: ATGCGTTTTT ACCCAAAGAA GT 22 (2) INFORMATION FOR SEQ ID NO:185: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 22 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE:

(A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...22 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:185: ATAACGCCAC TTCCTTATTG GT 22 (2) INFORMATION FOR SEQ ID NO:186: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...19 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:186: CTTTGGGTAA AAACGCATC 19 (2) INFORMATION FOR SEQ ID NO:187: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...20

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:187: CGATCTTTGA TCCTAATTCA 20 (2) INFORMATION FOR SEQ ID NO:188: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...19 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:188: ATCAAGTTGC CTATGCTGA 19 (2) INFORMATION FOR SEQ ID NO:189: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 22 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...22 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:189: TTGAACACTT TTGATTATGC GG 22

(2) INFORMATION FOR SEQ ID NO:190: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...23 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:190: GGATTATGCG ATTGTTTTAC AAG 23 (2) INFORMATION FOR SEQ ID NO:191: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...21 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:191: GTCTTTAGCA AAAATGGCGT C 21 (2) INFORMATION FOR SEQ ID NO:192: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 base pairs (B) TYPE: nucleic acid

(C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...21 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:192: AATGAGCGTA AGAGAGCCTT C 21 (2) INFORMATION FOR SEQ ID NO:193: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...18 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:193: CTTATGGGGG TATTGTCA 18 (2) INFORMATION FOR SEQ ID NO:194: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic)

(iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...18 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:194: AGCATGTGGG TATCCAGC 18 (2) INFORMATION FOR SEQ ID NO:195: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...19 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:195: AGGTTGTTGC CTAAAGACT 19 (2) INFORMATION FOR SEQ ID NO:196: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE:

(A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...18 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:196: CTGCCTCCAC CTTTGATC 18 (2) INFORMATION FOR SEQ ID NO:197: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...19 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:197: ACCAATATCA ATTGGCACT 19 (2) INFORMATION FOR SEQ ID NO:198: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...18

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:198: ACTTGGAAAA GCTCTGCA 18 (2) INFORMATION FOR SEQ ID NO:199: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...19 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:199: CTTGCTTGTC ATATCTAGC 19 (2) INFORMATION FOR SEQ ID NO:200: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...18 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:200: GTTGAAGTGT TGGTGCTA 18

(2) INFORMATION FOR SEQ ID NO:201: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 22 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...22 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:201: CAAGCAAGTG GTTTGGTTTT AG 22 (2) INFORMATION FOR SEQ ID NO:202: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 22 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...22 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:202: TGGAAAGAGC AAATCATTGA AG 22 (2) INFORMATION FOR SEQ ID NO:203: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 base pairs (B) TYPE: nucleic acid

(C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...21 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:203: GCCCATAATC AAAAAGCCCA T 21 (2) INFORMATION FOR SEQ ID NO:204: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 24 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...24 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:204: CTAAAACCAA ACCACTTGCT TGTC 24 (2) INFORMATION FOR SEQ ID NO:205: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 16 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic)

(iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...16 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:205: GTAAAACGAC GGCCAG 16 (2) INFORMATION FOR SEQ ID NO:206: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 17 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION 1...17 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:206: CAGGAAACAG CTATGAC 17 (2) INFORMATION FOR SEQ ID NO:207: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 base pairs (B) TYPE: nucl-eic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE:

(A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...21 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:207: ATCTTACCTA TCACCTCAAA T 21 (2) INFORMATION FOR SEQ ID NO:208: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Helicobacter pylori (ix) FEATURE: (A) NAME/KEY: misc~feature (B) LOCATION 1...21 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:208: AGACAGCAAC ATCTTTGTGA A 21