NUCLEIC ACID AMPLIFICATION PRIMER FOR DETECTING RBCS-1A GENE AND METHOD OF QUANTIFYING NUCLEIC ACID BY USING THE GENE AS INTERNAL STANDARD

WIPO Patent Application WO/2006/035712

A1

A primer for amplifying a nucleic acid characterized by being hybridizable with a target nucleic acid sequence containing at least one member selected from the group consisting of ribulose bisphosphate carboxylase small chain 1A: RBCS-1A) gene, mRNA of this gene, cDNA of this gene and partial sequences thereof and having a first sequence hybridizable with a part of the target nucleic acid sequence as described above in the 3’-terminal side and a second sequence hybridizable with a part of a complementary chain of the target nucleic acid sequence as described above in the 5’-terminal side; and a method of quantifying a nucleic acid by using at least one member selected from among the above-described gene, mRNA of the gene, cDNA of the gene and partial sequences thereof as an internal standard.

DAITO MOTONARI (JP)
YAMAGATA KOICHI (JP)

PCT/JP2005/017628

April 06, 2006

September 26, 2005

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SYSMEX CORP (JP)
DAITO MOTONARI (JP)
YAMAGATA KOICHI (JP)

C12Q1/68; C12N15/09

WO2000028082A1	2000-05-18
WO2002024902A1	2002-03-28

JP2004187604A	2004-07-08
JPH10304890A	1998-11-17

KREBBERS E ET AL: "Four genes in two diverged subfamilies encode the ribulose-l,5- bisphosphate carboxylase small subunit polypeptides of Arabidopsis thaliana.", PLAN MOL BIOL., vol. 11, 1988, pages 745 - 759, XP002967146

Nogawa, Shintaro (Minamimorimachi Park Bldg. 1-3, Nishitenma 5-chome, Kita-ku, Osaka-sh, Osaka 47, JP)

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