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Title:
NUCLEIC ACID ANALYSIS
Document Type and Number:
WIPO Patent Application WO2006046144
Kind Code:
A3
Abstract:
The invention relates to a method for analysing nucleic acid isolated from or in a biological sample, comprising the steps of hybridising to the nucleic acid(s) under desired conditions in solution a repertoire of identification oligonucleotides each of defined and different mass; capturing the nucleic acid onto a solid phase and washing off those members of the repertoire which are not hybridised to the nucleic acid with a desired affinity; and eluting the repertoire members which remain hybridised to nucleic acid after the washing step and analysing said members to resolve their mass and/or quantity.

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Inventors:
GROSVELD FRANKLIN (NL)
PHILIPSEN JACOBUS (NL)
Application Number:
PCT/IB2005/003513
Publication Date:
November 23, 2006
Filing Date:
October 26, 2005
Export Citation:
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Assignee:
UNIV ERASMUS MEDICAL CT (NL)
GROSVELD FRANKLIN (NL)
PHILIPSEN JACOBUS (NL)
International Classes:
C12Q1/68
Domestic Patent References:
WO2002086051A22002-10-31
WO2004022779A22004-03-18
Foreign References:
US20030228594A12003-12-11
Other References:
ROSS ET AL: "DISCRIMINATION OF SINGLE-NUCLEOTIDE POLYMORPHISMS IN HUMAN DNA USING PEPTIDE NUCLEIC ACID PROBES DETECTED BY MALDI-TOF MASS SPECTROMETRY", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY. COLUMBUS, US, vol. 69, no. 20, 15 October 1997 (1997-10-15), pages 4197 - 4202, XP002113518, ISSN: 0003-2700
GRIFFIN ET AL: "GENETIC ANALYSIS BY PEPTIDE NUCLEIC ACID AFFINITY MALDI-TOF MASS SPECTROMETRY", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP, NEW YORK, NY, US, vol. 15, December 1997 (1997-12-01), pages 1368 - 1372, XP002113517, ISSN: 1087-0156
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