Title:
NUCLEIC ACID CAPABLE OF BINDING SPECIFICALLY TO Ras TARGET PROTEIN
Document Type and Number:
WIPO Patent Application WO/2000/009684
Kind Code:
A1
Abstract:
A novel nucleic acid (aptamer) which binds specifically to the target protein of Ras, more particularly, a novel RNA aptamer which binds specifically to Raf-1; a method for screening an RNA capable of binding specifically to the target protein of Ras which comprises selecting an RNA capable of binding to the target protein of Ras from a pool of RNAs having various base sequences; a method for regulating the transmission of a signal causing the proliferation or differentiation of cells by using the above-described nucleic acid; and medicinal compositions with the use of the same.
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Inventors:
YOKOYAMA SHIGEYUKI (JP)
HIRAO ICHIRO (JP)
SAKAMOTO KENSAKU (JP)
HIRAO ICHIRO (JP)
SAKAMOTO KENSAKU (JP)
Application Number:
PCT/JP1999/004399
Publication Date:
February 24, 2000
Filing Date:
August 13, 1999
Export Citation:
Assignee:
JAPAN SCIENCE & TECH CORP (JP)
YOKOYAMA SHIGEYUKI (JP)
HIRAO ICHIRO (JP)
SAKAMOTO KENSAKU (JP)
YOKOYAMA SHIGEYUKI (JP)
HIRAO ICHIRO (JP)
SAKAMOTO KENSAKU (JP)
International Classes:
C12N15/113; C12Q1/68; A61K38/00; (IPC1-7): C12N15/11; A61K48/00; A61K31/70; C12Q1/68
Other References:
MICHIKO KIMOTO ET AL.: "RNA aptamers that specifically bind to the Ras-binding domain of Raf-1", FEBS LETTERS,, vol. 441, no. 2, December 1998 (1998-12-01), pages 322 - 326, XP002926506
WALTER KOLCH ET AL.: "Inhibition of Raf-1 signaling by a monoclonal antibody, which interferes with Raf-1 activation and with Mek substrate binding", ONCOGENE,, vol. 13, no. 6, 1996, pages 1305 - 1314, XP002926507
ANDREW D. ELLINGTON ET AL.: "In vitro selection of RNA molecules that bind specific ligands", NATURE,, vol. 346, no. 6287, 1990, pages 818 - 822, XP002926508
CRAIG TUERK ET AL.: "Systematic evolution of ligands by exponential enrichment:RNA ligands to bacteriophage T4 DNA polymerase", SCIENCE,, vol. 249, no. 4968, 1990, pages 505 - 510, XP002926509
See also references of EP 1026243A4
WALTER KOLCH ET AL.: "Inhibition of Raf-1 signaling by a monoclonal antibody, which interferes with Raf-1 activation and with Mek substrate binding", ONCOGENE,, vol. 13, no. 6, 1996, pages 1305 - 1314, XP002926507
ANDREW D. ELLINGTON ET AL.: "In vitro selection of RNA molecules that bind specific ligands", NATURE,, vol. 346, no. 6287, 1990, pages 818 - 822, XP002926508
CRAIG TUERK ET AL.: "Systematic evolution of ligands by exponential enrichment:RNA ligands to bacteriophage T4 DNA polymerase", SCIENCE,, vol. 249, no. 4968, 1990, pages 505 - 510, XP002926509
See also references of EP 1026243A4
Attorney, Agent or Firm:
Saeki, Norio (9th floor 15-2, Nihonbashi 3-chome Chuo-ku Tokyo, JP)
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