Title:
NUCLEIC ACID FOR DEMETHYLATION OF OASIS GENE AND DEMETHYLATION METHOD USING SAME
Document Type and Number:
WIPO Patent Application WO/2021/246165
Kind Code:
A1
Abstract:
A nucleic acid according to the present invention for the demethylation of the OASIS gene comprises, located under the control of a promoter sequence, a base sequence that encodes a dCas9/demethylation enzyme fusion protein and a base sequence that encodes a guide RNA. Designating the +1 position as the first base at the translation starting point of the OASIS gene, the guide RNA has a base sequence that recognizes all or part of the base sequence from position -327 to position +511.
Inventors:
IMAIZUMI KAZUNORI (JP)
SAITO ATSUSHI (JP)
SAITO ATSUSHI (JP)
Application Number:
PCT/JP2021/018886
Publication Date:
December 09, 2021
Filing Date:
May 19, 2021
Export Citation:
Assignee:
UNIV HIROSHIMA (JP)
International Classes:
C12N15/09; A61K31/711; A61K38/43; A61K38/46; A61K48/00; A61P35/00; A61P43/00; C12N15/62
Domestic Patent References:
| WO2017090724A1 | 2017-06-01 |
Foreign References:
| JP2016512264A | 2016-04-25 |
Other References:
ROSE MICHAEL, SCHUBERT CLAUDIA, DIERICHS LAURA, GAISA NADINE T, HEER MATTHIAS, HEIDENREICH AXEL, KNÜCHEL RUTH, DAHL EDGAR: "OASIS/CREB3L1 is epigenetically silenced in human bladder cancer facilitating tumor cell spreading and migration in vitro", EPIGENETICS, LANDES BIOSCIENCE, US, vol. 9, no. 12, 2 December 2014 (2014-12-02), US , pages 1626 - 1640, XP055880583, ISSN: 1559-2294, DOI: 10.4161/15592294.2014.988052
SUMIYO MORITA, HIROFUMI NOGUCHI, TAKURO HORII, KAZUHIKO NAKABAYASHI, MIKA KIMURA, KOHJI OKAMURA, ATSUHIKO SAKAI, HIDEYUKI NAKASHIM: "Targeted DNA demethylation in vivo using dCas9–peptide repeat and scFv–TET1 catalytic domain fusions", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP US, NEW YORK, vol. 34, no. 10, 29 October 2016 (2016-10-29), New York, pages 1060 - 1065, XP055459997, ISSN: 1087-0156, DOI: 10.1038/nbt.3658
WARD ALISON K., MELLOR PAUL, SMITH SHARI E., KENDALL STEPHANIE, JUST NATASHA A., VIZEACOUMAR FREDERICK S., SARKER SABUJ, PHILLIPS : "Epigenetic silencing of CREB3L1 by DNA methylation is associated with high-grade metastatic breast cancers with poor prognosis and is prevalent in triple negative breast cancers", BREAST CANCER RESEARCH, vol. 18, no. 1, 1 December 2016 (2016-12-01), XP055880585, DOI: 10.1186/s13058-016-0672-x
SUMIYO MORITA, HIROFUMI NOGUCHI, TAKURO HORII, KAZUHIKO NAKABAYASHI, MIKA KIMURA, KOHJI OKAMURA, ATSUHIKO SAKAI, HIDEYUKI NAKASHIM: "Targeted DNA demethylation in vivo using dCas9–peptide repeat and scFv–TET1 catalytic domain fusions", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP US, NEW YORK, vol. 34, no. 10, 29 October 2016 (2016-10-29), New York, pages 1060 - 1065, XP055459997, ISSN: 1087-0156, DOI: 10.1038/nbt.3658
WARD ALISON K., MELLOR PAUL, SMITH SHARI E., KENDALL STEPHANIE, JUST NATASHA A., VIZEACOUMAR FREDERICK S., SARKER SABUJ, PHILLIPS : "Epigenetic silencing of CREB3L1 by DNA methylation is associated with high-grade metastatic breast cancers with poor prognosis and is prevalent in triple negative breast cancers", BREAST CANCER RESEARCH, vol. 18, no. 1, 1 December 2016 (2016-12-01), XP055880585, DOI: 10.1186/s13058-016-0672-x
Attorney, Agent or Firm:
ISEKI Katsumori et al. (JP)
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