Title:
NUCLEIC ACID ENCAPSULATION AND DECAPSULATION METHOD AND NUCLEIC ACID STORAGE MICRO-FLUIDIC CHIP
Document Type and Number:
WIPO Patent Application WO/2023/202368
Kind Code:
A1
Abstract:
Disclosed are a nucleic acid encapsulation and decapsulation method and a nucleic acid storage micro-fluidic chip. In a first aspect, provided is a nucleic acid encapsulation method. The encapsulation method comprises the following steps: taking and mixing an organic ligand, a metal ion, and a nanosphere having at least one nucleic acid fragment immobilized on the surface, reacting the organic ligand with the metal ion to generate a metal organic framework, and forming an encapsulation layer on the surface of the nanosphere to obtain an encapsulated nanosphere. The nanosphere is used as a carrier of the nucleic acid fragment, and the metal organic framework is encapsulated by means of the organic ligand and the metal ion, such that an encapsulation layer is rapidly formed on the surface of the carrier, thus realizing the protection of the nucleic acid fragment encapsulated in the encapsulation layer, with good protection against free radicals. ultraviolet rays, and the like. The whole encapsulation process is short in consumed time and easy to operate, and a highly toxic substance does not need to be used for processing the encapsulation layer in the subsequent decapsulation process, such that the whole encapsulation and decapsulation process is safer.
Inventors:
JIANG XINGYU (CN)
MAO CUIPING (CN)
MAO CUIPING (CN)
Application Number:
PCT/CN2023/085996
Publication Date:
October 26, 2023
Filing Date:
April 03, 2023
Export Citation:
Assignee:
UNIV SOUTHERN SCI & TECH (CN)
International Classes:
B01L3/00; C12N15/10; C08G83/00; C12Q1/6806; C12Q1/6869
Foreign References:
| CN114717227A | 2022-07-08 | |||
| CN217549839U | 2022-10-11 | |||
| CN107486177A | 2017-12-19 | |||
| CN111110846A | 2020-05-08 | |||
| CN112840036A | 2021-05-25 | |||
| CN113293159A | 2021-08-24 | |||
| CN115970773A | 2023-04-18 |
Other References:
JINGJING WANG, FANGNAN XIAO, YUNKUN WU: "Metal-Organic Frameworks and Novel Drug Loading System", CHEMISTRY, vol. 85, no. 3, 7 December 2021 (2021-12-07), pages 297 - 302, XP093102152, DOI: 10.14159/j.cnki.0441-3776.20211206.003
YANTAO LI; KAI ZHANG; PORUN LIU; MO CHEN; YULIN ZHONG; QINGSONG YE; MING Q. WEI; HUIJUN ZHAO; ZHIYONG TANG: "Encapsulation of Plasmid DNA by Nanoscale Metal–Organic Frameworks for Efficient Gene Transportation and Expression", ADVANCED MATERIALS, VCH PUBLISHERS, DE, vol. 31, no. 29, 3 June 2019 (2019-06-03), DE , pages n/a - n/a, XP071874503, ISSN: 0935-9648, DOI: 10.1002/adma.201901570
PODDAR ARPITA, CONESA JOSÉ J., LIANG KANG, DHAKAL SUDIP, REINECK PHILIPP, BRYANT GARY, PEREIRO EVA, RICCO RAFFAELE, AMENITSCH HEIN: "Encapsulation, Visualization and Expression of Genes with Biomimetically Mineralized Zeolitic Imidazolate Framework‐8 (ZIF‐8)", SMALL, WILEY, HOBOKEN, USA, vol. 15, no. 36, 1 September 2019 (2019-09-01), Hoboken, USA, pages 1902268, XP093101705, ISSN: 1613-6810, DOI: 10.1002/smll.201902268
YANTAO LI; KAI ZHANG; PORUN LIU; MO CHEN; YULIN ZHONG; QINGSONG YE; MING Q. WEI; HUIJUN ZHAO; ZHIYONG TANG: "Encapsulation of Plasmid DNA by Nanoscale Metal–Organic Frameworks for Efficient Gene Transportation and Expression", ADVANCED MATERIALS, VCH PUBLISHERS, DE, vol. 31, no. 29, 3 June 2019 (2019-06-03), DE , pages n/a - n/a, XP071874503, ISSN: 0935-9648, DOI: 10.1002/adma.201901570
PODDAR ARPITA, CONESA JOSÉ J., LIANG KANG, DHAKAL SUDIP, REINECK PHILIPP, BRYANT GARY, PEREIRO EVA, RICCO RAFFAELE, AMENITSCH HEIN: "Encapsulation, Visualization and Expression of Genes with Biomimetically Mineralized Zeolitic Imidazolate Framework‐8 (ZIF‐8)", SMALL, WILEY, HOBOKEN, USA, vol. 15, no. 36, 1 September 2019 (2019-09-01), Hoboken, USA, pages 1902268, XP093101705, ISSN: 1613-6810, DOI: 10.1002/smll.201902268
Attorney, Agent or Firm:
JIAQUAN IP LAW (CN)
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