Title:
NUCLEIC ACID EXTRACTION METHOD USING SYRINGE-COLUMN ELUTION SYSTEM
Document Type and Number:
WIPO Patent Application WO/2022/092850
Kind Code:
A1
Abstract:
The present invention relates to a method for simply and rapidly extracting nucleic acids from a sample by using a plurality of syringe columns that are each filled, in advance, with a different buffer. Compared with conventional nucleic acid extraction methods using spin columns, a nucleic acid extraction method according to the present invention remarkably reduces nucleic acid extraction time by using syringes filled with a buffer enabling simultaneous sample dissolution and nucleic acid binding and a plurality of syringe columns filled with wash buffer and elution buffer, and enables the elution of a buffer into a column without the need for an expensive centrifuge by using syringe pressure, and thus is useful during nucleic acid extraction in a clinical setting in which a molecular-biological diagnosis is required.
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Inventors:
PARK HEE KYUNG (KR)
PARK JAE SIN (KR)
LEE SI SEOK (KR)
CHO SEE WUN (KR)
PARK JAE SIN (KR)
LEE SI SEOK (KR)
CHO SEE WUN (KR)
Application Number:
PCT/KR2021/015304
Publication Date:
May 05, 2022
Filing Date:
October 28, 2021
Export Citation:
Assignee:
SEASUN BIOMATERIALS (KR)
International Classes:
C12N15/10; C12Q1/6806
Domestic Patent References:
| WO2020191189A1 | 2020-09-24 |
Foreign References:
| US20030096229A1 | 2003-05-22 | |||
| US5378359A | 1995-01-03 | |||
| US20180355346A1 | 2018-12-13 |
Other References:
KIM YONG-EON: "The Development of the World's First New Concept DNA Extraction Kit", NATIONAL INSTITUTE OF FOREST SCIENCE, 12 May 2020 (2020-05-12), XP055926114, Retrieved from the Internet
Attorney, Agent or Firm:
LEE, Cheo Young et al. (KR)
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