Title:
NUCLEIC ACID ISOLATED FROM THE 5' NON TRANSLATED REGION (5'NTR) END OF THE HUMAN FGF-1 GENE HAVING IRES ACTIVITY
Document Type and Number:
WIPO Patent Application WO2004003209
Kind Code:
A3
Abstract:
The invention concerns an isolated nucleic acid of the human FGF-1 gene having IRES (internal ribosome entry site) activity, said nucleotide sequence being derived from the 5' non translated region (5'NTR) end of the human FGF-1 gene. The invention also concerns an expression cassette of at least one molecule of interest in a eukaryotic cell comprising at least one of said sequences. The cassette can be inserted directly or in the form of a vector in a host cell, hence the invention further concerns the recombinant vectors and the host cell transfected by said vector or the cassette.
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Inventors:
MARTINEAU YVAN (FR)
PRATS ANNE-CATHERINE (FR)
LE BEC CHRISTINE (FR)
PRATS ANNE-CATHERINE (FR)
LE BEC CHRISTINE (FR)
Application Number:
PCT/FR2003/001969
Publication Date:
April 15, 2004
Filing Date:
June 26, 2003
Export Citation:
Assignee:
GENETHON (FR)
INST NAT SANTE RECH MED (FR)
MARTINEAU YVAN (FR)
PRATS ANNE-CATHERINE (FR)
LE BEC CHRISTINE (FR)
INST NAT SANTE RECH MED (FR)
MARTINEAU YVAN (FR)
PRATS ANNE-CATHERINE (FR)
LE BEC CHRISTINE (FR)
International Classes:
A01K67/027; A61K35/76; A61K48/00; A61P35/00; C12N1/15; C12N1/19; C12N1/21; C12N5/08; C12N5/10; C12N15/09; C12N15/12; C12N15/85; (IPC1-7): C12N15/85; A01K67/00; C12N15/12
Other References:
DATABASE EMBL [online] 18 January 1993 (1993-01-18), CHIU I: "H. sapiens gene for acidic fibroblast growth factor, 5' untranslated exon 1A acidic fibroblast growth factor", XP002240944, Database accession no. Z14149
DATABASE EMBL [online] 19 March 1991 (1991-03-19), CRUMLEY G ET AL: "Homo sapiens acidic fibroblast growth factor (FGFA) gene, exon 1", XP002262930, Database accession no. M60520
DATABASE EMBL [online] 9 May 2003 (2003-05-09), BAHR A ET AL: "Homo sapiens mRNA; EST", XP002262931, Database accession no. BX501193
DATABASE EMBL [online] 19 March 1991 (1991-03-19), CRUMLEY G ET AL: "Human acidic fibroblast growht factor mRNA, 5' end", XP002262932, Database accession no. M60515
DATABASE EMBL [online] 19 November 1992 (1992-11-19), MYERS RL ET AL: "H. sapiens fgfA gene for fibroblast growth factor", XP002262933, Database accession no. X59612
DATABASE EMBL 31 July 2001 (2001-07-31), HALLEY C ET AL: "Bos taurus heparin-binding growth factor (aFGF) mRNA, 5' end", XP002262934
DATABASE EMBL [online] 2 October 2001 (2001-10-02), EDWARDS J B D M ET AL: "Sequence tag and encoded human protein", XP002240945, Database accession no. BD044518
DATABASE GENESEQ [online] 6 October 2000 (2000-10-06), DUMAS MILNE EDWARDS J ET AL: "Human secreted protein 5' EST", XP002262935, Database accession no. AAC20773
DATABASE EMBL [online] PAYSON RA ET AL: "Homo sapiens acidic fibroblast growth factor mRNA, 5' end", XP002262936, Database accession no. L01485
DATABASE EMBL [online] 24 January 1992 (1992-01-24), CHIU IM ET AL: "Human FGFA gene STS ocated at 5131.3-33.2", XP002262937, Database accession no. X62586
DATABASE EMBL [online] 18 January 1993 (1993-01-18), CHIU I: "H. sapiens gene for acidic fibroblast growth factor, 5' untranslated exon 1C", XP002240946, Database accession no. Z14151
FLORKIEWICZ R Z ET AL: "HUMAN BASIC FIBROBLAST GROWTH FACTOR GENE ENCODES FOUR POLYPEPTIDES: THREE INITIATE TRANSLATION FROM NON-AUG CODONS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 86, no. 11, 1 June 1989 (1989-06-01), pages 3978 - 3981, XP000026477, ISSN: 0027-8424
CREANCIER LAURENT ET AL: "Fibroblast growth factor 2 internal ribosome entry site (IRES) activity ex vivo and in transgenic mice reveals a stringent tissue-specific regulation.", JOURNAL OF CELL BIOLOGY, vol. 150, no. 1, 10 July 2000 (2000-07-10), pages 275 - 281, XP002240943, ISSN: 0021-9525
DATABASE EMBL [online] 19 March 1991 (1991-03-19), CRUMLEY G ET AL: "Homo sapiens acidic fibroblast growth factor (FGFA) gene, exon 1", XP002262930, Database accession no. M60520
DATABASE EMBL [online] 9 May 2003 (2003-05-09), BAHR A ET AL: "Homo sapiens mRNA; EST", XP002262931, Database accession no. BX501193
DATABASE EMBL [online] 19 March 1991 (1991-03-19), CRUMLEY G ET AL: "Human acidic fibroblast growht factor mRNA, 5' end", XP002262932, Database accession no. M60515
DATABASE EMBL [online] 19 November 1992 (1992-11-19), MYERS RL ET AL: "H. sapiens fgfA gene for fibroblast growth factor", XP002262933, Database accession no. X59612
DATABASE EMBL 31 July 2001 (2001-07-31), HALLEY C ET AL: "Bos taurus heparin-binding growth factor (aFGF) mRNA, 5' end", XP002262934
DATABASE EMBL [online] 2 October 2001 (2001-10-02), EDWARDS J B D M ET AL: "Sequence tag and encoded human protein", XP002240945, Database accession no. BD044518
DATABASE GENESEQ [online] 6 October 2000 (2000-10-06), DUMAS MILNE EDWARDS J ET AL: "Human secreted protein 5' EST", XP002262935, Database accession no. AAC20773
DATABASE EMBL [online] PAYSON RA ET AL: "Homo sapiens acidic fibroblast growth factor mRNA, 5' end", XP002262936, Database accession no. L01485
DATABASE EMBL [online] 24 January 1992 (1992-01-24), CHIU IM ET AL: "Human FGFA gene STS ocated at 5131.3-33.2", XP002262937, Database accession no. X62586
DATABASE EMBL [online] 18 January 1993 (1993-01-18), CHIU I: "H. sapiens gene for acidic fibroblast growth factor, 5' untranslated exon 1C", XP002240946, Database accession no. Z14151
FLORKIEWICZ R Z ET AL: "HUMAN BASIC FIBROBLAST GROWTH FACTOR GENE ENCODES FOUR POLYPEPTIDES: THREE INITIATE TRANSLATION FROM NON-AUG CODONS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 86, no. 11, 1 June 1989 (1989-06-01), pages 3978 - 3981, XP000026477, ISSN: 0027-8424
CREANCIER LAURENT ET AL: "Fibroblast growth factor 2 internal ribosome entry site (IRES) activity ex vivo and in transgenic mice reveals a stringent tissue-specific regulation.", JOURNAL OF CELL BIOLOGY, vol. 150, no. 1, 10 July 2000 (2000-07-10), pages 275 - 281, XP002240943, ISSN: 0021-9525
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