Title:
NUCLEIC ACID LIGASE
Document Type and Number:
WIPO Patent Application WO/2023/273366
Kind Code:
A1
Abstract:
Provided is a nucleic acid ligase, which comprises, compared with an existing amino acid sequence of Hyperligase (shown in SEQ ID NO: 1), an amino acid sequence having a mutation at one or more positions selected from among positions 79, 281, 370 and 372. Also provided are a nucleic acid molecule encoding the enzyme, a carrier containing the nucleic acid molecule, and a recombinant cell containing the nucleic acid molecule or the carrier. Also provided are a composition containing the enzyme and a use of the enzyme.
Inventors:
DING CHUNMING (CN)
YANG ZHENGQUAN (CN)
JIN SHENGNAN (CN)
YANG ZHENGQUAN (CN)
JIN SHENGNAN (CN)
Application Number:
PCT/CN2022/077538
Publication Date:
January 05, 2023
Filing Date:
February 24, 2022
Export Citation:
Assignee:
UNIV WENZHOU MEDICAL (CN)
International Classes:
C12N9/00; C12N15/52; C12P19/34
Domestic Patent References:
| WO2017160788A2 | 2017-09-21 | |||
| WO2017160788A2 | 2017-09-21 |
Foreign References:
| CN113481174A | 2021-10-08 | |||
| CN108220255A | 2018-06-29 | |||
| US20090061481A1 | 2009-03-05 |
Other References:
TANABE MAIKO, ISHINO SONOKO, YOHDA MASAFUMI, MORIKAWA KOSUKE, ISHINO YOSHIZUMI, NISHIDA HIROKAZU: "Structure-Based Mutational Study of an Archaeal DNA Ligase towards Improvement of Ligation Activity", CHEMBIOCHEM, vol. 13, no. 17, 26 November 2012 (2012-11-26), pages 2575 - 2582, XP093018163, ISSN: 1439-4227, DOI: 10.1002/cbic.201200336
JEON, H. J. ET AL.: "Mutational Analyses of the Thermostable NAD(+)-dependent DNA Ligase from Thermus Filiformis", FEMS MICROBIOLOGY LETTERS, vol. 237, no. 1, 1 August 2004 (2004-08-01), XP002350100, ISSN: 0378-1097, DOI: 10.1111/j.1574-6968.2004.tb09685.x
VIOLLET SFUCHS R TMUNAFO D B ET AL.: "T4 RNA ligase 2 truncated active site mutants: improved tools for RNA analysis [J", BMC BIOTECHNOL, vol. 11, 2011, pages 72, XP021105283, DOI: 10.1186/1472-6750-11-72
LOHMAN G JTABOR SNICHOLS N M: "DNA ligases [J", CURR PROTOC MOL BIOL, 2011
JOHNSON AO'DONNELL M.: "DNA ligase: Getting a grip to seal the deal [J", CURRENT BIOLOGY, vol. 15, no. 3, 2005, pages R90 - R2, XP025939523, DOI: 10.1016/j.cub.2005.01.025
CHEREPANOV A VDE VRIES S: "Dynamic mechanism of nick recognition by DNA ligase [J", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 269, no. 24, 2002, pages 5993 - 9
TUMBALE P PJURKIW T JSCHELLENBERG M J ET AL.: "Two-tiered enforcement of high-fidelity DNA ligation [J", NATURE COMMUNICATIONS, vol. 10, no. 1, 2019, pages 5431
FOY C APARKES H C: "Emerging Homogeneous DNA-based Technologies in the Clinical Laboratory [J", CLINICAL CHEMISTRY, vol. 47, no. 6, 2001, pages 990 - 1000
BLONDAL TTHORISDOTTIR AUNNSTEINSDOTTIR U ET AL.: "Isolation and characterization of a thermostable RNA ligase 1 from a Thermus scotoductus bacteriophage TS2126 with good single-stranded DNA ligation properties [J", NUCLEIC ACIDS RES, vol. 33, no. 1, 2005, pages 135 - 42, XP003026551, DOI: 10.1093/NAR/GKI149
ZHELKOVSKY A MMCREYNOLDS L A: "Structure-function analysis of Methanobacterium thermoautotrophicum RNA ligase - engineering a thermostable ATP independent enzyme [J", BMC MOLECULAR BIOLOGY, vol. 13, no. 1, 2012, pages 10, XP021128041, DOI: 10.1186/1471-2199-13-24
JEON, H. J. ET AL.: "Mutational Analyses of the Thermostable NAD(+)-dependent DNA Ligase from Thermus Filiformis", FEMS MICROBIOLOGY LETTERS, vol. 237, no. 1, 1 August 2004 (2004-08-01), XP002350100, ISSN: 0378-1097, DOI: 10.1111/j.1574-6968.2004.tb09685.x
VIOLLET SFUCHS R TMUNAFO D B ET AL.: "T4 RNA ligase 2 truncated active site mutants: improved tools for RNA analysis [J", BMC BIOTECHNOL, vol. 11, 2011, pages 72, XP021105283, DOI: 10.1186/1472-6750-11-72
LOHMAN G JTABOR SNICHOLS N M: "DNA ligases [J", CURR PROTOC MOL BIOL, 2011
JOHNSON AO'DONNELL M.: "DNA ligase: Getting a grip to seal the deal [J", CURRENT BIOLOGY, vol. 15, no. 3, 2005, pages R90 - R2, XP025939523, DOI: 10.1016/j.cub.2005.01.025
CHEREPANOV A VDE VRIES S: "Dynamic mechanism of nick recognition by DNA ligase [J", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 269, no. 24, 2002, pages 5993 - 9
TUMBALE P PJURKIW T JSCHELLENBERG M J ET AL.: "Two-tiered enforcement of high-fidelity DNA ligation [J", NATURE COMMUNICATIONS, vol. 10, no. 1, 2019, pages 5431
FOY C APARKES H C: "Emerging Homogeneous DNA-based Technologies in the Clinical Laboratory [J", CLINICAL CHEMISTRY, vol. 47, no. 6, 2001, pages 990 - 1000
BLONDAL TTHORISDOTTIR AUNNSTEINSDOTTIR U ET AL.: "Isolation and characterization of a thermostable RNA ligase 1 from a Thermus scotoductus bacteriophage TS2126 with good single-stranded DNA ligation properties [J", NUCLEIC ACIDS RES, vol. 33, no. 1, 2005, pages 135 - 42, XP003026551, DOI: 10.1093/NAR/GKI149
ZHELKOVSKY A MMCREYNOLDS L A: "Structure-function analysis of Methanobacterium thermoautotrophicum RNA ligase - engineering a thermostable ATP independent enzyme [J", BMC MOLECULAR BIOLOGY, vol. 13, no. 1, 2012, pages 10, XP021128041, DOI: 10.1186/1471-2199-13-24
Attorney, Agent or Firm:
CHINA PATENT AGENT (H.K.) LTD. (CN)
Download PDF: