Title:
NUCLEOTIDES AND POLYNUCLEOTIDES IMMOBILISED ON A SOLID SUPPORT VIA A DISULPHIDE LINKAGE
Document Type and Number:
WIPO Patent Application WO2000053616
Kind Code:
A3
Abstract:
A method of immobilising a nucleotide or polynucleotide on a solid support, comprises: providing the solid support having a functionalised surface of the formula B - S - S - L where B is the solid support and L is a leaving group; providing a nucleotide or polynucleotide comprising at least one phosphorothioate group; and coupling the two together by means of a sulphide exchange reaction. The product has the structure B - S - S - P(O)R<1>R<2>, where - POR<1>R<2> represents a nucleotide or a polynucleotide. Arrays of the immobilised polynucleotides are useful for performing APEX or SBH assays.
Inventors:
ROSLER ANGELIKA JOSEFINE (GB)
Application Number:
PCT/GB2000/000843
Publication Date:
September 12, 2002
Filing Date:
March 07, 2000
Export Citation:
Assignee:
AMERSHAM PHARM BIOTECH UK LTD (GB)
ROSLER ANGELIKA JOSEFINE (GB)
ROSLER ANGELIKA JOSEFINE (GB)
International Classes:
C12N15/09; C07H19/10; C07H19/20; C07H21/00; C07H21/04; C12M1/00; C12Q1/68; G01N33/543; (IPC1-7): C07H21/00; C07H19/10; C07H19/20; C12Q1/68; G01N33/543
Domestic Patent References:
| WO1998039481A1 | 1998-09-11 | |||
| WO1990001564A1 | 1990-02-22 |
Other References:
P.KUMAR ET AL.: "A Versatile Solid Phase Method for the Synthesis of Oligonucleotide-3'-Phosphates.", TETRAHEDRON LETTERS., vol. 32, no. 6, 4 February 1991 (1991-02-04), ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM., NL, pages 967 - 970, XP002112931, ISSN: 0040-4039
MILLOT M C ET AL: "HPLC DETERMINATION OF BIOLOGICAL THIOLS USING POLYMER-COATED SOLID -PHASE REAGENTS CARRYING DISULFIDE FUNCTIONS", REACTIVE POLYMERS, vol. 13, no. 1 + 02, 1 September 1990 (1990-09-01), pages 177 - 190, XP000160693, ISSN: 0923-1137
H.SALO ET AL.: "Disulfide-Tethered Solid Supports for Synthesis of Photoluminescent Oligonucleotide Conjugates : Hydrolytic Stability and Labelling on the Support.", BIOCONJUGATE CHEMISTRY., vol. 9, no. 3, May 1998 (1998-05-01) - June 1998 (1998-06-01), AMERICAN CHEMICAL SOCIETY, WASHINGTON., US, pages 365 - 371, XP002112924, ISSN: 1043-1802
CHASSIGNOL M ET AL: "Phosphodisulfide Bond: A New Linker for the Oligonucleotide Conjugation", TETRAHEDRON LETTERS, vol. 39, no. 45, 5 November 1998 (1998-11-05), pages 8271-8274, XP004139391, ISSN: 0040-4039
S.ALEFELDER ET AL.: "Incorporation of Terminal Phosphorothioates into Oligonucleotides.", NUCLEIC ACIDS RESEARCH., vol. 26, no. 21, 1 November 1998 (1998-11-01), OXFORD UNIVERSITY PRESS, SURREY., GB, pages 4983 - 4988, XP002112925, ISSN: 0305-1048
MILLOT M C ET AL: "HPLC DETERMINATION OF BIOLOGICAL THIOLS USING POLYMER-COATED SOLID -PHASE REAGENTS CARRYING DISULFIDE FUNCTIONS", REACTIVE POLYMERS, vol. 13, no. 1 + 02, 1 September 1990 (1990-09-01), pages 177 - 190, XP000160693, ISSN: 0923-1137
H.SALO ET AL.: "Disulfide-Tethered Solid Supports for Synthesis of Photoluminescent Oligonucleotide Conjugates : Hydrolytic Stability and Labelling on the Support.", BIOCONJUGATE CHEMISTRY., vol. 9, no. 3, May 1998 (1998-05-01) - June 1998 (1998-06-01), AMERICAN CHEMICAL SOCIETY, WASHINGTON., US, pages 365 - 371, XP002112924, ISSN: 1043-1802
CHASSIGNOL M ET AL: "Phosphodisulfide Bond: A New Linker for the Oligonucleotide Conjugation", TETRAHEDRON LETTERS, vol. 39, no. 45, 5 November 1998 (1998-11-05), pages 8271-8274, XP004139391, ISSN: 0040-4039
S.ALEFELDER ET AL.: "Incorporation of Terminal Phosphorothioates into Oligonucleotides.", NUCLEIC ACIDS RESEARCH., vol. 26, no. 21, 1 November 1998 (1998-11-01), OXFORD UNIVERSITY PRESS, SURREY., GB, pages 4983 - 4988, XP002112925, ISSN: 0305-1048
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