Title:
OLIGO-MODIFIED NUCLEOTIDE ANALOGUES FOR NUCLEIC ACID PREPARATION
Document Type and Number:
WIPO Patent Application WO/2022/251510
Kind Code:
A3
Abstract:
Nucleic acid techniques are disclosed. Embodiments include modified nucleotides 12 with oligonucleotide adapters 24 that are coupled via cleavable linkers 20. Incorporation of the modified nucleotide 12 at a 3' end of a nucleic acid permits end-adapterization via ligation of a free 5' end of the oligonucleotide adapter 24 to a 3' reactive group of the modified nucleotide 12 and cleavage at the cleavable linker 20 to liberate a free 3' end.
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Inventors:
GORMLEY NIALL (GB)
RANDISE-HINCHLIFF CARLO (US)
BRODIN JEFFREY (US)
MUSGRAVE-BROWN ESTHER (GB)
SHULTZABERGER SARAH (US)
SLATTER ANDREW (GB)
FISHER JEFFREY (US)
RANDISE-HINCHLIFF CARLO (US)
BRODIN JEFFREY (US)
MUSGRAVE-BROWN ESTHER (GB)
SHULTZABERGER SARAH (US)
SLATTER ANDREW (GB)
FISHER JEFFREY (US)
Application Number:
PCT/US2022/031150
Publication Date:
January 05, 2023
Filing Date:
May 26, 2022
Export Citation:
Assignee:
ILLUMINA INC (US)
ILLUMINA CAMBRIDGE LTD (GB)
ILLUMINA CAMBRIDGE LTD (GB)
International Classes:
C12Q1/6806; C12Q1/6869
Domestic Patent References:
| WO2018075785A1 | 2018-04-26 |
Foreign References:
| US20080050731A1 | 2008-02-28 | |||
| EP3036359A2 | 2016-06-29 |
Other References:
ROUTH ANDREW ET AL: "ClickSeq: Fragmentation-Free Next-Generation Sequencing via Click Ligation of Adaptors to Stochastically Terminated 3'-Azido c", JOURNAL OF MOLECULAR BIOLOGY, ACADEMIC PRESS, UNITED KINGDOM, vol. 427, no. 16, 24 June 2015 (2015-06-24), pages 2610 - 2616, XP029252591, ISSN: 0022-2836, DOI: 10.1016/J.JMB.2015.06.011
Attorney, Agent or Firm:
BAKKER, Jila et al. (US)
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