Title:
OLIGONUCLEOTIDE-TYRAMIDE CONJUGATE AND USE OF THE SAME IN TYRAMIDE-SIGNAL AMPLIFICATION (TSA)-BASED DETECTION METHODS
Document Type and Number:
WIPO Patent Application WO/2021/226516
Kind Code:
A3
Abstract:
Provided herein, among other things, is an oligonucleotide-tyramide conjugate, which conjugate can be used in variety of tyramide-signal amplification (TSA)-based detection methods. Some embodiments of the method may comprise incubating a sample that has a binding agent-peroxidase conjugate bound to sites thereon (where the peroxidase may directly linked to the binding agent or indirectly directly linked to the binding agent via a pair of oligonucleotides that are hybridized together) with an oligonucleotide-tyramide conjugate in the presence of hydrogen peroxide, thereby activating the tyramide and causing deposition of the oligonucleotide of the oligonucleotide-tyramide conjugate onto sites that are proximal to the peroxidase of the binding agent-peroxidase conjugate.
Inventors:
NOLAN GARRY P (US)
KINOSHITA SHIGEMI (US)
KINOSHITA SHIGEMI (US)
Application Number:
PCT/US2021/031380
Publication Date:
December 16, 2021
Filing Date:
May 07, 2021
Export Citation:
Assignee:
UNIV LELAND STANFORD JUNIOR (US)
International Classes:
C12Q1/68; G01N33/53
Foreign References:
| US20180372736A1 | 2018-12-27 | |||
| US20190018018A1 | 2019-01-17 |
Attorney, Agent or Firm:
KEDDIE, James S. (US)
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