OPTICAL MEMBERS AND MICROSCOPE

WIPO Patent Application WO/2012/002542

A1

Disclosed are optical members and a microscope that enable brighter, sharper images for observation to be obtained when fluorescence imaging is performed with photic stimulation. Illuminating light from a laser unit (21) is separated into stimulating light and excitation light by a dichroic mirror (40A). In other words, half of the illuminating light passes through the dichroic mirror (40A) and becomes stimulating light, and half of the illuminating light is reflected by the dichroic mirror (40A) and becomes excitation light. Half of the excitation light is reflected by a dichroic mirror (43A) and irradiates a sample (14), and half of the stimulating light passes through the dichroic mirror (43A) and irradiates the sample (14). The fluorescence produced from the sample (14) is completely reflected by the dichroic mirror (43A) and the dichroic mirror (40A), and is received by a photodetector (48). By separating the stimulating light and the excitation light at a specified ratio, and using dichroic mirrors to completely reflect fluorescence, a reduction in the amount of fluorescence can be minimized. The optical members and microscope can be used in a confocal microscope.

SATAKA RYOICHI (JP)
HAGIWARA MAYUMI (JP)

PCT/JP2011/065214

January 05, 2012

July 01, 2011

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NIKON CORP (JP)
SATAKA RYOICHI (JP)
HAGIWARA MAYUMI (JP)

G01N21/64; G02B5/28; G02B21/06

WO2008004336A1

2008-01-10

JP2009238990A	2009-10-15
JP2006106346A	2006-04-20
JPH05232314A	1993-09-10
JPH0672114U	1994-10-07
JPH09101110A	1997-04-15
JP2009008554A	2009-01-15
JPH10153705A	1998-06-09
JPH10206742A	1998-08-07
JP2008276191A	2008-11-13

INAMOTO Yoshio et al. (JP)
Yoshio Inemoto (JP)

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