Title:
OPTIMAL METHOD FOR PURIFICATION OF COLLAGEN FROM EXTRACELLULAR MATRIX
Document Type and Number:
WIPO Patent Application WO/2023/055043
Kind Code:
A1
Abstract:
The present invention relates to a method for the purification of collagen from the extracellular matrix, the method comprising the steps of: (a) destaining the extracellular matrix; (b) eliminating non-collagenous proteins from the destained extracellular matrix; (c) preparing atelocollagen from the collagen; and (d) hydrolyzing the prepared atelocollagen.
Inventors:
CHUN SO YOUNG (KR)
KWON TAE GYUN (KR)
KIM BUM SOO (KR)
LEE JUN NYUNG (KR)
HA YUN SOK (KR)
KIM HYUN TAE (KR)
LEE EUN HYE (KR)
CHUNG JAE WOOK (KR)
KWON TAE GYUN (KR)
KIM BUM SOO (KR)
LEE JUN NYUNG (KR)
HA YUN SOK (KR)
KIM HYUN TAE (KR)
LEE EUN HYE (KR)
CHUNG JAE WOOK (KR)
Application Number:
PCT/KR2022/014502
Publication Date:
April 06, 2023
Filing Date:
September 28, 2022
Export Citation:
Assignee:
KYUNGPOOK NAT UNIV IND ACADEMIC COOP FOUND (KR)
International Classes:
C07K14/78; C07K1/12; C12N5/071
Foreign References:
| CN113164645A | 2021-07-23 | |||
| CN105601731A | 2016-05-25 |
Other References:
BEOB SOO KIM; JI SUK CHOI; JAE DONG KIM; HWA IN YOON; YOUNG CHAN CHOI; YONG WOO CHO: "Human collagen isolated from adipose tissue", BIOTECHNOLOGY PROGRESS, AMERICAN CHEMICAL SOCIETY, HOBOKEN, USA, vol. 28, no. 4, 8 June 2012 (2012-06-08), Hoboken, USA, pages 973 - 980, XP072294051, ISSN: 8756-7938, DOI: 10.1002/btpr.1555
CHUN SO YOUNG, LEE JUN NYUNG, HA YUN-SOK, YOON BO HYUN, LEE EUN HYE, KIM BO MI, GIL HAEJUNG, HAN MAN-HOON, OH WOO SEOK, KWON TAE G: "Optimization of extracellular matrix extraction from human perirenal adipose tissue", JOURNAL OF BIOMATERIALS APPLICATIONS., TECHNOMIC, LANCASTER, PA., US, vol. 35, no. 9, 1 April 2021 (2021-04-01), US , pages 1180 - 1191, XP093054236, ISSN: 0885-3282, DOI: 10.1177/0885328220984594
DA SILVA CASSIA MARIA LINS; SPINELLI ELIANI; RODRIGUES SILVANA VIANNA: "Fast and sensitive collagen quantification by alkaline hydrolysis/hydroxyproline assay", FOOD CHEMISTRY, ELSEVIER LTD., NL, vol. 173, 22 October 2014 (2014-10-22), NL , pages 619 - 623, XP029102190, ISSN: 0308-8146, DOI: 10.1016/j.foodchem.2014.10.073
CHUN SO YOUNG, LEE JUN NYUNG, HA YUN-SOK, YOON BO HYUN, LEE EUN HYE, KIM BO MI, GIL HAEJUNG, HAN MAN-HOON, OH WOO SEOK, KWON TAE G: "Optimization of extracellular matrix extraction from human perirenal adipose tissue", JOURNAL OF BIOMATERIALS APPLICATIONS., TECHNOMIC, LANCASTER, PA., US, vol. 35, no. 9, 1 April 2021 (2021-04-01), US , pages 1180 - 1191, XP093054236, ISSN: 0885-3282, DOI: 10.1177/0885328220984594
DA SILVA CASSIA MARIA LINS; SPINELLI ELIANI; RODRIGUES SILVANA VIANNA: "Fast and sensitive collagen quantification by alkaline hydrolysis/hydroxyproline assay", FOOD CHEMISTRY, ELSEVIER LTD., NL, vol. 173, 22 October 2014 (2014-10-22), NL , pages 619 - 623, XP029102190, ISSN: 0308-8146, DOI: 10.1016/j.foodchem.2014.10.073
Attorney, Agent or Firm:
PADO IP LAW PLLC (KR)
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