Title:
OPTIMIZED CAS PROTEIN AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/173682
Kind Code:
A1
Abstract:
The present invention relates to the field of nucleic acid editing, and in particular, to the technical field of clustered regularly interspaced short palindromic repeats (CRISPR); and specifically provides an optimized Cas protein and the use thereof, which have wide application prospects.
Inventors:
DUAN ZHIQIANG (CN)
Application Number:
PCT/CN2022/113989
Publication Date:
September 21, 2023
Filing Date:
August 22, 2022
Export Citation:
Assignee:
SHANDONG SHUNFENG BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12N9/22; C12N15/113; C12N15/55; C12Q1/6816
Foreign References:
| CN114672473A | 2022-06-28 | |||
| CN111757889A | 2020-10-09 | |||
| CN113106081A | 2021-07-13 | |||
| CN112195164A | 2021-01-08 | |||
| CN113897416A | 2022-01-07 | |||
| US20200216825A1 | 2020-07-09 | |||
| CN112391446A | 2021-02-23 |
Other References:
TAKEDA, S.N. ET AL.: "Structure of the Miniature Type V-F CRISPR-Cas Effector Enzyme.", MOLECULAR CELL., vol. 81, 4 February 2021 (2021-02-04), pages 558 - 570, XP086487104, DOI: 10.1016/j.molcel.2020.11.035
XIAO RENJIAN, LI ZHUANG, WANG SHUKUN, HAN RUIJIE, CHANG LEIFU: "Structural basis for the dimerization-dependent CRISPR-Cas12f nuclease", BIORXIV, 22 December 2020 (2020-12-22), pages 1 - 20, XP093090355, DOI: 10.1101/2020.12.22.424058
WANG YANCHUN, SANG SHULI, ZHANG XIN, TAO HAOXIA, GUAN QING, LIU CHUNJIE: "Efficient Genome Editing by a Miniature CRISPR-AsCas12f1 Nuclease in Bacillus anthracis", FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, vol. 9, XP093090359, DOI: 10.3389/fbioe.2021.825493
XIAO RENJIAN, LI ZHUANG, WANG SHUKUN, HAN RUIJIE, CHANG LEIFU: "Structural basis for the dimerization-dependent CRISPR-Cas12f nuclease", BIORXIV, 22 December 2020 (2020-12-22), pages 1 - 20, XP093090355, DOI: 10.1101/2020.12.22.424058
WANG YANCHUN, SANG SHULI, ZHANG XIN, TAO HAOXIA, GUAN QING, LIU CHUNJIE: "Efficient Genome Editing by a Miniature CRISPR-AsCas12f1 Nuclease in Bacillus anthracis", FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, vol. 9, XP093090359, DOI: 10.3389/fbioe.2021.825493
Attorney, Agent or Firm:
BEIJING JUNHUI INTELLECTUAL PROPERTY AGENT OFFICE (ORDINARY PARTNERSHIP) (CN)
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